CN217442965U - Automatic drip dyeing device for gram dyeing - Google Patents

Automatic drip dyeing device for gram dyeing Download PDF

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Publication number
CN217442965U
CN217442965U CN202221033446.0U CN202221033446U CN217442965U CN 217442965 U CN217442965 U CN 217442965U CN 202221033446 U CN202221033446 U CN 202221033446U CN 217442965 U CN217442965 U CN 217442965U
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reagent
dyeing
slide
gram
reagent bottle
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李小琴
杨彦龙
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First Affiliated Hospital of Chongqing Medical University
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First Affiliated Hospital of Chongqing Medical University
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Abstract

The utility model discloses an automatic device that dyes that drips of gram dyeing belongs to bacterial dyeing equipment field. The automatic drip dyeing device for the gram dyeing comprises: the reagent sample adding device comprises a plurality of reagent bottles and a plurality of conduits, and each reagent bottle is communicated with the reagent dripping controller through one conduit; the slide fixing device is of a plate-shaped plane structure and is fixed below the reagent dripping controller, the platform angle rotating device is installed on the slide fixing device, and the upper part of the platform angle rotating device is rotatably connected with the bottom surface of the slide clamping groove table; a dropper is arranged below the reagent dripping controller, and the lower part of the dropper corresponds to one end of the slide clamping groove platform; the whole device controls the dye liquor in different reagent bottles to flow out and the amount of the dye liquor to flow out through a reagent dripping controller; when the slide clamping groove table rotates for a certain angle, the dyeing liquid is gradually dip-dyed from one end to the other end in the slide clamping groove table to finish dyeing; manpower and material resources are saved, the speed is high, and large batch of sample operation can be realized.

Description

Automatic drip dyeing device for gram dyeing
Technical Field
The utility model relates to a bacterial dyeing equipment field especially relates to an automatic device that dyes that drips of gram dyeing.
Background
Gram staining is a method for distinguishing germs; depending on the physical properties of the microorganisms on the bacterial cell wall, pathogens can be classified into two broad categories, namely purple-staining gram-positive bacteria and red-staining gram-negative bacteria. The typical purple and red color that appears after staining is very important for the discrimination of bacteria. The existing dyeing method mainly comprises a manual dyeing method (namely a drop dyeing method which has good dyeing effect but is complicated to operate and inconvenient for mass samples) and instrument dyeing (mainly spray dyeing which can be used for mass dyeing but has poor dyeing effect).
Aiming at the defects of the existing gram staining instrument, a gram staining device which is simple to operate, can be automatically carried out and is suitable for mass samples is needed.
SUMMERY OF THE UTILITY MODEL
In order to realize the purpose, the utility model provides an automatic drip dyeing device for gram dyeing, simple structure, labour saving and time saving, convenient to use.
The utility model provides an above-mentioned technical problem's technical scheme as follows: an automatic drip dyeing device for gram dyeing comprises:
the device comprises a reagent sample adding device, a reagent dripping controller, a slide fixing device, a slide clamping groove table and a platform angle rotating device;
the reagent sample adding device comprises a plurality of reagent bottles and a plurality of guide pipes which are in one-to-one correspondence with the reagent bottles, each reagent bottle is respectively communicated with the reagent dripping controller through the corresponding guide pipe, and the reagent dripping controller is arranged below the reagent sample adding device;
the slide fixing device is of a plate-shaped flat plate structure and is fixed below the reagent dripping controller, the platform angle rotating device is installed on the slide fixing device, and the slide clamping groove table is obliquely and rotatably installed on the platform angle rotating device, wherein one end of the slide clamping groove table is higher and the other end of the slide clamping groove table is lower;
a dropper is arranged below the reagent dripping controller, and liquid is dripped to the high position in the slide clamping groove table below the dropper.
The utility model has the advantages that: controlling the dye solution in different reagent bottles in the reagent adding device to flow out and the amount of the dye solution to flow out through a reagent adding controller; wherein, the sample is placed in the slide clamping groove table, one end of the slide clamping groove table is higher, and the other end is lower, so that the dyeing liquid is gradually dip-dyed from the high position to the low position in the slide clamping groove table to complete the dyeing of the sample; the slide slot table is rotatably mounted on the platform angle rotating device, and the inclination angle of the slide slot table can be adjusted through the platform angle rotating device. The whole device is highly automatic, saves manpower and material resources, is high in speed, and can realize large-batch sample operation.
On the basis of the technical scheme, the utility model discloses can also do as follows the improvement:
further, the automatic drip dyeing device for the gram dyeing further comprises a reagent bottle rack, wherein a plurality of grooves are formed in the reagent bottle rack, and the reagent bottles are placed in the grooves in an inverted mode in one-to-one correspondence.
The beneficial effect of adopting the further scheme is that: the reagent bottle rack is used for fixing the inverted reagent bottle, so that the dye liquor in the reagent bottle can flow out conveniently, the reagent bottle can be tightly clamped by the groove, and the reagent bottle can be kept stable in the process of flowing out the liquid from the guide pipe.
Furthermore, there are five reagent bottles, which are respectively a crystal violet reagent bottle, an iodine solution reagent bottle, a clear water reagent bottle, an ethanol reagent bottle and a saffron reagent bottle.
The beneficial effect of adopting the further scheme is that: five reagent bottles correspond to five liquids, one of which is clear water and is used for removing residual reagents in the slide card slot platform and the dropper after each dyeing.
Furthermore, the automatic drip dyeing device for the gram dyeing further comprises a shell, the shell is in a transparent box shape, and the shell covers the reagent sample adding device.
The beneficial effect of adopting the further scheme is that: the shell of the transparent box can prevent dye liquor from splashing outwards to pollute the environment, and meanwhile, the internal flow can be clearly checked through the transparent arrangement.
Furthermore, a plurality of openings are formed in the reagent dripping controller, the openings are communicated with the plurality of guide pipes in a one-to-one correspondence mode, the openings are communicated with the dropper, and the reagent dripping controller is further provided with a control switch used for opening or closing a single opening.
The beneficial effect of adopting the above further scheme is: the reagent dropwise add controller controls the switching order and the time of a plurality of trompils, and then controls how much of different dye liquors in different reagent bottles flow out, and the dye liquor flows into the burette after passing through the trompil again, falls into the one end of slide card slot platform from the burette at last, and whole process reasonable in design has reduced artifical loaded down with trivial details operation many times.
Furthermore, a vent pipe is communicated with the joint of the upper end of the guide pipe and the reagent bottle, and the vent pipe is communicated with the outside and the reagent bottle and is used for balancing the pressure of the reagent bottle and the outside.
The beneficial effect of adopting the further scheme is that: the breather pipe is used for balancing the air pressure between the interior of the reagent bottle and the outside, and then the liquid in the reagent bottle can flow out easily.
Further, the platform angle rotating device comprises a motor, a transmission gear set and a controller; the glass slide fixing device is characterized in that the motor and the transmission gear set are both installed on the glass slide fixing device, the driving end of the motor is meshed with the input end of the transmission gear set, the output end of the transmission gear set is connected with and drives the glass slide clamping groove table to rotate, and the controller is connected with and controls the motor to rotate.
The beneficial effect of adopting the further scheme is that: the motor rotates slide draw-in groove platform after reducing speed through drive gear group, and wherein the controller control motor pivoted is what, and then realizes the required angle of slide draw-in groove platform slope, makes things convenient for the dye liquor to smoothly flow down, accomplishes once dyeing to the sample.
Further, the slide draw-in groove platform is box-shaped structure, and inside lower extreme is equipped with the draw-in groove, the slope the low department of slide draw-in groove platform still is equipped with the waste liquid water conservancy diversion mouth that is used for the waste liquid to flow.
The beneficial effect of adopting the further scheme is that: slide draw-in groove platform is box-shaped structure and conveniently places the slide of taking the sample and dyes the experiment, and the dye liquor can not splash to the outside, and the slide joint is in the draw-in groove simultaneously, and is convenient fixed to whole slide draw-in groove platform rotates the rocking that the in-process can not lead to the slide after dyeing.
Further, the slide card slot table also comprises a slide, and the slide can be detachably clamped in the slide card slot table.
The beneficial effect of adopting the further scheme is that: the slide can be dismantled the joint and in slide draw-in groove platform, and the convenience is after accomplishing at every turn, and the sample slide that carries out the renewal enters.
Further, the automatic drip dyeing device for the gram dyeing further comprises a waste liquid box, and the waste liquid box is installed below the waste liquid diversion port.
The beneficial effect of adopting the further scheme is that: the waste liquid box is used for collecting waste liquid formed after dyeing each time, so that the experimental environment is protected, and meanwhile, the waste liquid can be recycled through other procedures.
Drawings
Fig. 1 is a schematic view of the overall structure of the present invention.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a reagent sample adding device; 11. a reagent bottle; 111. crystal violet reagent bottle; 112. iodine solution reagent bottle; 113. a clear water reagent bottle; 114. an ethanol reagent bottle; 115. a saffron reagent bottle; 12. a conduit; 2. a reagent dripping controller; 21. a dropper; 3. a slide fixing device; 4. a slide slot station; 5. a platform angle rotating device; 6. a waste liquid box; 7. a housing.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the following detailed description of the present invention with reference to the accompanying drawings is not intended to limit the scope of the present invention.
Example 1: as shown in fig. 1, the utility model discloses an embodiment of a gram dyeing automatic drip dyeing device, include:
the device comprises a reagent sample adding device 1, a reagent dripping controller 2, a slide fixing device 3, a slide clamping groove platform 4 and a platform angle rotating device 5;
the reagent sampling device 1 comprises a plurality of reagent bottles 11 and a plurality of guide tubes 12 corresponding to the reagent bottles 11 one by one, each reagent bottle 11 is respectively communicated with a reagent dripping controller 2 through the corresponding guide tube 12, and the reagent dripping controller 2 is arranged below the reagent sampling device 1;
the slide fixing device 3 is of a plate-shaped flat plate structure and is fixed below the reagent dripping controller 2, the platform angle rotating device 5 is installed on the slide fixing device 3, and the slide clamping groove table 4 is obliquely and rotatably installed on the platform angle rotating device 5, wherein one end of the slide clamping groove table is high and the other end of the slide clamping groove table is low;
a dropper 21 is arranged below the reagent dripping controller 2, and liquid is dripped below the dropper 21 to the high position in the slide clamping groove table 4.
Specifically, the reagent sample adding device 1 is positioned above the reagent dripping controller 2, so that the reagent bottles 11 can conveniently flow liquid downwards to the reagent dripping controller 2 through the guide tubes 12, dye liquor in a plurality of reagent bottles 11 passes through the reagent dripping controller 2 and then drops at the high position of the inclined slide clamping groove table 4 through the dropper 21, the dye liquor is gradually impregnated from the high position to the low position in the slide clamping groove table 4, and the dye liquor helps the sample to be more fully dyed under the flowing condition; meanwhile, the slide fixing device 3 is installed on the platform angle rotating device 5, so that the stability of the rotating process is ensured, the inclination angle of the slide clamping groove table 4 is adjusted by rotating the platform angle rotating device 5, the circulation speed of liquid in the slide clamping groove table 4 is facilitated, the platform angle rotating device 5 is installed on the slide fixing device 3, and the stability of the rotating process of the platform angle rotating device 5 is improved.
It should be understood that the reagent dripping controller 2 controls the dye solution in different reagent bottles 11 in the reagent sample adding device 1 to flow out and how much dye solution flows out; wherein, a sample to be dyed is placed on the slide clamping groove table 4, the smear area can be quickly covered by dyeing in a drop dyeing self-flowing mode, and the reagent dosage is saved; the whole device is highly automatic, saves manpower and material resources, is high in speed, and can realize large-batch sample operation.
Preferably, the platform angle rotating means 5 rotates the slide card slot table 4 by 10-15 degrees during dyeing.
Preferably, the dropper 21 is further provided with an adjusting switch for adjusting the size of the liquid flowing out from the dropper 21, so as to ensure that the sample in the slide clamping groove table 4 is fully impregnated.
On the basis of example 1, example 2: as shown in fig. 1: the automatic drip dyeing device for the gram dyeing further comprises a reagent bottle rack, a plurality of grooves are formed in the reagent bottle rack, and a plurality of reagent bottles 11 are inversely placed in the grooves in a one-to-one correspondence mode.
It should be understood that the reagent bottle rack clamps the inverted reagent bottle 11 through the groove, so as to ensure that the reagent bottle 11 is more stable when the liquid in the guide bottle is guided.
Preferably, the height of the groove of the reagent bottle holder is higher than that of the reagent dripping controller 2 so as to facilitate the liquid in the reagent bottle 11 to flow out of the conduit 12.
On the basis of example 1, example 3: as shown in fig. 1: there are five reagent bottles 11, which are respectively a crystal violet reagent bottle 111, an iodine solution reagent bottle 112, a clear water reagent bottle 113, an ethanol reagent bottle 114 and a saffron reagent bottle 115.
Specifically, there are five reagent bottles 11, corresponding to five kinds of liquid, which are respectively a crystal violet reagent bottle 111, an iodine solution reagent bottle 112, a clear water reagent bottle 113, an ethanol reagent bottle 114 and a saffron reagent bottle 115; under the control of the reagent dripping controller 2, reagent dripping is carried out according to the sequence of a crystal violet reagent bottle 111, a clear water reagent bottle 113, an iodine solution reagent bottle 112, a clear water reagent bottle 113, an ethanol reagent bottle 114, a clear water reagent bottle 113, a yellow sand reagent bottle 115 and a clear water reagent bottle 113, and when crystal violet, iodine solution, ethanol and yellow sand are dripped, the platform angle rotating device 5 drives the slide card slot table 4 to rotate by an angle of 10-15 degrees; the reagent can slowly flow to the lower part, and the platform angle rotating device 5 is started to drive the slide clamping groove table 4 to rotate by 40-45 degrees when clear water is added every time, so that the reagent remained on the dropper 21 and the slide in the slide clamping groove table 4 can be conveniently and quickly cleaned and flushed out.
It should be understood that the arrangement of the inverted reagent bottle 11 can provide a pressurized pipeline from bottom to top under the gravity, thereby reducing the occurrence of tube blockage (especially crystal violet); meanwhile, the retention time of the dye solution on the slide is controlled by the inclination angle of the slide clamping groove table 4 and the washing time of clear water, so that the defects that the reagent of the existing spray dyeing device for vertically placing the slide is sprayed onto the slide and immediately drops, the dyeing is insufficient, the decoloration is incomplete and the like are overcome.
Preferably, the time consumption is about 20S when the crystal violet, the iodine solution and the sallow are dripped, about 30S when the ethanol is dripped, and about 40S when the clear water is dripped for washing.
Preferably, the upper end of each reagent bottle 11 is further provided with a normally closed opening for adding reagent after the reagent in the bottle is consumed.
Preferably, the sizes of the reagent bottles 11 are different according to the actual usage amount, so as to realize synchronous consumption of the reagents and facilitate uniform addition of the reagent dye solution, wherein the clear water reagent bottle 113 and the ethanol reagent bottle 114 are slightly larger.
On the basis of example 1, example 4: as shown in fig. 1: the automatic dripping and dyeing device for the gram dyeing further comprises a shell 7, the shell 7 is in a transparent box shape, and the shell 7 covers the reagent sample adding device 1.
Specifically, shell 7 is transparent box-packed cage cover in the outside of whole device, can prevent that the dye liquor from spattering outward, the polluted environment, and transparent setting simultaneously can be clear look over going on of inside flow.
Preferably, the lower end of the transparent box-packed shell 7 is formed by mutually connecting a plurality of surfaces of a hinged door, and the shell can be independently opened when needed, so that the operations of taking and adding objects are facilitated.
Example 5 on the basis of example 1: as shown in fig. 1: the reagent dripping controller 2 is internally provided with a plurality of openings which are communicated with the plurality of guide pipes 12 in a one-to-one correspondence manner, the plurality of openings are communicated with the dropper 21, and the reagent dripping controller 2 is also provided with a control switch for opening or closing a single opening.
Specifically, reagent dropwise add controller 2 controls the switching order and the time of inside a plurality of trompils, and then the different dye liquors of controlling in the different reagent bottles 11 how much that flow, and the dye liquor flows into burette 21 again after through the trompil, falls into the one end of slide draw-in groove platform 4 from burette 21 at last, and whole process design is reasonable, has reduced artifical loaded down with trivial details operation many times.
Preferably, the reagent drip controller 2 is prior art.
Example 6 on the basis of example 1: as shown in fig. 1: the connection part of the upper end of the guide pipe 12 and the reagent bottle 11 is also communicated with a vent pipe, and the vent pipe is communicated with the outside and the reagent bottle 11 and is used for balancing the pressure of the reagent bottle 11 and the outside.
Specifically, the breather pipe is used for balancing the atmospheric pressure with external in the reagent bottle 11, and then realizes the light outflow of liquid in the reagent bottle 11.
Preferably, a filter screen is further arranged in the vent pipe and used for filtering impurities in the air and preventing the reagent in the reagent bottle 11 from being influenced.
Example 7 on the basis of example 1: as shown in fig. 1: the platform angle rotating device 5 comprises a motor, a transmission gear set and a controller; the motor and the transmission gear set are both installed on the slide fixing device 3, the driving end of the motor is meshed with the input end of the transmission gear set, the output end of the transmission gear set is connected with and drives the slide clamping groove table 4 to rotate, and the controller is connected with and controls the motor to rotate.
Concretely, transmission gear group is the same with the reducing gear box function for alleviate the high-speed rotation of motor, make things convenient for the controller to regulate and control, under the control of controller, the motor rotates and drives transmission gear group, and transmission gear group drives 4 slopes to required angle of slide draw-in groove platforms, makes things convenient for the sample in the slide to be dyed evenly.
Preferably, limit switches are arranged on the transmission gear set and the slide clamping groove table 4, and when the slide clamping groove table 4 is inclined to a certain angle, the slide clamping groove table is not inclined, so that accidents are prevented.
On the basis of example 1, example 8: as shown in fig. 1: slide draw-in groove platform 4 is box-shaped structure, and inside lower extreme is equipped with the draw-in groove, and the low department of the slide draw-in groove platform 4 of slope still is equipped with the waste liquid water conservancy diversion mouth that is used for the waste liquid to flow out.
Specifically, slide draw-in groove platform 4 conveniently places the slide of taking the sample for box-like structure and dyes the experiment, and the dye liquor can not splash to the outside, and the slide joint is conveniently fixed in the draw-in groove simultaneously to whole slide draw-in groove platform 4 rotates the rocking that the in-process can not lead to the slide after dyeing.
Example 9 on the basis of example 1: as shown in fig. 1: the slide card slot table 4 further comprises a slide, and the slide can be detachably clamped in the slide card slot table 4.
Specifically, the joint can be dismantled to the slide in slide draw-in groove platform 4, and the convenience is after accomplishing at every turn, and the sample slide that carries out the renewal enters.
Preferably, one end of the slide slot station 4 is provided with a hinged open gate which is opened for replacement when a sample slide is replaced.
Example 10 on the basis of example 1: as shown in fig. 1: the automatic drip dyeing device for the gram dyeing further comprises a waste liquid box 6, and the waste liquid box 6 is installed below the waste liquid diversion port.
Specifically, the waste liquid box 6 is used for collecting waste liquid formed after each dyeing, so that the experimental environment is protected, and meanwhile, the waste liquid can be recycled through other processes.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims (10)

1. The utility model provides a blue dyeing automatic drip dyeing device which characterized in that includes:
the device comprises a reagent sample adding device (1), a reagent dripping controller (2), a slide fixing device (3), a slide clamping groove table (4) and a table angle rotating device (5);
the reagent sample adding device (1) comprises a plurality of reagent bottles (11) and a plurality of guide tubes (12) which are in one-to-one correspondence with the plurality of reagent bottles (11), each reagent bottle (11) is communicated with the reagent dripping controller (2) through the corresponding guide tube (12), and the reagent dripping controller (2) is arranged below the reagent sample adding device (1);
the glass slide fixing device (3) is of a plate-shaped flat plate structure and is fixed below the reagent dripping controller (2), the platform angle rotating device (5) is installed on the glass slide fixing device (3), and the glass slide clamping groove table (4) is obliquely and rotatably installed on the platform angle rotating device (5) in a manner that one end is higher and the other end is lower;
a dropper (21) is arranged below the reagent dripping controller (2), and liquid is dripped to a high position in the slide clamping groove table (4) below the dropper (21).
2. The automatic drip dyeing device for gram dyeing according to claim 1, characterized in that the automatic drip dyeing device for gram dyeing further comprises a reagent bottle holder, wherein a plurality of grooves are arranged on the reagent bottle holder, and a plurality of reagent bottles (11) are inversely placed in the plurality of grooves in a one-to-one correspondence manner.
3. The gram dyeing automatic drip device according to claim 1, characterized in that the reagent bottles (11) are five, namely a crystal violet reagent bottle (111), an iodine solution reagent bottle (112), a clear water reagent bottle (113), an ethanol reagent bottle (114) and a saffron reagent bottle (115).
4. The gram-dyeing automatic drop dyeing device according to claim 1, characterized in that the gram-dyeing automatic drop dyeing device further comprises a housing (7), the housing (7) is transparent box-shaped, and the housing (7) covers the reagent sample adding device (1).
5. The automatic drop dyeing device for the gram dyeing according to claim 1 is characterized in that a plurality of openings are formed in the reagent drop controller (2), the plurality of openings are communicated with the plurality of guide tubes (12) in a one-to-one correspondence manner, the plurality of openings are communicated with the dropper (21), and the reagent drop controller (2) is further provided with a control switch for opening or closing a single opening.
6. The automatic drip dyeing device for gram dyeing according to claim 1 is characterized in that the joint of the upper end of the conduit (12) and the reagent bottle (11) is further communicated with a vent pipe, and the vent pipe is communicated with the outside and the reagent bottle (11) and is used for balancing the pressure of the reagent bottle (11) and the outside.
7. The automatic drip dyeing device for gram dyeing according to claim 1, characterized in that the platform angle rotating device (5) comprises a motor, a transmission gear set and a controller; the glass slide fixing device is characterized in that the motor and the transmission gear set are installed on the glass slide fixing device (3), the driving end of the motor is meshed with the input end of the transmission gear set, the output end of the transmission gear set is connected with and drives the glass slide clamping groove table (4) to rotate, and the controller is connected with and controls the motor to rotate.
8. The automatic drop dyeing device for the gram dyeing according to claim 1, characterized in that the slide groove table (4) is of a box-shaped structure, a groove is arranged at the lower end of the inside of the slide groove table, and a waste liquid guide opening for the outflow of waste liquid is further arranged at the lower part of the inclined slide groove table (4).
9. The gram-staining automatic drip staining device according to claim 8, wherein the slide-channel table (4) further comprises a slide, which is detachably snapped into the slide-channel table (4).
10. The automatic drip dyeing device for gram dyeing according to claim 8, characterized in that it further comprises a waste liquid box (6), said waste liquid box (6) being installed below said waste liquid diversion opening.
CN202221033446.0U 2022-04-29 2022-04-29 Automatic drip dyeing device for gram dyeing Active CN217442965U (en)

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Application Number Priority Date Filing Date Title
CN202221033446.0U CN217442965U (en) 2022-04-29 2022-04-29 Automatic drip dyeing device for gram dyeing

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Application Number Priority Date Filing Date Title
CN202221033446.0U CN217442965U (en) 2022-04-29 2022-04-29 Automatic drip dyeing device for gram dyeing

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CN217442965U true CN217442965U (en) 2022-09-16

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116296704A (en) * 2022-12-05 2023-06-23 上海树突精密仪器有限公司 Living cell dyeing equipment

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116296704A (en) * 2022-12-05 2023-06-23 上海树突精密仪器有限公司 Living cell dyeing equipment
CN116296704B (en) * 2022-12-05 2023-12-12 上海树突精密仪器有限公司 Living cell dyeing equipment

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