CN210119387U

CN210119387U - Convenient dyeing box capable of uniformly dyeing samples in batches

Info

Publication number: CN210119387U
Application number: CN201920301977.5U
Authority: CN
Inventors: 李向宁; 陈思琦; 龚辉; 龙犇
Original assignee: Suzhou Institute Of Brain Space Information Huazhong University Of Science And Technology
Current assignee: Suzhou Institute Of Brain Space Information Huazhong University Of Science And Technology
Priority date: 2019-03-11
Filing date: 2019-03-11
Publication date: 2020-02-28
Anticipated expiration: 2029-03-11

Abstract

The utility model discloses a can evenly dye convenient dyeing box of sample in batches, including carrying the liquid box and the lid that closes mutually with it, detachably is provided with sample dyeing board on the liquid box, the upper surface of sample dyeing board is provided with a set of align to grid's sample and throws the hole, the sample is thrown the coaxial cylinder pipe that is provided with the same diameter under the hole, the bottom of cylinder pipe has through-hole and detachably and is provided with the rack, place the dyeing sample on the rack, the dyeing sample submergence in the dyeing liquid in the liquid box carries. The utility model discloses simple structure is exquisite, is equipped with a plurality of dyeing hole grooves in, can evenly dye the sample in batches, and the integrality of at utmost protection sample simultaneously to high efficiency.

Description

Convenient dyeing box capable of uniformly dyeing samples in batches

Technical Field

The utility model relates to an experimental apparatus equipment technical field specifically relates to a convenient dyeing box of evenly dyeing sample in batches.

Background

Since the refractive index of the constituent elements of the biological sample to light is low, such as carbon, hydrogen, oxygen, etc., and the atomic number of these elements is low, the ability to scatter electrons is weak, and therefore dyeing is required to enhance the contrast of the sample for better identification of the observation site.

With the invention and the general application of various staining methods in the field of biological science, the requirements on the treatment and staining quality of tissue sections are higher and higher. In the existing staining methods, the tissue samples need to be pretreated, such as rinsing, antigen retrieval, antigen-antibody reaction or incubation of biological samples. At present, most experimental processes deal with these steps by using a dropper to suck and add liquid in a 24-well plate for multiple times. The existing dyeing method has a plurality of problems: firstly, in the existing tissue staining, a sample sinks into the bottom of a pore plate, so that the phenomenon of uneven staining on two surfaces of the sample occurs, and the uniform marking of the whole sample cannot be realized. Secondly, the manual operation of sucking liquid and adding liquid by using a dropper is needed in the dyeing process, so that the sample is easily damaged due to improper operation, and the integrity of the tissue cannot be ensured. Finally, when the samples need to be processed in batches, repeated operations are needed, which is tedious, time-consuming and unstable.

SUMMERY OF THE UTILITY MODEL

The utility model aims at overcoming the not enough of prior art existence, provide a convenient dyeing box that can evenly dye the sample in batches.

The purpose of the utility model is realized through the following technical scheme:

the utility model provides a can evenly dye convenient dyeing box of sample in batches, includes the carrier liquid box and covers the lid that closes mutually with it, detachably is provided with sample dyeing board on the carrier liquid box, the upper surface of sample dyeing board is provided with a set of align to grid's sample and throws the hole, the sample is thrown and is put coaxial cylinder pipe that is provided with the same diameter under the hole, the bottom of cylinder pipe has through-hole and detachably and is provided with the rack, place the dyeing sample on the rack, the dyeing sample submergence in the dyeing liquid in the carrier liquid box.

Preferably, a cylindrical groove matched with the cylindrical pipe is arranged in the liquid carrying box, and the diameter of the cylindrical groove is not smaller than that of the cylindrical pipe, so that the cylindrical pipe can be sleeved in the cylindrical groove.

Preferably, the number of the cylindrical pipe and the cylindrical groove is two, and the cylindrical pipe and the cylindrical groove are arranged correspondingly.

Preferably, the bottom of cylinder pipe is provided with the screw thread that is used for supporting the rack is buckled, the screw thread buckle with be threaded connection between the inner wall of cylinder pipe.

Preferably, the thread fastener is provided with a driving groove, and is matched with a thread fastener wrench for installing the thread fastener in the cylindrical pipe, and the thread fastener wrench is provided with a corresponding bulge.

Preferably, the upper surface area of the sample staining plate is equal to the surface area of the upper port of the carrier fluid box.

Preferably, a sealing ring is arranged at the joint of the liquid carrying box and the box cover.

Preferably, the two sides of the bottom of the liquid carrying box and the box cover are respectively provided with a handle convenient to take.

The beneficial effects of the utility model are mainly embodied in that:

1. simple structure is exquisite, is equipped with a plurality of dyeing hole grooves in, can realize the batch dyeing of sample, and mutual independence between the dyeing hole groove has avoided influencing between the sample.

2. The detachable sample dyeing plate can realize rapid batch addition and replacement of the experimental liquid, save the experimental time and improve the efficiency of dyeing operation; the current situation that a pipette is used for sucking and adding liquid one by one manually is changed, and the integrity of a sample is protected to the greatest extent.

3. Through setting up the rack for the sample can unsettled abundant contact dye liquor, and the at utmost guarantees that the sample can two-sided even dyeing.

Drawings

The technical scheme of the utility model is further explained by combining the attached drawings as follows:

FIG. 1: the explosion schematic diagram of the embodiment of the utility model;

FIG. 2: the embodiment of the utility model is a schematic diagram of a box cover;

FIG. 3: the embodiment of the utility model provides a schematic diagram of sample dyeing plate;

FIG. 4: the embodiment of the utility model provides a schematic diagram of carrier liquid box;

FIG. 5: the embodiment of the utility model provides a top view of the thread button;

FIG. 6: the embodiment of the utility model provides a side view of thread button;

FIG. 7: the embodiment of the utility model provides a schematic diagram of a thread button wrench;

wherein: 1. a liquid carrying box; 11. a cylindrical groove; 12. an upper port; 13. a seal ring; 14. a handle; 2. a box cover; 3. a sample staining plate; 31. a sample inlet hole; 32. a cylindrical tube; 33. a net frame; 4. screwing; 41. a driving groove; 5. screwing a wrench; 51. and (4) protruding.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments shown in the drawings. However, these embodiments are not limited to the present invention, and structural, method, or functional changes made by those skilled in the art according to these embodiments are all included in the scope of the present invention.

In the description of the schemes, it should be noted that the terms "center", "upper", "lower", "left", "right", "front", "rear", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In the description of the embodiment, the operator is used as a reference, and the direction close to the operator is a proximal end, and the direction away from the operator is a distal end.

As shown in fig. 1 to 7, the utility model discloses a can evenly dye convenient dyeing box of sample in batches, including carrying liquid box 1 and lid 2 that closes mutually with it, in order to improve the leakproofness of dyeing box, avoid experiment pollution and dye liquor seepage, carry liquid box 1 with the junction of lid 2 is provided with sealing washer 13, sealing washer 13 is the rubber material, carry on the liquid box 1 with the junction of lid 2 is provided with the draw-in groove, is used for the joint sealing washer 13, makes sealing washer 13 sets firmly carry on the liquid box 1, in other embodiments, also can adopt the mode of splicing to make sealing washer 13 sets firmly carry on the liquid box 1.

In order to realize the quick change of the dye liquor box, a sample dyeing plate 3 is detachably arranged on the liquid carrying box 1, and the upper surface area of the sample dyeing plate 3 is equal to the surface area of the upper port 12 of the liquid carrying box 1. The upper surface of the sample dyeing plate 3 is provided with a group of uniformly arranged sample feeding holes 31, and cylindrical pipes 32 with the same diameter are coaxially arranged below the sample feeding holes 31.

In order to dye a sample conveniently, a cylindrical groove 11 matched with the cylindrical tube 32 is arranged in the liquid carrying box 1, and the diameter of the cylindrical groove 11 is not smaller than that of the cylindrical tube 32, so that the cylindrical tube 32 can be sleeved in the cylindrical groove 11 and can be lifted easily. The number of the cylindrical tube 32 and the cylindrical groove 11 is 24, and the cylindrical tube 32 and the cylindrical groove are arranged correspondingly to form independent dyeing hole grooves.

In order to enable the sample to be dyed uniformly on both sides, the bottom of the cylindrical tube 32 is provided with a through hole and a rack 33 is detachably arranged, and a dyed sample is placed on the rack 33 and is immersed in the dyeing liquid in the liquid carrying box 1. The net rack 33 is detachably arranged, so that after the net rack is used up, the net rack is detached and cleaned independently, and the defect that the experimental result is influenced due to the fact that the net rack is not cleaned in place under the fixed arrangement condition is avoided. Meanwhile, when the net rack 33 is damaged, the net rack can be replaced conveniently and timely.

In order to fix and support the net rack 33, the bottom of the cylindrical tube 32 is provided with a thread buckle 4 for supporting the net rack 33, the inner wall of the bottom of the cylindrical tube 32 is provided with spiral fine threads, and the thread buckle 4 is in threaded connection with the inner wall of the cylindrical tube 32.

In order to facilitate the mounting of the threaded buckle 4 to the bottom of the cylindrical tube 32, the threaded buckle 4 has a driving groove 41. For quick mounting of the threaded fastener 4, the threaded fastener 4 is fitted with a threaded fastener 5 for mounting it inside the cylindrical tube 32, the threaded fastener 5 having a projection 51 corresponding to the driving recess 41. Specifically, the thread buckle wrench 5 is clamped with the protrusion 51 thereof and the driving groove 41, and the thread buckle wrench 5 is rotated to enable the thread buckle 4 to rotate to be in threaded connection with the cylindrical pipe 32.

In order to facilitate opening and closing the box cover 2 and the dyeing box convenient to take, the two sides of the bottom of the liquid carrying box 1 and the box cover 2 are provided with handles 14 convenient to take.

For better observation experiment of being convenient for, the carrier liquid box 1, lid 2 and sample dyeing board 3 in the utility model are preferably made of transparent glass, and of course in other embodiments, other arbitrary suitable materials can be selected for use.

The working process of the utility model is as follows:

first, the net mount 33 is placed on the bottom of the cylindrical tube 32. Then, the screw 4 is screwed to the cylindrical pipe 32 using the screw wrench 5, and the net frame 33 is fixed. The sample staining plate 3 is then inserted in its entirety into the carrier 1, and the experiment is started: adding a proper amount of diluted experimental solution into the cylindrical groove 11 in the liquid carrying box 1 until the liquid level of the solution is higher than the net rack 33; putting a sample to be tested into the sample putting hole 31, and placing the sample on the net rack 33 to ensure that the sample is fully contacted with the dye liquor and is uniformly dyed; and covering the box cover 2 and the liquid carrying box 1, and placing the dyeing box on a shaking table to vibrate or incubate in an oven. When the liquid is changed and rinsed, the box cover 2 is opened, the sample dyeing plate 3 is lifted completely, the dye liquid in the cylindrical groove 31 is poured out, other experimental solutions are added, the sample dyeing plate 3 is embedded into the liquid carrying box 1 again, the box cover 2 is covered, and the next step is carried out.

The utility model discloses simple structure is exquisite, is equipped with a plurality of dyeing hole grooves in, can realize the batch dyeing of sample, and mutual independence has avoided influencing between the sample between the dyeing hole groove. The detachable sample dyeing plate can realize rapid batch addition and replacement of the experimental liquid, save the experimental time and improve the efficiency of dyeing operation; the current situation that a pipette is used for sucking and adding liquid one by one manually is changed, and the integrity of a sample is protected to the greatest extent. Through setting up the rack for the sample can unsettled abundant contact dye liquor, and the at utmost guarantees that the sample can two-sided even dyeing.

It should be understood that although the present description refers to embodiments, not every embodiment contains only a single technical solution, and such description is for clarity only, and those skilled in the art should make the description as a whole, and the technical solutions in the embodiments can also be combined appropriately to form other embodiments understood by those skilled in the art.

The above list of details is only for the practical implementation of the present invention, and they are not intended to limit the scope of the present invention, and all equivalent implementations or modifications that do not depart from the technical spirit of the present invention should be included in the scope of the present invention.

Claims

1. The utility model provides a but convenient dyeing box of batch homogeneous dyeing sample, includes carrier fluid box (1) and lid (2) that closes mutually with it, its characterized in that: the sample dyeing device is characterized in that a sample dyeing plate (3) is detachably arranged on the liquid carrying box (1), a group of uniformly-arranged sample feeding holes (31) are formed in the upper surface of the sample dyeing plate (3), cylindrical pipes (32) with the same diameter are coaxially arranged below the sample feeding holes (31), through holes are formed in the bottoms of the cylindrical pipes (32), a net rack (33) is detachably arranged on the bottoms of the cylindrical pipes (32), dyeing samples are placed on the net rack (33), and the dyeing samples are immersed in dyeing liquid in the liquid carrying box (1).

2. The convenient staining kit capable of uniformly staining samples in batches according to claim 1, wherein: the liquid carrying box (1) is internally provided with a cylindrical groove (11) matched with the cylindrical pipe (32), and the diameter of the cylindrical groove (11) is not smaller than that of the cylindrical pipe (32), so that the cylindrical pipe (32) can be sleeved in the cylindrical groove (11).

3. The convenient staining kit capable of uniformly staining samples in batches according to claim 2, wherein: the number of the cylindrical pipes (32) and the number of the cylindrical grooves (11) are 24, and the cylindrical pipes and the cylindrical grooves are arranged correspondingly.

4. The convenient staining kit capable of uniformly staining samples in batches according to claim 3, wherein: the bottom of cylinder pipe (32) is provided with and is used for supporting screw thread knot (4) of rack (33), screw thread knot (4) with be threaded connection between the inner wall of cylinder pipe (32).

5. The convenient staining kit capable of uniformly staining samples in batches according to claim 4, wherein the convenient staining kit comprises: the thread buckle (4) is provided with a driving groove (41) and is matched with a thread buckle wrench (5) used for installing the thread buckle (4) in the cylindrical pipe (32), and the thread buckle wrench (5) is provided with a corresponding protrusion (51).

6. The convenient staining kit capable of uniformly staining samples in batches according to claim 5, wherein the convenient staining kit comprises: the upper surface area of the sample staining plate (3) is equal to the surface area of the upper port (12) of the liquid carrying box (1).

7. The convenient staining kit capable of uniformly staining samples in batches according to claim 6, wherein the convenient staining kit comprises: and a sealing ring (13) is arranged at the joint of the liquid carrying box (1) and the box cover (2).

8. The convenient staining kit capable of uniformly staining samples in batches according to claim 7, wherein: handles (14) convenient to take are arranged on two sides of the bottom of the liquid carrying box (1) and the box cover (2).