CN220399072U - Transmission electron microscope sample batch negative dyeing device - Google Patents
Transmission electron microscope sample batch negative dyeing device Download PDFInfo
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- CN220399072U CN220399072U CN202321663883.5U CN202321663883U CN220399072U CN 220399072 U CN220399072 U CN 220399072U CN 202321663883 U CN202321663883 U CN 202321663883U CN 220399072 U CN220399072 U CN 220399072U
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- microscope sample
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- 238000004043 dyeing Methods 0.000 title claims abstract description 24
- 230000005540 biological transmission Effects 0.000 title claims abstract description 17
- 239000000178 monomer Substances 0.000 claims abstract description 28
- 238000010186 staining Methods 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims description 8
- 239000002390 adhesive tape Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 5
- 239000002699 waste material Substances 0.000 abstract description 2
- 239000000523 sample Substances 0.000 description 29
- 239000007788 liquid Substances 0.000 description 18
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 239000012472 biological sample Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 229910001385 heavy metal Inorganic materials 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000001803 electron scattering Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- PCTMTFRHKVHKIS-BMFZQQSSSA-N (1s,3r,4e,6e,8e,10e,12e,14e,16e,18s,19r,20r,21s,25r,27r,30r,31r,33s,35r,37s,38r)-3-[(2r,3s,4s,5s,6r)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-19,25,27,30,31,33,35,37-octahydroxy-18,20,21-trimethyl-23-oxo-22,39-dioxabicyclo[33.3.1]nonatriaconta-4,6,8,10 Chemical compound C1C=C2C[C@@H](OS(O)(=O)=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2.O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 PCTMTFRHKVHKIS-BMFZQQSSSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 238000006124 Pilkington process Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000012835 hanging drop method Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009980 pad dyeing Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a transmission electron microscope sample batch negative dyeing device, which relates to the field of electron microscope sample processing tools, and comprises a box body, wherein a dyeing pad is arranged in the box body, the upper surface of the dyeing pad is provided with a plurality of groove groups which are arranged in rows, each groove group comprises two rows of groove monomers, and the number of each groove monomer is multiple; the two sides of the box body are provided with clamping grooves at positions corresponding to the groove groups, the clamping grooves are clamped with net carrying frames, a plurality of net carrying frames are respectively arranged at the two sides of the net carrying frames, and the net carrying frames and the groove monomers are in one-to-one correspondence with each other. The utility model solves the problems of time and labor waste and low efficiency in the sample preparation process of a plurality of negative staining samples, and can process electron microscope samples in batches.
Description
Technical Field
The utility model relates to the field of electron microscope sample processing tools, in particular to a transmission electron microscope sample batch negative dyeing device.
Background
The negative staining sample preparation technology is to combine some matters with high electron density (such as heavy metal salt and the like) in the dye liquor with the background of the biological sample by utilizing the principle that heavy metal elements have stronger electron scattering capability than light elements forming the biological sample, so that the electron scattering capability of the background is improved, and the bright biological sample is reversely lined in the dark background.
There are various methods for preparing negative dye sample, such as hanging drop method, float method, carbon film-negative dye method, etc., and the common operation is to drop the sample on the carrier net or float the carrier net on the sample drop, absorb for 10s-1min, then clamp the carrier net with forceps, suck the redundant liquid with filter paper, drop the dye solution or float the carrier net on the dye solution, then clamp the carrier net with forceps, suck the redundant liquid with filter paper, thus preparing the negative dye sample. The whole operation process is suitable for preparing single samples rapidly, but is not suitable for negative staining of batch samples, and has the following disadvantages: firstly, the tweezers are required to be used repeatedly to clamp the carrying net, so that the supporting film on the carrying net is easily damaged, and the observation of an electron microscope is influenced; secondly, each sample needs to be dropwise added with the sample one by one, and redundant liquid is sucked by filter paper, so that the operation is tedious.
Disclosure of Invention
The utility model aims to provide a transmission electron microscope sample batch negative dyeing device which solves the problems of time and labor waste and low efficiency in sample preparation of a plurality of samples in the background technology.
The utility model adopts the following technical scheme:
the utility model relates to a transmission electron microscope sample batch negative dyeing device, which comprises a box body, wherein a dyeing pad is arranged in the box body, the upper surface of the dyeing pad is provided with a plurality of groove groups which are arranged in rows, each groove group comprises two rows of groove monomers, and the number of each groove monomer is multiple; the two sides of the box body are provided with clamping grooves at positions corresponding to the groove groups, the clamping grooves are clamped with net carrying frames, a plurality of net carrying frames are respectively arranged at the two sides of the net carrying frames, and the net carrying frames and the groove monomers are in one-to-one correspondence with each other.
Preferably, the groove monomer is designed as a hemispherical shape.
Preferably, the region where the groove group of the first half is located is a sample dripping region, and the region where the groove group of the second half is located is a dyeing region.
Preferably, letter marks are arranged on the left side of each row of groove monomers.
Preferably, a digital mark I is arranged above each groove monomer.
Preferably, the net carrying frame comprises a net carrying area, handles are respectively arranged at two ends of the net carrying area, transparent double-sided adhesive tapes are covered on the net carrying area, and edges of the net carrying area are adhered to a transverse line mark of the net carrying area through the double-sided adhesive tapes.
Preferably, a digital identifier two is arranged at the center of the network carrying area, and the digital identifier two corresponds to the network carrying area one by one.
Preferably, the two sides of the digital mark two are provided with transverse line marks.
Preferably, the box body is provided with a sealing box cover, and the inner wall of the box cover is provided with a sealing ring.
Compared with the prior art, the utility model has the beneficial technical effects that:
the utility model is suitable for negative dyeing sample preparation of batch samples, can realize micro and quantitative dyeing, saves reagents, and has the advantages of economy, convenience and high efficiency.
Drawings
The utility model is further described with reference to the following description of the drawings.
FIG. 1 is a schematic diagram of a negative staining apparatus for a transmission electron microscope sample in the present embodiment;
FIG. 2 is a top view of the dye pad of the present embodiment;
fig. 3 is a schematic structural diagram of a grid frame in the present embodiment;
FIG. 4 is an enlarged view of a portion of area A of FIG. 3;
FIG. 5 is a schematic diagram of the structure of the carrier web and the grooves in the present embodiment;
fig. 6 is a schematic structural view of the lid in the present embodiment.
Reference numerals illustrate: 1. a case body; 101. a clamping groove; 2. a dye pad; 201. a groove group; 201-1, groove monomer; 202. a sample dripping area; 203. a color zone; 204. letter identification; 205. the number marks I; 3. a grid carrier; 301. a network carrying area; 302. a handle; 303. a digital mark II; 304. a transversal mark; 4. carrying a net; 5. a box cover; 6. and a rubber sealing ring.
Detailed Description
In order to make the technical problems, technical schemes and beneficial effects to be solved more clear, the utility model is further described in detail below with reference to the accompanying drawings and embodiments.
As shown in fig. 1 and fig. 2, the embodiment discloses a negative staining device for a transmission electron microscope sample batch, which comprises a box body 1, wherein a staining pad 2 is arranged in the box body 1, the height of the staining pad 2 is lower than that of the box body 1, a plurality of groove groups 201 which are arranged in rows are arranged on the upper surface of the staining pad 2, the area where the groove groups 201 of the front half part are positioned is a sample dropping area 202, and the area where the groove groups 201 of the rear half part are positioned is a staining area 203.
Each groove group 201 comprises two rows of groove monomers 201-1, a plurality of groove monomers 201-1 are arranged in number, each groove monomer 201-1 is designed to be hemispherical, letter marks 204 are arranged on the left side of each groove monomer 201-1, ABCD … H is sequentially arranged from top to bottom, a digital mark 205 is sequentially arranged above each groove monomer 201-1, 1234 … is sequentially arranged from left to right, each groove monomer 201-1 can bear sample liquid drops or staining liquid with the volume of 10-20 microliters, and micro staining or quantitative liquid dropping can be achieved.
When in use, firstly, the sample liquid is dripped into the groove group 201 of the sample dripping area 202, and the dye liquid is dripped into the groove group 201 of the dyeing area 203.
The two sides of the box body 1 are provided with clamping grooves 101 at positions corresponding to the groove groups 201, the bottoms of the clamping grooves 101 and the upper surfaces of the dyeing pads 2 are positioned on the same plane, the clamping grooves 101 are clamped with grid loading frames 3, two sides of the grid loading frames 3 are respectively provided with a plurality of grid loading frames 4, and the grid loading frames 4 and the groove monomers 201-1 are in one-to-one correspondence with each other.
As shown in fig. 3 and fig. 4, the net carrier 3 includes a net carrier 301, handles 302 are respectively disposed at two ends of the net carrier 301, transparent double-sided adhesive tapes are covered on the net carrier 301, edges of the net carrier 4 are adhered to positions of transversal line marks 304 of the net carrier 301 through the double-sided adhesive tapes, AB marks are disposed at two sides of the net carrier 3 and used for distinguishing the net carrier 4 at left and right sides of the net carrier 301, a digital mark two 303 is disposed at a center of the net carrier 301, 1234 … 10 is disposed in sequence from left to right, transversal line marks 304 are disposed at two sides of the digital mark two 303, and the digital mark two 303 and the transversal line marks 304 correspond to the net carrier 4 one by one. The transverse line mark 304 can determine the contact width of the carrier net 4 and the adhesive surface, can effectively control the extremely trace adhesive quantity of each carrier net 4, keep the contact area consistent, avoid the damage of the supporting film or the adhesive pollution caused by the excessive contact of the carrier net 4 with the double-sided adhesive, simultaneously facilitate the more accurate matching with the groove monomer 201-1 of the dyeing pad 2, and the digital mark two 303 avoid the problem of confusion and disorder of batch processing samples.
As shown in fig. 5, after the sample liquid and the dye liquid are dropped into the corresponding groove monomers 201-1, the edges of the carrier net 4 are adhered to two sides of the carrier net frame 3, the carrier net frame 3 is placed in the clamping groove 101 of the box body 1, and at this time, the carrier net 4 is just placed on the upper surface of the groove monomers 201-1 and sequentially absorbs the sample liquid and the dye liquid, so that batch sample preparation of samples is realized.
As shown in fig. 6, a sealing box cover 5 is arranged on the box body 1, a rubber sealing ring 6 is arranged on the inner wall of the box cover 5, if a light-shielding environment needs to be sealed when sample liquid or dye liquid is adsorbed, the box cover 2 is covered on the box body 1, the rubber sealing ring 6 can be tightly connected with the outer wall of the box body 1, the light shielding and air isolation can be effectively sealed, and certain dye liquid and CO in the air are avoided 2 Heavy metal precipitation is generated after contact to pollute the carrying net.
The operation process of the utility model is as follows:
first, a pipette drops 10-20 microliters of sample into the well group 201 of the pad 2 drop zone 202 and drops 20 microliters of stain into the well group 201 of the pad zone 203.
And secondly, tearing off the transparent film on the upper layer of the double-sided adhesive tape on the net loading area 301 of the net loading frame 3, and adhering the net loading frame 4 on the net loading frame 3 by using special tweezers, wherein the edge of the net loading frame 4 is tangent to the transverse line mark 304 of the net loading frame 3.
The two ends of the hand-held net carrying frame 3 are placed in the clamping grooves 101 of the box bodies at two sides of the dyeing pad sample dripping area 202, so that the net carrying frame 4 just contacts with the surface of the sample liquid drops in the dyeing pad groove monomer 201-1, and when the light-shielding environment needs to be sealed, the box cover 5 can be covered on the box body 1. After adsorption for several seconds, the grid 3 is lifted up, and the filter paper sucks the redundant liquid one by one.
The net carrier 3 is placed in the clamping grooves 101 of the box body at two sides of the dyeing pad dyeing area 203, so that the net carrier 4 just contacts the surface of the dye solution drop in the dyeing pad groove monomer 201-1, and when the light-shielding environment needs to be sealed, the box cover 5 can be covered on the box body 1. After adsorption for several seconds, the grid 3 is lifted up, and the filter paper sucks the redundant liquid one by one.
Finally, the carrier web 4 is removed with special tweezers for electron microscopy.
It should be noted that the carrier net 4, the sample solution and the dye solution all belong to the prior art, and a person skilled in the art can implement the operation by himself.
The above embodiments are only illustrative of the preferred embodiments of the present utility model and are not intended to limit the scope of the present utility model, and various modifications and improvements made by those skilled in the art to the technical solutions of the present utility model should fall within the protection scope defined by the claims of the present utility model without departing from the design spirit of the present utility model.
Claims (9)
1. A transmission electron microscope sample batch negative staining device is characterized in that: the dyeing device comprises a box body (1), wherein a dyeing pad (2) is arranged in the box body (1), a plurality of groove groups (201) which are arranged in rows are arranged on the upper surface of the dyeing pad (2), each groove group (201) comprises two rows of groove monomers (201-1), and a plurality of groove monomers (201-1) are arranged in each row;
the two sides of the box body (1) are provided with clamping grooves (101) at positions corresponding to the groove groups (201), the clamping grooves (101) are clamped with a grid carrying frame (3), two sides of the grid carrying frame (3) are respectively provided with a plurality of grid carrying nets (4), and the grid carrying nets (4) and the groove monomers (201-1) are in one-to-one correspondence with each other.
2. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: the groove monomer (201-1) is designed to be a hemispherical shape.
3. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: the region where the groove group (201) of the front half part is located is a sample dripping region (202), and the region where the groove group (201) of the rear half part is located is a dyeing region (203).
4. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: letter marks (204) are arranged on the left side of each row of groove monomers (201-1).
5. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: and a digital mark I (205) is arranged above each groove monomer (201-1).
6. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: the net carrying frame (3) comprises a net carrying area (301), handles (302) are respectively arranged at two ends of the net carrying area (301), transparent double-sided adhesive tapes are covered on the net carrying area (301), and edges of the net carrying frame (4) are adhered to a transverse line mark (304) of the net carrying area (301) through the double-sided adhesive tapes.
7. The transmission electron microscope sample batch negative staining apparatus of claim 6, wherein: and a digital identifier two (303) is arranged at the center of the network carrying area (301), and the digital identifier two (303) corresponds to the network carrying area (4) one by one.
8. The transmission electron microscope sample batch negative staining apparatus of claim 7, wherein: and transverse line marks (304) are arranged on two sides of the digital mark II (303).
9. The transmission electron microscope sample batch negative staining apparatus of claim 1, wherein: the box body is provided with a sealing box cover (5), and the inner wall of the box cover (5) is provided with a sealing ring (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321663883.5U CN220399072U (en) | 2023-06-28 | 2023-06-28 | Transmission electron microscope sample batch negative dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321663883.5U CN220399072U (en) | 2023-06-28 | 2023-06-28 | Transmission electron microscope sample batch negative dyeing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220399072U true CN220399072U (en) | 2024-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321663883.5U Active CN220399072U (en) | 2023-06-28 | 2023-06-28 | Transmission electron microscope sample batch negative dyeing device |
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| Country | Link |
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| CN (1) | CN220399072U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117804872A (en) * | 2024-02-28 | 2024-04-02 | 广州智达实验室科技有限公司 | Electronic microscope sample preparation system and control method |
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2023
- 2023-06-28 CN CN202321663883.5U patent/CN220399072U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117804872A (en) * | 2024-02-28 | 2024-04-02 | 广州智达实验室科技有限公司 | Electronic microscope sample preparation system and control method |
| CN117804872B (en) * | 2024-02-28 | 2024-05-28 | 广州智达实验室科技有限公司 | Electronic microscope sample preparation system and control method |
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