CN213266545U - Multifunctional cell culture pore plate suitable for slide scanner - Google Patents
Multifunctional cell culture pore plate suitable for slide scanner Download PDFInfo
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- CN213266545U CN213266545U CN202022083496.7U CN202022083496U CN213266545U CN 213266545 U CN213266545 U CN 213266545U CN 202022083496 U CN202022083496 U CN 202022083496U CN 213266545 U CN213266545 U CN 213266545U
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Abstract
The utility model discloses a multifunctional cell culture pore plate suitable for a slide scanner, which comprises a plate body, a culture plate cover and a cover glass, wherein the culture plate cover is arranged at the top of the plate body; the outer wall and the bottom of the front end of the culture plate body are provided with notches in a penetrating manner, and the tops of the two sides of the culture plate body are connected with hangers; can be suitable for cell culture, more be favorable to doing various dyeings in the orifice plate than ordinary glass cover simultaneously, can avoid edge effect, can scan the film again behind the mounting, improve the efficiency of shooing, reduce the experiment deviation, can preserve the sample for a long time behind the mounting, and easy and simple to handle.
Description
Technical Field
The utility model relates to an ability cell culture orifice plate field, concretely relates to be suitable for multi-functional cell culture orifice plate of slide scanner.
Background
Cell staining is a common and indispensable experimental method in scientific research, for adherent cells, cells are cultured by using a 6-pore plate, a 24-pore plate and the like in a conventional mode, or cell climbing is carried out by using a clean cover glass, and then operation processes such as fixation, immunostaining and the like are carried out;
the multi-plate culture omits the operation related to slide climbing and is beneficial to the subsequent operation processes such as immunostaining, but has the following problems: the method comprises the following steps that firstly, an edge effect exists when the cells cultured by the multi-plate are subjected to immunostaining, namely, the edges of the multi-plate are often stained more deeply during staining; secondly, when a photo is taken, edge cells deform due to refractivity; different visual fields need to be manually moved during photographing, photographing takes longer time, particularly during photographing of a fluorescent picture, if the time interval of each group of photographing is longer, the fluorescence intensity may be weakened, the accuracy of an experiment is affected, manual photographing cannot guarantee that all visual fields are photographed, the visual fields are repeated, the problem of repeated counting exists in statistics, and the accuracy of data is affected; fourthly, after the cells are directly fixed and dyed in a multi-hole plate, the cells cannot be sealed, and the long-term storage of the specimen is difficult to realize; in a conventional cell staining experiment, unused culture wells of a multi-well plate are not fully utilized in many experiments, so that the waste of the multi-well plate is easily caused;
the cover glass slide-climbing method can well avoid the edge effect, can realize the long-term preservation of the specimen after the slide is sealed, is suitable for the slide scanner which is commonly used at present, replaces the traditional manual shooting, obviously improves the working efficiency and saves a large amount of working time; however, after a cover glass is put into a conventional culture dish for cell climbing, a culture medium is added, cells are cultured on the cover glass, after the cells are cultured, the cover glass needs to be clamped out by using tweezers, but the bottom of the culture dish is flat, and a microscopic cover glass is also flat, so that the cover glass needs to be quickly taken out under the condition of keeping the cover glass wet during experimental operation, when liquid exists, the cover glass is tightly attached to the plane of the bottom of the culture dish due to surface tension and is difficult to take out, even fragments occur, and unnecessary influence is caused on experimental results, in addition, when the cells are specially dyed, fixing and rinsing are involved, when a first antibody and a second antibody are required for incubation of dyeing liquid or immunostaining, a circle of hydrophobic region needs to be drawn around the cells of the climbing dish by using a grouping pen, so that the liquid is prevented from diffusing to the periphery during rinsing dyeing or incubating the antibodies, but the liquid still possibly overflows from the hydrophobic region when the slide shakes, the closed staining is incomplete, the experimental result is seriously influenced, the grouping pen is expensive, the experimental cost is increased, the section can be polluted during use, and in the subsequent mounting process, the periphery of the section is high for one circle, so that the cover glass can not be well attached to the glass slide, and the shooting effect can be influenced;
aiming at the respective defects of the current pore plate and the cover glass and the respective advantages of the set, a novel pore plate is designed, the pore plate is suitable for cell culture, various stains are suitable for being made in the pore plate, the reagent is prevented from diffusing to the periphery when a specimen is incubated, the serious deviation of an experimental result is avoided, the pore plate is directly pasted on a glass slide after being made, the scanning under a slide scanner is facilitated, the working efficiency is greatly improved, meanwhile, the waste is also avoided, and the long-time preservation of the specimen is facilitated.
SUMMERY OF THE UTILITY MODEL
The utility model aims to solve the technical problems that the prior culture pore plate and the prior cover glass are not suitable for cell culture, and can not simultaneously carry out various dyeing in the pore plate, reagents can diffuse to the periphery when incubating samples to cause serious deviation of experimental results, the slide glass after the experiment is finished is not convenient for scanning under a slide scanner, the work efficiency is greatly reduced, simultaneously waste is caused, and the samples are not beneficial to being preserved for a long time, the utility model provides a multifunctional cell culture pore plate suitable for the slide scanner, which is not only suitable for a common optical microscope and a fluorescence microscope, but also suitable for the multifunctional cell culture pore plate of the slide scanner, is suitable for cell culture, is more beneficial to carrying out various dyeing in the pore plate than the common cover glass, can avoid edge effect, can carry out scanning and shooting after the mounting, improves the efficiency and reduces the experimental deviation, the specimen can be preserved for a long time after being sealed, and the operation is simple and convenient; the additionally arranged notch design is convenient for easily taking and placing the cover glass from the notch by using tweezers, so that the design which is not beneficial to taking and placing the cover glass in the traditional cover glass culture is avoided, and meanwhile, the risk of breaking the glass slide in the taking and placing process is reduced; the hanging lugs on the two sides of the culture plate body are beneficial to taking the culture plate body and can be fixed on the external frame when a plurality of culture plate bodies are connected for use; adopt two kinds of culture holes or three two kinds of types of culture holes of cultivateing, avoid many culture holes hole number in the experiment too much to cause the waste, can also connect a plurality of culture plate bodies, facilitate the use for solve the defect that prior art leads to.
In order to solve the technical problem, the utility model provides a following technical scheme:
a multifunctional cell culture pore plate suitable for a slide scanner comprises a plate body, a culture plate cover and a cover glass, wherein the culture plate cover is arranged at the top of the plate body, a plurality of culture holes are formed in the plate body in a partitioning mode through the pore plate, the openings of the culture holes are upward, the culture plate body and the cover glass are sequentially placed in the culture holes from top to bottom, the culture plate cover is a cuboid with a downward opening, and the culture plate cover covers the culture plate body in a plate body box during cell culture;
the outer wall of the front end of the culture plate body is provided with a notch in a penetrating mode with the bottom, tweezers are convenient to clamp the bottom of the culture plate body from the junction of the bottom and the front end of the culture plate body, the bottom of the culture plate body is taken out of the notch and placed on the bottom of the culture plate body, meanwhile, dye liquor and various buffer solutions can enter the interior of the culture plate body when dyeing is facilitated, the tops of two sides of the culture plate body are connected with lugs, and the lugs are convenient to place the culture plate body.
The multifunctional cell culture pore plate suitable for the slide scanner is characterized in that the plate body is a cuboid with an upward opening.
The multifunctional cell culture pore plate suitable for the slide scanner is characterized in that any one corner of the outer wall of the plate body is a diagonal plane, and the diagonal plane forms a direction angle and plays a role in direction prompt.
The above multifunctional cell culture pore plate suitable for the slide scanner is characterized in that a tangent plane matched with the oblique tangent plane is arranged at one corner of the outer wall of the culture plate cover, so that the direction of the culture plate cover cannot be mistaken after the culture plate cover is marked on the plate body.
The multifunctional cell culture pore plate suitable for the slide scanner is characterized in that two or three culture pores are arranged, and in the practical application process, the culture pores can be independently applied or randomly combined by the two culture pores and the three culture pores, so that the waste of the pore plate is effectively avoided.
The multifunctional cell culture pore plate suitable for the slide scanner is characterized in that the culture plate body is a cube with an upward opening.
A multifunctional cell culture well plate suitable for a slide scanner as described above, wherein the surface of said cover glass has been coated.
The multifunctional cell culture well plate suitable for the slide scanner is characterized in that the cover glass is a square slide.
The procedure when staining cells using cell culture well plates was as follows:
s1: seeding cells onto the cover slips of a multi-functional well plate;
s2: intervening for a proper time to fix the cells, and carrying out various staining according to the experimental instruction;
s3: taking out the cover glass from the gap after dyeing, and reversely buckling one surface containing the cells on the glass slide for sealing;
s4: scanning, viewing and archiving are performed using a highly sensitive slide scanner.
According to above-mentioned the utility model relates to a be suitable for technical scheme that multi-functional cell culture orifice plate of slide scanner provided has following technological effect:
the utility model relates to a be suitable for slide scanner's multi-functional cell culture orifice plate not only is applicable to ordinary optical microscope and fluorescence microscope, is suitable for slide scanner's multi-functional cell culture orifice plate simultaneously, is suitable for cell culture, more is favorable to making various dyeings in the orifice plate than ordinary coverslip simultaneously, can avoid edge effect, can scan the film after the mounting again, improves the efficiency of shooing, reduces the experiment deviation, can preserve the sample for a long time after the mounting, and easy and simple to handle;
the additionally arranged notch design is convenient for easily taking and placing the cover glass from the notch by using tweezers, so that the design which is not beneficial to taking and placing the cover glass in the traditional cover glass culture is avoided, and meanwhile, the risk of breaking the glass slide in the taking and placing process is reduced;
the hanging lugs on the two sides of the culture plate body are beneficial to taking the culture plate body and can be fixed on the external frame when a plurality of culture plate bodies are connected for use;
two types of culture holes or three types of culture holes are adopted, waste caused by excessive holes of multiple culture holes in the test is avoided, and a plurality of culture plate bodies can be connected to facilitate use.
Drawings
FIG. 1 is a schematic diagram of a multi-functional cell culture well plate suitable for a slide scanner according to the present invention;
FIG. 2 is a schematic diagram of the structure of the plate body in the multifunctional cell culture well plate suitable for the slide scanner according to the present invention;
FIG. 3 is a schematic view of the structure of a culture plate body in a multifunctional cell culture well plate suitable for a slide scanner according to the present invention;
fig. 4 is a schematic view of the bottom structure of the culture plate body in the multifunctional cell culture well plate suitable for the slide scanner of the present invention.
Wherein the reference numbers are as follows:
Detailed Description
In order to make the technical means, the inventive features, the objectives, and the functions of the present invention easy to understand and understand, the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings.
Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve.
Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
The utility model provides a preferred embodiment provides a multi-functional cell culture orifice plate that is suitable for slide scanner, the purpose is not only suitable for ordinary optical microscope and fluorescence microscope, is suitable for the multi-functional cell culture orifice plate of slide scanner simultaneously, is suitable for cell culture, is more favorable to making various dyeings in the orifice plate than ordinary coverslip simultaneously, can avoid edge effect, can scan the film after the mounting again, improve the efficiency of shooing, reduce the experimental deviation, can preserve the sample for a long time after the mounting, and easy and simple to handle; the additionally arranged notch design is convenient for easily taking and placing the cover glass from the notch by using tweezers, so that the design which is not beneficial to taking and placing the cover glass in the traditional cover glass culture is avoided, and meanwhile, the risk of breaking the glass slide in the taking and placing process is reduced; the hanging lugs on the two sides of the culture plate body are beneficial to taking the culture plate body and can be fixed on the external frame when a plurality of culture plate bodies are connected for use; two types of culture holes or three types of culture holes are adopted, waste caused by excessive holes of multiple culture holes in the test is avoided, and a plurality of culture plate bodies can be connected to facilitate use.
As shown in fig. 1-4, a multifunctional cell culture well plate suitable for a slide scanner comprises a plate body 101, a culture plate body 102, a culture plate cover 103, and a cover glass 201, wherein the culture plate cover 103 is disposed on the top of the plate body 101, the plate body 101 is partitioned by a well plate 104 to form a plurality of culture wells 105, the culture wells 105 are opened upward, the culture plate body 102 and the cover glass 201 are sequentially placed in the culture wells 105 from top to bottom, the culture plate cover 103 is a cuboid with a downward opening, and the culture plate cover 103 covers the culture plate body 102 of the plate body 101 during cell culture;
the outer wall of the front end of the culture plate body 102 is provided with a notch 106 in a penetrating mode with the bottom, the bottom of the culture plate body 102 is clamped by tweezers conveniently through the notch 106 from the bottom of the culture plate body 102 and the front end junction, a cover glass 201 placed at the bottom of the culture plate body 102 is taken out along the notch 106, meanwhile, dye solution and various buffer solutions can enter the culture plate body 102 when dyeing is facilitated, the tops of two sides of the culture plate body 102 are connected with hanging lugs 107, and the culture plate body 102 can be placed conveniently through the hanging lugs 107.
The plate body 101 is a rectangular parallelepiped with an upward opening.
Any one corner of the outer wall of the plate body 101 is a chamfer 108, and the chamfer 108 forms a direction angle to play a role in direction prompt.
Wherein, a tangent plane 109 matched with the inclined tangent plane 108 is arranged at one corner of the outer wall of the culture plate cover 103, so that the direction cannot be wrong after the culture plate cover 103 is covered on the plate body 101 after the culture plate cover 103 is marked.
Two or three culture holes 105 are arranged, and in the practical application process, the culture holes 105 can be independently applied or randomly combined with the three culture holes 105, so that the waste of the hole plate 104 is effectively avoided.
The culture plate body 102 is a cube with an upward opening.
Wherein the surface of the cover glass 201 has been coated.
Wherein the cover glass 201 is a square glass slide.
The steps when cell staining was performed using cell culture well 105 plates 104 were as follows:
s1: planting cells onto the coverslip 201 of the multi-functional well plate 104;
s2: intervening for a proper time to fix the cells, and carrying out various staining according to the experimental instruction;
s3: taking out the cover glass 201 from the notch 106 after dyeing, and reversely buckling the surface containing the cells on the glass slide for sealing;
s4: scanning, viewing and archiving are performed using a highly sensitive slide scanner.
To sum up, the utility model discloses a multi-functional cell culture orifice plate suitable for slide scanner not only is applicable to ordinary optical microscope and fluorescence microscope, is suitable for the multi-functional cell culture orifice plate of slide scanner simultaneously, is suitable for cell culture, is more favorable to making various dyeings in the orifice plate than ordinary coverslip simultaneously, can avoid edge effect, can scan the piece after the mounting again, improves the efficiency of shooing, reduces the experiment deviation, can preserve the sample for a long time after the mounting, and easy and simple to handle; the additionally arranged notch design is convenient for easily taking and placing the cover glass from the notch by using tweezers, so that the design which is not beneficial to taking and placing the cover glass in the traditional cover glass culture is avoided, and meanwhile, the risk of breaking the glass slide in the taking and placing process is reduced; the hanging lugs on the two sides of the culture plate body are beneficial to taking the culture plate body and can be fixed on the external frame when a plurality of culture plate bodies are connected for use; two types of culture holes or three types of culture holes are adopted, waste caused by excessive holes of multiple culture holes in the test is avoided, and a plurality of culture plate bodies can be connected to facilitate use.
The above description has been made of specific embodiments of the present invention. It is to be understood that the invention is not limited to the particular embodiments described above, and that devices and structures not described in detail are understood to be implemented in a manner common in the art; various changes or modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the invention.
Claims (8)
1. A multifunctional cell culture pore plate suitable for a slide scanner is characterized by comprising a plate body, a culture plate cover and a cover glass, wherein the culture plate cover is arranged at the top of the plate body;
the outer wall of the front end of the culture plate body is provided with a notch in a penetrating mode with the bottom, and the tops of the two sides of the culture plate body are connected with hanging lugs.
2. The multifunctional cell culture well plate for a slide scanner according to claim 1, wherein the plate body is a rectangular parallelepiped with an upward opening.
3. The multifunctional cell culture well plate for a slide scanner of claim 2, wherein any one corner of the outer wall of the plate body is beveled.
4. The multifunctional cell culture well plate for slide scanner of claim 3, wherein a corner of the outer wall of the plate cover is provided with a cut surface matching the beveled surface.
5. A multifunctional cell culture well plate suitable for a slide scanner according to any of claims 1-4, wherein there are two or three culture wells.
6. The multifunctional cell culture well plate for a slide scanner of claim 5, wherein said culture plate body is a square with an upward opening.
7. A multifunctional cell culture well plate suitable for a slide scanner according to claim 1 or 6, wherein the surface of the coverslip has been coated.
8. A multifunctional cell culture well plate for a slide scanner according to claim 7, wherein said cover slide is a square slide.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022083496.7U CN213266545U (en) | 2020-09-22 | 2020-09-22 | Multifunctional cell culture pore plate suitable for slide scanner |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022083496.7U CN213266545U (en) | 2020-09-22 | 2020-09-22 | Multifunctional cell culture pore plate suitable for slide scanner |
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| Publication Number | Publication Date |
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| CN213266545U true CN213266545U (en) | 2021-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022083496.7U Active CN213266545U (en) | 2020-09-22 | 2020-09-22 | Multifunctional cell culture pore plate suitable for slide scanner |
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