CN210465070U

CN210465070U - Dyeing plate for colloidal gold immunity labeling

Info

Publication number: CN210465070U
Application number: CN201920930959.3U
Authority: CN
Inventors: 聂佩显; 高艳; 路超; 王来平; 韩雪平
Original assignee: Shandong Institute of Pomology
Current assignee: Shandong Institute of Pomology
Priority date: 2019-06-20
Filing date: 2019-06-20
Publication date: 2020-05-05
Anticipated expiration: 2029-06-20

Abstract

The utility model provides a dyeing plate for colloidal gold immunity marking, which comprises a bottom plate and a surface film covered on the bottom plate and having water repellent function; a plurality of deep holes are formed in the vertical direction of the upper surface of the bottom plate; the deep holes are arranged in m rows and n rows; the deep hole is a through hole with one end opened or penetrating through the bottom plate. The pellicle may be a waxed paper or a soft and pliable inert plastic film. The dyeing plate for the colloidal gold immunity mark of the utility model has small volume and convenient operation; can be matched with a pipettor to process more samples at one time, thereby saving time and labor; the dosage of various reagents is less, only a few microliters is needed, and the dyeing cost is low; the surface film is low in cost and disposable, and vessels do not need to be washed, so that labor and time are saved. The method solves the problems that the reagent dosage is large, the cost is high, the sample is easy to damage, and the vessels need to be cleaned, which consumes manpower and time in the existing colloidal gold immune marking dyeing process, and can be widely used in the colloidal gold immune marking dyeing test.

Description

Dyeing plate for colloidal gold immunity labeling

Technical Field

The utility model belongs to the experimental device field, concretely relates to dyeing plate that is arranged in resin embedding sample immune colloidal gold mark technique.

Background

The colloidal gold is hydrosol of chloroauric acid, can be polymerized into gold particles with specific sizes under the action of a reducing agent, and has the physical characteristics of high electron density and the like. The immune colloidal gold technology is an immune labeling technology which takes colloidal gold as a tracer marker, utilizes antigen-antibody reaction and observes a test result by means of an electron microscope.

With the more mature polyclonal antibody and monoclonal antibody technology, the immune colloidal gold technology is widely applied. The immune colloidal gold technique is generally divided into three steps: embedding a sample, performing immunostaining and observing by using an electron microscope. In staining a resin-embedded sample, it is currently common practice to stain the sample on the carrier net with different liquid reagents for antibody-antigen reaction, and the general immunostaining procedure is as follows: sodium periodate etching, HCl etching, sealing nonspecific sites, adding a primary anti-incubation treatment, cleaning, adding a gold-labeled secondary antibody for incubation treatment, rinsing, uranyl acetate dyeing and rinsing.

At present, immunostaining is usually performed in a culture dish or an eppendorf tube, the operating reagent dosage in the culture dish is large, and when the eppendorf tube is used for operation, although the reagent dosage is reduced, a net needs to be transferred by tweezers or other tools in the operating process, so that a sample is easy to damage. When a culture dish or an eppendorf tube is adopted for dyeing, the dosage of the added colloidal gold, primary antibody and secondary antibody reagents is different from dozens of milliliters to dozens of milliliters, and the dosage is upgraded. The reagents have high price, large consumption and high cost; the used utensils need to be cleaned, which takes much time; in addition, tweezers or other instruments are needed to repeatedly transfer the carrier net in the operation process, so that the sample is easily damaged. Therefore, there is a need to develop a new staining apparatus for the staining process of the colloidal gold immunolabeling.

Disclosure of Invention

To big, with high costs of present colloidal gold immunity mark dyeing in-process reagent quantity, sample fragile, household utensils need wash the problem that consumes manpower and time, the utility model provides a colloidal gold immunity mark is with dyeing board can once only handle a large amount of samples, and the reagent quantity is very few, for disposable articles for use, only need change low-cost epidermis, need not to wash labour saving and time saving.

In order to achieve the above purpose, the utility model adopts the following technical scheme.

A staining plate for colloidal gold immunity marking comprises a bottom plate and a surface film covered on the bottom plate and having water repellent effect; a plurality of deep holes are formed in the vertical direction of the upper surface of the bottom plate; the deep holes are arranged in m rows and n rows, and m and n are positive integers greater than 1; the deep hole is a through hole with one end opened or penetrating through the bottom plate. Preferably, the deep holes on the bottom plate are 12 columns and 8 rows or 24 columns and 16 rows.

The pellicle may be a waxed paper or a soft and pliable inert plastic film, such as PARAFILM

Sealing films or other flexible, plastic films that do not react with the reagents.

Preferably, different rows or columns on the base plate have indicia to distinguish between different wells.

Preferably, when the deep hole on the base plate is a through hole, the surface film is a soft and plastic inert plastic film. More preferably, a bottom film with good air permeability is attached to the lower surface of the bottom plate, and the bottom film covers the corresponding area of the deep hole. Furthermore, the dyeing plate also comprises a funnel matched with the bottom plate, and the section of the opening is larger than the area corresponding to the deep hole. In order to be matched with the funnel for use, four sides of the lower surface of the bottom plate are respectively provided with a groove which are communicated with each other to form a rectangular groove; the rectangular groove is adaptive to the section of the opening of the funnel.

Preferably, the dyeing plate is further provided with an upper cover, and the upper cover is not in contact with the upper surface of the bottom plate.

Colloidal gold is dyeing board for immunological marking when using, will refuse the water pellicle and spread on the bottom plate, with multichannel pipetting gun with liquid dropwise add on the pellicle that the deep hole corresponds, will carry the net to put and float in liquid, only need use the pipetting gun when changing liquid with liquid absorption remove can, need not adopt tweezers to carry the net to shift to in the new reagent solution. The pellicle after the completion of the immunostaining operation is not recycled and can be directly discarded, and the process of trying to brush utensils is omitted. The matched funnel can form a certain degree of depression on the surface film, and the bottom film can prevent the suction force from being too strong and the depression from being too deep; the formed recess can prevent the situation that liquid drops roll and the like possibly caused by moving the dyeing plate in the operation process, and is convenient for processing a large number of samples at one time.

The utility model has the advantages of it is following:

the dyeing plate for the colloidal gold immunity mark of the utility model has small volume and convenient operation; can be matched with a pipettor to process more samples at one time, thereby saving time and labor; the dosage of various reagents is less, only a few microliters is needed, and the dyeing cost is low; the surface film is low in cost and disposable, and vessels do not need to be washed, so that labor and time are saved. The method solves the problems that the reagent dosage is large, the cost is high, the sample is easy to damage, and the vessels need to be cleaned, which consumes manpower and time in the existing colloidal gold immune marking dyeing process, and can be widely used in the colloidal gold immune marking dyeing test.

Drawings

FIG. 1 is a schematic view showing the structure of a staining plate for colloidal gold immunolabeling in example 1;

FIG. 2 is a schematic view of the bottom plate in the example;

FIG. 3 is a schematic view showing the structure of a staining plate for colloidal gold immunolabeling in example 2.

Detailed Description

The present invention will be further described with reference to the following examples and drawings, but the present invention is not limited to the following examples.

Example 1

A staining plate for colloidal gold immunolabeling, as shown in figure 1 and figure 2, comprises a bottom plate (1) made of hard plastic material, wax paper (2) covering the bottom plate (1) and an upper cover (3); 12 rows of deep holes (4) with 8 rows are formed in the vertical direction of the upper surface of the bottom plate (1); one end of the deep hole (4) is opened; the front end of the upper surface of the bottom plate (1) is engraved with column marks of numerical marks, and the left end is provided with row marks of letter marks for distinguishing different holes; the height of the upper cover (3) is greater than that of the bottom plate (1).

During the use, lay wax paper (2) on bottom plate (1), absorb reagent solution to wax paper (2) surface that deep hole (4) correspond with multichannel pipettor, cover upper cover (3), wait the time to reach the back, uncap and remove liquid with multichannel pipettor to trade other reagent solutions, so relapse until all immunolabelling and dyeing are accomplished.

Example 2

A staining plate for colloidal gold immunolabeling, as shown in fig. 2 and 3, comprises a base plate (1) made of hard plastic, and PARAFILM coated on the base plate (1)

A sealing film (2), a bottom film (6) which is adhered to the bottom plate (1) and is made of non-woven fabrics, an upper cover (3) and a funnel (5); a gap is reserved between the lower surface of the upper cover (3) and the upper surface of the bottom plate (1) and is not contacted; the upper surface of the bottom plate (1) is vertically provided with 12 rows and 8 rows of through holes (4); the front end of the upper surface of the bottom plate (1) is engraved with column marks of numerical marks, and the left end is provided with row marks of letter marks for distinguishing different holes; the bottom film (6) covers the corresponding area of the through hole (4); four sides of the lower surface of the bottom plate are respectively provided with a groove (7) which are communicated with each other to form a rectangular groove. The shape and the wall thickness of the opening section of the funnel (5) are adapted to the rectangular groove.

When the air-permeable bottom plate is used, the sealing film (2) is coated on the upper surface of the bottom plate (1), the film surface is flat and free of wrinkles, the upper end of the through hole (4) has certain air tightness, the funnel (5) is connected with a vacuum pump through a pipeline, then the funnel is clamped in the groove (7), air is extracted through the air-permeable bottom film (6), and the sealing film (2) at the through hole (5) of the bottom plate (1) is sunken by 1-2 mm; then put into and carry the net and absorb reagent solution to sealing membrane (2) surface that through-hole (4) correspond with multichannel pipettor, cover upper cover (3), after the time arrives, uncap and remove liquid with multichannel pipettor to trade other reagent solutions, so relapse until all immunolabeling dyeing is accomplished, tear the sealing membrane after the completion and remove and abandon.

Claims

1. A dyeing plate for colloidal gold immunity marking is characterized by comprising a bottom plate and a surface film which covers the bottom plate and has water repellency; a plurality of deep holes are formed in the vertical direction of the upper surface of the bottom plate; the deep holes are arranged in m columns and n rows.

2. The dyed plate for gold colloidal immunological labeling according to claim 1, wherein said deep hole is a through hole having an opening at one end or penetrating through the bottom plate.

3. The staining plate for gold colloidal immunological labeling according to claim 1, wherein the wells of the bottom plate are 12 columns and 8 rows or 24 columns and 16 rows.

4. The staining plate for gold colloidal immunological labeling according to claim 1, wherein the bottom plate has labels in different rows or columns.

5. The dyed plate for gold colloidal immunological labeling according to claim 1, wherein said top film is a wax paper or a flexible and moldable inert plastic film.

6. The dyed plate for gold colloidal immunity labeling according to claim 1, wherein the top film is a flexible and moldable inert plastic film when the deep hole is a through hole in the bottom plate.

7. The dyed plate for a colloidal gold immunological marker according to claim 6, wherein a gas-permeable base film is attached to a lower surface of the base plate, and the base film covers a region corresponding to the deep hole.

8. A stained plate for colloidal gold immunolabeling according to any one of claims 6 or 7, wherein the stained plate further comprises a funnel fitted with the bottom plate, and the cross section of the opening is larger than the area corresponding to the deep hole.

9. The staining plate for gold colloidal immunity labeling according to claim 8, wherein four sides of the bottom surface of the bottom plate are respectively provided with a groove, which are communicated with each other to form a rectangular groove; the rectangular groove is adaptive to the section of the opening of the funnel.

10. The staining plate for gold colloidal immunological labeling according to claim 1, further comprising an upper cover, wherein the upper cover is not in contact with the upper surface of the base plate.