CN108913585A - A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish - Google Patents
A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish Download PDFInfo
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- CN108913585A CN108913585A CN201810988992.1A CN201810988992A CN108913585A CN 108913585 A CN108913585 A CN 108913585A CN 201810988992 A CN201810988992 A CN 201810988992A CN 108913585 A CN108913585 A CN 108913585A
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- wall
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- downside wall
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
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Abstract
The invention discloses a kind of extrusion type leakproofs easily to mark visual criss-cross method surface sampling culture dish, the culture dish includes ware bottom and ware lid, ware bottom is equipped in the second buckle and the first buckle for stablizing embedded structure, embedded structure can unclamp when stressed, relatively move movable ware downside wall with the first ware downside wall.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, mark zone can make markers work more efficient, and observation window can make the observation of minute colony more convenient, and grid line can simplify the counting process of bacterium colony.
Description
Technical field
The present invention relates to a kind of experiment equipment, especially a kind of extrusion type leakproof easily marks visual criss-cross method surface to sample
Culture dish.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware
The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab
Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face
To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity
Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism
Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages
It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold
Easily cause the waste of human resources.Existing culture dish is mostly glass material, need to could be marked with permanent pen, but oiliness
The person's handwriting of marking pen need to could be wiped with special organic solvent, therefore when multiple culture dishes are reused, the not side of label
Just it be easy to cause and obscures.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to use microscope
By bacterium colony amplification by observe bacterium colony it is accurate in the form of, need to open in culture dish during magnify bacterium colony, this possibility
Pollution or the leakage of harmful culture can be brought.When the colony counts for the target culture that the culture medium in culture dish is grown are huge
When big, the counting process of bacterium colony is then comparatively laborious.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide a kind of extrusion type leakproof that structure is simple and convenient to operate is easy
Mark visual criss-cross method surface sampling culture dish.The implementation of the culture dish can simplify the sampling of body surface microorganism
Journey improves the accuracy of test result, improves working efficiency.
Visual criss-cross method surface sampling culture dish is easily marked for extrusion type leakproof, to reach the purpose, the present invention is adopted
Technical solution is：A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish, including ware bottom and relaxation
The ware lid being covered on ware bottom, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware holded up from tegmental wall
Lid side wall, the ware bottom include bottom wall and at least one from bottom wall around the first ware downside wall for holding up and at least one
Movable ware downside wall of the parallel sleeve on the outside of the first ware downside wall；The first ware downside wall is equipped at least one circle second
Buckle, second snap collar was around described first ware downside wall one week, the circle plane of second buckle and the bottom wall
Plane is parallel；The movable ware downside wall is equipped at least one the first buckle of circle, and first snap collar is around the movable ware bottom
Side wall one week, the circle plane of first buckle was parallel with the plane of the bottom wall；Described first buckle and described the
Two buckles can be closely fitted into, to maintain to be in above the first ware downside wall without activity ware downside wall described under external force
Technical characteristic；When along from movable ware downside wall to the direction of the first ware downside wall, applying force to the movable ware bottom
When side wall, the embedded structure of first buckle and second buckle can unclamp, and movable ware downside wall can be along the at this time
The direction of one ware downside wall moves in parallel；The diameter of the ware lid side wall is greater than the first ware downside wall；The bottom wall it is straight
Diameter is greater than the tegmental wall；The height of the activity ware downside wall and the sum of the height of the first ware downside wall, are less than the ware
The height of lid side wall；The culture dish further includes that at least one surround the second ware downside wall holded up, the second ware bottom from bottom wall
The height of side wall is less than the ware lid side wall, and the diameter of the second ware downside wall is greater than ware lid side wall, the first ware downside wall and the
Two ware downside walls are located at the same side of the bottom wall；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the mark
Note area is formed by wall surfaces through frosted processing；At least one transparent observation window, the observation are additionally provided in the tegmental wall
Window is in convex lens shape, and is thickeied by the tegmental wall center and formed；In the bottom wall, equipped with vertical and horizontal orthogonal crossover
Bottom wall is divided into the region of several squares or approximating square, the square area by grid line, the grid line
Area equation.
Compared with the prior art, the beneficial effects of the invention are as follows：The structure of the culture dish is simple and convenient to operate, can be with
The process for simplifying sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, mention simultaneously
High test result accuracy and working efficiency.In addition, the second ware downside wall can prevent culture medium from overflowing from ware bottom, mark zone can make
Markers work is more efficient, and observation window can make the observation of minute colony more convenient, and grid line can simplify the counting process of bacterium colony.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description
Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field
For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram that extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish；Fig. 2 is provided at
The schematic diagram of the grid line of the bottom wall；In figure：
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 6 be the second ware downside wall, and 7 be the first ware bottom side
Wall, 71 be movable ware downside wall, and 72 be the first buckle, and 73 be the second buckle, and 13 be gap, and 20 be frosted mark zone, and 21 be to see
Window is examined, 23 be grid line.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention
The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on
Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish
Shape preferably selects circle, and still, those skilled in the art should be understood that it is suitable for such as oval
Shape, rectangle or other shapes suitable for culture dish.
The invention discloses a kind of extrusion type leakproofs easily to mark visual criss-cross method surface sampling culture dish, including ware bottom 2
The ware lid 1 being covered on ware bottom 2 with relaxation, the ware lid 1 includes tegmental wall 3 and at least one surround the ware holded up from tegmental wall 3
Lid side wall 5, the ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4, and at least
Movable ware downside wall 71 of one parallel sleeve in 7 outside of the first ware downside wall；The diameter of the ware lid side wall 5 is greater than described
Movable ware downside wall 71；The bottom wall 4 is identical as the shape of the tegmental wall 3.The first ware downside wall 7 is equipped at least one circle
Second buckle 73, second buckle 73 around described first ware downside wall 7 one weeks, the circle plane of second buckle 73 and
The plane of the bottom wall 4 is parallel；The movable ware downside wall 71 is equipped at least one the first buckle 72 of circle, first buckle 72
Around described movable ware downside wall 71 1 weeks, the circle plane of first buckle 72 was parallel with the plane of the bottom wall 4；It is described
The first buckle 72 and described second buckle 73 can closely be fitted into, to maintain without activity ware downside wall described under external force
71 technical characteristics in 7 top of the first ware downside wall；When along certainly movable 71 to the first ware downside wall 7 of ware downside wall
Direction, when applying force to the movable ware downside wall 71, the chimeric knot of first buckle 72 and second buckle 73
Structure can unclamp, and movable ware downside wall 71 can be moved in parallel along the direction of the first ware downside wall 7 at this time.Features described above has determined
The extrusion type leakproof easily marks the basic structure of visual criss-cross method surface sampling culture dish, wherein the first ware downside wall 7, work
The space that dynamic ware downside wall 71 and bottom wall 4 are formed is as culture inner cavity, when enough agar mediums are injected in culture inner cavity,
After the described culture medium solidification, the movable ware downside wall 71 is applied force to, makes movable ware downside wall 71 along first
The direction of ware downside wall 7 moves in parallel, until the plane of movable ware downside wall 71 is lower than the plane of the agar medium, uses
When the agar medium plane is directly covered in object under test surface, that is, can reach the mesh of sampling microorganisms from surface of object
, it avoids and carries out the tedious steps eluted again after microorganism acquisition using cotton swab or filter paper, greatly simplify object
The process of surface microorganism sampling, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, it improves simultaneously
Test result accuracy and working efficiency.
In the embodiment that the extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish, the bottom wall 4
Diameter be greater than the tegmental wall 3 diameter, the setting of this feature, can limit when ware lid 1 and ware bottom 2 fastening when, ware lid side wall 5
Bottom wall 4 can only be supported in.In addition, the height of the activity ware downside wall 71 and the sum of the height of the first ware downside wall 7, small
In the height of the ware lid side wall 5, this feature can limit activity ware downside wall 71 and tegmental wall 3 when ware lid 1 and the fastening of ware bottom 2
Between there are certain gaps, prevent the agar medium from contacting with tegmental wall 3.
The culture dish further includes that at least one surround the second ware downside wall 6 holded up, the first ware downside wall from bottom wall 4
7 and second ware downside wall 6 be located at the same side of the bottom wall 4.The diameter of the ware lid side wall 5 is greater than the first ware downside wall 7
Diameter, but less than the diameter of the second ware downside wall 6, this feature is limited when the ware lid 1 and the ware bottom 2 fastening, ware lid
Side wall 5 is fastened on the centre of the first ware downside wall 7 and the second ware downside wall 6 just.In addition, the height of the second ware downside wall 6 is small
In the ware lid side wall 5, this feature can prevent the second ware downside wall 6 from hindering the unlatching of the ware lid 1 with ware bottom 2
Or it fastens.First ware downside wall 7 and the second ware downside wall 6 and bottom wall 4 form gap 13, the gap 13 can collect to
The agar medium of spill and leakage, prevents the culture medium from overflowing from ware bottom 2 when injecting agar medium in culture dish.
In the outer surface of the tegmental wall 3, it is equipped at least one mark zone 20, the mark zone 20 is passed through by 3 surface of tegmental wall
Frosted processing is formed；The rough surface of the mark zone 20, common pencil, wax crayon, white board marker etc. can be in mark zones 20
Apparent person's handwriting is formed, and the person's handwriting is washed with water and can easily be removed, and keeps the markers work of the culture dish more square
Just efficiently.
The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by
The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture
The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony
The leakage of dye or harmful culture.
In the bottom wall 4, the grid line 23 equipped with vertical and horizontal orthogonal crossover, bottom wall 4 is divided by the grid line 23
The region of several squares or approximating square, the area equation of the square area；When the culture medium institute in culture dish
When the colony counts of the target culture of growth are huge, the bacterium colony count results in a square area can be used, multiplied by phase
The multiple answered（In the embodiment shown in Figure 2, the side length of the square area is 1 centimetre, agar medium in ware bottom 2
Diameter is 10 centimetres, then its area is 78.5 square centimeters, so multiple is 78.5）To indicate all clump counts of culture dish,
Therefore the counting process of bacterium colony is simplified.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute
The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally
Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair
Bright protection scope.
Claims (1)
1. a kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish, including ware bottom and relaxation to be covered in ware bottom
On ware lid, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, described
Ware bottom include bottom wall and at least one from bottom wall around the first ware downside wall for holding up and at least one parallel sleeve described
Movable ware downside wall on the outside of first ware downside wall；The first ware downside wall is equipped at least one the second buckle of circle, and described the
For two snap collars around described first ware downside wall one week, the circle plane of second buckle was parallel with the plane of the bottom wall；Institute
The movable ware downside wall stated is equipped at least one the first buckle of circle, and first snap collar is around activity ware downside wall one week, institute
The circle plane for stating the first buckle is parallel with the plane of the bottom wall；First buckle and second buckle can be tight
It is close chimeric, to maintain to be in the technical characteristic above the first ware downside wall without activity ware downside wall described under external force；
It is described when along the activity ware downside wall from activity ware downside wall to the direction of the first ware downside wall, is applied force to
The embedded structure of first buckle and second buckle can unclamp, and movable ware downside wall can be along the first ware downside wall at this time
Direction moves in parallel；The diameter of the ware lid side wall is greater than the first ware downside wall；The diameter of the bottom wall is greater than the lid
Wall；The height of the activity ware downside wall and the sum of the height of the first ware downside wall, less than the height of the ware lid side wall；
The culture dish further includes that at least one surround the second ware downside wall holded up from bottom wall, and the height of the second ware downside wall is less than
The diameter of the ware lid side wall, the second ware downside wall is greater than ware lid side wall, and the first ware downside wall and the second ware downside wall are located at
The same side of the bottom wall；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the mark zone is by wall surfaces
It is formed through frosted processing；It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in convex lens shape,
And it is thickeied by the tegmental wall center and is formed；In the bottom wall, the grid line equipped with vertical and horizontal orthogonal crossover, the side
Bottom wall is divided into the region of several squares or approximating square, the area equation of the square area by ruling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810988992.1A CN108913585A (en) | 2018-08-28 | 2018-08-28 | A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810988992.1A CN108913585A (en) | 2018-08-28 | 2018-08-28 | A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108913585A true CN108913585A (en) | 2018-11-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810988992.1A Pending CN108913585A (en) | 2018-08-28 | 2018-08-28 | A kind of extrusion type leakproof easily marks visual criss-cross method surface sampling culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108913585A (en) |
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2018
- 2018-08-28 CN CN201810988992.1A patent/CN108913585A/en active Pending
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181130 |