CN108893293A - One kind splitting formula out and easily marks visual criss-cross method surface sampling culture dish - Google Patents

Abstract

Formula out, which is split, the invention discloses one kind easily marks visual criss-cross method surface sampling culture dish, the culture dish includes ware bottom and ware lid, it can split set on the transversal crack mouth of the first ware downside wall and longitudinal crack mouth when stressed, it is detached from movable ware downside wall, the height of the first ware downside wall is caused to be lower than the plane of solid medium, therefore the operation of surface microorganism sampling is easier, mark zone can make markers work more efficient, observation window can make the observation of minute colony more convenient, and grid line can simplify the counting process of bacterium colony.

Description

One kind splitting formula out and easily marks visual criss-cross method surface sampling culture dish

Technical field

Formula out, which is split, the present invention relates to a kind of experiment equipment, especially one kind easily marks visual criss-cross method surface sampling culture Ware.

Background technique

Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity Incomplete culture；In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working；These disadvantages It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold Easily cause the waste of human resources.Existing culture dish is mostly glass material, need to could be marked with permanent pen, but oiliness The person's handwriting of marking pen need to could be wiped with special organic solvent, therefore when multiple culture dishes are reused, the not side of label Just it be easy to cause and obscures.When the bacterium colony for the target culture that the culture medium in culture dish is grown is smaller, need to use microscope By bacterium colony amplification by observe bacterium colony it is accurate in the form of, need to open in culture dish during magnify bacterium colony, this possibility Pollution or the leakage of harmful culture can be brought.When the colony counts for the target culture that the culture medium in culture dish is grown are huge When big, the counting process of bacterium colony is then comparatively laborious.

Summary of the invention

For disadvantages mentioned above, the purpose of the present invention is to provide the formulas out of splitting that a kind of structure is simple and convenient to operate easily to mark Visual criss-cross method surface sampling culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, mention The accuracy of high test result improves working efficiency.

Visual criss-cross method surface sampling culture dish, to reach the purpose, what the present invention used are easily marked for splitting formula out Technical solution is：One kind splitting formula out and visual criss-cross method surface sampling culture dish, including ware bottom and relaxation easily marked to be covered in ware Ware lid on bottom, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up, institute from tegmental wall The ware bottom stated includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware downside wall is equipped with At least one circle transversal crack mouth, the transversal crack choma around described first ware downside wall one week, the transversal crack mouth Circle plane is parallel with the plane of the bottom wall；The first ware downside wall is divided into movable ware by the transversal crack mouth Downside wall and fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is laterally to split Line wall, the thickness of the transversal crack wall is less than the first ware downside wall；It is vertical that the movable ware downside wall is equipped at least one Connected in the movable ware downside wall of the longitudinal crack mouth of the transversal crack mouth, longitudinal crack mouth two sides by longitudinal crack wall It connects, the thickness of the longitudinal crack wall is less than the first ware downside wall；The diameter of the ware lid side wall is greater than first ware bottom side Wall；The diameter of the bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the height of the fixed ware downside wall The sum of, less than the height of the ware lid side wall；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the label Area is formed by wall surfaces through frosted processing；At least one transparent observation window, the observation window are additionally provided in the tegmental wall In convex lens shape, and is thickeied by the tegmental wall center and formed；In the bottom wall, the side equipped with vertical and horizontal orthogonal crossover Bottom wall is divided into the region of several squares or approximating square by ruling, the grid line, the square area Area equation.

Compared with the prior art, the beneficial effects of the invention are as follows：The longitudinal crack wall and transversal crack wall of the culture dish Thickness very little, be easy to rupture under external force, when applying force to the movable ware downside wall, the first ware bottom can be made Side wall splits at longitudinal crack mouth and transversal crack mouth, with removal activity ware downside wall, and then makes the height of the first ware downside wall Degree decreases below the plane of solid medium, and the solid culture base plane is directly covered in determinand body surface when use Face can reach the purpose of sampling microorganisms from surface of object.Therefore the structure of the culture dish is simple, easy to use, Ke Yijian The process for changing sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, improve simultaneously Test result accuracy and working efficiency, mark zone can make markers work more efficient, and observation window can make the observation of minute colony more Convenient, grid line can simplify the counting process of bacterium colony.

Detailed description of the invention

Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.

Fig. 1 is the structural schematic diagram for splitting formula out and easily marking visual criss-cross method surface sampling culture dish；Fig. 2 is provided at described The schematic diagram of the grid line of bottom wall；Fig. 3 is the cross-sectional view of the movable ware downside wall；Fig. 4 is first ware bottom side The partial enlargement diagram of wall；Respectively number is respectively in figure：

1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 7 be the first ware downside wall, and 71 be movable ware bottom Side wall, 72 be transversal crack mouth, and 73 be transversal crack wall, and 74 be longitudinal crack mouth, and 75 be longitudinal crack wall, and 76 be fixed ware bottom Side wall, 20 be frosted mark zone, and 21 be observation window, and 23 be grid line.

Specific embodiment

It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.

In the discussion of specific embodiment, the shape split formula out and easily mark visual criss-cross method surface sampling culture dish Circle is preferably selected, still, those skilled in the art should be understood that it is suitable for such as ellipse, square Shape or other shapes suitable for culture dish.

Formula out, which is split, the invention discloses one kind easily marks visual criss-cross method surface sampling culture dish, including ware bottom 2 and pine The ware lid 1 that relaxation is covered on ware bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid side holded up from tegmental wall 3 Wall 5, the ware bottom 2 includes bottom wall 4 and at least one surround the first ware downside wall 7 holded up from bottom wall 4；First ware Downside wall 7 is equipped at least one circle transversal crack mouth 72, and the transversal crack mouth 72 is surround described first ware downside wall 7 one weeks, The circle plane of the transversal crack mouth 72 is parallel with the plane of the bottom wall 4；The first ware downside wall 7 is by the cross Movable ware downside wall 71 and fixed ware downside wall 76 are divided into crackle mouth 72；The movable ware downside wall 71 is consolidated with described The coupling part for determining ware downside wall 76 is transversal crack wall 73, and the thickness of the transversal crack wall 73 is less than the first ware downside wall 7； The movable ware downside wall 71 be equipped at least one perpendicular to the transversal crack mouth 72 longitudinal crack mouth 74, the longitudinal direction The movable ware downside wall 71 of 74 two sides of crackle mouth is connected by longitudinal crack wall 75, and the thickness of the longitudinal crack wall 75 is less than the One ware downside wall 7.Features described above splits the basic knot that formula out easily marks visual criss-cross method surface sampling culture dish described in having determined Structure, wherein the thickness very little of longitudinal crack wall 75 and transversal crack wall 73, is easy to rupture under external force, makes the first ware bottom side Wall 7 splits at longitudinal crack mouth 74 and transversal crack mouth 72, and movable ware downside wall 71 can be from fixed ware downside wall 76 at this time Upper disengaging.And enough fine jades are injected when cultivating inner cavity as culture inner cavity in the space that the first ware downside wall 7 and bottom wall 4 are formed When rouge culture medium（The liquid level of the culture medium is higher than the transversal crack mouth 72）, after the culture medium solidification, apply and make Firmly to the movable ware downside wall 71, the first ware downside wall 7 is made to split at longitudinal crack mouth 74 and transversal crack mouth 72, with Removal activity ware downside wall 71, and then the height of fixed ware downside wall 76 is made to decrease below the plane of the agar medium, The agar medium plane is directly covered in object under test surface when use, that is, can reach sampling microorganisms from surface of object Purpose, avoid and carry out the tedious steps eluted again after microorganism acquisition using cotton swab or filter paper, greatly simplify The process of sampling microorganisms from surface of object, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, simultaneously Improve test result accuracy and working efficiency.

Split in the embodiment that formula easily marks visual criss-cross method surface sampling culture dish out described, the bottom wall 4 it is straight Diameter is greater than the diameter of the tegmental wall 3, and the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, and ware lid side wall 5 can only It is supported in bottom wall 4.In addition, the height of the activity ware downside wall 71 and the sum of the height of the fixed ware downside wall 76, are less than The height of the ware lid side wall 5, this feature can limit when ware lid 1 and the fastening of ware bottom 2 activity ware downside wall 71 and tegmental wall 3 it Between there are certain gaps, prevent the agar medium from contacting with tegmental wall 3.

In the outer surface of the tegmental wall 3, it is equipped at least one mark zone 20, the mark zone 20 is passed through by 3 surface of tegmental wall Frosted processing is formed；The rough surface of the mark zone 20, common pencil, wax crayon, white board marker etc. can be in mark zones 20 Apparent person's handwriting is formed, and the person's handwriting is washed with water and can easily be removed, and keeps the markers work of the culture dish more square Just efficiently.

The tegmental wall 3 is additionally provided at least one transparent observation window 21, and the observation window 21 is in convex lens shape, and by The center of the tegmental wall 3 thickeies and is formed, therefore the observation window 21 can be made micro- with the bacterium colony of amplification target culture The observation of petite is more convenient, and can prevent the possible dirt because opening ware lid 1 and ware bottom 2 during observation bacterium colony The leakage of dye or harmful culture.

In the bottom wall 4, the grid line 23 equipped with vertical and horizontal orthogonal crossover, bottom wall 4 is divided by the grid line 23 The region of several squares or approximating square, the area equation of the square area；When the culture medium institute in culture dish When the colony counts of the target culture of growth are huge, the bacterium colony count results in a square area can be used, multiplied by phase The multiple answered（In the embodiment shown in Figure 2, the side length of the square area is 1 centimetre, agar medium in ware bottom 2 Diameter is 10 centimetres, then its area is 78.5 square centimeters, so multiple is 78.5）To indicate all clump counts of culture dish, Therefore the counting process of bacterium colony is simplified.

Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair Bright protection scope.

Claims (1)

1. one kind, which splits formula out, easily marks visual criss-cross method surface sampling culture dish, it is covered on ware bottom including ware bottom and relaxation Ware lid, it is characterised in that：The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up, the ware from tegmental wall Bottom includes bottom wall and at least one surround the first ware downside wall holded up from bottom wall；The first ware downside wall is equipped at least one Transversal crack mouth is enclosed, for the transversal crack choma around described first ware downside wall one week, the circle of the transversal crack mouth was flat Face is parallel with the plane of the bottom wall；The first ware downside wall is divided into movable ware downside wall by the transversal crack mouth With fixed ware downside wall；The coupling part of the movable ware downside wall and the fixation ware downside wall is transversal crack wall, The thickness of the transversal crack wall is less than the first ware downside wall；The movable ware downside wall is equipped at least one perpendicular to described The movable ware downside wall of the longitudinal crack mouth of transversal crack mouth, longitudinal crack mouth two sides is connected by longitudinal crack wall, institute The thickness of longitudinal crack wall is stated less than the first ware downside wall；The diameter of the ware lid side wall is greater than the first ware downside wall；Institute The diameter for stating bottom wall is greater than the tegmental wall；The height of the activity ware downside wall and the sum of the height of the fixed ware downside wall, Less than the height of the ware lid side wall；In the outer surface of the tegmental wall, it is equipped at least one mark zone, the mark zone is by covering Wall surface is formed through frosted processing；It is additionally provided at least one transparent observation window in the tegmental wall, the observation window is in convex lens Mirror shape, and thickeied by the tegmental wall center and formed；In the bottom wall, the grid line equipped with vertical and horizontal orthogonal crossover, Bottom wall is divided into the region of several squares or approximating square, the area phase of the square area by the grid line Deng.