CN206208629U - A kind of immunofluorescent staining slide - Google Patents
A kind of immunofluorescent staining slide Download PDFInfo
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- CN206208629U CN206208629U CN201621269985.9U CN201621269985U CN206208629U CN 206208629 U CN206208629 U CN 206208629U CN 201621269985 U CN201621269985 U CN 201621269985U CN 206208629 U CN206208629 U CN 206208629U
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- slide
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- cell
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Abstract
The utility model provides a kind of immunofluorescent staining slide, including slide body, the head of the slide body is provided with Handheld Division, and the upper surface of the slide body is provided with hydrophobic frame, the hydrophobic frame is hydrophobic coating layer, and the thickness of dope layer is 5 μm~20 μm.The utility model sets hydrophobic frame by slide surface, the flowing of liquid is limited to hydrophobic inframe, and cell can be fully contacted with staining reagent in hydrophobic inframe, be conducive to the dyeing of cell.Additionally, slide of the present utility model sets the hydrophobic frame that border width determines by slide body, cell sample zone boundary can be quickly determined in micro- sem observation, be more suitable for the use of the automation whole sample area of observation.Slide of the present utility model can carry out oneself making to existing conventional slide, and difficulty of processing is low and low cost, practical.
Description
Technical field
The utility model is related to laboratory consumptive material field, specifically a kind of immunofluorescent staining slide.
Background technology
Slide is for placing the sheet glass of experiment material in experiment.It is right in the Coloration experiment of cell and tissue
Most of cytoscopy needs to use slide.
Cellular immunofluorescence is that set up on the basis of immunology, biochemistry and microscopy is emerging
Technology.The technology needs to carry out fluorescent staining to target cell, so as to qualitatively or quantitatively determine to cell under the microscope.
According to colouring method, solid-state decoration method and liquid dying process can be divided into.Wherein, solid-state decoration method be by cell on slide
Carry out creep plate or smear is realized.But existing slide is used for the effect on driving birds is not good that cell is fixed, cell is easy when solid-state is dyeed
Come off from slide, influence the accuracy of measurement result.For example, to during dropwise addition cell suspension, cell hangs on existing slide
Liquid product is general no more than 50 μ L, and otherwise cell suspension can be excessive on slide, causes the loss of cell sample, especially
During to cover glass is covered on slide, the loss of cell is more serious.Meanwhile, existing slide is not applied specifically
Panel region, it is impossible to carry out microscope automatic scanning, it is impossible to carry out differential counting to whole cells, can only draw field of microscope
Under subregion cellular informatics.
Anticreep slide is used for cell to be fixed and cell dyeing.Anticreep slide is to carry out functionalization in slide surface
Treatment, to strengthen the adhesive force of cell, is easy to cell dyeing.Such as poly-D-lysine slide, silication anticreep slide or
Positive charge anticreep slide etc..But anticreep slide improves the cost of slide, and quality is difficult to control to, easily influence
Microscope observing effect.
The content of the invention
The deficiency that main purpose of the present utility model is directed to prior art presence provides a kind of immunofluorescent staining
With slide, simply, the effect that cell is fixed is good, is easy to microscope automatic scanning for the slide configurations.
The utility model is achieved by the following technical solution：A kind of immunofluorescent staining slide, it is special
Levy and be：The slide includes slide body, and the head of the slide body is provided with Handheld Division, the slide body
Upper surface be provided with hydrophobic frame, the hydrophobic frame is hydrophobic coating layer, and the thickness of dope layer is 5 μm~20 μm.
Preferably, the hydrophobic frame is square hydrophobic frame, and the border width of the square hydrophobic frame is 1mm~2mm.
Preferably, the Handheld Division includes the frosted layer positioned at slide body head upper surface.
Preferably, the Handheld Division both sides are provided with deep-slotted chip breaker.
Preferably, the upper and lower arc seamed edge of the deep-slotted chip breaker is chamfering or fillet seamed edge.
Preferably, the corner of the slide body is fillet.
The utility model has the advantages that：
The utility model sets hydrophobic frame by slide surface, the flowing of liquid is limited to hydrophobic inframe, cell
Can be fully contacted in hydrophobic inframe with staining reagent, be conducive to the dyeing of cell.Also, the utility model ensure that hydrophobic frame
Thickness, can accommodate more liquid, and mounting is not likely to produce aeration observation result, will not also damage cell by pressure.Meanwhile,
The utility model is provided with the frosted layer Handheld Division that can be marked with writing words in slide head, and is set in Handheld Division both sides
Putting can be in order to the deep-slotted chip breaker of crawl, it is ensured that on the premise of the size of slide meets automatic read tablet requirement, can facilitate to carrying
Slide is operated.Additionally, slide of the present utility model on slide body by setting the hydrophobic of border width determination
Frame, cell sample zone boundary can be quickly determined in micro- sem observation, be more suitable for the automation whole sample area of observation
Use.Slide of the present utility model can carry out oneself making to existing conventional slide, and difficulty of processing is low and cost
It is low, practical.
Brief description of the drawings
Fig. 1 is slide front view of the present utility model；
Fig. 2 is slide left view of the present utility model；
Wherein：1- slides body, 2- Handheld Divisions, the hydrophobic frames of 3-, 4- frames, 5- frosted layers, 6- deep-slotted chip breakers, 7- chamfering ribs
Side, 8- fillets.
Specific embodiment
The utility model is further illustrated below in conjunction with accompanying drawing：
As shown in figure 1, immunofluorescent staining of the present utility model includes slide body 1, slide with slide
The head of body 1 is provided with Handheld Division 2, and the upper surface of slide body 1 is provided with hydrophobic frame 3, and hydrophobic frame 3 is hydrophobic coating layer, is applied
The thickness of the bed of material is 10 μm.Dope layer is blocked up to cause covered easily to produce bubble, excessively thin to cause covered
Extruding cell.Hydrophobic frame 3 is square hydrophobic frame, and the width of frame 4 is 1.5mm, and the inside casing size of hydrophobic frame 3 is 16 × 16mm, the chi
Under very little hydrophobic frame 3 and thickness of coating layer, the amount of liquid of up to 500 μ L can be accommodated, be the 10 of existing slide liquid carrying capacity
Times.
Handheld Division 2 includes the frosted layer 5 positioned at the head upper surface of slide body 1.Experimenter can be on frosted layer 5
Written indicia is distinguishing slide.The both sides of Handheld Division 2 are provided with deep-slotted chip breaker 6.It is easy to the hand-held slide of experimenter or gripping to carry
Slide.The upper and lower arc seamed edge of the deep-slotted chip breaker 6 is chamfer edge 7.The corner of slide body 1 is fillet 8.Reality can be prevented
Test personnel and scratch hand in hand-held slide.The utility model is ensureing the slide original standard-sized base of slide of maintenance
On plinth, it is ensured that the safety of experimenter.
In practical application, after elder generation in the hydrophobic frame 3 of slide of the present utility model 1 to the μ L of cell suspension about 500 are added dropwise,
Addition dyeing liquor, covered, sealing up piece glue in cover glass surrounding carries out mounting, and automatic read tablet is seen under the microscope
Examine.Observation result shows that cell can be evenly distributed in the frame 4 of hydrophobic frame 3, be conducive to microexamination, is adapted to microscope
Full-automatic extensive examination cell.
For those skilled in the art, the premise of the utility model embodiment principle is not being departed from
Under, some improvements and modifications can also be made, these improvements and modifications are also considered as the protection domain of the present embodiment.
Claims (6)
1. a kind of immunofluorescent staining slide, it is characterised in that：The slide includes slide body (1), institute
The head for stating slide body (1) is provided with Handheld Division (2), and the upper surface of the slide body (1) is provided with hydrophobic frame (3), institute
It is hydrophobic coating layer to state hydrophobic frame (3), and the thickness of dope layer is 5 μm~20 μm.
2. immunofluorescent staining slide according to claim 1, it is characterised in that：The hydrophobic frame (3) is
Square hydrophobic frame, frame (4) width of the square hydrophobic frame is 1mm~2mm.
3. immunofluorescent staining slide according to claim 1, it is characterised in that：Handheld Division (2) bag
Include the frosted layer (5) positioned at slide body (1) head upper surface.
4. immunofluorescent staining slide according to claim 1, it is characterised in that：The Handheld Division (2) two
Side is provided with deep-slotted chip breaker (6).
5. immunofluorescent staining slide according to claim 4, it is characterised in that：On the deep-slotted chip breaker (6),
Arc lower seamed edge is chamfering or fillet seamed edge.
6. immunofluorescent staining slide according to claim 1, it is characterised in that：The slide body
(1) corner is fillet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621269985.9U CN206208629U (en) | 2016-11-25 | 2016-11-25 | A kind of immunofluorescent staining slide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621269985.9U CN206208629U (en) | 2016-11-25 | 2016-11-25 | A kind of immunofluorescent staining slide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206208629U true CN206208629U (en) | 2017-05-31 |
Family
ID=58746913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621269985.9U Active CN206208629U (en) | 2016-11-25 | 2016-11-25 | A kind of immunofluorescent staining slide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206208629U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107632384A (en) * | 2017-10-26 | 2018-01-26 | 桂林优利特医疗电子有限公司 | Flange slide and anti-dry cover glass and wire frame preparation method |
| CN107991159A (en) * | 2017-11-20 | 2018-05-04 | 珠海伊斯佳科技股份有限公司 | A kind of method by skin keratinocytes morphological assessment skin |
| CN109358421A (en) * | 2018-11-21 | 2019-02-19 | 佛山科学技术学院 | It is a kind of with the Novel glass slide for checking stereoscopic effect |
-
2016
- 2016-11-25 CN CN201621269985.9U patent/CN206208629U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107632384A (en) * | 2017-10-26 | 2018-01-26 | 桂林优利特医疗电子有限公司 | Flange slide and anti-dry cover glass and wire frame preparation method |
| CN107991159A (en) * | 2017-11-20 | 2018-05-04 | 珠海伊斯佳科技股份有限公司 | A kind of method by skin keratinocytes morphological assessment skin |
| CN109358421A (en) * | 2018-11-21 | 2019-02-19 | 佛山科学技术学院 | It is a kind of with the Novel glass slide for checking stereoscopic effect |
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