CN209858582U - Mould for manufacturing flat plate type biological in-vitro diagnostic reagent detection sheet - Google Patents
Mould for manufacturing flat plate type biological in-vitro diagnostic reagent detection sheet Download PDFInfo
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- CN209858582U CN209858582U CN201920239095.0U CN201920239095U CN209858582U CN 209858582 U CN209858582 U CN 209858582U CN 201920239095 U CN201920239095 U CN 201920239095U CN 209858582 U CN209858582 U CN 209858582U
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Abstract
The utility model provides a mould for manufacturing a flat plate type in vitro diagnostic reagent detection sheet, which comprises a mould plate body; the improvement is that the mould plate body is an integrally formed rectangular stainless steel plate body, six integrally formed caulking grooves are arranged on the plate body on the front surface of the rectangular stainless steel plate body and are correspondingly embedded with the partition plate of the frame, the mould plate body is evenly divided into five areas which have the same area and shape and are relatively independent through the six caulking grooves, and each area corresponds to a reaction area on the flat plate body respectively; the frame is composed of a plastic plate body and a plurality of partition plates which are integrally formed on the plastic plate body; the plane plate body is fixed on the plate surface of the frame, which is far away from the partition plate, and a finished product of the die is formed; when the mold is used for manufacturing the flat plate type in-vitro biological diagnosis reagent detection sheet, the glue is easy to glue, does not shift or deform, is convenient and fast to operate, and improves the product percent of pass.
Description
Technical Field
The utility model relates to a mould for manufacturing a flat plate type in vitro diagnostic reagent detection sheet.
Background
At present, the immunological detection technology and products thereof are widely applied to a plurality of detection fields of major infectious diseases, autoimmune diseases, tumor markers, food safety, prenatal and postnatal care and the like, in an indirect immunofluorescence assay detection test, the detection of the coated antigen matrix is necessary, and a flat plate type detection piece device used by the detection technology is an important tool, so that the manufacture of the flat plate type detection piece device is particularly important. At present, a certain antigen or a plurality of antigens are coated on the fixed position of a glass slide, and 1 to 10 fixed positions are formed on each glass slide, so that the synchronous detection of 1 to 10 patients can be simultaneously carried out in the detection link, and if the manufacture of an in vitro diagnostic reagent detection device is not over, the mutual pollution of the samples of the patients is possibly caused. Accordingly, there is a need for improved fabrication of flat panel test strip devices.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a main aim at overcomes the above-mentioned shortcoming that current product exists, and provides a mould of preparation flat external diagnostic reagent test piece of living beings for have easy rubber coating, do not shift, indeformable and the efficiency of convenient preparation when preparation flat external diagnostic reagent test piece of living beings, effectively improve work efficiency and product percent of pass, reduce cost.
The purpose of the utility model is realized by the following technical scheme.
The utility model discloses a mould for manufacturing a flat plate type in vitro diagnostic reagent detection sheet, which comprises a mould plate body; the mold plate body is an integrally formed rectangular stainless steel plate body, six integrally formed caulking grooves are formed in the plate body on the front side of the rectangular stainless steel plate body and are correspondingly embedded with a partition plate of a frame, the mold plate body is evenly divided into five areas which are identical in area and shape and relatively independent through the six caulking grooves, and each area corresponds to a reaction area on the flat plate body; the frame is composed of a plastic plate body and a plurality of partition plates which are integrally formed on the plastic plate body; the flat plate body is adhered and fixed on the plate surface of the frame deviating from the partition plate through an adhesive to form a finished product of the die.
The six caulking groove arrangement positions integrally formed on the front surface of the plate body of the mold comprise four caulking grooves which are respectively positioned on two short edges of the plate body and are arranged in parallel and equidistantly along a long edge, the six caulking grooves equally divide the front surface of the plate body of the mold into five rectangular areas with equal areas, the length of the areas is equal to the length of a glass slide, and the width of the areas is equal to the width of the glass slide; and each caulking groove is provided with an inner concave body with a semicircular cross section at a position 2 +/-0.5 mm away from one corner end, the diameter of the inner concave body is 1.5 +/-0.5 mm, and the depth of the inner concave body is 2 +/-0.2 mm; the frame is a rectangular hollow frame body made of plastic materials, the length of the rectangular hollow frame body is the same as that of the mould plate body, the width of the rectangular hollow frame body is the same as that of the mould plate body, six partition plates made of plastic materials and perpendicular to the frame body are integrally formed at equal intervals along the long edge of the rectangular hollow frame body, the arrangement positions of the six partition plates correspond to the positions of six caulking grooves integrally formed on the plate body on the front surface of the mould plate body, the length of the middle partition plate is equal to the length of the six caulking grooves, and the width of the middle partition; a cylinder corresponding to the inner concave body is respectively arranged at the corresponding positions of the partition board and the inner concave body of the caulking groove, the diameter of the cylinder corresponds to that of the inner concave body, the height of the cylinder corresponds to that of the inner concave body, and the cylinder is inserted into the inner concave body to fix the detection substrate; the flat plate body is made of common glass, the length of the flat plate body is equal to that of the mould plate body, and the width of the flat plate body is equal to that of the mould plate body; the positions corresponding to five rectangular areas with equal areas, which are evenly divided by six caulking grooves, on the front surfaces of the plane plate body and the die plate body are reaction areas, ten hollowed-out sample adding areas are arranged on each reaction area in a double-row equidistant mode, and the length of each sample adding area is 5 +/-0.5 mm, and the width of each sample adding area is 5 +/-0.5 mm.
The mold for manufacturing the flat plate type in-vitro diagnostic reagent detection sheet is characterized in that the frame body and the partition plates on the integrally formed frame body are made of polymethyl methacrylate (PMMA); and the sample adding area on the plane plate body is integrally formed by silk screen printing.
The beneficial effects of the utility model, through the flat biological in vitro diagnostic reagent detection piece of this mould preparation, easy and simple to handle, swift can improve work efficiency greatly. The detection piece is placed on the die plate body through the frame and the partition plate, is straight and does not deform, the size of the reaction area is consistent, and the position of the sample adding area is fixed, so that the glass slide can be ensured to be in embedded contact with the reaction area on the plane plate body every time, a sealing structure is formed, the volatilization of a sample is effectively avoided, and the detection accuracy is improved.
Drawings
Fig. 1 is a top view of the overall structure of the present invention.
Fig. 2 is the schematic diagram of the structure of the die plate of the present invention.
Fig. 3 is a schematic view of the frame structure of the present invention.
Fig. 4 is an exploded view of the overall structure of the present invention.
Fig. 5 is a diagram illustrating an implementation state of the present invention.
The main reference numbers in the figures illustrate: 1 mould plate body, 2 concave bodies, 4 caulking grooves, 5 plane plate bodies, 6 frames, 7 partition plates, 8 columns, 9 flat plate type in vitro diagnosis reagent detection sheets, 10 sample adding areas and 11 reaction areas.
Detailed Description
As shown in fig. 1, fig. 2, fig. 3, fig. 4 and fig. 5, the mold for manufacturing a flat plate type in vitro diagnostic reagent test strip of the present invention comprises a mold plate body; the improvement is that the die plate body 1 is an integrally formed rectangular stainless steel plate body, six integrally formed caulking grooves 4 are arranged on the plate body on the front surface of the rectangular stainless steel plate body and are correspondingly embedded with the partition plates 7 of the frame 6, the die plate body 1 is evenly divided into five areas which have the same area and shape and are relatively independent through the six caulking grooves, and each area corresponds to the reaction area 11 on the flat plate body 5; the frame 6 is composed of a plastic plate body and a plurality of partition plates 7 which are integrally formed on the plastic plate body; the flat plate body 5 is fixed on the surface of the frame 6, which is far away from the partition board, by bonding through an adhesive, and a finished product of the die is formed.
As shown in fig. 1, fig. 2, fig. 3, fig. 4, and fig. 5, the present invention provides a mold for manufacturing a flat plate type in vitro diagnostic reagent detection sheet, wherein the six caulking grooves 4 integrally formed on the front surface of the mold plate 1 include two caulking grooves 4 respectively located on two short sides of the plate and four caulking grooves 4 arranged in parallel and equidistantly along a long side, the six caulking grooves 4 equally divide the front surface of the mold plate 1 into five rectangular regions with equal areas, the length of the regions is equal to the length of a glass slide, and the width of the regions is equal to the width of the glass slide; and each caulking groove is provided with an inner concave body 2 with a semicircular cross section at a position 2 +/-0.5 mm away from one corner end, the diameter of the inner concave body 2 is 1.5 +/-0.5 mm, and the depth of the inner concave body is 2 +/-0.2 mm;
the frame 6 is a rectangular hollow frame body made of plastic materials, the length of the rectangular hollow frame body is the same as that of the mould plate body 1, the width of the rectangular hollow frame body is the same as that of the mould plate body 1, six partition plates 7 made of plastic materials and perpendicular to the frame body are integrally formed at equal intervals along the long edge of the rectangular hollow frame body, the arrangement positions of the six partition plates 7 correspond to the positions of six caulking grooves integrally formed on the plate body on the front surface of the mould plate body 1, the length of each middle partition plate 7 is equal to that of the six caulking grooves, and the width of each middle partition plate; a cylinder 8 corresponding to the inner concave body 2 is respectively arranged at the positions of the clapboard 7 corresponding to the inner concave body 2 of the caulking groove 4, the diameter of the cylinder 8 corresponds to the diameter of the inner concave body, the height of the cylinder corresponds to the depth of the inner concave body, and the cylinder is inserted into the inner concave body and used for fixing a detection substrate; the flat plate body 5 is made of common glass, the length of the flat plate body is equal to that of the mould plate body 1, and the width of the flat plate body is equal to that of the mould plate body 1; the position corresponding to five rectangular areas with equal area equally divided by six caulking grooves 4 on the front surface of the plane plate body and the front surface of the mould plate body 1 is a reaction area 11, ten hollowed-out sample adding areas 10 are arranged on each reaction area 11 in double rows at equal intervals, and the length and the width of each sample adding area are respectively 5 +/-0.5 mm and 5 +/-0.5 mm. The frame 6 and the partition plates on the integrally formed frame are made of polymethyl methacrylate (PMMA); the sample adding area 10 on the plane plate body 5 is integrally formed by silk screen printing.
Example 1, as shown in fig. 5:
adopt the utility model discloses the mould preparation flat biological in vitro diagnostic reagent of preparation flat biological in vitro diagnostic reagent detects piece:
1. preparing: the slide is taken out, and the package is cut open after the slide is recovered to the room temperature. Dissolving the washed PBS powder into 1L by using distilled water, adding 2ml of Tween, and uniformly mixing for washing and specimen dilution.
2. Diluting: the serum to be tested was diluted 1:10 with the washing solution.
3. Sample adding: diluted blood and positive and negative controls, 25 mul/well, are respectively added into the corresponding sample adding areas 10 of the utility model. Note that: avoiding air bubbles from being generated during sample adding.
4. And (3) incubation: the surface of the glass slide antigen is reversely buckled on the corresponding sample adding area 10 of the utility model, and the incubation is carried out for 30min at room temperature. Note that: ensuring that each sample is able to contact the antigen matrix without contact between the samples.
5. Washing: taking out the glass slide, washing the reaction solution from one side with a washing solution, soaking the glass slide in a washing tank, standing for 5min, and washing with distilled water for 1 time.
6. Sample adding: a fluorescent secondary antibody was added to the sample addition part 10 of the detection plate 5 at a concentration of 25. mu.l/well. Note that: avoiding air bubbles from being generated during sample adding.
7. And (3) incubation: after the excess liquid on the periphery and the reverse side of the antigen sheet is wiped off by paper, the antigen side of the glass slide is covered on the sample adding area 10 corresponding to the detection plane plate body 5, and the glass slide is incubated for 30min at room temperature. Note that: ensuring that each sample is able to contact the antigen matrix without contact between the samples.
8. Washing: and (5) repeating the step.
9. Sealing: the encapsulated tablets were dropped onto a cover slip. After excess liquid on the periphery and the reverse side of the antigen slide is wiped off by paper, the antigen matrix of the slide is placed downwards on a prepared cover glass, and the operation of sealing the slide is finished.
10. And (4) observing results: and (5) observing the result under a fluorescence microscope.
The flat plate type in vitro diagnostic reagent detection piece manufactured by the mould for manufacturing the flat plate type in vitro diagnostic reagent detection piece provided by the utility model can ensure that the position of the glass slide is fixed because the size of the reaction zone is consistent with that of the glass slide, and can form embedded contact with the flat plate type in vitro diagnostic reagent detection device every time, thereby forming a closed structure and effectively avoiding the volatilization of a sample; the antigen matrix area coated on the glass slide corresponds to the position of the sample adding area in the reaction area of the plane plate body, so that the effective reaction of the antigen and the sample can be ensured, and the detection accuracy is improved; each flat plate type in vitro diagnosis reagent detection sheet comprises five reaction areas and 10 sample adding areas, 50 samples can be detected at most at one time, and each reaction area is independently arranged, so that the detection efficiency and the product detection accuracy can be greatly improved, and the manual operation cost is effectively reduced.
The content that will not be described in this embodiment is the prior art, and therefore, will not be described again.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any form, and all the technical solutions of the present invention are applicable to any simple modification, equivalent change and modification of the above embodiments.
Claims (3)
1. A mould for manufacturing a flat plate type in-vitro diagnosis reagent detection sheet comprises a mould plate body; the mold plate body (1) is an integrally formed rectangular stainless steel plate body, six integrally formed caulking grooves (4) are formed in the plate body on the front face of the rectangular stainless steel plate body and are correspondingly embedded with partition plates (7) of a frame (6), the mold plate body (1) is evenly divided into five relatively independent areas with the same area and shape through the six caulking grooves, and each area corresponds to a reaction area (11) on the flat plate body (5); the frame (6) is composed of a plastic plate body and a plurality of partition plates (7) which are integrally formed on the plastic plate body; the plane plate body (5) is adhered and fixed on the surface of the frame (6) departing from the clapboard through an adhesive to form a finished product of the die.
2. The mold for manufacturing the flat plate type in vitro diagnostic reagent test piece according to claim 1, wherein the six caulking grooves (4) integrally formed on the front surface of the mold plate body (1) are arranged at positions comprising four caulking grooves (4) which are respectively positioned on two short sides of the plate body and are arranged at equal intervals along the long side, the six caulking grooves (4) equally divide the front surface of the mold plate body (1) into five rectangular areas with equal area, the length of the rectangular area is equal to the length of a glass slide, and the width of the rectangular area is equal to the width of the glass slide; and each caulking groove is provided with an inner concave body (2) with a semicircular cross section at a position 2 +/-0.5 mm away from one corner end, the diameter of the inner concave body is 1.5 +/-0.5 mm, and the depth of the inner concave body is 2 +/-0.2 mm;
the frame (6) is a rectangular hollow frame body made of plastic; the length of the rectangular hollow frame body is the same as that of the mould plate body (1), and the width of the rectangular hollow frame body is the same as that of the mould plate body (1); six partition plates (7) which are made of plastic materials and are vertical to the rectangular hollow frame body are integrally formed at equal intervals along the long edge, the arrangement positions of the six partition plates (7) correspond to the positions of six caulking grooves which are integrally formed on the front plate body of the die plate body (1), the length of each middle partition plate (7) is equal to the length of the six caulking grooves, and the width of each middle partition plate is equal to the width of the six caulking grooves; a column (8) corresponding to the inner concave body (2) is respectively arranged at the positions of the partition plate (7) corresponding to the inner concave body (2) of the caulking groove (4), the diameter of the column (8) corresponds to the diameter of the inner concave body, and the height of the column corresponds to the depth of the inner concave body, so that the column is inserted into the inner concave body to fix the detection substrate;
the flat plate body (5) is made of ordinary glass, the length of the flat plate body is equal to that of the mould plate body (1), and the width of the flat plate body is equal to that of the mould plate body (1); the front surfaces of the plane plate body and the die plate body (1) are equally divided into five rectangular areas with equal areas through six caulking grooves (4), the corresponding positions are reaction areas (11), ten hollowed-out sample adding areas (10) are arranged on each reaction area (11) in a double-row equidistant mode, and the length of each sample adding area is 5 +/-0.5 mm, and the width of each sample adding area is 5 +/-0.5 mm.
3. The mold for manufacturing the flat plate type in vitro diagnostic reagent test piece according to claim 2, wherein the frame body of the frame (6) and the partition plates on the integrally formed frame body are made of polymethyl methacrylate (PMMA); and the sample adding area (10) on the plane plate body (5) is integrally formed by silk screen printing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920239095.0U CN209858582U (en) | 2019-02-26 | 2019-02-26 | Mould for manufacturing flat plate type biological in-vitro diagnostic reagent detection sheet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920239095.0U CN209858582U (en) | 2019-02-26 | 2019-02-26 | Mould for manufacturing flat plate type biological in-vitro diagnostic reagent detection sheet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209858582U true CN209858582U (en) | 2019-12-27 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920239095.0U Active CN209858582U (en) | 2019-02-26 | 2019-02-26 | Mould for manufacturing flat plate type biological in-vitro diagnostic reagent detection sheet |
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| Country | Link |
|---|---|
| CN (1) | CN209858582U (en) |
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- 2019-02-26 CN CN201920239095.0U patent/CN209858582U/en active Active
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