CN201485471U

CN201485471U - Parallel-type capillary biological chip

Info

Publication number: CN201485471U
Application number: CN2009200344884U
Authority: CN
Inventors: 郭晏海; 颜真
Original assignee: Fourth Military Medical University FMMU
Current assignee: Fourth Military Medical University FMMU
Priority date: 2009-09-10
Filing date: 2009-09-10
Publication date: 2010-05-26
Anticipated expiration: 2019-09-10

Abstract

The utility model relates to a parallel-type capillary biological chip. An original capillary biological chip fixes detection molecules on the inner wall surface or the capillaceous core surface of capillary in a dotting manner or other manners, and the production and the hybrid operation has different difficulties and inconvenience. The capillary biological chip comprises a chip substrate and a cover plate, wherein a plurality of fine concave grooves arranged in parallel and large concave grooves connected with the fine concave grooves are formed on the chip substrate, and the fine concave grooves are marked with different detection molecules. After the chip substrate and the cover plate are integrated, a hybrid zone formed by a plurality of capillaries and a cavity namely a pressure cushion zone connected with the hybrid zone are formed in the chip. When in hybrid detection, samples are sucked in the capillary of the hybrid zone from an opening end of the chip, and rhythmical press on the cushion zone can enable sample solution to shuttle back and forth in a capillary chamber, and to achieve the quick hybridization. Such design not only reduces the production difficulty of the chip, but also enables the biological chip to have the convenient and quick hybrid operation.

Description

Block form kapillary biochip

Affiliated technical field

The utility model relates to a kind of block form kapillary biochip.

Background technology

In the prior art because the hybridization speed in the capillary lumen is fast, amount of samples seldom, be used for biochip technology more and more.The kapillary biochip comprises protein chip and gene chip, and the kapillary protein chip is that specific antibody, antigen molecule are fixed in the kapillary; The kapillary gene chip then is that special dna probe is fixed in the kapillary.In a word, the kapillary biochip is that a large amount of detection molecules are fixed in the kapillary.The structure of capillary chip is broadly divided into three kinds: the one, and directly use kapillary as carrier, at kapillary internal surface fixed test molecule.But because official jargon capillaceous is very little, want to fix multiple different detection molecules respectively at the different sections of kapillary internal surface, the production difficulty is bigger.Company is also arranged with the whole fixing a kind of detection molecules of the internal surface of a capillary, the kapillary that will be fixed with different detection molecules then gathers together, and makes capillary matrix formula chip after the cutting; The 2nd, become capillary core chip (applicant obtains patent) in the capillary cavity with indicating the capillary silk of different detection molecules, being fixed in; The 3rd, utilize micro-processing technology, on sheet glass, silicon chip or organic materials, carve capillary channel earlier, bottom and inboard fixed test molecule in capillary channel cover capillary channel with transparent material then, form capillary lumen, make capillary chip.Above-mentioned capillary chip, or it is big to produce the preparation difficulty, or operation inconvenience when detecting.

Summary of the invention

The purpose of this utility model provides a kind of block form kapillary biochip with respect to the easier production of above-mentioned capillary chip, easier detecting operation.

For achieving the above object, the technical solution of the utility model is:

A kind of kapillary biochip comprises chip substrate and chip cover plate, and its special character is: described chip substrate 2 adopts hybridization region 3 that is provided with many capillary Baltimore grooves that are arranged in parallel and pressure buffer chamber 4 compositions of forming with big Baltimore groove.Detection molecules 5 is individually fixed on the hybridization region 3 of capillary Baltimore groove composition.Chip cover plate 1 is incorporated on the chip substrate 2.

Said chip substrate 2 is combined as a whole by tackiness agent and chip cover plate 1.

Said chip substrate 2 adopts rigid material.

Said chip cover plate 1 adopts organic optical materials.

Description of drawings

Below in conjunction with specific embodiment the utility model is described in further detail.

Fig. 1 is the structural representation of block form kapillary biochip;

Fig. 2 be Fig. 1 overlook drawing figure;

1. chip cover plates among the figure, 2. chip substrate, the 3. hybridization region that constitutes of capillary Baltimore groove, 4. pressure buffer district, 5. capillary Baltimore groove internal fixing has the zone of detection molecules.

Embodiment

Referring to Fig. 1, Fig. 2, the utility model is made of chip substrate 2 and chip cover plate 1.Chip substrate 2 has the hybridization region 3 of many parallel capillary Baltimore grooves by one and pressure buffer district 4 that big Baltimore groove forms constitutes.Chip substrate 2 can adopt slide, silicon chip, plastic sheet, or even rigid material such as tinsel, capillary Baltimore groove hybridization region surface is fixed with different detection molecules 5 respectively by getting ready or spraying the line mode, and detection molecules can adopt antigen, antibody or dna probe etc. as required.After detection molecules fixedly finishes, chip cover plate 1 and chip substrate 2 are bonded into one, just form hybridization region of forming by many parallel kapillaries 3 and cavity---the pressure buffer district 4 that is attached thereto at complete chip internal like this.In the hybridization testing process, sample sucks the kapillary of hybridization region from the opening end of chip, by pressure buffer district 4 rhythm and pace of moving things is arranged is exerted pressure, sample solution is shuttled back and forth in capillary cavity, realizes quick hybridization.

The utility model is with respect to prior art, and its advantage is as follows:

1, be convenient to manufacture: this utility model aborning, the fixed mode of its detection molecules is identical with the conventional planar chip, can use to get ready or spray the line mode and carry out molecule and fix, and has therefore overcome the directly difficulty of fixed test molecule in capillary.

2, crossover operation is convenient: the pressure buffer chamber of the utility model design, and by the rhythm and pace of moving things is arranged exerting pressure in the pressure buffer chamber, the liquid that its inner air can promote in the capillary is discharged; And when removing pressure, sample solution is inhaled into again capillary, so repeatedly can reach the purpose of the hybridization of shuttling back and forth. The speed of comparing hybridization with plane hybridization is faster.

Claims

1. block form kapillary biochip, comprise chip substrate, chip cover plate, it is characterized in that: the chip substrate of described biochip adopts and is provided with that the hybridization region (3) of the capillary Baltimore groove that is arranged in parallel and the vat that is attached thereto---the substrate of pressure buffer district (4), detection molecules is fixed in the capillary Baltimore groove.

2. block form kapillary biochip according to claim 1 is characterized in that: described chip substrate (2) and chip cover plate (1) are combined in simultaneously by tackiness agent.

3. according to the block form kapillary biochip of claim 1 or 2, it is characterized in that: described chip substrate (2) adopts rigid material.

4. according to the block form kapillary biochip of claim 1 or 2, it is characterized in that: described chip cover plate (1) adopts organic optical materials.