CN211179850U

CN211179850U - Magnetic particle light-emitting double-layer micro-fluidic chip and detection system

Info

Publication number: CN211179850U
Application number: CN201921703049.8U
Authority: CN
Inventors: 王东; 范玉霞; 李泉
Original assignee: Shenzhen Huamaixingwei Medical Technology Co ltd
Current assignee: Shenzhen Huamaixingwei Medical Technology Co ltd
Priority date: 2019-10-11
Filing date: 2019-10-11
Publication date: 2020-08-04
Anticipated expiration: 2029-10-11

Abstract

The utility model belongs to the technical field of the luminous immunodetection of micro-fluidic chip, especially, relate to a luminous double-deck micro-fluidic chip of magnetic particle and detecting system. The chip comprises a top plate and a bottom plate, wherein the top plate comprises a sample adding part, a labeled ligand storage part and a sample mixing region, and the sample mixing region is communicated with the sample adding part and the labeled ligand storage part respectively; the bottom plate comprises a flow guide area, a magnetic particle coating part, a cleaning area, a detection area and a cleaning liquid storage part, wherein a groove with the height lower than the bottom wall of the magnetic particle coating part and a flow guide part which is arranged on the groove and connected with the magnetic particle coating part are arranged in the flow guide area. The sample enters from the sample adding part, is mixed with the labeled ligand in the sample mixing area and then enters the groove of the flow guide area, the sample in the groove can be absorbed by capillary action, and due to the action of the cut-off groove and the blocking part, the sample can only enter the magnetic particle coating part from the flow guide part, then is mixed and reacts with the magnetic particle ligand, and after the cleaning area is cleaned, the luminescence detection is realized in the detection area.

Description

Magnetic particle light-emitting double-layer micro-fluidic chip and detection system

Technical Field

The utility model belongs to the technical field of the luminous immunodetection of micro-fluidic chip, especially, relate to a luminous double-deck micro-fluidic chip of magnetic particle and detecting system.

Background

Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital; the other type of miniaturization and bedside miniaturization is realized, namely, the rapid analysis and diagnosis on site is realized through handheld small simple equipment. However, small hospitals are not capital intensive, have a small sample size, and are not suitable for purchasing expensive large equipment. Therefore, most of the rapid detection equipment adopted by hospitals at the present stage mainly comprises test strips and corollary equipment thereof, but the test strips can only realize qualitative or semi-quantitative detection, and have the advantages of low detection sensitivity, poor specificity, poor repeatability and obvious interference. Due to the fact that China has a large population, aging is aggravated, the disease incidence is increased sharply, and dependence on large hospitals is overwhelmed. Therefore, the development of a rapid detection method and equipment which are simple and convenient to operate, high in sensitivity, good in repeatability and accurate in quantification becomes urgent.

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has been developed into a research field with multiple interdisciplinary disciplines of biology, chemistry, medicine, fluid, material, machinery, etc., and is applied to the fields of biomedical research, biochemical detection, judicial appraisal, etc. However, the existing microfluidic chip is provided with a top plate and a bottom plate, when a sample flows from the top plate to the bottom plate, the sample can automatically flow backwards along the channel due to the gravity action because the channels of the bottom plate are at the same level. In some detection items, detection and analysis can be completed only by taking a small amount of sample, and if the sample flows from the top plate to the bottom plate and directly flows in the bottom plate, the final detection result is greatly influenced, and the detection result is wrong. In addition, the sample flows through the channel of the bottom plate, the flow path of the sample cannot be limited, the sample flowing to the detection area cannot be completely filtered, and the final detection result is further influenced.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a luminous double-deck micro-fluidic chip of magnetic particle and detecting system aims at solving current sample among the micro-fluidic chip and directly flows in the bottom plate after roof flow direction bottom plate to can't inject the problem of the route of flowing through of sample.

The embodiment of the utility model provides a realize like this, provide a luminous double-deck micro-fluidic chip of magnetic particle, the chip includes: the top plate comprises an adding part, a labeled ligand storage part and a sample mixing region, wherein a labeled ligand is arranged in the labeled ligand storage part, and the sample mixing region is communicated with the adding part and the labeled ligand storage part respectively; the bottom plate is arranged on the top plate and comprises a flow guide area communicated with the sample mixing area, a magnetic particle coating part communicated with the flow guide area, a cleaning area communicated with the magnetic particle coating part, a detection area communicated with the cleaning area and a cleaning solution storage part communicated with the cleaning area, a groove lower than the bottom wall of the magnetic particle coating part, a flow guide part arranged on the groove and connected with the magnetic particle coating part, a cutting groove arranged below the front end of the flow guide part and a blocking part arranged on the flow guide part are arranged in the flow guide area, a magnetic particle ligand solution is arranged in the magnetic particle coating part, and a cleaning solution is arranged in the cleaning solution storage part.

Furthermore, the top plate also comprises an air pump which is mutually communicated with the sample adding part.

Furthermore, the top plate is provided with an elastic member at a position corresponding to the air pump and the sample mixing area.

Further, a porous elastic member is provided inside the air pump.

Furthermore, the sample adding part comprises a sample adding port and a sealing cover for opening or sealing the sample adding port, and the sample adding part also comprises a rubber ring arranged on the sample adding port.

Furthermore, the top plate and the bottom plate are provided with limiting notches at corresponding positions.

Furthermore, a first buckle or a first clamping groove is arranged on the top plate, a second clamping groove or a second buckle is arranged on the bottom plate, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle.

Furthermore, a part or all of the area of at least one side of the bottom plate is provided with a single-sided adhesive substance.

Furthermore, a product label is arranged on the surface of the top plate or the bottom plate, and a two-dimensional code label is arranged on the surface of the top plate or the bottom plate.

Furthermore, the top plate is provided with magnetic attraction yielding holes on corresponding communication tracks of the magnetic particle coating part, the cleaning area and the detection area.

Further, the bottom plate further comprises a waste liquid pool communicated with the cleaning area.

Furthermore, the bottom plate further comprises a luminous liquid storage part which is communicated with the detection area, and luminous liquid is arranged in the luminous liquid storage part.

Furthermore, the top plate is provided with a cleaning liquid relief hole and a luminous liquid relief hole at corresponding positions of the cleaning liquid storage part and the luminous liquid storage part.

Further, a fluorescent liquid is provided inside the labeled ligand storage part.

The utility model also provides a luminous double-deck micro-fluidic detecting system of magnetic particle, detecting system includes: the magnetic particle light-emitting double-layer micro-fluidic chip is described above; the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move; a squeezing unit for squeezing the labeled ligand storage part and the cleaning solution storage part to make the labeled ligand and the cleaning solution flow out; a detection unit for detecting a luminescence signal in the detection zone.

The beneficial effects of the utility model reside in that, compared with the prior art, the utility model discloses a design a luminous double-deck micro-fluidic chip of magnetic particle and detecting system, the sample gets into from application of sample portion, mix district and mark ligand intermix at the sample, the recess in the diversion district gets into again, because the recess is less than magnetic particle coating portion diapire, need just to suck away the sample in the recess through capillary action, and because the effect of truncation groove and blocking portion, the sample can only get into magnetic particle coating portion from diversion portion, again with the abundant mixed reaction of magnetic particle ligand, and after the washing that obtains the washing liquid in the purge zone, realize luminous the detection at the detection zone.

Drawings

Fig. 1 is an exploded schematic view of a magnetic particle light-emitting double-layer microfluidic chip according to an embodiment of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1;

fig. 3 is another exploded schematic view of a magnetic particle light-emitting double-layer microfluidic chip according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The utility model discloses a design a luminous double-deck micro-fluidic chip of magnetic particle and detecting system, the sample gets into from application of sample portion 11, mix each other at sample mixing area 13 and mark ligand, reentrant guide flow area 21's recess 211, because recess 211 is less than magnetic particle peridium portion 22 diapire, need just can siphon away the sample in the recess 211 through capillary action, the sample gets into magnetic particle peridium portion 22 from guide flow portion 212, again with the abundant mixed reaction of magnetic particle ligand, and after the washing that obtains the washing liquid in washing area 23, realize luminous the detection in detection zone 24.

Example one

Referring to fig. 1 and 2, the present embodiment provides a magnetic particle light-emitting double-layer microfluidic chip, which includes a top plate 1 and a bottom plate 2 disposed on the top plate 1.

The top plate 1 includes an addition part 11, a labeled ligand storage part 12, and a sample mixing region 13, wherein a labeled ligand is provided inside the labeled ligand storage part 12, and the sample mixing region 13 is communicated with the addition part 11 and the labeled ligand storage part 12, respectively.

The base plate 2 includes a flow guide region 21 communicating with the sample mixing region 13, a magnetic particle coating portion 22 communicating with the flow guide region 21, a washing region 23 communicating with the magnetic particle coating portion 22, a detection region 24 communicating with the washing region 23, a washing liquid storage portion 25 communicating with the washing region 23, the inside of the flow guide area 21 is provided with a groove 211 which is lower than the bottom wall of the magnetic particle coating part 22, a flow guide part 212 which is arranged on the groove 211 and is connected with the magnetic particle coating part 22, a cut-off groove 213 which is arranged below the front end of the flow guide part 212 and a blocking part 214 which is arranged on the flow guide part 212, the flow guide part 212 can be selected from blood filtering membranes, the blocking part 214 can be selected from plastic paper, the bottom wall of the cut-off groove 213 is also provided with blood filtering membranes, the height of the cut-off groove 213 is lower than that of the groove 211, the inside of the magnetic particle coating part 22 is provided with a magnetic particle ligand solution, and the inside of the cleaning solution storage.

For example, the sample is a whole blood sample, the sample to be tested is placed in the sample adding portion 11, the sample enters the sample mixing region 13 through the sample adding portion 11, the labeled ligand in the labeled ligand storage portion 12 also enters the sample mixing region 13, the sample and the labeled ligand are mixed in the sample mixing region 13, and then the sample enters the flow guide portion 212 of the flow guide region 21 from the sample mixing region 13. After passing through the flow guiding region 21, plasma in the sample is separated from blood cells, the plasma enters the magnetic particle coating portion 22 from the flow guiding portion 212, and the blood cells remain in the flow guiding region 21. Since the groove 211 of the flow guiding region 21 is lower than the bottom wall of the magnetic particle coating portion 22, and similarly, the height of the flow guiding portion 212 is lower than the bottom wall of the magnetic particle coating portion 22, after the sample enters the flow guiding region 21, the sample will not automatically enter the magnetic particle coating portion 22 from the flow guiding portion 212 due to gravity, but will be sucked away from the flow guiding portion 212 by capillary action, so that a smaller volume of sample which can meet the detection requirement can be sucked away from a larger volume of sample, and the detection result is prevented from being influenced by a larger amount of sample. Moreover, when the sample is introduced into the flow guide region 21, the sample can only overflow from the blocking portion 214 due to the action of the blocking groove 213 and the blocking portion 214, and the sample which does not flow through the flow guide portion 212 can fall into the blocking groove 213, so that the sample can only flow into the capillary channel of the magnetic particle coating portion 22 from the flow guide portion 212, the flow area of the sample flowing into the magnetic particle coating portion 22 is limited, and the sample does not flow out of the flow guide portion 212, so that the detection sample flows into the capillary channel of the magnetic particle coating portion 22 from other areas, and therefore, the filtering effect is better, and the detection result is more accurate. It should be noted that, if the sample to be detected is whole blood, the red blood cells do not diffuse into the capillary channel of the magnetic particle coating portion 22 from the front end of the flow guide portion 212, but are subjected to the capillary action of the capillary channel, so as to adsorb the filtered blood plasma into the magnetic particle coating portion 22.

After the guided sample reaches the corresponding position of the magnetic particle coating part 22, the analyte in the sample reacts with the magnetic particle ligand solution, the external magnet collects the magnetic particles in the magnetic particle ligand solution, the reacted sample enters the cleaning area 23, and the external magnet also drives the magnetic particles to enter the cleaning area 23. At this time, the cleaning solution in the cleaning solution storage portion 25 is released, the cleaning solution enters the cleaning region 23 to clean the magnetic particles in the sample, the sample enters the detection region 24 from the cleaning region 23, and the external magnet also drives the magnetic particles to enter the detection region 24, so that the quantitative detection of the analyte in the sample is realized.

Wherein, the blood filtering membrane is preset in the flow guiding area 21, wherein the blood filtering membrane can separate the liquid from the cells through the physical aperture or the biological/chemical reagent, so as to realize the separation of the blood plasma from the red blood cells, the blood plasma flows to the magnetic particle coating part 22, and the red blood cells stay on the blood filtering membrane, thereby reducing the interference of the red blood cells on the test result. The biological/chemical reagent contains coagulant and the like, which can connect among red blood cells to form clot and increase the size, and the red blood cells after increasing the size are blocked by the reticular structure of the blood filtering membrane more easily, thereby more effectively reducing the interference of the red blood cells to the experimental result.

A washing liquid for washing the magnetic beads to remove the non-specifically adsorbed analyte, the luminescent agent label, and other substances that affect the detection result is stored in the washing liquid storage portion 25 in advance. The cleaning solution mainly comprises a buffer reagent, protein and a surfactant, wherein the buffer reagent comprises but is not limited to borate, phosphate, Tris-HCl, acetate and the like, and the pH range of the cleaning solution is 6.0-10.0. The protein includes but is not limited to bovine serum albumin, casein, etc. Wherein the surfactant includes, but is not limited to, Tween 20, Tween 80, Triton X-100, polyethylene glycol, polyvinylpyrrolidone, etc. Preferably, in this embodiment, the washing solution is Tris-HCl buffer (pH7.0) containing bovine serum albumin, Tween 20 and Proclin 300.

In this embodiment, the labeled ligand storage part 12, the magnetic particle coating part 22 and the cleaning solution storage part 25 are sealed cavities, and the sealing material is made of an elastic material or a high-barrier film, specifically, glass, plastic, rubber, aluminum foil or a high-barrier film, wherein the sealing material may be made of the same material or a combination of multiple materials. Under physical compression, the tag ligand storage portion 12, the magnetic particle-coated portion 22, and the cleaning liquid storage portion 25 may be locally ruptured, thereby releasing the stored material.

Example two

Referring to fig. 1, in addition to the first embodiment, the top plate 1 of the fourth embodiment is further provided with an air pump 14 communicated with the sample adding part 11, the air pump 14 is an air bag built in the top plate 1, and the air in the air bag is repeatedly pushed or released to repeatedly move the air in the air bag into and out of the sample adding part 11 and the sample mixing region 13, so as to drive the liquid in the top plate 1 to flow.

The air pump 14 is used to absorb or squeeze air in the sample-mixing zone 13, to flow the sample and the labeled ligand to the sample-mixing zone 13, and to sufficiently mix the sample and the labeled ligand in the sample-mixing zone 13 by repeatedly absorbing or squeezing air in the top plate 1, and after mixing, to drive the sample from the sample-mixing zone 13 into the flow guide zone 21.

It should be noted that, because the air pump 14 needs to be operated in a sealed environment, in order to seal the chip interior, the cover needs to be closed after the sample is added into the sample addition port, so as to seal the chip interior, and ensure the driving effect of the air pump 14.

EXAMPLE III

Referring to fig. 1, on the basis of the second embodiment, the top plate 1 of the third embodiment is provided with elastic members at the air pump 14 and the sample mixing area 13. The elastic member may be optionally adhered to one side of the top plate 1, and the one side of the top plate 1 is adjacent to the bottom plate 2. The elastic member may provide an elastic function to the air pump 14 and the sample mixing zone 13.

Example four

Referring to fig. 1, on the basis of the second embodiment, a porous elastic member is arranged inside the air pump 14 of the fourth embodiment, and the porous elastic member can be selected as a sponge. The porous elastic member may provide a resilient force to the pump surface of the air pump 14 to return the pump surface to a taut state, requiring repeated compression of the air pump 14 and removal of the compression force after compression of the air pump 14. And the porous elastic member is provided with a plurality of orifices, so that the porous elastic member can reduce the volume inside the air pump 14, contributing to further miniaturization of the air pump 14.

EXAMPLE five

Referring to fig. 1, in the first embodiment, the sample adding part 11 of the fifth embodiment includes a sample adding port and a cover for opening or closing the sample adding port.

The exterior can load a sample into the sample port when the lid is open, and after loading the sample, the lid is closed to close the sample port.

In detail, the sealing cover is provided with a first clamping piece or a first clamping hole, a second clamping piece or a second clamping piece is arranged at a position adjacent to the sample adding port, and the first clamping piece and the second clamping hole are mutually matched or the first clamping hole and the second clamping piece are mutually matched so that the sealing cover closes the sample adding port. And, still be equipped with the involution that suits with the sample addition mouth on the closing cap, when the closing cap was closed, the involution was inserted the sample addition mouth simultaneously, avoided the sample to spill in the sample addition mouth.

And, sample addition part 11 is still including establishing the rubber circle on the sample addition mouth, because the outside adds the sample through the pipette tip usually, and the rubber circle has elasticity, helps sealing with the pipette tip to inject the sample into from the sample addition mouth more smoothly.

EXAMPLE six

On the basis of the first embodiment, the top plate 1 and the bottom plate 2 of the sixth embodiment are provided with limiting notches at corresponding positions. The limit notch is a notch provided at one end of the top plate 1 and the bottom plate 2, and is not shown in the figure. In the detection process of the magnetic particle light-emitting double-layer micro-fluidic chip, in order to avoid the magnetic particle light-emitting double-layer micro-fluidic chip from deviating, the magnetic particle light-emitting double-layer micro-fluidic chip needs to be limited. Spacing breach and outside limit structure mutually support, can firmly fix the chip in the testing process, avoid its condition of taking place the skew to guarantee going on smoothly of detecting.

EXAMPLE seven

Referring to fig. 1, on the basis of the first embodiment, a first buckle or a first clamping groove is arranged on the top plate 1, and a second clamping groove or a second buckle is arranged on the bottom plate 2, so that the top plate 1 and the bottom plate 2 are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle. Through foretell lock mode, can make roof 1 and bottom plate 2 can dismantle the setting, be favorable to operating personnel to inspect or change. After the top plate 1 and the bottom plate 2 are fastened, the top plate 1 and the bottom plate 2 can be firmly fixed with each other.

Of course, the top plate 1 and the bottom plate 2 may be combined with each other in other manners, which are not described in detail herein.

Example eight

Referring to fig. 3, on the basis of the first embodiment, in the eighth embodiment, a single-sided adhesive substance 3 is disposed on a part or all of a region of at least one side of the bottom plate 2, and the single-sided adhesive substance 3 may be a single-sided adhesive tape. Preferably, both sides of the bottom plate 2 are provided with single-sided viscous substances 3, and the single-sided viscous substances 3 have a sealing effect on the bottom plate 2. In the present embodiment, the single-sided viscous substance 3 is provided with a relief area at a corresponding position of the cleaning liquid storage portion.

Example nine

Referring to fig. 1, on the basis of the first embodiment, a product label is disposed on a surface of a top plate 1 of the ninth embodiment, and related descriptions of a chip, such as a chip name and a detection target of the chip, are provided on the product label.

And the surface of the top plate 1 or the bottom plate 2 is provided with a two-dimensional code label, and a camera on an external detection instrument can scan the two-dimensional code on the two-dimensional code label so as to read corresponding data, such as the name of a chip, a detection target object of the chip, product calibration information and the like. The set position of the two-dimensional code tag depends on the orientation position of the camera of the external detection device.

Example ten

Referring to fig. 1, on the basis of the first embodiment, the top plate 1 of the tenth embodiment is provided with magnetic attraction yielding holes 15 on the corresponding communication tracks with the magnetic particle coating portion 22, the cleaning area 23 and the detection area 24. The external magnet moves along the set direction of the magnetic attraction abdicating hole 15, and drives the magnetic particles to move along the magnetic particle coating part 22, the cleaning area 23 and the detection area 24 in sequence. And, be equipped with magnetism and inhale abdicating hole 15, magnet can be close to bottom plate 2 more, avoids interval roof 1 between bottom plate 2 and the magnet, improves the reliability of magnetism greatly.

EXAMPLE eleven

Referring to fig. 1, on the basis of the first embodiment, the bottom plate 2 of the eleventh embodiment is provided with the waste liquid tank 27 communicated with the cleaning area 23, and the waste liquid tank 27 can collect the waste liquid after cleaning and reaction, so that the interference of the waste liquid on the final detection can be reduced, and the detection accuracy can be effectively improved. The waste liquid tank 27 may be provided in plurality.

Example twelve

Referring to fig. 1, in addition to the first to eleventh embodiments, the bottom plate 2 of the twelfth embodiment further includes a light-emitting liquid storage portion 26 communicating with the detection area 24, and the light-emitting liquid is disposed inside the light-emitting liquid storage portion 26.

The sample enters the detection area 24 after being cleaned in the cleaning area 23, at this time, the luminous liquid in the luminous liquid storage part 26 is released, the luminous liquid and the sample generate luminous reaction to send out a luminous signal, an external detection instrument detects the intensity of the luminous signal,

the light emitting liquid is stored in the light emitting liquid storage unit 26 in advance, and the light emitting liquid is used for further washing the magnetic beads or enhancing the light emitting signal. The luminous liquid comprises substrate liquid and luminous enhancement liquid, wherein the substrate liquid can be acid solution containing luminol or acid solution containing adamantane, and the luminous enhancement liquid can be alkaline solution containing benzene derivatives.

It should be noted that, considering that the substrate solution and the luminescence enhancement solution are not suitable for long-term mixing and storage, the luminescence solution storage portion 26 may be provided with a first luminescence solution storage portion in which the substrate solution is stored and a second luminescence solution storage portion in which the luminescence enhancement solution is stored, and a luminescence solution mixing region is provided on the bottom plate 2, and the luminescence solution mixing region is respectively communicated with the detection region 24, the first luminescence solution storage portion and the second luminescence solution storage portion. When the first luminescence liquid storage part and the second luminescence liquid storage part release, the substrate liquid and the luminescence enhancement liquid enter the luminescence liquid mixing region and are mixed with each other, and then enter the detection region 24 after being uniformly mixed.

The luminous liquid storage part 26 is a sealed cavity, and the sealing material is made of elastic material or high-barrier film, specifically glass, plastic, rubber, aluminum foil or high-barrier film, wherein the sealing material can be made of the same material or a combination of multiple materials. Under physical pressure, the luminous liquid storage portion 26 may be locally ruptured, thereby releasing the stored luminous liquid.

The labeled ligand is stored in advance in the labeled ligand storage portion 12, and when the labeled ligand includes an enzyme-labeled ligand, the enzyme may be selected from one or more of horseradish peroxide and alkaline phosphatase, and the ligand may be selected from one or more of an antigen, an antibody, a hapten and a nucleic acid. A magnetic particle ligand solution is pre-stored in the magnetic particle coating 22, the magnetic particle ligand solution including magnetic particles including but not limited to iron sesquioxide and iron oxide ferricompound, sugars, buffer reagents, proteins, surfactants, and preservatives.

Where the labeled ligand comprises an enzyme-labeled ligand, the enzyme binds or competes with the analyte in the sample to form the enzyme-labeled ligand; the magnetic particle label binds or competes with the analyte in the sample to form a magnetic bead labeled ligand, which may be the same or different; magnetically labeled ligands, enzyme labeled ligands include nucleic acids, antigens, monoclonal antibodies, polyclonal antibodies, and hormone receptors, and analytes in a sample include DNA, small molecules (drugs or drugs), antigens, antibodies, hormones, antibiotics, bacteria or viruses, and other biochemical markers.

In this embodiment, the labeled ligand may be bound to a solution of magnetic particle ligand (e.g., a double antibody sandwich), or the labeled ligand may compete with the labeled ligand (e.g., a competition method). Wherein the enzyme-labeled ligand can be the same as or different from the magnetic particle ligand solution. Preferably, in one embodiment of the invention, the analyte is detected in a double antibody sandwich method by selecting two different antibodies as the labeled ligand and the magnetic particle ligand solution. In another embodiment of the present invention, an antigen and an antibody are selected as the labeled ligand and the magnetic particle ligand solution, respectively, to detect the analyte by a competitive method.

EXAMPLE thirteen

Referring to fig. 1, in addition to the twelfth embodiment, in the top plate 1 of the thirteenth embodiment, a cleaning liquid relief hole and a light emitting liquid relief hole are formed in corresponding positions of the cleaning liquid storage portion 25 and the light emitting liquid storage portion 26 on the bottom plate 2.

Since the cleaning liquid storage portion 25 and the light emitting liquid storage portion 26 are always provided with a certain shape, such as a cylinder, in order to adapt to the shapes of the cleaning liquid storage portion 25 and the light emitting liquid storage portion 26 and avoid the chip from locally protruding, a cleaning liquid relief hole and a light emitting liquid relief hole are provided on the top plate 1.

In the present embodiment, the cleaning liquid receding hole and the light emitting liquid receding hole together constitute the receding hole 16.

Example fourteen

Referring to fig. 1, on the basis of the first to eleventh embodiments, a fluorescent agent is disposed inside the labeled ligand storage portion 12 of the fourteenth embodiment. That is, the labeled ligand includes a fluorescent agent labeled ligand, the fluorescent agent may be selected from one or more of acridinium ester, ABEI, a fluorescent dye, a fluorescent protein and a fluorescent microsphere, and the ligand may be selected from one or more of an antigen, an antibody, a hapten and a nucleic acid. Wherein the acridinium ester and the ABEI can directly emit light after being reacted with the luminous liquid; fluorescent dyes, fluorescent proteins and fluorescent microspheres require excitation light sources, but do not require luminescent liquids.

When the labeled ligand comprises a fluorescent agent labeled ligand, the fluorescent agent binds or competes with the analyte in the sample to form a fluorescent agent labeled ligand; the magnetic particle ligand solution binds or competes with the analyte in the sample to form a magnetic bead labeled ligand, and the two ligands can be the same or different; the magnetic particle ligand solution, the fluorescent agent labeled ligand comprise nucleic acids, antigens, monoclonal antibodies, polyclonal antibodies and hormone receptors, and the analytes in the sample comprise DNA, small molecules (drugs or drugs), antigens, antibodies, hormones, antibiotics, bacteria or viruses and other biochemical markers.

In this embodiment, the labeled ligand may be bound to a solution of magnetic particle ligand (e.g., a double antibody sandwich), or the labeled ligand may compete with a solution of magnetic particle ligand (e.g., a competition method). Wherein the fluorescent agent-labeled ligand may be the same as or different from the magnetic particle ligand solution. Preferably, in one embodiment of the invention, the analyte is detected in a double antibody sandwich method by selecting two different antibodies as the labeled ligand and the magnetic particle ligand solution.

Example fifteen

This embodiment fifteen provides a magnetic particle luminescence double-deck micro-fluidic detecting system, and detecting system includes: the magnetic particle light-emitting double-layer microfluidic chip according to the first embodiment to the fourteenth embodiment; the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move; a pressing unit for crushing the labeled ligand storage part 12 and the cleaning solution storage part 25 to make the labeled ligand and the cleaning solution flow out; a detection unit for detecting the sample in the detection zone 24.

The magnet unit comprises a magnet and a driving part for driving the magnet to move, the driving part can be a linear motor, and an output shaft of the linear motor is fixedly connected with the magnet. After the linear motor is started, an output shaft of the linear motor stretches out to drive the magnet to move, and the magnet adsorbs magnetic particles to drive the magnetic particles to move.

The pressing unit may be a linear motor, and after the linear motor is started, an output shaft thereof extends to press the tag ligand storage part 12 and the cleaning solution storage part 25, so that the tag ligand and the cleaning solution flow out, respectively. Of course, the output shaft of the linear motor may be fixedly provided with one or more extrusion parts, the extrusion parts are matched with the storage parts in terms of state and size, and the extrusion parts are driven by the output shaft of the linear motor to move, and then extrude the labeled ligand storage part 12 and the cleaning solution storage part 25, so that the labeled ligand and the cleaning solution flow out respectively.

The detection unit can be selected from a photodiode, a photomultiplier or an avalanche photodiode, a sample enters the detection area after being mixed and reacted through the process, the mixed sample has a luminous signal, the detection unit collects the luminous signal, and the detection result of the sample is obtained according to the intensity of the luminous signal.

When the magnetic particle light-emitting double-layer micro-fluidic chip is provided with the air pump 14, the extrusion unit can also be used for extruding the air pump 14, so that the liquid on the top plate 1 is driven to flow.

When the magnetic particle light-emitting double-layer micro-fluidic chip is provided with the light-emitting liquid storage part 26, the extrusion unit can also be used for breaking the light-emitting liquid storage part 26 so as to enable the light-emitting liquid to flow out.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims

1. A magnetic particle light-emitting double-layer micro-fluidic chip is characterized by comprising:

the top plate comprises an adding part, a labeled ligand storage part and a sample mixing region, wherein a labeled ligand is arranged in the labeled ligand storage part, and the sample mixing region is communicated with the adding part and the labeled ligand storage part respectively;

the bottom plate is arranged on the top plate and comprises a flow guide area communicated with the sample mixing area, a magnetic particle coating part communicated with the flow guide area, a cleaning area communicated with the magnetic particle coating part, a detection area communicated with the cleaning area and a cleaning solution storage part communicated with the cleaning area, a groove lower than the bottom wall of the magnetic particle coating part, a flow guide part arranged on the groove and connected with the magnetic particle coating part, a cutting groove arranged below the front end of the flow guide part and a blocking part arranged on the flow guide part are arranged in the flow guide area, a magnetic particle ligand solution is arranged in the magnetic particle coating part, and a cleaning solution is arranged in the cleaning solution storage part.

2. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate further comprises an air pump communicated with the sample adding part.

3. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein the top plate is provided with an elastic member at a position corresponding to the air pump and the sample mixing region.

4. The magnetic particle light-emitting double-layer microfluidic chip according to claim 2, wherein a porous elastic member is disposed inside the air pump.

5. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring arranged on the sample application port.

6. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate and the bottom plate are provided with limiting notches at positions corresponding to each other.

7. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein the top plate is provided with a first buckle or a first buckle, the bottom plate is provided with a second buckle or a second buckle, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second buckle or the mutual matching of the first buckle and the second buckle.

8. The magnetic particle light-emitting double-layer microfluidic chip according to claim 1, wherein a single-sided adhesive substance is provided on a part or all of at least one side of the bottom plate.

9. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein a product label is disposed on the surface of the top plate or the bottom plate, and a two-dimensional code label is disposed on the surface of the top plate or the bottom plate.

10. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the top plate is provided with magnetic attraction yielding holes on corresponding communication tracks with the magnetic particle coating part, the cleaning area and the detection area.

11. The magnetic particle light-emitting double-layer microfluidic chip of claim 1, wherein the base plate further comprises a waste reservoir in communication with the cleaning region.

12. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein the bottom plate further comprises a light-emitting liquid storage portion communicated with the detection region, and a light-emitting liquid is disposed inside the light-emitting liquid storage portion.

13. The magnetic particle light-emitting double-layer microfluidic chip according to claim 12, wherein the top plate is provided with a cleaning solution relief hole and a light-emitting solution relief hole at corresponding positions of the cleaning solution storage part and the light-emitting solution storage part.

14. The magnetic particle light-emitting double-layer microfluidic chip according to any one of claims 1 to 11, wherein a fluorescent liquid is provided inside the labeled ligand storage part.

15. A magnetic particle light emitting dual layer microfluidic detection system, the detection system comprising:

the magnetic particle light-emitting double-layer microfluidic chip of any one of claims 1 to 14;

the magnet unit is used for driving the magnetic particles in the magnetic particle ligand solution to move;

a squeezing unit for squeezing the labeled ligand storage part and the cleaning solution storage part to make the labeled ligand and the cleaning solution flow out;

a detection unit for detecting a luminescence signal in the detection zone.