CN211374779U - Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device - Google Patents

Abstract

The utility model belongs to the technical field of the luminous immunodetection of micro-fluidic chip, especially, relate to a magnetic particle luminous micro-fluidic chip and detection device that many markers detected, include: the top plate is provided with a sample adding part, a mixing area and a plurality of marking ligands arranged in the mixing area; the bottom plate comprises a flow guide area, a plurality of reaction areas, a plurality of detection areas which are mutually communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are mutually communicated with the detection areas; each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part stores luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area. Because the reaction zone is provided with a plurality of reaction zones, the phenomenon of cross interference among magnetic particles can not occur, the accuracy of the detection result can be greatly improved, the moving speed of the magnet is not required, and the control of the device is favorably realized.

Description

Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device

Technical Field

The utility model belongs to the technical field of the luminous immunodetection of micro-fluidic chip, especially, relate to a magnetic particle luminous micro-fluidic chip and detection device that many markers detected.

Background

Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital; the other type of miniaturization and bedside miniaturization is realized, namely, the rapid analysis and diagnosis on site is realized through handheld small simple equipment. However, small hospitals are not capital intensive, have a small sample size, and are not suitable for purchasing expensive large equipment. Therefore, most of the rapid detection equipment adopted by hospitals at the present stage mainly comprises test strips and corollary equipment thereof, but the test strips can only realize qualitative or semi-quantitative detection, and have the advantages of low detection sensitivity, poor specificity, poor repeatability and obvious interference. Due to the fact that China has a large population, aging is aggravated, the disease incidence is increased sharply, and dependence on large hospitals is overwhelmed. Therefore, the development of a rapid detection method and equipment which are simple and convenient to operate, high in sensitivity, good in repeatability and accurate in quantification becomes urgent.

Chemiluminescence refers to the phenomenon in which chemical energy is converted into light energy by reaction intermediates, reaction products or an additional luminescent reagent in a chemical reaction process. Compared with fluorescence and absorption light, chemiluminescence has no interference of external excitation light source background signals, has small cross interference, and has the advantages of high sensitivity, wide linear range and the like. The chemiluminescence analysis established by the method is widely applied to the fields of clinical diagnosis and the like. The chemiluminescence apparatus is a main large-scale IVD analysis and detection device.

The micro-fluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in the biological, chemical and medical analysis process into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine, etc., it has been developed into a research field with multiple interdisciplinary disciplines of biology, chemistry, medicine, fluid, material, machinery, etc., and is applied to the fields of biomedical research, biochemical detection, judicial appraisal, etc.

For the existing microfluidic chip, when the labeled ligand required by the sample is different, the sample enters a reaction area reacted with the magnetic particle ligand after being mixed with the labeled ligand, and then enters a detection area for quantitatively analyzing and detecting the sample after the reaction. However, all the magnetic particle ligands are placed in the same reaction region, and because the magnetic particle volumes, weights and nuclear-to-mass ratios of different magnetic particle ligands are different, different magnetic particles can be driven to move sequentially by controlling the moving speed of the external magnet, so that the different magnetic particles enter the detection region. Although the moving speed of the magnetic particles is different, especially under the condition that the moving speed of the magnet is higher, the stroke of the magnetic particles is shorter, the phenomenon that the magnetic particles are mutually crossed and interfered is still easily caused, and different labeled ligand solutions are mixed, so that the problem of poor stability exists in the mixing of different magnetic particle ligands, and the accuracy of a detection result is greatly reduced.

SUMMERY OF THE UTILITY MODEL

The embodiment of the utility model provides a luminous micro-fluidic chip of magnetic particle and detection device that many markers detected aims at solving the luminous micro-fluidic chip of current magnetic particle and when examining, and the easy intercrossing of magnetic particle disturbs, and stability is relatively poor, the problem of greatly reduced testing result accuracy.

The embodiment of the utility model provides a realize like this, provide a magnetic particle luminous micro-fluidic chip that many markers detected, include: the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another; the bottom plate comprises a flow guide area communicated with the mixing area, a plurality of reaction areas communicated with the flow guide area, a plurality of detection areas communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are communicated with the detection areas; each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different; the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid; and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.

Further, the mixing region is provided with a labeled ligand storage part, and the labeled ligand is stored in the labeled ligand storage part.

Further, the number of the sample addition parts and the number of the labeled ligand storing parts are equal to each other.

Furthermore, the sample adding part comprises a sample adding port and a sealing cover for opening or sealing the sample adding port, and the sample adding part also comprises a rubber ring arranged on the sample adding port.

Furthermore, a first buckle or a first clamping groove is arranged on the top plate, a second clamping groove or a second buckle is arranged on the bottom plate, and the top plate and the bottom plate are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle.

Furthermore, the top plate is provided with a magnetic attraction abdicating hole on the corresponding communication track of the reaction area and the detection area.

Furthermore, the top plate is provided with a cleaning liquid relief hole and a luminous liquid relief hole at corresponding positions of the cleaning liquid storage part and the luminous liquid storage part.

Furthermore, a groove with the height lower than the bottom wall of the reaction area and a flow guide part which is arranged on the groove and connected with the reaction area are arranged inside the flow guide area.

Furthermore, the detection area comprises a plurality of cleaning areas and a plurality of light emitting areas which are communicated with each other, and the reaction area, the cleaning areas and the light emitting areas are arranged in a one-to-one correspondence manner.

The utility model also provides a magnetic particle luminous micro-fluidic detection device that many markers detected, include: the magnetic particle light-emitting microfluidic chip as described above; the magnet unit is used for driving the magnetic particles to move; a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump; a detection unit for detecting a luminescence signal in the detection zone.

Compared with the prior art, the utility model provides a magnetic particle luminous micro-fluidic chip and a detection device for multi-marker detection, wherein a sample enters from the sample adding part of a top plate, the sample and each labeled ligand are mixed in a mixing area and then enter a flow guide area, and enters different reaction zones of the bottom plate from the diversion zone and enters different detection zones from the reaction zones, at the moment, the external magnet drives the magnetic particles of the magnetic particle ligand to enter different detection zones, the luminous detection is realized in the detection area, and because a plurality of reaction areas are arranged, the phenomenon of cross interference among magnetic particles can not occur, and the condition of mixing different labeled ligand solutions can not occur, the stability is good, the accuracy of the detection result can be greatly improved, the moving speed of the magnet is not required, and the control of the device is favorably realized.

Drawings

Fig. 1 is a schematic diagram of a magnetic particle luminescent microfluidic chip provided with a plurality of labeled ligand storage portions, a reaction region, a cleaning region and a luminescent region according to an embodiment of the present invention;

fig. 2 is a schematic view of a magnetic particle luminescent microfluidic chip provided with a plurality of sample adding portions, a labeled ligand storage portion, a reaction region, a cleaning region and a luminescent region according to an embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

The utility model provides a luminous micro-fluidic chip of magnetic particle and detection device that many markers detected, the sample gets into from the application of sample portion 11 of roof 1, the sample is mixed each other at mixing zone 12 with each mark ligand, reentrant guiding zone 21, and get into bottom plate 2's different reaction zone 22 from guiding zone 21, and get into different detection zones from reaction zone 22, at this moment, outside magnet drives the magnetic particle of magnetic particle ligand and gets into different detection zones, realize luminous the detection at the detection zone, because reaction zone 22 is equipped with a plurality ofly, each magnetic particle can not take place cross interference's phenomenon each other, and can not take place the mixed condition of different mark ligand solutions, stability is better, can improve the accuracy of testing result greatly, and also do not have the requirement to the moving speed of magnet, be favorable to realizing device's control.

Example one

The embodiment provides a magnetic particle luminescence microfluidic chip for multi-marker detection, which comprises: a top plate 1, wherein the top plate 1 is provided with at least one sample adding part 11, a mixing region 12 mutually communicated with the sample adding part 11, and a plurality of labeling ligands arranged in the mixing region 12, and the labeling ligands are different from one another; a base plate 2, the base plate 2 including a flow guide region 21 communicating with the mixing region 12, a plurality of reaction regions 22 communicating with the flow guide region 21, a plurality of detection regions communicating with the corresponding reaction regions 22, a cleaning solution storage part 24 and a luminescent solution storage part 25 communicating with the detection regions; each reaction zone 22 is provided with a magnetic particle ligand, the ligands of each magnetic particle ligand being different; a cleaning liquid is provided in the cleaning liquid storage portion 24, and a luminescent liquid is stored in the luminescent liquid storage portion 25; and an air pump 13 disposed on the top plate 1 for driving the sample in the sample adding part 11 to flow through the mixing region 12, wherein the air pump 13 can be selected from an air bag built in the top plate 1.

For example, the sample is a whole blood sample, the sample to be tested is placed in the sample adding portion 11, the air pump 13 is squeezed, and the sample enters the mixing region 12 through the sample adding portion 11 and is mixed with the labeled ligands in the mixing region 12. After mixing, the sample enters the flow guiding region 21 from the mixing region 12, a blood filtering membrane is disposed on the flow guiding region 21, the plasma in the sample is separated from the blood cells, the plasma enters each reaction region 22 from the flow guiding region 21 and is mixed with the magnetic particle ligand in each reaction region 22, and the blood cells remain in the flow guiding region 21.

After the sample is mixed with the magnetic particle ligands, the sample enters each detection region from each reaction region 22, at this time, the external magnet drives the magnetic particles of the magnetic particle ligands to enter each detection region from each reaction region 22, and the ligands of the magnetic particle ligands in each reaction region 22 are different, but there is no requirement for the magnetic particles, which may be the same or different, depending on the specific detection items. At the same time, the external magnet drives the magnetic particles in each reaction zone 22 into the detection zone, and the magnetic particle ligands also enter the detection zone. Subsequently, the cleaning liquid storage portion 24 discharges the cleaning liquid stored inside, and the cleaning liquid enters each detection region to perform cleaning of the magnetic particles. Then, the luminescent liquid storage portion 25 releases the luminescent liquid stored therein, and the luminescent liquid enters each detection region, thereby realizing quantitative detection of the analyte in the sample. Since the magnetic particles are respectively distributed in different reaction regions 22, the phenomenon of cross interference among the magnetic particles does not occur, and the accuracy of the detection result can be greatly improved. In addition, because the magnetic particles are respectively distributed in different reaction areas 22, the magnetic particles do not need to be controlled to move into a detection area successively, the moving speed of the magnet is not required, and the control of the device is favorably realized.

Wherein, the blood filtering membrane is preset in the flow guiding region 21, wherein the blood filtering membrane can separate the liquid from the cells through the physical aperture or the biological/chemical reagent, so as to realize the separation of the blood plasma from the red blood cells, the blood plasma flows to the reaction region 22, and the red blood cells stay on the blood filtering membrane, thereby reducing the interference of the red blood cells on the test result. The biological/chemical reagent contains coagulant and the like, which can connect among red blood cells to form clot and increase the size, and the red blood cells after increasing the size are blocked by the reticular structure of the blood filtering membrane more easily, thereby more effectively reducing the interference of the red blood cells to the experimental result.

A washing liquid for washing the magnetic particles and removing the non-specifically adsorbed analyte, luminescent agent label, and other substances that affect the detection result is stored in the washing liquid storage portion 24 in advance. The cleaning solution mainly comprises a buffer reagent, protein and a surfactant, wherein the buffer reagent comprises but is not limited to borate, phosphate, Tris-HCl, acetate and the like, and the pH range of the cleaning solution is 6.0-10.0. The protein includes but is not limited to bovine serum albumin, casein, etc. Wherein the surfactant includes, but is not limited to, Tween 20, Tween 80, Triton X-100, polyethylene glycol, polyvinylpyrrolidone, etc. Preferably, in this embodiment, the washing solution is Tris-HCl buffer solution (pH7.0) containing bovine serum albumin, Tween 20 and Proclin 300.

The light emitting liquid is stored in the light emitting liquid storage portion 25 in advance, and the light emitting liquid is used to further wash the magnetic particles or enhance the light emitting signal. The luminous liquid comprises substrate liquid and luminous enhancement liquid, wherein the substrate liquid can be acid solution containing luminol or acid solution containing adamantane, and the luminous enhancement liquid can be alkaline solution containing benzene derivatives.

It is worth mentioning that, considering that the substrate solution and the luminescence enhancement solution are not suitable for long-term mixing and storage, the luminescence solution storage part 25 may be provided with a first luminescence solution storage part 25 and a second luminescence solution storage part 25, the substrate solution is stored in the first luminescence solution storage part 25, the luminescence enhancement solution is stored in the second luminescence solution storage part 25, the bottom plate 2 is provided with a luminescence solution mixing zone 12, and the luminescence solution mixing zone 12 is respectively communicated with the detection zone, the first luminescence solution storage part 25 and the second luminescence solution storage part 25. When the first luminescent liquid reservoir part 25 and the second luminescent liquid reservoir part 25 are released, the substrate liquid and the luminescence enhancement liquid enter the luminescent liquid mixing region 12 and are mixed with each other, and then enter the detection region after being uniformly mixed.

In the present embodiment, the cleaning liquid storage portion 24 and the light emitting liquid storage portion 25 are sealed cavities, and the sealing material used is an elastic material or a high-barrier film, specifically, glass, plastic, rubber, aluminum foil or a high-barrier film, wherein the sealing material may be composed of the same material or a combination of multiple materials. Under physical pressure, the cleaning liquid storage portion 24 and the light emitting liquid storage portion 25 may be locally ruptured, thereby releasing the stored materials.

Example two

Referring to fig. 1 and 2, in addition to the first embodiment, the mixing region 12 of the second embodiment is provided with a labeled ligand storage part 14, and the labeled ligand is stored in the labeled ligand storage part 14. Since the labeled ligand is stored in advance in the labeled ligand storage part 14, long-term storage of the labeled ligand is facilitated, and deterioration of the labeled ligand is avoided. In addition, different labeled ligands can be stored in the different labeled ligand storage parts 14, the enzyme labeling ratios required for different detection items can be controlled, and the different labeled ligands can be prevented from affecting each other during storage.

Where the labeled ligand comprises an enzyme-labeled ligand, the enzyme may be selected from one or more of horseradish peroxide and alkaline phosphatase, and the ligand may be selected from one or more of an antigen, an antibody, a hapten and a nucleic acid. A magnetic particle ligand solution is pre-stored in the magnetic particle coating 22, the magnetic particle ligand solution including magnetic particles including but not limited to iron sesquioxide and iron oxide ferricompound, sugars, buffer reagents, proteins, surfactants, and preservatives.

Where the labeled ligand comprises an enzyme-labeled ligand, the enzyme binds or competes with the analyte in the sample to form the enzyme-labeled ligand; the magnetic particle label binds or competes with the analyte in the sample to form a magnetic particle labeled ligand, which may be the same or different; magnetically labeled ligands, enzyme labeled ligands include nucleic acids, antigens, monoclonal antibodies, polyclonal antibodies, and hormone receptors, and analytes in a sample include DNA, small molecules (drugs or drugs), antigens, antibodies, hormones, antibiotics, bacteria or viruses, and other biochemical markers.

In this embodiment, the labeled ligand may be bound to a solution of magnetic particle ligand (e.g., a double antibody sandwich), or the labeled ligand may compete with the labeled ligand (e.g., a competition method). Wherein the enzyme-labeled ligand can be the same as or different from the magnetic particle ligand solution. Preferably, in one embodiment of the invention, the analyte is detected in a double antibody sandwich method by selecting two different antibodies as the labeled ligand and the magnetic particle ligand solution. In another embodiment of the present invention, an antigen and an antibody are selected as the labeled ligand and the magnetic particle ligand solution, respectively, to detect the analyte by a competitive method.

EXAMPLE III

Referring to fig. 2, in the second embodiment, the number of the sample addition parts 11 and the number of the labeled ligand storage parts 14 in the third embodiment are the same, and each sample addition part 11 and each labeled ligand storage part 14 are arranged correspondingly, and the two parts are in a one-to-one correspondence relationship. In this way, the enzyme labeling ratios required for different detection items can be controlled, and the required samples can also be controlled.

Example four

In addition to the first embodiment, the sample addition part 11 of the fourth embodiment includes a sample addition port and a cover for opening or closing the sample addition port.

The exterior can load a sample into the sample port when the lid is open, and after loading the sample, the lid is closed to close the sample port.

In detail, the sealing cover is provided with a first clamping piece or a first clamping hole, a second clamping piece or a second clamping piece is arranged at a position adjacent to the sample adding port, and the first clamping piece and the second clamping hole are mutually matched or the first clamping hole and the second clamping piece are mutually matched so that the sealing cover closes the sample adding port. And, still be equipped with the involution that suits with the sample addition mouth on the closing cap, when the closing cap was closed, the involution was inserted the sample addition mouth simultaneously, avoided the sample to spill in the sample addition mouth.

And, sample addition part 11 is still including establishing the rubber circle on the sample addition mouth, because the outside adds the sample through the pipette tip usually, and the rubber circle has elasticity, helps sealing with the pipette tip to inject the sample into from the sample addition mouth more smoothly.

EXAMPLE five

On the basis of the first embodiment, the top plate 1 of the fifth embodiment is provided with a first buckle or a first clamping groove, and the bottom plate 2 is provided with a second clamping groove or a second buckle, so that the top plate 1 and the bottom plate 2 are buckled with each other through the mutual matching of the first buckle and the second clamping groove or the mutual matching of the first clamping groove and the second buckle. Through foretell lock mode, can make roof 1 and bottom plate 2 can dismantle the setting, be favorable to operating personnel to inspect or change. After the fastening, the top plate 11 and the bottom plate 22 can be firmly fixed to each other.

Of course, the top plate 1 and the bottom plate 2 may be combined with each other in other manners, which are not described in detail herein.

EXAMPLE six

Referring to fig. 1 and 2, on the basis of the first embodiment, the top plate 1 of the sixth embodiment is provided with magnetic attraction holes 15 on the corresponding communication tracks with the reaction area 22 and the detection area.

The top plate 1 is provided with a magnetic attraction abdicating hole 15 on the corresponding communication track with the reaction area 22 and the detection area. The external magnet moves along the set direction of the magnetic attraction yielding hole 15, and drives the magnetic particles to move along the reaction area 22 and the detection area in sequence. And, be equipped with magnetism and inhale abdicating hole 15, magnet can be close to bottom plate 2 more, avoids interval roof 1 between bottom plate 2 and the magnet, improves the reliability of magnetism greatly.

EXAMPLE seven

In addition to the first embodiment, in the top plate 1 of the seventh embodiment, a cleaning liquid relief hole and a light emitting liquid relief hole are provided in the bottom plate 2 at positions corresponding to the cleaning liquid storage portion 24 and the light emitting liquid storage portion 25.

Since the cleaning liquid storage portion 24 and the light emitting liquid storage portion 25 are always provided with a certain shape, such as a cylinder, and in order to adapt to the shapes of the cleaning liquid storage portion 24 and the light emitting liquid storage portion 25 and prevent the chip from locally protruding, a cleaning liquid relief hole and a light emitting liquid relief hole are provided on the top plate 1.

In the present embodiment, referring to fig. 1 and 2, the cleaning liquid receding hole and the light emitting liquid receding hole together constitute a receding hole 16.

Example eight

On the basis of the first embodiment, the flow guiding region 21 of the eighth embodiment is internally provided with a groove with a height lower than the bottom wall of the reaction region 22 and a flow guiding part arranged on the groove and connected with the reaction region 22, and the flow guiding part can be a blood filtering membrane. Since the groove of the flow guide region 21 is lower than the bottom wall of the reaction region 22, and similarly, the height of the flow guide portion is lower than the bottom wall of the reaction region 22, after the sample enters the flow guide region 21, the sample will not automatically enter the reaction region 22 from the flow guide portion due to gravity, but will be sucked away from the flow guide portion by capillary action, so that a small volume of sample which can meet the detection requirement can be sucked away from a large volume of sample, and the influence of a large amount of sample on the detection result can be avoided.

Example nine

Referring to fig. 1 and 2, on the basis of the first to eighth embodiments, the detection area of the eighth embodiment includes a plurality of cleaning areas 231 and a plurality of light emitting areas 232 which are communicated with each other, the plurality of cleaning areas 231 is provided, the plurality of light emitting areas 232 is provided, and the reaction areas 22, the cleaning areas 231 and the light emitting areas 232 are all the same in number and are arranged in a one-to-one correspondence with each other. The guiding region 21, the reaction region 22, the cleaning region 231 and the light emitting region 232 are sequentially connected. The sample gets into each reaction zone 22 from water conservancy diversion district 21, with the magnetic particle ligand intermix in the reaction zone 22, get into the cleaning zone 231 from the reaction zone 22 again, get into each detection zone from the cleaning zone 231 at last, outside magnet drives the magnetic particle of magnetic particle ligand and gets into different cleaning zones 231 from different reaction zones 22 simultaneously, at this moment, cleaning solution storage portion 24 releases the inside washing liquid of saving, the washing liquid flows into the cleaning zone 231, wash the magnetic particle, different magnetic particles get into different detection zones from the cleaning zone 231 again afterwards. At the detection zone, the sample may then be subjected to analytical testing. If only be equipped with a washing district 231, there is the risk of cross interference when concentrating the washing to magnetic particle, consequently, be equipped with a plurality of washing districts 231 to washing district 231 corresponds the setting with luminous zone 232, and the magnetic particle can get into corresponding luminous zone 232 after the washing, thereby has further improved the accuracy of testing result.

Example ten

This embodiment provides a magnetic particle luminescence microfluidic detection device that many markers detected, includes: the magnetic particle light-emitting microfluidic chip according to any one of embodiments one to nine; the magnet unit is used for driving the magnetic particles to move; a pressing unit for pressing the cleaning liquid storage part 24, the light emitting liquid storage part 25 and the air pump 13; a detection unit for detecting a luminescence signal in the detection zone.

The magnet unit comprises a magnet and a driving part for driving the magnet to move, the driving part can be a linear motor, and an output shaft of the linear motor is fixedly connected with the magnet. After the linear motor is started, an output shaft of the linear motor stretches out to drive the magnet to move, and the magnet adsorbs magnetic particles to drive the magnetic particles to move.

Wherein, the extrusion unit can be selected as a linear motor, after the linear motor is started, the output shaft of the linear motor extends out to break the cleaning liquid storage part 24 and the luminous liquid storage part 25, so that the cleaning liquid and the luminous liquid respectively flow out. And after the linear motor is started, the output shaft which extends and retracts in a reciprocating manner can repeatedly extrude the air pump 13, so that the liquid on the top plate 1 is driven to flow. Of course, the output shaft of the linear motor may be fixedly provided with one or more extrusion parts, the extrusion parts are matched with the storage parts in terms of state and size, and the extrusion parts are driven by the output shaft of the linear motor to move, and then extrude the cleaning liquid storage part 24, the luminous liquid storage part 25 and the air pump 13.

The detection unit can be selected from a photodiode, a photomultiplier or an avalanche photodiode, a sample enters the detection area after being mixed and reacted through the process, the mixed sample has a luminous signal, the detection unit collects the luminous signal, and the detection result of the sample is obtained according to the intensity of the luminous signal.

When the magnetic particle light-emitting double-layer microfluidic chip is provided with the labeled ligand storage part 14, the extrusion unit can also be used for crushing the labeled ligand storage part 14 so as to make the labeled ligand flow out.

Wherein the magnetic particle ligands can be encoded for subsequent accurate determination of the entry of magnetic particles in different magnetic particle ligands into different detection zones.

The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.

Claims (10)

1. A magnetic particle luminous micro-fluidic chip for multi-marker detection is characterized by comprising:

the top plate is provided with at least one sample adding part, a mixing region communicated with the sample adding part and a plurality of labeling ligands arranged in the mixing region, and the labeling ligands are different from one another;

the bottom plate comprises a flow guide area communicated with the mixing area, a plurality of reaction areas communicated with the flow guide area, a plurality of detection areas communicated with the corresponding reaction areas, a cleaning liquid storage part and a luminous liquid storage part which are communicated with the detection areas;

each reaction zone is provided with a magnetic particle ligand, and the ligands of the magnetic particle ligands are different;

the cleaning liquid storage part is internally provided with cleaning liquid, and the luminous liquid storage part is internally provided with luminous liquid;

and the air pump is arranged on the top plate and used for driving the sample in the sample adding part to flow through the mixing area.

2. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the mixing region is provided with a labeled ligand storage portion, and the labeled ligand is stored in the labeled ligand storage portion.

3. The magnetic particle luminescent microfluidic chip of claim 2, wherein the number of the sample addition parts and the number of the labeled ligand storage parts are consistent with each other.

4. The magnetic particle luminescent microfluidic chip of claim 1, wherein the sample application part comprises a sample application port and a cover for opening or closing the sample application port, and the sample application part further comprises a rubber ring disposed on the sample application port.

5. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the top plate is provided with a first snap or a first snap, and the bottom plate is provided with a second snap or a second snap, so that the top plate and the bottom plate are fastened to each other by the cooperation of the first snap and the second snap, or the cooperation of the first snap and the second snap.

6. The magnetic particle luminescent microfluidic chip of claim 1, wherein the top plate has magnetically attractive relief holes on corresponding communication tracks with the reaction zone and the detection zone.

7. The magnetic particle luminescent microfluidic chip according to claim 1, wherein the top plate is provided with a cleaning solution relief hole and a luminescent solution relief hole at corresponding positions of the cleaning solution storage part and the luminescent solution storage part.

8. The magnetic particle luminescent microfluidic chip of claim 1, wherein the flow guide region has a groove therein with a height lower than the bottom wall of the reaction region and a flow guide portion disposed on the groove and connected to the reaction region.

9. The magnetic particle luminescent microfluidic chip according to any one of claims 1 to 8, wherein the detection region comprises a plurality of washing regions and a plurality of luminescent regions, which are communicated with each other, and the reaction region, the washing region and the luminescent regions are arranged in one-to-one correspondence.

10. A multi-marker detection magnetic particle luminescence microfluidic detection device, comprising:

the magnetic particle luminescent microfluidic chip of any one of claims 1 to 9;

a magnet unit for moving the magnetic particles;

a pressing unit for pressing the cleaning liquid storage part, the luminous liquid storage part and the air pump;

a detection unit for detecting a luminescence signal in the detection zone.

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