CN210752736U - Liquid storage structure for micro-fluidic chip and micro-fluidic chip - Google Patents
Liquid storage structure for micro-fluidic chip and micro-fluidic chip Download PDFInfo
- Publication number
- CN210752736U CN210752736U CN201920986737.3U CN201920986737U CN210752736U CN 210752736 U CN210752736 U CN 210752736U CN 201920986737 U CN201920986737 U CN 201920986737U CN 210752736 U CN210752736 U CN 210752736U
- Authority
- CN
- China
- Prior art keywords
- liquid
- cavity
- micro
- storage structure
- opening
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model is suitable for a micro-fluidic chip luminescence immunoassay technical field provides a liquid storage structure and micro-fluidic chip for micro-fluidic chip, the storage structure includes the cavity, the top of cavity is provided with first opening, and the side is provided with the second opening; a liquid bag for storing liquid is arranged in the cavity; the second opening is connected with a channel; and a puncturing piece is arranged in the channel, and the liquid sac is punctured by the puncturing piece when the liquid sac is extruded by external force. The utility model can effectively store the liquid by arranging the liquid bag for storing the liquid in the cavity, avoid the contact of the liquid with the cavity or other parts, reduce the possibility of deterioration of the liquid and improve the stability of the liquid performance, thereby effectively improving the detection accuracy of the micro-fluidic chip; when the liquid bag is used, the liquid bag is punctured through the puncturing piece, so that liquid flows out, and flows to the channel from the second opening, and the liquid bag is simple, convenient and fast.
Description
Technical Field
The invention belongs to the technical field of micro-fluidic chip luminescence immunoassay, and particularly relates to a liquid storage structure for a micro-fluidic chip and the micro-fluidic chip.
Background
Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital, and the adopted reagent is a large-package reagent and can be used for analyzing samples for many times; the other miniaturized and bedside analyzer adopts single-person packaged reagent to realize on-site rapid analysis and diagnosis.
The small hospital or community hospital has insufficient funds and small sample amount, is not suitable for purchasing expensive large-scale equipment, has few samples to be analyzed, and has limited service time after the large packaged reagent is unpacked, so that the reagent is overdue and wasted. The miniaturized analyzer uses a single-person packaged reagent, and can solve the problems of high cost and reagent waste of large-scale equipment in small hospitals or community hospitals.
The microfluidic chip is also called a Lab-on-a-chip (Lab-on-a-chip), and is characterized in that basic operation units related to the fields of biology, chemistry, medicine and the like, such as sample preparation, reaction, separation, detection and the like, are integrated on a chip with a micro-channel with a micron scale, and the whole process of reaction and analysis is automatically completed. The analysis and detection device based on the microfluidic chip has the advantages that: the sample dosage is less, the analysis speed is fast, the portable instrument is convenient to manufacture, and the method is very suitable for real-time and on-site analysis.
Although various components are integrally arranged on the existing single-person packaged microfluidic chip, the liquid components cannot be well stored, so that the liquid components are easy to deteriorate, and the test result is deviated.
Disclosure of Invention
The embodiment of the invention provides a magnetic particle light-emitting micro-fluidic chip, aiming at solving the problem that liquid components of the traditional micro-fluidic chip cannot be well stored.
The embodiment of the invention is realized by providing a liquid storage structure for a microfluidic chip, wherein the storage structure comprises a cavity, the top of the cavity is provided with a first opening, and the side surface of the cavity is provided with a second opening;
a liquid bag for storing liquid is arranged in the cavity;
the second opening is connected with a channel;
a puncturing piece is arranged in the channel, and the liquid sac is punctured by the puncturing piece when the liquid sac is extruded by external force;
the first opening is provided with an elastic body for sealing the first opening.
Further, the bottom of the cavity is connected to the channel.
Furthermore, at least one of the channel and the cavity adopts a micro-channel structure, and at least one dimension of the micro-channel is a micrometer scale.
Still further, the piercing member includes a main body and a piercing portion, and the sac is pierced by the piercing portion when the sac is pressed by an external force.
Still further, the piercing member is provided with a central through hole.
Furthermore, a flow passage is arranged at the end part of the needling part.
The embodiment of the invention also provides a microfluidic chip, which comprises:
a substrate;
a liquid labeled ligand;
the liquid storage structure disposed on the substrate, the liquid-labeled ligand being disposed in a sac of the liquid storage structure.
The micro-fluidic chip has the advantages that the liquid bag for storing the liquid is arranged in the cavity, so that the liquid can be effectively stored, the liquid is prevented from contacting the cavity or other parts, the possibility of deterioration of the liquid can be reduced, the stability of the liquid performance is improved, and the detection accuracy of the micro-fluidic chip can be effectively improved; when the liquid bag is used, the liquid bag is punctured through the puncturing piece, so that liquid flows out, and flows to the channel from the second opening, and the liquid bag is simple, convenient and fast.
Drawings
Fig. 1 is a partial cross-sectional view of a magnetic particle light-emitting microfluidic chip provided in an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
According to the invention, the liquid bag for storing liquid is arranged in the cavity, so that the liquid in the liquid bag can be led out only by pressing the liquid bag to the puncture part during detection, and the liquid can be channeled.
Example one
As shown in fig. 1, the embodiment of the present invention is achieved by providing a liquid storage structure for a microfluidic chip, wherein the liquid storage structure comprises a cavity 11, a first opening is arranged at the top of the cavity, and a second opening 112 is arranged at the side of the cavity;
a liquid bag 12 for storing liquid is arranged in the cavity 11;
the second opening 112 is connected with a channel 2;
a puncture piece 3 is arranged in the channel 2, and when the liquid sac 12 is extruded by external force, the liquid sac 12 is punctured by the puncture piece 3;
an elastic body 111 for sealing the first opening is arranged at the first opening.
Specifically, the deformable material of the elastic body 111 is an elastic material or a high-barrier film, specifically, a plastic, a rubber, an aluminum foil or a high-barrier film, wherein the sealing material may be composed of the same material or a combination of multiple materials. The elastic body 111 seals the cavity 11 to form a closed space, and prevents the liquid from overflowing from the first opening after the liquid bag 12 is broken.
Specifically, the sac 12 may be completely disposed in the cavity 11, or a portion of the sac 12 may extend from the first opening to be exposed to the cavity 11.
According to the invention, the liquid bag 12 for storing liquid is arranged in the cavity 11, so that the liquid can be effectively stored, the liquid is prevented from contacting with the cavity or other parts, the possibility of deterioration of the liquid can be reduced, the stability of the liquid performance is improved, and the detection accuracy of the microfluidic chip can be effectively improved; when the liquid bag is used, the liquid bag 12 is pressed from the first opening, the liquid bag 12 is deformed, the liquid bag 12 is made to contact with the puncturing piece 3, the puncturing piece 3 punctures the liquid bag 12, and liquid flows out of the liquid bag and flows to the channel 2 from the second opening 112.
Example two
In an alternative embodiment of the invention, the bottom of the cavity 11 is connected to the channel 2. The liquid magnetic bead labeled ligand flows out through the channel 2, and the channel 2 is arranged at the bottom of the cavity 11, so that the liquid magnetic bead labeled ligand cannot be retained in the cavity 11, and the liquid magnetic bead labeled ligand can flow out conveniently.
EXAMPLE III
In an alternative embodiment of the present invention, at least one of the trench 2 and the cavity 11 is in a microchannel 2 structure, and at least one dimension of the microchannel 2 is in a micrometer scale.
The micro-fluidic chip integrates and embeds all reagent components (sample liquid, enzyme-labeled ligand, magnetic bead labeled ligand, cleaning liquid, luminescent liquid and the like) required in the detection process into the micro-fluidic chip, and realizes one-click operation of the micro-fluidic chip (detection can be realized only by pressing a start key without complex operation) under the operation of a matched instrument through the ingenious micro-channel 2 design, so that the whole blood separation, the immunoreaction, the cleaning separation and the chemiluminescence detection are realized, and the defects and defects of simple structural design, complex operation during detection and the like in the conventional micro-fluidic chip are overcome. And the defect that the traditional chemiluminescence apparatus can only detect serum or plasma but not a whole blood sample is overcome.
Example four
In an alternative embodiment of the present invention, the puncturing member 3 comprises a main body 31 and a puncturing portion 32, and the sac 12 is punctured by the puncturing portion 32 when the sac 12 is pressed by an external force. Specifically, the needle-punched portion 32 is in the shape of an elongated cone or other sharp shape, and the end of the needle-punched portion 32 abuts against the sac 12; the liquid bag 12 can be effectively and quickly punctured by the puncturing part 32, so that the liquid in the liquid bag 12 can flow out conveniently.
EXAMPLE five
In an alternative embodiment of the invention, said piercing member 3 is provided with a through-going hole. The middle through hole is arranged, so that liquid in the liquid bag 12 can flow out along the middle through hole conveniently, a flow guide effect can be achieved, and the liquid bag is simple and convenient to use.
EXAMPLE six
In an alternative embodiment of the present invention, the end of the needle punching portion 32 is provided with a flow passage. The flow path may be provided on the outer wall of the needle portion 32, or a through hole penetrating the puncture tool 3 may be opened from the end of the needle portion 32. The flow channel is arranged, so that liquid in the liquid bag 12 can flow out along the flow channel conveniently, the flow guide effect can be achieved, and the liquid bag is simple and convenient to use.
EXAMPLE seven
The embodiment of the invention also provides a microfluidic chip, which comprises:
a substrate;
a liquid labeled ligand;
the liquid storage structure disposed on the substrate, the liquid-labeled ligand being disposed in a sac of the liquid storage structure.
According to the invention, the liquid bag for storing the liquid is arranged in the cavity, so that the liquid can be effectively stored, the liquid is prevented from contacting with the cavity or other parts, the possibility of deterioration of the liquid can be reduced, the stability of the liquid performance is improved, and the detection accuracy of the microfluidic chip can be effectively improved; when the liquid bag puncture device is used, the liquid bag is pressed from the first opening, so that the liquid bag deforms, the liquid bag is in contact with the puncture piece, the puncture piece punctures the liquid bag, liquid flows out, and the liquid flows to the channel 2 from the second opening, and the liquid bag puncture device is simple, convenient and fast.
Example eight
In the microfluidic chip provided by the embodiment of the invention, the liquid labeling ligand comprises a magnetic bead labeling ligand and/or an enzyme labeling ligand.
The magnetic bead labeling ligand is a liquid homogeneous phase magnetic labeling ligand, and the liquid homogeneous phase magnetic labeling ligand comprises magnetic beads, a temperature sensitive material and a solution, wherein the magnetic beads comprise one or more of carboxyl magnetic beads, amino magnetic beads, streptavidin magnetic beads, antibody modified magnetic beads and antigen modified magnetic beads; the temperature sensitive material is a thermoreversible gel comprising one or more of gelatin, agar, alginate, carrageenan, hydroxymethyl cellulose, acacia, guar gum, locust bean gum, pectin, starch, and xanthan gum; the solution is a buffer system containing surfactant and protein. Because the liquidity of the liquid is good, the liquid homogeneous phase magnetic labeling ligand, the enzyme labeling ligand and the added sample are fully mixed during detection, the reaction speed can be effectively improved, and the detection sensitivity, repeatability and accuracy are improved.
The enzyme-labeled ligand comprises an enzyme-or luminescent agent-labeled ligand.
The enzyme includes: one or more of horseradish peroxide and alkaline phosphatase; the luminescent agent includes: one or more of acridinium ester, ABEI, fluorescent dye or fluorescent microsphere.
Specifically, the ligands include: one or more of an antigen, an antibody, a hapten and a nucleic acid.
The enzyme or luminescent agent binds or competes with the analyte to form an enzyme or luminescent agent labeled ligand; the magnetic particle label binds or competes with the analyte to form a magnetic bead labeled ligand, which may be the same or different; the ligand used for labeling the ligand by the magnetic enzyme labeling ligand, the enzyme or the luminescent agent comprises nucleic acid, antigen, monoclonal antibody, polyclonal antibody and hormone receptor, and the analyte comprises DNA, small molecules (drugs or drugs), antigen, antibody, hormone, antibiotic, bacteria or virus and other biochemical markers.
In this embodiment, the enzyme-labeled ligand may be bound to the magnetic bead-labeled ligand (e.g., double antibody sandwich) or may compete with the magnetic bead-labeled ligand (e.g., competition). The ligand marked by the luminescent agent and the ligand marked by the enzyme can be the same as or different from the ligand marked by the magnetic beads. Preferably, in one embodiment of the invention, the analyte is detected in a double antibody sandwich method by selecting two different antibodies as the enzyme-labeled ligand and the magnetic bead-labeled ligand. In another embodiment of the present invention, an antigen and an antibody are selected as the enzyme-labeled ligand and the magnetic bead-labeled ligand, respectively, to detect the sample by a competitive method.
The invention is not simply superposed with the magnetic particle chemiluminescence technology and the microfluidic chip technology, but integrates and embeds all chemical components required by detection into the microfluidic chip through liquid seal design and channel 2 design, and realizes one-key type magnetic particle chemiluminescence immunoassay by actively driving with a magnet, thereby realizing rapid, high-sensitivity and accurate quantitative detection of analytes in whole blood in a portable matched instrument.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. A liquid storage structure for a microfluidic chip is characterized by comprising a cavity, wherein the top of the cavity is provided with a first opening, and the side surface of the cavity is provided with a second opening;
a liquid bag for storing liquid is arranged in the cavity;
the second opening is connected with a channel;
a puncturing piece is arranged in the channel, and the liquid sac is punctured by the puncturing piece when the liquid sac is extruded by external force;
the first opening is provided with an elastic body for sealing the first opening.
2. The liquid storage structure for a microfluidic chip according to claim 1, wherein the bottom of the cavity is connected to the channel.
3. The liquid storage structure for a microfluidic chip according to claim 1, wherein at least one of said channel and said cavity has a microchannel structure, and at least one dimension of said microchannel is a micrometer scale.
4. The liquid storage structure for the microfluidic chip according to any one of claims 1 to 3, wherein the puncturing member comprises a main body and a puncturing part, and the liquid bag is punctured by the puncturing part when being pressed by an external force.
5. The liquid storage structure for a microfluidic chip according to claim 4, wherein said piercing member is provided with a through-hole.
6. The liquid storage structure for a microfluidic chip according to claim 4, wherein the end of the needle-punched portion is provided with a flow channel.
7. A microfluidic chip, wherein the chip comprises:
a substrate;
a liquid labeled ligand;
the fluid storage structure of any of claims 1-6 disposed on the substrate, the fluid-marking ligand being disposed in a sac of the fluid storage structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920986737.3U CN210752736U (en) | 2019-06-27 | 2019-06-27 | Liquid storage structure for micro-fluidic chip and micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920986737.3U CN210752736U (en) | 2019-06-27 | 2019-06-27 | Liquid storage structure for micro-fluidic chip and micro-fluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210752736U true CN210752736U (en) | 2020-06-16 |
Family
ID=71057309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920986737.3U Active CN210752736U (en) | 2019-06-27 | 2019-06-27 | Liquid storage structure for micro-fluidic chip and micro-fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210752736U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114100702A (en) * | 2020-08-27 | 2022-03-01 | 京东方科技集团股份有限公司 | Detection chip, preparation method, use method and detection device thereof |
| CN114308144A (en) * | 2020-09-28 | 2022-04-12 | 北京京东方健康科技有限公司 | Micro-fluidic chip |
-
2019
- 2019-06-27 CN CN201920986737.3U patent/CN210752736U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114100702A (en) * | 2020-08-27 | 2022-03-01 | 京东方科技集团股份有限公司 | Detection chip, preparation method, use method and detection device thereof |
| CN114100702B (en) * | 2020-08-27 | 2023-05-30 | 京东方科技集团股份有限公司 | Detection chip, preparation method, use method and detection device thereof |
| CN114308144A (en) * | 2020-09-28 | 2022-04-12 | 北京京东方健康科技有限公司 | Micro-fluidic chip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110252434B (en) | Liquid storage structure for micro-fluidic chip and micro-fluidic chip | |
| US11026611B2 (en) | Rotatable disk-shaped fluid sample collection device | |
| US7122153B2 (en) | Self-contained microfluidic biochip and apparatus | |
| US20220184618A1 (en) | Magnetic particle luminescent micro-fluidic chip for multi-marker detection and detection apparatus | |
| CN109212201A (en) | A kind of centrifugal type microfludic chip for hepatitis B five detections in serum | |
| US20220184619A1 (en) | Magnetic particle luminescent micro-fluidic chip for multi-marker detection and detection apparatus | |
| US20220357320A1 (en) | Two-layer microfluidic chip with magnetic bead luminescence and detection system | |
| CN210752736U (en) | Liquid storage structure for micro-fluidic chip and micro-fluidic chip | |
| CN109425733A (en) | Flexible biological probe, its application method and application apparatus | |
| CN210720414U (en) | Magnetic particle luminous micro-fluidic chip | |
| CN211374779U (en) | Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device | |
| CN102998472B (en) | Automatic device for heterogeneous immunization rapid analysis and use method of automatic device | |
| CN210954058U (en) | Magnetic bead releasing mechanism and system | |
| CN210720415U (en) | Active liquid flow control micro-fluidic detection system | |
| CN211603208U (en) | Multi-marker detection magnetic particle luminous micro-fluidic chip and detection device | |
| CN207472894U (en) | Flexible biological probe | |
| CN210720417U (en) | Active liquid flow control micro-fluidic detection system | |
| CN110208521A (en) | A kind of magnetic particle shines micro-fluidic chip and reaction method | |
| CN211179850U (en) | Magnetic particle light-emitting double-layer micro-fluidic chip and detection system | |
| CN111624085B (en) | Sample diluting device and reagent card for detecting novel coronavirus antibody | |
| KR102244375B1 (en) | Apparatus for detecting analyte by simultaneous movement of particles and solution and detection method using the same | |
| CN210720416U (en) | Magnetic particle luminous micro-fluidic chip | |
| CN210787392U (en) | Magnetic particle luminous micro-fluidic chip | |
| CN210720418U (en) | Magnetic particle luminous micro-fluidic chip | |
| JP4283112B2 (en) | Test equipment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |