CN210954058U - Magnetic bead releasing mechanism and system - Google Patents
Magnetic bead releasing mechanism and system Download PDFInfo
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- CN210954058U CN210954058U CN201921005003.9U CN201921005003U CN210954058U CN 210954058 U CN210954058 U CN 210954058U CN 201921005003 U CN201921005003 U CN 201921005003U CN 210954058 U CN210954058 U CN 210954058U
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- magnetic bead
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- electromagnet
- microfluidic chip
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Abstract
The utility model is suitable for a luminous immunodetection technical field provides a magnetic bead releasing mechanism and system, the mechanism includes: a base for placing the microfluidic chip; an electromagnet; the base is provided with a placing groove for placing the microfluidic chip, and the electromagnet is arranged at a preset position below the placing groove. The utility model discloses an the electro-magnet will preset position is collected to the magnetic bead in the liquid magnetic bead mark distribution, during the use, cuts off the power of electro-magnet, makes the electro-magnet lose magnetic force to release the magnetic bead, make the magnetic bead evenly flow out and all release along with the solution in the liquid magnetic bead mark distribution, can effectively improve the accuracy that detects.
Description
Technical Field
The invention belongs to the technical field of micro-fluidic chip luminescence immunoassay, and particularly relates to a magnetic bead release mechanism and a magnetic bead release system.
Background
Currently, there are two major trends In Vitro Diagnostics (IVD): one is automatic and integrated, namely, the high-precision disease analysis and diagnosis is realized by utilizing full-automatic and high-sensitivity large-scale instruments and equipment of a central laboratory matched with a large-scale hospital, and the adopted reagent is a large-package reagent and can be used for analyzing samples for many times; the other miniaturized and bedside analyzer adopts single-person packaged reagent to realize on-site rapid analysis and diagnosis.
The small hospital or community hospital has insufficient funds and small sample amount, is not suitable for purchasing expensive large-scale equipment, has few samples to be analyzed, and has limited service time after the large packaged reagent is unpacked, so that the reagent is overdue and wasted. The miniaturized analyzer uses a single-person packaged reagent, and can solve the problems of high cost and reagent waste of large-scale equipment in small hospitals or community hospitals.
The microfluidic chip is also called a Lab-on-a-chip (Lab-on-a-chip), and is characterized in that basic operation units related to the fields of biology, chemistry, medicine and the like, such as sample preparation, reaction, separation, detection and the like, are integrated on a chip with a micro-channel with a micron scale, and the whole process of reaction and analysis is automatically completed. The analysis and detection device based on the microfluidic chip has the advantages that: the sample dosage is less, the analysis speed is fast, the portable instrument is convenient to manufacture, and the method is very suitable for real-time and on-site analysis.
However, when the existing single-person packaged microfluidic chip is used for detection, when the liquid magnetic bead labeled ligand is placed, the magnetic beads contained in the microfluidic chip can be precipitated, the positions of the precipitated magnetic beads are related to the placing direction of a user, the outflow quantity of the magnetic beads cannot be controlled during use, the detection result is influenced, and the test result is deviated.
Disclosure of Invention
The embodiment of the invention provides a magnetic bead release mechanism and a magnetic bead release system, and aims to solve the technical problems that magnetic beads of an existing microfluidic chip are easy to precipitate and cannot be released completely when the existing microfluidic chip is used.
The embodiment of the present invention is implemented as follows, and provides a magnetic bead release mechanism, including:
a base for placing the microfluidic chip;
an electromagnet;
the base is provided with a placing groove for placing the microfluidic chip, and the electromagnet is arranged at a preset position below the placing groove.
The embodiment of the invention also provides a magnetic bead release system, which comprises the magnetic bead release mechanism and a micro-fluidic chip arranged on the magnetic bead release mechanism;
the microfluidic chip is placed in the placing groove of the base;
the electromagnet collects the magnetic beads in the liquid magnetic bead marking ligand to a preset position, the magnetic beads are released when the electromagnet is used, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead marking ligand.
Still further, the microfluidic chip comprises:
a substrate;
labeling a ligand by liquid magnetic beads;
a storage part arranged on the substrate and used for storing liquid magnetic bead labeled ligands;
the storage part is provided with a channel arranged on the substrate, and a breakable spacer is arranged at the joint of the storage part and the channel;
and under the external force extrusion of the storage part, the breakable septum is broken, and the liquid magnetic bead labeled ligand flows out of the storage part.
Further, the bottom of the memory portion is connected to the channel.
Furthermore, at least one of the channel and the storage part adopts a micro-channel structure, and at least one dimension of the micro-channel is a micrometer scale.
Furthermore, the liquid magnetic bead labeled ligand comprises a magnetic bead, a temperature sensitive material and a solution.
The invention has the beneficial effects that the magnetic beads in the liquid magnetic bead labeled ligand are collected to the preset position through the electromagnet, and when the device is used, the power supply of the electromagnet is cut off, so that the electromagnet loses the magnetic force, and the magnetic beads are released, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead labeled ligand and are completely released; the detection accuracy can be effectively improved.
Drawings
FIG. 1 is a perspective view of one embodiment of a magnetic bead release mechanism provided by embodiments of the present invention;
FIG. 2 is a perspective view of an embodiment of a magnetic bead releasing system according to an embodiment of the present invention;
FIG. 3 is a perspective view of another embodiment of a magnetic bead releasing system according to an embodiment of the present invention;
FIG. 4 is a perspective view of an embodiment of a microfluidic chip provided in an embodiment of the present invention;
FIG. 5 is a schematic diagram of a partial cross-sectional structure of an embodiment of a magnetic bead releasing system according to the present invention;
fig. 6 is a schematic partial cross-sectional view of another embodiment of a magnetic bead releasing system according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
According to the invention, the electromagnet is preset at the position of the storage part of the liquid magnetic bead labeled ligand of the microfluidic chip, the magnetic bead is adsorbed by the electromagnet, and when the magnetic bead releasing device is used, the magnetic bead is released, so that the magnetic bead can uniformly and completely flow out.
Example one
As shown in fig. 1, the embodiment of the present invention is achieved by providing a magnetic bead release mechanism, including:
a base 1 for placing the microfluidic chip 2;
an electromagnet 12;
the base 1 is provided with a placing groove 11 for placing the microfluidic chip 2, and the electromagnet 12 is arranged at a preset position below the placing groove 11.
According to the invention, the electromagnet 12 is used for collecting the magnetic beads in the liquid magnetic bead labeling ligand to a preset position, and when the device is used, the power supply of the electromagnet 12 is cut off, so that the electromagnet 12 loses magnetic force, and the magnetic beads are released, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead labeling ligand and are completely released; the detection accuracy can be effectively improved.
Example two
As shown in fig. 2 to 5, an embodiment of the present invention further provides a magnetic bead releasing system, where the magnetic bead releasing mechanism of the system and the microfluidic chip 2 disposed on the magnetic bead releasing mechanism are provided;
the microfluidic chip 2 is placed in the placing groove 11 of the base 1;
the electromagnet 12 collects the magnetic beads in the liquid magnetic bead labeling ligand to a preset position, the magnetic beads are released when the electromagnet is used, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead labeling ligand.
According to the invention, the electromagnet 12 is used for collecting the magnetic beads in the liquid magnetic bead labeling ligand to a preset position, and when the device is used, the power supply of the electromagnet 12 is cut off, so that the electromagnet 12 loses magnetic force, and the magnetic beads are released, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead labeling ligand and are completely released; the detection accuracy can be effectively improved.
EXAMPLE III
As shown in fig. 4 and 5, the microfluidic chip 2 includes:
a substrate 21;
labeling a ligand by liquid magnetic beads;
a storage part 22 disposed on the substrate 21 for storing a liquid magnetic bead labeled ligand;
the storage part 22 is provided with a breakable spacer 221 at the joint with the channel 23, the breakable spacer 221 is used for cutting off the communication between the channel 23 and the storage part 22, so that the storage part 22 becomes an independent sealed cavity, and when the channel 23 and the storage part 22 are needed, the breakable spacer is broken; and the depth of the channel 23 is not limited, and may be disposed on the top, middle or extending from the top of the substrate 21 to the bottom of the substrate 21, but cannot penetrate through the substrate 21.
As shown in fig. 6, in another embodiment, the trench 23 is disposed at the bottom of the substrate 21 and one side thereof penetrates through the substrate 21, the substrate 21 is provided with a thin film 211 at the side thereof through which the trench 23 penetrates, and the thin film 211 is made of the same material as the memory portion 22.
When the storage part 22 is pressed by external force, the breakable septum is broken, and the liquid magnetic bead labeled ligand flows out of the storage part 22.
Specifically, the storage portion 22 is a sealed cavity, and the sealing material is an elastic material or a high-barrier film, specifically, a plastic, a rubber, an aluminum foil or a high-barrier film, wherein the sealing material may be composed of the same material or a combination of multiple materials. The breakable separating sheet is a film or other materials which are easy to be locally broken under the extrusion action of external force, etc. Under physical pressure, the breakable septum of the reservoir 22 may be partially or completely broken, thereby releasing the stored liquid magnetic bead labeled ligand. The substrate is also provided with a sample addition part 24.
In another optional embodiment of this embodiment, the storage part 22 is specifically, as shown in fig. 4 and 5, the storage part 22 of the microfluidic chip 2 includes a cavity and a sac placed in the cavity, a part of weak points which are breakable are arranged at the lower part of the sac, the weak points form a breakable partition 221, a liquid magnetic bead labeled ligand is placed in the sac, the sac is fixed in the cavity by gluing, and a gap between the sac and the cavity is sealed to seal the whole storage part 22; part of the sac is exposed outside the cavity, so that the sac is pressed when in use, the breakable septum 221 is pressed by the liquid magnetic bead labeled ligand, and the liquid magnetic bead labeled ligand flows out of the storage part 22 after the breakable septum 221 is broken. Furthermore, the electromagnet 12 is preset at the breakable septum 221, and is configured to collect magnetic beads in the liquid magnetic bead labeled ligand at the breakable septum 221, and after the breakable septum is broken, the magnetic beads can flow out together with the liquid magnetic bead labeled ligand, so as to prevent the magnetic beads from precipitating.
Example four
As shown in fig. 5, the bottom of the storage part 22 of the microfluidic chip 2 is connected to the channel 23. When the breakable septum is broken, the liquid magnetic bead labeled ligand flows out through the channel 23, and the channel 23 is arranged at the bottom of the cavity 11, so that the liquid magnetic bead labeled ligand cannot be retained in the storage part 22, and the liquid magnetic bead labeled ligand can flow out conveniently.
EXAMPLE five
At least one of the channel 23 and the memory part 22 adopts a micro-channel structure, and at least one dimension of the micro-channel 23 is a micrometer scale. The micro-channel structure of the memory portion 22 means that the cavity of the memory portion 22 has a micro-channel structure.
The micro-fluidic chip 2 integrates and embeds all reagent components (sample liquid, enzyme-labeled ligand, magnetic bead labeled ligand, cleaning liquid, luminescent liquid and the like) required in the detection process into the micro-fluidic chip 2, realizes one-key operation of the micro-fluidic chip 2 (detection can be realized only by pressing a start key without complex operation) under the operation of a matched instrument through the ingenious micro-channel 23 design, and realizes whole blood separation, immunoreaction, cleaning separation and chemiluminescence detection, thereby avoiding the defects and defects of simple structural design, complex operation during detection and the like in the conventional micro-fluidic chip 2. And the defect that the traditional chemiluminescence apparatus can only detect serum or plasma but not a whole blood sample is overcome.
EXAMPLE six
The liquid magnetic bead labeled ligand is a liquid homogeneous phase magnetic label ligand, and the liquid homogeneous phase magnetic label ligand comprises magnetic beads, a temperature sensitive material and a solution. The magnetic beads comprise one or more of streptavidin magnetic beads, antibody modified magnetic beads and antigen modified magnetic beads; the temperature sensitive material is a thermoreversible gel comprising one or more of gelatin, agar, alginate, carrageenan, hydroxymethyl cellulose, acacia, guar gum, locust bean gum, pectin, starch, and xanthan gum; the solution is a buffer system containing surfactant and protein. Because the liquidity of the liquid is good, the liquid homogeneous phase magnetic labeling ligand, the enzyme labeling ligand and the added sample are fully mixed during detection, the reaction speed can be effectively improved, and the detection sensitivity, repeatability and accuracy are improved.
The enzyme-labeled ligand comprises an enzyme-or luminescent agent-labeled ligand.
The enzyme includes: one or more of horseradish peroxide and alkaline phosphatase; the luminescent agent includes: one or more of acridinium ester, ABEI, a fluorescent dye, a fluorescent protein or a fluorescent microsphere.
Specifically, the ligands include: one or more of an antigen, an antibody, a hapten and a nucleic acid.
In this embodiment, the enzyme-labeled ligand may be bound to the magnetic bead-labeled ligand (e.g., double antibody sandwich) or may compete with the magnetic bead-labeled ligand (e.g., competition). The ligand marked by the luminescent agent and the ligand marked by the enzyme can be the same as or different from the ligand marked by the magnetic beads. Preferably, in one embodiment of the invention, the analyte is detected in a double antibody sandwich method by selecting two different antibodies as the enzyme-labeled ligand and the magnetic bead-labeled ligand. In another embodiment of the present invention, an antigen and an antibody are selected as the enzyme-labeled ligand and the magnetic bead-labeled ligand, respectively, to detect the sample by a competitive method.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A magnetic bead release mechanism, the mechanism comprising:
a base for placing the microfluidic chip;
an electromagnet;
the base is provided with a placing groove for placing the microfluidic chip, and the electromagnet is arranged at a preset position below the placing groove.
2. A magnetic bead releasing system, comprising the magnetic bead releasing mechanism of claim 1 and a microfluidic chip;
the microfluidic chip is placed in the placing groove of the base;
the electromagnet collects the magnetic beads in the liquid magnetic bead marking ligand to a preset position, the magnetic beads are released when the electromagnet is used, and the magnetic beads uniformly flow out along with the solution in the liquid magnetic bead marking ligand.
3. The magnetic bead releasing system according to claim 2, wherein the microfluidic chip comprises:
a substrate;
labeling a ligand by liquid magnetic beads;
a storage part arranged on the substrate and used for storing liquid magnetic bead labeled ligands;
the storage part is provided with a channel arranged on the substrate, and a breakable spacer is arranged at the joint of the storage part and the channel;
and under the external force extrusion of the storage part, the breakable septum is broken, and the liquid magnetic bead labeled ligand flows out of the storage part.
4. The bead releasing system according to claim 3, wherein a bottom of the reservoir is connected to the channel.
5. The system for releasing magnetic beads according to claim 4, wherein at least one of the channel and the storage part adopts a microchannel structure, and at least one dimension of the microchannel is a micrometer scale.
6. The system for releasing magnetic beads of claim 3, wherein the liquid magnetic bead labeled ligand comprises magnetic beads, temperature sensitive material and solution.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921005003.9U CN210954058U (en) | 2019-06-27 | 2019-06-27 | Magnetic bead releasing mechanism and system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921005003.9U CN210954058U (en) | 2019-06-27 | 2019-06-27 | Magnetic bead releasing mechanism and system |
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| CN210954058U true CN210954058U (en) | 2020-07-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208527A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic bead relieving mechanism and system |
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- 2019-06-27 CN CN201921005003.9U patent/CN210954058U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110208527A (en) * | 2019-06-27 | 2019-09-06 | 深圳华迈兴微医疗科技有限公司 | A kind of magnetic bead relieving mechanism and system |
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