CN2577283Y - Protein chip - Google Patents
Protein chip Download PDFInfo
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- CN2577283Y CN2577283Y CN 02261367 CN02261367U CN2577283Y CN 2577283 Y CN2577283 Y CN 2577283Y CN 02261367 CN02261367 CN 02261367 CN 02261367 U CN02261367 U CN 02261367U CN 2577283 Y CN2577283 Y CN 2577283Y
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- base
- protein
- hole
- reaction chamber
- chip
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Abstract
The utility model discloses a protein chip, which comprises following components: a base seat (50), a reaction body (80), a reaction chamber (10), a patch (60) and a fixing clip (70), wherein, the base seat is provided with a hole array (52), the reaction body is arranged in a groove of the base seat, and the reaction chamber is provided with a hole array (12); a bulge (62) which is corresponding to the size and the shape of a hole arranged on the base seat (50) is arranged on the patch. Each component is separated with each other, when each component is overlapped together, the hole arrays are corresponding to each other.
Description
Technical field
The utility model relates to chip technology and external clinical diagnosis detection technique field, or rather, relates to a kind of protein-chip that is suitable for many indexs, the parallel detection of many person-portions.
Background technology
Utilizing antigen, antibody response to detect disease is a kind of maturation, important diagnose method, has obtained widespread use clinically.But, present immunological detection method, as: ELISA, emitting isotope mark, golden mark, fluorescence, chemiluminescence, time-resolved fluorescence etc., be that single index detects mostly, strictly speaking, be that a kind of single index, many person-portions detect, if can become index more than, the detection of many person-portions, then detection efficiency can improve a lot, and consumed time, manpower will significantly reduce.
The development of protein biochip technology, for rapid, parallelization, the high pass that realizes clinical diagnosis quantizes, promptly many indexs, many person-portions detect simultaneously that provide may.How Ruo-pan Huang, Thomas.o.Joos be to make up the protein microarray and carry out medical diagnosis on disease and study on films such as NC, PDVF, its method, principle are: put the antigen of corresponding disease or antibody on the film with high speed robot's point sample instrument (1), (2) adding test serum reacts on film, the reactant liquor of (2) labelling, (3) add chemical luminescence for liquid, (4) obtain image with CCD, (5) are analyzed image, diagnose the illness.Can make up a plurality of protein array on same the film, each dot matrix detects a person-portion, and the antigen or the antibody of various disease can be placed in each dot matrix inside, carries out many indexs and detects, and in view of the above, a film can be realized many person-portions, many indexs parallelization processing.
Yet, utilize above-mentioned principle to produce the convenient clinically protein-chip product of using, also must solve following problem: 1, will be separately each protein array, the immune response of different person-portions is carried out separately, 2, the two sides of film all will conveniently be cleaned, 3, prevent reagent seepage in the course of reaction (permeability of film is very big), 4, reacted film can conveniently take out, so that CCD carries out Flame Image Process.
Therefore, this area presses for the new protein-chip easy to use of exploitation.
Summary of the invention
The purpose of this utility model is at the problems referred to above, and a kind of many indexs, many person-portions parallelization disease detection protein-chip that can be used for clinical practice is provided.This chip not only can conveniently clean, and can prevent reagent seepage in the course of reaction, more can be reacted with the convenient taking-up of film, so that CCD carries out Flame Image Process.
The utility model provides a kind of protein-chip, and it contains following assembly:
One base, described base has the hole array, plate top fluted, the spacer region in hole slightly protrudes from the groove baseplane on the groove;
One reaction body, described reaction body is positioned in the groove of base, and described reaction body contains the proteins react diaphragm;
One reaction chamber, described reaction chamber has the hole array, the plane of oriented lower convexity, the plane of projection embeds the groove of base, and the reaction body is fixed in the groove of base;
One patch, have on the described patch with base on the corresponding projection of hole size, shape, projection stretches in the hole of base;
One geometrical clamp, described geometrical clamp have groove on it, are used for the edge of reaction chamber, reaction body and base that clamping stacks togather;
Wherein, above-mentioned each assembly can be separated from each other, and when repeatedly putting together, the hole array on reaction chamber 10 and the base is corresponding mutually.
In a preference of the present utility model, described reaction body also comprises:
One is positioned at the Upper gasket of diaphragm top, the porose array of described Upper gasket;
One is positioned at the lower gasket of diaphragm below, the porose array of described lower gasket;
When repeatedly putting together, the hole array of last lower gasket is corresponding mutually with the hole array of reaction chamber and base.
In another preference of the present utility model, the lower surface of Upper gasket and the upper surface of lower gasket are tacky surfaces.
In another preference of the present utility model, a plurality of protein square formations are arranged on the diaphragm, and when repeatedly putting together, the protein square formation on the diaphragm is corresponding mutually with the hole array of reaction chamber and base.
In another preference of the present utility model, described diaphragm for the unit average mark is slit into some Small diaphragm-pieces, has the slit with the protein array between the Small diaphragm-piece.
In another preference of the present utility model, described reaction chamber is to the shape of the plane of lower convexity, reaction body and base groove, big or small identical, corresponding, and the quantity in hole on it, size, position are identical, corresponding.
In another preference of the present utility model, described hole array has 4-10000 hole.
In another preference of the present utility model, described hole is rectangle, circle or oval.
In another preference of the present utility model, the profile of described reaction chamber, reaction body, base and patch is essentially rectangle, hexagon, octagon or circle.
In another preference of the present utility model, the quantity of described geometrical clamp is 2-6.
Description of drawings
Fig. 1 is the exploded view of a kind of example of the present utility model.
Fig. 2 is the skeleton view of the protein-chip shown in Figure 1 that fits together.
Embodiment
Referring to Fig. 1 and Fig. 2, protein-chip of the present utility model comprises reaction chamber 10, reaction body 80, base 50, geometrical clamp 70 and 60 5 assemblies of patch, and each assembly is separate.
Described base 50 has hole array 52, plate top fluted, and the spacer region 56 in hole slightly protrudes from the groove baseplane on the groove.
Described reaction body 80 is positioned in the groove of base, and described reaction body contains proteins react diaphragm 30.
Described reaction chamber 10 has hole array 12, the plane of oriented lower convexity, and the plane of projection embeds the groove of base 50, and reaction body 80 is fixed in the groove of base.Porose spacer region 16 between each hole of hole array 12.Preferably, span district 16 is raised in the upper surface of reaction chamber.
Have on the described patch 60 with base 50 on hole size, shape corresponding protruding 62, projection 62 stretches in base 50 holes, thereby prevents the seepage of liquid.
Described geometrical clamp 70 has groove on it, is used for the edge of reaction chamber 10, reaction body 80 and base 50 that clamping stacks togather.
Wherein, above-mentioned each assembly can be separated from each other, and when repeatedly putting together, the hole array on reaction chamber 10 and the base 50 is corresponding mutually.
As for the material of reaction chamber 10, reaction body 80, base 50 and patch 60, can select plastics, glass, metal or stupalith for use.
The material of geometrical clamp 70 can be selected plastics, metal material for use.
Protein diaphragm as reaction body main member can be various diaphragms commonly used in the protein-chip field, for example cellulose nitrate (NC) diaphragm, PDVF diaphragm etc.
In each hole of the hole of base of the present utility model array, can carry out the immunological response of different person-portions, plate top fluted, the spacer region of groove endoporus protrudes from the baseplane of groove, the reaction body is positioned in the groove of base, reaction chamber has the plane of projection downwards, the plane of projection embeds the groove of base, to react body is fixed in the groove, patch is positioned at the below of base, have on it with base on the identical projection of hole size, shape, projection stretches in the base aperture, prevent the reactant liquor seepage, can conveniently take out again during flushing.Geometrical clamp has groove shape structure, and the edge of reaction chamber, base and patch can snap in wherein, and reaction chamber, reaction body, base and patch are fixed together.
In another preference of the present utility model, described reaction body 80 comprises: Upper gasket 20, diaphragm 30 and lower gasket 20.Upper gasket 20 has hole array 22 and span district 26.Lower gasket 40 has hole array 42 and span district 46.The upper surface toughness of the lower surface of Upper gasket and lower gasket has a plurality of protein square formations on the diaphragm, the adhesive faces of two pads is relative, and in the middle of diaphragm was sandwiched in, the protein square formation on the diaphragm was corresponding with the hole array on the pad.
In another preference of the present utility model, described diaphragm is that unit is divided into a plurality of Small diaphragm-pieces with the protein microarray, the slit is arranged between the Small diaphragm-piece, each Small diaphragm-piece and top square formation correspondence reaction chamber, the hole above the base, reaction between the different holes of reaction chamber is carried out separately, and reagent solution can not interpenetrate.
Reaction chamber described in the utility model is identical, corresponding with shape, the size of base upper groove to the plane of lower convexity, pad, and the quantity in hole on it, size, position are identical, corresponding.
Size as for each hole of the hole array of assemblies such as reaction chamber and base is not particularly limited, and for example is about 1 millimeter * 1 millimeter to 20 millimeters * 20 millimeters, preferably is about 5-10 millimeter * 5-10 millimeter.
As for hole depth, also be not particularly limited, be generally the 1-20 millimeter, preferably be the 5-10 millimeter.
The volume of each reacting hole also is not particularly limited, and is generally 25 microlitres-5000 microlitre, preferably is about 100-500 microlitre.
Major advantage of the present utility model is:
A is simple in structure.
B assembling and convenient disassembly can prevent the cross pollution of sample effectively, are easy to realize the robotization that detects.
C once can detect many people, and everyone can detect a plurality of indexs, has shortened detection time, has saved manpower, has improved the efficient of medical diagnosis on disease greatly.
Be that instantiation is further set forth the utility model with the virus hepatitis detection chip below, but do not limit scope of the present utility model.
Embodiment 1
Adopt Fig. 1, protein-chip shown in Figure 2.A kind of protein-chip that the utility model provides comprises reaction chamber 10, reaction body 80, base 50, geometrical clamp 70 and patch 60 5 parts, each assembly is separate, the reaction chamber 10 of wherein said protein-chip is porose plastic plate, the plane that downward protrusion is arranged on the plate, the employed hole of the utility model is a square, and the quantity in hole is 48.The reaction body 80 form by 2 pads 40 and 1 diaphragm 30, pad 40 is an elastomeric material, the single face toughness, on 48 holes are arranged; Diaphragm 30 is the NC film, on 48 protein microarrays are arranged, each microarray is made up of 5 * 5 points, and the antigen or the antibody of 12 kinds of marks of first, second, third, fourth, penta 5 kinds of hepatitis is arranged on it, each protein microarray is corresponding with the hole on the reaction chamber 10.Two pad 40 adhesive faces are relative, in the middle of diaphragm 30 is sandwiched in, to prevent sample seepage, cross pollution; Base 50 also is 48 hole plastic plates, above fluted, the size of groove, shape are identical with the protrusion plane and the reaction body 80 of reaction chamber 10, the spacer region of groove endoporus protrudes from the baseplane of groove; Reaction body 80 is positioned in the groove of base 50, and the plane of protruding below with reaction chamber 10 on it joins; Geometrical clamp 70 is a plastic material, rectangular structure, and interior angle one side has groove, is stuck in reaction chamber 10, base 50 four jiaos, will be connected as a single entity except that the entire chip the patch 60; 48 projectioies identical with size, the shape in base 50 holes are arranged on the patch 60, extend in the hole of base 50, prevent the reactant liquor seepage.
Number of assembling steps
(1) projection on the patch is corresponding with the hole on the base, and patch, base are connected as a single entity.
(2) diaphragm is affixed on the adhesive faces of one of them pad, cut along the centre of hole wall, and be that unit is divided into 48 blockages with the protein array with diaphragm, again second pad is affixed on the diaphragm.
(3) the reaction body has facing up of protein array, puts into the groove of base.
(4) reaction chamber snaps in the groove of base on the plane of protrusion downwards,
(5) 4 geometrical clamps are stuck in reaction chamber, reaction body, base union body four jiaos.Keep the position in the hole on reaction chamber, reaction body and the base corresponding in the assembling process.
Using method
(1) the blood serum sample 100ul to be checked that adds certain dilution (does blank well, negative control hole and positive control hole) simultaneously in the reacting hole of reaction chamber, hatches 30 minutes for 37 ℃.
(2) take off patch, chip two sides washing 4 times pats dry, and loads onto patch.
(3) add reactant liquor 100ul in each reacting hole, 37 ℃ of temperature were bathed 30 minutes.
(4) take off patch, chip two sides washing 4 times pats dry.
(5) add luminescent solution 50ul.
(6) take off geometrical clamp, reaction chamber, chip is put into the CCD chip detector take pictures.
(7) computer image analysis, the bright spot on the image, expression positive reaction.
Should be understood that in addition those skilled in the art can make various changes or modifications the utility model after having read above-mentioned teachings of the present utility model, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. a protein-chip is characterized in that, it contains following assembly:
One base (50), described base (50) has hole array (52), plate top fluted, the spacer region in hole (56) slightly protrudes from the groove baseplane on the groove;
One reaction body (80), described reaction body (80) is positioned in the groove of base, and described reaction body contains proteins react diaphragm (30);
One reaction chamber (10), described reaction chamber (10) has hole array (12), the plane of oriented lower convexity, the groove that the plane of projection embeds base (50) will react body (80) and be fixed in the groove of base;
One patch (60), have on the described patch (60) with base (50) on the corresponding projection of hole size, shape (62), the projection (62) stretch in the hole of base (50);
One geometrical clamp (70), described geometrical clamp (70) have groove on it, are used for the edge of reaction chamber (10), reaction body (80) and base (50) that clamping stacks togather;
Wherein, above-mentioned each assembly can be separated from each other, and when repeatedly putting together, the hole array on reaction chamber (10) and the base (50) is corresponding mutually.
2. protein-chip according to claim 1 is characterized in that, described reaction body (80) also comprises:
One is positioned at the Upper gasket (20) of diaphragm (30) top, the porose array of described Upper gasket (22);
One is positioned at the lower gasket (40) of diaphragm (30) below, the porose array of described lower gasket (42);
When repeatedly putting together, the hole array of last lower gasket is corresponding mutually with the hole array of reaction chamber (10) and base (50).
3. protein-chip as claimed in claim 2 is characterized in that, the lower surface of Upper gasket and the upper surface of lower gasket are tacky surfaces.
4. protein-chip as claimed in claim 1 is characterized in that, a plurality of protein square formations are arranged on the diaphragm (30), and when repeatedly putting together, the protein square formation on the diaphragm (30) is corresponding mutually with the hole array of reaction chamber (10) and base (50).
5. protein-chip according to claim 2 is characterized in that, described diaphragm (30) for the unit average mark is slit into some Small diaphragm-pieces, has the slit with the protein array between the Small diaphragm-piece.
6. protein-chip according to claim 1 is characterized in that, described reaction chamber (10) is to the shape of the plane of lower convexity, reaction body (80) and base (50) groove, big or small identical, corresponding, and the quantity in hole on it, size, position are identical, corresponding.
7. protein-chip as claimed in claim 1 is characterized in that, described hole array has 4-10000 hole.
8. protein-chip as claimed in claim 1 is characterized in that, described hole is rectangle, circle or oval.
9. protein-chip as claimed in claim 1 is characterized in that, the profile of described reaction chamber, reaction body, base and patch is essentially rectangle, hexagon, octagon or circle.
10. protein-chip as claimed in claim 1 is characterized in that, the quantity of described geometrical clamp is 2-6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02261367 CN2577283Y (en) | 2002-11-08 | 2002-11-08 | Protein chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02261367 CN2577283Y (en) | 2002-11-08 | 2002-11-08 | Protein chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2577283Y true CN2577283Y (en) | 2003-10-01 |
Family
ID=33728174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02261367 Expired - Fee Related CN2577283Y (en) | 2002-11-08 | 2002-11-08 | Protein chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2577283Y (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325148C (en) * | 2005-07-04 | 2007-07-11 | 天津工业大学 | Detecting assemble for film products |
| CN102279260A (en) * | 2010-06-12 | 2011-12-14 | 北京众合百克科学仪器技术有限公司 | Clamping device for clamping ELISA (Enzyme-Linked Immuno Sorbent Assay) plate in chemoluminescence immunoassay instrument |
| CN102539740A (en) * | 2010-12-29 | 2012-07-04 | 河北省健海生物芯片技术有限责任公司 | Protein chip and method for detecting 80 kinds of autoantibodies simultaneously |
| CN102707044A (en) * | 2012-05-31 | 2012-10-03 | 李云峰 | Vertical western blot antibody incubation box |
| CN103091488A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay |
| CN103091483A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Production method of membrane diagnostic products |
| CN104884605A (en) * | 2012-08-24 | 2015-09-02 | 耶鲁大学 | System, device and method for high-throughput multi-plexed detection |
-
2002
- 2002-11-08 CN CN 02261367 patent/CN2577283Y/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1325148C (en) * | 2005-07-04 | 2007-07-11 | 天津工业大学 | Detecting assemble for film products |
| CN102279260A (en) * | 2010-06-12 | 2011-12-14 | 北京众合百克科学仪器技术有限公司 | Clamping device for clamping ELISA (Enzyme-Linked Immuno Sorbent Assay) plate in chemoluminescence immunoassay instrument |
| CN102539740A (en) * | 2010-12-29 | 2012-07-04 | 河北省健海生物芯片技术有限责任公司 | Protein chip and method for detecting 80 kinds of autoantibodies simultaneously |
| CN102707044A (en) * | 2012-05-31 | 2012-10-03 | 李云峰 | Vertical western blot antibody incubation box |
| CN104884605A (en) * | 2012-08-24 | 2015-09-02 | 耶鲁大学 | System, device and method for high-throughput multi-plexed detection |
| CN104884605B (en) * | 2012-08-24 | 2018-05-18 | 耶鲁大学 | For the systems, devices and methods of high-throughput multi detection |
| CN103091488A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Sample application plate for holding membrane strips to be coated in membrane diagnosis immunoassay |
| CN103091483A (en) * | 2013-01-11 | 2013-05-08 | 苏州浩欧博生物医药有限公司 | Production method of membrane diagnostic products |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20031001 Termination date: 20091208 |