CN211401881U

CN211401881U - Batch slide staining rack

Info

Publication number: CN211401881U
Application number: CN202020051921.1U
Authority: CN
Inventors: 秦岩辉; 蔡田; 徐尚; 孙贵宾; 张硕; 任存邦; 魏娟; 孙武举; 杨红云; 王则宇
Original assignee: Autobio Diagnostics Co Ltd
Current assignee: Autobio Diagnostics Co Ltd
Priority date: 2020-01-10
Filing date: 2020-01-10
Publication date: 2020-09-01
Anticipated expiration: 2030-01-10

Abstract

The utility model discloses a batch slide dyeing rack, which comprises a rack body and a drop dyeing box which is obliquely supported and fixed on the left and right side walls of the rack body through a support rod; a liquid storage cavity is arranged at the upper part of the inner cavity of the drop dyeing box, the top of the liquid storage cavity is communicated with a liquid injection port, a plurality of liquid outlets positioned above the inner cavity of the frame body are uniformly arranged on the bottom wall of the drop dyeing box at intervals along the length direction, and each liquid outlet is communicated with the bottom of the liquid storage cavity through a drop liquid runner; the middle part of the inner cavity of the frame body is transversely provided with a supporting plate, the left end of the supporting plate is hinged to the inner side surface of the left side wall of the frame body through a horizontal hinge shaft, the right end of the supporting plate is provided with a handheld part which penetrates out of a strip-shaped longitudinal slot hole formed in the right side wall of the frame body, an upper opening slide glass carrying box positioned right below the drip dyeing box is movably clamped on the supporting plate, and a plurality of annular structure slide glass clamping edges which are in one-to-one adaptive correspondence with the liquid outlets are distributed on the inner bottom wall of the slide. The utility model has the advantages of simple structure, easy operation and low processing cost.

Description

Batch slide staining rack

Technical Field

The utility model belongs to the technical field of microscope dyeing microscopic examination technique and specifically relates to a batch slide staining rack is related to.

Background

Microscopic staining microscopy is a basic means for clinical detection of microbial pathogens, and common staining methods include gram staining, ink staining, acid-fast staining and the like. At present, most laboratories adopt manual staining, and the hand or tweezers are used for clamping slides for staining; the manual dyeing consumes long time, has low efficiency, and liquid is easy to splash and is stained with gloves, clothes and the like. Some full-automatic dyeing machines on sale on the market have relatively high cost and insufficient market promotion although the dyeing efficiency is greatly improved compared with manual operation, and are not suitable for some small and medium-sized laboratories. In addition, some batch dyeing frames are available for meeting the batch processing requirements, but most batch dyeing is carried out in a dip dyeing mode, the dip dyeing can cause waste of dye liquor, and if the dye liquor is reused, the dye liquor can cause pollution.

Disclosure of Invention

The utility model aims to provide a batch slide dyeing rack with simple structure and simple operation.

In order to achieve the above purpose, the utility model can adopt the following technical proposal:

the batch slide dyeing rack of the utility model comprises a rack body formed by surrounding a front side wall, a rear side wall, a left side wall and a right side wall, and a drop dyeing box which is obliquely supported and fixed on the left side wall and the right side wall of the rack body through a support rod; a liquid storage cavity is arranged at the upper part of the inner cavity of the dye dripping box, the top of the liquid storage cavity is communicated with a liquid injection port extending out of the dye dripping box, a plurality of liquid outlets positioned above the inner cavity of the frame body are uniformly arranged on the bottom wall of the dye dripping box at intervals along the length direction, and each liquid outlet is communicated with the bottom of the liquid storage cavity through a liquid dripping flow channel; the middle part of the inner cavity of the frame body is transversely provided with a supporting plate, the left end of the supporting plate is hinged to the inner side surface of the left side wall of the frame body through a horizontal hinge shaft, the right end of the supporting plate is provided with a handheld part which penetrates out of a strip-shaped longitudinal slot hole formed in the right side wall of the frame body, an upper opening slide box positioned right below the drip dyeing box is movably clamped on the supporting plate, and a plurality of annular-structure slide clamping edges which are in one-to-one adaptive correspondence with the liquid outlets and used for clamping and fixing slides to be drip dyed are distributed on the inner bottom wall of the slide box.

The included angle alpha between the drop dyeing box and the horizontal plane is 45-75 degrees.

The liquid dropping flow passage at the liquid outlet is of a horn-shaped structure with a small upper part and a big lower part, and a screen plate or a pore plate is arranged at the liquid outlet.

The dropping liquid runner include with the sprue that the stock solution chamber is linked together, the lower extreme intercommunication of sprue has two one-level branch runners that extend to both sides, each the lower extreme of one-level branch runner all communicates has two second grade branch runners that extend to both sides, each the lower extreme of two-level branch runner all corresponds a intercommunication liquid outlet.

Each the dropping liquid runner on the liquid outlet is the independent type of bending runner, the length homoenergetic of the type of bending runner equals to ensure that the liquid in each type of bending runner can flow out simultaneously.

A plurality of bayonets are uniformly arranged on the long edges of the two sides of the longitudinal slotted hole at intervals along the length direction, and a pair of elastic clamping plates which are matched and clamped in the bayonets are arranged on the hand-held part.

The cover plate is hinged to the opening of the slide glass box, a plurality of through holes which are in one-to-one adaptive correspondence with the liquid outlets are formed in the cover plate, the aperture of each through hole is larger than the diameter of each liquid outlet, and therefore falling dye liquid can penetrate through the through holes and drip onto to-be-dripped glass slides placed in the slide glass clamping edges.

And drip-dyeing region detection holes which are in one-to-one adaptation correspondence with the through holes are formed in the bottom wall of the slide glass box on the inner side of the slide glass clamping edge, the aperture of each drip-dyeing region detection hole is consistent with that of each through hole, and liquid leakage holes are uniformly distributed in the bottom wall of the slide glass box between each drip-dyeing region detection hole and the slide glass clamping edge.

The glass slide box is clamped and fixed on the supporting plate through a pair of door-shaped clamping strips movably inserted and fixed on the supporting plate, the two door-shaped clamping strips are positioned on two sides of the through hole, and an arc-shaped groove matched and attached with the door-shaped clamping strips is formed in the top surface of the glass slide box.

And supporting legs are longitudinally arranged at the four corners of the bottom of the frame body.

The utility model has the advantages of simple structure, easy operation and low processing cost. By using the idea of fluid control, the design of the liquid dropping flow channel is utilized to synchronously distribute the dye solution, so that a batch of slide glasses to be subjected to drop dyeing and placed in the slide glass box are dyed, and the aim of batch dyeing is fulfilled; in addition, the design of the slide glass box is more convenient for smear and positioning dyeing, so that the smear position is unified, the purpose of protecting the slide glass is achieved, the slide glass is convenient to mount, transfer and move, meanwhile, the later stage microscopic examination is facilitated, and the light path and focusing are not influenced.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a right side view of fig. 1.

Fig. 3 is an enlarged sectional view taken along line a-a of fig. 2.

Fig. 4 is a schematic view of another version of the drip flow path of fig. 3.

Figure 5 is a top view of the pallet of figure 1 (with a slide cassette clamped thereto).

Figure 6 is a top view of the slide cassette of figure 1 (with the cover panel open).

Figure 7 is a right side view of the slide cassette of figure 1.

Detailed Description

As shown in figure 1, 2, batch slide dyeing frame, include and surround support body 1 that forms by preceding, back, left and right lateral wall to and prop up through branch 2 slope admittedly in support body 1 left and right lateral wall on drip and dye box 3, drip and dye the contained angle alpha between box 3 and the horizontal plane and be 45~75, be provided with stock solution chamber 4 on the inner chamber upper portion that drips and dye box 3, the top intercommunication of stock solution chamber 4 has the liquid mouth 5 of annotating that extends to drip and dye box 3 outer, annotate liquid mouth 5 joinable syringe, funnel, notes liquid pump etc. to add the dye liquor in to it.

Evenly spaced apart along length direction on the diapire of drop dyeing box 3 and be equipped with a plurality of liquid outlets 6 that are located 1 inner chamber tops of support body, each liquid outlet 6 all is linked together bottom 4 with the stock solution chamber through the dropping liquid runner. Specifically, as shown in fig. 3, the dropping liquid flow channel includes a main flow channel 13 communicated with the liquid storage chamber 4, the lower end of the main flow channel 13 is communicated with two primary branch flow channels 14 extending to both sides, the lengths of the two primary branch flow channels 14 are equal, the lower end of each primary branch flow channel 14 is communicated with two secondary branch flow channels 15 extending to both sides, the lengths of each secondary branch flow channel 15 are equal, and the lower ends of the secondary branch flow channels are correspondingly communicated with the liquid outlet 6. As shown in fig. 4, the dropping liquid flow channel may also be an independent bent flow channel 16, and each liquid outlet 6 is communicated with one bent flow channel 16, only by ensuring that the lengths of the bent flow channels 16 are equal; of course, the bending times between the bending flow channels 16 are not easily different from each other, so as to ensure that the liquid in each bending flow channel 16 can flow out simultaneously, and the specific length of the bending flow channel 16 and the number of the bending flow channels can be calculated according to the flowing experiment of the liquid.

It should be noted that, when the drip flow channel adopts a fork-shaped structure as shown in fig. 3, the number of the liquid outlets 6 formed in the bottom wall of the drip dyeing box 3 is 4, and of course, 8 or 16 liquid outlets 6 can be formed, and only the flow channel needs to be graded downwards according to the number of the liquid outlets 6; however, when the dropping liquid flow passage is a bent flow passage 16 as shown in fig. 4, the number of the liquid outlets 6 may be set arbitrarily.

In addition, the dropping liquid flow channel at the liquid outlet 6 is of a horn-shaped structure with a small upper part and a big lower part, and a screen plate or a pore plate is arranged at the liquid outlet 6, so that the dye liquid flows down in a scattering shape, and the dye liquid smearing device is more favorable for smearing evenly.

The middle part of the inner cavity of the frame body 1 is transversely provided with a supporting plate 7, the left end of the supporting plate 7 is hinged to the inner side surface of the left side wall of the frame body 1 through a horizontal hinge shaft 8, the right end of the supporting plate 7 is provided with a handheld part 10 which penetrates out of a strip-shaped longitudinal slot hole 9 formed in the right side wall of the frame body 1, and the supporting plate 7 can easily rotate up and down in the longitudinal slot hole 9 through the handheld part 10. In addition, a plurality of bayonets 17 are uniformly arranged on the long sides of the two sides of the longitudinal slot 9 at intervals along the length direction, a pair of elastic clamping plates 18 which are adaptive to be clamped in the bayonets 17 are arranged on the handheld part 10, when the two elastic clamping plates 18 are pressed down, the handheld part 10 can move up and down along the longitudinal slot 9, and when the elastic clamping plates 18 are loosened, the supporting plate 7 can be clamped.

An upper opening glass slide box 11 positioned under the drip-dyeing box 3 is movably clamped on the supporting plate 7, and a plurality of annular-structure glass slide clamping edges 12 which are matched and corresponding to the liquid outlets 6 one by one and used for clamping the glass slide to be drip-dyed are distributed on the inner bottom wall of the glass slide box 11. In order to further protect the glass slides placed in the glass slide box 11, the opening of the glass slide box 11 is hinged with a cover plate 19, the cover plate 19 is provided with a plurality of through holes 20 which are matched and corresponding to the liquid outlets 6 one by one, and the aperture of each through hole 20 is slightly larger than the diameter (generally 4-6 mm) of each liquid outlet 6, so that the falling dye solution can pass through the through holes 20 and drip onto the glass slides to be dripped and placed in the glass slide clamping edges 12. In order to realize microscopic examination of the slide glass under the condition of not taking out the slide glass, drip-dyeing region detection holes 21 which are in one-to-one adaptive correspondence with the through holes 20 are formed in the bottom wall of the slide glass box 11 positioned on the inner side of the slide glass clamping edge 12, the aperture of the drip-dyeing region detection holes 21 is consistent with that of the through holes 20, and the drip-dyeing region detection holes 21 just can form a smear region which contrasts on the slide glass, so that the good passing of a light path of the smear region is realized, and the microscopic examination and the focusing during the microscopic examination are easily realized. In addition, leakage holes 22 are uniformly distributed on the bottom wall of the slide glass box 11 between the detection hole 21 of the drop dyeing area and the slide glass clamping edge 12, and the leakage holes 22 can easily realize the discharge of liquid during cleaning; meanwhile, the four corners of the bottom of the frame body 1 are longitudinally provided with supporting legs 25, so that the frame body 1 is lifted, and a tray for receiving leakage or cleaning liquid can be placed below the frame body 1.

In order to facilitate the installation of the slide glass box 11, a pair of door-shaped clamping strips 23 are movably inserted on the upper surface of the supporting plate 7, and the slide glass box 11 is easily clamped and fixed through the door-shaped clamping strips 23; in order to avoid influencing the falling of the dye liquor, two door-shaped clamping strips 23 are positioned at two sides of the row of through holes 20, and arc-shaped grooves 24 which are matched and attached with the door-shaped clamping strips 23 are formed in the top surface of the slide glass box 11.

During the use, operating personnel only need lay the slide glass in slide glass slide box 11, then lock apron 19, carry out the sample smear and dry through perforation 20 on it, dry the back and carry out the dyeing again. At the moment, the slide glass box 11 is clamped on the tray 7, dye liquor is injected into the liquid storage cavity 4 through the liquid port 5 by adopting a syringe, a funnel or an injection pump, the dye liquor enters the liquid dropping flow channel and flows out through the liquid outlet 6 at the lower end of the liquid dropping flow channel, and the dye liquor passes through the through hole 20 on the cover plate 19 and is dripped on the slide glass; at the moment, the hand-held part 10 is held by hand, the two elastic clamping plates 18 are pressed downwards, and the tray 7 is shaken up and down to enable the dye solution dropped on the glass slide to be uniformly coated; after dyeing is finished, the dyeing is carried out by washing with clear water, at the moment, the elastic sheet 18 on the hand-held part 10 is pressed down and clamped into the bayonet 17 at the lowest end of the longitudinal slotted hole 9, so that the tray 7 provided with the slide glass box 11 is inclined to drain water, and the whole dyeing process is finished. Taking gram stain as an example, the staining can be completed after a series of steps of initial staining, rinsing, mordant staining, rinsing, decolorizing, rinsing, airing, and after the whole staining operation is completed, the slide box 11 can be taken down for subsequent microscopic examination, and the device can be cleaned for the next use.

Claims

1. A batch slide staining rack, characterized in that: comprises a frame body (1) formed by encircling a front side wall, a rear side wall, a left side wall and a right side wall, and a drop dyeing box (3) obliquely supported and fixed on the left side wall and the right side wall of the frame body (1) through a support rod (2); a liquid storage cavity (4) is arranged at the upper part of the inner cavity of the drop dyeing box (3), the top of the liquid storage cavity (4) is communicated with a liquid injection port (5) extending out of the drop dyeing box (3), a plurality of liquid outlets (6) positioned above the inner cavity of the frame body (1) are uniformly arranged on the bottom wall of the drop dyeing box (3) at intervals along the length direction, and each liquid outlet (6) is communicated with the bottom of the liquid storage cavity (4) through a liquid dropping runner; the drip-dyeing slide rack is characterized in that a supporting plate (7) is transversely arranged in the middle of an inner cavity of the rack body (1), the left end of the supporting plate (7) is hinged to the inner side face of the left side wall of the rack body (1) through a horizontal hinge shaft (8), a handheld part (10) penetrating out of a strip-shaped longitudinal groove hole (9) formed in the right side wall of the rack body (1) is arranged at the right end of the supporting plate (7), an upper opening slide box (11) located right below the drip-dyeing box (3) is movably clamped on the supporting plate (7), and a plurality of annular-structure slide clamping edges (12) which are matched and corresponding to the liquid outlets (6) one by one and used for clamping slides to be drip-dyed are distributed on the inner bottom wall of the slide box.

2. The batch slide staining rack of claim 1, wherein: the included angle alpha between the drop dyeing box (3) and the horizontal plane is 45-75 degrees.

3. The batch slide staining rack of claim 1, wherein: the dropping liquid flow channel at the liquid outlet (6) is of a horn-shaped structure with a small upper part and a large lower part, and a screen plate or a pore plate is arranged at the liquid outlet (6).

4. The batch slide staining rack of claim 1, wherein: the dropping liquid runner include with sprue (13) that stock solution chamber (4) are linked together, the lower extreme intercommunication of sprue (13) has two one grades of branch runner (14) that extend to both sides, each the lower extreme of one grade of branch runner (14) all communicates two grades of branch runner (15) that extend to both sides, each the lower extreme of two grades of branch runner (15) all corresponds a intercommunication liquid outlet (6).

5. The batch slide staining rack of claim 1, wherein: each dropping liquid runner on liquid outlet (6) is independent type of bending runner (16), the length homoenergetic of type of bending runner (16) equals to ensure that the liquid in each type of bending runner (16) can flow out simultaneously.

6. The batch slide staining rack of claim 1, wherein: a plurality of bayonets (17) are arranged on the long edges of the two sides of the longitudinal slotted hole (9) at equal intervals along the length direction, and a pair of elastic clamping plates (18) which are adaptive to be clamped in the bayonets (17) are arranged on the handheld part (10).

7. The batch slide staining rack of claim 1, wherein: the opening of slide glass slide box (11) is articulated to have apron (19), seted up on apron (19) with a plurality of perforation (20) that liquid outlet (6) one-to-one adaptation corresponds, the aperture of perforation (20) is greater than the diameter of liquid outlet (6), ensures that the dye liquor of whereabouts can pass perforation (20) and drip to lay on waiting to drip to dye the slide glass in slide card limit (12).

8. The batch slide staining rack of claim 7, wherein: and drip-dyeing region detection holes (21) which are in one-to-one adaptive correspondence with the through holes (20) are formed in the bottom wall of the slide glass carrying box (11) on the inner side of the slide glass clamping edge (12), the aperture of each drip-dyeing region detection hole (21) is consistent with that of each through hole (20), and liquid leakage holes (22) are uniformly distributed in the bottom wall of the slide glass carrying box (11) between each drip-dyeing region detection hole (21) and the slide glass clamping edge (12).

9. The batch slide staining rack of claim 7, wherein: the glass slide box (11) is clamped on a pair of door-shaped clamping strips (23) which are movably inserted and arranged on the upper surface of the supporting plate (7), the two door-shaped clamping strips (23) are positioned on two sides of the through hole (20), and an arc-shaped groove (24) which is matched and attached with the door-shaped clamping strips (23) is formed in the top surface of the glass slide box (11).

10. The batch slide staining rack of claim 1, wherein: the bottom four corners of the frame body (1) are longitudinally provided with supporting legs (25).