CN211199245U

CN211199245U - Cell constant temperature culture apparatus

Info

Publication number: CN211199245U
Application number: CN201922215593.4U
Authority: CN
Inventors: 白玉
Original assignee: Xingtai Medical College
Current assignee: Xingtai Medical College
Priority date: 2019-12-12
Filing date: 2019-12-12
Publication date: 2020-08-07
Anticipated expiration: 2029-12-12

Abstract

The utility model discloses a cell constant temperature culture device, which comprises an incubator, a movable plate and a water tank, wherein the bottoms of the inner walls at the two sides of the incubator are respectively connected with the two sides of the movable plate in a sliding way through a square chute, the top end of the movable plate is respectively connected with the top parts of eight culture dishes in a clamping way, the middle part of the top end of the incubator is rotatably connected with the top part of a rotating rod, the top end of the rotating rod is fixedly connected with the middle part of the bottom end of a rotating disk, the bottom end of the rotating rod is fixedly connected with the top end of an electron microscope, and the position of the electron microscope and the position of the culture dishes are arranged in a one-to-one correspondence way, the cell constant temperature culture device can rotate the rotating disk if the specific condition of cells needs to be observed in the cell culture process, the rotating disk drives the electron microscope to, the cell is taken without opening the incubator in the observation process, and the cell is prevented from being polluted.

Description

Cell constant temperature culture apparatus

Technical Field

The utility model relates to a constant temperature culture apparatus, in particular to cell constant temperature culture apparatus.

Background

Cells (English name: cell) are not uniformly defined, and the more common method is: cells are the basic structural and functional units of an organism. It is known that all organisms except viruses are composed of cells, but viral life activities must be expressed in cells.

Generally, most microorganisms such as bacteria and protozoa are composed of one cell, i.e., unicellular organisms, and higher plants and higher animals are multicellular organisms. The cells can be divided into two types of prokaryotic cells and eukaryotic cells, but the division into three types is also proposed, namely ancient nuclear cells originally belonging to the prokaryotic cells are independently taken as the parallel type. The discipline of studying cells is called cell biology.

The cell body is tiny and can be observed under a microscope, and the shape is various. Mainly composed of nucleus and cytoplasm, and has cell membrane on the surface. Higher plants have cell walls outside the cell membrane, plastids in the cytoplasm, chloroplasts and vacuoles in the body, and mitochondria. Animal cells have no cell wall, usually centrosomes in the cytoplasm, and higher plant cells. The cells have the functions of movement, nutrition, reproduction and the like.

At present, most of cell constant-temperature culture devices directly utilize a heating plate and a cooling plate to carry out temperature rise and fall operation, and the temperature change of the temperature rise and fall operation is unstable, so that cell death can be caused, and the practicability of the cell constant-temperature culture devices is reduced; at present, cells in culture need to be taken out from a constant-temperature culture device for observation in the constant-temperature culture process, and cell pollution can be caused in the taking process.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell constant temperature culture apparatus to solve the current cell constant temperature culture apparatus temperature variation unstable problem that proposes in the above-mentioned background art.

to achieve the above object, the utility model provides a cell constant temperature culture device, including incubator, fly leaf and water tank, the bottom of incubator both sides inner wall is respectively through the both sides sliding connection of square spout with the fly leaf, the top of fly leaf is connected with the top block of eight culture dishes respectively, the middle part on incubator top is rotated with the top of dwang and is connected, the top of dwang and the middle part fixed connection of carousel bottom, the bottom of dwang and electron microscope's top fixed connection, and electron microscope's position and the position one-to-one setting of culture dish, the fixed temperature sensor that is equipped with in top of incubator one side inner wall, the fixed P L C controller that is equipped with in top of incubator one side, the bottom mounting of incubator is equipped with the water tank, the fixed heat pump that is equipped with in one side of the inside bottom of water tank, the output of heat pump and the fixed intercommunication of one end of transmission pipeline, the fixed cooling pump that is equipped with of the opposite side of the inside bottom of water tank, the output and the fixed intercommunication of cooling transmission pipeline's one end, the middle part of heating transmission pipeline and the middle part of cooling transmission pipeline are connected with the inside winding of incubator respectively, and the other end of heating transmission pipeline, the positive connection of transmission pipeline, the other end of transmission pipeline that is connected with the other end of transmission pipeline that the heating, the transmission pipeline that.

As an optimal technical scheme of the utility model, one side on incubator top is fixed and is equipped with one-way admission valve, the fixed one-way air outlet valve that is equipped with of opposite side on incubator top.

As an optimized technical scheme of the utility model, one side on carousel top is fixed and is equipped with the handle.

As an optimal technical scheme of the utility model, four corners of water tank bottom are all fixed and are equipped with the non slipping block.

As an optimized technical scheme of the utility model, the positive middle part of chamber door is fixed and is equipped with observation window.

As an optimized technical scheme of the utility model, the fixed metal strip that is equipped with in positive one side of chamber door, the fixed electro-magnet that is equipped with in positive one side of incubator, adsorb between electro-magnet and the metal strip and be connected.

as an optimal technical scheme of the utility model, the fixed electro-magnet switch, heat pump switch and the cooling pump switch that is equipped with in middle part of incubator one side, electro-magnet, heat pump and cooling pump are respectively through electro-magnet switch, heat pump switch and cooling pump switch and external power supply electric connection, temperature sensor, heat pump and cooling pump all with P L C controller electric connection.

compared with the prior art, the beneficial effects of the utility model are that, the utility model relates to a cell constant temperature culture apparatus, this culture apparatus monitors the temperature in the incubator through temperature sensor, heat or cooling operation through P L C controller control heat pump and cooling pump again, heat pump and cooling pump are with hot water or cold water through heating transmission pipeline and cooling transmission pipeline to incubator inside evenly heating or cooling, this kind of temperature rise and fall operation temperature variation is stable, can prevent that the cell from dying because of temperature variation is unstable, and then can effectively improve this culture apparatus's practicality, in the cell culture process, if need observe the cell particular case, rotatable carousel, the carousel drives electron microscope through the dwang and rotates, when electron microscope's camera lens is relative with the culture dish, the particular case accessible connecting wire of cell shows on the display screen, this observation process need not to open the incubator and takes the cell, and then prevent that the cell from being polluted.

Drawings

FIG. 1 is a schematic front view of the present invention;

Fig. 2 is a schematic structural view of the electron microscope of the present invention;

Fig. 3 is a schematic structural view of the movable plate of the present invention;

FIG. 4 is a schematic structural view of the heating and transporting pipeline of the present invention;

Fig. 5 is a schematic structural diagram of the water tank of the present invention.

in the figure, the device comprises a culture box 1, a movable plate 2, a temperature sensor 3, a one-way air inlet valve 4, a one-way air outlet valve 5, a turntable 6, an electron microscope 7, a PLC controller 8, a water tank 9, a heating transmission pipeline 10, a cooling transmission pipeline 11, a box door 12, an observation window 13, a metal strip 14, an electromagnet 15, a rotating rod 16, a culture dish 17, a heating pump 18, a cooling pump 19 and a cooling pump.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

please refer to fig. 1-5, the utility model provides a cell constant temperature culture apparatus, including incubator 1, movable plate 2 and water tank 9, the bottom of incubator 1 both sides inner wall is respectively through the both sides sliding connection of square spout with movable plate 2, the top of movable plate 2 is connected with the top block of eight culture dishes 17 respectively, the middle part on incubator 1 top is rotated with the top of dwang 16 and is connected, the top of dwang 16 and the middle part fixed connection of carousel 6 bottom, the bottom of dwang 16 and the top fixed connection of electron microscope 7, and the position of electron microscope 7 and the position one-to-one setting of culture dish 17, the top of incubator 1 one side inner wall is fixed and is equipped with temperature sensor 3, the top of incubator 1 one side is fixed and is equipped with P L C controller 8, the bottom mounting of incubator 1 is equipped with water tank 9, one side of the inside bottom of water tank 9 is fixed and is equipped with heat pump 18, the output of heat pump 18 and the fixed intercommunication of one end of heat transmission pipeline 10, the opposite side of the inside bottom of water tank 9 is fixed and is equipped with cooling pump 19, the output of cooling pump 19 and the fixed intercommunication of cooling transmission pipeline 11, the other end of the other side of heat transmission pipeline 11 and the other side of the coil of the other side of the incubator door of transmission pipeline 11 and the other end of the coil of transmission pipeline 11 are connected, the other side of the coil of transmission pipeline 11.

preferably, a one-way air inlet valve 4 is fixedly arranged on one side of the top end of the incubator 1, a one-way air outlet valve 5 is fixedly arranged on the other side of the top end of the incubator 1, the air composition in the incubator 1 can be adjusted, the survival rate of cells is improved, a handle is fixedly arranged on one side of the top end of the turntable 6, the turntable 6 can be conveniently rotated, anti-skidding cushion blocks are fixedly arranged on four corners of the bottom end of the water tank 9, the culture device can be prevented from sliding, an observation window 13 is fixedly arranged in the middle of the front face of the incubator door 12, workers can conveniently observe the conditions in the incubator 1 in real time, a metal strip 14 is fixedly arranged on one side of the front face of the incubator door 12, an electromagnet 15 is fixedly arranged on one side of the front face of the incubator 1, the electromagnet 15 is in adsorption connection with the metal strip 14, the incubator 1 is conveniently sealed, an electromagnet switch, a heating pump switch and a cooling pump switch are fixedly arranged in the middle of one side of the incubator 1, the electromagnet 15, the heating pump 18 and the cooling pump 19 are respectively.

when the device is used, the movable plate 2 is drawn out from the incubator 1, then the petri dish 17 with cells is clamped on the movable plate 2, then the movable plate 2 is placed back into the incubator 1, the cabinet door 12 is closed, meanwhile, the electromagnet switch is opened, the electromagnet 15 is connected with the metal strip 14 in an adsorption manner, so that the incubator 1 is sealed, after the incubator 1 is sealed, a part of air in the incubator 1 is drawn out through the one-way air outlet valve 5, then a certain amount of gas beneficial to cell growth is input into the incubator 1 through the one-way air inlet valve 4, after the gas is introduced, the temperature condition inside the incubator 1 is monitored through the temperature sensor 3 with the model of QAE2140.010, if the temperature in the incubator 1 does not reach the optimal cultivation temperature, the temperature sensor 3 with the model of QAE2140.010 controls the heating pump 18 or the cooling pump 19 to heat or cool water in the water tank 9 through the P L C controller 8 with the model of S7-200, then the YPc controller 8 with the model of S7-200 controls the heating pump 18 or the cooling pump 19 to heat or cool the cooling pump 19 to prevent the YPc 16 from being heated and further showing that the working temperature of the microscope working microscope rotating and the YPc 16 is heated and the microscope rotating to be heated and the observation window to be heated and the observation temperature change of the microscope rotating, the YPc working microscope rotating in the observation process of the observation of the cell culture process of the observation of the cell contamination of the cell growth, the cell culture.

In the description of the present invention, it should be understood that the indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawings, and is only for convenience of description and simplification of description, and does not indicate or imply that the indicated device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the present invention.

In the present invention, unless otherwise explicitly specified or limited, for example, it may be fixedly connected, detachably connected, or integrated; can be mechanically or electrically connected; they may be directly connected or indirectly connected through an intermediate medium, and may be connected through the inside of two elements or in an interaction relationship between two elements, unless otherwise specifically defined, and the specific meaning of the above terms in the present invention will be understood by those skilled in the art according to specific situations.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. the utility model provides a cell constant temperature culture apparatus, includes incubator (1), fly leaf (2) and water tank (9), its characterized in that, the bottom of incubator (1) both sides inner wall is respectively through the both sides sliding connection of square spout with fly leaf (2), the top of fly leaf (2) is connected with the top block of eight culture dishes (17), the middle part on incubator (1) top is rotated with the top of dwang (16) and is connected, the top of dwang (16) and the middle part fixed connection of carousel (6) bottom, the bottom of dwang (16) and the top fixed connection of electron microscope (7), and the position one-to-one of electron microscope (7) sets up with the position of culture dish (17), the top of incubator (1) one side inner wall is fixed and is equipped with temperature sensor (3), the top of incubator (1) one side is fixed and is equipped with P L C controller (8), the bottom mounting of incubator (1) is equipped with water tank (9), the fixed one side of water tank (9) inside bottom is equipped with heat pump (18), the other side of heat pump (10) and heat transmission pipeline (11) and the transmission pipeline (10) and the other side of cooling transmission pipeline (11) and the transmission pipeline (10) are connected the other side of cooling chamber (10) and the transmission pipeline (10) that all interpenetration of the transmission pipeline (10) that heat transmission pipeline (10) and the transmission pipeline (10) that heat the transmission pipeline (10) that heat.

2. A cell culture apparatus according to claim 1, wherein: one side on incubator (1) top is fixed and is equipped with one-way admission valve (4), the opposite side on incubator (1) top is fixed and is equipped with one-way air outlet valve (5).

3. A cell culture apparatus according to claim 1, wherein: and a handle is fixedly arranged on one side of the top end of the rotary table (6).

4. A cell culture apparatus according to claim 1, wherein: four corners of the bottom end of the water tank (9) are all fixedly provided with anti-skidding cushion blocks.

5. A cell culture apparatus according to claim 1, wherein: an observation window (13) is fixedly arranged in the middle of the front surface of the box door (12).

6. A cell culture apparatus according to claim 1, wherein: the incubator is characterized in that a metal strip (14) is fixedly arranged on one side of the front face of the incubator door (12), an electromagnet (15) is fixedly arranged on one side of the front face of the incubator (1), and the electromagnet (15) is connected with the metal strip (14) in an adsorption mode.

7. the cell constant-temperature culture device according to claim 6, wherein an electromagnet switch, a heating pump switch and a cooling pump switch are fixedly arranged at the middle part of one side of the incubator (1), the electromagnet (15), the heating pump (18) and the cooling pump (19) are respectively and electrically connected with an external power supply through the electromagnet switch, the heating pump switch and the cooling pump switch, and the temperature sensor (3), the heating pump (18) and the cooling pump (19) are all electrically connected with the PLC controller (8).