CN113462568B - Human peripheral blood NK cell culture device - Google Patents
Human peripheral blood NK cell culture device Download PDFInfo
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- CN113462568B CN113462568B CN202110922173.9A CN202110922173A CN113462568B CN 113462568 B CN113462568 B CN 113462568B CN 202110922173 A CN202110922173 A CN 202110922173A CN 113462568 B CN113462568 B CN 113462568B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M23/22—Transparent or translucent parts
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention relates to a human peripheral blood NK cell culture device, which comprises: incubator, operating device, placing device and observing device; the observation mechanism includes: the camera comprises a display screen for displaying shooting results, a movable frame, a magnetic plate, a camera for shooting, a cleaning plate and an extension plate; the display screen is fixedly connected to one side end of the incubator; the movable frame is movably arranged in the incubator, and the rear side of the movable frame is tightly attached to the rear side wall of the incubator; one side of the cleaning plate is horizontally and fixedly connected to the inner side wall of the incubator. The invention has the advantages that the incubator does not need to be opened for observation during observation, the interference of external bacteria is prevented, the internal temperature of the incubator is prevented from changing due to the opening, and the energy waste caused by the starting of the temperature regulating equipment is prevented for maintaining the temperature. And the air flow is accelerated to improve the temperature regulation speed during temperature regulation, and the problem of culture influence caused by temperature difference is reduced.
Description
Technical Field
The invention relates to the technical field of medical cell culture equipment, in particular to a human peripheral blood NK cell culture device.
Background
NK cells generally refer to natural killer cells. Natural killer cells are important immune cells of the organism, are not only related to anti-tumor, anti-viral infection and immune regulation, but also participate in the occurrence of hypersensitivity and autoimmune diseases in some cases, and can identify target cells and killing media.
When the existing in-vitro NK cell culture equipment is used, operators are required to observe the culture progress in the culture process, and the culture progress can be observed only by taking out the culture dish in the culture. The external environment will interfere with the environment inside the incubator every time the incubator is opened. Foreign bacteria outside the culture device can easily enter the culture device, so that the pollution risk is increased in the culture process. In order to ensure that the temperature condition inside the culture equipment is in a proper state during culture, the temperature needs to be regulated, and air is generally injected at a point during the air injection during the temperature regulation, because the air inside the culture equipment is basically static, the air fluidity is relatively poor, the temperature of one side is easy to reduce and the temperature of the other side does not reach a proper value during the regulation, thereby causing the environmental difference inside the culture equipment.
Disclosure of Invention
The invention aims to provide a human peripheral blood NK cell culture device for solving the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a human peripheral blood NK cell culture apparatus comprising: incubator, operating device, placement mechanism and observation mechanism. The observation mechanism includes: the camera comprises a display screen for displaying shooting results, a movable frame, a magnetic plate, a camera for shooting, a cleaning plate and an extension plate. The display screen is fixedly connected to one side end of the incubator. The movable frame is movably arranged in the incubator, and the rear side of the movable frame is tightly attached to the rear side wall of the incubator. One side of the cleaning plate is horizontally and fixedly connected to the inner side wall of the incubator, the cleaning cotton is fixedly connected to the top end of the cleaning plate, and the rear side end of the magnetic plate is fixedly connected to the front end of the movable frame. The extension plate is vertically and fixedly connected to the front end of the movable frame. The camera fixed connection is to the bottom of extension board. The camera is connected with the display screen in a wireless way. The placing mechanism is arranged inside the incubator. The placement mechanism comprises: rectangular frame, braced skeleton and standing groove. The rectangular frame is movably connected in the incubator. The supporting framework is fixedly connected to the inner center of the rectangular frame. The standing grooves are uniformly formed in the top end of the supporting framework, the longitudinal number of the standing grooves is the same as that of the cameras, and meanwhile, the longitudinal number of the standing grooves is the same as that of the display screen. The operating device is arranged at the bottom end of the incubator. The operating mechanism comprises: the device comprises a chute, a sliding block and an operation block. The spout level is offered in the front end below of incubator, and slider activity joint is in the inside of spout to the slider is the permanent magnet. The operation block is fixedly connected to the front end of the sliding block.
As a further scheme of the invention: the bottom end of the movable frame is clung to the inner bottom end of the incubator.
As a further scheme of the invention: the upper surface of the cleaning plate and the lower surface of the camera are positioned on the same horizontal plane.
As a further scheme of the invention: the horizontal connecting line with the central point of the horizontal line placing groove and the horizontal connecting line with the central point of the horizontal line camera are positioned in the same vertical plane.
As a further scheme of the invention: the human peripheral blood NK cell culture apparatus further comprises: the fingerprint lock comprises a box door and a fingerprint lock for identifying fingerprints of management personnel. The box door is arranged at the left side of the front end of the incubator through a hinge. The fingerprint lock is fixedly arranged on the right side of the front end of the box door.
As a further scheme of the invention: the human peripheral blood NK cell culture apparatus further comprises: temperature detect switch and dispersion mechanism. The temperature control switch is fixedly arranged at the center of the bottom end inside the incubator. The dispersing mechanism comprises: the air conditioner comprises an air conditioner for adjusting temperature, a horizontal rod, a first connecting pipe, an air duct, a fixed ring, a hollow block, a rotating shaft, a blade plate and a second connecting pipe. The air conditioner is fixedly arranged at the left side end of the incubator. The lower end of the first connecting pipe is fixedly arranged at the top end of the air conditioner. The temperature control switch is electrically connected with the air conditioner. The hollow block is fixed at the center of the top end of the incubator. The other side end of the first connecting pipe is fixed at the left center of the hollow block. The second connecting pipe is fixed at the center of the right side end of the hollow block. The fixed ring is fixed at the other side end of the second connecting pipe. The rotating shaft is movably inserted in the center of the hollow block. The blade plate is uniformly fixed at the outer side end of the rotating shaft in a surrounding way. The lower end of the rotating shaft movably passes through the center of the fixed ring. The horizontal rod is horizontally fixed at the lower end of the rotating shaft, and the lower end of the horizontal rod is fixedly provided with a spray head. The wind groove is arranged at the inner center of the horizontal rod. The wind groove is communicated with the inside of the fixed ring.
As a further scheme of the invention: the model of the temperature control switch is KSD9700.
As a further scheme of the invention: the retaining ring further comprises: a hole groove and a rubber ring. The hole slot is arranged at the center of the fixed ring. The rubber ring is fixed inside the hole groove.
As a further scheme of the invention: the inner diameter of the rubber ring is smaller than the outer diameter of the rotating shaft.
As a further scheme of the invention: the first connecting pipe is a horn pipe.
Compared with the prior art, the invention has the beneficial effects that: during observation, the incubator is not required to be opened for observation, so that the interference of external mixed bacteria is prevented, the internal temperature of the incubator is prevented from being changed due to the opening, and in order to maintain the temperature, the energy waste caused by the starting of the temperature regulating equipment is avoided. And the air flow is accelerated to improve the temperature regulation speed during temperature regulation, and the problem of culture influence caused by temperature difference is reduced.
Lay the culture dish inside the standing groove, then with the inside of rectangle frame activity penetration incubator cultivate the work, when needs observe the cultivation state, the user passes through the operation piece and follows the slider through the spout slip slider, drive movable frame under the magnetic force and remove, until the camera shoots culture dish picture display on the display screen, operating personnel observes the work through the display screen, need not to open the incubator and take out the observation, thereby the internal environment of incubator is changed by external influence when preventing to open, the cultivation problem of causing, and remove camera to clean board upper end after observing, clean the camera, prevent that atomizing steam from being infected with and influence the observation on the camera mirror surface.
The fingerprint identification of administrator stores in fingerprint lock, then can only open work by the administrator, prevents the incubator by irrelevant personnel open the cultivation problem that causes.
When the temperature control switch senses that the temperature in the incubator exceeds or is lower than a threshold value during use, the temperature control switch starts an air conditioner. Air-conditioning air is injected into the hollow block through the first connecting pipe, and the first connecting pipe is in a horn shape, so that the passing air can be accelerated, and the impact force of the air is improved. When air enters the hollow block, the blade plate is impacted, so that the blade plate drives the rotating shaft to rotate, and the rotating shaft drives the horizontal rod to rotate, so that the air in the incubator is stirred. And the rubber ring is used for simply and movably sealing the contact between the rubber ring and the rotating shaft when the rotating shaft rotates, so that the gas leakage of the temperature adjustment is prevented. Inside the fixed ring is annotated through the inside air injection of second connecting pipe to the inside hollow piece, then inside the horizontal pole is annotated through the wind groove, and the blowout carries out temperature regulation work to the inside of incubator at last, rotates the inside air flow that the blowout can drive the incubator, improves the speed of adjusting temperature to effectively prevent when the air of temperature regulation gets into the inside of incubator through one side, the accumulation is in one side of infusing, leads to whole incubator temperature not evenly regulated.
Other features and advantages of the present invention will be disclosed in the following detailed description of the invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram showing the structure of a human peripheral blood NK cell culture device of the present invention;
FIG. 2 is a cross-sectional view of the human peripheral blood NK cell culture device of FIG. 1;
FIG. 3 is an enlarged schematic view of the structure A1 of FIG. 2;
FIG. 4 is a left side cross section of the human peripheral blood NK cell culture device of FIG. 1;
FIG. 5 is a top view of the placement mechanism of the human peripheral blood NK cell culture device of FIG. 2.
List of reference numerals: a human peripheral blood NK cell culture device 100; an incubator 10; a door 20; a fingerprint lock 30; an operating mechanism 40; a chute 41; a slider 42; an operation block 43; a placement mechanism 50; a placement groove 51; a rectangular frame 52; a supporting frame 53; a temperature control switch 60; an observation mechanism 70; a display screen 71; a movable frame 72; a magnetic plate 73; a camera 74; a cleaning plate 75; an extension plate 76; a dispersing mechanism 80; an air conditioner 81; a horizontal bar 82; a first connection pipe 83; a wind groove 84; a fixing ring 85; a hollow block 86; a rotation shaft 87; a vane 88; and a second connection pipe 89.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
As shown in fig. 1 to 5, in an embodiment of the present invention, a human peripheral blood NK cell culture apparatus 100 includes: incubator 10, operating mechanism 40, placement mechanism 50, and observation mechanism 70. The observation mechanism 70 includes: a display screen 71 for displaying photographing results, a movable frame 72, a magnetic plate 73, a camera 74 for photographing, a cleaning plate 75, and an extension plate 76. The display screen 71 is fixedly connected to one side end of the incubator 10. The movable frame 72 is movably installed inside the incubator 10, and the rear side of the movable frame 72 is closely attached to the inner rear side wall of the incubator 10. One side of the cleaning plate 75 is horizontally and fixedly connected to the inner side wall of the incubator 10, and cleaning cotton is fixedly connected to the top end of the cleaning plate 75, the rear side end of the magnetic plate 73 is fixedly connected to the front end of the movable frame 72, and the bottom end of the movable frame 72 is tightly attached to the inner bottom end of the incubator 10. The extension plate 76 is vertically fixedly coupled to the front end of the movable frame 72. The camera 74 is fixedly connected to the bottom end of the extension plate 76. The upper surface of the cleaning plate 75 is on the same level as the lower surface of the camera 74. The camera 74 is wirelessly connected to the display screen 71. The placement mechanism 50 is provided inside the incubator 10. The placement mechanism 50 includes: a rectangular frame 52, a supporting skeleton 53, and a placement groove 51. The rectangular frame 52 is movably connected inside the incubator 10. The supporting frame 53 is fixedly coupled to the inner center of the rectangular frame 52. The placement grooves 51 are uniformly formed in the top end of the supporting frame 53, the number of the placement grooves 51 is the same as the number of the cameras 74 in the longitudinal direction, and the number of the placement grooves 51 is the same as the number of the display screens 71 in the longitudinal direction. The horizontal connecting line with the center point of the horizontal line placing groove 51 and the horizontal connecting line with the center point of the horizontal line camera 74 are located in the same vertical plane. An operating mechanism 40 is provided at the inner bottom end of the incubator 10. The operating mechanism 40 includes: a chute 41, a slider 42 and an operating block 43. The sliding groove 41 is horizontally arranged below the front end of the incubator 10, the sliding block 42 is movably clamped in the sliding groove 41, and the sliding block 42 is a permanent magnet. The operation block 43 is fixedly connected to the front end of the slider 42.
The culture dish is placed inside the standing groove 51 during cultivation, then the rectangular frame 52 is movably penetrated into the incubator 10 to perform cultivation work, when the cultivation state is required to be observed, a user slides the sliding block 42 along the sliding groove 41 through the operation block 43, the movable frame 72 is driven to move along the sliding block 42 under the action of magnetic force until the camera 74 shoots the culture dish picture to be displayed on the display screen 71, an operator performs the observation work through the display screen 71, and does not need to open the incubator 10 to take out the observation, so that the internal environment of the incubator 10 is prevented from being changed by external influences during the opening, the caused cultivation problem is solved, the camera 74 is moved to the upper end of the cleaning plate 75 after the observation is completed, the camera 74 is cleaned, and atomized water vapor is prevented from being stained on the mirror surface of the camera 74 to influence the observation.
The human peripheral blood NK cell culture apparatus 100 further comprises: a door 20 and a fingerprint lock 30 for identifying a manager's fingerprint. Door 20 is hinged to the left side of the front end of incubator 10. Fingerprint lock 30 is fixedly mounted on the right side of the front end of door 20.
When in use, the fingerprint identification of the manager is stored in the fingerprint lock 30, and then the manager can only open the incubator 10, so that the incubator 10 can be prevented from being opened by irrelevant people to cause incubation problems.
The human peripheral blood NK cell culture apparatus 100 further comprises: a temperature controlled switch 60 and a dispersion mechanism 80. The temperature control switch 60 is fixedly arranged at the center of the inner bottom end of the incubator 10, and the model of the temperature control switch 60 is KSD9700. The dispersing mechanism 80 includes: the air conditioner 81 for adjusting temperature, the horizontal bar 82, the first connection pipe 83, the air duct 84, the fixing ring 85, the hollow block 86, the rotation shaft 87, the vane 88, and the second connection pipe 89. An air conditioner 81 is fixedly installed at the left side end of the incubator 10. The lower end of the first connecting pipe 83 is fixedly arranged at the top end of the air conditioner 81, and the first connecting pipe 83 is a horn pipe. The temperature control switch 60 is electrically connected to the air conditioner 81. Hollow block 86 is fixed to the center of the top end of incubator 10. The other end of the first connecting tube 83 is fixed to the left center of the hollow block 86. A second connection pipe 89 is fixed to the right-side end center of the hollow block 86. The fixing ring 85 is fixed to the other side end of the second connection pipe 89. The rotation shaft 87 is movably inserted in the center of the hollow block 86. The louver 88 is uniformly fixed around the outer end of the rotating shaft 87. The lower end of the rotation shaft 87 is movably passed through the center of the fixing ring 85. The horizontal rod 82 is horizontally fixed to the lower end of the rotating shaft 87, and a spray head is fixed to the lower end of the horizontal rod 82. A wind groove 84 is provided at the inner center of the horizontal bar 82. The air groove 84 communicates with the inside of the fixing ring 85.
The fixing ring 85 further includes: a hole slot 852 and a rubber ring 851. A slot 852 is provided in the center of the securing ring 85. The rubber ring 851 is fixed inside the hole groove 852. The inner diameter of the rubber ring 851 is smaller than the outer diameter of the rotary shaft 87.
When the temperature inside incubator 10 exceeds or falls below a threshold value, temperature control switch 60 activates air conditioner 81. Air of the air conditioner 81 is injected into the inside of the hollow block 86 through the first connection pipe 83, and since the first connection pipe 83 is horn-shaped, the passing air is accelerated, and the impact force of the air is improved. When air enters the hollow block 86 and then impacts the blade 88, the blade 88 drives the rotating shaft 87 to rotate, and the rotating shaft 87 drives the horizontal rod 82 to rotate, so that the air in the incubator 10 is stirred. And when the rotating shaft 87 rotates, the rubber ring 852 is utilized to simply and movably seal the rubber ring 852 to be contacted with the rotating shaft 87, so that the gas leakage of the temperature regulation is prevented. Air injected into the hollow block 86 is injected into the fixing ring 85 through the second connecting pipe 89, then is injected into the horizontal rod 82 through the air duct 84, finally is sprayed out to carry out temperature regulation work on the inside of the incubator 10, and the rotating spraying can drive air in the incubator 10 to flow, so that the temperature regulation speed is improved, and when the temperature-regulated air is effectively prevented from entering the inside of the incubator 10 through one side, accumulation is prevented from being carried out on one side of injection, so that the temperature of the whole incubator 10 is not uniformly regulated.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
Claims (5)
1. A human peripheral blood NK cell culture device is characterized in that,
the device comprises an incubator, an operating mechanism, a placing mechanism, an observing mechanism, a dispersing mechanism and a temperature control switch; wherein, the liquid crystal display device comprises a liquid crystal display device,
the observation mechanism includes: the camera comprises a display screen for displaying shooting results, a movable frame, a magnetic plate, a camera for shooting, a cleaning plate and an extension plate; the display screen is fixedly connected to one side end of the incubator, and the camera is in wireless connection with the display screen; the movable frame is movably arranged in the incubator, the rear side of the movable frame is clung to the rear side wall of the incubator, and the bottom end of the movable frame is clung to the bottom end of the incubator; the rear side end of the magnetic plate is fixedly connected to the front end of the movable frame; one side of the cleaning plate is horizontally and fixedly connected to the inner side wall of the incubator, cleaning cotton is fixedly connected to the top end of the cleaning plate, and the upper surface of the cleaning plate and the lower surface of the camera are positioned on the same horizontal plane; the extension plate is vertically and fixedly connected to the front end of the movable frame; the camera is fixedly connected to the bottom end of the extension plate;
the placing mechanism is arranged in the incubator; the placement mechanism comprises a rectangular frame, a supporting framework and a placement groove; the rectangular frame is movably connected inside the incubator; the supporting framework is fixedly connected to the inner center of the rectangular frame; the placing grooves are uniformly formed in the top end of the supporting framework, the longitudinal number of the placing grooves is the same as that of the cameras, and the longitudinal number of the placing grooves is the same as that of the display screen; the horizontal connecting line of the central point of the placing groove and the horizontal connecting line of the central point of the camera are positioned in the same vertical plane;
the operating mechanism is arranged at the bottom end of the interior of the incubator; the operating mechanism comprises a chute, a sliding block and an operating block; the sliding groove is horizontally arranged below the front end of the incubator, the sliding block is movably clamped in the sliding groove, and the sliding block is a permanent magnet; the operation block is fixedly connected to the front end of the sliding block;
the dispersing mechanism includes: the air conditioner comprises an air conditioner for adjusting temperature, a horizontal rod, a first connecting pipe, an air duct, a fixed ring, a hollow block, a rotating shaft, a blade plate and a second connecting pipe; the air conditioner is fixedly arranged at the left side end of the incubator; the lower end of the first connecting pipe is fixedly arranged at the top end of the air conditioner; the hollow block is fixed at the center of the top end of the incubator; the other side end of the first connecting pipe is fixed at the left center of the hollow block; the second connecting pipe is fixed at the center of the right side end of the hollow block; the fixed ring is fixed at the other side end of the second connecting pipe; the rotating shaft is movably inserted in the center of the hollow block; the blade plates are uniformly fixed at the outer side end of the rotating shaft in a surrounding manner; the lower end of the rotating shaft movably passes through the center of the fixed ring; the horizontal rod is horizontally fixed at the lower end of the rotating shaft, and a spray head is fixed at the lower end of the horizontal rod; the air groove is formed in the center of the inner part of the horizontal rod; the air groove is communicated with the inside of the fixed ring;
the temperature control switch is fixedly arranged at the center of the bottom end of the interior of the incubator; the temperature control switch is electrically connected with the air conditioner;
the working process of the human peripheral blood NK cell culture device comprises the following steps: placing a culture dish in the placing groove, movably penetrating the rectangular frame into the incubator, sliding the sliding block along the sliding groove through the operation block when the culture state is required to be observed, driving the movable frame to move along with the sliding block under the action of magnetic force, and moving the camera to the upper end of the cleaning plate after the observation is completed so as to clean the camera; the temperature control switch is used for inducing the internal temperature of the incubator to exceed or be lower than a threshold value, air conditioner air is injected into the hollow block through the first connecting pipe, the blade plate is impacted, the blade plate is enabled to drive the rotating shaft to rotate, the rotating shaft drives the horizontal rod to rotate, air injected into the hollow block is injected into the fixing ring through the second connecting pipe, and the air is injected into the horizontal rod through the air duct and then sprayed out.
2. The human peripheral blood NK cell culture apparatus of claim 1, further comprising a door and a fingerprint lock for identifying a manager's fingerprint; the box door is arranged at the left side of the front end of the incubator through a hinge; the fingerprint lock is fixedly arranged on the right side of the front end of the box door.
3. The human peripheral blood NK cell culture device of claim 2, wherein the retaining ring further comprises a well and a rubber ring; the hole groove is formed in the center of the fixed ring; the rubber ring is fixed inside the hole groove.
4. A human peripheral blood NK cell culture device according to claim 3, wherein the inner diameter of the rubber ring is smaller than the outer diameter of the shaft.
5. The human peripheral blood NK cell culture device of claim 4, wherein the first connecting tube is a horn tube.
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| CN202110922173.9A CN113462568B (en) | 2021-08-12 | 2021-08-12 | Human peripheral blood NK cell culture device |
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| CN202110922173.9A CN113462568B (en) | 2021-08-12 | 2021-08-12 | Human peripheral blood NK cell culture device |
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| CN114181832A (en) * | 2021-12-23 | 2022-03-15 | 内蒙古医科大学附属人民医院(内蒙古自治区肿瘤医院) | Auxiliary device convenient for operating NK cell in-vitro separation culture and use method |
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