CN210900803U - Tissue culture case that alpine rose tissue culture used - Google Patents
Tissue culture case that alpine rose tissue culture used Download PDFInfo
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- CN210900803U CN210900803U CN201921826592.7U CN201921826592U CN210900803U CN 210900803 U CN210900803 U CN 210900803U CN 201921826592 U CN201921826592 U CN 201921826592U CN 210900803 U CN210900803 U CN 210900803U
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Abstract
The utility model belongs to the technical field of biological culture devices, and discloses a tissue culture box for alpine rhododendron tissue culture, which comprises a box body and a box door, wherein the inner cavity of the box body is provided with a first clapboard and a second clapboard, a first cavity is formed by the first partition plate and the second partition plate, a second cavity and a third cavity are formed by the second partition plate and the first partition plate, the top wall of each cavity is provided with an LED lamp strip with adjustable brightness, the LED lamp strip is provided with an astigmatic lampshade, the middle part of the second partition plate is provided with a central cavity with a temperature and humidity controller, first through holes are formed in the left side wall, the right side wall, the upper side wall and the lower side wall of the central cavity, a ventilation pipeline is arranged on the rear side of the central cavity, a fan and a first air filter screen are arranged in the ventilation pipeline, spiral blades are arranged in the ventilation pipeline between the air filter screen and the central cavity, an exhaust pipeline with a lower end communicated with the second. The device ensures effective circulation of air in the box body, and realizes effective regulation and control of culture conditions in the box body.
Description
Technical Field
The utility model relates to a biological culture device technical field specifically is a tissue culture case that alpine rose tissue culture used.
Background
Rhododendron lapponicum is a Rhododendron plant in Ericaceae (Ericaceae), has the characteristics of various varieties, gorgeous flowers, beautiful posture, unique landscape and the like, and has high ornamental value. The leaves can extract spice, and the tanned matter in the bark can extract tannin extract. The tannin extract can be used as natural air cleaner and also can be used for light industrial production. Many rhododendrons also have high medical value and have the curative effects of promoting blood circulation, relieving pain and dispelling wind.
The alpine rhododendron usually adopts several methods of sowing, cuttage, layering, grafting, tissue culture and the like, wherein seeds are not needed in the tissue culture, a large number of rhododendron seedlings can be cultured in a test tube in a short time as long as stem tips of newly-grown branches of the alpine rhododendron are grown, and the alpine rhododendron is not limited by seasons and has wide application prospect.
The prior art carries out tissue culture on alpine rhododendron, does not have a special tissue culture box for alpine rhododendron, and culture conditions such as illumination, temperature, ventilation and the like cannot be controlled in a targeted manner, so that the tissue culture of rhododendron is not facilitated.
SUMMERY OF THE UTILITY MODEL
The utility model provides a tissue culture case that alpine rose tissue culture used solves the tissue culture case that prior art used and can not have corresponding regulation and control culture conditions such as illumination, temperature, ventilation, is unfavorable for alpine rose's tissue culture's problem.
In order to achieve the above object, the present invention provides the basic scheme that: the utility model provides a tissue culture case that alpine rose tissue culture used, chamber door including box and front side, the inner chamber of box is equipped with first baffle and second baffle, and just first baffle is located the top of second baffle, the box inner chamber is formed with first cavity, second cavity and third cavity through first baffle and second baffle, the roof of first cavity, second cavity and third cavity all is provided with the led lamp area of adjustable luminance, led lamp area is provided with the astigmatism lamp shade, the vertical two plywoods and the third chamber of being provided with in middle part of second baffleBe formed with central cavity, a plurality of first through-holes have all been seted up to lateral wall about the central cavity and upper and lower lateral wall, the lateral wall of central cavity is provided with temperature and humidity controller, the rear side of box is provided with the air pipe with central cavity intercommunication, fan and first air filter net have set gradually in the air pipe, be provided with the heliciform blade in the air pipe between air filter net to the central cavity, be provided with the exhaust duct that the lower extreme link up in the lateral wall about the box, exhaust duct is provided with the second through-hole with each cavity intercommunication of box, exhaust duct's lower extreme is provided with second air filter net.
Working principle of basic scheme: the tissue culture of alpine rhododendron mainly comprises three steps of initial induction culture, multiplication culture and rooting culture, the illumination intensity and the temperature are approximately the same, but the illumination time and the culture period are inconsistent, the device mainly divides a box body into three chambers, culture dishes containing different culture mediums in the three steps are sequentially placed, each chamber is provided with an led lamp strip with adjustable brightness, the led lamp strip can be respectively regulated and controlled according to the required illumination time and illumination intensity, the central chamber is provided with a temperature and humidity controller and is communicated with a ventilation pipeline, external air filtered by an air filter screen enters the central chamber from the ventilation pipeline, the temperature and humidity of the air are regulated and controlled by the temperature and humidity controller, then the air is scattered from first through holes on the side wall of the central chamber and enters each chamber, and finally the air enters an exhaust pipeline to be exhausted, so that the circulating ventilation is realized.
The basic scheme has the advantages that: (1) this tissue culture case is divided into three cavities of placing the culture dish on the whole, corresponds alpine rhododendron tissue culture's three main step respectively, makes things convenient for the staff to carry out concrete operation to every step, avoids the tissue culture chronogenesis confusion of each culture dish, leads to the tissue culture failure. (2) This tissue culture case sets up air pipe and atmospheric control ware through the middle part, and is provided with the heliciform blade in the air pipe, makes the production whirl of admitting air, and even scattered each cavity that gets into all around makes the ventilation and the humiture uniformity of each layer in the box good, ensures the effective circulation of air, realizes the effective regulation and control to the interior condition of cultivateing of box. (3) LED lamp strip of tissue culture boxThe setting up make its illumination coverage area big, and the astigmatism lamp shade makes the illumination intensity distribution in the box even, has guaranteed illumination and the good control of illumination intensity among the whole tissue culture process.
Further, the method can be used for preparing a novel materialThe side wall of the box body comprises an outer layer and an inner container layer, a heat insulation layer is arranged between the outer layer and the inner container layer, the inner container layer is made of stainless steel, and the heat insulation layer is made of glass fiber cotton plates.
Through the arrangement, the inner container layer is made of stainless steel, is convenient to clean and not easy to damage, and the thermal insulation layer ensures the temperature control effect of the tissue culture box.
Further, the method can be used for preparing a novel materialA plurality of first chutes are formed in the upper surfaces of the first partition plate and the second partition plate, the front ends of the first chutes are communicated, guide protruding strips are arranged on the left side wall and the right side wall of each first chute, and the first chutes are connected with the storage plates in a sliding mode.
Through the setting, when the tissue culture condition of a certain culture dish needs to be observed alone to the staff, because of every cavity space is limited, the actual conditions of culture dish can't be observed to the omnidirectional, need take out the culture dish, and this tissue culture case then can directly pull out the part and put the thing board, and convenient observation need not the back and forth movement culture dish and influences the tissue culture of alpine rhododendron.
Further, the method can be used for preparing a novel materialThe utility model discloses a storage plate, including the upper surface of putting the thing board, the upper surface of putting the thing board is provided with the arc blend stop of two bilateral symmetry, and wherein the arc blend stop bottom surface rigid coupling on right side has the slider, put the right side of thing board and seted up horizontal second spout, slider and second spout sliding connection, the rigid coupling has the spring between the right-hand member of slider and second spout, put the front end of thing board and seted up the third spout that communicates with the second spout, third spout sliding connection has the control lever, the rear end and the slider fixed connection of control lever, the third spout is stretched out to the front end of control lever.
Through the aforesaid setting, the culture dish is lived to operatable arc blend stop centre gripping, avoids influencing alpine rhododendron tissue culture because of rocking the collision impaired between each culture dish, has also guaranteed directly to shift out and has put the thing board, pollutes operations such as necrotic tissue processing, puts back the steadiness of putting the culture dish of this process of thing board again, avoids sliding and drops.
Further, the method can be used for preparing a novel materialAnd the concave side of the arc-shaped barrier strip is provided with an elastic sponge layer.
Through the arrangement, the culture dish is prevented from being scratched when the arc-shaped barrier strips are clamped.
Drawings
FIG. 1 is a schematic structural view of a tissue culture box for tissue culture of alpine rhododendron of the present invention;
FIG. 2 is a left side view of a tissue culture box for tissue culture of alpine rhododendron of the present invention;
fig. 3 is a top view of the shelf of fig. 1.
Detailed Description
The following is further detailed by the specific embodiments:
reference numerals in the drawings of the specification include: the LED lamp comprises a box body 1, a first partition plate 2, a second partition plate 3, an LED lamp strip 4, an astigmatism lampshade 5, a laminate plate 6, a first through hole 7, a temperature and humidity controller 8, a fan 9, a first air filter screen 10, a spiral blade 11, an exhaust duct 12, a second through hole 13, a second air filter screen 14, an object placing plate 15, an arc-shaped barrier strip 16, a second chute 17, a third chute 18, a control rod 19 and a spring 20.
The embodiment is as shown in fig. 1, a tissue culture box for tissue culture of alpine rhododendron comprises a box body 1 and a box door on the front side, the side wall of the box body 1 comprises an outer layer and an inner container layer, a heat insulation layer is arranged between the outer layer and the inner container layer, the inner container layer is made of stainless steel and is made of glass fiber cotton plates, a first partition plate 2 and a second partition plate 3 are arranged in an inner cavity of the box body 1, the first partition plate 2 is located above the second partition plate 3, and a first chamber, a second chamber and a third chamber are formed in the inner cavity of the box body 1 through the first partition plate 2 and the second partition plate 3.
The top walls of the first chamber, the second chamber and the third chamber are transversely provided with LED lamp belts 4 with adjustable brightness, the LED lamp belts 4 are provided with light diffusion lamp shades 5, the middle part of the second clapboard 3 is vertically welded with two laminated plates 6, a central chamber is formed between the two laminated plates 6 and the first clapboard 2 as well as the rear side wall of the box body 1, the left and right side walls and the upper and lower side walls of the central chamber are respectively provided with a plurality of first through holes 7, the lower side wall of the central chamber is provided with a temperature and humidity controller 8 which adopts a Miao Xin brand TH110 high-precision temperature and humidity controller 8 and is electrically connected with a power supply, referring to figure 2, the rear side of the box body 1 is provided with a ventilating duct communicated with the central chamber, a fan 9, an ultraviolet sterilizing lamp and a first air filter screen 10 are sequentially arranged in the ventilating duct from the rear to the front, two spiral blades 11 are arranged in the ventilating duct between, the spiral blades 11 generate rotational flow to enter the central cavity, an exhaust duct 12 with a through lower end is laid in the left side wall and the right side wall of the box body 1, second through holes 13 communicated with the cavities of the box body 1 are arranged in the exhaust duct 12, and a second air filter 14 is arranged at the lower end of the exhaust duct 12.
Referring to fig. 3, the upper surfaces of the first partition board 2 and the second partition board 3 are respectively provided with two first sliding grooves, the front ends of the first sliding grooves are through, and the left and right side walls of the first sliding grooves are respectively integrally formed with a guiding protruding strip, the first sliding grooves are slidably connected with a storage board 15, the upper surface of the storage board 15 is provided with two arc-shaped retaining strips 16 which are bilaterally symmetrical, the concave side of each arc-shaped retaining strip 16 is provided with an elastic sponge layer, the bottom surface of the arc-shaped retaining strip 16 on the right side is fixedly welded with a sliding block, the right side of the storage board 15 is provided with a transverse second sliding groove 17, the sliding block is slidably connected with the second sliding groove 17, a spring 20 is fixedly connected between the sliding block and the right end of the second sliding groove 17, the front end of the storage board 15 is provided with a third sliding groove 18 communicated with the second sliding groove 17, the third sliding groove 18 is slidably connected with a control rod 19, and the front end of the control rod 19 is flush with the front ends of the left and right side walls of the box body 1.
In the specific work, the tissue culture of alpine rhododendron mainly comprises three steps of initial induction culture, proliferation culture and rooting culture, the illumination intensity and the temperature are approximately same, but the illumination time and the culture period are not consistent, the device divides a box body 1 into three chambers, culture dishes containing different culture mediums in the three steps are placed in sequence, namely the initial induction culture medium, the proliferation culture medium and the rooting culture medium, concretely, a storage plate 15 is taken down, a control rod 19 is slid to enable an arc-shaped barrier strip 16 on the right side to move to the right, the culture dish is placed on the upper side of the storage plate 15 and is abutted against an arc-shaped barrier strip 16 on the left side, then the hand is slowly released, the arc-shaped barrier strip 16 on the right side moves to the left side and is matched with the arc-shaped barrier strip 16 on the left side to clamp the culture dish, and then the LED 4 brightness and the illumination time of each chamber are respectively regulated and controlled according to the, the blower 9 and the temperature and humidity controller 8 of the central cavity are started, outside air filtered by the air filter screen enters the central cavity from the ventilating duct, the temperature and the humidity of the air are regulated and controlled by the temperature and humidity controller 8, then the outside air is scattered from the first through holes 7 on the side wall of the central cavity and enters each cavity, finally the outside air enters the exhaust duct to be discharged, circulating ventilation is realized, if tissue culture conditions in a culture medium need to be observed, a box door can be opened, and a corresponding object placing plate 15 can be pulled out.
The above embodiments of the present invention are only examples, and the common knowledge of the known specific structures and characteristics in the schemes is not described too much, it should be noted that, for those skilled in the art, without departing from the structure of the present invention, several modifications and improvements can be made, and these should be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (5)
1. The utility model provides a tissue culture case that alpine rose tissue culture used, includes the chamber door of box and front side, its characterized in that: the inner cavity of the box body is provided with a first partition plate and a second partition plate, the first partition plate is positioned above the second partition plate, the inner cavity of the box body is provided with a first cavity, a second cavity and a third cavity through the first partition plate and the second partition plate, the top walls of the first cavity, the second cavity and the third cavity are all provided with LED lamp belts with adjustable brightness, the LED lamp belts are provided with astigmatic lampshade, the middle part of the second partition plate is vertically provided with two laminated plates and forms a central cavity, the left side wall, the right side wall, the upper side wall and the lower side wall of the central cavity are respectively provided with a plurality of first through holes, the side wall of the central cavity is provided with a temperature and humidity controller, the rear side of the box body is provided with a ventilation pipeline communicated with the central cavity, a fan and a first air filter screen are sequentially arranged in the ventilation pipeline between the air filter screen, an exhaust duct with a through lower end is arranged in the left side wall and the right side wall of the box body, the exhaust duct is provided with second through holes communicated with the chambers of the box body, and a second air filter screen is arranged at the lower end of the exhaust duct.
2. The tissue culture box for rhododendron lapponicum tissue culture according to claim 1, wherein: the side wall of the box body comprises an outer layer and an inner container layer, a heat insulation layer is arranged between the outer layer and the inner container layer, the inner container layer is made of stainless steel, and the heat insulation layer is made of glass fiber cotton plates.
3. The tissue culture box for rhododendron lapponicum tissue culture according to claim 1, wherein: a plurality of first spouts have all been seted up to first baffle and second baffle upper surface, the front end of first spout link up, and the lateral wall all is provided with the direction sand grip about first spout, first spout sliding connection has puts the thing board.
4. The tissue culture box for rhododendron lapponicum tissue culture according to claim 3, wherein: the upper surface of putting the thing board is provided with the arc blend stop of two bilateral symmetry, and wherein the arc blend stop bottom surface rigid coupling on right side has the slider, put the right side of thing board and seted up horizontal second spout, slider and second spout sliding connection, the rigid coupling has the spring between the right-hand member of slider and second spout, put the front end of thing board and seted up the third spout that communicates with the second spout, third spout sliding connection has the control lever, the rear end and the slider fixed connection of control lever, the third spout is stretched out to the front end of control lever.
5. The tissue culture box for rhododendron lapponicum tissue culture according to claim 4, wherein: the concave side of the arc-shaped barrier strip is provided with an elastic sponge layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921826592.7U CN210900803U (en) | 2019-10-29 | 2019-10-29 | Tissue culture case that alpine rose tissue culture used |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921826592.7U CN210900803U (en) | 2019-10-29 | 2019-10-29 | Tissue culture case that alpine rose tissue culture used |
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| Publication Number | Publication Date |
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| CN210900803U true CN210900803U (en) | 2020-07-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201921826592.7U Expired - Fee Related CN210900803U (en) | 2019-10-29 | 2019-10-29 | Tissue culture case that alpine rose tissue culture used |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778160A (en) * | 2020-07-21 | 2020-10-16 | 四川省人民医院 | Cell culture device |
| CN113462568A (en) * | 2021-08-12 | 2021-10-01 | 杭州中赢生物医疗科技有限公司 | Human peripheral blood NK cell culture apparatus |
-
2019
- 2019-10-29 CN CN201921826592.7U patent/CN210900803U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778160A (en) * | 2020-07-21 | 2020-10-16 | 四川省人民医院 | Cell culture device |
| CN113462568A (en) * | 2021-08-12 | 2021-10-01 | 杭州中赢生物医疗科技有限公司 | Human peripheral blood NK cell culture apparatus |
| CN113462568B (en) * | 2021-08-12 | 2023-07-28 | 杭州中赢生物医疗科技有限公司 | Human peripheral blood NK cell culture device |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200703 Termination date: 20211029 |
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| CF01 | Termination of patent right due to non-payment of annual fee |