CN209702757U - A kind of plate streaking selects the automatic device of purebred bacterium colony - Google Patents
A kind of plate streaking selects the automatic device of purebred bacterium colony Download PDFInfo
- Publication number
- CN209702757U CN209702757U CN201920401835.6U CN201920401835U CN209702757U CN 209702757 U CN209702757 U CN 209702757U CN 201920401835 U CN201920401835 U CN 201920401835U CN 209702757 U CN209702757 U CN 209702757U
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- CN
- China
- Prior art keywords
- sterilization box
- microwave sterilization
- telescopic rod
- electron microscope
- microwave disinfection
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 241000894006 Bacteria Species 0.000 title abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 58
- 230000001954 sterilising effect Effects 0.000 claims description 51
- 230000001580 bacterial effect Effects 0.000 claims description 29
- 239000001963 growth medium Substances 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 238000004500 asepsis Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
A kind of plate streaking selects the automatic device of purebred bacterium colony, the brake apparatus including the sterile microwave disinfection case of constant temperature, the automatic identification equipment in energy complete observation microwave disinfection bottom portion and achievable colony lift and plate streaking；Automatic identification equipment is located at microwave disinfection box top, and brake apparatus is fixed on microwave disinfection case medial surface；The automatic identification equipment includes electron microscope, the cell potential transducer of the recognizable bacterium colony in electron microscope end and the touch screen of microwave disinfection case upper surface inside microwave disinfection case, and electron microscope is connect with touch screen telecommunications；The brake apparatus include one end be fixed on microwave disinfection case side sliding rail and can side-to-side movement on the slide rail mechanical arm, mechanical arm is slidably installed on the slide rail；The utility model improves the success rate of experiment, reduces the probability for causing mistake because of people, reduce resource waste compared with previous technology.
Description
Technical Field
The utility model belongs to the technical field of the automatic technique and specifically relates to an automatic device for pure bacterial colony is selected in dull and stereotyped marking off.
Background
In the prior art, when pure colonies for culturing microorganisms are selected, the plate streaking method is troublesome in operation, the selection operation steps are complicated, and manual operation is inevitable and has some errors.
SUMMERY OF THE UTILITY MODEL
To the foregoing, the utility model provides an automatic device of pure kind of bacterial colony is selected in dull and stereotyped rule to solve above-mentioned some problems that appear under the existing condition.
According to one aspect of the utility model, an automatic device for selecting pure bacterial colonies by flat plate lineation is provided, which comprises a constant temperature aseptic microwave sterilization box, an automatic identification device capable of comprehensively observing the bottom of the microwave sterilization box and a brake device capable of completing bacterial colony transfer and flat plate lineation; the automatic identification device is positioned at the top of the microwave sterilization box, and the brake device is fixed on the inner side surface of the microwave sterilization box; the automatic identification device comprises an electron microscope positioned in the microwave sterilization box, a cell potential sensor at the tail end of the electron microscope and capable of identifying bacterial colonies, and a touch screen on the upper end surface of the microwave sterilization box, wherein the electron microscope is electrically connected with the touch screen; the braking device comprises a slide rail with one end fixed on the side surface of the microwave sterilization box and a mechanical arm capable of moving left and right on the slide rail, and the mechanical arm is slidably arranged on the slide rail; wherein,
the upper end surface of the microwave sterilization box is provided with a microcontroller, and the microcontroller is electrically connected with the mechanical arm and the automatic identification device;
the type of the micro controller is a single chip microcomputer of AT89C2051 or TMS320VC5509A and the like;
a culture dish is placed at the bottom of the microwave sterilization box, and a solid culture medium with a flat plate line is filled in the culture dish.
Preferably, the mechanical arm comprises a stepping motor on the sliding rail, a rotating shaft, a first micro motor on the rotating shaft, a horizontal telescopic rod, a second micro motor arranged in the tail end of the horizontal telescopic rod, a vertical telescopic rod, a third micro motor arranged in the tail end of the vertical telescopic rod and a mechanical disc; the stepping motor is arranged on the slide rail and can move left and right, the rotating shaft is arranged above the stepping motor, the horizontal telescopic rod is arranged on the rotating shaft and is connected with the first micro motor, and the vertical telescopic rod is vertically arranged at the tail end of the horizontal telescopic rod and is connected with the second micro motor; the mechanical disc is obliquely arranged at the tail end of the vertical telescopic rod and is connected with one end of a third micro motor.
Preferably, the mechanical disc is provided with a bacterial colony suction pipe and a inoculating loop for plate scribing, the inoculating loop is vertically arranged on the mechanical disc and is vertical to the bottom surface of the microwave sterilization box, and the bacterial colony suction pipe is obliquely arranged on the mechanical disc and is vertical to the mechanical disc.
Preferably, the electron microscope comprises a lens cone, a spherical lens at the tail end of the lens cone and a support frame fixed on one side of the microwave sterilization box; the lens cone is fixed at the center of the microwave sterilization box by the other end of the support frame, and the spherical lens is detachably arranged at the tail end of the lens cone.
Preferably, one side of the microwave sterilization box is provided with a door body, and the door body is connected to the prism of the microwave sterilization box through a hinge and is matched with the microwave sterilization box.
The utility model has the advantages that: the whole-process sterile operation increases the success rate of the experiment, avoids errors caused by manual work in a complicated operation process, and has higher mechanical efficiency.
Drawings
FIG. 1 is an overall structure diagram of the present invention;
fig. 2 is a structural view of the brake device.
Detailed Description
The invention will be further explained with reference to the drawings and some embodiments.
In fig. 1-2, an automatic device for selecting pure bacterial colonies by plate streaking comprises a microwave sterilization box (9) with constant temperature and asepsis, an automatic recognition device capable of comprehensively observing the bottom of the microwave sterilization box (9), and a brake device (5) capable of completing bacterial colony transfer and plate streaking; the automatic identification device is positioned at the top of the microwave sterilization box (9), and the brake device (5) is fixed on the inner side surface of the microwave sterilization box (9); the automatic identification device comprises an electron microscope positioned in the microwave sterilization box (9), a cell potential sensor (2) with the tail end capable of identifying bacterial colonies of the electron microscope and a touch screen (4) on the upper end surface of the microwave sterilization box (9); the electron microscope is electrically connected with the touch screen (4); the braking device (5) comprises a sliding rail (501) with one end fixed on the side surface of the microwave sterilization box (9) and a mechanical arm capable of moving left and right on the sliding rail (501), and the mechanical arm is slidably mounted on the sliding rail; wherein, the upper end surface of the microwave sterilization box (9) is provided with a micro controller (8); a culture dish is placed at the bottom of the microwave sterilization box (9); the culture dish is internally provided with a plate-streaked solid culture medium (1).
In the embodiment, the cell potential sensor (2) plays a role in identification and positioning; the microcontroller (8) is a control center of the whole operating system; the micro-controller (8) is electrically connected with the mechanical arm and the automatic identification device, and the micro-controller (8) can be programmed according to the actual running condition of the mechanical arm.
In the embodiment, the electron microscope comprises a lens cone (3), a spherical lens at the tail end of the lens cone (3) and a support frame (7) fixed on one side of a microwave sterilization box (9); the lens cone (3) is fixed at the center of the microwave sterilization box (9) by the other end of the support frame (7), and the spherical lens is detachably arranged at the tail end of the lens cone (3); the spherical lens can observe any position at the bottom of the microwave sterilization box (9), and displays a picture on the touch screen (4) so that an operator can observe the inside of the microwave sterilization box conveniently.
In this embodiment, the robot arm includes a stepping motor (503) on a slide rail, a rotating shaft (502), a first micro motor (5011) on the rotating shaft (501), a horizontal telescopic rod (504), a second micro motor (5010) built in the end of the horizontal telescopic rod (504), a vertical telescopic rod (505), a third micro motor (509) built in the end of the vertical telescopic rod (505), and a mechanical disc (506); the stepping motor (503) is arranged on the sliding rail (501) and can move left and right, the rotating shaft (502) is arranged above the stepping motor (503), the horizontal telescopic rod (504) is arranged on the rotating shaft and is connected with the first micro motor (5011), and the vertical telescopic rod (505) is vertically arranged at the tail end of the horizontal telescopic rod (505) and is connected with the second micro motor (5010); the mechanical disc (506) is obliquely arranged at the tail end of the vertical telescopic rod (505) and is connected with one end of a third micro motor (509); the rotating shaft (502) can control the whole mechanical arm to rotate in 360 degrees, and the first micro motor (5011) controls the horizontal telescopic rod (504) to extend or shorten and controls the position in the horizontal direction; the second micro motor (5010) controls the vertical telescopic rod (505) to descend or ascend, and controls the position in the vertical direction; a third micro motor (509) controls the mechanical disc (506) to rotate; the microcontroller (8) controls the mechanical arm to mark at a fixed position in the center of the bottom of the microwave sterilization box (9) through a program, and the microcontroller (8) receives a position signal of the cell potential sensor (2) to control the mechanical arm to finish the bacterial colony suction process.
In the embodiment, a bacterial colony suction pipe (507) and an inoculating loop (508) for flat plate scribing are arranged on the mechanical disc (506), the inoculating loop is vertically arranged on the mechanical disc (506) and is vertical to the bottom surface of the microwave sterilization box (9), and the bacterial colony suction pipe (507) is obliquely arranged on the mechanical disc (506) and is vertical to the mechanical disc (506); the rotation of the mechanical disk (506) can switch the inoculating loop (508) and the colony suction pipe (507).
In the embodiment, one side of the microwave sterilization box (9) is provided with a door body (6), and the door body is connected to the prism of the microwave sterilization box (9) through a hinge and is matched with the microwave sterilization box (9).
The utility model discloses a concrete operating procedure: placing the prepared culture medium (1) in the middle of a microwave sterilization box (9), dipping a small amount of bacteria-containing samples on an inoculating loop (508), closing a door (6), operating a mechanical arm, marking a plurality of continuous regular and non-repeated lines on a culture dish by the inoculating loop (508) at the tail end, standing for a period of time until bacterial colonies are formed, observing the formation condition of internal bacterial colonies through a touch screen (4), after the bacterial colonies are formed, transmitting information to a microcontroller (8) by a cell potential sensor (2), outputting a signal to a braking device (5) by the microcontroller (8), switching a mechanical disc (506) into a bacterial colony suction pipe (507), positioning the mechanical arm through position information given by the microcontroller (8), sucking pure bacterial colonies on the culture medium (1) by the bacterial colony suction pipe (507), opening the door (6), rotating a rotating shaft to convey the bacterial colony suction pipe (507) at the tail end of the mechanical arm to the outside of the microwave sterilization box, collect the pure kind bacterial colony in the bacterial colony straw, close door (6), open microwave sterilization case (9), kill all the bacterial colonies of inside abandonment, take out the culture medium again.
It is worth mentioning that: in the description of the present invention, "a plurality" means two or more unless specifically limited otherwise. In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; mechanical connection is possible, and a person of ordinary skill in the art can understand the specific meaning of the above terms in the present invention according to specific situations.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (5)
1. The utility model provides an automatic device that pure kind bacterial colony is selected in dull and stereotyped marking off which characterized in that: comprises a constant-temperature sterile microwave sterilization box, an automatic identification device which can comprehensively observe the bottom of the microwave sterilization box and a brake device which can finish bacterial colony transfer and plate lineation; the automatic identification device is positioned at the top of the microwave sterilization box, and the brake device is fixed on the inner side surface of the microwave sterilization box; the automatic identification device comprises an electron microscope positioned in the microwave sterilization box, a cell potential sensor at the tail end of the electron microscope and capable of identifying bacterial colonies, and a touch screen on the upper end surface of the microwave sterilization box, wherein the electron microscope is electrically connected with the touch screen; the braking device comprises a slide rail with one end fixed on the side surface of the microwave sterilization box and a mechanical arm capable of moving left and right on the slide rail, and the mechanical arm is slidably arranged on the slide rail; wherein,
the upper end surface of the microwave sterilization box is provided with a microcontroller, and the microcontroller is electrically connected with the mechanical arm and the automatic identification device;
a culture dish is placed at the bottom of the microwave sterilization box, and a solid culture medium with a flat plate line is filled in the culture dish.
2. The automated plate streaking apparatus for selecting purebred colonies as claimed in claim 1, wherein: the mechanical arm comprises a stepping motor, a rotating shaft, a first micro motor, a horizontal telescopic rod, a second micro motor, a vertical telescopic rod, a third micro motor and a mechanical disc, wherein the stepping motor is arranged on the sliding rail; the stepping motor is arranged on the slide rail and can move left and right, the rotating shaft is arranged above the stepping motor, the horizontal telescopic rod is arranged on the rotating shaft and is connected with the first micro motor, and the vertical telescopic rod is vertically arranged at the tail end of the horizontal telescopic rod and is connected with the second micro motor; the mechanical disc is obliquely arranged at the tail end of the vertical telescopic rod and is connected with one end of a third micro motor.
3. The automated plate streaking apparatus for selecting purebred colonies as claimed in claim 2, wherein: the bacterial colony suction pipe and the inoculating loop for plate scribing are installed on the mechanical disc, the inoculating loop is vertically installed on the mechanical disc and is perpendicular to the bottom surface of the microwave sterilization box, and the bacterial colony suction pipe is obliquely installed on the mechanical disc and is perpendicular to the mechanical disc.
4. The automated plate streaking apparatus for selecting purebred colonies as claimed in claim 1, wherein: the electron microscope comprises a lens cone, a spherical lens at the tail end of the lens cone and a support frame fixed on one side of the microwave sterilization box; the lens cone is fixed at the center of the microwave sterilization box by the other end of the support frame, and the spherical lens is detachably arranged at the tail end of the lens cone.
5. The automated plate streaking apparatus for selecting purebred colonies as claimed in claim 1, wherein: one side of the microwave sterilization box is provided with a door body, and the door body is connected to the prism of the microwave sterilization box through a hinge and is matched with the microwave sterilization box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920401835.6U CN209702757U (en) | 2019-03-26 | 2019-03-26 | A kind of plate streaking selects the automatic device of purebred bacterium colony |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920401835.6U CN209702757U (en) | 2019-03-26 | 2019-03-26 | A kind of plate streaking selects the automatic device of purebred bacterium colony |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209702757U true CN209702757U (en) | 2019-11-29 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920401835.6U Expired - Fee Related CN209702757U (en) | 2019-03-26 | 2019-03-26 | A kind of plate streaking selects the automatic device of purebred bacterium colony |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209702757U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350507A (en) * | 2022-03-17 | 2022-04-15 | 广东工业大学 | Single colony separation device and separation method for deep sea in-situ environment |
| CN116875423A (en) * | 2023-07-09 | 2023-10-13 | 硕邦(湖州)生物医药科技有限公司 | Preparation method of galactose yeast-like fermentation product composition |
-
2019
- 2019-03-26 CN CN201920401835.6U patent/CN209702757U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114350507A (en) * | 2022-03-17 | 2022-04-15 | 广东工业大学 | Single colony separation device and separation method for deep sea in-situ environment |
| WO2023173493A1 (en) * | 2022-03-17 | 2023-09-21 | 广东工业大学 | Single-colony isolation apparatus and isolation method for deep-sea in-situ environment |
| CN116875423A (en) * | 2023-07-09 | 2023-10-13 | 硕邦(湖州)生物医药科技有限公司 | Preparation method of galactose yeast-like fermentation product composition |
| CN116875423B (en) * | 2023-07-09 | 2024-03-19 | 硕邦(湖州)生物医药科技有限公司 | Preparation method of galactose yeast-like fermentation product composition |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191129 Termination date: 20210326 |
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| CF01 | Termination of patent right due to non-payment of annual fee |