CN206635346U - Fully-automatic intelligent cell culture apparatus - Google Patents
Fully-automatic intelligent cell culture apparatus Download PDFInfo
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- CN206635346U CN206635346U CN201620959406.7U CN201620959406U CN206635346U CN 206635346 U CN206635346 U CN 206635346U CN 201620959406 U CN201620959406 U CN 201620959406U CN 206635346 U CN206635346 U CN 206635346U
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Abstract
A kind of fully-automatic intelligent cell culture apparatus is the utility model is related to, it includes controller, main body（5）, the first governor motion, the second governor motion, the 3rd governor motion, the 4th governor motion, culturing rack（6）, first control door（7）, second control door（8）, the first pipetting device, the second pipetting device, waste collection mechanism（9）, switch bottle mechanism（10）, filling mechanism（11）And culture medium tank.Change that liquid is convenient, fast, accurate and cell growth does not influence using this culture apparatus.
Description
Technical field
It the utility model is related to technical field of cell culture, more particularly to a kind of fully-automatic intelligent cell culture apparatus.
Background technology
In RESEARCH ON CELL-BIOLOGY field and life science field, cell culture is most basic link.Cell
Culture is cell under artificial environment, using nutriment etc., is bred increment, and for the culture of " various purposes "
Journey.
Prior art cell culture needs to be divided to two environment, and one is the nutrient solution storage environment in low temperature, and one is
Cell culture environment in preference temperature, what prior art was both provided separately, when needing to carry out culture fluid exchange,
Need to take out the culture jar for depositing nutrient solution in the manual culture environment therefrom of operating personnel, from another culture environment
Middle taking-up Tissue Culture Flask, then worker carries out the replacing of nutrient solution by instrument again, finally again respectively by culture jar and
Tissue Culture Flask is put back into respective culture environment, and such mode of operation has the disadvantages that：
First, using manually operated, it is difficult to the dosage of accurate control nutrient solution；
2nd, it is manually operated to need to take out Tissue Culture Flask into operation from culture environment, the growth of cell may be influenceed,
Certain influence is produced on the accuracy of experiment；
3rd, using manually operated, so culture fluid exchange is less efficient, and easily malfunction；
4th, using manually operated, it is impossible to which the arbitrarily selected time for changing nutrient solution when having operator, it is necessary to can just carry out
Change.
Utility model content
Technical problem to be solved in the utility model is：One kind is provided and changes that liquid is convenient, fast, accurate and cell growth
Do not have influential fully-automatic intelligent cell culture apparatus.
Technical scheme is used by the utility model：A kind of fully-automatic intelligent cell culture apparatus, it is characterised in that：It
Including controller, at least provided with storage chamber, culture chamber, operating cavity and observe four chambers of chamber main body, be arranged on storage chamber
It is interior to be used to adjust the first governor motion of storage chamber ambient condition, be arranged in culture chamber for adjusting culture chamber ambient condition
Second governor motion, it is arranged in operating cavity for adjusting the 3rd governor motion of operating cavity ambient condition, being arranged on observation chamber
It is interior to be used to adjust the 4th governor motion of observation chamber ambient condition, be arranged on the culture for placing Tissue Culture Flask in culture chamber
Frame, be arranged on culture chamber and observe chamber between first control door, be arranged on observation chamber and operating cavity between second control door,
It is arranged on and is used to pick and place Tissue Culture Flask in culture chamber and drives Tissue Culture Flask to be moved to observation chamber is observed first
Pipetting device, it is arranged on the Tissue Culture Flask brought in operating cavity for the first pipetting device of crawl and controls blake bottle to carry out
Second pipetting device of action, the waste collection for being used to collect the waste liquid poured out from Tissue Culture Flask being arranged in operating cavity
Mechanism, it is arranged in operating cavity for switching the switch bottle mechanism of cell culture bottle cap, being arranged in operating cavity for thin
The filling mechanism of born of the same parents' blake bottle liquid feeding and be arranged on and be used for the culture medium tank for storing culture medium in storage chamber, it is described plus
Liquid mechanism is connected with culture medium tank by conduit, and it is described first control door, second control door, the first pipetting device,
Second pipetting device, waste collection mechanism, switch bottle mechanism, filling mechanism, the first governor motion, the second governor motion, the 3rd
Governor motion and the 4th governor motion are electrically connected with the controller.
First pipetting device include be arranged in culture chamber the first track, be arranged on observation intracavitary and with the first rail
Second track of road connection, it is slidably connected at the first track and first slide on the second track and is arranged in first slide
For the first manipulator of pick-and-place Tissue Culture Flask, the first slide and the first manipulator are electrically connected with the controller；Institute
Stating the second pipetting device includes being arranged on the 3rd track being connected in operating cavity and with the second track, is slidably connected at the 3rd track
On second slide and being arranged in second slide be used for pick-and-place Tissue Culture Flask and dynamic for driving Tissue Culture Flask to perform
The second manipulator made, the second slide and the second manipulator are electrically connected with the controller.
The Tissue Culture Flask be provided with for crawl fixing clip, first manipulator and the second manipulator with
Fixed block matches.
The observation intracavitary is provided with the transparent mounting table placed for Tissue Culture Flask and is arranged under transparent mounting table
In the observation imaging mechanism of observation Tissue Culture Flask inner cell growing state, the observation imaging mechanism is electrically connected with the controller.
The storage chamber is divided into three chambers, and culture medium tank, buffer solution storage are respectively equipped with three chambers
Container and digestive juice tank, the culture medium tank, buffer solution tank and digestive juice tank are equal
Connected by conduit with filling mechanism, and adding for the outflow of culture medium, buffer solution and digestive juice is respectively equipped with filling mechanism
Liquid mouth.
The filling mechanism is provided with the culture medium preheating mechanism for being used for preheating culture medium, and the culture medium preheating mechanism
It is electrically connected with the controller.
The first rack placed for Tissue Culture Flask midway, the cell training for storing sky are additionally provided with the operating cavity
Support the second rack of bottle, the bottle cap switching mechanism for switching empty cell culture bottle cap, for placing the useless of discarded bottle
Bottle rack, the suction pipe holder for placing suction pipe and the suction pipe disposable box for collecting used suction pipe, and described the
It is additionally provided with two manipulators and coordinates pressure-vaccum mechanism for pressure-vaccum suction pipe with suction pipe, and the pressure-vaccum mechanism is electrically connected with controller
Connect.
First governor motion includes the first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor
And first humidity sensor, second adjusting means include second temperature governor motion, the second humidity regulation mechanism, dioxy
Change concentration of carbon governor motion, second temperature sensor, the second humidity sensor and gas concentration lwevel sensor, the described 3rd
Governor motion includes the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, three-temperature sensor and the 3rd humidity sensor
Device, the 4th governor motion include the 4th thermoregulation mechanism, the 4th humidity regulation mechanism, the 4th temperature sensor and the
Four humidity sensors, first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor, the first humidity pass
Sensor, second temperature governor motion, the second humidity regulation mechanism, gas concentration lwevel governor motion, second temperature sensor,
Two humidity sensors, gas concentration lwevel sensor, the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, the 3rd temperature pass
Sensor, the 3rd humidity sensor, the 4th thermoregulation mechanism, the 4th humidity regulation mechanism, the 4th temperature sensor and the 4th
Humidity sensor is electrically connected with the controller.
Using above mechanism compared with prior art, the utility model has advantages below：Pass through controller control machinery
Hand is completed to change liquid operation, so there is no need to manually operated, can accurately control the dosage of nutrient solution；And cell need not be trained
Foster bottle takes out from culture environment changes liquid again, so the growth of cell will not be higher by too big influence, test accuracy；And
Carry out changing liquid using controller control, error rate is relatively low；As long as and set and place enough culture mediums and can be achieved
Automatically change liquid, it is not necessary to which operator is on the scene in real time, and to be observed there is provided chamber is observed before and after liquid is changed, and so can guarantee that
The accuracy of experiment is higher.
Liquid is changed to realize using the combination of track, slide and manipulator, it is so relatively simple for structure, and change liquor ratio compared with
It is convenient.
And it will largely change liquid operation to be arranged in operating cavity, do not interfere with the cell cultivated in culture chamber so
Blake bottle so that test accuracy is more preferable.
Fixed block is set on Tissue Culture Flask, manipulator can be made preferably to capture Tissue Culture Flask.
And buffer solution and digestive juice are also provided with storage chamber, and also set up in operating cavity the first rack,
Second rack, bottle cap switching mechanism, useless bottle rack, suction pipe holder and suction pipe disposable box, and in the second manipulator
It is upper to be provided with pressure-vaccum mechanism, so that device has had digestive transfer culture function in itself so that allomeric function is more diversified.
Preheating mechanism is provided with filling mechanism, such culture medium can first carry out pre- before adding in Tissue Culture Flask
Heat so that experiment is more accurate.
Set using the first governor motion of these modules, the second governor motion, the 3rd governor motion and the 4th regulation
Mechanism, it can preferably keep the environment with regulation culture chamber, observation chamber, operating cavity and storage chamber.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model fully-automatic intelligent cell culture apparatus.
As shown in the figure：1st, storage chamber；2nd, culture chamber；3rd, operating cavity；4th, chamber is observed；5th, main body；6th, culturing rack；7th, the first control
Door processed；8th, the second control door；9th, waste collection mechanism；10th, bottle mechanism is switched；11st, filling mechanism；12nd, the first track；13rd,
Two tracks；14th, first slide；15th, the first manipulator；16th, the 3rd track；17th, second slide；18th, the second manipulator；19th, thoroughly
Bright mounting table；20th, imaging mechanism is observed；21st, the first rack；22nd, the second rack.
Embodiment
The utility model is described further with embodiment below in conjunction with accompanying drawing, but the utility model is not only
It is limited to detailed description below.
A kind of fully-automatic intelligent cell culture apparatus, it includes controller, at least provided with storage chamber 1, culture chamber 2, operation
The main body 5 of chamber 3 and observation 4 four chambers of chamber, it is arranged on the first regulation for adjusting storage chamber ambient condition in storage chamber 1
Mechanism, it is arranged in culture chamber 2 and is used to adjusting the second governor motion of culture chamber ambient condition, is arranged in operating cavity 3 and is used for
Adjust the 3rd governor motion of operating cavity ambient condition, be arranged in observation chamber 4 for adjusting the 4th of observation chamber ambient condition
Governor motion, be arranged in culture chamber 2 be used to placing the culturing rack 6 of Tissue Culture Flask, be arranged on culture chamber 2 and observation chamber 4 it
Between the first control door 7, be arranged between observation chamber 4 and operating cavity 3 the second control door 8, be arranged in culture chamber 2 and be used to take
Put Tissue Culture Flask and drive Tissue Culture Flask to be moved to the first pipetting device observed chamber and observed, be arranged on operating cavity
3 interior the second pipetting devices for being used to capture the Tissue Culture Flask that the first pipetting device is brought and control blake bottle to be acted,
It is arranged on being used to collect the waste collection mechanism 9 for the waste liquid poured out from Tissue Culture Flask, being arranged on operating cavity in operating cavity 3
It is used to switch the switch bottle mechanism 10 of cell culture bottle cap in 3, is arranged in operating cavity 3 for Tissue Culture Flask liquid feeding
Filling mechanism 11 and be arranged on and be used for the culture medium tank for storing culture medium in storage chamber 1, the filling mechanism 11 with
Culture medium tank is connected by conduit, and the first control door 7, second controls door 8, the first pipetting device, second to move
Liquid mechanism, waste collection mechanism 9, switch bottle mechanism 10, filling mechanism 11, the first governor motion, the second governor motion, the 3rd adjust
Section mechanism and the 4th governor motion are electrically connected with the controller.Waste collection mechanism 9 can be a container, switch bottle mechanism
10 include a chuck and for driving the first elastic actuating unit of chuck and for driving the second of chuck rotation to move
Force mechanisms, filling mechanism can use a pump, after unlatching by by conduit by the liquid in tank drink up then from
Filling opening flows out.And the storage chamber 1, culture chamber 2, operating cavity 3 and observation 4 four chambers of chamber are equipped with disinfecting mechanism, this
Sample needs to open four before on-test before will being put into culture chamber equipped with the Tissue Culture Flask of cell and culture medium
Disinfecting mechanism carries out pasteurised completely, after experiment, i.e., after the debris such as all Tissue Culture Flasks and waste liquid in main body is taken out,
Need to open four disinfecting mechanisms progress pasteurised completelies.
The first track 12 that first pipetting device includes being arranged in culture chamber 2, it is arranged in observation chamber 4 and with the
Second track 13 of one track 12 connection, it is slidably connected at the first track 12 and first slide 14 on the second track 13 and sets
Put the first manipulator 15 for being used for pick-and-place Tissue Culture Flask in first slide 14, the manipulator of first slide 14 and first
15 are electrically connected with the controller；Second pipetting device includes being arranged on the be connected in operating cavity 3 and with the second track 13
Three tracks 16, the second slide 17 being slidably connected on the 3rd track 16 and it is arranged in second slide 17 and is used for pick-and-place cell
Blake bottle and the second manipulator 18 for driving Tissue Culture Flask execution to act, the manipulator of second slide 17 and second
18 are electrically connected with the controller.Track in accompanying drawing does not represent complete.
The Tissue Culture Flask is provided with the fixing clip for crawl, the manipulator 18 of the first manipulator 15 and second
Match with fixed block.
The transparent mounting table 19 placed for Tissue Culture Flask is provided with the observation chamber 4 and is arranged on transparent mounting table 19
The lower observation imaging mechanism 20 for being used to observe Tissue Culture Flask inner cell growing state, the observation imaging mechanism 20 and controller
Electrical connection.
The storage chamber 1 is divided for three chambers, and culture medium tank, buffer solution storage are respectively equipped with three chambers
Container and digestive juice tank, the culture medium tank, buffer solution tank and digestive juice tank are equal
Connected by conduit with filling mechanism 11, and be respectively equipped with filling mechanism 11 and flowed out for culture medium, buffer solution and digestive juice
Filling opening.
The filling mechanism 11 is provided with the culture medium preheating mechanism for being used for preheating culture medium, and the culture medium preheating machine
Structure is electrically connected with the controller.An accommodating cavity for being used to house culture medium is provided with filling mechanism 11, preheating mechanism is arranged on appearance
Put intracavitary.
The first rack 21 for the placement of Tissue Culture Flask midway is additionally provided with the operating cavity 3, for storing the thin of sky
The bottle cap switching mechanism, discarded for placing of second rack 22 of born of the same parents' blake bottle, cell culture bottle cap for switching sky
Useless bottle rack, the suction pipe holder for placing suction pipe and the suction pipe disposable box for collecting used suction pipe of bottle, and
It is additionally provided with second manipulator 18 and coordinates pressure-vaccum mechanism for pressure-vaccum suction pipe, and the pressure-vaccum mechanism and control with suction pipe
Device electrically connects.These mechanisms are for realizing had digestive transfer culture and increased, if simply liquid is changed in realization, do not need these machines
Structure, so not showing in the accompanying drawings.And together with bottle cap switching mechanism can do with switch bottle mechanism 10, useless bottle is put
Put together with frame can do with waste collection mechanism 9.
First governor motion includes the first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor
And first humidity sensor, second adjusting means include second temperature governor motion, the second humidity regulation mechanism, dioxy
Change concentration of carbon governor motion, second temperature sensor, the second humidity sensor and gas concentration lwevel sensor, the described 3rd
Governor motion includes the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, three-temperature sensor and the 3rd humidity sensor
Device, the 4th governor motion include the 4th thermoregulation mechanism, the 4th humidity regulation mechanism, the 4th temperature sensor and the
Four humidity sensors, first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor, the first humidity pass
Sensor, second temperature governor motion, the second humidity regulation mechanism, gas concentration lwevel governor motion, second temperature sensor,
Two humidity sensors, gas concentration lwevel sensor, the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, the 3rd temperature pass
Sensor, the 3rd humidity sensor, the 4th thermoregulation mechanism, the 4th humidity regulation mechanism, the 4th temperature sensor and the 4th
Humidity sensor is electrically connected with the controller.
A kind of control method of fully-automatic intelligent cell culture apparatus, it includes changing liquid method and had digestive transfer culture method,
The liquid method of changing comprises the following steps：
（1）, controller control the first manipulator crawl Tissue Culture Flask, then control first control door open, then control
First slide processed, which is slided, drives the first manipulator to extend into observation intracavitary, and Tissue Culture Flask is placed on transparent mounting table afterwards
Observation, simultaneously closes off the first control door；
（2）, controller control the crawl of the first manipulator to be placed on Tissue Culture Flask on transparent mounting table, then control the
Two control doors are opened, and then control first slide movement to drive the first manipulator to enter operating cavity；
（3）, controller control second slide movement drive the second manipulator to be moved at the first manipulator, then control the
Two manipulators capture the Tissue Culture Flask on the first manipulator, while control the first manipulator to unclamp Tissue Culture Flask, same to time control
The control door of system second is closed；
（4）, controller control second slide movement by the second manipulator with Tissue Culture Flask be moved to switch bottle machine
At structure, then controlling switch bottle mechanism backs out the bottle cap of Tissue Culture Flask；
（5）, controller control second slide movement the second manipulator with Tissue Culture Flask is moved to waste collection
At mechanism, then control the second manipulator to rotate and then the waste liquid in Tissue Culture Flask is poured into waste collection mechanism；
（6）, controller control second slide movement the second manipulator with Tissue Culture Flask is moved to filling mechanism
Place, while control culture medium preheating mechanism to preheat the culture medium in filling mechanism, filling mechanism is then controlled to cell culture
The culture medium extracted from culture medium tank is added in bottle；
（7）, controller control second slide movement by the second manipulator with Tissue Culture Flask be moved to switch bottle machine
At structure, then controlling switch bottle mechanism screws back bottle cap on Tissue Culture Flask again；
（8）, controller control second slide movement by the second manipulator with Tissue Culture Flask be moved to first machinery
At hand, the first manipulator is then controlled to capture Tissue Culture Flask, while control the second manipulator to unclamp Tissue Culture Flask；
（9）, controller control second control door open, then control first slide drive the first manipulator enter observation
Chamber, then control the first manipulator that Tissue Culture Flask is placed on transparent mounting table and observe, simultaneously close off the second control door；
（10）, controller control first control door open, then control the first manipulator crawl be placed on transparent mounting table
On Tissue Culture Flask, then control first slide movement to drive the first manipulator to enter culture chamber, it is first mechanical to control afterwards
Tissue Culture Flask is placed on the culturing rack originally taken out by hand, simultaneously closes off the first control door.
The had digestive transfer culture method comprises the following steps：
A, controller controls the first manipulator crawl Tissue Culture Flask, then controls the first manipulator to enter observation chamber and sees
Survey, then control the first manipulator to enter operating cavity, control the second manipulator to capture cell culture at the first manipulator afterwards
Bottle；
B, controller controls the second manipulator to be moved at switch bottle mechanism and opens bottle cap, then move to waste collection machine
Waste liquid is poured out at structure, filling mechanism is then moved to and adds buffer solution, is moved to afterwards at switch bottle mechanism and shuts bottle cap；
C, controller controls the second manipulator to rock Tissue Culture Flask, makes buffer solution and the cell in Tissue Culture Flask abundant
Contact；
D, controller controls the second manipulator to be moved at switch bottle mechanism and opens bottle cap, then move to waste collection machine
Waste liquid is poured out at structure, filling mechanism is then moved to and adds digestive juice；
E, controller controls the second manipulator that Tissue Culture Flask is placed at the first rack, then controls the second machinery
Hand captures suction pipe out of suction pipe holder, then extend into suction pipe in the Tissue Culture Flask being placed at the first rack and passes through
Pressure-vaccum mechanism is blown and beaten；
F, controller controls the second manipulator that suction pipe is put into suction pipe disposable box, then controls the second manipulator will be from the
Tissue Culture Flask is captured on one rack, then moves at switch bottle mechanism and shuts bottle cap, the first manipulator is from second afterwards
Tissue Culture Flask is captured at manipulator, and Tissue Culture Flask is sent into observation intracavitary observation, until observing Tissue Culture Flask
It is digested to finish, enter back into next step；
G controllers control the first manipulator crawl Tissue Culture Flask to enter operating cavity, then control the second manipulator to capture
Tissue Culture Flask, it is moved at switch bottle mechanism and opens bottle cap, be moved to the training that addition had been warmed up at filling mechanism afterwards
Support base and terminate digestion；
H, controller controls the second manipulator to be moved to the culture medium that addition is preheated at filling mechanism, then will add
The Tissue Culture Flask of culture medium is placed on the first rack；
I, controller controls the crawl of the second manipulator to be placed on the empty Tissue Culture Flask on the second rack, then moves
Bottle cap is opened at bottle cap switching mechanism, then moves at the second rack and the Tissue Culture Flask of sky is placed on the second placement
On frame；
J, controller controls the crawl of the second manipulator to be placed on the suction pipe in suction pipe holder, then stretches into suction pipe and is equipped with
The Tissue Culture Flask of culture medium and cell, then control pressure-vaccum mechanism to draw a part of cell and culture medium, will draw afterwards
To cell and culture medium be put into by suction pipe in the empty Tissue Culture Flask being placed on the second rack；
L, controller controls the crawl of the second manipulator to be placed on the blake bottle on the second rack, then moves to bottle cap and opens
Shutting mechanism shuts bottle cap, and then the first manipulator captures this Tissue Culture Flask, is moved to observation intracavitary observation, is sent into training afterwards
On the culturing rack for supporting intracavitary；
L, circulation step I to step L successively, until the cell in original Tissue Culture Flask and culture medium by
After being assigned in the Tissue Culture Flask of sky, and newly the Tissue Culture Flask of addition cell and culture medium has been placed within
After culturing rack in culture chamber, the Tissue Culture Flask for having taken cell and culture medium is placed into by the second manipulator of control
Give up bottle rack, then end operation.
Above-mentioned steps are that the cell dissociation passage in a Tissue Culture Flask has been arrived into multiple blake bottles, if desired will
Equal had digestive transfer culture in Tissue Culture Flask on all original culturing racks, then circulation step A is needed to step M, until all
The equal had digestive transfer culture of Tissue Culture Flask finishes.
And the first manipulator is moved to particular location is in the first track and second by first slide in above-mentioned steps
Slide what is realized on track, and the first manipulator needs first to open the first control door from culture chamber to observation chamber, from sight
Survey chamber is needed first to open the second control door to operating cavity, and the second manipulator, which is moved to particular location, to be existed by second slide
Slide what is realized on 3rd track, and the first manipulator can carry out pick-and-place operation and the movement of limited range, so that
First robot manipulator structure can be with fairly simple, and the second manipulator can not only realize pick-and-place operation and the movement of limited range, and
And reversing can also be realized and rock operation.
Claims (8)
- A kind of 1. fully-automatic intelligent cell culture apparatus, it is characterised in that：It includes controller, at least provided with storage chamber (1), training Support chamber (2), operating cavity (3) and observe the main body (5) of (4) four chambers of chamber, be arranged in storage chamber (1) for adjusting storage First governor motion of chamber ambient condition, it is arranged on the second regulation machine for adjusting culture chamber ambient condition in culture chamber (2) Structure, it is arranged in operating cavity (3) for adjusting the 3rd governor motion of operating cavity ambient condition, being arranged in observation chamber (4) and use It is used for the culture for placing Tissue Culture Flask in culture chamber (2) in adjusting the 4th governor motion of observation chamber ambient condition, being arranged on Frame (6), the first control door (7) for being arranged on culture chamber (2) and observing between chamber (4), it is arranged on observation chamber (4) and operating cavity (3) second between controls door (8), is arranged in culture chamber (2) for picking and placeing Tissue Culture Flask and driving Tissue Culture Flask The first pipetting device that observation chamber observed is moved to, is arranged in operating cavity (3) to be used to capturing the first pipetting device and passes through The Tissue Culture Flask that comes simultaneously controls the second pipetting device that blake bottle acted, is arranged in operating cavity (3) and is used to collect The waste collection mechanism (9) for the waste liquid poured out from Tissue Culture Flask, it is arranged in operating cavity (3) for switching Tissue Culture Flask The switch bottle mechanism (10) of bottle cap, be arranged on be used in operating cavity (3) to Tissue Culture Flask liquid feeding filling mechanism (11) and It is arranged on and is used for the culture medium tank for storing culture medium in storage chamber (1), the filling mechanism (11) is stored with culture medium Container is connected by conduit, and it is described first control door (7), second control door (8), the first pipetting device, the second pipetting device, Waste collection mechanism (9), switch bottle mechanism (10), filling mechanism (11), the first governor motion, the second governor motion, the 3rd adjust Section mechanism and the 4th governor motion are electrically connected with the controller.
- 2. fully-automatic intelligent cell culture apparatus according to claim 1, it is characterised in that：The first pipetting device bag Include the first track (12) being arranged in culture chamber (2), be arranged on second be connected in observation chamber (4) and with the first track (12) Track (13), the first slide (14) being slidably connected on the first track (12) and the second track (13) and it is arranged on the first cunning It is used for the first manipulator (15) of pick-and-place Tissue Culture Flask, the first slide (14) and the first manipulator (15) on seat (14) It is electrically connected with the controller；Second pipetting device includes being arranged on what is be connected in operating cavity (3) and with the second track (13) 3rd track (16), the second slide (17) being slidably connected on the 3rd track (16) and being arranged in second slide (17) is used In pick-and-place Tissue Culture Flask and the second manipulator (18) for driving Tissue Culture Flask execution to act, the second slide (17) And second manipulator (18) be electrically connected with the controller.
- 3. fully-automatic intelligent cell culture apparatus according to claim 2, it is characterised in that：Set on the Tissue Culture Flask There is the fixing clip for capturing, first manipulator (15) and the second manipulator (18) match with fixed block.
- 4. fully-automatic intelligent cell culture apparatus according to claim 1, it is characterised in that：Set in the observation chamber (4) There is the transparent mounting table (19) placed for Tissue Culture Flask and be arranged under transparent mounting table (19) and be used to observe cell culture The observation imaging mechanism (20) of bottle inner cell growing state, the observation imaging mechanism (20) are electrically connected with the controller.
- 5. fully-automatic intelligent cell culture apparatus according to claim 1, it is characterised in that：The storage chamber (1) is divided into Three chambers, and culture medium tank, buffer solution tank and digestive juice tank are respectively equipped with three chambers, The culture medium tank, buffer solution tank and digestive juice tank are connected by conduit and filling mechanism (11) It is logical, and the filling opening for the outflow of culture medium, buffer solution and digestive juice is respectively equipped with filling mechanism (11).
- 6. fully-automatic intelligent cell culture apparatus according to claim 1, it is characterised in that：On the filling mechanism (11) Provided with the culture medium preheating mechanism for preheating culture medium, and the culture medium preheating mechanism is electrically connected with the controller.
- 7. fully-automatic intelligent cell culture apparatus according to claim 2, it is characterised in that：In the operating cavity (3) also Second rack of the Tissue Culture Flask provided with the first rack (21) placed for Tissue Culture Flask midway, for storing sky (22), for switching the bottle cap switching mechanism of cell culture bottle cap of sky, the useless bottle rack for placing discarded bottle, being used for The suction pipe holder of suction pipe and the suction pipe disposable box for collecting used suction pipe are placed, and on second manipulator (18) It is additionally provided with and coordinates pressure-vaccum mechanism for pressure-vaccum suction pipe with suction pipe, and the pressure-vaccum mechanism is electrically connected with the controller.
- 8. fully-automatic intelligent cell culture apparatus according to claim 1, it is characterised in that：The first governor motion bag Include the first thermoregulation mechanism, the first humidity regulation mechanism, the first temperature sensor and the first humidity sensor, described second Adjusting means includes second temperature governor motion, the second humidity regulation mechanism, gas concentration lwevel governor motion, second temperature and passed Sensor, the second humidity sensor and gas concentration lwevel sensor, the 3rd governor motion include the 3rd temperature adjuster Structure, the 3rd humidity regulation mechanism, three-temperature sensor and the 3rd humidity sensor, the 4th governor motion include the 4th Thermoregulation mechanism, the 4th humidity regulation mechanism, the 4th temperature sensor and the 4th humidity sensor, first temperature are adjusted Save mechanism, the first humidity regulation mechanism, the first temperature sensor, the first humidity sensor, second temperature governor motion, second wet Governor motion, gas concentration lwevel governor motion, second temperature sensor, the second humidity sensor, gas concentration lwevel is spent to pass Sensor, the 3rd thermoregulation mechanism, the 3rd humidity regulation mechanism, three-temperature sensor, the 3rd humidity sensor, the 4th temperature Governor motion, the 4th humidity regulation mechanism, the 4th temperature sensor and the 4th humidity sensor are electrically connected with the controller.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620959406.7U CN206635346U (en) | 2016-08-29 | 2016-08-29 | Fully-automatic intelligent cell culture apparatus |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620959406.7U CN206635346U (en) | 2016-08-29 | 2016-08-29 | Fully-automatic intelligent cell culture apparatus |
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| CN206635346U true CN206635346U (en) | 2017-11-14 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244455A (en) * | 2016-08-29 | 2016-12-21 | 宁波键生物科技有限公司 | Fully-automatic intelligent cell culture system and control method thereof |
| WO2021120238A1 (en) * | 2019-12-20 | 2021-06-24 | 李晓 | Automatic testing equipment for microorganisms |
| CN117229918A (en) * | 2023-11-16 | 2023-12-15 | 四川迪亚生物科技集团有限公司 | Pump-free driving liquid pouring device and method |
-
2016
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| CN106244455A (en) * | 2016-08-29 | 2016-12-21 | 宁波键生物科技有限公司 | Fully-automatic intelligent cell culture system and control method thereof |
| CN106244455B (en) * | 2016-08-29 | 2018-09-21 | 杭州键一生物科技有限公司 | Fully-automatic intelligent cell culture apparatus and its control method |
| WO2021120238A1 (en) * | 2019-12-20 | 2021-06-24 | 李晓 | Automatic testing equipment for microorganisms |
| CN117229918A (en) * | 2023-11-16 | 2023-12-15 | 四川迪亚生物科技集团有限公司 | Pump-free driving liquid pouring device and method |
| CN117229918B (en) * | 2023-11-16 | 2024-01-30 | 四川迪亚生物科技集团有限公司 | Pump-free driving liquid pouring device and method |
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Address after: 311225 Jiangdong industrial cluster area, Hangzhou, Zhejiang Province, No. 616, Jiangdong two road Pioneer Park, D Patentee after: Hangzhou key biological technology Co., Ltd. Address before: 315174 Yinzhou District, Zhejiang, Xueyuan Road, No. 899, building, information technology, layer 4 Patentee before: NINGBO JIANYI BIOTECHNOLOGY CO., LTD. |