CN110129200A - A kind of 3D stem cell emulation culture device - Google Patents
A kind of 3D stem cell emulation culture device Download PDFInfo
- Publication number
- CN110129200A CN110129200A CN201910408089.8A CN201910408089A CN110129200A CN 110129200 A CN110129200 A CN 110129200A CN 201910408089 A CN201910408089 A CN 201910408089A CN 110129200 A CN110129200 A CN 110129200A
- Authority
- CN
- China
- Prior art keywords
- valve
- air pump
- liquid
- cell
- material bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2513/00—3D culture
Abstract
A kind of 3D stem cell emulation culture device, it is related to biomedical technical field of cell culture, and in particular to a kind of stem cell culture device.The invention aims to solve cell culture apparatus at this stage to control the environment of cell culture at any time, carrying out cell separation by hand, not only process is cumbersome, and it is readily incorporated pollution, for artificial liquid relief behaviour's overlong time since fatigue causes operating error, there is error to count in people.A kind of 3D stem cell emulation culture device includes cell culture and isolated device, liquid shifting equipment and counting equipment；The invention has the advantages that integrating cell culture, cell separation, cell count process etc., the efficiency of cell culture is greatly improved, makes cell culture more safely, conveniently.Reduce pipetting processes manual operation bring error.Avoid the error in reading and evaluated error of manual operation.Present invention is mainly used for cell culture, cell separation and cell counts.
Description
Technical field
The present invention relates to biomedical technical field of cell culture, and in particular to a kind of stem cell culture device.
Background technique
Cell culture refer to environment Imitating vivo environment culture in vitro take out cell in vivo and make cells survival and
The technology of growth.From 1855 till now, the research of cell culture has developed into the research hotspot of field of biomedicine, carefully
Born of the same parents' culture also becomes widely used technological means in biomedical research application.
However, most of cell culture at present is all using the method manually cultivated.Due to the precision of manual operation is insufficient,
The inferior numerous reasons of low efficiency, there are many deficiencies for manually-operated method, it is difficult to reach operational normative standard, also very
It is easy to cause the problems such as culture efficiency is low, and safety is poor.Cell culture apparatus at this stage cannot control cell at any time mostly
The environment of culture, this be easy for causing happening suddenly in cell cultivation process it is expected except the case where and it is uncontrollable.
It after cell culture is completed, is typically necessary and cell is separated, currently, many cell separation are all manpower works
It carries out, it is cumbersome to carry out not only process by hand, but also be readily incorporated pollution, influence cell activity.
After cell separation, researcher is generally required to cell count.It then needs to carry out liquid relief behaviour before counting operation
Make, the key of liquid relief is to realize the acquisition and transfer of liquid accurately and fast.Artificial pipetting needs staff's high concentration
Attention, this is just easy to because overlong time is since fatigue causes operating error.Traditional cell count mode is that employment comes
It counts, people, which counts general, can count regional area in cell counting board to estimate whole cell number, just there is people among these
The error of counting and the error of estimation.
Summary of the invention
The invention aims to solve cell culture apparatus at this stage to control the environment of cell culture, hand at any time
Work carries out cell separation, and not only process is cumbersome, but also is readily incorporated pollution, and artificial liquid relief behaviour's overlong time causes to grasp due to fatigue
Make error, people has error to count, and provides a kind of 3D stem cell emulation culture device.
A kind of 3D stem cell emulation culture device, it includes that cell culture and isolated device, liquid shifting equipment and counting are set
It is standby；
The cell culture and isolated device include the first lid, the second lid, the first pressure regulator valve, Tissue Culture Flask, capacity
Bottle, the first peristaltic pump, dialysis liquid material bottle, the first pump, waste material bottle, dialyzer, the second pressure regulator valve, the 6th check valve, the 5th air pump,
7th check valve, the 6th air pump, waste liquid support base-material bottle, the second peristaltic pump, the second pump, gas-exchange membrane, third valve, continous way from
Scheming, the 4th valve, the 6th valve, colloidal sol waste liquid material bottle, cleaning waste liquid calcium wasting liquid material bottle, the 5th valve, the 5th check valve, the 8th gas
Pump, the 7th air pump, the first triple valve and the second triple valve；
The inoculating cell stage: Tissue Culture Flask and the first lid connect, and the first lid includes the first air pump, the first check valve, perseverance
Stream pump, the headkerchief with funnel, the first valve, second one-way valve and the second air pump, the headkerchief with funnel cover on Tissue Culture Flask, band
The headkerchief of funnel is connect with the first air pump, constant flow pump and the second air pump, is arranged first between the first air pump and headkerchief with funnel
Second one-way valve is set between check valve, the second air pump and headkerchief with funnel, is connected the lid of Tissue Culture Flask by tee tube
It connects cleaning waste liquid calcium wasting liquid material bottle and waste liquid supports base-material bottle, the first valve is set on the inlet pipeline of tee tube；
Cultivate cell stage: Tissue Culture Flask is connected with the second lid, and the second lid includes third air pump, third check valve, no
Headkerchief with funnel, the second valve, the 4th check valve and the 4th air pump；It is covered on Tissue Culture Flask without the headkerchief of funnel, without
The headkerchief of funnel is connect with third air pump, the 4th air pump, continuous centrifugal machine and volumetric flask, third air pump and the lid without funnel
Head between be arranged third check valve, the 4th air pump and without between the headkerchief of funnel be arranged the 4th check valve, volumetric flask with without
First pressure regulator valve is set between the headkerchief of funnel, continuous centrifugal machine and without the second valve is arranged between the headkerchief of funnel, continuously
Formula centrifuge and without setting gradually the 5th valve and the 8th air pump in the connecting pipe between the headkerchief of funnel, Tissue Culture Flask
Bottom connects the 7th air pump, gas-exchange membrane, cleaning waste liquid calcium wasting liquid material bottle and waste liquid and supports base-material bottle, and the 7th air pump and cell are trained
It supports and the 5th check valve is set between bottle, third valve is set between gas-exchange membrane and Tissue Culture Flask, it will by the second triple valve
Tissue Culture Flask is supported base-material bottle with cleaning waste liquid calcium wasting liquid material bottle and waste liquid and is connect, continuous centrifugal machine and the headkerchief without funnel
Between connecting pipe be connected to the connecting pipe between the second triple valve and Tissue Culture Flask by the 4th valve, continuous centrifugal
The waste liquid port of machine is connected to colloidal sol waste liquid material bottle, and the 6th valve, volumetric flask are arranged between colloidal sol waste liquid material bottle and continuous centrifugal machine
Top connect the first peristaltic pump, volumetric flask connect with gas-exchange membrane, and setting second is compacted between volumetric flask and gas-exchange membrane
Dynamic pump, volumetric flask are connect with dialyzer, and the second pressure regulator valve, volumetric flask and the 5th air pump and the are arranged between volumetric flask and dialyzer
The connection of six air pumps, is arranged the 6th check valve between volumetric flask and the 6th air pump, it is single that the 7th is arranged between volumetric flask and the 6th air pump
To valve, dialyzer is supported with the first peristaltic pump and waste liquid by base-material bottle by the first triple valve and is connect, is pumped by first by dialyzer
It is connect with dialysis liquid material bottle, the waste outlet of dialyzer is connected to waste material bottle；Gas-exchange membrane and the second pump connect；
The liquid shifting equipment include six degree of freedom robotic arm, liquid-transfering gun control instrument hand, liquid-transfering gun, stem cell charging box and
Centrifuge tube；Liquid-transfering gun control instrument hand is fixed on six degree of freedom robotic arm, and liquid-transfering gun is fixed on liquid-transfering gun control instrument on hand,
Centrifuge tube loads in stem cell charging box；
The counting equipment includes moveable stage, cell counting board, microscope, industrial camera, high-performance computer
And stepper motor；Moveable stage is set on microscope, and moveable stage and stepper motor are electrically connected, and move loading
Cell counting board is set on platform, and industrial camera is arranged in microscopical observation end, and industrial camera and high-performance computer are electrically connected.
The principle of the invention:
One, it includes " kidney " module in device that a kind of 3D stem cell emulation culture device of the present invention, which copies human internal organs,
(the first peristaltic pump and second is wriggled for (dialyzer), " lung " module (gas-exchange membrane), " spleen " module (volumetric flask), " heart " module
Pump), culture module (Tissue Culture Flask) and continuous centrifugal machine cultivate the cells, " kidney " module can produce cell metabolism
Raw waste material is discharged to outside device, and the exhaust gas that cell metabolism generates can be discharged to outside device by " lung " module, and " spleen " module exists
Play the role of adjusting in device." heart " module provides endlessly power during cell culture, makes whole device
In liquid circulation is realized as the blood of human body.The modules such as " lung ", " kidney ", " spleen ", " heart " provide one for cell culture
A good external environment so that cell must cultivate it is more stable.Cultivating module then is mainly Tissue Culture Flask, is cell culture
When store cell Primary containers.Each parameter index in culture apparatus can be monitored in incubation in real time, then arranged by control
It applies and achievees the purpose that real-time control.Using continuous centrifugal machine, cell separation can be achieved with while liquid injection.Separate it
Drop is injected in cell counting board using liquid-transfering gun control instrument hand afterwards, the visual field that microscope will be observed that by industrial camera
To high-performance computer, high-performance computer counts to achieve the purpose that count automatically by vision algorithm.
It two, is air pump, constant flow pump, peristaltic pump and pump in pump apparatus of the present invention, air pump and constant flow pump are of the invention a kind of
3D stem cell emulates the input setting of culture device, which passes through air pump input air, calcium liquid, cleaning solution, culture solution, colloidal sol
Liquid and freezing liquid, and constant flow pump input is then cell and glue.In the inoculating cell stage, Tissue Culture Flask and the first lid are connected,
Using the headkerchief with funnel, its purpose is that preventing larger particles from entering in device.Peristaltic pump can be such that the liquid in device is in
The state of flowing, the bounce just as " heart " is general, pump with " kidney " module or " lung " block combiner together with completion " kidney " and
The function of " lung ".By refrigerator, heating sheet, the first valve, the second valve, third valve, the 4th valve, the 5th valve, the 6th valve, the first pressure regulation
Valve, the second pressure regulator valve, the first triple valve and the second triple valve composition control equipment, various valves then control in device liquid and
The flowing of gas, and the substance for using check valve to make these inflow devices will not flow back away, pressure regulator valve (the first pressure regulator valve and
Second pressure regulator valve) it then can control pressure in device, it is ensured that and the cell of culture is in suitable pressure limit, common valve
(the first valve to the 6th valve) and triple valve (the first triple valve and the second triple valve) control the on-off of pipeline then to control liquid
Or the flow direction of gas.Measuring device then includes temperature sensor, pressure sensor and liquidometer, they are respectively used to measuring device
In temperature, pressure, liquid level to being monitored in real time to device, control equipment is controlled according to the data of monitoring, is realized
The effect of real-time control.
Three, the present invention uses continuous centrifugal machine.Other solution cannot be added in general centrifuge during centrifugation.
And the maximum feature of continuous centrifugal machine is exactly that other solution can be added during centrifugation to be centrifuged together.Same stepping can be achieved
The processes such as liquid, drain, stirring and centrifugation.Continuous centrifugal machine is connected in the system of cell culture, is completed in cell culture
Later, cell is separated using continuous centrifugal machine, is needed not wait for after all cell solutions fully enter centrifuge
Centrifuge is just started to work, and centrifuge can input cell solution on one side, is centrifuged on one side, to make isolated process and training
Feeding process integration.
Four, present invention setting liquid shifting equipment, liquid-transfering gun control instrument hand can manipulate the solution of liquid-transfering gun transfer setting capacity,
And six degree of freedom robotic arm then can control the spatial position of liquid-transfering gun control instrument hand and liquid-transfering gun.The most important function of liquid shifting equipment
Can count the cell of centrifuge separator well from being transferred in cell counting board in separating pipe, full-automatic liquid relief is set
It is standby that pipetting processes is made to eliminate manual operation, so that operation is more simple and direct, accurate.
Five, industrial camera is arranged at microscopical observation end in present invention setting counting equipment, and industrial camera directly connects in height
On Project Computer, moveable stage controls movement by stepper motor, and is able to achieve the function of auto-focusing.When can
Moving stage is controlled be shifted out after, liquid-transfering gun control instrument hand control liquid-transfering gun by drop inject cell counting board on,
Moveable stage by the mobile focusing of control, can observe directly the microscopical visual field by high-performance computer and transport again
With the algorithm of computer vision to the cell count in the visual field, to complete the function of counting automatically, manual operation and people are avoided
Work counts bring error.
Beneficial effects of the present invention:
1, the present invention integrates cell culture, cell separation, cell count process etc., and has full-automatic spy
Property, the efficiency of cell culture can be greatly improved, higher reliability is brought, makes cell culture more safely, conveniently.
2, the internal environment that the present invention imitates human body builds a similar cell culture environment, makes the culture of cell more
Stablize, reliably.
3, present invention real-time monitoring, control in cell cultivation process, can prevent various emergency cases, make cell culture
Environment reaches most suitable situation as far as possible.
4, the present invention is operated using advanced continuous centrifugal machine, it can be achieved that synchronizing feed liquor, drain, stirring and centrifugation etc.,
So that the process integration of cell culture, cell separation.
5, the invention enables full-automatic liquid-transfering gun manipulator robots, so that the process automation of liquid relief, reduces manual operation
Bring error, and keep pipetting processes efficient.
6, the present invention carries out cell count using computer, avoids the error in reading and evaluated error of manual operation, no
Made work amount is only reduced, and to count more accurate.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cell culture of the present invention and isolated device；
Fig. 2 is the first lid structural schematic diagram of cell culture of the present invention and isolated device；
Fig. 3 is the continuous centrifugal machine structural schematic diagram of cell culture of the present invention and isolated device；
Fig. 4 is the structural schematic diagram of liquid shifting equipment of the present invention；
Fig. 5 is the liquid-transfering gun control instrument hand structural schematic diagram of liquid shifting equipment of the present invention；
Fig. 6 is the structural schematic diagram of counting equipment of the present invention.
Specific embodiment
Specific embodiment 1: present embodiment is a kind of 3D stem cell emulation culture device, it include cell culture and
Isolated device, liquid shifting equipment and counting equipment；
The cell culture and isolated device include the first lid 1, the second lid 2, the first pressure regulator valve 3, Tissue Culture Flask 4,
Volumetric flask 5, the first peristaltic pump 6, dialysis liquid material bottle 7, first pump 8, waste material bottle 9, dialyzer 10, the second pressure regulator valve the 11, the 6th are single
Base-material bottle 16, the second peristaltic pump 17, second pump are supported to valve 12, the 5th air pump 13, the 7th check valve 14, the 6th air pump 15, waste liquid
18, gas-exchange membrane 19, third valve 20, continuous centrifugal machine 21, the 4th valve 22, the 6th valve 23, colloidal sol waste liquid material bottle 24, cleaning
Waste liquid calcium wasting liquid material bottle 25, the 5th valve 26, the 5th check valve 27, the 8th air pump 28, the 7th air pump 29, the first triple valve and second
Triple valve；
The inoculating cell stage: Tissue Culture Flask 4 is connect with the first lid 1, and the first lid 1 includes the first air pump 1-1, first unidirectional
Valve 1-2, constant flow pump 1-3, the headkerchief 1-4 with funnel, the first valve 1-5, second one-way valve 1-6 and the second air pump 1-7, with funnel
Headkerchief 1-4 is covered on Tissue Culture Flask 4, the headkerchief 1-4 with funnel and the first air pump 1-1, constant flow pump 1-3 and the second air pump 1-7
Be arranged between connection, the first air pump 1-1 and headkerchief 1-4 with funnel the first check valve 1-2, the second air pump 1-7 with funnel
Second one-way valve 1-6 is set between headkerchief 1-4, the lid connection of Tissue Culture Flask 4 is cleaned by waste liquid calcium wasting liquid material by tee tube
Bottle 25 and waste liquid support base-material bottle 16, and the first valve 1-5 is arranged on the inlet pipeline of tee tube；
Cultivate cell stage: Tissue Culture Flask 4 is connect with the second lid 2, and the second lid 2 is unidirectional including third air pump 2-1, third
Valve 2-2, the headkerchief 2-3 without funnel, the second valve 2-4, the 4th check valve 2-5 and the 4th air pump 2-6；Without the headkerchief 2- of funnel
3 cover on Tissue Culture Flask 4, without the headkerchief 2-3 and third air pump 2-1, the 4th air pump 2-6, continuous centrifugal machine 21 of funnel
With the connection of volumetric flask 5, third air pump 2-1 and without setting third check valve 2-2, the 4th air pump 2-6 between the headkerchief 2-3 of funnel
4th check valve 2-5 is set between the headkerchief 2-3 without funnel, volumetric flask 5 and without the is arranged between the headkerchief 2-3 of funnel
One pressure regulator valve 3, continuous centrifugal machine 21 and without being arranged the second valve 2-4 between the headkerchief 2-3 of funnel, continuous centrifugal machine 21 with
Without setting gradually the 5th valve 26 and the 8th air pump 28, the bottom of Tissue Culture Flask 4 in the connecting pipe between the headkerchief 2-3 of funnel
Portion connects the 7th air pump 29, gas-exchange membrane 19, cleaning waste liquid calcium wasting liquid material bottle 25 and waste liquid and supports base-material bottle 16, the 7th air pump 29
5th check valve 27 is set between Tissue Culture Flask 4, third valve 20 is set between gas-exchange membrane 19 and Tissue Culture Flask 4,
Tissue Culture Flask 4 is supported base-material bottle 16 with cleaning waste liquid calcium wasting liquid material bottle 25 and waste liquid by the second triple valve to connect, continous way
Centrifuge 21 and without the connecting pipe between the headkerchief 2-3 of funnel and the connection between the second triple valve and Tissue Culture Flask 4
Pipeline is connected to by the 4th valve 22, and the waste liquid port of continuous centrifugal machine 21 is connected to colloidal sol waste liquid material bottle 24, colloidal sol waste liquid material bottle
6th valve 23 is set between 24 and continuous centrifugal machine 21, and the top of volumetric flask 5 connects the first peristaltic pump 6, volumetric flask 5 and gas
Exchange membrane 19 connects, and the second peristaltic pump 17 is arranged between volumetric flask 5 and gas-exchange membrane 19, and volumetric flask 5 is connect with dialyzer 10,
Second pressure regulator valve 11 is set between volumetric flask 5 and dialyzer 10, and volumetric flask 5 is connect with the 5th air pump 13 and the 6th air pump 15, is held
6th check valve 12 is set between measuring bottle 5 and the 6th air pump 13, the 7th check valve is set between volumetric flask 5 and the 6th air pump 15
14, dialyzer 10 is supported with the first peristaltic pump 6 and waste liquid by base-material bottle 16 by the first triple valve and is connect, it will be saturating by the first pump 8
Parser 10 is connect with dialysis liquid material bottle 7, and the waste outlet of dialyzer 10 is connected to waste material bottle 9；Gas-exchange membrane 19 and the second pump
18 connections；
The liquid shifting equipment includes six degree of freedom robotic arm 42, liquid-transfering gun control instrument hand 43, liquid-transfering gun 44, stem cell point
Vanning 45 and centrifuge tube 52；Liquid-transfering gun control instrument hand 43 is fixed on six degree of freedom robotic arm 42, and liquid-transfering gun 44 is fixed on shifting
On liquid rifle control instrument hand 43, centrifuge tube 52 is loaded in stem cell charging box 45；
The counting equipment includes moveable stage 46, cell counting board 47, microscope 48, industrial camera 49, Gao Xing
It can computer 50 and stepper motor 51；Moveable stage 46, moveable stage 46 and stepper motor are set on microscope 48
51 are electrically connected, and cell counting board 47 is arranged in moveable stage 46, and industrial camera 49, work is arranged in the observation end of microscope 48
Industry camera 49 and high-performance computer 50 are electrically connected.
Specific embodiment 2: the difference of present embodiment and specific embodiment one is: the continuous centrifugal machine
21 include closed rotary feed liquor module 21-1, swing rabbling mechanism 21-2, rotatable platform 21-3, transmission mechanism 21-4, hollow master
Axis 21-5, driving motor 21-6 and conducting slip ring 21-7；Driving motor 21-6 is arranged close to conducting slip ring 21-7, hollow spindle
The bottom end 21-5 is fixedly mounted on conducting slip ring 21-7 rotor shaft, the top of hollow spindle 21-5 pass through rotatable platform 21-6 with
The bottom end of closed rotary feed liquor module 21-1 is fixed, and the lateral wall of hollow spindle 21-5 is fixedly connected with rotatable platform 21-3, rotation
Turn closing feed liquor module 21-1 shell to be fixedly connected with fixed frame, the output end of driving motor 21-6 shaft passes through transmission mechanism
21-4 is connect with hollow spindle 21-5, and driving motor 21-6 drives hollow spindle 21-5 rotation by transmission mechanism 21-4, is swung
Rabbling mechanism 21-2 is mounted on rotatable platform 21-3, and each outlet end of closed rotary feed liquor module 21-1 passes through pipeline respectively
It is tightly connected with an arrival end for swinging rabbling mechanism 21-2, each bottom inlet end point of closed rotary feed liquor module 21-1
Not Tong Guo pipeline and swing rabbling mechanism 21-2 outlet end be tightly connected, the outlet at bottom end of closed rotary feed liquor module 21-1
It is connect with the end sealing of hollow spindle 21-5.Other are same as the specific embodiment one.
Specific embodiment 3: one of present embodiment and specific embodiment one or two difference are: the liquid-transfering gun
Control instrument hand 43 includes that mounting rack 43-13, controller, pressing mechanism, clip claw mechanism and fixed plate 43-14, pressing mechanism are set
It sets in the upper end of mounting rack 43-13, pressing mechanism includes motor fixed bin 43-1, pressure sensor isolation 43-2, compression bar 43-
3, lead screw motor 43-4, linear guide 43-10 and pressure sensor 43-12, lead screw motor 43-4 are arranged vertically downwards,
Lead screw motor 43-4 is affixed by motor fixed bin and the upper end of mounting rack 43-1, and compression bar 43-3 is horizontally disposed, lead screw motor 43-
The middle part of 4 output end and compression bar 43-3 is affixed, and the lower end surface of the front end compression bar 43-3 is equipped with pressure sensor isolation 43-2,
Pressure sensor 43-12 is arranged on mounting rack 43-13, and linear guide 43-10 is fixed in mounting rack 43-13 along the vertical direction
On, rear end and the linear guide 43-10 of compression bar 43-3 is slidably connected, and the pressure signal transmission of pressure sensor isolation 43-2 is given
Pressure sensor 43-12, pressure sensor 43-12 and lead screw motor 43-4 are connect with controller respectively, and clip claw mechanism level is set
It sets in the lower end of mounting rack 43-13；Liquid-transfering gun control instrument hand 43 is fixed on six degree of freedom robotic arm by fixed plate 43-14
On 42.Other are the same as one or two specific embodiments.
Specific embodiment 4: the difference of present embodiment and specific embodiment three is: the clip claw mechanism includes
Direct current generator 43-11, gear mechanism 43-8, two clamping plate 43-6, two clamping jaw fixed plate 43-9 and link mechanism 43-7, two
Clamping jaw fixed plate 43-9 level is oppositely arranged on the lower end of mounting rack 43-13, the output end and gear mechanism of direct current generator 43-11
43-8 connection, gear mechanism 43-8 connection are horizontally set between two clamping jaw fixed plates 9, gear mechanism 43-8 and link mechanism
The front end of 43-7 connection, link mechanism 43-7 is equipped with clamping plate 6, and two clamping plates 6 are oppositely arranged, and direct current generator 11 and controller connect
It connects；Liquid-transfering gun 44 is fixed on liquid-transfering gun control instrument hand 43 by two clamping plates 6.Other and specific embodiment three-phase
Together.
Specific embodiment 5: one of present embodiment and specific embodiment one to four difference are: the described 1st
I 21-1 of pipeline of port valve supports base-material bottle 16 with waste liquid and is connected to, and II 21-2 of pipeline is connected to dialyzer 10, pipeline III 21-3 and first
Peristaltic pump 6 is connected to.Other are identical as specific embodiment one to four.
Specific embodiment 6: one of present embodiment and specific embodiment one to five difference are: the described 2nd 3
IV 31-1 of pipeline of port valve is connected to Tissue Culture Flask 4, and V 31-2 of pipeline and waste liquid are supported base-material bottle 16 and be connected to, VI 31-3 of pipeline and
Wash the connection of waste liquid calcium wasting liquid material bottle 25.Other are identical as specific embodiment one to five.
Specific embodiment 7: one of present embodiment and specific embodiment one to six difference are: a kind of 3D
Stem cell emulation culture device further includes refrigerator and heating sheet, by refrigerator, heating sheet, the first valve 1-5, the second valve 2-4, the
Three valves 20, the 4th valve 22, the 5th valve 26, the 6th valve 23, the first pressure regulator valve 3, the second pressure regulator valve 11, the first triple valve and the two or three
Refrigerator and heating sheet is respectively set in Tissue Culture Flask 4 and volumetric flask 5 in port valve composition control equipment.Other and specific reality
It is identical to apply mode one to six.
Specific embodiment 8: one of present embodiment and specific embodiment one to seven difference are: a kind of 3D
It further includes measuring device that stem cell, which emulates culture device, and the measuring device includes temperature sensor, pressure sensor and liquid level
Meter, is arranged temperature sensor in Tissue Culture Flask 4 and volumetric flask 5, and pressure sensor is arranged in the input terminal of constant flow pump 1-3,
Pressure sensor is set in the input terminal of the first pressure regulator valve 3 and the second pressure regulator valve 11, liquidometer is set in volumetric flask 5.Other
It is identical as specific embodiment one to seven.
Specific embodiment 9: one of present embodiment and specific embodiment one to eight difference are: the dialyzer
10 be hollow fiber dialyzer.Other are identical as specific embodiment one to eight.
The content of present invention is not limited only to the content of the respective embodiments described above, the group of one of them or several specific embodiments
The purpose of invention also may be implemented in contract sample.
Using following verification experimental verifications effect of the present invention
Embodiment 1: a kind of 3D stem cell emulation culture device, it includes cell culture and isolated device, liquid shifting equipment
And counting equipment；
The cell culture and isolated device include the first lid 1, the second lid 2, the first pressure regulator valve 3, Tissue Culture Flask 4,
Volumetric flask 5, the first peristaltic pump 6, dialysis liquid material bottle 7, first pump 8, waste material bottle 9, dialyzer 10, the second pressure regulator valve the 11, the 6th are single
Base-material bottle 16, the second peristaltic pump 17, second pump are supported to valve 12, the 5th air pump 13, the 7th check valve 14, the 6th air pump 15, waste liquid
18, gas-exchange membrane 19, third valve 20, continuous centrifugal machine 21, the 4th valve 22, the 6th valve 23, colloidal sol waste liquid material bottle 24, cleaning
Waste liquid calcium wasting liquid material bottle 25, the 5th valve 26, the 5th check valve 27, the 8th air pump 28, the 7th air pump 29, the first triple valve and second
Triple valve；
The inoculating cell stage: Tissue Culture Flask 4 is connect with the first lid 1, and the first lid 1 includes the first air pump 1-1, first unidirectional
Valve 1-2, constant flow pump 1-3, the headkerchief 1-4 with funnel, the first valve 1-5, second one-way valve 1-6 and the second air pump 1-7, with funnel
Headkerchief 1-4 is covered on Tissue Culture Flask 4, the headkerchief 1-4 with funnel and the first air pump 1-1, constant flow pump 1-3 and the second air pump 1-7
Be arranged between connection, the first air pump 1-1 and headkerchief 1-4 with funnel the first check valve 1-2, the second air pump 1-7 with funnel
Second one-way valve 1-6 is set between headkerchief 1-4, the lid connection of Tissue Culture Flask 4 is cleaned by waste liquid calcium wasting liquid material by tee tube
Bottle 25 and waste liquid support base-material bottle 16, and the first valve 1-5 is arranged on the inlet pipeline of tee tube；
Cultivate cell stage: Tissue Culture Flask 4 is connect with the second lid 2, and the second lid 2 is unidirectional including third air pump 2-1, third
Valve 2-2, the headkerchief 2-3 without funnel, the second valve 2-4, the 4th check valve 2-5 and the 4th air pump 2-6；Without the headkerchief 2- of funnel
3 cover on Tissue Culture Flask 4, without the headkerchief 2-3 and third air pump 2-1, the 4th air pump 2-6, continuous centrifugal machine 21 of funnel
With the connection of volumetric flask 5, third air pump 2-1 and without setting third check valve 2-2, the 4th air pump 2-6 between the headkerchief 2-3 of funnel
4th check valve 2-5 is set between the headkerchief 2-3 without funnel, volumetric flask 5 and without the is arranged between the headkerchief 2-3 of funnel
One pressure regulator valve 3, continuous centrifugal machine 21 and without being arranged the second valve 2-4 between the headkerchief 2-3 of funnel, continuous centrifugal machine 21 with
Without setting gradually the 5th valve 26 and the 8th air pump 28, the bottom of Tissue Culture Flask 4 in the connecting pipe between the headkerchief 2-3 of funnel
Portion connects the 7th air pump 29, gas-exchange membrane 19, cleaning waste liquid calcium wasting liquid material bottle 25 and waste liquid and supports base-material bottle 16, the 7th air pump 29
5th check valve 27 is set between Tissue Culture Flask 4, third valve 20 is set between gas-exchange membrane 19 and Tissue Culture Flask 4,
Tissue Culture Flask 4 is supported base-material bottle 16 with cleaning waste liquid calcium wasting liquid material bottle 25 and waste liquid by the second triple valve to connect, continous way
Centrifuge 21 and without the connecting pipe between the headkerchief 2-3 of funnel and the connection between the second triple valve and Tissue Culture Flask 4
Pipeline is connected to by the 4th valve 22, and the waste liquid port of continuous centrifugal machine 21 is connected to colloidal sol waste liquid material bottle 24, colloidal sol waste liquid material bottle
6th valve 23 is set between 24 and continuous centrifugal machine 21, and the top of volumetric flask 5 connects the first peristaltic pump 6, volumetric flask 5 and gas
Exchange membrane 19 connects, and the second peristaltic pump 17 is arranged between volumetric flask 5 and gas-exchange membrane 19, and volumetric flask 5 is connect with dialyzer 10,
Second pressure regulator valve 11 is set between volumetric flask 5 and dialyzer 10, and volumetric flask 5 is connect with the 5th air pump 13 and the 6th air pump 15, is held
6th check valve 12 is set between measuring bottle 5 and the 6th air pump 13, the 7th check valve is set between volumetric flask 5 and the 6th air pump 15
14, dialyzer 10 is supported with the first peristaltic pump 6 and waste liquid by base-material bottle 16 by the first triple valve and is connect, it will be saturating by the first pump 8
Parser 10 is connect with dialysis liquid material bottle 7, and the waste outlet of dialyzer 10 is connected to waste material bottle 9；Gas-exchange membrane 19 and the second pump
18 connections；
The liquid shifting equipment includes six degree of freedom robotic arm 42, liquid-transfering gun control instrument hand 43, liquid-transfering gun 44, stem cell point
Vanning 45 and centrifuge tube 52；Liquid-transfering gun control instrument hand 43 is fixed on six degree of freedom robotic arm 42, and liquid-transfering gun 44 is fixed on shifting
On liquid rifle control instrument hand 43, centrifuge tube 52 is loaded in stem cell charging box 45；
The counting equipment includes moveable stage 46, cell counting board 47, microscope 48, industrial camera 49, Gao Xing
It can computer 50 and stepper motor 51；Moveable stage 46, moveable stage 46 and stepper motor are set on microscope 48
51 are electrically connected, and cell counting board 47 is arranged in moveable stage 46, and industrial camera 49, work is arranged in the observation end of microscope 48
Industry camera 49 and high-performance computer 50 are electrically connected.
The continuous centrifugal machine 21 includes closed rotary feed liquor module 21-1, swings rabbling mechanism 21-2, rotatable platform
21-3, transmission mechanism 21-4, hollow spindle 21-5, driving motor 21-6 and conducting slip ring 21-7；Driving motor 21-6 is close to be led
Electric slip ring 21-7 setting, the bottom end hollow spindle 21-5 are fixedly mounted on conducting slip ring 21-7 rotor shaft, hollow spindle 21-5
Top pass through rotatable platform 21-6 and the bottom end of closed rotary feed liquor module 21-1 and fix, the lateral wall of hollow spindle 21-5 and
Rotatable platform 21-3 is fixedly connected, and closed rotary feed liquor module 21-1 shell is fixedly connected with fixed frame, and driving motor 21-6 turns
The output end of axis is connect by transmission mechanism 21-4 with hollow spindle 21-5, and driving motor 21-6 is driven by transmission mechanism 21-4
Hollow spindle 21-5 rotation swings rabbling mechanism 21-2 and is mounted on rotatable platform 21-3, closed rotary feed liquor module 21-1's
Each outlet end passes through pipeline respectively and swings the arrival end sealed connection of rabbling mechanism 21-2, closed rotary feed liquor module
Each bottom inlet end of 21-1 pass through respectively pipeline and swing rabbling mechanism 21-2 outlet end be tightly connected, closed rotary into
The outlet at bottom end of liquid module 21-1 is connect with the end sealing of hollow spindle 21-5.
The liquid-transfering gun control instrument hand 43 includes mounting rack 43-13, controller, pressing mechanism, clip claw mechanism and fixation
The upper end of mounting rack 43-13 is arranged in plate 43-14, pressing mechanism, and pressing mechanism includes motor fixed bin 43-1, pressure sensor
Isolation 43-2, compression bar 43-3, lead screw motor 43-4, linear guide 43-10 and pressure sensor 43-12, lead screw motor 43-4
It is arranged vertically downwards, lead screw motor 43-4 is affixed by motor fixed bin and the upper end of mounting rack 43-1, compression bar 43-3
Horizontally disposed, the middle part of the output end and compression bar 43-3 of lead screw motor 43-4 is affixed, and the lower end surface of the front end compression bar 43-3 is equipped with
Pressure sensor isolation 43-2, pressure sensor 43-12 setting is on mounting rack 43-13, and linear guide 43-10 is along vertical side
To being fixed on mounting rack 43-13, rear end and the linear guide 43-10 of compression bar 43-3 is slidably connected, pressure sensor isolation
The pressure signal transmission of 43-2 give pressure sensor 43-12, pressure sensor 43-12 and lead screw motor 43-4 respectively with controller
Connection, clip claw mechanism are horizontally set on the lower end of mounting rack 43-13；By fixed plate 43-14 by liquid-transfering gun control instrument hand 43
It is fixed on six degree of freedom robotic arm 42.
The clip claw mechanism includes that direct current generator 43-11, gear mechanism 43-8, two clamping plate 43-6, two clamping jaws are fixed
Plate 43-9 and link mechanism 43-7, two clamping jaw fixed plate 43-9 levels are oppositely arranged on the lower end of mounting rack 43-13, direct current
The output end of machine 43-11 is connect with gear mechanism 43-8, gear mechanism 43-8 connection be horizontally set on two clamping jaw fixed plates 9 it
Between, gear mechanism 43-8 is connect with link mechanism 43-7, and the front end of link mechanism 43-7 is equipped with clamping plate 6, and two clamping plates 6 are opposite to be set
It sets, direct current generator 11 is connect with controller；Liquid-transfering gun 44 is fixed on liquid-transfering gun control instrument hand 43 by two clamping plates 6.
I 21-1 of pipeline of first triple valve supports base-material bottle 16 with waste liquid and is connected to, and II 21-2 of pipeline and dialyzer 10 connect
Logical, III 21-3 of pipeline is connected to the first peristaltic pump 6.
IV 31-1 of pipeline of second triple valve is connected to Tissue Culture Flask 4, and V 31-2 of pipeline and waste liquid support base-material bottle
16 connections, VI 31-3 of pipeline are connected to waste liquid calcium wasting liquid material bottle 25 is washed.
A kind of application method of 3D stem cell emulation culture device, detailed process is as follows:
One, the inoculating cell stage: Tissue Culture Flask 4 is connect with the first lid 1；
1, inject calcium liquid: the first valve 1-5 being opened, then opens the second air pump 1-7, the second triple valve by V 31-2 of pipeline and
VI 31-3 of pipeline connection injects calcium liquid by the second air pump 1-7, the time required to reach after stop, closing timer, close the
Two air pump 1-7；The purpose of calcium liquid is to make glue be set in cell surface；
2, inoculating cell: constant flow pump 1-3 is opened, and is injected cell and glue simultaneously respectively by constant flow pump 1-3, is worked as injection rate
Stop after reaching necessary requirement and close constant flow pump 1-3, measure the pressure of constant flow pump 1-3 respectively in the process and is controlled
System；
3, it excludes calcium liquid: the first air pump 1-1 being opened, then closes the first valve 1-5, the second triple valve is by IV 31-1 of pipeline and pipe
VI 31-3 of road connection excludes calcium liquid by inflating into the first air pump 1-1, stops after reaching required time, closes the first gas
Pump 1-1；
Two, it cultivates cell stage: changing lid and Tissue Culture Flask 4 is allowed to connect with the second lid 2,
4, cleaning calcium liquid residual: the 4th air pump 2-6 is opened, the second triple valve connects by IV 31-1 of pipeline and VI 31-3 of pipeline
It is logical, cleaning solution is injected by the 4th air pump 2-6, is stopped after reaching required time, the 4th air pump 2-6 is closed；
5, it excludes cleaning solution residual: third air pump 2-1 being opened, the second triple valve is by VI 31-3 of IV 31-1 of pipeline and pipeline
Connection excludes cleaning solution residual by inflating into third air pump 2-1, stops after reaching required time, close third air pump 2-
1；Second triple valve is connected to by V 31-2 of pipeline with VI 31-3 of pipeline；
6, it injects culture solution: opening the 5th air pump 13 and inject cleaning solution, open the 6th air pump 15 and inject culture solution, use liquid level
Meter monitoring 5 liquid level of volumetric flask, reaches the stopping of liquidometer upper limit；
7, culture circulation: the first pump 8 and the second pump 18 are opened, and realize that the function of " kidney " exchange waste liquid and " lung " exchange respectively
The function of exhaust gas opens the first peristaltic pump 6 and the second peristaltic pump 17, enables liquid flow circuit in device, such as the heart of people
Dirty bounce drives the flowing of blood, opens third valve 20, cell is cultivated 15 days in Tissue Culture Flask 4, during culture
Dynamic control is carried out to temperature, pressure, liquid level；
8, stop circulation: closing the first pump 8 and the second pump 18, close the first peristaltic pump 6 and the second peristaltic pump 17, close the
Three valves 20 close the 5th air pump 13 and the 6th air pump 15, terminate entire cell cultivation process；
9, it excluding culture solution: opening third air pump 2-1, the second triple valve is connected to by IV 31-1 of pipeline with V 31-2 of pipeline,
It is inflated by third air pump 2-1 and excludes culture solution, stopped after reaching required time, close third air pump 2-1, the second triple valve
V 31-2 of pipeline is allowed to be connected to VI 31-3 of pipeline；
10, inject sol solutions: the effect of sol solutions is the glue for dissolving cell surface, and continuous centrifugal machine 21 is first allowed to work,
The 7th air pump 29 is opened again, opens the second valve 2-4, and sol solutions are injected by the 7th air pump 29, are stopped after reaching required time,
And close the 7th air pump 29 and the second valve 2-4；It needs to carry out dynamic control to continuous centrifugal machine 21 during this；
11, sol solutions are excluded: third air pump 2-1 is opened, is inflated by third air pump 2-1 and excludes sol solutions, open the
Two valve 2-4, the second valve of phase down 2-4 after the time required to reaching；The 4th valve 22 is opened, stops closing after the time required to reaching
The 4th valve 22 of make and break closes third air pump 2-1 after finally reaching the time for excluding sol solutions；
12, injection cleaning solution is cleaned: being opened the 4th air pump 2-6, is opened the 4th valve 22 and the 6th valve 23, pass through the 4th
Air pump 2-6 injects cleaning solution, reaches the 4th air pump 2-6 of phase down after required time, and close the 4th valve 22 and the 6th valve 23；
13, inject freezing liquid: the 8th air pump is first opened in the protection carried out when freezing liquid is to refrigerate cell to cell
28, the 5th valve 26 is opened, freezing liquid is injected by the 8th air pump 28, reaches after the time the 8th air pump 28 of phase down and closes the
Five valves 26；
14, the centrifuge tube 52 on continuous centrifugal machine 21 is taken apart, is exactly the culture separated in centrifuge tube 52
Centrifuge tube 52 is put into stem cell charging box 45 by cell；
15, moveable stage 46 is controlled using stepper motor 51 to be moved, operated with liquid-transfering gun control instrument hand 43
Liquid-transfering gun 44 is sampled out of centrifuge tube 52 to be instilled in cell counting board 47, is controlled 46 auto-focusing of moveable stage again, is used
Industrial camera 48 is observed, and is counted using high-performance computer 50；Again with liquid-transfering gun control instrument hand 43 to being carried out in centrifuge tube 52
Encapsulation.
Claims (9)
1. a kind of 3D stem cell emulates culture device, it is characterised in that a kind of 3D stem cell emulation culture device includes cell culture
With isolated device, liquid shifting equipment and counting equipment；
The cell culture and isolated device include the first lid (1), the second lid (2), the first pressure regulator valve (3), Tissue Culture Flask
(4), volumetric flask (5), the first peristaltic pump (6), dialysis liquid material bottle (7), the first pump (8), waste material bottle (9), dialyzer (10), second
Pressure regulator valve (11), the 6th check valve (12), the 5th air pump (13), the 7th check valve (14), the 6th air pump (15), waste liquid support base-material
Bottle (16), the second peristaltic pump (17), second pump (18), gas-exchange membrane (19), third valve (20), continuous centrifugal machine (21),
4th valve (22), the 6th valve (23), colloidal sol waste liquid material bottle (24), cleaning waste liquid calcium wasting liquid material bottle (25), the 5th valve (26), the 5th
Check valve (27), the 8th air pump (28), the 7th air pump (29), the first triple valve, the second triple valve, control equipment and measurement are set
It is standby；
The inoculating cell stage: Tissue Culture Flask (4) is connect with the first lid (1), and the first lid (1) includes the first air pump (1-1), first
Check valve (1-2), constant flow pump (1-3), the headkerchief (1-4) with funnel, the first valve (1-5), second one-way valve (1-6) and the second gas
It pumps (1-7), the headkerchief (1-4) with funnel covers on Tissue Culture Flask (4), the headkerchief (1-4) with funnel and the first air pump (1-
1), constant flow pump (1-3) and the second air pump (1-7) connection are arranged the between the first air pump (1-1) and headkerchief (1-4) with funnel
Second one-way valve (1-6) is set between one check valve (1-2), the second air pump (1-7) and headkerchief (1-4) with funnel, passes through three
Base-material bottle (16) are supported in lid connection cleaning waste liquid calcium wasting liquid material bottle (25) and waste liquid of the siphunculus by Tissue Culture Flask (4), in tee tube
Inlet pipeline on the first valve (1-5) is set；
Culture cell stage: Tissue Culture Flask (4) is connect with the second lid (2), and the second lid (2) includes third air pump (2-1), third
Check valve (2-2), the headkerchief (2-3) without funnel, the second valve (2-4), the 4th check valve (2-5) and the 4th air pump (2-6)；No
Headkerchief (2-3) with funnel covers on Tissue Culture Flask (4), without the headkerchief (2-3) and third air pump (2-1), the 4th of funnel
Air pump (2-6), continuous centrifugal machine (21) and volumetric flask (5) connection, third air pump (2-1) and the headkerchief (2-3) without funnel
Between be arranged third check valve (2-2), the 4th air pump (2-6) with without between the headkerchief (2-3) of funnel setting the 4th check valve
(2-5), volumetric flask (5) and is arranged the first pressure regulator valve (3) without between the headkerchief (2-3) of funnel, continuous centrifugal machine (21) with
It is arranged the second valve (2-4) without between the headkerchief (2-3) of funnel, continuous centrifugal machine (21) and the headkerchief (2-3) without funnel
Between connecting pipe on set gradually the 5th valve (26) and the 8th air pump (28), the bottom connection the 7th of Tissue Culture Flask (4)
Air pump (29), gas-exchange membrane (19), cleaning waste liquid calcium wasting liquid material bottle (25) and waste liquid support base-material bottle (16), the 7th air pump (29)
5th check valve (27) is set between Tissue Culture Flask (4), the is arranged between gas-exchange membrane (19) and Tissue Culture Flask (4)
Base-material bottle is supported by the second triple valve in Tissue Culture Flask (4) and cleaning waste liquid calcium wasting liquid material bottle (25) and waste liquid by three valves (20)
(16) connect, continuous centrifugal machine (21) and without between the headkerchief (2-3) of funnel connecting pipe and the second triple valve and carefully
Connecting pipe between born of the same parents' culture bottle (4) is connected to by the 4th valve (22), and the waste liquid port and colloidal sol of continuous centrifugal machine (21) are useless
Liquid material bottle (24) connection, is arranged the 6th valve (23), volumetric flask (5) between colloidal sol waste liquid material bottle (24) and continuous centrifugal machine (21)
Top connect the first peristaltic pump (6), volumetric flask (5) connect with gas-exchange membrane (19), volumetric flask (5) and gas-exchange membrane
(19) be arranged between the second peristaltic pump (17), volumetric flask (5) is connect with dialyzer (10), volumetric flask (5) and dialyzer (10) it
Between be arranged the second pressure regulator valve (11), volumetric flask (5) is connect with the 5th air pump (13) and the 6th air pump (15), volumetric flask (5) and the
6th check valve (12) is set between six air pumps (13), the 7th check valve is set between volumetric flask (5) and the 6th air pump (15)
(14), dialyzer (10) is supported base-material bottle (16) with the first peristaltic pump (6) and waste liquid by the first triple valve to connect, passes through first
Dialyzer (10) is connect by pump (8) with dialysis liquid material bottle (7), and the waste outlet of dialyzer (10) is connected to waste material bottle (9)；Gas
Exchange membrane (19) is connect with the second pump (18)；
The liquid shifting equipment includes six degree of freedom robotic arm (42), liquid-transfering gun control instrument hand (43), liquid-transfering gun (44), stem cell
Charging box (45) and centrifuge tube (52)；Liquid-transfering gun control instrument hand (43) is fixed on six degree of freedom robotic arm (42), liquid-transfering gun
(44) it is fixed on liquid-transfering gun control instrument hand (43), centrifuge tube (52) loads in stem cell charging box (45)；
The counting equipment include moveable stage (46), cell counting board (47), microscope (48), industrial camera (49),
High-performance computer (50) and stepper motor (51)；Moveable stage (46) are set on microscope (48), moveable stage
(46) it is electrically connected with stepper motor (51), cell counting board (47) is set in moveable stage (46), microscope (48)
End setting industrial camera (49) is observed, industrial camera (49) and high-performance computer (50) are electrically connected.
2. a kind of 3D stem cell according to claim 1 emulates culture device, it is characterised in that the continuous centrifugal machine
(21) include closed rotary feed liquor module (21-1), swing rabbling mechanism (21-2), rotatable platform (21-3), transmission mechanism (21-
4), hollow spindle (21-5), driving motor (21-6) and conducting slip ring (21-7)；Driving motor (21-6) is close to conducting slip ring
(21-7) setting, the bottom end hollow spindle (21-5) are fixedly mounted on conducting slip ring (21-7) rotor shaft, hollow spindle (21-
5) top passes through rotatable platform (21-6) and the bottom end of closed rotary feed liquor module (21-1) is fixed, hollow spindle (21-5)
Lateral wall is fixedly connected with rotatable platform (21-3), and closed rotary feed liquor module (21-1) shell is fixedly connected with fixed frame, is driven
The output end of dynamic motor (21-6) shaft is connect by transmission mechanism (21-4) with hollow spindle (21-5), driving motor (21-6)
Hollow spindle (21-5) rotation is driven by transmission mechanism (21-4), rabbling mechanism (21-2) is swung and is mounted on rotatable platform (21-
3) on, each outlet end of closed rotary feed liquor module (21-1) passes through one of pipeline and swing rabbling mechanism (21-2) respectively
Arrival end is tightly connected, and each bottom inlet end of closed rotary feed liquor module (21-1) passes through pipeline respectively and swings blender
The outlet end of structure (21-2) is tightly connected, outlet at bottom end and hollow spindle (21-5) of closed rotary feed liquor module (21-1)
End sealing connection.
3. a kind of 3D stem cell according to claim 1 emulates culture device, it is characterised in that the liquid-transfering gun executor
Tool hand (43) includes mounting rack (43-13), controller, pressing mechanism, clip claw mechanism and fixed plate (43-14), and pressing mechanism is set
Set in the upper end of mounting rack (43-13), pressing mechanism include motor fixed bin (43-1), pressure sensor isolation (43-2),
Compression bar (43-3), lead screw motor (43-4), linear guide (43-10) and pressure sensor (43-12), the edge lead screw motor (43-4)
Vertical direction is down-set, and lead screw motor (43-4) is affixed by motor fixed bin and the upper end of mounting rack (43-1), compression bar
(43-3) is horizontally disposed, and the middle part of the output end and compression bar (43-3) of lead screw motor (43-4) is affixed, the front end compression bar (43-3)
Lower end surface is equipped with pressure sensor isolation (43-2), and pressure sensor (43-12) is arranged on mounting rack (43-13), directly
Line guide rail (43-10) is fixed in along the vertical direction on mounting rack (43-13), the rear end of compression bar (43-3) and linear guide (43-
10) it is slidably connected, the pressure signal transmission of pressure sensor isolation (43-2) gives pressure sensor (43-12), pressure sensing
Device (43-12) and lead screw motor (43-4) are connect with controller respectively, and clip claw mechanism is horizontally set under mounting rack (43-13)
End；Liquid-transfering gun control instrument hand (43) is fixed on six degree of freedom robotic arm (42) by fixed plate (43-14).
4. a kind of 3D stem cell emulates culture device according to claim 3, it is characterised in that the clip claw mechanism includes straight
Galvanic electricity machine (43-11), gear mechanism (43-8), two clamping plates (43-6), two clamping jaw fixed plates (43-9) and link mechanism
(43-7), two clamping jaw fixed plate (43-9) levels are oppositely arranged on the lower end of mounting rack (43-13), direct current generator (43-11)
Output end connect with gear mechanism (43-8), gear mechanism (43-8) connection be horizontally set on two clamping jaw fixed plates (9) it
Between, gear mechanism (43-8) is connect with link mechanism (43-7), and the front end of link mechanism (43-7) is equipped with clamping plate (6), two folders
Plate (6) is oppositely arranged, and direct current generator (11) is connect with controller；Liquid-transfering gun (44) is fixed on liquid relief by two clamping plates (6)
On rifle control instrument hand (43).
5. a kind of 3D stem cell emulates culture device according to claim 3, it is characterised in that the pipe of first triple valve
Road I (21-1) and waste liquid are supported base-material bottle (16) and be connected tos, and pipeline II (21-2) is connected to dialyzer (10), pipeline III (21-3) and the
One peristaltic pump (6) connection.
6. a kind of 3D stem cell emulates culture device according to claim 3, it is characterised in that the pipe of second triple valve
Road IV (31-1) is connected to Tissue Culture Flask (4), and pipeline V (31-2) is supported base-material bottle (16) with waste liquid and is connected to, pipeline VI (31-3)
It is connected to waste liquid calcium wasting liquid material bottle (25) is washed.
7. a kind of 3D stem cell emulates culture device according to claim 1, it is characterised in that a kind of 3D stem cell is imitative
True culture device further includes refrigerator and heating sheet, by refrigerator, heating sheet, the first valve (1-5), the second valve (2-4), third valve
(20), the 4th valve (22), the 5th valve (26), the 6th valve (23), the first pressure regulator valve (3), the second pressure regulator valve (11), the first triple valve
With the second triple valve composition control equipment, refrigerator and heating sheet is respectively set in Tissue Culture Flask (4) and volumetric flask (5).
8. a kind of 3D stem cell emulates culture device according to claim 7, it is characterised in that a kind of 3D stem cell is imitative
True culture device further includes measuring device, and the measuring device includes temperature sensor, pressure sensor and liquidometer, in cell
Temperature sensor is set in culture bottle (4) and volumetric flask (5), pressure sensor is set in the input terminal of constant flow pump (1-3), the
Pressure sensor is arranged in the input terminal of one pressure regulator valve (3) and the second pressure regulator valve (11), and liquidometer is arranged in volumetric flask (5).
9. a kind of 3D stem cell emulates culture device according to claim 1, it is characterised in that the dialyzer (10) is sky
Core fiber dialyzer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910408089.8A CN110129200A (en) | 2019-05-15 | 2019-05-15 | A kind of 3D stem cell emulation culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910408089.8A CN110129200A (en) | 2019-05-15 | 2019-05-15 | A kind of 3D stem cell emulation culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110129200A true CN110129200A (en) | 2019-08-16 |
Family
ID=67574644
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910408089.8A Pending CN110129200A (en) | 2019-05-15 | 2019-05-15 | A kind of 3D stem cell emulation culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110129200A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107051A (en) * | 2021-11-26 | 2022-03-01 | 长春祈健生物制品有限公司 | Process for preparing biological product by using continuous flow centrifugal machine |
| WO2024011770A1 (en) * | 2022-07-10 | 2024-01-18 | 苏州欣协生物科技有限公司 | Cell centrifugal culture apparatus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070238169A1 (en) * | 2006-04-11 | 2007-10-11 | The Board Of Trustees Of The Leland Stanford Junior University | Cell sorter and culture system |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| CN102099458A (en) * | 2008-06-05 | 2011-06-15 | 威斯康星医学院公司 | Three dimensional tissues for high-throughput assays |
| CN206940913U (en) * | 2017-07-10 | 2018-01-30 | 山东新华医疗器械股份有限公司 | A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems |
| CN209307394U (en) * | 2018-09-21 | 2019-08-27 | 中国科学院苏州生物医学工程技术研究所 | High-throughput Three-dimensional cell culture system |
-
2019
- 2019-05-15 CN CN201910408089.8A patent/CN110129200A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070238169A1 (en) * | 2006-04-11 | 2007-10-11 | The Board Of Trustees Of The Leland Stanford Junior University | Cell sorter and culture system |
| CN102099458A (en) * | 2008-06-05 | 2011-06-15 | 威斯康星医学院公司 | Three dimensional tissues for high-throughput assays |
| CN102061260A (en) * | 2010-12-01 | 2011-05-18 | 汪华 | Medical automatic cell production device and production method thereof |
| CN206940913U (en) * | 2017-07-10 | 2018-01-30 | 山东新华医疗器械股份有限公司 | A kind of feeding pipeline cleaning and sterilizing system for high-flux cell culture systems |
| CN209307394U (en) * | 2018-09-21 | 2019-08-27 | 中国科学院苏州生物医学工程技术研究所 | High-throughput Three-dimensional cell culture system |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114107051A (en) * | 2021-11-26 | 2022-03-01 | 长春祈健生物制品有限公司 | Process for preparing biological product by using continuous flow centrifugal machine |
| WO2024011770A1 (en) * | 2022-07-10 | 2024-01-18 | 苏州欣协生物科技有限公司 | Cell centrifugal culture apparatus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107674837B (en) | Full-automatic cell culture and analysis load | |
| CN108969839A (en) | A kind of automatic dressing changing device of more bottles of infusions | |
| CN102061260B (en) | Medical automatic cell production device and production method thereof | |
| CN110129200A (en) | A kind of 3D stem cell emulation culture device | |
| CN106367343A (en) | Full-automatic intelligent cell culture device and control method thereof | |
| CN110129192B (en) | Full-automatic cell separation culture system | |
| CN101300339A (en) | Shaker for cell culture and shaken culture system in cell culture method | |
| CN206014938U (en) | Full-automatic cell continuous culture system | |
| CN106893673A (en) | A kind of soil bacteria separates counting experimental provision | |
| CN110231314A (en) | Activated sludge on-line monitoring device | |
| CN106244455B (en) | Fully-automatic intelligent cell culture apparatus and its control method | |
| CN109401962A (en) | A method of preparing cell | |
| CN206635346U (en) | Fully-automatic intelligent cell culture apparatus | |
| CN110186737A (en) | A kind of liquid-based specimens film-making dyeing all-in-one machine | |
| CN206843495U (en) | Ezyme bio-reactor and intelligent enzyme reaction work station | |
| CN106771297B (en) | Automatic sample body-packing based on Sterility testing flow | |
| CN210834642U (en) | Protein detection instrument | |
| CN209405367U (en) | A kind of peritoneal dialysis machine | |
| CN201686700U (en) | Tissue cell culture device | |
| CN215668049U (en) | Biological tissue production system | |
| CN109321457A (en) | A kind of equipment and its application preparing cell | |
| CN216747039U (en) | Full-automatic sample pretreatment device | |
| CN109439542A (en) | A method of preparing cell | |
| CN109321458A (en) | A method of preparing cell | |
| CN212357270U (en) | Automatic IVF culture and time difference analysis device and matched culture dish |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200313 Address after: 1500001 Room 101, building 10, no.1616, Chuangxin Road, Songbei District, Harbin City, Heilongjiang Province Applicant after: Harbin University of Technology International Stem Cell Engineering Research Institute Co.,Ltd. Address before: 150090 No. 130 Changjiang Road, Nangang District, Heilongjiang, Harbin, China Applicant before: Lin Weiyang |
|
| AD01 | Patent right deemed abandoned | ||
| AD01 | Patent right deemed abandoned |
Effective date of abandoning: 20221111 |