CN216747039U - Full-automatic sample pretreatment device - Google Patents
Full-automatic sample pretreatment device Download PDFInfo
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- CN216747039U CN216747039U CN202123453208.3U CN202123453208U CN216747039U CN 216747039 U CN216747039 U CN 216747039U CN 202123453208 U CN202123453208 U CN 202123453208U CN 216747039 U CN216747039 U CN 216747039U
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Abstract
The utility model relates to a full-automatic sample pretreatment device, and belongs to the technical field of machinery. The utility model provides a full-automatic sample pretreatment device, which comprises a sample placing module, a reagent supplying module, a liquid transferring module and a control module, wherein the sample placing module is provided with a test tube fixing device, the test tube fixing device is used for fixing a test tube filled with a sample to be detected, the reagent supplying module comprises a sample pretreatment unit, the sample pretreatment unit is used for placing a reagent bottle filled with a reagent required for sample pretreatment, the liquid transferring module is used for transferring the reagent required for sample pretreatment in the reagent bottle to the test tube filled with the sample to be detected, and the control module is used for controlling the liquid transferring module so as to control the addition amount of the reagent required for sample pretreatment in the test tube filled with the sample to be detected; the device is used for preprocessing the cells to be detected, so that manual intervention can be reduced, and the device has the characteristics of simple operation steps, low labor cost, high processing speed, high operation stability and low operation error rate.
Description
Technical Field
The utility model relates to a full-automatic sample pretreatment device, and belongs to the technical field of machinery.
Background
The flow cytometry technology is a detection technology for carrying out multi-parameter and rapid quantitative analysis on single cells or other biological particles by monoclonal antibodies on the cellular molecular level. The method can analyze tens of thousands of cells at high speed, can simultaneously measure a plurality of parameters from one cell, has the advantages of high speed, high precision and good accuracy, and is one of the most advanced cell quantitative analysis techniques in the present generation.
In clinical and scientific research, especially in clinical flow application, flow detection of white blood cells is very common, and samples are often whole blood or other samples containing a small amount of red blood cells, and if the red blood cells in the system are not removed or lysed, the detection result is greatly interfered, and even the detection cannot be performed at all.
The sample is firstly processed by the lymphocyte separating medium, but the sample is time-consuming and labor-consuming, important cell groups are lost, and then the sample is gradually replaced by the erythrocyte lysate, so that the lysis scheme with high speed, high leukocyte yield and high cell group purity is preferred. Clinically detecting lymphocyte subpopulations, HLA-B27, CD34+ hematopoietic stem cell, car-T monitoring and the like, and after the antibody incubation step is completed, the erythrocyte lysis step is required to be completed to carry out the up-flow detection.
At present, because of lacking the device that can carry out full-automatic red blood cell lysis to the sample and handle, the sample red blood cell lysis of present stage is handled and is mainly accomplished through manual operation, and the step is loaded down with trivial details, the human cost is high, the treatment effeciency is low, the operation is unstable and the operation error rate is high.
Therefore, it is desirable to find a device capable of performing a full-automatic red blood cell lysis treatment on a sample, so as to simplify the operation steps of the red blood cell lysis treatment, reduce the labor cost of the red blood cell lysis treatment, improve the treatment efficiency of the red blood cell lysis treatment, improve the operation stability of the red blood cell lysis treatment, and reduce the operation error rate of the red blood cell lysis treatment.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems, the utility model provides a full-automatic sample pretreatment device, which is used for pretreating a sample to be detected and comprises a sample placing module, a reagent supplying module, a liquid transferring module and a control module;
the sample placing module is provided with a test tube fixing device; the test tube fixing device is used for fixing a test tube containing a sample to be detected;
the reagent supply module comprises a sample pre-treatment unit; the sample pretreatment unit is used for placing a reagent bottle containing reagents required by sample pretreatment;
the liquid transfer module is used for transferring reagents required by sample pretreatment in the reagent bottle to a test tube containing a sample to be detected;
the control module is used for controlling the liquid transferring module, and further controlling the addition amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured.
In one embodiment of the present invention, the test tube fixing device is a test tube holder; the test tube clamp is used for clamping a test tube filled with a sample to be tested.
In one embodiment of the present invention, the sample pretreatment unit is provided with a reagent bottle cup holder; the reagent bottle cup frame is used for placing reagent bottles containing reagents required by sample pretreatment.
In one embodiment of the utility model, the pipetting module comprises a first flow control valve, a first pipetting pump and a liquid outlet head; three inlets and outlets of the first flow control valve are respectively communicated with the first liquid transfer pump, the liquid outlet head and the reagent bottle; the outlet of the liquid outlet head is aligned with the pipe orifice of the test tube containing the sample to be detected; under the driving of the first liquid transfer pump, reagents required by sample pretreatment in the reagent bottle are transferred from the reagent bottle to the first liquid transfer pump through the first flow control valve, then transferred from the first liquid transfer pump to the liquid outlet head through the first flow control valve, and finally flow into a test tube containing a sample to be detected from the liquid outlet head; the control module controls the pumping amount of the first liquid transferring pump so as to control the addition amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured.
In one embodiment of the utility model, the reagent supply module further comprises a washing unit; the cleaning unit is used for placing a cleaning bottle containing reagents required for cleaning.
In one embodiment of the utility model, the cleaning unit is provided with a cleaning bottle cup holder; the cleaning bottle cup holder is used for placing a cleaning bottle containing reagents required for cleaning.
In one embodiment of the utility model, the pipetting module comprises a first flow control valve, a second flow control valve, a first pipetting pump, a second pipetting pump and a liquid outlet head; two inlets and outlets of the first flow control valve are respectively communicated with the first liquid transfer pump and the reagent bottle; three inlets and outlets of the second flow control valve are respectively communicated with the cleaning bottle, the liquid outlet head and the rest inlet and outlet of the first flow control valve; the second liquid-moving pump is arranged between the cleaning bottle and the second flow control valve; the outlet of the liquid outlet head is aligned with the pipe orifice of the test tube containing the sample to be detected; under the driving of the first liquid transfer pump, reagents required by sample pretreatment in a reagent bottle are transferred from the reagent bottle to the first liquid transfer pump through the first flow control valve, then transferred from the first liquid transfer pump to the second flow control valve through the first flow control valve, then flow into the liquid outlet head from the second flow control valve, and finally flow into a test tube containing a sample to be detected from the liquid outlet head; under the driving of the second liquid transfer pump, reagents required by cleaning in the cleaning bottle are transferred to the liquid outlet head through the second flow control valve and then flow out of the liquid outlet head; the control module controls the adding amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured by controlling the pumping amount of the first liquid transferring pump, and controls the outflow amount of the reagent required by cleaning by controlling the pumping amount of the second liquid transferring pump.
In one embodiment of the present invention, the sample pretreatment apparatus further comprises a waste liquid collection module; the waste liquid collection module comprises a waste liquid barrel; the waste liquid barrel is used for collecting reagents required by sample pretreatment or cleaning and flowing out of the liquid outlet.
In one embodiment of the present invention, the waste liquid collecting module further comprises a guide rail and a moving frame; the moving frame is erected on the guide rail; the waste liquid barrel is placed on the movable frame; along with the sliding of the movable frame on the guide rail, the waste liquid barrel is displaced to the outlet below the liquid outlet head, so that the reagent required for sample pretreatment or the reagent required for cleaning flowing out of the liquid outlet flows into the waste liquid barrel.
In one embodiment of the present invention, the waste liquid collecting module further comprises a stopper; the limiting block is used for limiting the sliding range of the moving frame on the guide rail.
In one embodiment of the utility model, the waste collection module further comprises a position sensor; the position sensor is used for detecting the position of the waste liquid barrel.
In an embodiment of the present invention, the sample pretreatment apparatus further includes a blending module; the blending module comprises a blending device; the blending device is used for uniformly mixing the sample to be detected with the reagent required by the sample pretreatment; the control module controls the opening and closing of the blending device, so that the mixing time between the sample to be detected and the reagent required by the sample pretreatment is controlled.
In one embodiment of the utility model, the blending device comprises a blending motor, a motor connector, an eccentric wheel and a blending seat; one end of the motor connector is connected with an output shaft of the blending motor, and the other end of the motor connector is connected with the eccentric wheel; a bearing is arranged on the eccentric wheel; the mixing seat is arranged below the test tube fixing device and is contacted with the bottom of the test tube containing the sample to be detected; the mixing seat is provided with an installation groove; the mounting groove is sleeved on a bearing outer ring of the eccentric wheel; under the drive of a mixing motor, the motor connector drives the eccentric wheel to rotate, so as to drive the mixing seat to do circular motion, and finally, the sample to be detected in the test tube is driven to oscillate; the control module controls the mixing motor to be turned on and turned off so as to control the mixing time between the sample to be detected and the reagent required by the pretreatment of the sample, and controls the mixing speed between the sample to be detected and the reagent required by the pretreatment of the sample by controlling the rotating speed of the mixing motor.
In one embodiment of the present invention, the blending device further comprises a speed sensor; the speed sensor is used for detecting the rotating speed of the blending motor.
In an embodiment of the present invention, the control terminal of the control module is a display screen.
In one embodiment of the present invention, the sample pretreatment apparatus further comprises a housing; the display screen is arranged on the shell.
In one embodiment of the present invention, the sample pretreatment apparatus further comprises a treatment chamber; the sample placing module and the blending module are both arranged in the processing cabin; a position sensor is arranged at the cabin door of the processing cabin; the speed sensor is used for detecting the opening and closing of the cabin door; if the hatch door is in an open state, the control module stops the operation of the sample pretreatment device and displays a warning on the display screen.
In one embodiment of the present invention, the control module is loaded with an intelligent computing module; the intelligent calculation module is used for calculating the residual amount of liquid in the reagent bottle and/or the cleaning bottle; if the residual amount of the liquid in the reagent bottle and/or the cleaning bottle is insufficient, the control module displays a warning at the display screen.
In one embodiment of the utility model, the control module is loaded with an equipment maintenance module; when the sample pretreatment device is started, the control module can display a device maintenance interface at the display screen to prompt the user to check the residual quantity of the liquid in the reagent bottle and/or the cleaning bottle, check whether the liquid in the reagent bottle and/or the cleaning bottle is correct or not, and replace and supplement the required reagent or cleaning liquid when necessary.
In one embodiment of the present invention, the first flow control valve is a two-position three-way solenoid valve.
In one embodiment of the present invention, the second flow control valve is a two-position three-way solenoid valve.
In one embodiment of the utility model, the first pipetting pump is a plunger pump.
In one embodiment of the utility model, the second fluid transfer pump is a diaphragm pump.
The utility model also provides a sample pretreatment method, which is used for pretreating a sample to be detected by using the sample pretreatment device.
The utility model also provides the application of the sample pretreatment device or the method in pretreatment of a sample to be detected.
The technical scheme of the utility model has the following advantages:
the utility model provides a full-automatic sample pretreatment device, which comprises a sample placing module, a reagent supply module, a liquid transferring module and a control module, wherein the sample placing module is provided with a test tube fixing device, the test tube fixing device is used for fixing a test tube containing a sample to be detected, the reagent supply module comprises a sample pretreatment unit, the sample pretreatment unit is used for placing a reagent bottle containing a reagent required for sample pretreatment, the liquid transferring module is used for transferring the reagent required for sample pretreatment in the reagent bottle to the test tube containing the sample to be detected, and the control module is used for controlling the liquid transferring module so as to further control the addition amount of the reagent required for sample pretreatment in the test tube containing the sample to be detected; the full-automatic sample pretreatment device is used for pretreating cells to be detected, so that manual intervention can be reduced, the full-automatic operation of the pretreatment process of the samples to be detected can be realized only by preparing required cell treatment reagents in advance and setting different preset sample adding amounts of various cell treatment reagents in advance according to different treatment requirements on the cells to be detected, and the full-automatic sample pretreatment device has the characteristics of high liquid adding precision, controllable liquid adding amount and liquid adding time, simple operation steps, low labor cost, high treatment speed, high operation stability and low operation error rate; compared with the traditional manual operation, the full-automatic sample pretreatment device can complete the batch pretreatment process of the samples to be detected on one device, thereby obviously improving the batch treatment efficiency of the cells to be detected; the fully automatic sample pretreatment device can change the cell treatment reagent of the cells to be detected and adjust the preset sample adding amount of the cell treatment reagent according to different treatment requirements on the cells to be detected, and has a wide application range (for example, a whole blood sample containing red blood cells is subjected to red blood cell lysis treatment in turn by using three reagents of an A solution, a B solution and a C solution of EryLyse CA so as to be used for white blood cell flow detection).
Furthermore, the full-automatic sample pretreatment device also has a self-cleaning function, the self-cleaning function can realize the self-cleaning of each pipeline in the full-automatic sample pretreatment device, the operation is simple and convenient, the automation degree is higher, the operation steps of the sample pretreatment can be further simplified, and the labor cost of the sample pretreatment can be reduced.
Further, full-automatic sample pretreatment device is equipped with the waste liquid treatment module, the required reagent of sample pretreatment or washing that the waste liquid treatment module can collect from the liquid outlet outflow has further simplified the operating procedure of sample pretreatment and has reduced the human cost of sample pretreatment.
Further, full-automatic sample pretreatment device is equipped with the mixing module, the mixing module can be according to predetermineeing mixing time and mixing speed, and the eccentric mechanism through reagent socle portion drives the reagent pipe and is circular motion for the required reagent of each cell pretreatment is fully mixed with the sample that awaits measuring in the reagent pipe, has the controllable advantage of mixing dynamics, and is easy and simple to handle, and degree of automation is higher, helps further simplifying the operating procedure of sample pretreatment and reduces the human cost of sample pretreatment, and, the damage of mixing module to the sample is littleer, and the sample that obtains is more close before with the processing, helps improving the accuracy of follow-up detection.
Drawings
FIG. 1: the overall structure of one embodiment of the full-automatic sample pretreatment device is schematically shown.
FIG. 2: the reagent supply module and the pipetting module of the full-automatic sample pretreatment device are in a schematic overall structure.
FIG. 3: the front view of one embodiment of the sample placement module, the waste liquid collection module and the blending module of the full-automatic sample pretreatment device.
FIG. 4: the overall structure schematic diagram of one embodiment of the sample placing module and the waste liquid collecting module of the full-automatic sample pretreatment device.
FIG. 5: the overall structure schematic diagram of one embodiment of the waste liquid collecting module of the full-automatic sample pretreatment device.
FIG. 6: and a liquid path diagram (A liquid path) of a liquid transfer module of the full-automatic sample pretreatment device.
FIG. 7: and a liquid path diagram (B liquid path) of a liquid transfer module of the full-automatic sample pretreatment device.
FIG. 8: and a liquid path diagram (C liquid path) of a liquid transfer module of the fully automatic sample pretreatment device.
FIG. 9: a working flow chart of a pipetting module of the fully automatic sample pretreatment device (A liquid path injection mode).
FIG. 10: and (B) a work flow chart of a pipetting module of the fully automatic sample pretreatment apparatus (liquid path injection mode).
FIG. 11: a work flow chart of a pipetting module of the fully automatic sample pretreatment apparatus (C liquid path injection mode).
FIG. 12: and (B) a work flow chart of a pipetting module of the full-automatic sample pretreatment apparatus (liquid path cleaning mode).
In fig. 1 to 5, a sample placing module 1, a reagent supplying module 2, a pipetting module 3, a control module 4, a waste liquid collecting module 5, a mixing module 6, a housing 7, a display screen 8, a test tube 9, a sample pretreatment unit 10, a cleaning unit 11, a reagent bottle cup holder 12, a reagent bottle 13, a cleaning bottle cup holder 14, a cleaning bottle 15, a first flow control valve 16, a second flow control valve 17, a first liquid transferring pump 18, a second liquid transferring pump 19, a liquid outlet head 20, a waste liquid barrel 21, a guide rail 22, a moving frame 23, a limiting block 24, a position sensor 25, a mixing device 26, a mixing motor 27, a motor connector 28, an eccentric wheel 29, a mixing seat 30, a speed sensor 31, a bearing 32, an installation groove 33, a processing cabin 34, a cabin door 35 and a position sensor 36.
Detailed Description
The utility model will be further elucidated with reference to the embodiments and the drawings.
Example 1: a full-automatic sample pretreatment device (taking pretreatment of whole blood sample as an example)
As shown in fig. 1 to 5, the present embodiment provides a fully automatic sample pretreatment device, which is used for performing red blood cell lysis treatment on a whole blood sample (i.e., a sample to be detected) for white blood cell flow detection, wherein reagents required for the red blood cell lysis treatment are solutions a, B, and C of EryLyse CA;
the sample pretreatment device comprises a sample placing module 1, a reagent supply module 2, a liquid transfer module 3, a control module 4, a waste liquid collecting module 5, a uniform mixing module 6 and a shell 7;
the sample placing module 1 is provided with a test tube fixing device 8; the test tube fixing device 8 is a test tube clamp; the test tube clamp is used for clamping a test tube 9 filled with a sample to be tested;
the reagent supply module 2 comprises a sample pretreatment unit 10 and a cleaning unit 11; the sample pretreatment unit 10 is provided with three reagent bottle cup holders 12, and the three reagent bottle cup holders 12 are respectively used for holding three reagent bottles 13 containing liquid A, liquid B and liquid C; the cleaning unit 11 is provided with a cleaning bottle cup holder 14, and the cleaning bottle cup holder 14 is used for placing a cleaning bottle 15 filled with cleaning liquid;
the pipetting module 3 comprises three first flow control valves 16, one second flow control valve 17, three first pipetting pumps 18, one second pipetting pump 19 and three liquid outlet heads 20; the first flow control valve 16 is a two-position three-way electromagnetic valve, and the three two-position three-way electromagnetic valves are a No. 1 electromagnetic valve, a No. 2 electromagnetic valve and a No. 3 electromagnetic valve respectively; the second flow control valve 17 is a two-position three-way electromagnetic valve, and the two-position three-way electromagnetic valve is a No. 4 electromagnetic valve; the first liquid transfer pump 18 is a plunger pump, and the three plunger pumps are respectively an A plunger pump, a B plunger pump and a C plunger pump; the second liquid transfer pump 19 is a diaphragm pump; the three liquid outlet heads 20 are respectively an A liquid outlet head, a B liquid outlet head and a C liquid outlet head;
three inlets and outlets of the No. 1 electromagnetic valve are respectively communicated with the plunger pump A, the liquid outlet head A and the reagent bottle containing the liquid A; the outlet of the liquid outlet head A is aligned with the pipe orifice of the test tube filled with the sample to be detected; under the drive of the plunger pump A, liquid A in the reagent bottle is firstly transferred from the reagent bottle to the plunger pump A through the solenoid valve No. 1, then transferred from the plunger pump A to the liquid outlet head A through the solenoid valve No. 1, and finally flows into a test tube containing a sample to be detected from the liquid outlet head A; the control module 4 controls the pumping amount of the plunger pump A to further control the adding amount of the liquid A in the test tube filled with the sample to be detected (a liquid path A, a liquid path diagram is shown in figure 6);
two inlets and outlets of the No. 2 electromagnetic valve are respectively communicated with the plunger pump B and a reagent bottle containing the liquid B; three inlets and outlets of the No. 4 electromagnetic valve are respectively communicated with the cleaning bottle, the liquid outlet head B and the rest inlet and outlet of the No. 2 electromagnetic valve; the diaphragm pump is arranged between the cleaning bottle and the No. 4 electromagnetic valve; the outlet of the liquid outlet head B is aligned to the pipe orifice of the test tube containing the sample to be tested; under the drive of the plunger pump B, liquid B in the reagent bottle is firstly transferred from the reagent bottle to the plunger pump B through the solenoid valve No. 2, then transferred from the plunger pump B to the solenoid valve No. 4 through the solenoid valve No. 2, then flows into the liquid outlet head B from the solenoid valve No. 4, and finally flows into a test tube containing a sample to be detected from the liquid outlet head B; under the driving of the diaphragm pump, the cleaning liquid in the cleaning bottle is firstly transferred to the liquid outlet head B through the No. 4 electromagnetic valve and then flows out of the liquid outlet head B; the control module 4 controls the pumping amount of the plunger pump B to further control the addition amount of the liquid B in the test tube filled with the sample to be detected, and controls the pumping amount of the diaphragm pump to further control the outflow amount of the cleaning liquid (a liquid path B, a liquid path diagram of which is shown in figure 7);
three inlets and outlets of the No. 3 electromagnetic valve are respectively communicated with the C plunger pump, the C liquid outlet head and a reagent bottle containing the C liquid; the outlet of the liquid outlet head C is aligned to the pipe orifice of the test tube containing the sample to be tested; under the drive of the plunger pump C, the liquid C in the reagent bottle is firstly transferred from the reagent bottle to the plunger pump C through the solenoid valve No. 3, then transferred from the plunger pump C to the liquid outlet head C through the solenoid valve No. 3, and finally flows into a test tube containing a sample to be detected from the liquid outlet head C; the control module 4 controls the pumping amount of the plunger pump C to further control the adding amount of the liquid C in the test tube filled with the sample to be detected (a liquid path C, and a liquid path diagram is shown in figure 8);
the waste liquid collecting module 5 comprises a waste liquid barrel 21, a guide rail 22, a moving frame 23, a limiting block 24 and a position sensor 25; the moving frame 23 is erected on the guide rail 22; the waste liquid barrel 21 is placed on the moving rack 23; along with the sliding of the movable frame 23 on the guide rail 22, the waste liquid barrel 21 is displaced to the lower part of the outlets of the liquid outlet heads A, B and C, so that the liquid A, B and C or the cleaning liquid flowing out of the liquid outlet heads A, B and C flows into the waste liquid barrel 21; the limiting block 24 is used for limiting the sliding range of the moving frame 23 on the guide rail 22; the position sensor 25 is used for detecting the position of the waste liquid barrel 21;
the blending module 6 comprises a blending device 26; the blending device 26 comprises a blending motor 27, a motor connector 28, an eccentric wheel 29, a blending seat 30 and a speed sensor 31; one end of the motor connector 28 is connected with an output shaft of the blending motor 27, and the other end of the motor connector is connected with an eccentric wheel 29; a bearing 32 is arranged on the eccentric wheel 29; the mixing seat 30 is arranged below the test tube fixing device 8 so as to be contacted with the bottom of the test tube 9 containing the sample to be measured; the mixing seat 30 is provided with an installation groove 33; the mounting groove 33 is sleeved on the outer ring of the bearing 32 of the eccentric wheel 29; under the driving of the blending motor 27, the motor connector 28 drives the eccentric wheel 29 to rotate, further drives the blending seat 30 to do circular motion, and finally drives the sample to be tested in the test tube 9 to oscillate, so that the liquid A, the liquid B and the liquid C are sequentially and uniformly mixed with the sample to be tested; the control module 4 controls the opening and closing of the blending motor 27 to control the mixing time between the sample to be detected and the reagent required by the sample pretreatment, and controls the mixing speed between the sample to be detected and the reagent required by the sample pretreatment by controlling the rotating speed of the blending motor 27; the speed sensor 31 is used for detecting the rotating speed of the blending motor 27;
the control terminal of the control module 4 is a display screen 8; the display screen 8 is arranged on the shell 7; the sample pre-processing apparatus further comprises a processing compartment 34; the sample placing module 1 and the blending module 6 are both arranged in the processing cabin 34; a position sensor 36 is arranged at a cabin door 35 of the processing cabin 34; the speed sensor 36 is used for detecting the opening and closing of the cabin door 35; if the hatch 35 is in the open state, the control module 4 will stop the operation of the sample pretreatment device and display a warning on the display screen 8; the control module 4 is loaded with an intelligent computing module; the intelligent calculation module is used for calculating the residual amount of liquid in the reagent bottle 13 and/or the cleaning bottle 15; if the residual amount of the liquid in the reagent bottle 13 and/or the cleaning bottle 15 is insufficient, the control module 4 displays a warning at the display screen; the control module 4 is loaded with an equipment maintenance module; when the sample pretreatment device is started, the control module 4 displays a device maintenance interface at the display screen 8, prompts to check the residual amount of the liquid in the reagent bottle 13 and/or the cleaning bottle 15, checks whether the liquid in the reagent bottle 13 and/or the cleaning bottle 15 is correct, and replaces or supplements the required reagent or cleaning liquid when necessary.
The working flow of the sample pretreatment device is as follows:
1. early preparation state
When the sample pretreatment device is used for the first time, the pretreatment of a sample to be detected can be influenced when no liquid or the liquid is overdue in a pipeline, all pipelines and a plunger pump cavity are required to be cleaned and filled, and the operation steps are as follows:
the sliding moving frame moves the waste liquid barrel to the positions below the outlets of the liquid outlet heads A, B and C;
the No. 1 electromagnetic valve is not electrified, the A plunger pump is communicated with the A reagent bottle, and the A plunger pump works to suck the A liquid into the cavity of the A plunger pump; the No. 1 electromagnetic valve is electrified, the channel is switched, the A plunger pump is communicated with the A liquid outlet head, and the A plunger pump pushes the A liquid in the cavity to the A liquid outlet head and then flows into the waste liquid barrel from the A liquid outlet head (the working process can be shown in figure 9);
the No. 2 and No. 4 electromagnetic valves are not electrified, the B plunger pump is communicated with the B reagent bottle, and the B plunger pump works to suck the B liquid into a cavity of the B plunger pump; the No. 2 electromagnetic valve is electrified, the No. 4 electromagnetic valve is not electrified, the B plunger pump is communicated with the B liquid outlet head, and the B plunger pump pushes the B liquid in the cavity to the B liquid outlet head and then flows into the waste liquid barrel from the B liquid outlet head (the working process can be shown in figure 10);
the No. 3 electromagnetic valve is not electrified, the C plunger pump is communicated with the C reagent bottle, and the C plunger pump works to suck the C liquid into the cavity of the C plunger pump; the No. 3 electromagnetic valve is powered on, the channel is switched, the C plunger pump is communicated with the C liquid outlet head, and C liquid in the cavity is pushed to the C liquid outlet head by the operation of the C plunger pump and then flows into the waste liquid barrel from the C liquid outlet head (the working process can be shown in figure 11);
the sliding moving frame makes the waste liquid barrel leave the outlet lower parts of the liquid outlet heads A, B and C.
2. Pre-processing state of sample
Pushing a test tube filled with a sample to be tested into the test tube clamp, and enabling test tube ports of the test tube to be positioned below outlets of a liquid outlet head A, a liquid outlet head B and a liquid outlet head C;
the method comprises the following steps of setting the addition amounts of liquid A, liquid B and liquid C in a sample to be detected in a control system, and setting the mixing speed and mixing time of the liquid A, the liquid B and the liquid C with the sample to be detected in the control system;
the No. 1 electromagnetic valve is not electrified, the A plunger pump is communicated with the A reagent bottle, and the A plunger pump works to suck a set amount of A liquid into the cavity of the A plunger pump; the No. 1 electromagnetic valve is electrified, the channel is switched, the A plunger pump is communicated with the A liquid outlet head, and after the A plunger pump works to push the A liquid in the cavity to the A liquid outlet head, the A liquid flows into a test tube containing a sample to be detected from the A liquid outlet head (the working process can be shown in figure 9); after the liquid A is added, starting a mixing motor, and mixing the liquid A and a sample to be detected at a set speed for a set time;
the No. 2 and No. 4 electromagnetic valves are not electrified, the B plunger pump is communicated with the B reagent bottle, and the B plunger pump works to suck a set amount of B liquid into a cavity of the B plunger pump; the No. 2 electromagnetic valve is electrified, the No. 4 electromagnetic valve is not electrified, the B plunger pump is communicated with the B liquid outlet head, and after the B plunger pump works to push the B liquid in the cavity to the B liquid outlet head, the B liquid flows into a test tube filled with a sample to be detected from the B liquid outlet head (the working process can be seen in figure 10); after the liquid B is added, starting a mixing motor, and mixing the liquid B and the sample to be detected at a set speed for a set time;
the No. 3 electromagnetic valve is not electrified, the C plunger pump is communicated with the C reagent bottle, and the C plunger pump works to suck a set amount of C liquid into the cavity of the C plunger pump; the No. 3 electromagnetic valve is electrified, the channel is switched, the C plunger pump is communicated with the C liquid outlet head, and after the C plunger pump works to push the C liquid in the cavity to the C liquid outlet head, the C liquid flows into a test tube containing a sample to be detected from the C liquid outlet head (the working process can be shown in figure 11); after the liquid C is added, starting a mixing motor, and mixing the liquid C and the sample to be detected at a set speed for a set time;
and clamping the test tube containing the sample to be detected from the test tube clamp.
3. Late stage cleaning state
The sliding moving frame moves the waste liquid barrel to the positions below the outlets of the liquid outlet heads A, B and C;
the No. 2 electromagnetic valve is not electrified, the No. 4 electromagnetic valve is electrified, the diaphragm pump is communicated with the cleaning solution, the cleaning solution is pushed to the liquid outlet head B by the diaphragm pump, and then flows into the waste liquid barrel from the liquid outlet head B (the working process can be shown in figure 12);
if the liquid A and the liquid C are also cleaned, the reagent bottle may be replaced with a cleaning bottle, or the line may be inserted into a cleaning bottle of the liquid B.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the utility model as defined in the appended claims.
Claims (10)
1. The sample pretreatment device is characterized by comprising a sample placing module, a reagent supply module, a pipetting module and a control module;
the sample placing module is provided with a test tube fixing device; the test tube fixing device is used for fixing a test tube containing a sample to be detected;
the reagent supply module comprises a sample pre-treatment unit; the sample pretreatment unit is used for placing a reagent bottle containing reagents required by sample pretreatment;
the liquid transfer module is used for transferring reagents required by sample pretreatment in the reagent bottle to a test tube containing a sample to be detected;
the control module is used for controlling the liquid transferring module, and further controlling the addition amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured.
2. The sample pretreatment apparatus according to claim 1, wherein the pipetting module includes a first flow control valve, a first pipetting pump, and a liquid outlet head; three inlets and outlets of the first flow control valve are respectively communicated with the first liquid transfer pump, the liquid outlet head and the reagent bottle; the outlet of the liquid outlet head is aligned with the pipe orifice of the test tube containing the sample to be detected; under the driving of the first liquid transfer pump, reagents required by sample pretreatment in the reagent bottle are transferred from the reagent bottle to the first liquid transfer pump through the first flow control valve, then transferred from the first liquid transfer pump to the liquid outlet head through the first flow control valve, and finally flow into a test tube containing a sample to be detected from the liquid outlet head; the control module controls the pumping amount of the first liquid transferring pump so as to control the addition amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured.
3. The sample pretreatment apparatus of claim 1, wherein the reagent supply module further comprises a washing unit; the cleaning unit is used for placing a cleaning bottle containing reagents required for cleaning.
4. The sample pretreatment apparatus according to claim 3, wherein the pipetting module includes a first flow control valve, a second flow control valve, a first pipetting pump, a second pipetting pump, and a liquid outlet head; two inlets and outlets of the first flow control valve are respectively communicated with the first liquid transfer pump and the reagent bottle; three inlets and outlets of the second flow control valve are respectively communicated with the cleaning bottle, the liquid outlet head and the rest inlet and outlet of the first flow control valve; the second liquid transfer pump is arranged between the cleaning bottle and the second flow control valve; the outlet of the liquid outlet head is aligned with the pipe orifice of the test tube containing the sample to be detected; under the driving of the first liquid transfer pump, reagents required by sample pretreatment in a reagent bottle are transferred from the reagent bottle to the first liquid transfer pump through the first flow control valve, then transferred from the first liquid transfer pump to the second flow control valve through the first flow control valve, then flow into the liquid outlet head from the second flow control valve, and finally flow into a test tube containing a sample to be detected from the liquid outlet head; under the driving of the second liquid transfer pump, reagents required by cleaning in the cleaning bottle are transferred to the liquid outlet head through the second flow control valve and then flow out of the liquid outlet head; the control module controls the adding amount of the reagent required by the sample pretreatment in the test tube containing the sample to be measured by controlling the pumping amount of the first liquid transferring pump, and controls the outflow amount of the reagent required by cleaning by controlling the pumping amount of the second liquid transferring pump.
5. The sample pretreatment apparatus according to claim 2 or 4, further comprising a waste liquid collection module; the waste liquid collection module comprises a waste liquid barrel; the waste liquid barrel is used for collecting reagents required by sample pretreatment or cleaning and flowing out of the liquid outlet.
6. The sample pretreatment apparatus of claim 5, wherein the waste collection module further comprises a guide rail and a movable rack; the movable frame is erected on the guide rail; the waste liquid barrel is placed on the movable frame; along with the sliding of the movable frame on the guide rail, the waste liquid barrel is displaced to the outlet below the liquid outlet head, so that the reagent required for sample pretreatment or the reagent required for cleaning flowing out of the liquid outlet flows into the waste liquid barrel.
7. The sample pretreatment apparatus of claim 2 or 4, further comprising a blending module; the blending module comprises a blending device; the blending device is used for uniformly mixing the sample to be detected with the reagent required by the sample pretreatment; the control module controls the opening and closing of the blending device, so that the mixing time between the sample to be detected and the reagent required by the sample pretreatment is controlled.
8. The sample pretreatment device according to claim 7, wherein the mixing device comprises a mixing motor, a motor connector, an eccentric wheel and a mixing base; one end of the motor connector is connected with an output shaft of the blending motor, and the other end of the motor connector is connected with the eccentric wheel; a bearing is arranged on the eccentric wheel; the mixing seat is arranged below the test tube fixing device and is contacted with the bottom of the test tube containing the sample to be detected; the mixing seat is provided with an installation groove; the mounting groove is sleeved on the bearing outer ring of the eccentric wheel; under the drive of a mixing motor, the motor connector drives the eccentric wheel to rotate, so as to drive the mixing seat to do circular motion, and finally, the sample to be detected in the test tube is driven to oscillate; the control module controls the opening and closing of the mixing motor to further control the mixing time between the sample to be detected and the reagent required for sample pretreatment, and controls the mixing speed between the sample to be detected and the reagent required for sample pretreatment by controlling the rotating speed of the mixing motor.
9. The sample pretreatment apparatus of claim 8, wherein the intermixing device further comprises a speed sensor; the speed sensor is used for detecting the rotating speed of the blending motor.
10. The sample pretreatment apparatus according to claim 2 or 4, wherein a control terminal of the control module is a display screen.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115253682A (en) * | 2022-07-29 | 2022-11-01 | 烟台至公生物医药科技有限公司 | Bladder cancer cell capturing device and capturing method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115253682A (en) * | 2022-07-29 | 2022-11-01 | 烟台至公生物医药科技有限公司 | Bladder cancer cell capturing device and capturing method |
| CN115253682B (en) * | 2022-07-29 | 2024-02-02 | 烟台至公生物医药科技有限公司 | Bladder cancer cell capturing device and capturing method |
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