CN115253682A - Bladder cancer cell capturing device and capturing method - Google Patents
Bladder cancer cell capturing device and capturing method Download PDFInfo
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- CN115253682A CN115253682A CN202210906027.1A CN202210906027A CN115253682A CN 115253682 A CN115253682 A CN 115253682A CN 202210906027 A CN202210906027 A CN 202210906027A CN 115253682 A CN115253682 A CN 115253682A
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- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 title claims abstract description 145
- 206010005003 Bladder cancer Diseases 0.000 title claims abstract description 144
- 201000005112 urinary bladder cancer Diseases 0.000 title claims abstract description 144
- 238000000034 method Methods 0.000 title claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 382
- 239000012528 membrane Substances 0.000 claims abstract description 312
- 239000002699 waste material Substances 0.000 claims abstract description 6
- 239000013049 sediment Substances 0.000 claims description 45
- 230000002485 urinary effect Effects 0.000 claims description 31
- 239000007853 buffer solution Substances 0.000 claims description 29
- 239000003085 diluting agent Substances 0.000 claims description 22
- 210000002700 urine Anatomy 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 10
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 110
- 239000000523 sample Substances 0.000 description 21
- 208000006750 hematuria Diseases 0.000 description 6
- 238000007865 diluting Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 238000001471 micro-filtration Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
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- 238000010586 diagram Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001575 pathological effect Effects 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 206010007027 Calculus urinary Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000009911 Urinary Calculi Diseases 0.000 description 1
- 206010064921 Urinary tract inflammation Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 208000029584 urinary system neoplasm Diseases 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D65/00—Accessories or auxiliary operations, in general, for separation processes or apparatus using semi-permeable membranes
- B01D65/02—Membrane cleaning or sterilisation ; Membrane regeneration
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
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- Chemical Kinetics & Catalysis (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
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Abstract
The application provides a bladder cancer cell capturing device and a capturing method, wherein the device comprises: a microporous filter membrane is arranged in the membrane changing filter; the filter switch assembly is connected with the membrane replacing filter and is used for opening/closing the membrane replacing filter; a filter membrane taking and placing device for taking and placing the microporous filter membrane; and the liquid in the second liquid storage pool flows into the first liquid storage pool through the second pump, and the liquid in the first liquid storage pool flows into the membrane replacing filter through the first pump, so that the microporous filter membrane captures the bladder cancer cells. The technical scheme provided by the application can reduce manual operation steps, simplify the difficulty in capturing the bladder cancer cells and improve the efficiency in capturing the bladder cancer cells. When bladder cancer cells are captured, only the microporous filter membrane needs to be replaced, the material cost is low, and the waste of resources is reduced.
Description
Technical Field
The application relates to the technical field of biomedical engineering, in particular to a bladder cancer cell capturing device and a capturing method.
Background
Bladder cancer is the most common neoplasm of the urinary system. The bladder cancer is latent in onset, and hematuria is one of clinical early symptoms of bladder cancer patients and comprises macroscopic hematuria and microscopic hematuria. However, hematuria also occurs under urinary tract inflammation and stone symptoms, and is not a specific symptom of bladder cancer. Due to the difference of bleeding amount, when the number of red blood cells in urine reaches a certain threshold value, the color of the urine is red by eye observation, for example, the color of a meat washing water sample or the color is light red, pink and the like, which can be called as naked eye hematuria. Bladder cancer typically develops in patients with bladder tumors that develop into relatively large tumor nodules as early-onset symptoms are not apparent, such that hematuria is observed. Therefore, timely diagnosis of bladder cancer is of great importance.
A commonly used diagnostic method for bladder cancer includes analysis of urine exfoliated cells, which can be identified and distinguished from normal cells by microscopic observation of urine exfoliated cells in the urinary sediment. However, when urine exfoliated cell examination is used, in order to ensure the success rate of the examination, three consecutive examinations are usually required.
The inventor finds that if bladder cancer cells can be captured from urinary sediment, bladder cancer can be quickly diagnosed, the problem that clinical patients of bladder cancer have no pathological samples or few pathological samples can be solved, and scientific reference basis can be provided for diagnosis and prognosis of clinicians. However, in the prior art, the capturing of the bladder cancer cells from the urinary sediment requires a special person to operate, and the capturing efficiency is relatively low. Therefore, a bladder cancer cell capturing device is highly desirable.
Disclosure of Invention
In view of this, the present application provides a bladder cancer cell capturing device and a capturing method, so as to solve the technical problem in the prior art that the efficiency of capturing bladder cancer cells from urinary sediments is low.
In a first aspect, the present application provides a bladder cancer cell capture device comprising:
the membrane replacing filter is internally provided with a microfiltration membrane, and the microfiltration membrane is used for capturing bladder cancer cells in liquid flowing through the membrane replacing filter;
the filter switch assembly is connected with the membrane replacing filter and is used for switching on or off the membrane replacing filter;
the filter membrane taking and placing device is used for taking and placing the microporous filter membrane;
the bottom of the first liquid storage tank is provided with a first liquid guide pipe, the first liquid guide pipe is connected with a liquid inlet of the membrane changing filter, liquid in the first liquid storage tank flows to the membrane changing filter through the first liquid guide pipe, and a first pump is arranged on the first liquid guide pipe; a second liquid guide pipe is arranged at the top of the first liquid storage tank, a second pump is arranged on the second liquid guide pipe, and liquid in a third liquid storage tank flows to the second liquid storage tank through the second liquid guide pipe;
the third liquid storage tank is arranged above the first liquid storage tank, a third liquid guide pipe and a fourth liquid guide pipe are arranged at the bottom of the third liquid storage tank, liquid in the third liquid storage tank flows to the first liquid storage tank through the third liquid guide pipe, liquid in the third liquid storage tank flows to the second liquid storage tank through the fourth liquid guide pipe, and a first valve is arranged on the third liquid guide pipe; a second valve is arranged on the fourth liquid guide pipe;
and the controller is connected with the filter membrane taking and placing assembly, the first pump, the second pump, the first valve and the second valve.
In a first embodiment of the first aspect, the filter switch assembly comprises:
the positioning bracket is fixedly connected with the lower part of the membrane changing filter, and the lower part of the membrane changing filter is a part where a liquid outlet of the membrane changing filter is located;
the shifting unit comprises a connecting support, a sliding block and a telescopic rod, the connecting support is connected with the positioning support, the sliding block is fixedly connected with the upper portion of the membrane replacing filter, the upper portion of the membrane replacing filter is the portion where the liquid inlet of the membrane replacing filter is located, a sliding groove is formed in the connecting support, the connecting support is connected with the sliding block in a sliding mode, the sliding block slides along the sliding groove, the sliding block is connected with the telescopic rod at the top of the connecting support, and the telescopic rod is connected with the controller.
In a second embodiment of the first aspect, the filter membrane retrieval device comprises a robotic arm for grasping a microfiltration membrane at a first location and placing the grasped microfiltration membrane at a second location.
In a third embodiment of the first aspect, the bladder cancer cell capturing device further comprises:
and the liquid inlet end of the fifth liquid guide pipe is connected with the liquid outlet of the membrane replacing filter, and the liquid at the liquid outlet of the membrane replacing filter flows to the waste liquid tank through the fifth liquid guide pipe.
In a fourth embodiment of the first aspect, the bladder cancer cell capturing device further comprises:
an oscillator connected with the second reservoir.
In a second aspect, according to the first aspect, the present application provides a bladder cancer cell capture method comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
In a third aspect, according to the first aspect, the present application provides a bladder cancer cell capture method comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a first valve, guiding the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
In a fourth aspect, according to a fourth embodiment of the first aspect, the present application provides a bladder cancer cell capture method comprising:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a second valve, leading the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
In a fifth aspect, according to a fourth embodiment of the first aspect, the present application provides a bladder cancer cell capture method comprising:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
opening a first valve, leading the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
In a sixth aspect, according to the first aspect, the present application provides a method of cleaning a bladder cancer cell capture device, comprising:
the filter switch component turns on the membrane replacing filter; a filter membrane taking and placing device takes out the microporous filter membrane in the membrane replacing filter; the filter switch component closes the membrane replacing filter;
putting the buffer solution into a third liquid storage tank;
opening a second valve, leading the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump.
The bladder cancer cell capturing device and the capturing method provided by the application have at least the following beneficial effects:
the technical scheme that this application provided gets through filter switch subassembly and filter membrane and puts the ware to realize that millipore filtration's automation loads and gets and put the flow, the setting of second catheter and second pump flows into first liquid storage tank with the sample liquid in the second liquid storage tank, and the setting of first catheter and first pump is with the sample liquid inflow in the first liquid storage tank and is traded membrane filter, so that the millipore filtration in the membrane filter that trades catches bladder cancer cell. When the bladder cancer cell capturing device provided by the application captures bladder cancer cells, manual operation steps are reduced, the difficulty in capturing the bladder cancer cells is simplified, and the capturing efficiency of the bladder cancer cells is improved. The third reservoir may be arranged to wash the fluid channel of the capturing device with a buffer fluid in the third reservoir. Due to the arrangement of the membrane replacement filter, only the microporous filter membrane needs to be replaced when bladder cancer cells are captured, the material cost is low, and the waste of resources is reduced.
According to the technical scheme provided by the application, the bladder cancer cells in the urinary sediment diluent can be captured by using the bladder cancer cell capturing device provided by the invention. After the microporous filter membrane is placed in the membrane replacing filter, the urinary sediment diluent flows into the first liquid storage tank from the second liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank so as to capture bladder cancer cells through the microporous filter membrane in the membrane replacing filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to the technical scheme provided by the application, the bladder cancer cells in the urinary sediment diluent can be captured by using the bladder cancer cell capturing device provided by the invention. After the microporous filter membrane is placed in the membrane replacing filter, the buffer solution is firstly led into the first liquid storage tank from the third liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank, so that bubbles on the microporous filter membrane in the membrane replacing filter are eliminated, and the capturing efficiency of bladder cancer cells is improved. And then the urinary sediment diluent flows into the first liquid storage tank from the second liquid storage tank and flows into the membrane changing filter from the first liquid storage tank so as to capture the bladder cancer cells through a microporous filter membrane in the membrane changing filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to the technical scheme provided by the application, the bladder cancer cell capturing device comprising the oscillator can capture bladder cancer cells in undiluted urine sediment liquid. The buffer solution in the third liquid storage tank is introduced into the second liquid storage tank, and the undiluted urinary sediment liquid in the second liquid storage tank is diluted and uniformly mixed through the work of the oscillator, so that the corresponding urinary sediment diluent is obtained. After the microporous filter membrane is placed in the membrane replacement filter, diluting and uniformly mixing to obtain corresponding urinary sediment diluent, flowing into the first liquid storage tank from the second liquid storage tank, and flowing into the membrane replacement filter from the first liquid storage tank so as to capture bladder cancer cells through the microporous filter membrane in the membrane replacement filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to the technical scheme provided by the application, the bladder cancer cell capturing device comprising the oscillator can capture bladder cancer cells in undiluted urine sediment liquid. The buffer solution in the third liquid storage tank is introduced into the second liquid storage tank, and the undiluted urinary sediment liquid in the second liquid storage tank is diluted and uniformly mixed through the work of the oscillator, so that the corresponding urinary sediment diluent is obtained. After the microporous filter membrane is placed in the membrane replacing filter, the buffer solution is firstly led into the first liquid storage tank from the third liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank, so that bubbles on the microporous filter membrane in the membrane replacing filter are eliminated, and the capturing efficiency of bladder cancer cells is improved. And then the corresponding urinary sediment diluent obtained after dilution and uniform mixing flows into the first liquid storage tank from the second liquid storage tank and flows into the membrane changing filter from the first liquid storage tank so as to capture bladder cancer cells through a microporous filter membrane in the membrane changing filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to the technical scheme provided by the application, the bladder cancer cell capturing device provided by the application is utilized, and the buffer solution in the third liquid storage tank is introduced into the second liquid storage tank so as to clean the second liquid storage tank; the buffer solution in the second liquid storage tank flows into the first liquid storage tank so as to clean the first liquid storage tank; and (4) enabling the buffer solution in the first liquid storage tank to flow into the membrane replacing filter so as to clean the interior of the membrane replacing filter. The cleaning device can clean the interiors of a liquid storage tank, a liquid guide pipe and a membrane replacing filter in the bladder cancer cell capturing device, so that the bladder cancer cell capturing device can be repeatedly used. Further improves the bladder cancer capturing efficiency of the bladder cancer cell capturing device provided by the application.
Drawings
In order to more clearly illustrate the detailed description of the present application or the technical solutions in the prior art, the drawings that are needed in the detailed description of the present application or the technical solutions in the prior art will be briefly described below. It should be apparent that the drawings in the following description are embodiments of the present application and that other drawings may be derived from those drawings by a person of ordinary skill in the art without inventive step. It should be noted that the drawings in the following description are illustrative and should not be construed as limiting the application in any way, and in that:
FIG. 1 shows a schematic structural diagram of a bladder cancer cell capturing device according to one embodiment of the present application;
fig. 2 shows a schematic structural diagram of a membrane replacement filter and a filter switch assembly according to an embodiment of the present application.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions of the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any inventive step based on the embodiments in the present application, are within the scope of the present application.
In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present application. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present application, it should be noted that, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; the two elements may be directly connected or indirectly connected through an intermediate medium, or may be communicated with each other inside the two elements, or may be wirelessly connected or wired connected. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
In addition, the technical features mentioned in the different embodiments of the present application described below can be combined with each other as long as they do not conflict with each other.
Although the processes described below include multiple operations that occur in a particular order, it should be clearly understood that the processes may include more or fewer operations that are performed sequentially or in parallel.
Example 1
The present embodiment provides a bladder cancer cell capturing apparatus, as shown in fig. 1, including:
the membrane replacing filter 1 is internally provided with a microporous filter membrane 3 which is used for capturing bladder cancer cells in liquid flowing through the membrane replacing filter. Specifically, the membrane-changing filter comprises an upper part and a lower part, and the upper part and the lower part can be in threaded connection or sliding plug-in connection. The microporous filter membrane can comprise a polycarbonate membrane and a nickel microporous membrane. For example, the thickness of the polycarbonate membrane is 10 μm, the diameter of the membrane is adapted to the diameter of the membrane-changing filter, the pore size is 8 μm, and the number of pores is about 106And (4) respectively.
And the filter switch assembly 2 is connected with the membrane replacing filter and is used for switching on or off the membrane replacing filter. Specifically, the filter switch assembly may include a first portion and a second portion, wherein the first portion is configured to be fixedly connected to a lower portion of the membrane changing filter, the second portion is configured to be fixedly connected to an upper portion of the membrane changing filter, and the first portion and the second portion are configured to cooperate with each other to separate or connect the upper portion of the membrane changing filter from or to the lower portion of the membrane changing filter. For example, if the upper and lower portions of the membrane replacement filter are threadedly coupled, the first portion of the filter switch assembly may be fixed, and the second portion may be threadedly raised or lowered with respect to the central axis of the first portion, thereby coupling or decoupling the upper portion of the membrane replacement filter coupled to the second portion to or from the lower portion of the membrane replacement filter fixedly mounted to the first portion. If the upper part and the lower part of the membrane changing filter are connected in a sliding and plugging way, the second part ascends or descends relative to the central axis of the first part, and the upper part of the membrane changing filter connected with the second part can be connected with or separated from the lower part of the membrane changing filter fixedly arranged on the first part without following a thread track.
In one embodiment, referring to fig. 2, the first portion of the filter switch assembly may be a positioning bracket 21 fixedly connected to the lower portion 12 of the membrane-changing filter, which is the portion of the membrane-changing filter where the liquid outlet is located. The filter switch component further comprises a shifting unit, the shifting unit comprises a connecting support 22, a sliding block 23 and a telescopic rod 24, the connecting support is connected with the positioning support, the sliding block is fixedly connected with the upper portion of the membrane replacing filter, the upper portion 11 of the membrane replacing filter is the portion where the liquid inlet of the membrane replacing filter is located, a sliding groove is formed in the connecting support, the connecting support is connected with the sliding block in a sliding mode, the sliding block slides along the sliding groove direction, the sliding block is connected with the telescopic rod at the top of the connecting support, and the telescopic rod is connected with the controller. It should be noted that, here, the sliding block corresponds to the second portion of the filter switch assembly described above, and the sliding block is driven by the telescopic rod to approach to and separate from the positioning bracket, that is, the upper portion and the lower portion of the membrane replacement filter can be connected and separated in a plugging manner.
And the filter membrane taking and placing device is used for taking and placing the microporous filter membrane. Specifically, the filter membrane taking and placing device can be a mechanical arm, the mechanical arm is provided with a clamping unit and used for grabbing the microporous filter membrane located at the first position, and the mechanical arm can place the grabbed microporous filter membrane at the second position. In practical application, the used microporous filter membrane is generally clamped and placed in the membrane replacement filter, and the microporous filter membrane in the membrane replacement filter, which captures bladder cancer cells, is clamped in the microporous filter membrane storage box.
The liquid in the first liquid storage tank flows to the membrane changing filter through the first liquid guide pipe, and the first liquid guide pipe is provided with a first pump; the top of the first liquid storage tank is provided with a second liquid guide pipe 8, the second liquid guide pipe is provided with a second pump, and liquid in the third liquid storage tank flows to the second liquid storage tank 5 through the second liquid guide pipe.
A third liquid storage tank 6 arranged above the first liquid storage tank, wherein the bottom of the third liquid storage tank is provided with a third liquid guide pipe 9 and a fourth liquid guide pipe 10, liquid in the third liquid storage tank flows to the first liquid storage tank through the third liquid guide pipe, liquid in the third liquid storage tank flows to the second liquid storage tank through the fourth liquid guide pipe, and the third liquid guide pipe is provided with a first valve; the fourth liquid guide pipe is provided with a second valve.
And the controller is connected with the filter membrane taking and placing assembly, the first pump, the second pump, the first valve and the second valve. Specifically, the controller may be connected to a memory, and the memory may store corresponding control logic information in advance. The control logic information comprises opening and closing control logic information of the filter membrane taking and placing assembly, the first pump, the second pump, the first valve and the second valve. It should be noted that the present embodiment mainly describes the hardware structure of the bladder cancer cell capturing device, and the corresponding control logic of the controller can fully adopt the prior art.
In this embodiment, specifically, the third liquid storage tank is used for storing a buffer solution, and the second liquid storage tank is used for storing a sample solution to be captured of bladder cancer cells. The buffer solution in the third liquid storage tank can be used for eliminating bubbles of the microporous filter membrane and cleaning the capture device.
In this embodiment, get the ware through filter switch subassembly and filter membrane to realize the automatic loading of millipore filtration membrane and get the process of putting, the setting of second catheter and second pump is with the sample liquid inflow in the second liquid storage tank first liquid storage tank, the setting of first catheter and first pump is with the sample liquid inflow in the first liquid storage tank membrane changing filter, so that the millipore filtration membrane in the membrane changing filter catches bladder cancer cell. When the bladder cancer cell capturing device provided by the application captures bladder cancer cells, manual operation steps are reduced, the capturing difficulty of the bladder cancer cells is simplified, and the capturing efficiency of the bladder cancer cells is improved. The third reservoir may be configured to wash the fluid channel of the capture device with a buffer fluid in the third reservoir. Due to the arrangement of the membrane replacement filter, only the microporous filter membrane needs to be replaced when bladder cancer cells are captured, the material cost is low, and the waste of resources is reduced.
In one embodiment, the bladder cancer cell capturing device further comprises:
and the liquid inlet end of the fifth liquid guide pipe is connected with the liquid outlet of the membrane replacing filter, and the liquid at the liquid outlet of the membrane replacing filter flows to the waste liquid tank through the fifth liquid guide pipe.
In one embodiment, the bladder cancer cell capturing device further comprises:
and the oscillator is connected with the second liquid storage tank.
Specifically, can directly put into the second liquid storage tank with undiluted urine sediment sample liquid, through the leading-in second liquid storage tank of buffer solution in the fourth catheter with the third liquid storage tank to be used for diluting undiluted urine sediment sample liquid, the oscillator is connected with the second liquid storage tank, can make the liquid misce bene in the second liquid storage tank, thereby obtains the urine sediment sample liquid after diluting, so that the bladder cancer cell in the urine sediment sample liquid after the subsequent step dilutes. Improves the applicability of capturing bladder cancer cells from urinary sediment and improves the efficiency of capturing bladder cancer cells.
Example 2
According to example 1, one embodiment of this example provides a bladder cancer cell capture method comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch component closes the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
In the present embodiment, the bladder cancer cell capturing apparatus according to the present invention can capture bladder cancer cells in a urinary sediment diluent. After the microporous filter membrane is placed in the membrane replacing filter, the urinary sediment diluent flows into the first liquid storage tank from the second liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank so as to capture bladder cancer cells through the microporous filter membrane in the membrane replacing filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to example 1, one embodiment of this example provides a bladder cancer cell capture method comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a first valve, guiding the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
In the present embodiment, the bladder cancer cell capturing apparatus according to the present invention can capture bladder cancer cells in a urinary sediment diluent. After the microporous filter membrane is placed in the membrane replacing filter, the buffer solution is firstly led into the first liquid storage tank from the third liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank, so that bubbles on the microporous filter membrane in the membrane replacing filter are eliminated, and the capturing efficiency of bladder cancer cells is improved. And then the urinary sediment diluent flows into the first liquid storage tank from the second liquid storage tank and flows into the membrane changing filter from the first liquid storage tank so as to capture bladder cancer cells through a microporous filter membrane in the membrane changing filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to an embodiment of the bladder cancer cell capturing apparatus including the oscillator of example 1, one embodiment of this example provides a bladder cancer cell capturing method including:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch component closes the membrane replacing filter;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
In this embodiment, the capturing of bladder cancer cells in undiluted urine sediment can be achieved by the oscillator-containing bladder cancer cell capturing device of the present invention. The buffer solution in the third liquid storage tank is introduced into the second liquid storage tank, and the undiluted urinary sediment liquid in the second liquid storage tank is diluted and uniformly mixed through the work of the oscillator to obtain the corresponding urinary sediment diluent. After the microporous filter membrane is placed in the membrane replacement filter, diluting and uniformly mixing to obtain corresponding urinary sediment diluent, flowing into the first liquid storage tank from the second liquid storage tank, and flowing into the membrane replacement filter from the first liquid storage tank so as to capture bladder cancer cells through the microporous filter membrane in the membrane replacement filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
According to an embodiment of the bladder cancer cell capturing apparatus including the oscillator of example 1, one embodiment of this example provides a bladder cancer cell capturing method including:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch component closes the membrane replacing filter;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
opening a first valve, guiding the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
In this embodiment, the capturing of bladder cancer cells in undiluted urine sediment can be achieved by the oscillator-containing bladder cancer cell capturing device of the present invention. The buffer solution in the third liquid storage tank is introduced into the second liquid storage tank, and the undiluted urinary sediment liquid in the second liquid storage tank is diluted and uniformly mixed through the work of the oscillator, so that the corresponding urinary sediment diluent is obtained. After the microporous filter membrane is placed in the membrane replacing filter, the buffer solution is firstly led into the first liquid storage tank from the third liquid storage tank and flows into the membrane replacing filter from the first liquid storage tank, so that bubbles on the microporous filter membrane in the membrane replacing filter are eliminated, and the capturing efficiency of bladder cancer cells is improved. And then diluting and uniformly mixing to obtain corresponding urinary sediment diluent, flowing into the first liquid storage tank from the second liquid storage tank, and flowing into the membrane replacement filter from the first liquid storage tank so as to capture bladder cancer cells through a microporous filter membrane in the membrane replacement filter. In the process of capturing the bladder cancer cells, the manual operation steps are few, and the capturing efficiency of the bladder cancer cells is high.
Example 3
According to example 1, one embodiment of this example provides a method of cleaning a bladder cancer cell-trapping device, comprising:
the filter switch component turns on the membrane replacing filter; taking out the microporous filter membrane in the membrane replacing filter by a filter membrane taking-out device; the filter switch assembly turns off the membrane replacing filter;
putting the buffer solution into a third liquid storage tank;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump.
In this embodiment, with the bladder cancer cell capturing device provided herein, the second reservoir is washed by introducing the buffer solution in the third reservoir into the second reservoir; the buffer solution in the second liquid storage tank flows into the first liquid storage tank so as to clean the first liquid storage tank; and (4) enabling the buffer solution in the first liquid storage tank to flow into the membrane replacing filter so as to clean the interior of the membrane replacing filter. The cleaning device can clean the interiors of a liquid storage tank, a liquid guide pipe and a membrane replacing filter in the bladder cancer cell capturing device, so that the bladder cancer cell capturing device can be repeatedly used. Further improves the bladder cancer capturing efficiency of the bladder cancer cell capturing device provided by the application.
The features of the above embodiments may be arbitrarily combined, and for the sake of brevity, all possible combinations of the features in the above embodiments are not described, but should be construed as being within the scope of the present specification as long as there is no contradiction between the combinations of the features.
Although the embodiments of the present application have been described in connection with the accompanying drawings, it is not to be construed as limiting the scope of the claims. It should be noted that various changes and modifications to the above description could be made by those skilled in the art without departing from the spirit of the present application. And are neither required nor exhaustive of all embodiments. And obvious variations or modifications of this invention are intended to be covered by the scope of the invention as expressed herein. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A bladder cancer cell capture device, comprising:
a membrane replacement filter, wherein a microporous filter membrane is arranged in the membrane replacement filter and is used for capturing bladder cancer cells in liquid flowing through the membrane replacement filter;
the filter switch assembly is connected with the membrane replacing filter and is used for switching on or off the membrane replacing filter;
the filter membrane taking and placing device is used for taking and placing the microporous filter membrane;
the bottom of the first liquid storage tank is provided with a first liquid guide pipe, the first liquid guide pipe is connected with a liquid inlet of the membrane changing filter, liquid in the first liquid storage tank flows to the membrane changing filter through the first liquid guide pipe, and a first pump is arranged on the first liquid guide pipe; a second liquid guide pipe is arranged at the top of the first liquid storage tank, a second pump is arranged on the second liquid guide pipe, and liquid in a third liquid storage tank flows to the second liquid storage tank through the second liquid guide pipe;
the third liquid storage tank is arranged above the first liquid storage tank, a third liquid guide pipe and a fourth liquid guide pipe are arranged at the bottom of the third liquid storage tank, liquid in the third liquid storage tank flows to the first liquid storage tank through the third liquid guide pipe, liquid in the third liquid storage tank flows to the second liquid storage tank through the fourth liquid guide pipe, and a first valve is arranged on the third liquid guide pipe; a second valve is arranged on the fourth liquid guide pipe;
and the controller is connected with the filter membrane taking and placing assembly, the first pump, the second pump, the first valve and the second valve.
2. The bladder cancer cell capture device of claim 1, wherein the filter switch assembly comprises:
the positioning bracket is fixedly connected with the lower part of the membrane replacing filter, and the lower part of the membrane replacing filter is a part where a liquid outlet of the membrane replacing filter is positioned;
the shifting unit comprises a connecting support, a sliding block and a telescopic rod, the connecting support is connected with the positioning support, the sliding block is fixedly connected with the upper portion of the membrane replacing filter, the upper portion of the membrane replacing filter is the portion where the liquid inlet of the membrane replacing filter is located, a sliding groove is formed in the connecting support, the connecting support is connected with the sliding block in a sliding mode, the sliding block slides along the sliding groove, the sliding block is connected with the telescopic rod at the top of the connecting support, and the telescopic rod is connected with the controller.
3. The bladder cancer cell capture device of claim 1, wherein the filter pick-up device comprises a robotic arm configured to grasp a microporous filter membrane at a first location and place the grasped microporous filter membrane at a second location.
4. The bladder cancer cell capture device of claim 1, further comprising:
and the liquid inlet end of the fifth liquid guide pipe is connected with the liquid outlet of the membrane replacing filter, and the liquid at the liquid outlet of the membrane replacing filter flows to the waste liquid tank through the fifth liquid guide pipe.
5. The bladder cancer cell capture device of claim 1, further comprising:
an oscillator connected with the second reservoir.
6. The bladder cancer cell capturing apparatus according to any one of claims 1 to 5, a bladder cancer cell capturing method, comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
7. The bladder cancer cell capturing apparatus according to any one of claims 1 to 5, a bladder cancer cell capturing method, comprising:
putting a liquid sample to be captured into a second liquid storage tank, wherein the liquid sample to be captured is a urinary sediment diluent; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a first valve, guiding the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
8. The bladder cancer cell capturing apparatus according to claim 5, a bladder cancer cell capturing method, comprising:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch assembly turns off the membrane changing filter.
9. The bladder cancer cell capturing apparatus of any one of claims 1 to 5, a bladder cancer cell capturing method, comprising:
putting the urine sediment sample into a second liquid storage tank; putting the buffer solution into a third liquid storage tank;
the filter switch component turns on the membrane replacing filter; placing the microporous filter membrane into the membrane replacing filter by the filter membrane taking and placing device; the filter switch assembly turns off the membrane replacing filter;
opening a second valve, guiding the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting the oscillator, uniformly mixing the liquid in the second liquid storage tank, and closing the oscillator;
opening a first valve, leading the liquid in the third liquid storage tank into the first liquid storage tank, and closing the first valve;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacing filter;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
turning off the second pump, turning off the first pump;
the filter switch component turns on the membrane replacing filter; a filter membrane taking-out device takes out the microporous filter membrane which is captured with the bladder cancer cells in the membrane replacing filter; the filter switch component closes the membrane replacing filter.
10. The bladder cancer cell capture device of any one of claims 1 to 5, a method of cleaning a bladder cancer cell capture device, comprising:
the filter switch component turns on the membrane replacing filter; a filter membrane taking and placing device takes out the microporous filter membrane in the membrane replacing filter; the filter switch component closes the membrane replacing filter;
putting the buffer solution into a third liquid storage tank;
opening a second valve, leading the liquid in the third liquid storage tank into the second liquid storage tank, and closing the second valve;
starting a second pump to guide the liquid in the second liquid storage tank into the first liquid storage tank;
starting a first pump, and introducing liquid in the first liquid storage tank into the membrane replacement filter;
turning off the second pump, turning off the first pump.
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