CN215162760U - Real-time fluorescence quantitative PCR detection kit for thalassemia genes - Google Patents
Real-time fluorescence quantitative PCR detection kit for thalassemia genes Download PDFInfo
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- CN215162760U CN215162760U CN202121611345.2U CN202121611345U CN215162760U CN 215162760 U CN215162760 U CN 215162760U CN 202121611345 U CN202121611345 U CN 202121611345U CN 215162760 U CN215162760 U CN 215162760U
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- 108060003196 globin Proteins 0.000 claims abstract description 20
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Abstract
The utility model discloses a real-time fluorescence quantitative PCR detect reagent box of thalassemia gene, detect the dish including box body and PCR, the inside board of placing that is provided with of box body, place the inboard inside restriction hole that is provided with, restriction hole is provided with a plurality of, and every restriction downthehole portion all is provided with the reaction test tube, reaction test tube bottom is provided with the bottom plate, the bottom plate bottom is provided with movable lifter, activity lifter one side is provided with the fixture block, the meshing of fixture block one end has the gear, gear one end is rotated and is connected with driving motor, driving motor fixed connection is in the inside one side of box body, PCR detects the dish surface and is provided with the detection groove, it is provided with a plurality of, every to detect inslot portion all is provided with the culture solution, therefore whether contain gene chain fluorescent dye in the DNA fragment through detecting the reaction test tube and can detect out whether there is globin aplastic anemia (thalassemia) hereditary possibility .
Description
Technical Field
The utility model belongs to the technical field of medical science detects, concretely relates to thalassemia gene real-time fluorescence quantitative PCR detect reagent box.
Background
Thalassemia, the primary name of thalassemia, known as marine anemia, is a group of hereditary hemolytic anemia diseases, which is an anemia or pathological state caused by deficiency or insufficiency of synthesis of one or more globin chains in hemoglobin due to hereditary genetic defects, and due to the complexity and diversity of the genetic defects, the variability of the types, the quantities and the clinical symptoms of the deficient globin chains is large. The named and classified globin chains are lacked according to the types and the lacked degrees, and the clinical symptoms of the disease are not consistent, and the disease is mostly expressed as chronic progressive hemolytic anemia.
The molecular structure and synthesis of globin chain are determined by gene, if a couple is a gene carrier of homo-thalassemia, each time the couple is pregnant, the child has half chance to be normal, half chance to be the carrier, the other quarter chance to be a thalassemia major patient, the thalassemia is divided into four types of alpha type, beta type, delta beta type and delta type, wherein beta and alpha thalassemia are common, the molecular pathology of beta globin aplastic anemia (abbreviated as beta thalassemia) is quite complex, more than one hundred beta gene mutations are known, mainly due to point mutation of gene, and few gene deletions, most of alpha globin aplastic anemia (thalassemia) (abbreviated as alpha thalassemia) is due to deletion of alpha globin gene, and few are caused by gene point mutation. Due to the deletion of the white genes, a few of the genes are caused by gene point mutation, and in any case, huge economic loss and psychological burden are caused to families of patients, and in fact, most families cannot bear high medical expenses to cause the infant to die, and the prevention and reduction of the birth of the thalassemia infant are the fundamental method for solving the problem, so that the real-time fluorescence quantitative PCR detection kit for the thalassemia genes is provided.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, provide a real-time fluorescence quantitative PCR detect reagent box of thalassemia gene to solve that to what provide among the above-mentioned background art then very complicated to rare type and various types overlap the complex body that causes, clinical manifestation is different, only is according to clinical characteristics and routine laboratory hematology inspection the problem that can't diagnose.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a real-time fluorescence quantitative PCR detect reagent box of thalassemia gene, includes box body and PCR detection dish, the inside board of placing that is provided with of box body, place the inboard portion and be provided with the restriction hole, the restriction hole is provided with a plurality of, and every restriction downthehole portion all is provided with the reaction test tube, reaction test tube bottom is provided with the bottom plate, the bottom plate bottom is provided with movable lifter, activity lifter one side is provided with the fixture block, fixture block one end meshing has the gear, gear one end rotates and is connected with driving motor, driving motor fixed connection is in the inside one side of box body, PCR detection dish surface is provided with detects the groove, it is provided with a plurality of, every to detect the inslot portion all is provided with the culture solution.
Preferably, the placing plate is fixedly connected with the bottom plate through a connecting rod.
Preferably, the top end of the box body is hinged with a sealing cover, the top end of the sealing cover is fixedly connected with a lifting handle, and one side of the box body is provided with an observation panel.
Preferably, the inside temperature control device that is provided with of box body, temperature control device one end electric connection has adjust knob, adjust knob fixed connection is in the outside one side of box body.
Preferably, each reaction test tube bottom all is provided with the fixed slot, fixed connection is on the bottom plate upper surface in the fixed slot.
Preferably, the restriction enzyme is arranged inside the box body, and the polymerase is arranged inside the box body.
Preferably, the gene chain fluorescent dye is arranged in the box body, and the globin primer is arranged in the box body.
Compared with the prior art, the utility model provides a thalassemia gene real-time fluorescence quantitative PCR detect reagent box possesses following beneficial effect:
1. the utility model discloses a board of placing that sets up, will wait to detect inside the personnel's blood or red blood cell etc. injection reaction test tube, add the globin primer that sets up, because place board bottom fixedly connected with bottom plate, through the movable lifter that sets up of bottom plate, utilize the driving motor rotation of setting, can drive the gear rotation that driving motor one end rotation is connected, and then drive the fixture block of gear one end meshing and move, thereby drive the activity lifter shake from top to bottom, make the reaction test tube that sets up on placing the board rock evenly, can make the guide test sample take place the sudden change, through this way, the reaction to different site sudden change detections can be carried out in one or more reaction test tubes, can carry out multiunit experiments like this and contrast, increase the reliability of testing result;
2. the utility model discloses a reaction test tube that sets up, after the detection sample adds the globin primer and fully rocks, add restriction endonuclease in reaction test tube inside, utilize the inside reaction solution that sets up of restriction endonuclease and reaction test tube to cut the specific DNA sequence in blood or the red blood cell, then add the gene chain fluorochrome that sets up, make the specific DNA sequence under the cutting absorb gene chain fluorochrome at the split process, because do not have this section DNA sequence in the normal people's DNA, consequently whether contain gene chain fluorochrome in the DNA fragment through the detection reaction test tube can detect whether have the hereditary possibility of globin formation obstacle anemia (thalassemia);
3. the utility model discloses a PCR that sets up detects the dish, the blood solution in with the reaction test tube instils into PCR and detects the dish, add polymerase again, can sew up the DNA sequence after the cutting, the culture solution that the inslot portion that detects sets up is deuterogamied, can transcribe the globin DNA gene fragment that has gene chain fluorescent dye, can judge whether have and hereditary the possibility of mediterranean anemia through detecting the globin DNA gene fragment that transcribes, and simultaneously, utilize the temperature control device who sets up, can control the inside temperature of box body, thereby detect the globin transcription condition under different temperatures, through this kind of mode, as the control group, can reduce the influence of temperature to the testing result, the accuracy of testing result has further been improved.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description, do not constitute a limitation of the invention, in which:
FIG. 1 is a schematic structural diagram of a kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to the present invention;
FIG. 2 is a sectional view of the kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to the present invention;
FIG. 3 is an enlarged view of A in FIG. 2 of a kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to the present invention;
FIG. 4 is a top view of the PCR detection tray of the kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to the present invention;
in the figure: 1. a box body; 2. a PCR detection disc; 201. a detection tank; 202. a culture solution; 3. a sealing cover; 4. a viewing panel; 5. adjusting a knob; 6. a restriction enzyme; 7. a polymerase; 8. a gene chain fluorescent dye; 9. a globin primer; 10. placing the plate; 11. a connecting rod; 12. a base plate; 13. a limiting aperture; 14. a reaction tube; 15. lifting the handle; 16. fixing grooves; 17. a temperature control device; 18. a drive motor; 19. a gear; 20. a movable lifting rod; 21. and (7) clamping blocks.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a real-time fluorescence quantitative PCR detect reagent box of thalassemia gene, detect dish 2 including box body 1 and PCR, 1 inside being provided with of box body places board 10, place the inside restriction hole 13 that is provided with of board 10, restriction hole 13 is provided with a plurality of, every restriction hole 13 is inside all to be provided with reaction test tube 14, reaction test tube 14 bottom is provided with bottom plate 12, bottom plate 12 bottom is provided with movable lifter 20, movable lifter 20 one side is provided with fixture block 21, the meshing of fixture block 21 one end has gear 19, 19 one end of gear rotates and is connected with driving motor 18, 18 fixed connection of driving motor is in 1 inside one side of box body, 2 surfaces of PCR detect dish are provided with and detect groove 201, detect groove 201 and be provided with a plurality of, every detects the inside culture solution 202 that all is provided with of groove 201.
The utility model discloses in, preferred, place between board 10 and the bottom plate 12 through connecting rod 11 for fixed connection, 1 top of box body articulates there is sealed lid 3, 3 top fixedly connected with of sealed lid carry handle 15, 1 one side of box body is provided with observation panel 4, 1 inside temperature control device 17 that is provided with of box body, 17 one end electric connection of temperature control device has adjust knob 5, 5 fixed connection of adjust knob 5 are in 1 outside one side of box body.
The utility model discloses in, preferred, 14 bottoms of every reaction test tube all are provided with fixed slot 16, and fixed slot 16 fixed connection is at bottom plate 12 upper surface, and 1 inside restriction endonuclease 6 that is provided with of box body, 1 inside polymerase 7 that is provided with of box body, 1 inside gene chain fluorochrome 8 that is provided with of box body, 1 inside globin primer 9 that is provided with of box body.
The utility model discloses a theory of operation and use flow: when the kit is used, blood or red blood cells and the like of a person to be detected are injected into a reaction test tube 14, the arranged globin primer 9 is added, the reaction test tube 14 is placed on a placing plate 10, the arranged driving motor 18 is utilized to rotate, a gear 19 which is rotatably connected with one end of the driving motor 18 can be driven to rotate, a fixture block 21 which is meshed with one end of the gear 19 is driven to move, a movable lifting rod 20 is driven to vibrate up and down, the reaction test tube 14 which is arranged on the placing plate 10 is uniformly shaken, a restriction enzyme 6 is added into the reaction test tube 14, a specific DNA sequence in the blood or the red blood cells is cut by the restriction enzyme 6 and reaction liquid which is arranged in the reaction test tube 14, then the arranged gene chain fluorescent dye 8 is added, the cut specific DNA sequence absorbs the gene chain fluorescent dye 8 in the splitting process, and then the blood solution in the reaction test tube 14 is dripped into a PCR detection disc 2, polymerase 7 is added, the cut DNA sequence can be sutured, then the globin DNA gene fragment with the gene chain fluorescent dye 8 can be transcribed by matching with the culture solution 202 arranged in the detection groove 201, whether the globin DNA gene fragment has the possibility of genetic thalassemia or not can be judged by detecting the transcribed globin DNA gene fragment, meanwhile, the temperature in the box body 1 can be controlled by the arranged temperature control device 17, so that the globin transcription condition under different temperatures can be detected, and the method can be used as a control group to reduce the influence of the temperature on the detection result.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides a thalassemia gene real-time fluorescence quantitative PCR detect reagent box, includes box body (1) and PCR detection dish (2), its characterized in that: a placing plate (10) is arranged in the box body (1), a limiting hole (13) is arranged in the placing plate (10), a plurality of limiting holes (13) are arranged, a reaction test tube (14) is arranged in each limiting hole (13), the bottom end of the reaction test tube (14) is provided with a bottom plate (12), the bottom end of the bottom plate (12) is provided with a movable lifting rod (20), a clamping block (21) is arranged on one side of the movable lifting rod (20), one end of the clamping block (21) is engaged with a gear (19), one end of the gear (19) is rotatably connected with a driving motor (18), the driving motor (18) is fixedly connected with one side in the box body (1), the surface of the PCR detection disc (2) is provided with a detection groove (201), the detection groove (201) is provided with a plurality of grooves, and each detection groove (201) is internally provided with a culture solution (202).
2. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 1, which is characterized in that: the placing plate (10) is fixedly connected with the bottom plate (12) through a connecting rod (11).
3. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 2, wherein: the top end of the box body (1) is hinged with a sealing cover (3), the top end of the sealing cover (3) is fixedly connected with a lifting handle (15), and an observation panel (4) is arranged on one side of the box body (1).
4. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 3, wherein: the temperature control device (17) is arranged inside the box body (1), one end of the temperature control device (17) is electrically connected with the adjusting knob (5), and the adjusting knob (5) is fixedly connected to one side of the outside of the box body (1).
5. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 1, which is characterized in that: each reaction test tube (14) bottom all is provided with fixed slot (16), fixed slot (16) fixed connection is on bottom plate (12) upper surface.
6. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 5, wherein: the kit is characterized in that a restriction enzyme (6) is arranged in the kit body (1), and a polymerase (7) is arranged in the kit body (1).
7. The kit for real-time fluorescence quantitative PCR detection of thalassemia genes according to claim 6, wherein: the kit is characterized in that gene chain fluorescent dye (8) is arranged in the kit body (1), and globin primer (9) is arranged in the kit body (1).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121611345.2U CN215162760U (en) | 2021-07-15 | 2021-07-15 | Real-time fluorescence quantitative PCR detection kit for thalassemia genes |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121611345.2U CN215162760U (en) | 2021-07-15 | 2021-07-15 | Real-time fluorescence quantitative PCR detection kit for thalassemia genes |
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| CN215162760U true CN215162760U (en) | 2021-12-14 |
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| CN202121611345.2U Expired - Fee Related CN215162760U (en) | 2021-07-15 | 2021-07-15 | Real-time fluorescence quantitative PCR detection kit for thalassemia genes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115322883A (en) * | 2022-08-09 | 2022-11-11 | 苏州海苗生物科技有限公司 | Totally-enclosed multi-target nucleic acid isothermal amplification detection device |
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2021
- 2021-07-15 CN CN202121611345.2U patent/CN215162760U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115322883A (en) * | 2022-08-09 | 2022-11-11 | 苏州海苗生物科技有限公司 | Totally-enclosed multi-target nucleic acid isothermal amplification detection device |
| CN115322883B (en) * | 2022-08-09 | 2024-03-01 | 苏州海苗生物科技有限公司 | Totally-enclosed multi-target nucleic acid isothermal amplification detection device |
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| GR01 | Patent grant | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211214 |