CN214374317U - Detection card for rapidly detecting DNA methylation - Google Patents
Detection card for rapidly detecting DNA methylation Download PDFInfo
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- CN214374317U CN214374317U CN202120185254.0U CN202120185254U CN214374317U CN 214374317 U CN214374317 U CN 214374317U CN 202120185254 U CN202120185254 U CN 202120185254U CN 214374317 U CN214374317 U CN 214374317U
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Abstract
The utility model relates to the technical field of medical equipment, in particular to a detection card for rapidly detecting DNA methylation, which comprises an upper cover body and a lower cover body, wherein the upper surface of the upper cover body is provided with at least 3 colorimetric holes, detection holes and limiting holes, each detection hole corresponds to the position of the colorimetric hole one by one, and the straight line of each detection hole is parallel to the straight line of each colorimetric hole; the upper surface of the lower cover body is provided with colorimetric paper positioning grooves corresponding to the positions of the colorimetric holes, colorimetric paper is connected in the colorimetric paper positioning grooves in a sliding mode, pull pieces are arranged on two sides of the colorimetric paper, and the upper surface of the lower cover body is also provided with a plurality of detection shallow grooves and limiting deep grooves which are in one-to-one correspondence with the detection holes and the limiting holes respectively; the utility model has higher detection accuracy, can play a certain protection role on the color paper, prolongs the service life of the detection card, and can store the detection reagent by the placed liquid storage tube to prevent confusion; simple operation, long service life and low cost, and has important significance for early screening of tumors.
Description
Technical Field
The utility model relates to the technical field of medical equipment, in particular to a detection card for rapidly detecting DNA methylation.
Background
DNA methylation, one of the earliest discovered and most deeply studied epigenetic regulatory mechanisms, is a form of chemical modification of DNA, usually occurring at an early stage of tumor development, and thus detection of DNA methylation is of great importance for early screening of tumors.
Methods for detecting DNA methylation include fluorescence quantitative PCR, methylation sequencing and the like, but the methods are complex and long in time consumption, detection is carried out by means of a large instrument, and the cost is generally high; at present, there is also a method for rapidly detecting DNA methylation, which comprises the following detection principles: the nano gold particles can be aggregated under the induction of a salt solution, the nano gold adsorbing DNA can be protected by the DNA and can not be aggregated, the adsorption capacities of different methylation degrees of the DNA to the nano gold particles are different, and the adsorption capacity of the DNA methylation of tumor cells to the nano gold particles is strongest, so that the color of the nano gold solution with different aggregation degrees can be changed, the DNA methylation can be judged according to the change of the color of the solution, and the tumor can be predicted.
The patent (CN209636237U) discloses a detection card for rapidly detecting DNA methylation, but the structure setting is too simple, and the colorimetric paper is directly exposed on the side of the base, and is easily contaminated to cause color change, which affects the accuracy of the final detection result; and the nano gold solution is directly added into each tubular projection, so that the detection card can be used only once, and waste is caused.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to overcome the shortcoming of prior art, provide an easy operation, long service life, low cost, can be used to the detection card of the DNA methylation of the early examination of tumour, for realizing above-mentioned purpose, the utility model provides a detection card for short-term test DNA methylation, technical scheme is as follows:
a test card for rapid detection of DNA methylation comprising
The color comparison device comprises an upper cover body, wherein at least 3 color comparison holes are linearly and uniformly formed in one side of the upper surface of the upper cover body, a plurality of detection holes are formed in the upper surface of the upper cover body, the straight line where each detection hole is located is parallel to the straight line where each color comparison hole is located, the detection holes correspond to the color comparison holes in a one-to-one mode in position, color comparison can be well conducted through the corresponding arrangement, the detection accuracy is improved, and at least 3 limiting holes are formed in the other side of the upper surface of the upper cover body; and
lower lid, the upper surface of lid is equipped with colorimetric paper constant head tank down, just colorimetric paper constant head tank is corresponding with the position in colorimetric hole, sliding connection colorimetric paper in colorimetric paper constant head tank, just the both sides of colorimetric paper are equipped with and draw the piece to the drawing is compared the colorimetric paper and is slided at the colorimetric paper constant head tank, thereby carries out the judgement of colour, the upper surface of lid still is equipped with a plurality of detection shallow slot and spacing deep trouth down, detect the position one-to-one of shallow trouth position and inspection hole, the position one-to-one of position and spacing hole of spacing deep trouth.
Preferably, the lower surface of the upper cover body is provided with a plurality of fixing grooves, the upper surface of the lower cover body is provided with a plurality of fixing columns, and the fixing columns are matched with the fixing grooves, so that the upper cover body and the lower cover body are fixed.
Preferably, the upper cover body and the lower cover body are both rectangular, the number of the fixing grooves and the number of the fixing columns are 4, the fixing grooves are respectively arranged at four corners of the lower surface of the upper cover body, and correspondingly, the fixing columns are arranged at four corners of the upper surface of the lower cover body.
Preferably, the colorimetric holes, the detection holes and the detection shallow grooves are in one of a rectangular shape, a circular shape and an oval shape, and the limit holes and the limit deep grooves are in one of a rectangular shape and a circular shape.
Preferably, the both sides of lid are equipped with the side door respectively down, the notch phase-match of side door and color comparison paper constant head tank side, just the side door articulates on the notch of color comparison paper constant head tank, and the contrast color paper protects when not detecting, prevents the landing of color comparison paper, and prevents that impurity from getting into in the color comparison paper constant head tank.
Preferably, the pull member is a soft pull cord so that the presence of the pull member does not obstruct the opening/closing of the side door.
Preferably, the colorimetric paper is provided with at least 3 color display areas, two ends of the colorimetric paper are respectively a negative color display area and a positive color display area, and the middle part of the colorimetric paper is provided with at least one transition color display area.
Preferably, there are 3 said transition color display regions, and a plurality of said transition color display regions are present to facilitate the determination of the degree of DNA methylation.
Preferably, the number of the colorimetric holes is 4, multiple sets of detection can be performed simultaneously, correspondingly, the number of the detection holes is also 4, one of the detection holes is used as a control group detection hole, and the rest of the detection holes are used as sample detection holes, so that control can be set conveniently.
Preferably, the number of the limiting holes is 3, liquid storage pipes are arranged in the limiting holes and the limiting deep grooves corresponding to the limiting holes, and nanogold solution, water and salt solution are stored in the liquid storage pipes respectively.
Compared with the prior art, the beneficial effects of the utility model are that:
the utility model discloses a detection card for short-term test DNA methylation includes upper cover body and lower lid, through setting up color comparison paper constant head tank, sliding connection in the color comparison paper constant head tank color comparison paper and color comparison hole, sets up the color comparison paper in the color comparison paper constant head tank of lower lid, sets up the upper cover body above it to reach the certain guard action of contrast color paper, improved the life of detecting the card; the detection holes correspond to the colorimetric holes one by one, different colors of the colorimetric paper are displayed in the colorimetric holes by pulling the pulling pieces on the two sides of the colorimetric paper, so that the color consistent with the solution in the detection holes after reaction can be conveniently found, the observation effect is better, the detection accuracy is higher, and the DNA methylation degree can be better judged; the detection device is also provided with a limiting hole and a limiting deep groove corresponding to the limiting hole, and is used for placing a liquid storage pipe, and the nano-gold solution, water and a salt solution are respectively stored in the liquid storage pipe, so that the detection reagent is stored for repeated detection; the utility model discloses the setting of detection card is convenient for the detection of DNA methylation, and easy operation, long service life, low cost have important meaning to the early examination of tumour.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the detection card for rapidly detecting DNA methylation according to the present invention;
FIG. 2 is a schematic view of the front view structure of the bottom of the upper cover body of the present invention;
fig. 3 is a schematic front view of the colorimetric paper of the present invention;
in the figure: 1-upper cover body, 2-lower cover body, 3-colorimetric hole, 4-detection hole, 5-limiting hole, 6-fixed groove, 7-colorimetric paper positioning groove, 8-colorimetric paper, 9-detection shallow groove, 10-limiting deep groove, 11-fixed column, 12-drawing piece, 13-side door, 14-negative color display area, 15-transition color display area and 16-positive color display area.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Specific example 1:
fig. 1 is the utility model discloses a general structure schematic diagram of a detection card for short-term test DNA methylation, and combine fig. 2 and fig. 3, can see from it, the utility model discloses a detection card for short-term test DNA methylation, including upper cover body 1, be equipped with 4 circular color comparison holes 3 on one side of this upper cover body 1 upper surface linearly uniformly, be equipped with 4 circular shape inspection holes 4 on upper cover body 1 upper surface, the straight line that each inspection hole 4 belongs to is on a parallel with the straight line that each color comparison hole 3 belongs to, and each inspection hole 4 and the position one-to-one of color comparison hole 3, corresponding setting can carry out the comparison of colour betterly to improve the degree of accuracy that detects, one of them inspection hole 4 is as the check group inspection hole, remaining inspection hole 4 is as the sample inspection hole, is equipped with 3 circular shape spacing holes 5 on the opposite side of upper cover body 1 upper surface;
the upper surface of the lower cover body 2 is provided with a colorimetric paper positioning groove 7, the colorimetric paper positioning groove 7 corresponds to the position of the colorimetric hole 3, colorimetric paper 8 is connected in the colorimetric paper positioning groove 7 in a sliding manner, 5 color development areas are arranged on the colorimetric paper 8, two ends of the colorimetric paper are respectively a negative color display area 14 and a positive color display area 16, the middle part of the colorimetric paper 8 is provided with 3 transitional color display areas 15, the existence of the transitional color display areas is convenient for judging the DNA methylation degree, and two sides of the colorimetric paper 8 are provided with pulling pieces 12 with soft pulling ropes for pulling the colorimetric paper to slide in the colorimetric paper positioning groove, and the soft pulling ropes are arranged so that the pulling pieces do not block the opening/closing of a side door; the upper surface of the lower cover body 2 is also provided with 4 circular detection shallow grooves 9 and 3 circular limit deep grooves 10, wherein the positions of the detection shallow grooves 9 correspond to the positions of the detection holes 4 one by one, the positions of the limit deep grooves 10 correspond to the positions of the limit holes 5 one by one, liquid storage pipes are fixedly arranged in the limit deep grooves 10 and the limit holes 5 corresponding to the limit deep grooves 10, and nanogold solution, water and saline solution are respectively stored in the liquid storage pipes so as to facilitate detection; be equipped with side door 13 respectively in the both sides of lid 2 down, the notch phase-match of this side door 13 and 7 sides of colorimetric paper constant head tank, and this side door 13 articulates on the notch of colorimetric paper constant head tank 7 to the protection of contrast look paper when not detecting prevents the landing of colorimetric paper, and prevents that impurity from getting into in the colorimetric paper constant head tank.
In addition, in this embodiment, the upper cover body 1 and the lower cover body 2 are both rectangular, the four corners of the lower surface of the upper cover body 1 are respectively provided with the fixing grooves 6, the four corners of the upper surface of the lower cover body 2 are respectively provided with the fixing columns 11, and the fixing columns 11 are matched with the fixing grooves 6, so as to fix the upper cover body and the lower cover body.
Specific example 2:
fig. 1 is the utility model discloses a general structure schematic diagram of a detection card for short-term test DNA methylation, and combine fig. 2 and fig. 3, can see from it, the utility model discloses a detection card for short-term test DNA methylation, including upper cover body 1, be equipped with 4 rectangles color comparison hole 3 on one side of this upper cover body 1 upper surface linearly evenly, be equipped with 4 rectangles inspection hole 4 on upper cover body 1 upper surface, the straight line that each inspection hole 4 belongs to is on a parallel with the straight line that each color comparison hole 3 belongs to, and each inspection hole 4 and the position one-to-one of color comparison hole 3, the contrast of colour can be carried out better in corresponding setting to improve the degree of accuracy that detects, one of them inspection hole 4 is as the check group inspection hole, remaining inspection hole 4 is as the sample inspection hole, is equipped with 3 rectangle spacing holes 5 on the opposite side of upper cover body 1 upper surface;
the upper surface of the lower cover body 2 is provided with a colorimetric paper positioning groove 7, the colorimetric paper positioning groove 7 corresponds to the position of the colorimetric hole 3, colorimetric paper 8 is connected in the colorimetric paper positioning groove 7 in a sliding manner, 5 color development areas are arranged on the colorimetric paper 8, two ends of the colorimetric paper are respectively a negative color display area 14 and a positive color display area 16, the middle part of the colorimetric paper 8 is provided with 3 transitional color display areas 15, the existence of the transitional color display areas is convenient for judging the DNA methylation degree, and two sides of the colorimetric paper 8 are provided with pulling pieces 12 with soft pulling ropes for pulling the colorimetric paper to slide in the colorimetric paper positioning groove, and the soft pulling ropes are arranged so that the pulling pieces do not block the opening/closing of a side door; the upper surface of the lower cover body 2 is also provided with 4 rectangular detection shallow grooves 9 and 3 rectangular limit deep grooves 10, wherein the positions of the detection shallow grooves 9 correspond to the positions of the detection holes 4 one by one, the positions of the limit deep grooves 10 correspond to the positions of the limit holes 5 one by one, liquid storage pipes are fixedly arranged in the limit deep grooves 10 and the limit holes 5 corresponding to the limit deep grooves 10, and nanogold solution, water and saline solution are respectively stored in the liquid storage pipes so as to facilitate detection; be equipped with side door 13 respectively in the both sides of lid 2 down, the notch phase-match of this side door 13 and 7 sides of colorimetric paper constant head tank, and this side door 13 articulates on the notch of colorimetric paper constant head tank 7 to the protection of contrast look paper when not detecting prevents the landing of colorimetric paper, and prevents that impurity from getting into in the colorimetric paper constant head tank.
In addition, in this embodiment, the upper cover body 1 and the lower cover body 2 are both rectangular, the four corners of the lower surface of the upper cover body 1 are respectively provided with the fixing grooves 6, the four corners of the upper surface of the lower cover body 2 are respectively provided with the fixing columns 11, and the fixing columns 11 are matched with the fixing grooves 6, so as to fix the upper cover body and the lower cover body.
Specific example 3:
fig. 1 is the utility model discloses a general structure schematic diagram of a detection card for short-term test DNA methylation, and combine fig. 2 and fig. 3, can see from it, the utility model discloses a detection card for short-term test DNA methylation, including upper cover body 1, be equipped with 4 oval-shaped colorimetric hole 3 on one side of this upper cover body 1 upper surface linearly uniformly, be equipped with 4 oval-shaped inspection hole 4 on upper cover body 1 upper surface, the straight line that each inspection hole 4 belongs to is on a parallel with the straight line that each colorimetric hole 3 belongs to, and each inspection hole 4 and colorimetric hole 3's position one-to-one, the contrast of colour can be carried out better in corresponding setting to improve the degree of accuracy that detects, one of them inspection hole 4 is as the check group inspection hole, remaining inspection hole 4 is as the sample inspection hole, is equipped with 3 circular spacing holes 5 on the opposite side of upper cover body 1 upper surface;
the upper surface of the lower cover body 2 is provided with a colorimetric paper positioning groove 7, the colorimetric paper positioning groove 7 corresponds to the position of the colorimetric hole 3, colorimetric paper 8 is connected in the colorimetric paper positioning groove 7 in a sliding manner, 5 color development areas are arranged on the colorimetric paper 8, two ends of the colorimetric paper are respectively a negative color display area 14 and a positive color display area 16, the middle part of the colorimetric paper 8 is provided with 3 transitional color display areas 15, the existence of the transitional color display areas is convenient for judging the DNA methylation degree, and two sides of the colorimetric paper 8 are provided with pulling pieces 12 with soft pulling ropes for pulling the colorimetric paper to slide in the colorimetric paper positioning groove, and the soft pulling ropes are arranged so that the pulling pieces do not block the opening/closing of a side door; the upper surface of the lower cover body 2 is also provided with 4 elliptical detection shallow grooves 9 and 3 circular limit deep grooves 10, wherein the positions of the detection shallow grooves 9 correspond to the positions of the detection holes 4 one by one, the positions of the limit deep grooves 10 correspond to the positions of the limit holes 5 one by one, liquid storage pipes are fixedly arranged in the limit deep grooves 10 and the limit holes 5 corresponding to the limit deep grooves 10, and nanogold solution, water and saline solution are respectively stored in the liquid storage pipes so as to facilitate detection; be equipped with side door 13 respectively in the both sides of lid 2 down, the notch phase-match of this side door 13 and 7 sides of colorimetric paper constant head tank, and this side door 13 articulates on the notch of colorimetric paper constant head tank 7 to the protection of contrast look paper when not detecting prevents the landing of colorimetric paper, and prevents that impurity from getting into in the colorimetric paper constant head tank.
In addition, in this embodiment, the upper cover body 1 and the lower cover body 2 are both rectangular, the four corners of the lower surface of the upper cover body 1 are respectively provided with the fixing grooves 6, the four corners of the upper surface of the lower cover body 2 are respectively provided with the fixing columns 11, and the fixing columns 11 are matched with the fixing grooves 6, so as to fix the upper cover body and the lower cover body.
In the detection process, firstly adding a nano-gold solution into detection shallow grooves through each detection hole, then taking one detection hole as a control group detection hole, adding a certain amount of water into the detection shallow groove corresponding to the control group detection hole, adding a salt solution for reaction, taking the rest detection holes as sample detection holes, adding the same amount of DNA sample into the detection shallow groove corresponding to each sample detection hole for adsorption, adding the same amount of salt solution for reaction, at the moment, the color of the nano-gold solution can be seen to change in each detection shallow groove, next, judging the degree of DNA methylation according to the comparison of the color of the solution and colorimetric paper, and finally, finishing the detection of DNA methylation.
To sum up, as can be seen from the above embodiments of the present invention, the detection card for rapidly detecting DNA methylation of the present invention comprises an upper cover body and a lower cover body, wherein the colorimetric paper is disposed in the colorimetric paper positioning groove of the lower cover body by disposing the colorimetric paper positioning groove, the colorimetric paper slidably connected to the colorimetric paper positioning groove, and the colorimetric hole, and the upper cover body is disposed above the colorimetric paper positioning groove, thereby achieving a certain protection effect of the colorimetric paper and improving the service life of the detection card; the detection holes correspond to the colorimetric holes one by one, different colors of the colorimetric paper are displayed in the colorimetric holes by pulling the pulling pieces on the two sides of the colorimetric paper, so that the color consistent with the solution in the detection holes after reaction can be conveniently found, the observation effect is better, the detection accuracy is higher, and the DNA methylation degree can be better judged; the detection device is also provided with a limiting hole and a limiting deep groove corresponding to the limiting hole, and is used for placing a liquid storage pipe, and the nano-gold solution, water and a salt solution are respectively stored in the liquid storage pipe, so that the detection reagent is stored for repeated detection; the utility model discloses the setting of detection card is convenient for the detection of DNA methylation, and easy operation, long service life, low cost have important meaning to the early examination of tumour.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A test card for rapid detection of DNA methylation, comprising: comprises that
The color comparison cover comprises an upper cover body (1), wherein at least 3 color comparison holes (3) are linearly and uniformly formed in one side of the upper surface of the upper cover body (1), a plurality of detection holes (4) are formed in the upper surface of the upper cover body (1), the straight line where each detection hole (4) is located is parallel to the straight line where each color comparison hole (3) is located, the positions of each detection hole (4) and each color comparison hole (3) are in one-to-one correspondence, and at least 3 limiting holes (5) are formed in the other side of the upper surface of the upper cover body (1); and
lower lid (2), the upper surface of lower lid (2) is equipped with colorimetric paper constant head tank (7), just colorimetric paper constant head tank (7) is corresponding with the position than colored hole (3), colorimetric paper constant head tank (7) internal sliding connection compares colored paper (8), just the both sides than colored paper (8) are equipped with and draw piece (12), the upper surface of lower lid (2) still is equipped with a plurality of and detects shallow slot (9) and spacing deep groove (10), the position one-to-one of position and inspection hole (4) of detecting shallow slot (9), the position one-to-one of spacing deep groove (10) and the position one-to-one of spacing hole (5).
2. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the lower surface of the upper cover body (1) is provided with a plurality of fixing grooves (6), the upper surface of the lower cover body (2) is provided with a plurality of fixing columns (11), and the fixing columns (11) are matched with the fixing grooves (6).
3. The detection card for rapid detection of DNA methylation according to claim 2, characterized in that: the upper cover body (1) and the lower cover body (2) are both rectangular, the number of the fixing grooves (6) and the number of the fixing columns (11) are 4, the fixing grooves (6) are respectively arranged at four corners of the lower surface of the upper cover body (1), and correspondingly the fixing columns (11) are arranged at four corners of the upper surface of the lower cover body (2).
4. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the color comparison hole (3), the detection hole (4) and the detection shallow groove (9) are in one of a rectangular shape, a circular shape or an oval shape, and the limit hole (5) and the limit deep groove (10) are in one of a rectangular shape or a circular shape.
5. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the both sides of lower lid (2) are equipped with side door (13) respectively, side door (13) and the notch phase-match of color comparison paper constant head tank (7) side, just side door (13) articulate on the notch of color comparison paper constant head tank (7).
6. The detection card for rapid detection of DNA methylation according to claim 5, wherein: the pulling piece (12) is a soft pulling rope.
7. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the color comparison paper (8) is provided with at least 3 color development areas, the two ends of the color comparison paper (8) are respectively provided with a negative color display area (14) and a positive color display area (16), and the middle part of the color comparison paper (8) is provided with at least one transition color display area (15).
8. The detection card for rapid detection of DNA methylation according to claim 7, wherein: the number of the transitional color display areas (15) is 3.
9. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the number of the colorimetric holes (3) is 4, correspondingly, the number of the detection holes (4) is also 4, one detection hole (4) is used as a control group detection hole, and the rest detection holes (4) are used as sample detection holes.
10. The detection card for rapid detection of DNA methylation according to claim 1, characterized in that: the number of spacing hole (5) is 3, place the liquid storage pipe in spacing hole (5) and the spacing deep trouth (10) corresponding with spacing hole (5), just store nanogold solution, water and salt solution in the liquid storage pipe respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120185254.0U CN214374317U (en) | 2021-01-24 | 2021-01-24 | Detection card for rapidly detecting DNA methylation |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120185254.0U CN214374317U (en) | 2021-01-24 | 2021-01-24 | Detection card for rapidly detecting DNA methylation |
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| CN214374317U true CN214374317U (en) | 2021-10-08 |
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| CN202120185254.0U Active CN214374317U (en) | 2021-01-24 | 2021-01-24 | Detection card for rapidly detecting DNA methylation |
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