CN209636237U - It is a kind of for quickly detecting the detection card of DNA methylation - Google Patents
It is a kind of for quickly detecting the detection card of DNA methylation Download PDFInfo
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- CN209636237U CN209636237U CN201920255425.5U CN201920255425U CN209636237U CN 209636237 U CN209636237 U CN 209636237U CN 201920255425 U CN201920255425 U CN 201920255425U CN 209636237 U CN209636237 U CN 209636237U
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- detection card
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- tubular lobes
- dna methylation
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Abstract
The utility model discloses a kind of for quickly detecting the detection card of DNA methylation, the detection card includes pedestal, colorimetric, the colorimetric is fixed on side of base, and 2 or more tubular lobes, detection card described in the utility model are fixed on the pedestal, it can be convenient the detection for DNA methylation, it is easily stored, it is easy to operate, quickly, it is low in cost, it can be used for the early screening of tumour.
Description
Technical field
The utility model relates to medical instruments fields, and in particular to a kind of for quickly detecting the detection card of DNA methylation.
Background technique
DNA abnormal methylation usually occurs in the early stage of tumor development, thus the detection of DNA methylation is to tumour
Early screening is of great significance.The method that detection DNA methylation uses at present is usually quantitative fluorescent PCR and methylation sequencing
It needs Deng, these methods by large-scale instrument, it is complicated for operation, it takes a long time, testing cost is high.DNA different methylations
It is different with adsorption capacity of the methylation patterns to nanogold particle, absorption of the DNA methylation of tumour cell to nanogold particle
Ability is most strong.Nanogold particle salting liquid induction under can assemble, adsorbed DNA nanogold can by DNA protection and
Do not assemble.The color of the nano-Au solution of different aggregation extents can change, thus can be according to the change of solution colour
Change judges DNA methylation, to predict tumour.
However the detection card of DNA methylation detection it is not suitable at present, therefore develop a convenient for DNA methylation inspection
The detection card of survey reaches operation and is not required to by large-scale instrument, easy to operate, quickly, low in cost, can be used for the early stage sieve of tumour
The effect looked into, is of great significance.
Utility model content
DNA methylation is facilitated to detect to reach, the purpose of screening early-stage cancer, the utility model discloses one kind for fast
The detection card of speed detection DNA methylation reaches convenient and efficient detection DNA methylation level, the technical effect of screening cancer.
To achieve the above object, it is achieved by following technical solution:
It is a kind of for quickly detecting the detection card of DNA methylation, which is characterized in that detection card includes pedestal, colorimetric
Paper, the colorimetric are fixed on side of base, and 2 or more tubular lobes are fixed on the pedestal.
Preferably, the side of base is equipped with handle, and hand is facilitated to take.
Preferably, the handle is arc-shaped, and material is plastics.
Preferably, the pedestal is square plate, and material is opaque plastics, and plastics color is white, is easy to color
Observation.
Preferably, the tubular lobes are transparent plastic tubule, are easy to the observation of color.
Preferably, the tubular lobes are furnished with lid, have screw thread in the tubular lobes, mutually fit with the screw thread of cover inside
Match, after tubular lobes match upper cover, the reagent that inside is equipped with will not be poured, convenient for the packaging of product.
Preferably, the tubular lobes have 4, the respectively hole C, the hole T, water hole, salting liquid hole, wherein the hole C and T Kong Zhongjun
Equipped with nano-Au solution, the hole C is for doing check experiment, and the hole T is for detecting sample.
Preferably, the tubular lobes have 2, the respectively hole C, the hole T, and it is molten to be wherein respectively arranged with nanogold in the hole C and the hole T
Liquid.
Preferably, several reference colours are printed in the colorimetric, several reference colours include DNA methylation testing result
For negative color, positive color and several colors between negative and positive color.
The application method that card is detected described in utility model comprises the following steps:
1, extract DNA: samples sources can be tissue or cell, blood, urine, excrement etc..
2, detection card is taken out, lid is opened, the hole Kong Jiashui, T C is loaded this DNA extracted, mixes.
3, it is incubated for 5-10 minutes；
4, salting liquid is separately added into the hole C and the hole T；
5, the variation of solution colour in the hole C and the hole T is observed, and is compared with the color in colorimetric, determines result.
The utility model has the advantages that
The utility model provides a kind of detection card of quickly detection DNA methylation, and detection card operation is not required to by large size
Instrument, it is easily stored, it is easy to operate, it is quickly, low in cost, it can be used for the early screening of tumour.
Detailed description of the invention
Fig. 1 is that DNA methylation described in embodiment 1 detects card schematic top plan view；
Fig. 2 is that DNA methylation described in embodiment 1 detects card schematic side view；
Fig. 3 is that DNA methylation described in embodiment 2 detects card schematic top plan view；
In figure: 1. handles, 2. pedestals, 3. tubular lobes, 4. lids, 5. colorimetrics.
Specific embodiment
Embodiment 1
As shown in Figure 1 and Figure 2, a kind of for quickly detecting the detection card of DNA methylation, the detection card is by following 5 portions
Be grouped as: 1. handles, 2. pedestals, 3. tubular lobes, 4. lids, 5. colorimetrics, the colorimetric 5 are fixed on 2 side of pedestal, institute
It states and fixes 2 tubular lobes 3 on pedestal 2, in the groove on 3 bottom of the tubular lobes insertion pedestal 2,2 side of pedestal
Handle 1 is installed, the handle be it is arc-shaped, facilitate hand to take, material is plastics；The pedestal 2 is square plate, and material is not
Transparent plastic；The tubular lobes 3 are transparent plastic tubule, are fixed on pedestal 2, have screw thread in tubular lobes 3, with lid 4
Internal screw thread is adapted, and 2 tubular protrusions 3 are respectively the hole C and the hole T, and inside is respectively arranged with nano-Au solution, the hole C
It is used to detect sample for detection hole for doing control experiment, the hole T for control wells；The colorimetric 5 is pasted onto the one of pedestal
End, is above printed on several reference colours, and reference colour includes that DNA methylation test experience result is the several of negative and strong positive and centre
Kind color.
Nano-Au solution in the detection card hole T adsorbs the DNA being added in tubular lobes 3, then molten with the salt of addition
Liquid reaction, nano-Au solution color change, are compared according to solution colour with reference colour on colorimetric card, judge DNA first
Base degree.
Water is added in the nano-Au solution surveyed in the card hole C, then reacts with the salting liquid of addition, nano-Au solution color hair
Changing is compared with reference colour on colorimetric card according to solution colour, is judged as negative color.
The water and salting liquid use 2 reagent bottle encapsulation respectively.
Embodiment 2
As shown in figure 3, a kind of for quickly detecting the detection card of DNA methylation, detection card is made of following 5 parts:
1. handle, 2. pedestals, 3. 4 tubular lobes, 4. 4 lids, 5. colorimetrics.Four tubular lobes are respectively the hole C, T
Hole, water hole, salting liquid hole, the hole C are that control wells are used to detect sample for detection hole for doing control experiment, the hole T, institute
It states and is respectively arranged with nano-Au solution inside the hole C and the hole T；The handle be it is arc-shaped, facilitate hand to take, material is plastics, the handle 1
It is connected with pedestal 2；The pedestal is square plate, and material is opaque plastics；The tubular lobes are transparent plastic tubule, Gu
Due on pedestal, above there is screw thread, is adapted with the screw thread inside lid 4, it is recessed on 3 bottom of the tubular lobes insertion pedestal 2
In slot；The colorimetric 5 is pasted onto one end of pedestal, is above printed on several reference colours, and reference colour includes DNA methylation test experience
It as a result is negative and strong positive and several colors of centre；The water hole is equipped with water, and salting liquid hole is equipped with salting liquid.
Nano-Au solution in the detection card hole T adsorbs the DNA being added in tubular lobes 3, then molten with the salt of addition
Liquid reaction, nano-Au solution color change, are compared according to solution colour with reference colour on colorimetric card, judge DNA first
Base degree.
Water is added in nano-Au solution in the detection card hole C, then reacts with the salting liquid of addition, nano-Au solution color
It changes, is compared according to solution colour with reference colour on colorimetric card, is judged as negative color.
Water hole described in the present embodiment and salting liquid hole are respectively used to dress water and salting liquid, without in addition using reagent bottle
Dress, to avoid being scattered for reagent.The hole C and the hole T described in utility model may be it is multiple, when the hole T be it is multiple when, can be simultaneously
Detect multiple samples.
DNA described in embodiment 1 and embodiment 2, samples sources can be histocyte, blood, urine, excrement etc..
Obviously, those skilled in the art can carry out various modification and variations without departing from utility model to utility model
Spirit and scope.In this way, if these modification and variations of the utility model belong to the utility model claims and its wait
Within the scope of technology, then the utility model is intended to encompass these modification and variations.
Claims (10)
1. a kind of for quickly detecting the detection card of DNA methylation, which is characterized in that the detection card includes pedestal (2), colorimetric
Paper (5), the colorimetric (5) are fixed on pedestal (2) side, 2 or more tubular lobes (3) are fixed on the pedestal (2).
2. detection card according to claim 1, which is characterized in that pedestal (2) side is equipped with handle (1).
3. detection according to claim 2 card, which is characterized in that the handle (1) be it is arc-shaped, material is plastics.
4. detection card according to claim 1, which is characterized in that the pedestal (2) is square plate, and material is opaque
Plastics.
5. detection card according to claim 1, which is characterized in that the tubular lobes (3) are transparent plastic tubule.
6. detection card according to claim 1, which is characterized in that the tubular lobes (3) are furnished with lid (4), the pipe
There is screw thread in shape protrusion (3), the screw thread internal with lid (4) is adapted.
7. detection card according to claim 6, which is characterized in that the tubular lobes (3) have 4.
8. detection card according to claim 7, which is characterized in that 4 tubular lobes (3) are respectively the hole C, the hole T, water
Hole, salting liquid hole are wherein respectively arranged with nano-Au solution in the hole C and the hole T, and the hole C is for doing check experiment, and the hole T is for detecting
Sample.
9. detection card according to claim 6, which is characterized in that the tubular lobes (3) have 2, the respectively hole C, T
Hole is wherein respectively arranged with nano-Au solution in the hole C and the hole T, and the hole C is for doing check experiment, and the hole T is for detecting sample.
10. detection card according to claim 1, which is characterized in that be printed on several reference colours, institute on the colorimetric (5)
Stating several reference colours includes DNA methylation testing result for negative color, positive color and between negative and positive color
Between several colors.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920255425.5U CN209636237U (en) | 2019-02-28 | 2019-02-28 | It is a kind of for quickly detecting the detection card of DNA methylation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920255425.5U CN209636237U (en) | 2019-02-28 | 2019-02-28 | It is a kind of for quickly detecting the detection card of DNA methylation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209636237U true CN209636237U (en) | 2019-11-15 |
Family
ID=68494882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920255425.5U Active CN209636237U (en) | 2019-02-28 | 2019-02-28 | It is a kind of for quickly detecting the detection card of DNA methylation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209636237U (en) |
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2019
- 2019-02-28 CN CN201920255425.5U patent/CN209636237U/en active Active
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