CN206281697U - A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments - Google Patents
A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments Download PDFInfo
- Publication number
- CN206281697U CN206281697U CN201621284680.5U CN201621284680U CN206281697U CN 206281697 U CN206281697 U CN 206281697U CN 201621284680 U CN201621284680 U CN 201621284680U CN 206281697 U CN206281697 U CN 206281697U
- Authority
- CN
- China
- Prior art keywords
- extension
- orifice plates
- extension rod
- suitable pharmaceutical
- sample pre
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model is related to a kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments, including 96 orifice plates, extraction tube is provided with 96 orifice plate, extension is provided with the bottom of the extraction tube, the extension is connected arrangement with extraction tube, extension rod is provided with the extension, the length of the extension rod and the equal length of extension, arrangement is in close contact between the extension rod and extension, when extension rod under force, the extension rod can slide up and down arrangement in extension, backing plate is provided with below the extension and extension rod, after extension rod lower slider, extension rod is in contact and supported with backing plate.The utility model can realize that the sample cleanliness factor for extracting is high, be more suitable for detection and analysis and use.
Description
Technical field
The utility model is related to a kind of 96 orifice plate, more particularly to a kind of 96 holes of suitable Pharmaceutical Analysis sample pre-treatments
Plate.
Background technology
96 orifice plates are one piece of plastic boards, there is 96 apertures above, and each aperture can be as a small developmental tube.
The flat pattern and operating efficiency for being greatly improved experiment are applied in combination with Multi-channel liquid transfer device.96 orifice plates are of wide application
It is general, including concentration analysis, clinical detection, enzyme linked immunological etc..
The sample size of drug plasma analysis experiment is more, including complicated sample pre-treatments operation.In order to locate before improving sample
The efficiency of reason, 96 orifice plates are widely applied.
Blood plasma is a kind of extremely complex matrix of composition, contains substantial amounts of endogenous protein, polypeptide, hormone, various nutrition
Composition and cell metabolism waste.The detection and analysis of these materials often jamming target medicine.Therefore, drug plasma analysis experiment
The purpose of sample pre-treatments is, by sample cleanup, at utmost to remove endogenous matrix.Purer sample is more conducive to subsequently
Analysis detection.Specific practice is discarding in adding the organic reagent organic reagent that extracts medicine from blood plasma in blood plasma
Remaining endogenous matrix.
96 orifice plates are mainly suitable for two kinds of sample-pretreating methods, i.e. precipitation of protein and liquid-liquid extraction at present.
Precipitation of protein is a kind of method for adding albuminous degeneration reagent to remove removing protein in blood plasma.The purpose is to by blood plasma
Middle albumen is got rid of, and in extracting organic reagent with protein bound medicine, is finally reached the purpose of purifying or concentrating sample.
Idiographic flow is as follows：
Sample-adding：The blood plasma of certain volume is added in extraction vessel.
Add protein precipitant：Usually organic chemical reagent such as methyl alcohol, ethanol, acetonitrile etc., volume is more than blood plasma body
Long-pending 2 times.
Vortex：Vortex or mechanical shaking extraction container make blood plasma fully be mixed with extracts reagent.Protein in blood plasma is organic
It is solid particle that cohesion is denatured in the presence of reagent.
Centrifugation：Extraction vessel is centrifuged after being fully denatured for albumen, solid protein particle be in container bottom so as to extraction
Liquid is separated.
Separate：Follow-up analysis is carried out using pipettor extraction fluid to detect.Abandon remaining protein body.
Liquid-liquid extraction will be soluble in the medicine of organic reagent by adding water insoluble organic reagent in blood plasma
Extracted from blood plasma.Its key step is as follows：
Sample-adding：The blood plasma of certain volume is added in extraction vessel.
Add extracts reagent：Usually organic chemical reagent has ethyl acetate, ethers, n-hexane etc..
Vortex is extracted：Vortex or mechanical shaking extraction container make blood plasma be fully contacted with extracts reagent.
Centrifugation layering：After centrifugation, the liquid in container is divided into two-layer to extraction vessel, the extracts reagent of most species it is close
Degree is smaller than water in upper strata, and water layer is in lower floor.Medicine containing detection in need in the extracts reagent of upper strata.Contain in lower aqueous solution
There is the most of endogenous matrix in blood plasma, these endogenous matrix are interfered to analysis detection.
Separate：Separating the extract solution in upper strata using pipettor carries out follow-up concentration analysis detection.Lower floor is abandoned to contain
The aqueous solution of matrix.
The space of a whole page size of conventional 96 orifice plates is about 128 × 86mm, has concentrated 96 sample extraction holes, and density is very big.Big portion
Sample state of the office in interstitial hole is difficult to be observed from side.96 orifice plates of conventional plasma drug level analysis are poly- third
Alkene, material translucency is very poor, causes not only center section sample to observe its state from side, and the aperture at edge cannot also be seen
Examine.The diameter in other 96 hole is thinner, also cannot almost observe the sample of drawing holes bottom during extraction fluid from surface
State.
Obstruction in this sight line greatly reduces application of 96 orifice plates in sample pretreatment process.
When preprocess method is albumen precipitation, when protein precipitation terminates rear extraction fluid, it is difficult to observe bottom egg
The line of demarcation of white precipitation particle and extract solution.It is difficult to judge the height that pipette inserts liquid level during using pipettor extraction fluid
Degree.If the height of pipette insertion liquid level is about deep.The granular precipitates of its bottom float under the flow action that siphon is produced
Float, into pipette.These granular precipitates may block pipette causes imbibition volume error occur.Particulate matter
It is also possible to block analytical instrument during subsequent analysis, prevents instrument from normally using or damaging.
When preprocess method is liquid-liquid extraction, it is impossible to observe the aqueous solution of upper strata extracts reagent and lower floor containing matrix it
Between line of demarcation, mask work is become very difficult.If having drawn the aqueous solution of the lower floor containing endogenous matrix, it is easy to dry
Disturb the detection of sample.
After extraction fluid, follow-up concentration can be carried out, uprise the concentration in extract solution, be conducive to inspection
Survey.That is, separating, acquisition extracting liquid volume is bigger, concentration is higher after concentration.But there is obstruction in 96 hole sight lines, in order to avoid
The water layer of albumen precipitation particle or lower floor containing endogenous matrix is drawn onto during extraction fluid, testing crew can only separate acquisition one
Divide extract solution, abandon many extract solutions.In order to obtain more extract solutions, testing crew often selects transparent centrifuge tube to make
For extraction vessel substitutes 96 orifice plates.Due to each centrifuge tube can only extract a sample, it is necessary to it is substantial amounts of muscle power operation as coding,
Single tube is loaded and single tube liquid relief.The flux of whole sample pre-treatments is substantially reduced.
Utility model content
In order to solve the above technical problems, the purpose of this utility model is to provide a kind of 96 orifice plates of suitable protein precipitation.
To achieve the above object, the utility model is adopted the following technical scheme that：
A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments, including 96 orifice plates, are provided with 96 orifice plate and carry
Pipe is taken, extension is provided with the bottom of the extraction tube, the extension is connected arrangement with extraction tube, in the extension
Extension rod, the length of the extension rod and the equal length of extension are provided with, are closely connect between the extension rod and extension
Touch arrangement, when extension rod under force, the extension rod can slide up and down arrangement in extension, the extension with prolong
Backing plate is provided with below boom, after extension rod lower slider, extension rod is in contact and supported with backing plate.
Further, 96 orifice plates of described suitable protein precipitation, wherein, the material of the extension rod is glass, or is
The polypropylene of polished treatment.
Further, 96 orifice plates of described suitable Pharmaceutical Analysis sample pre-treatments, wherein, the material of the extension
Matter is polypropylene.
Further, 96 orifice plates of described suitable Pharmaceutical Analysis sample pre-treatments, wherein, the two of 96 orifice plate
Side is provided with support column, and backing plate is set on the support columns.
Again further, 96 orifice plates of described suitable Pharmaceutical Analysis sample pre-treatments, wherein, for installing backing plate
The side of the support column be marked with graduated scale.
Again further, 96 orifice plates of described suitable Pharmaceutical Analysis sample pre-treatments, wherein, the backing plate is provided with
If dried layer, if by dried layer stacked arrangement, and every layer of thickness is equal.
Again further, 96 orifice plates of described suitable Pharmaceutical Analysis sample pre-treatments, wherein, the backing plate grafting
On the support columns.
By such scheme, the utility model at least has advantages below：
The utility model increased the extension of small internal diameter below extraction tube, increased space for deposit precipitate egg
Lower floor's aqueous solution containing matrix of white particle or liquid-liquid extraction, is notified coordinating the use of extension rod, and above-mentioned substance is sealed up for safekeeping
In extension.Separate extracts reagent is needed to form separation spatially with top.When pre-treating method is protein precipitation, point
It is difficult to be drawn to protein body during from extract solution, more preferably, sample cleanliness factor is greatly improved pipetting volume uniformity, is not easily blocked point
Analyzer device causes loss.When pre-treating method is liquid-liquid extraction, the water layer containing endogenous matrix is concentrated in extension and organic
Up in extraction tube, contact area is greatly reduced and formed and spatially separates extract solution between the two.During separation and Extraction reagent
It is difficult to be drawn onto the matrix of lower floor.Sample cleanliness factor is high, is more suitable for detection and analysis.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of the utility model embodiment, below will be to be used needed for embodiment
Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiments of the present utility model, therefore should not be by
Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, may be used also
Other related accompanying drawings are obtained with according to these accompanying drawings.
Fig. 1 is structural representation of the present utility model；
Structural representation after extension rod is in contact with backing plate when Fig. 2 is work of the present utility model；
Fig. 3 is 96 orifice plate planing surface figures after the centrifugation of protein precipitation method；
Fig. 4 is 96 orifice plate planing surface figures after liquid-liquid extraction centrifugation.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is described in further detail.Below
Embodiment is used to illustrate the utility model, but is not limited to scope of the present utility model.
In order that those skilled in the art more fully understand the utility model scheme, below in conjunction with the utility model reality
Accompanying drawing in example is applied, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that described reality
Example only a part of embodiment of the utility model is applied, rather than whole embodiments.Described in accompanying drawing and show generally herein
The component of the utility model embodiment for going out can be arranged and designed with a variety of configurations.Therefore, below in accompanying drawing
The detailed description of the embodiment of the present utility model of middle offer is not intended to limit claimed scope of the present utility model, and
It is to be merely representative of selected embodiment of the present utility model.Based on embodiment of the present utility model, those skilled in the art are not having
The every other embodiment obtained on the premise of making creative work, belongs to the scope of the utility model protection.
Embodiment
As shown in Figure 1 and Figure 2, a kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments, including 96 orifice plates, described 96
Extraction tube 1 is provided with orifice plate, extension 2 is provided with the bottom of the extraction tube 1, the extension 2 is connected with extraction tube 1
Logical arrangement, is provided with extension rod 3, the length of the extension rod 3 and the equal length of extension 2 in the extension 2, described to prolong
Between boom 3 and extension 2 be in close contact arrangement, when extension rod 3 under force, the extension rod 3 can be in extension 2
Slide up and down arrangement, the lower section of the extension 2 and extension rod 3 is provided with backing plate 4, after 3 lower slider of extension rod, with backing plate 4
It is in contact and supports extension rod 3.Coupled extension 2 is provided with by the bottom of extraction tube 1, and in extension 2
Extension rod is equiped with, and it is in close contact therewith, while can also be slided in extension under force, such that it is able to
Allow extension 2 built with sediment, the sample cleanliness factor of extraction is high, be more suitable for detection and analysis and use.
The material of extension rod 3 described in the utility model is glass, or is the polypropylene of polished treatment, while described prolong
The material for stretching pipe 2 is polypropylene.
The both sides of 96 orifice plates described in the utility model are provided with support column 5, and backing plate 4 is arranged on support column 5, and described
Backing plate 4 is plugged on support column 5, the convenient installation and removal to backing plate 4.
The side of the support column 5 in the utility model for installing backing plate 4 is marked with graduated scale, is easy to operating personnel
The thickness of backing plate 4 can accurately be controlled, so that maximized improve the sample cleanliness factor for extracting.
If backing plate 4 described in the utility model is provided with dried layer, if by dried layer stacked arrangement, and every layer of thickness is equal,
Facilitating operating personnel can carry out the operation of different capabilities to different requirements.
Operation principle of the present utility model is as follows：
During work, as shown in Fig. 2 needing to carry out sample before processing in extraction tube 1, increase extracts reagent, by one
After the reaction of section time, sample is centrifuged, it is not necessary to a part be sunken to the bottom of extraction tube 1, and extract solution is in the above,
Because unwanted part is after the bottom of extraction tube 1 is sunken to, by the connection with extension 2, it is submerged into extension 2
It is interior, after sinking in extension 2, the slide downward of extension rod 3 in extension 2 will be promoted under the gravity of its own, until extending
The lower end of bar 3 is in contact with backing plate 4, and now a unwanted part is nearly all moved into extension 2, and then operator is just
Can be extracted.
Embodiment 1
As shown in figure 3, when being extracted to 100 microlitres of plasma samples using protein precipitation method, addition 100 is micro- in extraction tube 1
Rise plasma sample, add 400 microlitres of methyl alcohol, 96 orifice plates are placed in eddy oscillating 5min on rocker device.Egg in plasma sample
Particle is formed after being fully denatured through methyl alcohol in vain, while 50 microlitres of specification backing plates 4 are installed, 4500 revs/min of centrifugations in centrifuge
After 10min, extension rod 3 is displaced downwardly at 50 microlitres of scales.Protein body volume is about 20 microlitres and a small amount of extract solution, and (about 30 is micro-
Rise) enter in extension 2, protein body is produced with extract solution and spatially separated, and the top extracting liquid volume of extension 2 is 450
Microlitre, pipetting 400 microlitres of extract solutions in another common 96 orifice plate is carried, extract solution is limpid, without particulate matter and volume is consistent.
Embodiment 2
As shown in figure 4, when being extracted to 100 microlitres of plasma samples using liquid-liquid extraction, addition 100 is micro- in extraction tube 1
Rise blood plasma, be separately added into 100 microlitres of water and 600 microlitres of ethyl acetate again, 96 orifice plates are placed in eddy oscillating on rocker device
15min.200 microlitres of backing plates of specification are installed.4500 revs/min of centrifugation 10min in centrifuge.It is micro- that extension rod 3 is displaced downwardly to 200
Rise at scale, the aqueous solution containing endogenous matrix is concentrated in extension, and extracting liquid volume is about 600 microlitres in extraction tube 1, with
Upper strata extract solution produces separation spatially.When pipetting 500 microlitres of extract solutions, in pipette sample it is limpid, without lower floor's liquid.
It is adapted to subsequent analysis detection work.
The utility model increased the extension of small internal diameter below extraction tube, increased space for deposit precipitate egg
Lower floor's aqueous solution containing matrix of white particle or liquid-liquid extraction, is notified coordinating the use of extension rod, and above-mentioned substance is sealed up for safekeeping
In extension.Separate extracts reagent is needed to form separation spatially with top.When pre-treating method is protein precipitation, point
It is difficult to be drawn to protein body during from extract solution, more preferably, sample cleanliness factor is greatly improved pipetting volume uniformity, is not easily blocked point
Analyzer device causes loss.When pre-treating method is liquid-liquid extraction, the water layer containing endogenous matrix is concentrated in extension and organic
Up in extraction tube, contact area is greatly reduced and formed and spatially separates extract solution between the two.During separation and Extraction reagent
It is difficult to be drawn onto the matrix of lower floor.Sample cleanliness factor is high, is more suitable for detection and analysis.
The above is only preferred embodiment of the present utility model, is not limited to the utility model, it is noted that
For those skilled in the art, on the premise of the utility model know-why is not departed from, can also do
Go out some improvement and modification, these are improved and modification also should be regarded as protection domain of the present utility model.
Claims (7)
1. a kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments, including 96 orifice plates, it is characterised in that：On 96 orifice plate
Extraction tube (1) is provided with, extension (2), the extension (2) and extraction tube (1) are provided with the bottom of the extraction tube (1)
Be connected arrangement, and extension rod (3), the length of the extension rod (3) and the length of extension (2) are provided with the extension (2)
Degree it is equal, between the extension rod (3) and extension (2) be in close contact arrange, when extension rod (3) under force, it is described
Extension rod (3) can slide up and down arrangement in extension (2), and backing plate is provided with below the extension (2) and extension rod (3)
(4), after extension rod (3) lower slider, extension rod (3) is in contact and supported with backing plate (4).
2. 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments according to claim 1, it is characterised in that：The extension
The material of bar (3) is glass, or is the polypropylene of polished treatment.
3. 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments according to claim 1, it is characterised in that：The extension
The material for managing (2) is polypropylene.
4. 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments according to claim 1, it is characterised in that：96 hole
The both sides of plate are provided with support column (5), and backing plate (4) is arranged on support column (5).
5. 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments according to claim 4, it is characterised in that：For installing
The side of the support column (5) of backing plate (4) is marked with graduated scale.
6. 96 orifice plates of the suitable Pharmaceutical Analysis sample pre-treatments according to claim 1,4 or 5, it is characterised in that：Institute
Backing plate (4) is stated if being provided with dried layer, if by dried layer stacked arrangement, and every layer of thickness is equal.
7. 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments according to claim 6, it is characterised in that：The backing plate
(4) it is plugged on support column (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621284680.5U CN206281697U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621284680.5U CN206281697U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206281697U true CN206281697U (en) | 2017-06-27 |
Family
ID=59074310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621284680.5U Active CN206281697U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206281697U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106908513A (en) * | 2017-03-09 | 2017-06-30 | 浙江大学 | It is a kind of for 96 hole high flux solid-phase extraction devices of mass spectral analysis and its application |
-
2016
- 2016-11-28 CN CN201621284680.5U patent/CN206281697U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106908513A (en) * | 2017-03-09 | 2017-06-30 | 浙江大学 | It is a kind of for 96 hole high flux solid-phase extraction devices of mass spectral analysis and its application |
| CN106908513B (en) * | 2017-03-09 | 2019-08-06 | 杭州汇健科技有限公司 | It is a kind of for 96 hole high throughput solid-phase extraction devices of mass spectral analysis and its application |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9804185B2 (en) | Application method for automatic micro droplet array screening system with picoliter scale precision | |
| CN106053859B (en) | A kind of centrifugal glycosylated hemoglobin detection micro-fluidic chip | |
| CN101838609B (en) | Sample adding device and application thereof | |
| US10195547B2 (en) | Method and system for buoyant separation | |
| ATE365909T1 (en) | APPARATUS AND METHOD FOR MIXING SAMPLES IN VESSELS | |
| CN102759474A (en) | Liquid-based cell smear manufacturing and dyeing machine utilizing principles of injection and pipetting | |
| CN206281697U (en) | A kind of 96 orifice plates of suitable Pharmaceutical Analysis sample pre-treatments | |
| Makde et al. | Liquid-based cytology: Technical aspects | |
| US20130040403A1 (en) | Method and apparatus for automated analysis | |
| EP2840398B1 (en) | Sample preparation workstation | |
| JP4732913B2 (en) | Microchip and method of using microchip | |
| CN207516132U (en) | A kind of multi-functional Tip SPE of high throughput ultramicron | |
| CN1331608C (en) | Hollow membraned tubular micro-extraction analyzer and application method thereof | |
| CN101539583A (en) | Automatic analyzer | |
| CN210198828U (en) | Full-automatic QuEChERS pretreatment all-in-one machine based on magnetic separation | |
| CN108562743B (en) | Modular chamber and application thereof in efficient capture of rare cells in blood | |
| CN206960208U (en) | A kind of suck-back Combined water spline filter system | |
| CN1663662A (en) | Hanging drop type liquid-liquid micro-extraction method | |
| CN205127983U (en) | Container of multichannel application of sample ware is exclusively used in | |
| CN206223520U (en) | A kind of 96 orifice plates of suitable protein precipitation | |
| CN205127997U (en) | Dispersion liquid phase centrifuging tube for micro -extraction | |
| CN205049516U (en) | Adopt unicellular gel electrophoresis to detect kit of DNA chain interruption | |
| CN214654868U (en) | Circulating tumor cell detection kit | |
| CN111638114A (en) | Full-automatic QuEChERS pretreatment all-in-one machine based on magnetic separation and pretreatment method thereof | |
| CN211627586U (en) | Human B cell maturation antigen detection kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yang Yong Inventor after: Zhong Kan Inventor after: Chen Tao Inventor after: Hu Fengmei Inventor after: Su Caijuan Inventor after: Xiao Tao Inventor after: Niu Xiaoying Inventor before: Yang Yong |