CN206223520U - A kind of 96 orifice plates of suitable protein precipitation - Google Patents
A kind of 96 orifice plates of suitable protein precipitation Download PDFInfo
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- CN206223520U CN206223520U CN201621287361.XU CN201621287361U CN206223520U CN 206223520 U CN206223520 U CN 206223520U CN 201621287361 U CN201621287361 U CN 201621287361U CN 206223520 U CN206223520 U CN 206223520U
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- main pipe
- auxiliary
- elastic packing
- storehouse
- orifice plates
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Abstract
The utility model is related to a kind of 96 orifice plates of suitable protein precipitation, including main pipe, the bottom of the main pipe offers an opening, the auxiliary pipe storehouse being seamlessly connected with main pipe is provided with the opening, auxiliary Guan Cangyu main pipes are made to be connected arrangement, elastic packing door is provided with the opening that main pipe described in the auxiliary Guan Cangyu is connected, make auxiliary Guan Cangyu main pipes spaced apart, when centrifugal force suffered by the liquid and protein body in main pipe more than elastic packing door it is elastic after, inwall direction from elastic packing door to auxiliary pipe storehouse open arrange.The utility model pipette in pipetting processes is difficult to touch precipitation particle, and particle is in have with the liquid in main pipe in auxiliary pipe storehouse and spatially separates in addition, is hardly influenceed by siphon generation current, and particle is difficult floating.Imbibition volume more accurate, sample is cleaner, be adapted to analysis detection.
Description
Technical field
The utility model is related to a kind of orifice plate, more particularly to a kind of 96 orifice plates of suitable protein precipitation.
Background technology
Precipitation of protein is a kind of conventional sample-pretreating method of plasma sample drug concentration analysis.The purpose is to incite somebody to action
Albumen is got rid of in blood plasma, in extracting organic reagent with protein bound medicine, is finally reached purifying or concentrating sample
Purpose.
Idiographic flow is as follows：
Sample-adding：The blood plasma of certain volume is added in extraction vessel.
Add protein precipitant：Usually organic chemical reagent such as methyl alcohol, ethanol, acetonitrile etc., volume is more than blood plasma body
Long-pending 2 times.
Vortex：Vortex or mechanical shaking extraction container make blood plasma fully be mixed with extracts reagent.Protein in blood plasma is organic
It is solid particle that cohesion is denatured in the presence of reagent.
Centrifugation：Extraction vessel is centrifuged after being fully denatured for albumen, solid protein particle be in container bottom so as to extraction
Liquid is separated.
Separate：The extract solution for finally being separated above protein body using pipettor is carried out follow-up analysis and detected.
Mainly there are small size centrifuge tube, or 96 orifice plates currently used for the container of the extraction of albumen precipitation.
Each boreliquid capacity of 96 conventional orifice plates is 1 or 2mL.Cube or cylinder, bottom are shaped as in each hole
Portion is U-shaped.The material of 96 orifice plates is mainly polypropylene.When carrying out albumen precipitation treatment, every piece of 96 orifice plates can simultaneously carry out 96
The extraction of sample.
When protein precipitation terminates rear extraction fluid, due to the design feature of 96 orifice plates, it is difficult to observe intermediate portion
Hole in line of demarcation between bottom precipitation and extract solution.During using pipettor, the granular precipitates of bottom are produced in siphon
Flow action under float, into pipette.These granular precipitates may block pipette causes imbibition volume
There is error.Particulate matter is also possible to block analytical instrument during subsequent analysis, prevents instrument from normally using or damaging.
In view of above-mentioned defect, the design people is actively subject to research and innovation, to found a kind of being adapted to for new structure
96 orifice plates of protein precipitation, make it have more the value in industry.
Utility model content
In order to solve the above technical problems, the purpose of this utility model is to provide a kind of 96 orifice plates of suitable protein precipitation.
To achieve the above object, the utility model is adopted the following technical scheme that：
A kind of 96 orifice plates of suitable protein precipitation, including main pipe, the bottom of the main pipe offer an opening, described
The auxiliary pipe storehouse being seamlessly connected with main pipe is provided with opening, auxiliary Guan Cangyu main pipes is connected arrangement, in the auxiliary pipe storehouse
Elastic packing door is provided with the opening being connected with the main pipe, makes auxiliary Guan Cangyu main pipes spaced apart, work as supervisor
After internal albumen gravity is more than the elasticity of elastic packing door, elastic packing door opens cloth to the inwall direction in auxiliary pipe storehouse
Put.
Further, 96 orifice plates of described suitable protein precipitation, wherein, the auxiliary Guan Cangyu main pipes are formed in one
Arrangement.
Further, 96 orifice plates of described suitable protein precipitation, wherein, the opening of the elastic packing door is one
Font, or for cross.
Further, 96 orifice plates of described suitable protein precipitation, wherein, the material of the elastic packing door is soft
Silica gel, or be thin polymer film.
Again further, 96 orifice plates of described suitable protein precipitation, wherein, the elastic packing door is socketed in institute
On the opening of the bottom for stating main pipe, when main pipe is equipped with liquid, main pipe is separated close by elastic packing door with auxiliary pipe storehouse
The disconnected arrangement of packing.
Again further, 96 orifice plates of described suitable protein precipitation, wherein, the shape in the auxiliary pipe storehouse is in cylinder
Or cone arrangement.
By such scheme, the utility model at least has advantages below：
The utility model pipette in pipetting processes is difficult to touch precipitation particle, in addition particle be in auxiliary pipe storehouse in and
Liquid in main pipe has separation spatially, is hardly influenceed by siphon generation current, and particle is difficult floating.Imbibition body
Product is more accurate, parallel.Sample cleanliness factor greatly improves, hardly have particulate material is present in the extract solution after separating,
It is not easily blocked analytical instrument.
Described above is only the general introduction of technical solutions of the utility model, in order to better understand skill of the present utility model
Art means, and being practiced according to the content of specification, with preferred embodiment of the present utility model and coordinate accompanying drawing detailed below
Describe in detail bright as after.
Brief description of the drawings
In order to illustrate more clearly of the technical scheme of the utility model embodiment, below will be to be used needed for embodiment
Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiments of the present utility model, therefore should not be by
Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, may be used also
Other related accompanying drawings are obtained with according to these accompanying drawings.
Fig. 1 is structural representation of the present utility model；
Structural representation after elastic packing door is opened when Fig. 2 is work of the present utility model.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is described in further detail.Below
Embodiment is used to illustrate the utility model, but is not limited to scope of the present utility model.
In order that those skilled in the art more fully understand the utility model scheme, below in conjunction with the utility model reality
Accompanying drawing in example is applied, the technical scheme in the utility model embodiment is clearly and completely described, it is clear that described reality
Example only a part of embodiment of the utility model is applied, rather than whole embodiments.Described in accompanying drawing and show generally herein
The component of the utility model embodiment for going out can be arranged and designed with a variety of configurations.Therefore, below in accompanying drawing
The detailed description of the embodiment of the present utility model of middle offer is not intended to limit claimed scope of the present utility model, and
It is to be merely representative of selected embodiment of the present utility model.Based on embodiment of the present utility model, those skilled in the art are not having
The every other embodiment obtained on the premise of making creative work, belongs to the scope of the utility model protection.
Embodiment
As shown in Figure 1 and Figure 2, a kind of 96 orifice plates of suitable protein precipitation, including main pipe 1, the bottom of the main pipe 1
An opening is offered, the auxiliary pipe storehouse 2 being seamlessly connected with main pipe 1 is provided with the opening, make auxiliary pipe storehouse 2 with the phase of main pipe 1
Connection arrangement, elastic packing door 3 is provided with the opening that the auxiliary pipe storehouse 2 is connected with the main pipe 1, makes auxiliary pipe storehouse
2 with the spaced apart of main pipe 1, when the albumen gravity in main pipe 1 more than elastic packing door 3 it is elastic after, elastic packing storehouse
Door 3 opens arrangement to the inwall direction in auxiliary pipe storehouse 2.By in the bottom of main pipe 1 opening up opening and setting on the lower end of opening
Auxiliary pipe storehouse 2 is equipped with, while effectively can be separated albumen after installing elastic packing door 3 therebetween, is hardly subject to
Siphon produces the influence of current, and particle is difficult floating, and imbibition volume is more accurate.
Auxiliary pipe storehouse 2 described in the utility model and main pipe 1 are formed in one arrangement, it is ensured that 1 liang of auxiliary pipe storehouse 2 and main pipe
The sealing of person.
The opening of elastic packing door 3 described in the utility model is yi word pattern, or for cross, using it is any
Can, as long as elastic packing door 3 can be met when in use, protein body and liquid can be separated.
The material of elastic packing door described in the utility model 3 is soft silica gel, or is thin polymer film, is generally used soft
Silica gel, its service life is relatively long, can reduce its cost.
Elastic packing door described in the utility model 3 is socketed on the opening of the bottom of the main pipe 1, in main pipe
1 equipped with liquid when, elastic packing door 3 is convenient to elastic packing storehouse by main pipe 1 and the auxiliary exclusion seal of pipe storehouse 2 cut-off arrangement
The replacing of door 3, it is ensured that its sealing.
The shape in auxiliary pipe storehouse 2 described in the utility model needs to be selected in cylinder or cone arrangement, foundation
Select.
Operation principle of the present utility model is as follows：
Elastic packing door 3 is closed during the sample-adding, reagent adding and the vortex that process before the protein precipitation.And
During centrifugation, the protein body and extract solution of denaturation push up out elastic packing door 3 under the influence of centrifugal force, into auxiliary pipe storehouse 2.
Protein body is concentrated in the narrow auxiliary pipe storehouse 2 in bottom, and during extraction fluid, pipette is not easy to be drawn onto the albumen of bottom
Grain.
Embodiment 1
100 microlitres of plasma samples are extracted, selects 96 orifice plates of 50 microlitres of auxiliary capacity of pipe storehouse 2 to be extracted.First add 100
Microlitre blood plasma, 400 microlitres of methyl alcohol are added, 96 orifice plates are placed in eddy oscillating 5min on rocker device, the albumen in plasma sample
Particle is formed after being fully denatured through methyl alcohol.By the way that after centrifugation, protein body volume is about 20 microlitres and is entered by elastic packing door 3
Enter in 50 microlitres of auxiliary pipe storehouses 2, so as to produce separation spatially with the extract solution in container.When pipetting extract solution, in pipette
Sample is limpid, without particulate matter, be adapted to subsequent analysis detection work.
Embodiment 2
300 microlitres of plasma samples are extracted, selects 96 orifice plates of 100 microlitres of auxiliary capacity of pipe storehouse 2 to be extracted, first added
300 microlitres of blood plasma, 1mL methyl alcohol is added, 96 orifice plates are placed in eddy oscillating 5min on rocker device.Albumen warp in plasma sample
Methyl alcohol forms particle after being fully denatured.By the way that after centrifugation, protein body volume is about 60 microlitres and is entered by elastic packing door 3
In 100 microlitres of auxiliary pipe storehouses 2, so as to produce separation spatially with the extract solution in container.When pipetting extract solution, sample in pipette
Product are limpid, without particulate matter, be adapted to subsequent analysis detection work.
The utility model pipette in pipetting processes is difficult to touch precipitation particle, in addition particle be in auxiliary pipe storehouse in and
Liquid in main pipe has separation spatially, is hardly influenceed by siphon generation current, and particle is difficult floating.Imbibition body
Product is more accurate, parallel.Sample cleanliness factor is greatly improved, hardly has the extract solution that protein body shape material is present in after separating
In, it is not easily blocked analytical instrument.
The above is only preferred embodiment of the present utility model, is not limited to the utility model, it is noted that
For those skilled in the art, on the premise of the utility model know-why is not departed from, can also do
Go out some improvement and modification, these are improved and modification also should be regarded as protection domain of the present utility model.
Claims (6)
1. a kind of 96 orifice plates of suitable protein precipitation, it is characterised in that：Including main pipe (1), the bottom of the main pipe (1) is opened
An opening is provided with, the auxiliary pipe storehouse (2) being seamlessly connected with main pipe (1) is provided with the opening, make auxiliary pipe storehouse (2) and main pipe
(1) be connected arrangement, and elastic packing door is provided with the opening that the auxiliary pipe storehouse (2) is connected with the main pipe (1)
(3) auxiliary pipe storehouse (2) and main pipe (1) spaced apart, are made, when the liquid in main pipe (1) and centrifugal force suffered by protein body are big
In after the elasticity of elastic packing door (3), elastic packing door (3) opens arrangement to the inwall direction of auxiliary pipe storehouse (2).
2. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The auxiliary pipe storehouse (2) and supervisor
Body (1) is formed in one arrangement.
3. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The elastic packing door (3)
Opening be yi word pattern, or for cross.
4. 96 orifice plates of suitable protein precipitation according to claim 3, it is characterised in that：The elastic packing door (3)
Material be soft silica gel, or be thin polymer film.
5. 96 orifice plates of suitable protein precipitation according to claim 4, it is characterised in that：The elastic packing door (3)
It is socketed on the opening of the bottom of the main pipe (1), when main pipe (1) is equipped with liquid, elastic packing door (3) will be responsible for
Body (1) separates with auxiliary pipe storehouse (2) exclusion seal to be arranged.
6. 96 orifice plates of suitable protein precipitation according to claim 1, it is characterised in that：The shape of the auxiliary pipe storehouse (2)
In cylinder or cone arrangement.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621287361.XU CN206223520U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable protein precipitation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621287361.XU CN206223520U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable protein precipitation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206223520U true CN206223520U (en) | 2017-06-06 |
Family
ID=58786464
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621287361.XU Active CN206223520U (en) | 2016-11-28 | 2016-11-28 | A kind of 96 orifice plates of suitable protein precipitation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206223520U (en) |
-
2016
- 2016-11-28 CN CN201621287361.XU patent/CN206223520U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yang Yong Inventor after: Zhong Kan Inventor after: Liu Xuling Inventor after: Wen Fengjiao Inventor after: Zuo Zhenglong Inventor after: Niu Xiaoying Inventor after: Xiao Tao Inventor before: Yang Yong |
|
| CB03 | Change of inventor or designer information |