JP6344299B2 - Pretreatment device - Google Patents

Description

  The present invention relates to a pretreatment apparatus for performing pretreatment by dispensing a sample or a reagent with a probe.

  For example, when analyzing components contained in a sample derived from a living body such as whole blood, serum, filter paper blood, and urine, the sample may be analyzed after being pretreated by a pretreatment device. Examples of the pretreatment include a process of removing a specific component unnecessary for analysis from a sample and extracting a necessary component, and a process of concentrating or drying the extracted sample. Conventionally, various configurations have been proposed as preprocessing devices that automatically execute such preprocessing (see, for example, Patent Document 1 below).

  The sample is mixed with a reagent as necessary. Samples and reagents are stored in a plurality of containers little by little, and the sample or reagent is aspirated and dispensed from each container by a probe. Since the sample after dispensing is in a state where the sample or reagent is attached, the next dispensing is performed after washing with the washing liquid in the washing pot (washing unit).

  Waste liquid (cleaning liquid) after washing the probe is collected in a waste liquid tank. The waste liquid from which the sample has been washed out and the waste liquid from which the reagent has been washed out flow into the waste liquid tank through a common buffer section. The buffer section is provided with an inflow port from which the waste liquid flows from the washing pot and an outflow port from which the waste liquid flows out to the waste liquid tank. These inflow port and outflow port are connected via a buffer space. Yes.

  As a result, the waste liquid that has flowed into the buffer portion via the inflow port is guided to the outflow port through the buffer space, and is discharged from the outflow port to the waste liquid tank. When the waste liquid is collected up to a certain amount in the waste liquid tank, the analyst can remove the waste liquid in the waste liquid tank by taking out the waste liquid tank from the pretreatment device.

JP 2010-60474 A

  However, in the conventional configuration as described above, the outlet may be clogged in the buffer unit. That is, in the buffer space into which the waste liquid flows after cleaning the probe, there is a problem that the deposits are likely to be clogged at the outlet by gradually increasing the deposits over a long period of time.

  In particular, if the waste liquid from which samples containing serum and plasma components are washed out and the waste liquid from which reagents comprising organic solvents such as methanol and acetonitrile are washed out into the common buffer space, the samples in the waste liquid In addition, there is a high possibility that the reagent is mixed and insoluble matter is generated, and the insoluble matter is deposited and the outlet is clogged. If the outlet is clogged in the buffer section, the waste liquid may flow back to the washing pot through the piping and leak out, which may contaminate the examination room.

  The present invention has been made in view of the above circumstances, and an object of the present invention is to provide a pretreatment device that can satisfactorily guide the waste liquid to the waste liquid tank even when the outlet is clogged in the buffer section.

  The pretreatment apparatus according to the present invention includes a probe, a cleaning unit, a buffer unit, and a waste liquid tank. The probe sucks and dispenses a sample or a reagent. The cleaning unit cleans the probe with a cleaning liquid. Waste liquid from the cleaning unit flows into the buffer unit. The waste liquid tank stores the waste liquid guided through the buffer unit.

  The buffer portion is formed with an inlet, an outlet, a buffer space, and an overflow outlet. The waste liquid from the cleaning unit flows into the inlet. From the outlet, the waste liquid flows out to the waste liquid tank. The buffer space guides waste liquid flowing in from the inlet to the outlet. The overflow outlet is provided at a position higher than the outlet in the buffer space, and guides waste liquid overflowing from the buffer space to the waste liquid tank.

  According to such a configuration, even when the outlet is clogged in the buffer unit, the waste liquid can be overflowed from the overflow outlet provided at a position higher than the outlet in the buffer space. Since the waste liquid overflowed from the overflow outlet can be guided well to the waste liquid tank, it is possible to prevent the waste liquid from flowing backward and leaking.

  The inflow port and the outflow port may be formed on opposite sides of the buffer space. In this case, the overflow outlet may be provided on the inlet side with respect to the buffer space.

  According to such a configuration, the waste liquid flowing into the buffer space from the inflow port is favorably guided to the outflow port formed on the opposite side of the inflow port across the buffer space. On the other hand, since the overflow outlet is provided on the inlet side with respect to the buffer space, the waste liquid flowing into the buffer space from the inlet is prevented from overflowing from the overflow outlet when the outlet is not clogged. can do.

  The buffer section includes a first opening provided on the inlet side with respect to the buffer space, a second opening provided on the outlet side with respect to the buffer space, the first opening, and the A through hole communicating with the second opening may be formed. In this case, the pretreatment device connects a first pipe connecting the overflow outlet to the first opening, a second pipe connecting the second opening to the waste liquid tank, and connects the outlet to the waste liquid tank. You may further provide the 3rd piping to do.

  According to such a configuration, the waste liquid overflowing from the overflow outlet is guided to the waste liquid tank via the first pipe, the first opening, the through hole, the second opening, and the second pipe. Since the first opening is provided on the inflow side with respect to the buffer space, similarly to the overflow outlet, the first pipe connecting the overflow outlet and the first opening is prevented from forming a complicated flow path. In addition, it is possible to make the first pipe difficult to clog.

  Further, since the second opening is provided on the outlet side with respect to the buffer space, a second pipe that connects the second opening to the waste liquid tank, and a third pipe that connects the outlet to the waste liquid tank, They can be arranged close to each other and connected to the waste liquid tank through short channels. Thereby, it can prevent that 2nd piping and 3rd piping comprise a complicated flow path, it can make these piping hard to clog, and can perform maintenance with respect to these piping easily.

  The pretreatment device may further include a junction pipe that joins the second pipe and the third pipe to connect to the waste liquid tank.

  According to such a configuration, the second pipe and the third pipe can be connected to the waste liquid tank at once by the merge pipe. Therefore, when the waste liquid in the waste liquid tank is discarded, the second pipe and the third pipe can be attached to and detached from the waste liquid tank at a time, so that maintenance is further facilitated.

  At least one of the first pipe, the second pipe, and the third pipe may be transparent or translucent.

  According to such a configuration, the inside of the transparent or translucent pipe can be visually confirmed to easily check whether or not the pipe is clogged. In particular, since the second pipe and the third pipe are arranged close to each other, if these pipes are made transparent or translucent, it can be easily confirmed at once whether these pipes are not clogged.

  At least one of the first pipe, the second pipe, and the third pipe may be provided with a blockage preventing member that prevents the internal flow path from being blocked.

  According to such a structure, it can prevent that the flow path in piping is obstruct | occluded by the obstruction | occlusion prevention member. Thereby, it is possible to prevent the waste liquid from flowing back and leaking due to the blockage of the flow path in the pipe. In particular, the second pipe and the third pipe have many opportunities to be deformed because they are attached to and detached from the waste liquid tank when the waste liquid in the waste liquid tank is discarded. It is possible to effectively prevent the channel from being blocked.

  The buffer portion may be arranged such that an upper portion thereof is inclined toward the outlet side.

  According to such a configuration, by arranging the buffer portion to be inclined, even if both the outlet and the overflow outlet are clogged, the waste liquid overflows from the upper portion of the buffer portion to the outlet side. Can do. As a result, the waste liquid can be prevented from flowing back, and the waste liquid overflowing from the upper part of the buffer section can be dropped onto the rack of the waste liquid tank provided on the outlet side, so that the waste liquid is pretreated. It is possible to effectively prevent leakage to the outside of the apparatus.

  The pretreatment apparatus may further include an inflow pipe that connects the cleaning unit to the inflow port, and a branch pipe that branches from the inflow pipe and communicates with the first pipe.

  According to such a configuration, even when the inflow port is clogged instead of the outflow port, the waste liquid is guided to the first pipe through the branch pipe branched from the inflow pipe. As a result, since the waste liquid can be guided to the waste liquid tank through the common flow path with the waste liquid overflowing from the overflow outlet, it is possible to prevent the waste liquid from flowing back and leaking while preventing the flow path from becoming complicated. Further, it can be effectively prevented.

  The probe may include a sample dispensing probe that sucks and dispenses a sample, and a reagent dispensing probe that sucks and dispenses a reagent. In this case, the inflow port may include a first inflow port through which waste liquid from the cleaning of the sample dispensing probe flows and a second inflow port through which waste liquid from the cleaning of the reagent dispensing probe flows. Good.

  According to such a configuration, the waste liquid from which the sample has been washed away from the sample dispensing probe and the waste liquid from which the reagent has been washed away from the reagent dispensing probe flow into the buffer space via different inlets. As a result, it is possible to prevent the sample and the reagent in the waste liquid from being mixed to generate an insoluble matter, so that the inflow port can be prevented from being clogged due to the accumulation of the insoluble matter.

  The first inflow port may be provided at a position that is higher than the second inflow port and in which waste liquid flowing into the buffer space from the first inflow port does not contact the second inflow port.

  According to such a configuration, when the waste liquid from which the sample has been washed out from the sample dispensing probe flows into the buffer space from the first inlet, the waste liquid falls in the buffer space and comes into contact with the second inlet. Can be prevented. Thereby, it is possible to prevent the sample and the reagent in the waste liquid from being mixed with each other at the second inflow port, thereby preventing the insoluble matter from being generated. Therefore, the second inflow port can be prevented from being clogged with the insoluble matter.

  According to the present invention, even when the outflow port is clogged in the buffer unit, the waste liquid can be overflowed from the overflow outlet provided at a position higher than the outflow port in the buffer space, and the waste liquid can be guided well to the waste liquid tank.

It is a schematic front view which shows the structural example of the analysis system provided with the pre-processing apparatus which concerns on one Embodiment of this invention. It is a top view which shows the structural example of a pre-processing apparatus. It is a side view which shows the structural example of a separation container. It is a top view of the separation container of FIG. 3A. It is sectional drawing which shows the AA cross section of FIG. 3B. It is a side view which shows the structural example of a collection container. It is a top view of the collection container of Drawing 4A. It is sectional drawing which shows the BB cross section of FIG. 4B. It is sectional drawing which shows the pre-processing kit of the state with which the separation container and the collection container were piled up. It is a top view which shows the structural example of a filtration port. It is sectional drawing which shows the XX cross section of FIG. 6A. It is sectional drawing which shows the YY cross section of FIG. 6A. It is sectional drawing which shows the state which installed the pre-processing kit in the filtration port. It is the schematic which shows the structural example of a negative pressure load mechanism. It is the schematic for demonstrating the flow path of a waste liquid. It is the schematic plan view which showed the structural example of the vicinity of a buffer part. It is the schematic front view which showed the structural example of the vicinity of a buffer part. It is a schematic sectional drawing of a buffer part.

  FIG. 1 is a schematic front view illustrating a configuration example of an analysis system including a pretreatment device 1 according to an embodiment of the present invention. This analysis system includes a pretreatment device 1, an LC (liquid chromatograph) 100, and an MS (mass spectrometry device) 200. Samples that have been pretreated by the pretreatment device 1 are sequentially introduced into the LC100 and the MS200. Analysis.

  That is, the analysis system according to the present embodiment has a configuration in which a liquid chromatograph mass spectrometer (LC / MS) is connected to the pretreatment device 1. However, the present invention is not limited to this configuration, and either one of the LC 100 and the MS 200 is omitted, so that the sample subjected to the pre-processing by the pre-processing apparatus 1 is introduced only into either the LC 100 or the MS 200. Such a configuration may be adopted.

  The pretreatment device 1 functions as a dispensing device that dispenses a sample such as plasma, serum, or an aqueous caffeine solution, or a reagent mixed with the sample to a dispensing position, and is dispensed to the dispensing position. Various pretreatments such as filtration, stirring and temperature control are performed on the sample and the reagent. The sample after these pretreatments are introduced into the LC 100 via the autosampler 101 provided in the LC 100.

  The LC 100 includes a column (not shown), and sample components separated in the process of passing the sample through the column are sequentially introduced into the MS 200. The MS 200 includes an ionization unit 201 that ionizes a sample introduced from the LC 100 and a mass analysis unit 202 that analyzes the ionized sample.

  The preprocessing device 1 is provided with an operation display unit 1a including a touch panel, for example. The analyst can perform input related to the operation of the preprocessing device 1 by operating the display screen of the operation display unit 1a, and can also provide information regarding the operation of the preprocessing device 1 displayed on the display screen of the operation display unit 1a. Can be confirmed. However, the configuration is not limited to the configuration in which the touch panel type operation display unit 1a is provided. For example, the display unit configured by a liquid crystal display and the operation unit configured by operation keys or the like are provided separately. May be.

  FIG. 2 is a plan view showing a configuration example of the pretreatment device 1. In this pretreatment apparatus 1, a pretreatment kit consisting of a set of a separation container 50 and a collection container 54 is used for each sample, and pretreatment (filtering, stirring, temperature control, etc.) set for each pretreatment kit is used. ) Is executed.

  In addition to the dispensing port 32 serving as a dispensing position, the pretreatment device 1 is provided with a plurality of processing ports for performing pretreatment on the sample dispensed to the dispensing port 32. Thereby, by installing the pretreatment kit containing the sample in any of the processing ports, the pretreatment corresponding to each processing port is executed on the sample contained in the pretreatment kit. It has become.

  As processing ports, a filtration port 30, a disposal port 34, an agitation port 36a, temperature control ports 38 and 40, a transfer port 43, a washing port 45, and the like are provided in association with each pretreatment. Each of these processing ports constitutes a plurality of preprocessing units that respectively execute a plurality of types of preprocessing.

  The separation container 50 and the collection container 54 constituting the pretreatment kit are transported between the processing ports by the transport arm 24 as a transport unit. A holding portion 25 for holding the separation container 50 and the collection container 54 is formed on the distal end side of the transfer arm 24. The proximal end side of the transfer arm 24 is held so as to be rotatable about the vertical shaft 29. The transfer arm 24 extends in the horizontal direction, and rotates around the vertical axis 29 to move the holding unit 25 so as to draw an arcuate trajectory in the horizontal plane. Each processing port, which is the transfer destination of the separation container 50 and the collection container 54, and other ports are all provided on an arc-shaped track drawn by the holding unit 25.

  A sample is dispensed from the sample container 6 into the pretreatment kit. A plurality of sample containers 6 in which samples are stored can be installed in the sample installation unit 2, and samples are sequentially collected from each sample container 6 using the sample dispensing arm 20. A plurality of sample racks 4 that hold a plurality of sample containers 6 are arranged in a ring shape in the sample setting unit 2. The sample installation part 2 moves each sample rack 4 in the circumferential direction by rotating in a horizontal plane. Thereby, each sample container 6 can be sequentially moved to a predetermined sample collection position.

  Here, the sample collection position is located on the trajectory of the sample dispensing probe 20a provided at the tip of the sample dispensing arm 20, and the sample is removed from the sample container 6 by the sample dispensing probe 20a at the sample collection position. Is sucked. The sample in the sample container 6 is aspirated by the sample dispensing probe 20 a and then dispensed by being discharged to the separation container 50 installed in the dispensing port 32.

  The sample dispensing arm 20 can rotate in a horizontal plane around a vertical shaft 22 provided on the base end side, and can move up and down in the vertical direction along the vertical shaft 22. The sample dispensing probe 20 a is held so as to extend vertically downward at the tip of the sample dispensing arm 20, and moves to draw an arc-shaped trajectory in the horizontal plane according to the operation of the sample dispensing arm 20. Alternatively, vertical movement in the vertical direction is performed.

  The dispensing port 32 is provided at a position on the track of the sample dispensing probe 20 a and on the track of the holding unit 25 of the transport arm 24. The dispensing port 32 is a port for dispensing a sample from the sample dispensing probe 20a to an unused separation container 50. The unused separation container 50 is transported to the dispensing port 32 by the transport arm 24.

  A reagent installation unit 8 for installing the reagent container 10 is provided at the center of the sample installation unit 2 in which the sample racks 4 are arranged in an annular shape. The reagent in the reagent container 10 installed in the reagent installing unit 8 is collected using the reagent dispensing arm 26. The base end of the reagent dispensing arm 26 is supported by a vertical shaft 29 that is common to the transfer arm 24, and can be rotated in a horizontal plane around the vertical shaft 29, and vertically along the vertical shaft 29. Can move up and down in the direction.

  A reagent dispensing probe 26 a is held at the tip of the reagent dispensing arm 26 so as to extend vertically downward. The reagent dispensing probe 26a is moved in the horizontal plane to draw the same arc-shaped trajectory as the holding unit 25 of the transfer arm 24 or vertically moved in accordance with the operation of the reagent dispensing arm 26.

  The reagent installing unit 8 is rotatable in a horizontal plane independently of the sample installing unit 2. A plurality of reagent containers 10 are arranged in an annular shape in the reagent installing unit 8, and each reagent container 10 moves in the circumferential direction as the reagent installing unit 8 rotates. Thereby, the desired reagent container 10 can be moved to a predetermined reagent collection position.

  Here, the reagent collection position is located on the trajectory of the reagent dispensing probe 26a provided at the tip of the reagent dispensing arm 26, and the reagent dispensing probe 26a at the reagent collecting position is used to remove the reagent from the reagent container 10. Is sucked. The reagent in the reagent container 10 is aspirated by the reagent dispensing probe 26 a and then dispensed by being discharged to the separation container 50 installed in the dispensing port 32, so that the sample in the separation container 50 is dispensed. Added.

  The separation container 50 and the recovery container 54 are held by a container holding unit 12 provided at a position different from the sample setting unit 2 and the reagent setting unit 8. In the container holding unit 12, a plurality of sets of pretreatment kits in which unused separation containers 50 and recovery containers 54 are stacked are arranged in an annular shape. The container holding unit 12 includes a rotating unit 14 that rotates in a horizontal plane, and a plurality of container racks 16 that can be attached to and detached from the rotating unit 14.

  Each container rack 16 can hold a plurality of pretreatment kits. The plurality of container racks 16 are arranged on the rotating unit 14 in an annular shape. An annular holding region for holding a plurality of pretreatment kits is formed by the plurality of container racks 16 arranged side by side in an annular shape. The rotating unit 14 displaces each container rack 16 in the circumferential direction of the holding region by rotating in a horizontal plane. Thereby, a plurality of pretreatment kits can be sequentially moved to a predetermined transport position. Here, the transfer position is located on the track of the holding unit 25 provided at the tip of the transfer arm 24, and the separation container 50 or the collection container 54 is held by the holding unit 25 at the transfer position, and the transfer destination To the next port.

  In this manner, by dividing the container rack 16 into a plurality of container racks 16 and holding the pretreatment kit, each container rack 16 can be individually attached to and detached from the rotating unit 14. As a result, even when the separation container 50 or the collection container 54 held in any one of the container racks 16 is being processed, the other container rack 16 can be attached and detached to perform another operation. Therefore, the preprocessing efficiency can be improved.

  However, the separation container 50 and the collection container 54 are not limited to the configuration in which the separation container 50 and the collection container 54 are held by the container holding unit 12 via the container rack 16, and may be configured to be directly held by the container holding unit 12, for example. . Further, the separation container 50 and the collection container 54 are not limited to the structure in which the separation container 50 and the collection container 54 are held by the container holding unit 12 in a state of being overlapped with each other. There may be. Further, the plurality of container racks 16 is not limited to a configuration in which the plurality of container racks 16 are arranged in an annular shape, and may have a configuration in which the plurality of container racks 16 are arranged in an arc shape, for example. In this case, a plurality of separation containers 50 and recovery containers 54 are held in an arc-shaped holding area instead of an annular shape.

  In the container holding part 12, an analyst can install a plurality of types (for example, two types) of separation containers 50 provided with separation layers having different separation performances. These separation containers 50 are selectively used according to the analysis item of the sample, and the separation container 50 corresponding to the analysis item designated by the analyst is selected from the container holding unit 12 and conveyed. Here, the analysis item is a type of analysis that is subsequently performed using the sample that has been pre-processed by the pre-processing apparatus 1, for example, a type of analysis that is performed by the LC 100 or the MS 200.

  FIG. 3A is a side view showing a configuration example of the separation container 50. FIG. 3B is a plan view of the separation container 50 of FIG. 3A. FIG. 3C is a cross-sectional view showing the AA cross section of FIG. 3B. FIG. 4A is a side view showing a configuration example of the collection container 54. 4B is a plan view of the collection container 54 of FIG. 4A. FIG. 4C is a cross-sectional view showing a BB cross section of FIG. 4B. FIG. 5 is a cross-sectional view showing the pretreatment kit in a state where the separation container 50 and the collection container 54 are overlaid.

  As illustrated in FIGS. 3A to 3C, the separation container 50 is a cylindrical container having an internal space 50 a that stores a sample and a reagent. A separation layer 52 is provided at the bottom of the internal space 50a. The separation layer 52 is a separation agent or a separation membrane having a function of selectively separating a specific component in a sample by allowing the sample to pass through and reacting with the specific component physically or chemically, for example.

  As a separating agent constituting the separating layer 52, for example, ion exchange resin, silica gel, cellulose, activated carbon, or the like can be used. Examples of the separation membrane include PTFE (polytetrafluoroethylene) membrane, nylon membrane, polypropylene membrane, PVDF (polyvinylidene fluoride) membrane, acrylic copolymer membrane, mixed cellulose membrane, nitrocellulose membrane, polyethersulfone membrane, An ion exchange membrane, a glass fiber membrane, etc. can be used.

  As a deproteinization filter (separation membrane) for removing the protein in the sample by filtration, PTFE, an acrylic copolymer membrane, or the like can be used. In this case, a prefilter (not shown) may be provided above the separation layer 52 in order to prevent clogging of the deproteinization filter. As such a prefilter, for example, a nylon film, a polypropylene film, a glass fiber film, or the like can be used. The prefilter is for removing insoluble substances and foreign matters having a relatively large particle diameter from the sample. This prefilter can prevent the deproteinization filter from being clogged with insoluble substances and foreign matters having a relatively large particle size.

  On the upper surface of the separation container 50, an opening 50b for injecting a sample or a reagent is formed. Further, an extraction port 50 d for extracting the sample that has passed through the separation layer 52 is formed on the lower surface of the separation container 50. On the upper part of the outer peripheral surface of the separation container 50, a flange portion 50c for engaging the holding portion 25 of the transfer arm 24 is formed so as to protrude in the circumferential direction.

  A skirt portion 51 that contacts the edge of the filtration port 30 when the separation container 50 is accommodated in the filtration port 30 together with the collection container 54 is provided at the center of the outer peripheral surface of the separation container 50. The skirt portion 51 protrudes in the circumferential direction from the outer peripheral surface of the separation container 50 and is formed in an L-shaped cross section so as to extend downward therefrom, thereby forming a certain space between the outer peripheral surface of the separation container 50. doing.

  As shown in FIGS. 4A to 4C and 5, the collection container 54 is a bottomed cylindrical container that houses the lower part of the separation container 50 and collects the sample extracted from the extraction port 50 d of the separation container 50. . An opening 54 b for inserting the lower part of the separation container 50 is formed on the upper surface of the collection container 54. Inside the collection container 54, an internal space 54 a that accommodates a portion of the separation container 50 below the skirt portion 51 is formed. Similar to the separation container 50, a flange part 54 c for engaging the holding part 25 of the transfer arm 24 is formed on the upper part of the outer peripheral surface of the collection container 54 so as to protrude in the circumferential direction.

  In the state where the separation container 50 and the collection container 54 are overlapped as shown in FIG. 5, the upper part of the collection container 54 enters the inside of the skirt portion 51. The outer diameter of the separation container 50 is smaller than the inner diameter of the collection container 54. Thereby, a slight gap is formed between the outer peripheral surface of the separation container 50 accommodated in the internal space 54 a of the recovery container 54 and the inner peripheral surface of the recovery container 54. In the container holding unit 12, the separation container 50 and the recovery container 54 are installed in a state where the lower part of the separation container 50 is accommodated in the recovery container 54 (state in FIG. 5).

  Three cutouts 54 d are formed on the edge of the upper surface of the collection container 54. Therefore, the separation container 50 and the collection container 54 are overlapped as shown in FIG. 5 so that the collection container 54 can be collected through the notch 54 even when the upper surface of the collection container 54 is in contact with the inner surface of the skirt portion 51. The inside and outside of the container 54 can be communicated. However, the number of notches 54d is not limited to three, and may be two or less, or four or more. Moreover, not only the notch 54d but the structure in which the small hole was formed may be sufficient, for example.

  Referring to FIG. 2 again, the filtration port 30 is provided inside the container holding part 12. That is, an annular or arc-shaped holding region is formed by the plurality of container racks 16 arranged side by side on the outer periphery of the filtration port 30, and the plurality of separation containers 50 and the collection containers 54 are held in the holding region. Yes. As described above, the holding region of the separation container 50 and the recovery container 54 is formed in an annular shape or an arc shape, and the installation space for the filtration port 30 is secured in the empty space in the center portion, thereby making the configuration more compact. Can do.

  In particular, in this embodiment, since the separation container 50 and the collection container 54 are held in the holding region in a stacked state, it is not necessary to provide separate holding regions for the separation container 50 and the collection container 54. Therefore, more separation containers 50 and recovery containers 54 can be held in a small holding area. Thereby, the holding | maintenance area | region of the separation container 50 and the collection | recovery container 54 can be made small, and it can be set as a more compact structure.

  Further, by providing the filtration port 30 at the center of the holding area formed in an annular shape or an arc shape, the distance between the plurality of separation containers 50 and the collection containers 54 held in the holding area and the filtration port 30 can be relatively increased. Can be shortened. Thereby, since the time which conveys the separation container 50 and the collection | recovery container 54 to the filtration port 30 can be shortened, pre-processing efficiency can be improved.

  The filtration port 30 constitutes a filtration unit that separates the sample by the separation layer 52 by applying pressure to the sample in the separation container 50. In the present embodiment, for example, two filtration ports 30 are provided side by side on the track of the holding unit 25 of the transport arm 24. The separation container 50 and the collection container 54 are installed in each filtration port 30 in a state of being overlapped as shown in FIG. 5, and the sample separated by the separation layer 52 in the separation container 50 by the negative pressure is contained in the collection container 54. It has come to be collected. However, the separation container 50 and the collection container 54 are not limited to the structure in which the separation container 50 and the collection container 54 are installed in each filtration port 30 in a state of being overlapped with each other, but the structure in which the separation container 50 and the collection container 54 are individually installed. There may be. Further, the number of filtration ports 30 is not limited to two, and may be one or three or more.

  Three agitation ports 36 a are provided in the agitation unit 36 provided in the vicinity of the container holding unit 12, for example, on the track of the holding unit 25 of the transfer arm 24. The stirring unit 36 has a mechanism for individually operating each stirring port 36a in a horizontal plane. By such a mechanism, the sample in the separation container 50 arranged in each stirring port 36a can be stirred. However, the number of stirring ports 36a is not limited to three, and may be two or less, or four or more.

  The temperature control ports 38 and 40 are provided, for example, in a thermally conductive block whose temperature is controlled by a heater and a Peltier element, and the temperature of the separation container 50 or the recovery container 54 accommodated in the temperature control ports 38 and 40 is constant. Adjusted to temperature. The temperature control port 38 is for the separation container 50, and, for example, four temperature adjustment ports 38 are arranged side by side on the track of the holding unit 25 of the transfer arm 24. The temperature control port 40 is for the recovery container 54, and four temperature control ports 40 are arranged side by side on the track of the holding portion 25 of the transfer arm 24, for example, similarly to the temperature control port 38 for the separation container 50. However, the number of temperature control ports 38 and 40 is not limited to four, but may be three or less, or may be five or more.

  FIG. 6A is a plan view illustrating a configuration example of the filtration port 30. FIG. 6B is a cross-sectional view showing the XX cross section of FIG. 6A. 6C is a cross-sectional view showing the YY cross section of FIG. 6A. FIG. 6D is a cross-sectional view showing a state where the pretreatment kit is installed in the filtration port 30.

  The filtration port 30 is formed of, for example, a recess, and the recess constitutes an installation space 30a for installing the pretreatment kit. That is, as shown in FIG. 6D, the separation container 50 and the collection container 54 conveyed from the container holding unit 12 by the conveyance arm 24 are installed in the installation space 30a so as to overlap each other. At this time, the collection container 54 is first accommodated in the installation space 30a, and then the lower part of the separation container 50 is accommodated in the internal space 54a of the recovery container 54.

  A holding member 31 that holds the collection container 54 so as to sandwich the collection container 54 is provided in the filtration port 30. The holding member 31 is, for example, a U-shaped metal member that is open at the top, and constitutes two leaf springs in which two arms extending upward can be elastically displaced in the inner diameter direction of the filtration port 30. doing. The two leaf spring portions of the holding member 31 have, for example, a curved shape or a bent shape that is recessed inward so that the interval between the upper end portion and the lower end portion is the narrowest. The distance between the two leaf spring portions is larger than the outer diameter of the recovery container 54 at the upper end and the lower end, and smaller than the outer diameter of the recovery container 54 at the narrowest distance.

  Due to the shape of the holding member 31 as described above, when the collection container 54 is inserted into the installation space 30a of the filtration port 30, the two leaf springs of the holding member 31 are lowered as the collection container 54 descends. The portion is opened, and the collection container 54 is held in the installation space 30a by the elastic force. The collection container 54 is equally pressed from two opposing directions by the two leaf spring portions of the holding member 31 and is held in the central portion of the installation space 30a. The holding member 31 is fixed in the installation space 30a so that it does not float with the recovery container 54 when the recovery container 54 is taken out.

  A ring-shaped sealing member 60 having elasticity is provided at the edge of the upper surface opening of the filtration port 30. The sealing member 60 is fitted, for example, in a recess provided at the edge of the upper surface opening of the filtration port 30. The material of the sealing member 60 is an elastic material such as silicone rubber or EPDM (ethylene-propylene-diene rubber). When the collection container 54 and the separation container 50 are installed in the installation space 30 a of the filtration port 30, the lower end of the skirt portion 51 of the separation container 50 contacts the sealing member 60, and the installation space 30 a is formed by the skirt portion 51. It becomes a sealed state. However, the contact portion of the separation container 50 with the sealing member 60 is not limited to a member having a shape such as the skirt portion 51, and may be formed by contact portions having various other shapes such as a flange portion. Can do.

  A pressure reducing flow path 56 communicates with the installation space 30 a from the bottom surface of the filtration port 30. A flow path 57 of the negative pressure load mechanism 55 is connected to the flow path 56. The negative pressure load mechanism 55 includes a vacuum pump, for example, and constitutes a negative pressure load portion that loads a negative pressure into the installation space 30a. If the inside of the installation space 30a is decompressed by the negative pressure load mechanism 55 in a state where the separation container 50 and the collection container 54 are accommodated in the filtration port 30, the inside of the installation space 30a becomes negative pressure.

  In the installation space 30a that has become negative pressure, the internal space 54a of the recovery container 54 is formed through a notch 54d of the recovery container 54 and a gap between the inner peripheral surface of the recovery container 54 and the outer peripheral surface of the separation container 50. Communicate. Since the upper surface of the separation container 50 is open to the atmosphere, a pressure difference is generated between the internal space 50 a of the separation container 50 and the internal space 54 a of the recovery container 54 via the separation layer 52. Therefore, only the components that can pass through the separation layer 52 among the samples contained in the internal space 50a of the separation container 50 are separated by the separation layer 52 due to the pressure difference, and are moved to the internal space 54a side of the collection container 54. Extracted.

  FIG. 7 is a schematic diagram illustrating a configuration example of the negative pressure load mechanism 55. The two filtration ports 30 are connected to a common vacuum tank 66. Each filtration port 30 and the vacuum tank 66 are connected by a flow path 57, and a pressure sensor 62 and a three-way valve 64 are provided in each flow path 57. The pressure in the installation space 30 a of each filtration port 30 is detected by each pressure sensor 62. Each three-way valve 64 is in a state in which the filtration port 30 and the vacuum tank 66 are connected, in a state in which the filtration port 30 side of the flow path 57 is opened to the atmosphere (state in FIG. 7), or in the flow path 57 It can switch to either the state which sealed the edge part by the side of the filtration port 30. FIG.

  A pressure sensor 68 is connected to the vacuum tank 66, and a vacuum pump 58 is connected via a three-way valve 69. Therefore, by switching the three-way valve 69, the vacuum pump 58 can be connected to the vacuum tank 66 as needed, and the pressure in the vacuum tank 66 can be adjusted.

  When a sample extraction process is executed in any one of the filtration ports 30, a pressure sensor 62 is connected between the filtration port 30 and the vacuum tank 66 to detect the pressure in the installation space 30 a of the filtration port 30. The value of is adjusted to be a predetermined value. Thereafter, the end of the flow path 57 on the filtration port 30 side is sealed. Thereby, the installation space 30a of the filtration port 30 becomes a sealed system, and the sample is extracted by maintaining the reduced pressure state in the installation space 30a.

  Referring to FIG. 2 again, the pretreatment apparatus 1 is provided with a sample transfer unit 42 for transferring the sample extracted in the collection container 54 to the autosampler 101 side. The sample transfer unit 42 includes a moving unit 44 that moves in one direction (the arrow direction in FIG. 2) in the horizontal plane, and a transfer port 43 for installing the collection container 54 is provided on the upper surface of the moving unit 44. It has been. The moving unit 44 moves by the operation of a drive mechanism having a rack and pinion mechanism, for example.

  When the sample is not transferred to the autosampler 101 side, the transfer port 43 is arranged on the track of the holding unit 25 of the transfer arm 24 (position indicated by a solid line in FIG. 2). In this state, the collection container 54 is installed in the transfer port 43 by the transfer arm 24 and the collection container 54 is collected from the transfer port 43.

  When transferring the sample to the autosampler 101 side, after the collection container 54 containing the extracted sample is installed in the transfer port 43, the moving unit 44 moves to the outside of the pretreatment apparatus 1. The transfer port 43 is disposed at a position adjacent to the autosampler 101 (a position indicated by a broken line in FIG. 2). In this state, the sample in the collection container 54 is sucked by the sampling probe provided in the autosampler 101.

  When the sample suction by the autosampler 101 is completed, the moving unit 44 is returned to the original position (the position indicated by the solid line in FIG. 2), and the collection container 54 is collected by the transport arm 24. The used collection container 54 is transported to the disposal port 34 by the transport arm 24 and discarded. The disposal port 34 is disposed in the vicinity of the dispensing port 32 on the track of the holding unit 25 of the transfer arm 24, and the used separation container 50 and the collection container 54 are discarded.

  A cleaning port 45 for cleaning the sample dispensing probe 20a is provided on the trajectory of the sample dispensing probe 20a. A cleaning port 46 (see FIG. 8) for cleaning the reagent dispensing probe 26a is provided on the orbit of the reagent dispensing probe 26a. Waste liquid from the cleaning ports 45, 46 and the like is collected in a waste liquid tank 9 (see FIG. 8) through a flow path constituted by a plurality of pipes.

  FIG. 8 is a schematic diagram for explaining the flow path of the waste liquid. In the present embodiment, in addition to the cleaning port 45 for cleaning the sample dispensing probe 20a, the cleaning port 46 for cleaning the reagent dispensing probe 26a, the cold insulation unit 81 for cooling the reagent installing unit 8, Waste liquid from the dispensing port 32 or the like is collected in the waste liquid tank 9 via the common buffer unit 7.

  The cleaning ports 45 and 46 constitute a cleaning unit that cleans the probes 20a and 26a with a cleaning liquid such as pure water. At the time of cleaning the sample dispensing probe 20a, a cleaning liquid is supplied into the sample dispensing probe 20a from a cleaning liquid supply pump (not shown) while the sample dispensing probe 20a is inserted into the cleaning port 45, and the cleaning port 45 By discharging in, the sample dispensing probe 20a in the cleaning port 45 can be cleaned with the discharged cleaning liquid. Similarly, when cleaning the reagent dispensing probe 26a, a cleaning liquid is supplied into the reagent dispensing probe 26a from a cleaning liquid supply pump (not shown) while the reagent dispensing probe 26a is inserted into the cleaning port 46. By discharging into the cleaning port 46, the reagent dispensing probe 26a in the cleaning port 46 can be cleaned with the discharged cleaning liquid. Waste liquid from the cleaning ports 45 and 46 flows into the buffer unit 7 through the inflow pipes 451 and 461, respectively.

  From the cold insulation unit 81, water generated by condensation flows out as waste liquid, and the waste liquid flows into the buffer unit 7 through the inflow pipe 811. A pipe 321 joins the inflow pipe 811 via a junction pipe 810 provided in the middle of the inflow pipe 811. This pipe 321 is connected to the dispensing port 32. When a sample or reagent is accidentally discharged without the separation container 50 in the dispensing port 32, the sample or reagent is discharged as waste liquid to the pipe 321. It flows out, merges from the merge pipe 810 to the inflow pipe 811 and flows into the buffer section 7.

  A buffer space 71 is formed in the buffer unit 7. The buffer space 71 communicates with the inflow pipes 451, 461, 811 through different inflow ports 72, 73, 74. As a result, the waste liquid from the cleaning ports 45 and 46, the cold insulation unit 81 and the dispensing port 32 is guided to the inlets 72, 73 and 74 via the inflow pipes 451, 461 and 811. 73 and 74 flow into the buffer space 71. The waste liquid that has flowed into the buffer space 71 flows out from the outlet 75 and is guided to the waste liquid tank 9 through the pipe 91, thereby being stored in the waste liquid tank 9.

  As described above, the waste liquid from which the sample has been washed away from the sample dispensing probe 20a flows into the buffer space 71 via the inlet (first inlet) 72, and the waste liquid from which the reagent has been washed away from the reagent dispensing probe 26a is It flows into the buffer space 71 through an inlet (second inlet) 73 different from the inlet 72. Thereby, it is possible to prevent the insoluble matter from being generated by mixing the sample and the reagent in the waste liquid, so that the inflow ports 72 and 73 can be prevented from being clogged due to the accumulation of the insoluble matter.

  In the present embodiment, the inflow port 72 is provided at a position higher than the inflow port 73. Further, the inflow port 72 is provided at a position protruding into the buffer space 71 from the inflow port 73, so that the waste liquid flowing into the buffer space 71 from the inflow port 72 falls in the buffer space 71 and flows into the inflow port. 73 is prevented from touching.

  Accordingly, it is possible to prevent the sample and the reagent in the waste liquid from being mixed with each other at the inflow port 73 and thereby to generate an insoluble matter. Therefore, the inflow port 73 can be prevented from being clogged with the insoluble matter. In the present embodiment, the inflow port 74 is provided at a position that protrudes further into the buffer space 71 than the inflow port 72, so that the waste liquid flowing into the buffer space 71 from the inflow port 74 is flown into the inflow ports 72 and 73. It is supposed not to touch.

  The buffer space 71 has a larger cross-sectional area than the inflow ports 72, 73, 74 and the outflow port 75. Thereby, even when there is a large amount of waste liquid flowing in from the inlets 72, 73, 74, the waste liquid that has flowed in is temporarily stored in the buffer space 71 and guided to the outlet 75. The inflow ports 72, 73, and 74 are formed at positions higher than the outflow port 75 in the buffer space 71. An overflow outlet 76 is formed in the buffer space 71 at a position higher than the outlet 75 and lower than the inlets 72, 73, 74.

  In this embodiment, when the outflow port 75 is clogged, the waste liquid in the buffer space 71 can be overflowed from the overflow outlet 76 and guided to the pipe 92 connected to the overflow outlet 76. The pipe 92 is connected to a through hole 77 formed in the buffer unit 7, and the through hole 77 is connected to the waste liquid tank 9 through a pipe 93. As a result, the waste liquid overflowed from the overflow outlet 76 can be satisfactorily guided to the waste liquid tank 9 via the pipe 92, the through-hole 77 and the pipe 93. It is possible to prevent leakage from 32 and the like.

  The pipes 91 and 93 from the buffer unit 7 toward the waste liquid tank 9 are joined by a joining pipe 94 and connected to the waste liquid tank 9 at a time. Specifically, a junction pipe 94 is rotatably attached to a cap 95 for opening and closing the waste liquid tank 9. Thus, the analyst can attach and detach the cap 95 to and from the waste liquid tank 9 while preventing the pipes 91 and 93 from being entangled with each other by rotating the cap 95 while gripping the merging pipe 94 so as not to rotate. it can.

  By attaching and detaching the cap 95 in this manner, the operation of discarding the waste liquid in the waste liquid tank 9 can be easily performed. That is, when the waste liquid in the waste liquid tank 9 is discarded, the pipes 91 and 93 can be attached to and detached from the waste liquid tank 9 at a time by attaching and detaching the cap 95, so that maintenance is facilitated. The waste liquid tank 9 is held by, for example, a rack 90 slidable in the horizontal direction, and the waste liquid tank 9 can be maintained by pulling the rack 90 out of the pretreatment device 1.

  Pipes 453, 463, and 813 are connected to the inflow pipes 451, 461, and 811 through branch pipes 452, 462, and 812, respectively. These pipes 453, 463, and 813 are connected to a pipe 92 through which waste liquid overflowing from the buffer space 71 flows via a plurality of junction pipes 96, 97, and 98. That is, the inflow pipes 451, 461, and 811 are branched from the branch pipes 452, 462, and 812, respectively, and communicate with the common pipe 92. The branch pipes 452, 462, 812 and the pipes 453, 463, 813 are connected to the pipes 453, 453 of each branch so that the liquid that has passed through the inflow pipes 451, 461, 811 does not flow into the pipes 453, 463, 813. The 463 and 813 sides are held so as to be higher than the other branch side.

  Therefore, even when the inflow ports 72, 73, and 74 are clogged instead of the outflow port 75, the waste liquid is guided to the pipe 92 through the branch pipes 452, 462, and 812 branched from the inflow pipes 451, 461, and 811. Accordingly, since the waste liquid can be guided to the waste liquid tank 9 through the common pipe 92 with the waste liquid that overflows from the overflow outlet 76, the waste liquid flows backward and prevents the flow path from becoming complicated. It is possible to more effectively prevent leakage from 45, 46 and the dispensing port 32.

  FIG. 9 is a schematic plan view showing a configuration example in the vicinity of the buffer unit 7. FIG. 10 is a schematic front view showing a configuration example in the vicinity of the buffer unit 7. FIG. 11 is a schematic cross-sectional view of the buffer unit 7. The buffer unit 7 includes, for example, a main body 70 in which a buffer space 71 is formed, and a lid 78 that seals the buffer space 71 by closing an upper surface opening of the main body 70. Thereby, the deposits and the like in the buffer space 71 can be removed by attaching and detaching the lid 78 to the main body 70. The main body 70 is formed in a cylindrical shape, and is arranged such that the central axis L thereof is slightly inclined with respect to the vertical direction.

  Each inflow port 72, 73, 74 is formed on the opposite side of the outflow port 75 with the buffer space 71 interposed therebetween. As a result, the waste liquid flowing into the buffer space 71 from each of the inlets 72, 73, 74 is favorably transferred to the outlet 75 formed on the opposite side of each of the inlets 72, 73, 74 across the buffer space 71. Led.

  The overflow outlet 76 is formed on the opposite side of the outlet 75 with the buffer space 71 in between, like each of the inlets 72, 73 and 74. That is, since the overflow outlet 76 is provided on the respective inlets 72, 73, 74 side with respect to the buffer space 71, when the outlet 75 is not clogged, the buffer outlet 71 is connected to the buffer space 71. It is possible to prevent waste liquid flowing into the 71 from overflowing from the overflow outlet 76.

  The through-hole 77 includes a first opening 771 formed on each of the inflow ports 72, 73, 74 with respect to the buffer space 71 and a second opening 772 formed on the outflow port 75 side of the buffer space 71. It is formed below the buffer space 71 in the main body 70 of the buffer unit 7 so as to communicate with each other. The through-hole 77 extends in a straight line, for example, in a direction orthogonal to the central axis L of the main body 70.

  A pipe (first pipe) 92 communicating with the overflow outlet 76 is connected to the first opening 771. A pipe (second pipe) 93 communicating with the waste liquid tank 9 is connected to the second opening 772. Further, as described above, a pipe (third pipe) 91 communicating with the waste liquid tank 9 is connected to the outlet 75.

  Thereby, the waste liquid overflowing from the overflow outlet 76 is guided to the waste liquid tank 9 through the pipe 92, the first opening 771, the through hole 77, the second opening 772, and the pipe 93. Similarly to the overflow outlet 76, the first opening 771 is provided on the inflow ports 72, 73, 74 side with respect to the buffer space 71, so that a pipe 92 connecting the overflow outlet 76 and the first opening 771 is provided. It is possible to prevent a complicated flow path from being formed and to prevent the pipe 92 from being clogged.

  Further, since the second opening 772 is provided on the outlet 75 side with respect to the buffer space 71, the pipe 93 that connects the second opening 772 to the waste liquid tank 9 and the outlet 75 are connected to the waste liquid tank 9. The pipes 91 can be arranged close to each other and connected to the waste liquid tank 9 through a short flow path. In this example, the pipes 91 and 93 are arranged close to each other vertically.

  Thereby, it is possible to prevent the pipes 91 and 93 from forming a complicated flow path, to prevent the pipes 91 and 93 from being clogged, and to easily perform maintenance on the pipes 91 and 93. . Each of the pipes 91 and 93 may be provided with a label or the like so that an analyst can easily understand that one pipe 91 communicates with the outlet 75 and the other pipe 93 communicates with the overflow outlet 76. preferable.

  In the present embodiment, the pipes 91 and 93 communicating with the waste liquid tank 9 are formed of a transparent material. Thereby, the inside of the transparent piping 91 and 93 can be visually confirmed and it can be easily confirmed whether it is clogged. In particular, since the pipes 91 and 93 are arranged close to each other, if these pipes 91 and 93 are transparent, it can be easily confirmed at a time whether or not these pipes 91 and 93 are clogged. However, the pipes 91 and 93 are not limited to a transparent configuration, and may be translucent, for example. Moreover, not only the piping 91 and 93 but at least one of the piping 91, 92, and 93 may be transparent or translucent.

  As shown in FIG. 11, wires 911 and 931 made of metal such as stainless steel are provided inside the pipes 91 and 93 communicating with the waste liquid tank 9. These wires 911 and 931 extend spirally from one end to the other end in each pipe 91 and 93, and generate a repulsive force against the force acting on each pipe 91 and 93 from the outside. Thereby, each wire 911,931 functions as a blockade prevention member which prevents that the flow path inside each pipe | tube 91,93 is block | closed.

  Therefore, it is possible to prevent the waste liquid from flowing back and leaking by blocking the flow paths in the pipes 91 and 93. In particular, the pipes 91 and 93 are often deformed because they are attached to and detached from the waste liquid tank 9 when the waste liquid in the waste liquid tank 9 is discarded, but if the wires 911 and 931 are provided in these pipes 91 and 93, respectively. The flow path can be effectively prevented from being blocked by deformation. However, the wire is not limited to the pipes 91 and 93, as long as at least one of the pipes 91, 92 and 93 is provided with a wire. Moreover, as a blockage prevention member, if it is a member which can prevent that a flow path is blocked | closed, not only a wire but other arbitrary members can be used.

  In this embodiment, the buffer part 7 is arrange | positioned so that the upper part may incline toward the outflow port 75 side. That is, the central axis L of the main body 70 of the buffer portion 7 is inclined so as to gradually approach the near side (the direction in which the waste liquid tank 9 is pulled out from the pretreatment device 1) as it goes upward. As shown in FIG. 11, an opening 79 is formed in the upper part of the main body 70 of the buffer unit 7 on the outlet 75 side (front side) with respect to the buffer space 71. As shown in FIG. 9, the buffer unit 7 is located above the waste liquid tank 9, that is, above the rack 90.

  Thus, by arranging the buffer portion 7 to be inclined, even if both the outlet port 75 and the overflow outlet 76 are clogged, the outlet portion 75 side (front side) from the opening 79 in the upper portion of the buffer portion 7. Side) can overflow the waste liquid. As a result, the waste liquid can be prevented from flowing back, and the waste liquid overflowing from the opening 79 can be dropped onto the rack 90 of the waste liquid tank 9 provided on the outflow port 75 side. Leaking out of the processing apparatus 1 can be effectively prevented.

  In the above embodiment, the configuration in which the three inlets 72, 73, and 74 are formed in the buffer unit 7 has been described. However, the present invention is not limited to such a configuration, and for example, an inflow port 74 that communicates with the dispensing port 32 or the cold insulation unit 81 may be omitted, or an inflow port that communicates with other portions may be formed. In addition, the cleaning port 45 for cleaning the sample dispensing probe 20 a and the cleaning port 46 for cleaning the reagent dispensing probe 26 a both communicate with the buffer space 71 in the buffer unit 7 through the inlets 72 and 73. Not only such a configuration, but one of the inflow ports 72 and 73 may be omitted.

  In the above embodiment, the case where the dispensing position of the sample or reagent discharged from the sample dispensing probe 20a or the reagent dispensing probe 26a is in the separation container 50 on the dispensing port 32 has been described. However, not only the separation container 50 as in the above embodiment, but also when the sample or reagent is dispensed to a separation container having another configuration, or the sample or reagent is dispensed to a dispensing position other than the separation container. Such a configuration may be adopted.

  Moreover, in the above embodiment, the structure which isolate | separates the sample in the separation container 50 by making the inside of the installation space 30a of the filtration port 30 into a negative pressure was demonstrated. However, the configuration is not limited to such a configuration, and the configuration may be such that the sample in the separation container 50 is separated by pressurizing the inside of the separation container 50.

DESCRIPTION OF SYMBOLS 1 Pretreatment apparatus 1a Operation display part 2 Sample installation part 4 Sample rack 6 Sample container 7 Buffer part 8 Reagent installation part 9 Waste liquid tank 10 Reagent container 12 Container holding part 14 Rotation part 16 Container rack 20 Sample dispensing arm 20a Sample dispensing Probe 22 Vertical axis 24 Transport arm 25 Holding part 26 Reagent dispensing arm 26a Reagent dispensing probe 29 Vertical axis 30 Filtration port 30a Installation space 31 Holding member 32 Dispensing port 34 Disposal port 36 Agitation part 36a Agitation port 38, 40 Temperature control Port 42 Sample transfer part 43 Transfer port 44 Moving part 45, 46 Washing port 50 Separation container 51 Skirt part 52 Separation layer 54 Recovery container 55 Negative pressure load mechanism 70 Main body 71 Buffer space 72, 73, 74 Inlet 75 Outlet 76 Overflow Outlet 77 Through hole 78 Lid 79 Open Part 81 cold portion 90 rack 91, 92, 93 pipe 94 merging pipe 95 caps 96, 97, 98 merging pipe 100 LC
101 Autosampler 200 MS
201 Ionization section 202 Mass spectrometry section 321 Piping 451, 461, 811 Inflow pipe 452, 462, 812 Branch pipe 453, 463, 813 Piping 771, 772 Opening 810 Junction pipe 811 Inflow pipe 911, 931 Wire

Claims (10)

A probe for aspirating and dispensing a sample or reagent;
A cleaning section for cleaning the probe with a cleaning liquid;
A buffer unit into which waste liquid from the cleaning unit flows,
A waste liquid tank in which the waste liquid guided through the buffer unit is stored,
In the buffer part,
An inlet into which the waste liquid from the washing section flows,
An outlet through which the waste liquid flows into the waste liquid tank;
A buffer space for guiding the waste liquid flowing in from the inlet to the outlet;
A pre-processing apparatus, wherein an overflow outlet is provided at a position higher than the outlet in the buffer space and guides waste liquid overflowing from the buffer space to the waste liquid tank. The inlet and the outlet are formed on opposite sides of the buffer space,
The pre-processing apparatus according to claim 1, wherein the overflow outlet is provided on the inlet side with respect to the buffer space. The buffer section includes a first opening provided on the inlet side with respect to the buffer space, a second opening provided on the outlet side with respect to the buffer space, the first opening, and the A through hole communicating with the second opening is formed,
A first pipe connecting the overflow outlet to the first opening;
A second pipe connecting the second opening to the waste liquid tank;
The pretreatment device according to claim 2, further comprising a third pipe that connects the outlet to the waste liquid tank.   The pretreatment device according to claim 3, further comprising a junction pipe that joins the second pipe and the third pipe to connect to the waste liquid tank.   5. The pretreatment device according to claim 3, wherein at least one of the first pipe, the second pipe, and the third pipe is transparent or translucent.   The at least one of said 1st piping, said 2nd piping, and said 3rd piping is provided with the obstruction | occlusion prevention member which prevents that the internal flow path is obstruct | occluded. The pretreatment device according to any one of 5.   The pre-processing apparatus according to any one of claims 2 to 6, wherein the buffer section is disposed so that an upper portion thereof is inclined toward the outlet side. An inflow pipe connecting the cleaning part to the inflow port;
The pretreatment device according to claim 3, further comprising a branch pipe branched from the inflow pipe and communicating with the first pipe. The probe includes a sample dispensing probe for aspirating and dispensing a sample, and a reagent dispensing probe for aspirating and dispensing a reagent,
The inflow port includes a first inflow port through which waste liquid from the cleaning of the sample dispensing probe flows and a second inflow port through which waste liquid from the cleaning of the reagent dispensing probe flows. Item 9. The pretreatment device according to any one of Items 1 to 8.   The first inflow port is higher than the second inflow port, and is provided at a position where waste liquid flowing into the buffer space from the first inflow port does not come into contact with the second inflow port. The pretreatment device according to claim 9.

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