CN207516132U

CN207516132U - A kind of multi-functional Tip SPE of high throughput ultramicron

Info

Publication number: CN207516132U
Application number: CN201721241610.6U
Authority: CN
Inventors: 车雷
Original assignee: Shenzhen Step Life Science Co Ltd
Current assignee: Shenzhen Step Life Science Co Ltd
Priority date: 2017-09-26
Filing date: 2017-09-26
Publication date: 2018-06-19
Anticipated expiration: 2027-09-26

Abstract

The utility model discloses a kind of multi-functional Tip SPE of high-throughput ultramicron, and including functional stuffing and pipettor dry practice head, functional stuffing is made of polymer substrate, silica matrix or other matrix, and functional stuffing is fixed on the top of pipettor dry practice head；The utility model is simple in structure, realize high throughput, mass handles the process of biological sample, the biological sample amount down to 0.5 μ l can be handled, biological sample pre-treatment efficiency is the several times of current tradition SPE columns, greatly improve working efficiency, reduce the production cost of product itself, resource is saved, it can be used for doing the extraction and purification of large biological molecule, it can also be used to do the separation of solid and liquid of biological sample, it can be used to complete the extraction of entire biological sample, separation, purifying and concentration, while economizing on resources, reduce the reagent consumption of biological sample pre-treatment, discharging of waste liquid is minimized, protect environment.

Description

A kind of multi-functional Tip SPE of high throughput ultramicron

Technical field

The utility model is related to solid-phase extraction column class consumptive material field, the multi-functional Tip of specifically a kind of high throughput ultramicron SPE。

Background technology

At present, biological medicine, chemical analysis, food agricultural and veterinary chemicals retention analysis industry are used for doing biological sample pre-treatment（It crosses Filter/extraction/desalination/purifying/concentrating）Method be mostly traditional SPE columns, be divided into 1/3/6/12/25/50/300ml, although, The sample size that they are handled is bigger but cumbersome, mass, scale and miniaturization operation can not be realized, for micro- The processing of amount, ultramicron and trace sample, traditional SPE columns are difficult to realize.

The shortcomings that existing traditional SPE columns, specifically it is also manifested by the following aspects：1）Processing sample flux finite, one Secondary experiment, most multipotency handle 96 biological samples simultaneously；2）It is limited to handle sample size, all in more than 1ml, can not meet micro- The pre-treatment of amount, ultramicron and trace sample；3）Production is of high cost, since itself small product size is just big, produces it Required raw material and filler matrix are also more, and the production cost for leading to product itself is also just high；4）The degree of automation is not Height, moreover, generally requiring the coordinative composition of equipments such as super-magnum centrifuge, vacuum pump, negative pressure leaching device, inefficient, operation is extremely not Just, automation, mass processing biological sample can not be realized well；5）Function is single, can not meet it is multi-functional, diversified and Multiduty demand；6）Waste of resource pollutes environment, and since the sample size of traditional SPE columns processing is all in more than 1ml, some is very To 300ml has been reached, the reagent dosage of mating biological sample pre-treatment required in this way is also very big, not only increases reagent and disappears Consumption, increases processing cost, and reagent treatment used is that volatile corrosivity is harmful to human body and environmental toxic mostly Liquid, dosage is more, and discharging of waste liquid is more, also bigger to human body and environmental hazard.

However, with biological medicine, chemical analysis, the retention analysis of food agricultural and veterinary chemicals and the fast development of detection industry, need Magnanimity biochemical composition in a large amount of biological sample is analyzed and processed, it is therefore, mating to biological sample pre-treatment Instrument and equipment and reagent consumptive material propose higher requirement, such as high throughput, ultramicron, low cost, super convenient, mass, oneself Dynamicization, multi-functional, economic and environment-friendly etc., Tip SPE are exactly what is come into being under such a background.

Utility model content

The purpose of this utility model is to provide a kind of multi-functional Tip SPE of high-throughput ultramicron, to solve the above-mentioned back of the body The problem of being proposed in scape technology.

To achieve the above object, the utility model provides following technical solution：

A kind of multi-functional Tip SPE, the multi-functional Tip SPE of a kind high throughput ultramicron of high throughput ultramicron, It is characterized in that, including functional stuffing and pipettor dry practice head, functional stuffing is made of polymer substrate and silica matrix, liquid relief Device dry practice head includes 10ul, 100ul, 200ul, 500ul, 1ml, 5ml pipettor specification pipette tips, and functional stuffing is fixed on liquid relief The top of device dry practice head to realize the extraction and purification of large biological molecule, can also be used to do the separation of solid and liquid of biological sample, can For completing the extracting and developing of entire biological sample, purifying and concentration.

As further program of the utility model：The mode that functional stuffing is fixed on the top of pipettor dry practice head has three Kind：Film is filled to add filled sieve plate, sieve plate plus sieve plate and filler to be embedded in cone screen plate, and film and sieve plate are by high molecular weight Polymer forms.

As the utility model further scheme：Sieve plate is made of conductive active carbon filter core.

Compared with prior art, the beneficial effects of the utility model are：

1）High throughput at most can disposably handle 96-768 biological sample, realize high-throughput, mass simultaneously Handle the process of biological sample；

2）There is unique advantage for handling micro, ultramicron and trace biology sample, can handle down to 0.5 μ l Biological sample amount；

3）Efficiently, biological sample pre-treatment efficiency is the several times of current tradition SPE columns, greatly improves working efficiency, Realize the process of efficient mass processing biological sample, moreover it is possible to realize automatic business processing, automatic work station can be coordinated to realize complete Automatic business processing biological sample；

4）The Tip SPE of outstanding cost control ability, as low as 10 μ l, biological sample processing capacity itself is just than minimum Traditional SPE columns 1ml is 100 times small, and raw material used and filler matrix will be fewer than 1mlSPE columns very much, reduce product itself Production cost, saved resource；

5）Multi-functional, which is a multi-functional Tip SPE, can be used for doing the extraction of biological micromolecule with Purifying can also be used to do the separation of solid and liquid of biological sample, can be used to complete the extracting and developing of entire biological sample, purifying with Concentration, and be not limited to extract, filter, purify, concentrating, Solid Phase Extraction etc., at the same time it can also coordinate Automatic Workstation It completes the full-automatic work flow of entire biological sample, greatly facilitates vast researcher；

6）Automation, can coordinate Automatic Workstation to complete the extracting and developing of entire biological sample, purifying and concentration And etc..Whole process is without manual intervention；

7）Environmental protection while economizing on resources, reduces the reagent consumption of biological sample pre-treatment, discharging of waste liquid is down to It is minimum, protect environment.

Description of the drawings

Fig. 1 is a kind of structure diagram of the multi-functional Tip SPE of high-throughput ultramicron.

In figure：1- functional stuffings, 2- pipettor dry practice heads.

Specific embodiment

The technical solution of this patent is described in more detail With reference to embodiment.

Referring to Fig. 1, a kind of multi-functional Tip SPE of high throughput ultramicron, including functional stuffing 1 and pipettor dry practice head 2, functional stuffing 1 is made of polymer substrate and silica matrix, pipettor dry practice head 2 include 10ul, 100ul, 200ul, 500ul, 1ml, 5ml pipettor specification pipette tips, functional stuffing 1 are fixed on the top of pipettor dry practice head 2, can be used for doing life The extraction and purification of object macromolecular can also be used to do the separation of solid and liquid of biological sample, can be used to complete entire biological sample Extracting and developing, purifying and concentration.

Further：Functional stuffing 1 is fixed on there are three types of the modes on the top of pipettor dry practice head 2：Film is filled plus sieve plate, Sieve plate is filled to add sieve plate and filler to be embedded in cone screen plate, and film and sieve plate are made of the polymer of high molecular weight.

Further：Sieve plate is made of conductive active carbon filter core.

Utility model works principle：

1st, 1ml C18 Oligo desalination Tip SPE：By film plus the technology path of PE sieve plates, by C18 powder be filled in film and Between PE sieve plates, it is placed in 1ml pipette tips tip and forms.Such Tip SPE takes absorption purpose product, removes impurity, elutes mesh Product mode of operation come carry out oligonucleotide acid（Oligo）Desalting and purifying.

Concrete operation step is as follows：

1. the selection of technology path：The C18 powder particles of desalting and purifying Oligo are larger, are easy to the skill for adding PE sieve plates using film Art route；

2. the selection of film and PE sieve plates：In view of C18 powder used in desalting and purifying Oligo is 120 angstroms, 75um apertures, therefore The aperture for the film that we select is 35-40um.

3. according to demand of the client to desalting and purifying target product amount（4-6OD）And the test and appraisal to filling adsorption ability, Obtain filler amount of fill（20mg）And the specification of required pipette tips（1ml）With filler filling height as a result, so as to which selected PE is sieved The specification of plate：Aperture is 50um, a diameter of 3mm, thickness 1.6mm.

4. because needing to contact the organic reagents such as soda acid during desalting and purifying, therefore film and sieve plate have selected acid and alkali-resistance material Matter；

6. the selection of mode of operation：It is miscellaneous without adsorbing salt etc. because C18 powder has Oligo the suction-operated of specificity Matter so such Tip SPE takes absorption purpose product, removes impurity, elutes the mode of operation of purpose product to carry out；

7. make 1ml C18 desalination Tip SPE lab scale samples：20mg C18 powder is filled in the 1ml rifles with tip film In head, clear up pipette tips inner wall, select the PE sieve plates of specification beyond the Great Wall to suitable position, elastic appropriateness, both can, carry out batch life successively Production.

The product is 1ml C18 Oligo desalination Tip SPE.

2nd, 10ul silica flours PCR product purifying Tip SPE：The technology path of PE sieve plates is inlayed by taper, using inlaying Nanoscale silica flour is mounted to inside cone screen plate and goes by technique, forms the entirety that nano silica fume adds PE sieve plates, sieve plate taper Taper perfection with pipette tips inner cavity agrees with.Such Tip SPE takes absorption purpose product, removes impurity, elution purpose production The mode of operation of object carries out PCR product purifications.

The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party Formula, can also be under the premise of this patent objective not be departed from the knowledge that one skilled in the relevant art has It makes a variety of changes.

Claims

1. a kind of multi-functional Tip SPE of high throughput ultramicron, which is characterized in that including functional stuffing（1）With pipettor dry practice Head（2）, functional stuffing（1）It is to be made of polymer substrate or silica matrix, pipettor dry practice head（2）Including 10ul, 100ul, 200ul, 500ul, 1ml, 5ml pipettor specification pipette tips, functional stuffing（1）It is fixed on pipettor dry practice head（2）Top, can To be used for doing the extraction and purification of large biological molecule, it can also be used to do the separation of solid and liquid of biological sample, can be used to complete entire Extracting and developing, purifying and the concentration of biological sample.

2. the multi-functional Tip SPE of high throughput ultramicron according to claim 1, which is characterized in that functional stuffing（1）Gu Due to pipettor dry practice head（2）Top mode there are three types of：Film is filled to add filled sieve plate, sieve plate plus sieve plate and filler edge In cone screen plate, film and sieve plate are made of the polymer of high molecular weight.

3. the multi-functional Tip SPE of high throughput ultramicron according to claim 1, which is characterized in that sieve plate can be by conduction Active carbon filter core form.

4. the multi-functional Tip SPE of high throughput ultramicron according to claim 1, which is characterized in that functional stuffing weight Between 0.01ug-2g.

5. the multi-functional Tip SPE of high throughput ultramicron according to claim 1, which is characterized in that the biological sample of processing This amount volume is between 0.5 μ l-5ml.

6. the multi-functional Tip SPE of high throughput ultramicron according to claim 1, which is characterized in that coordinate full-automatic work Make station to complete the extracting and developing of entire biological sample, purifying and concentration overall process, realize full-automatic.