CN203728824U - Multi-cascade piping - Google Patents

Multi-cascade piping Download PDF

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Publication number
CN203728824U
CN203728824U CN201320859195.6U CN201320859195U CN203728824U CN 203728824 U CN203728824 U CN 203728824U CN 201320859195 U CN201320859195 U CN 201320859195U CN 203728824 U CN203728824 U CN 203728824U
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CN
China
Prior art keywords
liquid
holding pipe
pipe
holding
magnetic bead
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Expired - Lifetime
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CN201320859195.6U
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Chinese (zh)
Inventor
占伟
栾国彦
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XIAMEN ZEESAN BIOTECH CO Ltd
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XIAMEN ZEESAN BIOTECH CO Ltd
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Abstract

The utility model discloses a multi-cascade piping at least consisting of two connected liquid holding pipes through connecting. The multi-cascade piping is characterized in that the liquid holding pipes comprise at least one first liquid holding pipe and at least one second liquid holding pipe, wherein the volume of the first liquid holding pipe is 5-10mL, and the volume of the second liquid holding pipe is 3-5mL; the first liquid holding pipe is consistent with the second liquid holding pipe on the width direction of the multi-cascade piping, and the depth of the first liquid holding pipe is smaller than or equal to that of the second liquid holding pipe. When a sample needs to be treated by virtue of first liquid (held in the first liquid holding pipe) with a large volume and second liquid (held in the second liquid holding pipe) with a small volume respectively, the uniform mixing of the first liquid with the large volume, the sample and a magnetic bead can be ensured, the dosage of the sample can be effectively increased, and the waste of the first liquid can be avoided.

Description

A kind of multi-connected tubular strip
Technical field
The utility model relates to a kind of fluid container of biomedical sector, is specifically related to a kind of multi-connected tubular strip.
Background technology
Current commercial nucleic acid extraction is mainly to adopt centrifugal column method and paramagnetic particle method.
Centrifugal column method is mainly by large volume cracking, then vibration mixes, and its sample lysate adds centrifugal column, repeatedly centrifugal several times, realizes the enrichment of sample nucleic acid on centrifugal column.The method of centrifugal column is realizing aspect automatization extraction, and the bottleneck that need to overcome is larger, uses seldom at present in large automatic processing workstation.
Magnetic bead rule is to utilize magnetic bead absorption sample lysate amplifying nucleic acid, by pipettor repeatedly blow and beat, bar magnet mixes and coordinates liquor-transferring system to move liquid to realize effectively mixing of liquid mixture with rotation, the vibration of bar magnet cover and analogue with the moving up and down of bar magnet cover and analogue, bar magnet, thereby realize the functional steps such as effective combination, washing of effective cracking, sample nucleic acid and the magnetic bead particles of sample; But the volume for the liquid-holding pipe of the commercial multi-connected tubular strip of paramagnetic particle method nucleic acid extraction is consistent, and volume is no more than 5mL, and in the time that the amount of sample nucleic acid washings and the elutriant volume in liquid-holding pipe is suitable, the amount of sample lysate often approaches or exceedes the volume of liquid-holding pipe, causes the even waste of sample of difficulty that in working process, lysate and magnetic bead mix.
Utility model content
The purpose of this utility model is to overcome prior art defect, and a kind of multi-connected tubular strip is provided.
The technical solution of the utility model is as follows:
A kind of multi-connected tubular strip, at least be formed by connecting by two connected liquid-holding pipes, described liquid-holding pipe comprises at least one the first liquid-holding pipe and at least one the second liquid-holding pipe, the volume of the first liquid-holding pipe is 5~10mL, the volume of the second liquid-holding pipe is 3~5mL, the first liquid-holding pipe is consistent on the width of multi-connected tubular strip with the second liquid-holding pipe, and the degree of depth of the first liquid-holding pipe is less than or equal to the degree of depth of the second liquid-holding pipe.
In a preferred embodiment of the present utility model, also comprise at least one the 3rd liquid-holding pipe, the volume of the 3rd liquid-holding pipe is 2~3mL, consistent on the width of multi-connected tubular strip with the second liquid-holding pipe.
In a preferred embodiment of the present utility model, the mouth of pipe of each liquid-holding pipe forms the shirt rim that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.
In a preferred embodiment of the present utility model, every two adjacent liquid-holding pipes couple together by the shirt rim of the mouth of pipe, and the shirt rim of each liquid-holding pipe mouth of pipe is linked to be the structure of integral planar.
In a preferred embodiment of the present utility model, the bottom of described the first liquid-holding pipe and the second liquid-holding pipe is equipped with the screens for locating.
Another technical scheme of the present utility model is as follows:
Apply the method that above-mentioned multi-connected tubular strip carries out magnetic bead solution and mix transfer, in described the first liquid-holding pipe, first liquid is housed, in first liquid, include magnetic bead; In described the second liquid-holding pipe, second liquid is housed, specifically comprises the steps:
(1) mixture of the first liquid in described the first liquid-holding pipe and magnetic bead is carried out to the operation that mixes of at least two points, magnetic bead is dispersed in first liquid, these at least two points not exclusively overlap in the vertical;
(2) bar magnet is placed in the dispersed first liquid that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(3) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from above-mentioned first liquid, then be placed in the second liquid of the second liquid-holding pipe;
(4) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described second liquid because of shifting out from bar magnet cover surface of bar magnet;
(5) second liquid in described the second liquid-holding pipe and the mixture of magnetic bead are mixed to operation, magnetic bead is dispersed in second liquid.
In a preferred embodiment of the present utility model, described step (1) is specially: bar magnet is placed in described first liquid, operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (1) is specially: bar magnet is placed in described first liquid, at least two points that operation bar magnet is enclosed within the first liquid-holding pipe move up and down, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (1) is specially: at least two points of operating liquid transfer device in the first liquid of the first liquid-holding pipe are blown and beaten, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (5) is specially: operation bar magnet cover moves up and down, and magnetic bead is dispersed in second liquid.
The beneficial effects of the utility model are:
1, liquid-holding pipe of the present utility model comprises at least one the first liquid-holding pipe and at least one the second liquid-holding pipe, and the volume of the first liquid-holding pipe is greater than the volume of the second liquid-holding pipe; In the time running into sample needs respectively through the first liquid (being contained in the first liquid-holding pipe) of large volume and the processing of the second liquid (being contained in the second liquid-holding pipe) of small volume, can ensure that large volume first liquid, sample mix with the even of magnetic bead, avoid the waste of first liquid;
2, the volume of the first liquid-holding pipe of the present utility model is 5~10mL, makes the operation of sample lysate more than disposable processing 5mL volume become possibility;
3, multi-connected tubular strip of the present utility model is under the cooperation of the first liquid-holding pipe and the second liquid-holding pipe, the magnetic bead that can realize large volume first liquid, the small volume second liquid of sample mixes jump operation and realizes on same multi-connected tubular strip, handiness is good, and compatible same set of bar magnet and the bar magnet cover that uses treatment unit;
4, because the volume of the first liquid-holding pipe is larger, the liquid level of the first liquid that makes original same volume in this first liquid-holding pipe reduces, and the minimizing that the consumption of the second liquid using afterwards can be suitable reduces extraction cost; Along with the reduction of liquid level, reduce the risk that reagent solution spills, reduced the aerocolloidal probability of generation, further reduce the risk of crossed contamination;
Brief description of the drawings
Fig. 1 is the perspective view of the multi-connected tubular strip of the utility model embodiment 1.
Fig. 2 is the perspective view of the multi-connected tubular strip of the utility model embodiment 3.
Embodiment
Below by embodiment in connection with accompanying drawing, the technical solution of the utility model is further detailed and is described.
Embodiment 1
As shown in Figure 1, a kind of multi-connected tubular strip 1, at least be formed by connecting by two connected liquid-holding pipes, described liquid-holding pipe comprises one first liquid-holding pipe 11 and multiple the second liquid-holding pipe 12, the volume of described the first liquid-holding pipe 11 is 5~10mL and is a strip, make the operation of sample lysate more than disposable processing 5mL volume become possibility, the volume of described the second liquid-holding pipe 12 be 3~5mL and for point the end cylindric, the bottom of above-mentioned the first liquid-holding pipe 11 and the second liquid-holding pipe 12 can be provided with respectively the screens 111 and 121 for locating.
Preferably, the first liquid-holding pipe 11 of described strip is consistent on the width of multi-connected tubular strip 1 with the second liquid-holding pipe 12, and the degree of depth of the first liquid-holding pipe 11 is less than or equal to the degree of depth of the second liquid-holding pipe 12,
Further preferred, the mouth of pipe of above-mentioned each liquid-holding pipe forms the shirt rim 14 that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.Every two adjacent liquid-holding pipes couple together by the shirt rim 14 of the mouth of pipe, and the structure of integral planar is practiced in the shirt rim 14 of each liquid-holding pipe mouth of pipe.
Embodiment 2
Multi-connected tubular strip 1 described in embodiment 1 carries out magnetic bead solution and mixes a method for transfer, in described the first liquid-holding pipe 11, first liquid is housed, and includes magnetic bead in first liquid; In described the second liquid-holding pipe 12, second liquid is housed, specifically comprises the steps:
(1) mixture of the first liquid in described the first liquid-holding pipe 11 and magnetic bead is carried out to the operation that mixes of at least two points, magnetic bead is dispersed in first liquid, these at least two points not exclusively overlap in the vertical;
(2) bar magnet is placed in the dispersed first liquid that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(3) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from above-mentioned first liquid, then be placed in the second liquid of the second liquid-holding pipe 12;
(4) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described second liquid because of shifting out from bar magnet cover surface of bar magnet;
(5) second liquid in described the second liquid-holding pipe 12 and the mixture of magnetic bead are mixed to operation, magnetic bead is dispersed in second liquid, concrete preferred mode is: operation bar magnet cover moves up and down, and magnetic bead is dispersed in second liquid.
Described step (1) is specially following three kinds of modes::
A is placed on bar magnet in described first liquid, and operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe 11, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
B is placed on bar magnet in described first liquid, and at least two points that operation bar magnet is enclosed within the first liquid-holding pipe 11 move up and down, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
At least two points of C operating liquid transfer device (band moves liquid hair washing) in the first liquid of the first liquid-holding pipe 11 are blown and beaten, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
The method of the present embodiment can make first liquid and the magnetic bead in the first liquid-holding pipe 11 fully mix, take into account the abundant mixing of second liquid and magnetic bead in the second liquid-holding pipe 12 simultaneously, its motion control hardware and software are all very simple only need be adjusted a little on the existing motor pattern of original magnetic bar type instrument for extracting nucleic acid, get final product practical function, and extensibility is good, can be applied to easily from small cores acid extraction unit to a series of different equipment such as large-scale workstation real large volume liquid sample processing requirements.
Embodiment 3
As shown in Figure 2, a kind of multi-connected tubular strip 1 comprises 12 and 1 the 3rd liquid-holding pipe 13 of 11,6 the second liquid-holding pipes of 1 the first liquid-holding pipe successively, wherein the volume of the first liquid-holding pipe 11 is 8mL and is strip, the volume of the second liquid-holding pipe 12 is 3mL and is the cylindric of the point end, the volume of the 3rd liquid-holding pipe 13 be 2mL and for point the end cylindric, the bottom of above-mentioned the first liquid-holding pipe 11, the second liquid-holding pipe 12 and the 3rd liquid-holding pipe 13 is respectively equipped with the screens 111,121 and 131 for locating.Described the first liquid-holding pipe 11, the second liquid-holding pipe 12 are consistent on the width of multi-connected tubular strip 1 with the 3rd liquid-holding pipe 13, and the degree of depth of the first liquid-holding pipe 11 is less than or equal to the degree of depth of the second liquid-holding pipe 12.The mouth of pipe of above-mentioned each liquid-holding pipe forms the shirt rim 14 that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.Every two adjacent liquid-holding pipes couple together by the shirt rim 14 of the mouth of pipe, and the structure of integral planar is practiced in the shirt rim 14 of each liquid-holding pipe mouth of pipe.
Embodiment 4
Multi-connected tubular strip 1 described in a kind of embodiment 4 carries out magnetic bead solution and mixes the method for transfer, in described the first liquid-holding pipe 11,4mL lysate (first liquid) is housed, 0.5mL magnetic bead suspension (second liquid), 1.2mL the first washings, 1.2mL the second washings, 1.5mL the 3rd washings, 1.5mL the 4th washings and 1.6mL the 5th washings are housed in 6 the second liquid-holding pipes 12 successively, in described the 3rd liquid-holding pipe 13,0.2mL elutriant is housed, specifically comprises the steps:
(1) in the first liquid-holding pipe 11, add 2mL sample, mix operation, concrete optimal way is: operation bar magnet cover moves up and down, and lysate is mixed with sample;
(2) bar magnet is placed in bar magnet cover, stretches in first second liquid-holding pipe 12, and the magnetic bead in the magnetic bead suspension of absorption 0.5mL wherein, is transferred to this magnetic bead in the liquid of the first liquid-holding pipe 11;
(3) mixture of the liquid in described the first liquid-holding pipe 11 and magnetic bead is carried out to the operation that mixes of at least two points, the time is 5min, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical;
(4) bar magnet is placed in the liquid of dispersed the first liquid-holding pipe 11 that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(5) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from the liquid of above-mentioned the first liquid-holding pipe 11, then be placed in the washings of second the second liquid-holding pipe 12;
(6) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described washings because of shifting out from bar magnet cover surface of bar magnet;
(7) mixture of the washings in described second the second liquid-holding pipe 12 and magnetic bead is mixed to operation 2min, magnetic bead is dispersed in washings, concrete preferred mode is: operation bar magnet cover moves up and down, and magnetic bead is dispersed in the washings of second the second liquid-holding pipe 12;
(8), by the identical operation of above-mentioned steps (2) to (7), the washing (time is followed successively by 2min, 1~2min, 1~2min and 10~60s) that mixes of the magnetic bead successively washings in the 3rd to the 6th the second liquid-holding pipe 12 is finally entered in the elutriant in the 3rd liquid-holding pipe 13 and mixes wash-out 5~10 minutes.
Described step (1) is specially following three kinds of modes::
A is placed on bar magnet in the liquid of described the first liquid-holding pipe 11, operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe 11, magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
B is placed on bar magnet in the liquid of described the first liquid-holding pipe 11, and at least two points that operation bar magnet is enclosed within the first liquid-holding pipe 11 move up and down, and magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
At least two points of C operating liquid transfer device (band moves liquid hair washing) in the liquid of the first liquid-holding pipe 11 are blown and beaten, and magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
The method of the present embodiment can make liquid and the magnetic bead in the first liquid-holding pipe 11 fully mix, take into account the abundant mixing of washings and magnetic bead in second the second liquid-holding pipe 12 simultaneously, its motion control hardware and software are all very simple only need be adjusted a little on the existing motor pattern of original magnetic bar type instrument for extracting nucleic acid, get final product practical function, and extensibility is good, can be applied to easily from small cores acid extraction unit to a series of different equipment such as large-scale workstation real large volume liquid sample processing requirements.
The above, it is only preferred embodiment of the present utility model, therefore can not limit according to this scope that the utility model is implemented, the equivalence of doing according to the utility model the scope of the claims and description changes and modifies, and all should still belong in the scope that the utility model contains.

Claims (5)

1. a multi-connected tubular strip, at least be formed by connecting by two connected liquid-holding pipes, it is characterized in that: described liquid-holding pipe comprises at least one the first liquid-holding pipe and at least one the second liquid-holding pipe, the volume of the first liquid-holding pipe is 5~10mL, the volume of the second liquid-holding pipe is 3~5mL, the first liquid-holding pipe is consistent on the width of multi-connected tubular strip with the second liquid-holding pipe, and the degree of depth of the first liquid-holding pipe is less than or equal to the degree of depth of the second liquid-holding pipe.
2. a kind of multi-connected tubular strip as claimed in claim 1, is characterized in that: also comprise at least one the 3rd liquid-holding pipe, the volume of the 3rd liquid-holding pipe is 2~3mL, consistent on the width of multi-connected tubular strip with the second liquid-holding pipe.
3. a kind of multi-connected tubular strip as claimed in claim 1, is characterized in that: the mouth of pipe of each liquid-holding pipe forms the shirt rim that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.
4. a kind of multi-connected tubular strip as claimed in claim 2, is characterized in that: every two adjacent liquid-holding pipes couple together by the shirt rim of the mouth of pipe, and the shirt rim of each liquid-holding pipe mouth of pipe is linked to be the structure of integral planar.
5. a kind of multi-connected tubular strip as described in arbitrary claim in claim 1 to 4, is characterized in that: the bottom of described the first liquid-holding pipe and the second liquid-holding pipe is equipped with the screens for locating.
CN201320859195.6U 2013-12-24 2013-12-24 Multi-cascade piping Expired - Lifetime CN203728824U (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107102139A (en) * 2017-06-09 2017-08-29 北京化工大学 Prenatal and postnatal care five indices detect micro fluidic device
CN109060783A (en) * 2018-10-25 2018-12-21 成都博奥新景医学科技有限公司 A kind of single part chemical luminous immune detection method and system based on bar magnet method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107102139A (en) * 2017-06-09 2017-08-29 北京化工大学 Prenatal and postnatal care five indices detect micro fluidic device
CN107102139B (en) * 2017-06-09 2018-10-23 北京化工大学 Prenatal and postnatal care five indices detect micro fluidic device
CN109060783A (en) * 2018-10-25 2018-12-21 成都博奥新景医学科技有限公司 A kind of single part chemical luminous immune detection method and system based on bar magnet method

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C14 Grant of patent or utility model
GR01 Patent grant
C56 Change in the name or address of the patentee

Owner name: XIAMEN ZHISHAN BIOTECHNOLOGY CO., LTD.

Free format text: FORMER NAME: XIAMEN ZEESAN BIOTECH CO., LTD.

CP01 Change in the name or title of a patent holder

Address after: 361100 Taiwan science and technology enterprise incubation center, No. 88 Xiang Xiang Road, torch hi tech (Xiangan) District, Fujian, Xiamen E401

Patentee after: XIAMEN ZEESAN BIOTECH Co.,Ltd.

Address before: 361100 Taiwan science and technology enterprise incubation center, No. 88 Xiang Xiang Road, torch hi tech (Xiangan) District, Fujian, Xiamen E401

Patentee before: Xiamen Zeesan Biotech Co.,Ltd.

CX01 Expiry of patent term
CX01 Expiry of patent term

Granted publication date: 20140723