Utility model content
The purpose of this utility model is to overcome prior art defect, and a kind of multi-connected tubular strip is provided.
The technical solution of the utility model is as follows:
A kind of multi-connected tubular strip, at least be formed by connecting by two connected liquid-holding pipes, described liquid-holding pipe comprises at least one the first liquid-holding pipe and at least one the second liquid-holding pipe, the volume of the first liquid-holding pipe is 5~10mL, the volume of the second liquid-holding pipe is 3~5mL, the first liquid-holding pipe is consistent on the width of multi-connected tubular strip with the second liquid-holding pipe, and the degree of depth of the first liquid-holding pipe is less than or equal to the degree of depth of the second liquid-holding pipe.
In a preferred embodiment of the present utility model, also comprise at least one the 3rd liquid-holding pipe, the volume of the 3rd liquid-holding pipe is 2~3mL, consistent on the width of multi-connected tubular strip with the second liquid-holding pipe.
In a preferred embodiment of the present utility model, the mouth of pipe of each liquid-holding pipe forms the shirt rim that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.
In a preferred embodiment of the present utility model, every two adjacent liquid-holding pipes couple together by the shirt rim of the mouth of pipe, and the shirt rim of each liquid-holding pipe mouth of pipe is linked to be the structure of integral planar.
In a preferred embodiment of the present utility model, the bottom of described the first liquid-holding pipe and the second liquid-holding pipe is equipped with the screens for locating.
Another technical scheme of the present utility model is as follows:
Apply the method that above-mentioned multi-connected tubular strip carries out magnetic bead solution and mix transfer, in described the first liquid-holding pipe, first liquid is housed, in first liquid, include magnetic bead; In described the second liquid-holding pipe, second liquid is housed, specifically comprises the steps:
(1) mixture of the first liquid in described the first liquid-holding pipe and magnetic bead is carried out to the operation that mixes of at least two points, magnetic bead is dispersed in first liquid, these at least two points not exclusively overlap in the vertical;
(2) bar magnet is placed in the dispersed first liquid that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(3) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from above-mentioned first liquid, then be placed in the second liquid of the second liquid-holding pipe;
(4) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described second liquid because of shifting out from bar magnet cover surface of bar magnet;
(5) second liquid in described the second liquid-holding pipe and the mixture of magnetic bead are mixed to operation, magnetic bead is dispersed in second liquid.
In a preferred embodiment of the present utility model, described step (1) is specially: bar magnet is placed in described first liquid, operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (1) is specially: bar magnet is placed in described first liquid, at least two points that operation bar magnet is enclosed within the first liquid-holding pipe move up and down, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (1) is specially: at least two points of operating liquid transfer device in the first liquid of the first liquid-holding pipe are blown and beaten, magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
In a preferred embodiment of the present utility model, described step (5) is specially: operation bar magnet cover moves up and down, and magnetic bead is dispersed in second liquid.
The beneficial effects of the utility model are:
1, liquid-holding pipe of the present utility model comprises at least one the first liquid-holding pipe and at least one the second liquid-holding pipe, and the volume of the first liquid-holding pipe is greater than the volume of the second liquid-holding pipe; In the time running into sample needs respectively through the first liquid (being contained in the first liquid-holding pipe) of large volume and the processing of the second liquid (being contained in the second liquid-holding pipe) of small volume, can ensure that large volume first liquid, sample mix with the even of magnetic bead, avoid the waste of first liquid;
2, the volume of the first liquid-holding pipe of the present utility model is 5~10mL, makes the operation of sample lysate more than disposable processing 5mL volume become possibility;
3, multi-connected tubular strip of the present utility model is under the cooperation of the first liquid-holding pipe and the second liquid-holding pipe, the magnetic bead that can realize large volume first liquid, the small volume second liquid of sample mixes jump operation and realizes on same multi-connected tubular strip, handiness is good, and compatible same set of bar magnet and the bar magnet cover that uses treatment unit;
4, because the volume of the first liquid-holding pipe is larger, the liquid level of the first liquid that makes original same volume in this first liquid-holding pipe reduces, and the minimizing that the consumption of the second liquid using afterwards can be suitable reduces extraction cost; Along with the reduction of liquid level, reduce the risk that reagent solution spills, reduced the aerocolloidal probability of generation, further reduce the risk of crossed contamination;
Embodiment
Below by embodiment in connection with accompanying drawing, the technical solution of the utility model is further detailed and is described.
Embodiment 1
As shown in Figure 1, a kind of multi-connected tubular strip 1, at least be formed by connecting by two connected liquid-holding pipes, described liquid-holding pipe comprises one first liquid-holding pipe 11 and multiple the second liquid-holding pipe 12, the volume of described the first liquid-holding pipe 11 is 5~10mL and is a strip, make the operation of sample lysate more than disposable processing 5mL volume become possibility, the volume of described the second liquid-holding pipe 12 be 3~5mL and for point the end cylindric, the bottom of above-mentioned the first liquid-holding pipe 11 and the second liquid-holding pipe 12 can be provided with respectively the screens 111 and 121 for locating.
Preferably, the first liquid-holding pipe 11 of described strip is consistent on the width of multi-connected tubular strip 1 with the second liquid-holding pipe 12, and the degree of depth of the first liquid-holding pipe 11 is less than or equal to the degree of depth of the second liquid-holding pipe 12,
Further preferred, the mouth of pipe of above-mentioned each liquid-holding pipe forms the shirt rim 14 that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.Every two adjacent liquid-holding pipes couple together by the shirt rim 14 of the mouth of pipe, and the structure of integral planar is practiced in the shirt rim 14 of each liquid-holding pipe mouth of pipe.
Embodiment 2
Multi-connected tubular strip 1 described in embodiment 1 carries out magnetic bead solution and mixes a method for transfer, in described the first liquid-holding pipe 11, first liquid is housed, and includes magnetic bead in first liquid; In described the second liquid-holding pipe 12, second liquid is housed, specifically comprises the steps:
(1) mixture of the first liquid in described the first liquid-holding pipe 11 and magnetic bead is carried out to the operation that mixes of at least two points, magnetic bead is dispersed in first liquid, these at least two points not exclusively overlap in the vertical;
(2) bar magnet is placed in the dispersed first liquid that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(3) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from above-mentioned first liquid, then be placed in the second liquid of the second liquid-holding pipe 12;
(4) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described second liquid because of shifting out from bar magnet cover surface of bar magnet;
(5) second liquid in described the second liquid-holding pipe 12 and the mixture of magnetic bead are mixed to operation, magnetic bead is dispersed in second liquid, concrete preferred mode is: operation bar magnet cover moves up and down, and magnetic bead is dispersed in second liquid.
Described step (1) is specially following three kinds of modes::
A is placed on bar magnet in described first liquid, and operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe 11, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
B is placed on bar magnet in described first liquid, and at least two points that operation bar magnet is enclosed within the first liquid-holding pipe 11 move up and down, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
At least two points of C operating liquid transfer device (band moves liquid hair washing) in the first liquid of the first liquid-holding pipe 11 are blown and beaten, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical.
The method of the present embodiment can make first liquid and the magnetic bead in the first liquid-holding pipe 11 fully mix, take into account the abundant mixing of second liquid and magnetic bead in the second liquid-holding pipe 12 simultaneously, its motion control hardware and software are all very simple only need be adjusted a little on the existing motor pattern of original magnetic bar type instrument for extracting nucleic acid, get final product practical function, and extensibility is good, can be applied to easily from small cores acid extraction unit to a series of different equipment such as large-scale workstation real large volume liquid sample processing requirements.
Embodiment 3
As shown in Figure 2, a kind of multi-connected tubular strip 1 comprises 12 and 1 the 3rd liquid-holding pipe 13 of 11,6 the second liquid-holding pipes of 1 the first liquid-holding pipe successively, wherein the volume of the first liquid-holding pipe 11 is 8mL and is strip, the volume of the second liquid-holding pipe 12 is 3mL and is the cylindric of the point end, the volume of the 3rd liquid-holding pipe 13 be 2mL and for point the end cylindric, the bottom of above-mentioned the first liquid-holding pipe 11, the second liquid-holding pipe 12 and the 3rd liquid-holding pipe 13 is respectively equipped with the screens 111,121 and 131 for locating.Described the first liquid-holding pipe 11, the second liquid-holding pipe 12 are consistent on the width of multi-connected tubular strip 1 with the 3rd liquid-holding pipe 13, and the degree of depth of the first liquid-holding pipe 11 is less than or equal to the degree of depth of the second liquid-holding pipe 12.The mouth of pipe of above-mentioned each liquid-holding pipe forms the shirt rim 14 that can bond with sealed membrane, and sealed membrane is bonded on group limit, makes each liquid-holding pipe independent sealed.Every two adjacent liquid-holding pipes couple together by the shirt rim 14 of the mouth of pipe, and the structure of integral planar is practiced in the shirt rim 14 of each liquid-holding pipe mouth of pipe.
Embodiment 4
Multi-connected tubular strip 1 described in a kind of embodiment 4 carries out magnetic bead solution and mixes the method for transfer, in described the first liquid-holding pipe 11,4mL lysate (first liquid) is housed, 0.5mL magnetic bead suspension (second liquid), 1.2mL the first washings, 1.2mL the second washings, 1.5mL the 3rd washings, 1.5mL the 4th washings and 1.6mL the 5th washings are housed in 6 the second liquid-holding pipes 12 successively, in described the 3rd liquid-holding pipe 13,0.2mL elutriant is housed, specifically comprises the steps:
(1) in the first liquid-holding pipe 11, add 2mL sample, mix operation, concrete optimal way is: operation bar magnet cover moves up and down, and lysate is mixed with sample;
(2) bar magnet is placed in bar magnet cover, stretches in first second liquid-holding pipe 12, and the magnetic bead in the magnetic bead suspension of absorption 0.5mL wherein, is transferred to this magnetic bead in the liquid of the first liquid-holding pipe 11;
(3) mixture of the liquid in described the first liquid-holding pipe 11 and magnetic bead is carried out to the operation that mixes of at least two points, the time is 5min, and magnetic bead is dispersed in first liquid, and these at least two points not exclusively overlap in the vertical;
(4) bar magnet is placed in the liquid of dispersed the first liquid-holding pipe 11 that has magnetic bead by bar magnet, makes magnetic bead be adsorbed on the surface of bar magnet cover by the effect of bar magnet;
(5) bar magnet the bar magnet cover that have adsorbed magnetic bead are shifted out from the liquid of above-mentioned the first liquid-holding pipe 11, then be placed in the washings of second the second liquid-holding pipe 12;
(6) bar magnet is shifted out from bar magnet cover, be adsorbed on bar magnet and overlap surperficial magnetic bead and come off and enter described washings because of shifting out from bar magnet cover surface of bar magnet;
(7) mixture of the washings in described second the second liquid-holding pipe 12 and magnetic bead is mixed to operation 2min, magnetic bead is dispersed in washings, concrete preferred mode is: operation bar magnet cover moves up and down, and magnetic bead is dispersed in the washings of second the second liquid-holding pipe 12;
(8), by the identical operation of above-mentioned steps (2) to (7), the washing (time is followed successively by 2min, 1~2min, 1~2min and 10~60s) that mixes of the magnetic bead successively washings in the 3rd to the 6th the second liquid-holding pipe 12 is finally entered in the elutriant in the 3rd liquid-holding pipe 13 and mixes wash-out 5~10 minutes.
Described step (1) is specially following three kinds of modes::
A is placed on bar magnet in the liquid of described the first liquid-holding pipe 11, operation bar magnet is enclosed within continuous back and forth movement between at least two points in the same plane in the first liquid-holding pipe 11, magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
B is placed on bar magnet in the liquid of described the first liquid-holding pipe 11, and at least two points that operation bar magnet is enclosed within the first liquid-holding pipe 11 move up and down, and magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
At least two points of C operating liquid transfer device (band moves liquid hair washing) in the liquid of the first liquid-holding pipe 11 are blown and beaten, and magnetic bead is dispersed in this liquid, and these at least two points not exclusively overlap in the vertical.
The method of the present embodiment can make liquid and the magnetic bead in the first liquid-holding pipe 11 fully mix, take into account the abundant mixing of washings and magnetic bead in second the second liquid-holding pipe 12 simultaneously, its motion control hardware and software are all very simple only need be adjusted a little on the existing motor pattern of original magnetic bar type instrument for extracting nucleic acid, get final product practical function, and extensibility is good, can be applied to easily from small cores acid extraction unit to a series of different equipment such as large-scale workstation real large volume liquid sample processing requirements.
The above, it is only preferred embodiment of the present utility model, therefore can not limit according to this scope that the utility model is implemented, the equivalence of doing according to the utility model the scope of the claims and description changes and modifies, and all should still belong in the scope that the utility model contains.