CN211987210U - High-flux cross-contamination-prevention solid phase extraction device - Google Patents

High-flux cross-contamination-prevention solid phase extraction device Download PDF

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Publication number
CN211987210U
CN211987210U CN202020092129.0U CN202020092129U CN211987210U CN 211987210 U CN211987210 U CN 211987210U CN 202020092129 U CN202020092129 U CN 202020092129U CN 211987210 U CN211987210 U CN 211987210U
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universal joint
liquid guide
cover plate
phase extraction
hole
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CN202020092129.0U
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刘腾飞
董明辉
杨代凤
陆皓茜
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Suzhou Academy of Agricultural Sciences
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Suzhou Academy of Agricultural Sciences
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Abstract

The utility model discloses a high-flux cross-contamination prevention solid phase extraction device, which comprises a cover plate and a vacuum container, wherein a test tube rack is arranged in the vacuum container, and an air suction nozzle is arranged on the side wall; the side wall of the cover plate is provided with a pressure gauge and a pressure relief valve, the panel of the cover plate is provided with a plurality of through holes, each through hole is internally provided with a detachable universal joint, the center of the universal joint is provided with a liquid guide needle head penetrating hole, the needle head end of the liquid guide needle head penetrates out of the bottom of the universal joint, the joint end of the liquid guide needle head is connected with a flow control valve, and the flow control valve is connected with an SPE pillar; the liquid guide needle head is a standard industrial dispensing bayonet needle head. The device is provided with a plurality of sample extraction passages and independently detachable universal joints, and each universal joint is provided with a standard industrial needle head, so that the purchase cost of the liquid guide needle head special for the solid phase extraction device in the market is reduced; the detachable design mode of the universal joint and the liquid guide needle head really realizes the purpose of preventing cross contamination by solid phase extraction.

Description

High-flux cross-contamination-prevention solid phase extraction device
Technical Field
The utility model belongs to the technical field of processing apparatus before the sample, concretely relates to cross contamination type solid phase extraction device is prevented to high flux.
Background
Solid Phase Extraction (SPE) is a sample pretreatment technology developed in recent years, is developed by combining liquid-solid extraction and column liquid chromatography, is mainly used for separating, purifying and concentrating samples, can improve the recovery rate of analytes compared with the traditional liquid-liquid extraction method, more effectively separates the analytes from interfering components, reduces the sample pretreatment process, is simple to operate, saves time and labor, and has the characteristics of less organic solvent dosage, convenience, safety, high efficiency and the like. Solid phase extraction can be divided into four types according to the similar phase dissolution mechanism: reversed-phase SPE, normal-phase SPE, ion exchange SPE, and adsorption SPE.
Solid phase extraction is mostly used for treating liquid samples, extracting, concentrating and purifying semi-volatile and non-volatile compounds therein, and also for treating solid samples, which first have to be treated with a solvent, the components to be extracted being dissolved in the solvent and then extracted in the form of a solvent. At present, the solid phase extraction technology is widely applied to the fields of medicine, food, environment, commercial inspection, chemical industry and the like, and is an important sample pretreatment means in analysis and detection such as food safety, environmental monitoring, pharmaceutical analysis and the like.
The early solid phase extraction device that uses is simple and easy SPE post that is equipped with the filler, and SPE post comprises column tube, filler, push rod, realizes the flow of eluent in the column tube through manual promotion push rod, and this kind of mode is easy operation, but extraction efficiency is low, and time consumption is long, has increased experimenter's manipulation strength.
Along with the development of solid phase extraction technique, the SPE device is gradually made up, it comprises SPE pillar and auxiliary, the SPE pillar comprises the triplex, the column tube, sintering pad and filler, the SPE auxiliary generally has vacuum system, the vacuum pump, the device weathers, the inert gas source, large capacity sample thief and buffer bottle, the device can handle a plurality of different samples simultaneously, extraction efficiency is high, the intensity of labour of experimenter has been reduced, simultaneously every passageway has independent flow control valve, can adjust every independent passageway velocity of flow. However, the device has a certain defect that the liquid guide tube connected with the SPE small column on the cover plate is generally fixedly arranged on the bottom surface of the cover plate, when different extraction samples are replaced, the liquid guide tube part needs to be cleaned at the same time, the operation is complicated, the labor intensity is increased, and after the device is used for many times, the risk of cross contamination is inevitably caused. Although the prior art has developed a cross-contamination prevention type solid phase extraction apparatus, which prevents cross-contamination by replacing the liquid guide tube on the cover plate, the replaced liquid guide tube part is used as a matching accessory of the solid phase extraction apparatus, and must be purchased together with the apparatus, and the price of the special liquid guide tube purchased together is high, which greatly increases the extraction cost when a large amount of samples are processed. On the other hand, although the catheter is disposable, the sample remaining at the tip of the catheter may contaminate the catheter insertion hole during the replacement of the catheter, and when a new catheter is inserted, the contaminated catheter insertion hole may cause secondary contamination of the new catheter, failing to achieve true cross contamination prevention.
SUMMERY OF THE UTILITY MODEL
In order to solve the problems existing in the prior art, the utility model aims to provide a cross contamination type solid phase extraction device is prevented to high flux, and the device not only can realize the requirement that cross contamination is prevented to high flux, has greatly reduced the extraction cost simultaneously.
In order to achieve the above object, the present invention provides the following technical solutions:
a high-flux cross-contamination prevention type solid phase extraction device comprises a cover plate and a vacuum container, wherein a test tube rack is arranged in the vacuum container, and an air suction nozzle is arranged on the side wall of the vacuum container; the lateral wall of the cover plate is provided with a pressure gauge and a pressure relief valve, the panel of the cover plate is provided with a plurality of through holes, a detachable universal joint is arranged in each through hole, the center of the universal joint is provided with a liquid guide needle penetrating hole, the needle end of the liquid guide needle penetrates out of the bottom of the universal joint, the joint end of the liquid guide needle is connected with a flow control valve, and the flow control valve is connected with the SPE small column.
Further, the liquid guide needle head is a standard industrial dispensing bayonet needle head.
Furthermore, a sealing gasket is arranged on the bottom surface of the contact surface of the cover plate and the vacuum container, and a cover plate supporting leg is arranged on each corner of the bottom surface of the cover plate.
Furthermore, the through hole on the cover plate is sequentially provided with a first pore passage and a second pore passage from top to bottom, the inner diameter of the second pore passage is smaller than that of the first pore passage, and the joint surface of the first pore passage and the second pore passage forms a first limiting table; the first section external diameter of universal joint and the internal diameter phase-match of first pore, the second section external diameter of universal joint and the internal diameter phase-match of second pore, the universal joint carries out spacingly in the through-hole and through first spacing platform.
Further, the head of the first section of the universal joint is exposed out of the top surface of the cover plate.
Furthermore, the periphery of the top of the liquid guide needle penetrating hole is provided with a circle of groove to form a second limiting table, and the needle end of the liquid guide needle is limited on the universal joint through the second limiting table.
Further, the test-tube rack is placed the board by support bottom plate, a plurality of test tube and many altitude mixture control poles are constituteed, many altitude mixture control poles are fixed on the support bottom plate, and a plurality of test tubes are placed the board parallel and are worn to establish on the altitude mixture control pole.
Furthermore, a tray is placed at the bottom of the vacuum container, drain holes are formed in the tray, and a pair of hand-held grooves are formed in two opposite side walls of the tray.
Has the advantages that: the utility model discloses a high-flux cross contamination prevention type solid phase extraction device, which is provided with a plurality of sample extraction paths and an independently-disassembled universal joint, wherein the universal joint can be matched with standard industrial needles of different materials, different lengths and different thicknesses without special design and manufacture, thereby greatly reducing the purchase cost of a special liquid guide needle head of the solid phase extraction device in the market; meanwhile, the universal joint can be produced in large batch, and the universal joint can be used as a disposable part due to the detachable design mode, so that the novel universal joint and the liquid guide needle head can be used for extracting each sample, and the purpose of preventing cross contamination in solid phase extraction is really realized.
Drawings
FIG. 1 is a schematic structural diagram of the solid phase extraction apparatus of the present invention.
Fig. 2 is a schematic structural diagram of the cover plate of the present invention.
Fig. 3 is a partial cross-sectional view of the cover plate after the universal joint is assembled.
Fig. 4 is the structure schematic diagram of the test tube rack of the present invention.
Fig. 5 is a schematic structural view of the tray of the present invention.
In the figure: 1. a cover plate; 2. a vacuum vessel; 3. a test tube rack; 31. a bracket base plate; 32. a test tube placing plate; 33. a height adjustment lever; 4. a suction nozzle; 5. a pressure gauge; 6. a pressure relief valve; 7. a through hole; 71. a first duct; 72. a second duct; 8. a universal joint; 81. the liquid guide needle head penetrates into the hole; 82. a liquid-guiding needle head; 83. a first stage; 84. a second stage; 85. a second limit table; 9. A tray; 91. a drain hole; 92. carrying out grooving by hand; 10. a flow control valve; 11. the cover plate supports the feet.
Detailed Description
The present invention is further described below with reference to specific examples, which are only exemplary and do not limit the scope of the present invention in any way. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention, and that such changes and modifications are intended to be included within the scope of the invention.
A high flux prevents cross contamination type solid phase extraction device, as shown in figure 1, the device includes the cover plate 1 and the vacuum container 2, the material of the vacuum container 2 can choose the glass jar of thickening pressure resistance; a test tube rack 3 is arranged in the vacuum container, an air suction nozzle 4 is arranged on the side wall of the vacuum container 2, the air suction nozzle 4 is connected with a buffer bottle by a guide tube, the buffer bottle is connected with a vacuum pump by the guide tube, the guide tube can be a standard plastic steel guide tube or an all-plastic guide tube, and the buffer bottle can be a standard GL45 safety buffer bottle cap; be equipped with manometer 5 and relief valve 6 on the lateral wall of apron 1, be equipped with a plurality of through-holes 7 on the panel of apron 1, the number of sample has been decided to the number of through-hole, also can mark a three-way valve on every through-hole, increases the sample quantity on every through-hole.
A detachable universal joint 8 is arranged in each through hole 7, a liquid guide needle penetrating hole 81 is arranged in the center of the universal joint 8, the needle end of a liquid guide needle 82 penetrates out of the bottom of the universal joint 8, the joint end of the liquid guide needle is connected with a flow control valve 10, and the flow control valve 10 is connected with an SPE small column; the liquid guide needle 82 is a standard industrial dispensing bayonet needle, the length of the needle tube is 38mm-100mm, and the specification is as follows: the needle head can be purchased in the market, has wide sources and low price, can be made of all metal, plastic steel or all PP materials, has no length and thickness requirements (can be penetrated through a liquid guide needle head penetrating hole), does not need to be specially designed and manufactured, and thereby greatly reduces the purchase cost of the liquid guide needle head special for the solid phase extraction device in the market; meanwhile, the universal joint can be produced in large batch, and the universal joint can be used as a disposable part due to the detachable design mode, so that the novel universal joint and the liquid guide needle head can be used for extracting each sample, and the purpose of preventing cross contamination in solid phase extraction is really realized.
The bottom surface of the contact surface of the cover plate 1 and the vacuum container 2 is provided with a sealing gasket, and the sealing gasket is arranged to ensure that the device has good sealing property; each corner of the bottom surface of the cover plate is provided with a cover plate supporting leg 11, so that a user can conveniently place the cover plate on a workbench when removing the cover plate from the device.
As a matching design manner of the universal joint and the through hole, as shown in fig. 2 and 3, the through hole 7 on the cover plate 1 is sequentially provided with a first hole 71 and a second hole 72 from top to bottom, the inner diameter of the second hole 72 is smaller than that of the first hole 71, and the joint surface of the first hole 71 and the second hole 72 forms a first limit table; the outer diameter of the first section 83 of the universal joint 8 is matched with the inner diameter of the first pore channel 71, the outer diameter of the second section 84 of the universal joint 8 is matched with the inner diameter of the second pore channel 72, and the universal joint is clamped in the through hole and limited through the first limiting table. The universal joint is made of rubber materials and is assembled in the through hole in an interference fit mode, and therefore good sealing performance between the universal joint and the through hole is guaranteed.
In the design mode that the universal joint is matched with the through hole, the structure of the universal joint and the through hole can be designed into other alternative forms as long as the universal joint is detachably fixed in the through hole, and the universal joint can still keep good fixing performance and good sealing performance with the through hole in the vacuumizing process. Meanwhile, as a preferred embodiment, the universal connector can also be designed by referring to the structure of the existing luer connector, and then the through hole is designed into a matched structure.
Preferably, the head of the first section 83 of the universal joint 8 is exposed out of the top surface of the cover plate 1, and a user can replace and assemble the universal joint conveniently by only holding the head of the universal joint.
Preferably, as shown in fig. 3, a circle of grooves is formed on the top periphery of the liquid guide needle penetrating hole 81 to form a second limit table 85, and the needle end of the liquid guide needle is limited on the universal joint through the second limit table, so that the liquid guide needle can keep good fixation during the extraction process.
As shown in fig. 4, the test tube rack 3 is composed of a rack bottom plate 31, a plurality of test tube placing plates 32 and a plurality of height adjusting rods 33, the height adjusting rods 33 are fixed on the rack bottom plate 31, and the plurality of test tube placing plates 32 are parallelly arranged on the height adjusting rods 33 in a penetrating manner; wherein the test tube is placed board and can be held different volumetric test tubes or centrifuging tube.
As shown in fig. 5, a tray 9 is placed on the bottom of the vacuum container 2, a drain hole 91 is formed on the tray 9, and a pair of hand-carrying slots 92 are formed on two opposite side walls of the tray. The water on the outer wall of the vacuum container after being cleaned can be discharged through the water discharge hole 91, so that the problem that the vacuum container slides in the presence of water is solved; the portable slotted design facilitates movement of the device.
The device apron, apron supporting legs, test-tube rack, tray all select for use the plastics material of high anticorrosive rank to improve the device's performance.
The use method of the device is as follows:
1. placing the test tube rack into a vacuum container, and placing test tubes with corresponding specifications on the test tube rack;
2. connecting the conduit with an air exhaust nozzle of a vacuum container, and then sequentially connecting a buffer bottle and a vacuum pump;
3. covering the cover plate on a vacuum container, wherein each through hole corresponds to the test tube one by one;
4. assembling the universal joint in the through hole, inserting the liquid guide needle head, and assembling the flow control valve and the SPE small column;
5. closing the flow control valve and screwing the pressure release valve;
6. adding a sample to be extracted into the SPE small column;
7. opening the flow control valve and the vacuum pump, enabling the liquid to flow into the test tube through the SPE small column in a vacuum state, and controlling the flow rate of the liquid by adjusting the flow control valve in the extraction process;
8. after extraction is finished, the vacuum pump is closed, and the pressure in the vacuum container is released by unscrewing the pressure release valve;
9. and collecting the extracted sample, disassembling the universal joint and the liquid guide needle, cleaning or discarding the universal joint and the liquid guide needle, then replacing the universal joint and the liquid guide needle with new ones, and continuing to perform the next round of extraction operation.

Claims (8)

1. A high-flux cross-contamination prevention type solid phase extraction device is characterized by comprising a cover plate and a vacuum container, wherein a test tube rack is arranged in the vacuum container, and an air suction nozzle is arranged on the side wall of the vacuum container; the lateral wall of the cover plate is provided with a pressure gauge and a pressure relief valve, the panel of the cover plate is provided with a plurality of through holes, a detachable universal joint is arranged in each through hole, the center of the universal joint is provided with a liquid guide needle penetrating hole, the needle end of the liquid guide needle penetrates out of the bottom of the universal joint, the joint end of the liquid guide needle is connected with a flow control valve, and the flow control valve is connected with the SPE small column.
2. The high-throughput cross-contamination prevention type solid-phase extraction device of claim 1, wherein the liquid guide needle is a standard industrial dispensing bayonet needle.
3. The high-throughput cross-contamination prevention solid phase extraction device according to claim 1, wherein a sealing gasket is provided on the bottom surface of the contact surface of the cover plate and the vacuum vessel, and a cover plate supporting leg is provided on each corner of the bottom surface of the cover plate.
4. The high-throughput cross-contamination prevention solid phase extraction device according to claim 1, wherein the through hole of the cover plate is sequentially provided with a first hole channel and a second hole channel from top to bottom, the inner diameter of the second hole channel is smaller than that of the first hole channel, and the joint surface of the first hole channel and the second hole channel forms a first limit platform; the first section external diameter of universal joint and the internal diameter phase-match of first pore, the second section external diameter of universal joint and the internal diameter phase-match of second pore, the universal joint carries out spacingly in the through-hole and through first spacing platform.
5. The high throughput cross-contamination prevention solid phase extraction device of claim 4, wherein the first section of the universal joint is exposed at the top surface of the cover plate.
6. The high-throughput cross-contamination prevention type solid phase extraction device according to claim 1, wherein a circle of groove is formed on the periphery of the top of the liquid guide needle penetrating hole to form a second limiting table, and the needle end of the liquid guide needle is limited on the universal joint through the second limiting table.
7. The high-throughput cross-contamination prevention solid-phase extraction device according to claim 1, wherein the test tube rack comprises a rack bottom plate, a plurality of test tube placing plates and a plurality of height adjusting rods, the height adjusting rods are fixed on the rack bottom plate, and the plurality of test tube placing plates are parallelly arranged on the height adjusting rods in a penetrating manner.
8. The high-throughput cross-contamination prevention solid phase extraction device of claim 1, wherein a tray is placed at the bottom of the vacuum vessel, the tray is provided with a drain hole, and a pair of hand-held slots are formed on two opposite side walls of the tray.
CN202020092129.0U 2020-01-16 2020-01-16 High-flux cross-contamination-prevention solid phase extraction device Active CN211987210U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114018678A (en) * 2021-11-26 2022-02-08 中国科学院地理科学与资源研究所 Device and method for purifying and separating copper isotopes in soil

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114018678A (en) * 2021-11-26 2022-02-08 中国科学院地理科学与资源研究所 Device and method for purifying and separating copper isotopes in soil

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