CN111504754A - One-step sample purifying tube - Google Patents
One-step sample purifying tube Download PDFInfo
- Publication number
- CN111504754A CN111504754A CN201910094623.2A CN201910094623A CN111504754A CN 111504754 A CN111504754 A CN 111504754A CN 201910094623 A CN201910094623 A CN 201910094623A CN 111504754 A CN111504754 A CN 111504754A
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- China
- Prior art keywords
- plug
- needle cylinder
- needle
- purification
- push rod
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
Abstract
The invention relates to a one-step sample purifying tube, which comprises a threaded plug, a needle cylinder, sample pretreatment purifying fillers, a guide plate, a porous sieve plate and a push rod with an inert rubber plug, wherein the sample pretreatment purifying fillers are arranged on the threaded plug; a guide plate and a porous sieve plate are sequentially arranged at one end of the needle cylinder close to the needle head, and a sample pretreatment purification filler is arranged between the porous sieve plate and the push rod; the inner diameter of the needle cylinder is matched with the outer diameter of the inert rubber plug of the push rod; the diameters of the sieve plate and the flow guide plate are matched with the inner diameter of the needle cylinder, and the purification filler is sealed in the needle cylinder; the plug is in threaded connection with the needle cylinder, the inner opening of the plug is matched with the outlet of the needle cylinder, and the plug can completely seal the outlet of the needle head. The purification tube changes the conventional dispersed solid phase extraction mode by adopting a mode of prefilling a purification filler in a needle cylinder, and takes the previous operation steps (sample solution suction, sealing solution, vortex oscillation, adsorption purification, high-speed centrifugation, purification solution suction and filtration) as one step. After purification is finished, the plug is only required to be removed, the needle type filter is connected to the outlet of the needle cylinder, the push rod is pushed out of the solution, the solution is filtered, and the upper machine test solution can be obtained without centrifugation.
Description
Technical Field
The invention relates to sample pretreatment operation steps and a using device in an analytical detection process, and belongs to the field of sample preparation in analytical chemistry.
Background field of the invention
In the field of chemical analysis, sample pretreatment is always an important part in the process of detecting target compounds. The statistical result shows that the sample pretreatment in the sample pretreatment step occupies quite a lot of time, and some samples can even occupy 70% or more of the whole time; approximately half as much time as the detection analysis of the sample itself. The exploration and research of various new technologies and methods become one of the important subjects and development directions of modern analytical chemistry, and the rapid, simple and automatic pretreatment technology not only saves time and labor, but also can reduce errors caused by operation of different personnel and multiple transfers of samples, and simultaneously can avoid using a large amount of organic solvents and reduce pollution to the environment.
In the field of detection of pesticide and veterinary drug residues and mycotoxins, the mainstream sample pretreatment technology at present comprises solid phase extraction (SPE cartridge), gel permeation chromatography and a QuEChERS technology based on dispersed solid phase extraction. The SPE process needs multiple operations (balancing, sample loading, leaching, elution and the like), samples are easy to penetrate, the leaching speed needs to be controlled one by one, the operation is complex, and the repeatability is not high. And the gel permeation chromatography needs expensive equipment, the use amount of the organic solvent is large, and the popularization in a conventional laboratory is difficult to realize. The QuEChERS technology adopts a dispersed filler mode, has high recovery rate and good detection repeatability, but still needs multiple operation steps (transferring, whirling, centrifuging, film passing and the like) and has longer detection period. The detection of the edible agricultural products usually needs certain detection timeliness and needs to obtain results quickly, so that data support is provided for supervision, enterprise raw material purchase and quality evaluation. Therefore, the laboratory needs to develop a time-saving and labor-saving sample pretreatment technology for pesticide and veterinary drug residues and mycotoxins, and the requirements of rapid treatment and rapid screening are met. The fewer operation steps and the fewer appliances are used for sample pretreatment, the more accurate and reliable result is, the lower experiment cost is, and the higher efficiency is.
Disclosure of Invention
In order to solve the technical problems of multiple sample pretreatment steps and complex operation, the invention provides a one-step sample purification tube, which comprises a threaded plug, a needle cylinder, sample pretreatment purification filler, a guide plate, a porous sieve plate and a push rod with an inert rubber plug; a guide plate and a porous sieve plate are sequentially arranged at one end of the needle cylinder close to the needle head, and a sample pretreatment purification filler is arranged between the porous sieve plate and the push rod; the inner diameter of the needle cylinder is matched with the outer diameter of the inert rubber plug of the push rod; the diameters of the sieve plate and the flow guide plate are matched with the inner diameter of the needle cylinder, and the purification filler is sealed in the needle cylinder; the plug is in threaded connection with the needle cylinder, the inner opening of the plug is matched with the outlet of the needle cylinder, and the plug can completely seal the outlet of the needle head.
The syringe also comprises a needle head which is in interference fit with the outlet of the syringe.
The purification filler can be stored in the syringe under the sealing among the plug, the sieve plate and the inert rubber plug. The push rod moves back and forth in the syringe linearly, so that a certain volume of sample extraction solution can be sucked or liquid can be discharged as required. After the sample extraction solution is sucked, the solution can be sealed in the needle cylinder by the plug and the inert rubber plug, and the purification filler and the sample solution are fully mixed by a vortex oscillator or other modes to achieve the aim of purifying the solution. After purification is finished, the plug is only required to be removed, the needle head of the syringe is connected with the filter membrane, the push rod is pushed out of the solution, and the upper computer test solution can be obtained without centrifugation.
The inner diameter of the needle cylinder of the one-step sample purifying tube is matched with the outer diameter of the inert rubber plug of the push rod to form good sealing; the outer diameters of the sieve plate and the guide plate are matched with the inner diameter of the needle cylinder, so that the purification filler is sealed in the needle cylinder; the thread of the plug is matched with the thread of the needle cylinder, the inner opening of the plug is matched with the outlet of the needle cylinder, and the plug completely seals the outlet of the needle head; the needle head is matched with the outlet of the syringe.
The push rod with the inert rubber plug in the one-step sample purification tube can be pushed and pulled back and forth in the needle cylinder in a straight line, and can suck a certain volume of sample solution or discharge liquid according to needs.
The purification filler of the one-step sample purification tube is composed of various adsorption fillers according to different proportions, and mainly comprises anhydrous magnesium sulfate, C18 bonded silica gel, amino bonded silica gel, PSA, graphitized carbon black and the like.
The plug, the needle cylinder and the inert rubber plug of the push rod of the one-step sample purifying tube form excellent sealing, and the purifying filler is not prevented from being affected with damp and deteriorated. Meanwhile, the sample solution sucked by the needle cylinder can be strongly vortexed and oscillated by sealing the plug and the inert rubber plug, so that the adsorption and purification of the sample solution are enhanced.
The needle head, the needle cylinder, the push rod and the plug of the one-step sample purification tube can be made of PE, polytetrafluoroethylene or metal and other materials which do not adsorb or interfere with a target compound, the one-step sample purification tube can be processed into the needle cylinders with different volumes according to requirements, the typical shapes and the volumes are 3m L, 5m L, 10m L and the like, and the invention can be realized by only ensuring that the size of an inert rubber plug and the size of a sieve plate are matched with the inner diameter of the needle cylinder and the plug and the needle of the needle cylinder are matched.
Drawings
FIG. 1 is a schematic view of the entire one-step sample purification tube
FIG. 2 is a schematic view showing a state before the one-step type sample purification tube is used
FIG. 3 is a schematic diagram of a one-step sample purification tube for solution aspiration
1-a threaded plug; 1B-inner opening of the plug; 2-a needle head; 3-a needle cylinder; 3B-outlet of the syringe; 4-sample pretreatment and purification of filler; 5-porous sieve plate 6-guide plate; 7-a push rod; 7A-inert rubber plug; 7B-push rod force point;
Detailed Description
The one-step sample purification tube of the present invention will be described in detail with reference to the accompanying drawings 1-3 and specific examples.
FIG. 1 shows a schematic of the components of a one-step sample purification tube. The inner diameter of a needle cylinder of the one-step sample purifying tube is slightly smaller than the outer diameter of the inert rubber plug, and the inert rubber plug forms good sealing after being deformed; the outer diameters of the sieve plate and the guide plate are slightly larger than the inner diameter of the needle cylinder, and the purification filler is sealed in the needle cylinder through the deformation of the sieve plate and the guide plate; the thread of the plug is matched with the thread of the needle cylinder, the inner opening of the plug is matched with the outlet of the needle cylinder, and the plug completely seals the outlet of the needle head; the needle head is in threaded fit with the outlet of the needle cylinder, the sealing performance is excellent, and the sample extracting solution can be conveniently sucked.
The invention is operated in detail by following steps:
the sample is extracted by a certain chemical reagent, and the target compound is dissolved in the sample solution.
Taking off the plug (shown in figure 2), installing a needle (shown in figure 3), sucking the sample extracting solution, keeping the needle below the liquid level of the sample solution in the sucking process, and sucking a certain volume of the sample solution as required. And then, the needle head is upwards, the needle head is taken down, and the plug is installed.
And (3) uniformly mixing the whole needle cylinder with the sample solution and the purification filler on a vortex vibration instrument in a vortex manner for 30-60 s, or manually shaking to fully mix the filler and the extracting solution.
And (3) taking down the luer plug with the opening of the needle cylinder upward, installing a needle type filter, and respectively pushing out the purified liquid to carry out solvent replacement or directly carrying out machine test. The plug, the needle cylinder and the inert rubber plug of the push rod of the one-step sample purification tube form excellent sealing, and the purification filler is not prevented from being affected with damp and deteriorated. Meanwhile, the sample solution sucked by the needle cylinder can be subjected to strong vortex oscillation after being sealed by the plug and the inert rubber plug, so that the adsorption and purification of the sample solution are enhanced. The purifying filler may be any one or more of commercially available chemical adsorption fillers in various proportions, and commonly used fillers include anhydrous magnesium sulfate, C18 bonded silica gel, amino bonded silica gel, PSA, graphitized carbon black, neutral alumina, ion exchange resin, etc., which are not illustrated herein. The sealing mode of the invention ensures the long-term stability of the product, which is a special property that the prior related products do not have.
The material of the one-step sample purifying tube can be PE, polytetrafluoroethylene or metal, and other materials which do not adsorb or interfere with target compounds.
Claims (5)
1. The one-step sample purification tube comprises a threaded plug (1), a needle cylinder (3), a sample pretreatment purification filler (4), a porous sieve plate (5), a guide plate (6) and a push rod (7) with an inert rubber plug; the method is characterized in that: a guide plate and a porous sieve plate are sequentially arranged at one end of the needle cylinder close to the needle head, and a sample pretreatment purification filler is arranged between the porous sieve plate and the push rod; the inner diameter of the needle cylinder (3) is matched with the outer diameter of an inert rubber plug (7A) of the push rod (7); the diameters of the sieve plate (5) and the guide plate (6) are matched with the inner diameter of the needle cylinder (3) to seal the purifying filler (4) in the needle cylinder (3); the plug (1) is in threaded connection with the syringe (3), and the inner opening (1B) of the plug is matched with the outlet (3B) of the syringe, so that the plug can completely seal the outlet of the needle head.
2. The one-step sample purification tube of claim 1, further comprising a needle (2) that is an interference fit with the outlet (3B) of the barrel.
3. The one-step sample purge tube of claim 1, wherein a seal is formed between the stopper (1), the syringe (3) and the inert plug (7A) of the push rod (7).
4. The one-step sample purification tube of claim 2, wherein the needle, barrel, plunger and stopper are made of a material that does not adsorb or interfere with the target compound.
5. The one-step sample purification tube of claim 4, wherein the needle, barrel, plunger and stopper are made of PE, PTFE or metal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910094623.2A CN111504754A (en) | 2019-01-31 | 2019-01-31 | One-step sample purifying tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910094623.2A CN111504754A (en) | 2019-01-31 | 2019-01-31 | One-step sample purifying tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111504754A true CN111504754A (en) | 2020-08-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910094623.2A Pending CN111504754A (en) | 2019-01-31 | 2019-01-31 | One-step sample purifying tube |
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| CN (1) | CN111504754A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255343A (en) * | 2020-10-16 | 2021-01-22 | 宁波市疾病预防控制中心 | Method and kit for measuring fentanyl drugs in biological sample |
-
2019
- 2019-01-31 CN CN201910094623.2A patent/CN111504754A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112255343A (en) * | 2020-10-16 | 2021-01-22 | 宁波市疾病预防控制中心 | Method and kit for measuring fentanyl drugs in biological sample |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200807 |
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| WD01 | Invention patent application deemed withdrawn after publication |