CN110437980B - Nucleic acid extraction adsorption column - Google Patents
Nucleic acid extraction adsorption column Download PDFInfo
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- CN110437980B CN110437980B CN201910804185.4A CN201910804185A CN110437980B CN 110437980 B CN110437980 B CN 110437980B CN 201910804185 A CN201910804185 A CN 201910804185A CN 110437980 B CN110437980 B CN 110437980B
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- adsorption
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- adsorption column
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- nucleic acid
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- 238000001179 sorption measurement Methods 0.000 title claims abstract description 165
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 33
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 33
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 33
- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 49
- 239000000706 filtrate Substances 0.000 claims abstract description 44
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 230000000903 blocking effect Effects 0.000 claims abstract description 4
- 238000006073 displacement reaction Methods 0.000 claims description 7
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 5
- 210000003205 muscle Anatomy 0.000 description 5
- 238000012546 transfer Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000012356 Product development Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
Abstract
The invention particularly relates to a nucleic acid extraction adsorption column. The adsorption column comprises an adsorption column outer tube, a filtrate head, an adsorption column and a liquid moving column, wherein the filtrate head is positioned at the lower end of the adsorption column outer tube and is internally provided with an adsorption film; the lower end of the adsorption column is positioned in the outer tube of the adsorption column and can move up and down relative to the outer tube of the adsorption column, the adsorption column is also sleeved with a sealing sleeve, and the lower end of the adsorption column is provided with an adsorption hole through which nucleic acid passes; the pipetting column comprises a pipette with an opening at the upper end, a sealing head capable of blocking the adsorption hole at the lower end of the adsorption column is arranged at the lower end of the pipette, a plurality of pipetting holes are further formed in the pipette, and the maximum diameter of the sealing head is smaller than the inner diameter of the adsorption column. The nucleic acid extraction adsorption column has a simple structure, and is convenient for manual operation and mechanical automation operation.
Description
Technical Field
The invention relates to a nucleic acid separation and purification device, in particular to a nucleic acid extraction adsorption column.
Background
Nucleic acid extraction is a basic method of molecular biology, is the first step of all molecular biology experiments, is the starting point of downstream nucleic acid detection, diagnostic analysis or product development, and the quality and integrity of the extracted nucleic acid directly affect the research or diagnostic results, so nucleic acid extraction is one of the most critical operating steps of molecular biology. When the nucleic acid extraction is performed, the workload is great when the sample is more, and the manual operation efficiency is lower, so that how to build a set of mechanized nucleic acid extraction equipment is important.
Disclosure of Invention
In view of the above problems, an object of the present invention is to provide a nucleic acid extraction adsorption column which has a simple structure and is convenient for mechanical automation operation.
The technical scheme for solving the technical problems is as follows:
the invention provides a nucleic acid extraction adsorption column, which comprises an adsorption column outer tube, a filtrate head, an adsorption column and a liquid displacement column, wherein the filtrate head is arranged at the lower end of the adsorption column outer tube and is internally provided with an adsorption film; the lower end of the adsorption column is positioned in the outer tube of the adsorption column and can move up and down relative to the outer tube of the adsorption column, the adsorption column is also sleeved with a sealing sleeve, and the lower end of the adsorption column is provided with an adsorption hole through which nucleic acid passes; the pipetting column comprises a pipette with an opening at the upper end, a sealing head capable of blocking the adsorption hole at the lower end of the adsorption column is arranged at the lower end of the pipette, a plurality of pipetting holes are further formed in the pipette, and the maximum diameter of the sealing head is smaller than the inner diameter of the adsorption column.
Wherein, the adsorption column includes adsorption head, adsorption tube, absorption hole and sealing platform, and the adsorption head is located the lower extreme of adsorption tube and is conical structure, and the seal cover is established on the adsorption head, and the absorption hole is located on the adsorption head, and the bottom of adsorption tube below is provided with the sealing platform with the sealing head assorted of moving liquid column below.
Preferably, the center of the bottom of the filtrate head is a concave structure matched with the conical adsorption head.
Preferably, the sealing head is of a conical structure, and the sealing table is of a funnel-shaped structure matched with the sealing head.
Preferably, the upper end of the adsorption column is also provided with a limiting head, and the peripheral arm of the liquid moving column is provided with a limiting rib I which is matched with the limiting head and is positioned below the limiting head.
Preferably, the peripheral arm of the liquid moving column is also provided with a second limiting rib for limiting the liquid moving column from shaking left and right, and the second limiting rib is positioned between the first limiting rib and the sealing head.
Preferably, a liquid transferring pool is arranged at the opening of the upper end of the liquid transferring column.
Preferably, the filtrate head is detachably arranged at the lower end of the outer tube of the adsorption column.
Preferably, the filtrate head comprises a filtrate tank and a filtrate pipe, the filtrate pipe is positioned below the filtrate tank and is communicated with the filtrate tank, and the filtrate pipe is also provided with a clamping table.
The nucleic acid extraction adsorption column provided by the invention has the following beneficial technical effects:
(1) The invention sets the whole adsorption column as three parts of an adsorption column outer tube, an adsorption column and a liquid-moving column, the adsorption film is arranged below the adsorption column outer tube, the liquid-moving column can directly transfer nucleic acid solution into the adsorption column, the nucleic acid solution enters the adsorption column outer tube through an adsorption hole on the adsorption column, the adsorption hole is sealed by the liquid-moving column downwards, the adsorption column and the liquid-moving column form a piston structure similar to an injector, the nucleic acid solution in the adsorption column outer tube is pushed to complete adsorption through the adsorption film below the adsorption column outer tube, the whole steps can be realized through the up-and-down movement of the adsorption column and the liquid-moving column, the realization mode is single, the operation by a mechanical device is convenient, and the automatic operation of nucleic acid extraction is realized;
(2) The arrangement of the liquid transferring pool with a larger opening can ensure that the sample addition is more convenient;
(3) The adsorption film is arranged in the filtrate head, and the filtrate head is arranged in a detachable mode, so that the replacement is convenient.
Drawings
FIG. 1 is a schematic view of an assembled structure of the present invention;
FIG. 2 is a schematic view of the structure of the present invention prior to assembly;
FIG. 3 is a schematic view of the structure of the outer tube of the adsorption column and the filtrate head of FIG. 1;
FIG. 4 is a schematic structural view of an adsorption column;
FIG. 5 is a schematic view of a pipetting column;
in the figure, an adsorption column outer tube, a filtrate head 2, an adsorption column 3, a liquid transfer column 4, a filtrate tank 21, a filtrate tube 22, a clamping table 23, an adsorption head 31, an adsorption hole 32, a sealing sleeve 33, an adsorption tube 34, a positioning head 35, a liquid transfer tank 41, a pipette 42, a sealing head 43, a liquid transfer hole 44, a first 45 limiting rib and a second 46 limiting rib are arranged.
Detailed Description
The principles and features of the present invention are described below with reference to the drawings, the examples are illustrated for the purpose of illustrating the invention and are not to be construed as limiting the scope of the invention.
As shown in fig. 1 and 2, a nucleic acid extraction adsorption column comprises an adsorption column outer tube 1, a filtrate head 2, an adsorption column 3 and a liquid transfer column 4, wherein the filtrate head 2 is arranged at the lower end of the adsorption column outer tube 1 and is internally provided with an adsorption film, and the specific arrangement mode of the adsorption film can be seen in the prior art and is not described in detail herein; the lower end of the adsorption column 3 is positioned in the adsorption column outer tube 1 and can move up and down relative to the adsorption column outer tube 1, a sealing sleeve 33 is sleeved on the adsorption column 3, and an adsorption hole 32 through which nucleic acid passes is arranged at the lower end of the adsorption column 3; the pipetting column 4 comprises a pipette 42 with an opening at the upper end, a sealing head 43 capable of blocking the adsorption hole 32 at the lower end of the adsorption column 3 is arranged at the lower end of the pipette 42, a plurality of pipetting holes 44 are further arranged on the pipette 42, the maximum diameter of the sealing head 43 is smaller than the inner diameter of the adsorption column 3 so as to ensure that reagents in the pipette 42 can enter the adsorption column 3 through gaps between the sealing head 33 and the adsorption column 3, and when the sealing head 43 blocks the adsorption column hole 32, a sealing structure is formed between the adsorption column 3 and the inner tube of the outer tube 1 of the adsorption column through a sealing sleeve 33 so as to provide pressure for liquid in the outer tube 1 of the adsorption column.
The application method of the invention is as follows: the reagent is added into the pipette 42, as the sealing head 43 below the pipette 42 blocks the adsorption hole 32 on the adsorption column 3, the reagent stays in the pipette 42, the liquid-moving column 4 is pulled upwards, the sealing head 43 is separated from the adsorption hole 32, the reagent in the pipette 42 sequentially passes through the liquid-moving hole 44 and the adsorption hole 32 to enter the filtrate head 2 and the adsorption column outer tube 1, and then the adsorption column 3 is pulled upwards, so that the sealing head 43 blocks the adsorption hole 32, the adsorption column 3, the liquid-moving column 4 and the sealing sleeve 33 form a piston structure similar to an injector, the adsorption column 3 and the liquid-moving column 4 are pressed down at the same time, and the reagent flows out through the filtrate head 2 under the action of pressure to finish the extraction adsorption process.
As shown in fig. 3, the adsorption column 3 includes an adsorption head 31, an adsorption tube 34, an adsorption hole 32 and a sealing table, the adsorption head 31 is located at the lower end of the adsorption tube 34 and is in a conical structure, a sealing sleeve 33 is sleeved on the adsorption head 31, specifically, the sealing sleeve is arranged on the adsorption head 31 according to the principle and the arrangement method similar to that of an injector, the adsorption hole 32 is located on the adsorption head 31, the sealing table matched with the sealing head 43 below the liquid-moving column 4 is arranged at the bottom below the adsorption tube 34, and the adsorption hole 32 and the inner wall of the adsorption tube 34 are smoothly transited through the sealing table, so that dead angles in the adsorption column 3 are ensured.
Specifically, the center of the bottom of the filtrate head 2 is a concave structure matched with the conical adsorption head 31, so that the nucleic acid can completely pass through the adsorption film on the filtrate head 2. The sealing head 43 is of a conical structure, the sealing table is of a funnel-shaped structure matched with the sealing table, the adsorption hole 32 is positioned in the middle of the sealing table, the liquid moving column 4 moves downwards until the sealing head 43 completely abuts against the sealing table and seals the adsorption hole 32, and the sealing structure can provide pressure for liquid between the adsorption column outer tube 1 and the filtrate head 2 between the bottom of the adsorption column 3 and the inner tube of the adsorption column outer tube 1.
In this embodiment, we have still set up spacing head 35 in the upper end of adsorption column 3, spacing rib one 45 has been set up on the outer circumference arm of liquid displacement column 4, spacing rib one 45 and spacing head 35 assorted and be located the below of spacing head 35 and avoid liquid displacement column 4 to be pulled out by the maloperation in from adsorption column 3 on the one hand, on the other hand can play the effect of firm liquid displacement column 4, specifically, spacing head 35 can be with the structure of adsorption column 3 integrated into one piece, also can install on adsorption column 3 through detachable mode (like screw thread mounting mode, joint mode etc.), the outside diameter of liquid displacement column 4 < the inside diameter of spacing head 35 < the outside diameter of spacing rib one 45, neither influence liquid displacement column 4 reciprocate, guarantee again can block spacing rib one 45. In addition, we have further set up a spacing muscle second 46 in addition on the liquid column 4, and spacing muscle second 46 is located between spacing muscle first 45 and the seal head 43, and spacing muscle first 45 and spacing muscle second 46 can fine avoid liquid column 4 to take place to rock about in adsorption column 3.
In this embodiment, as shown in fig. 5, a liquid-transferring pool 41 is further disposed at the opening at the upper end of the liquid-transferring column 4, and the liquid-transferring pool 41 may be of an integral structure with the liquid-transferring column 4, or may be detachably (e.g. by a screw-thread mounting manner, a clamping manner, etc.) mounted on the liquid-transferring column 4, and the opening of the liquid-transferring pool 41 is larger than the inner diameter of the liquid-transferring column 4, so that the liquid-transferring pool is convenient for sample loading, such as a funnel-shaped structure.
The filter liquid head 2 can be set to the structure with the outer tube 1 integrated into one piece of adsorption column, also can set to solitary detachable structure, in this embodiment, in order to improve the utilization efficiency of whole device, make things convenient for the change of filter liquid head 2, we install the lower extreme at the outer tube 1 of adsorption column with the detachable of filter liquid head 2, specifically, it can install the lower extreme at the outer tube 1 of adsorption column through the mode of screw installation, conveniently dismantle the change, also can block at the outer tube 1 lower extreme of adsorption column through the joint mode of block and flange.
In this embodiment, the filtrate head 2 includes a filtrate tank 21 and a filtrate pipe 22, the filtrate pipe 22 is located below the filtrate tank 21 and is communicated with the filtrate tank 21, and a clamping table 23 which is convenient for positioning and fixing when the whole device is used is further arranged on the filtrate pipe 22.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (8)
1. The nucleic acid extraction adsorption column is characterized by comprising an adsorption column outer tube (1), a filtrate head (2), an adsorption column (3) and a liquid-moving column (4), wherein the filtrate head (2) is positioned at the lower end of the adsorption column outer tube (1) and is internally provided with an adsorption film; the lower end of the adsorption column (3) is positioned in the adsorption column outer tube (1) and can move up and down relative to the adsorption column outer tube (1), a sealing sleeve (33) is sleeved on the adsorption column (3), and an adsorption hole (32) through which nucleic acid passes is formed in the lower end of the adsorption column (3); the pipetting column (4) comprises a pipette (42) with an opening at the upper end, a sealing head (43) capable of blocking the adsorption hole (32) at the lower end of the adsorption column (3) is arranged at the lower end of the pipette (42), a plurality of pipetting holes (44) are further arranged on the pipette (42), and the maximum diameter of the sealing head (43) is smaller than the inner diameter of the adsorption column (3);
the adsorption column (3) comprises an adsorption head (31), an adsorption pipe (34), an adsorption hole (32) and a sealing table, wherein the adsorption head (31) is located at the lower end of the adsorption pipe (34) and is of a conical structure, a sealing sleeve (33) is sleeved on the adsorption head (31), the adsorption hole (32) is located on the adsorption head (31), and the sealing table matched with the sealing head (43) below the liquid transferring column (4) is arranged at the bottom below the adsorption pipe (34).
2. The nucleic acid extraction adsorption column according to claim 1, wherein the center of the bottom of the filtrate head (2) is a concave structure matching with the conical adsorption head (31).
3. The nucleic acid extraction adsorption column according to claim 1, wherein the sealing head (43) has a conical structure and the sealing table has a funnel-shaped structure matching the conical structure.
4. The nucleic acid extraction adsorption column according to claim 1, wherein a limiting head (35) is further arranged at the upper end of the adsorption column, a limiting rib I (45) is arranged on the peripheral arm of the liquid displacement column (4), and the limiting rib I (45) is matched with the limiting head (35) and is positioned below the limiting head (35).
5. The nucleic acid extraction adsorption column according to claim 4, wherein a second limit rib (46) for limiting the lateral shaking of the pipetting column (4) is further provided on the outer peripheral arm of the pipetting column (4), and the second limit rib (46) is located between the first limit rib (45) and the sealing head (43).
6. The nucleic acid extraction adsorption column according to claim 1, wherein a liquid-transferring bath (41) is provided at an opening at an upper end of the liquid-transferring column (4).
7. The nucleic acid extraction adsorption column according to claim 1, wherein the filtrate header (2) is detachably mounted at the lower end of the outer tube (1) of the adsorption column.
8. The nucleic acid extraction adsorption column according to claim 1, wherein the filtrate head (2) comprises a filtrate tank (21) and a filtrate pipe (22), the filtrate pipe (22) is located below the filtrate tank (21) and is communicated with the filtrate tank (21), and a clamping table (23) is further arranged on the filtrate pipe (22).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910804185.4A CN110437980B (en) | 2019-08-28 | 2019-08-28 | Nucleic acid extraction adsorption column |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910804185.4A CN110437980B (en) | 2019-08-28 | 2019-08-28 | Nucleic acid extraction adsorption column |
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| Publication Number | Publication Date |
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| CN110437980A CN110437980A (en) | 2019-11-12 |
| CN110437980B true CN110437980B (en) | 2023-12-22 |
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| CN201910804185.4A Active CN110437980B (en) | 2019-08-28 | 2019-08-28 | Nucleic acid extraction adsorption column |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003159077A (en) * | 2001-11-28 | 2003-06-03 | Fuji Photo Film Co Ltd | Method for separating and purifying nucleic acid and unit for separating and purifying nucleic acid |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN205275579U (en) * | 2015-12-29 | 2016-06-01 | 北京索莱宝科技有限公司 | Nucleic acid isolation purification kit subassembly |
| CN108841777A (en) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | The extracting method and device of extracellular vesica based on Electrostatic Absorption and its content |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2015073485A (en) * | 2013-10-09 | 2015-04-20 | セイコーエプソン株式会社 | Nucleic acid amplification method, device for nucleic acid extraction, cartridge for nucleic acid amplification reaction, and kit for nucleic acid amplification reaction |
-
2019
- 2019-08-28 CN CN201910804185.4A patent/CN110437980B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003159077A (en) * | 2001-11-28 | 2003-06-03 | Fuji Photo Film Co Ltd | Method for separating and purifying nucleic acid and unit for separating and purifying nucleic acid |
| CN104059849A (en) * | 2013-07-12 | 2014-09-24 | 中国人民解放军疾病预防控制所 | Device and method used for rapid extraction of nucleic acid |
| CN205275579U (en) * | 2015-12-29 | 2016-06-01 | 北京索莱宝科技有限公司 | Nucleic acid isolation purification kit subassembly |
| CN108841777A (en) * | 2018-06-22 | 2018-11-20 | 北京恩泽康泰生物科技有限公司 | The extracting method and device of extracellular vesica based on Electrostatic Absorption and its content |
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| CN110437980A (en) | 2019-11-12 |
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