CN205275579U - Nucleic acid isolation purification kit subassembly - Google Patents
Nucleic acid isolation purification kit subassembly Download PDFInfo
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- CN205275579U CN205275579U CN201521111969.2U CN201521111969U CN205275579U CN 205275579 U CN205275579 U CN 205275579U CN 201521111969 U CN201521111969 U CN 201521111969U CN 205275579 U CN205275579 U CN 205275579U
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Abstract
The utility model discloses a nucleic acid isolation purification kit subassembly has solved among the prior art transfer process from the kit to the purification tubular column and need introduce other quantitative instruments to control access to the volume of the solution in the adsorption column, the adsorption column of waste liquid collecting pipe inside is located to the complicated problem of just introducing impurity easily of this process, its technical scheme main points including the circular kit of upper and lower open -ended, waste liquid collecting pipe and unsettled card, the bottom of circular kit and joint in the upper shed of adsorption column are provided with on the upper shed of circular kit and are used for ration extrusion and sealed the advancing device of kit inner space has reached and has depended merely on the quantitative technological effect that shifts in liquid to the adsorption column of self structure realization.
Description
Technical field
This utility model relates to biomedicine field, more specifically, it relates to one is applied to adsorption column method extracts the genomic kit device of DNA in whole blood.
Background technology
The separation purification of nucleic acid is molecular biological important component part, is the important pre-treatment link of the various experimentations such as PCR, enzyme action, DNA sequencing downstream, in other words, and the success or failure of the quality of Nucleic acid quality directly decision follow-up test. Traditional separate nucleic acid method of purification is phenol-chloroform extracting nucleic acid, and the shortcoming that this method is extracted is inevitably to have the pollution of the impurity such as protein, saccharide, salt and organic solvent; Another kind of relatively conventional nucleic acid purification method is exactly adsorption column method, and this method operating process is:
(1) first, the PB1 solution taking a certain amount of blood sample and corresponding amount adds in test kit, and shakes about 1 minute;
(2) solution one step up adds the PB2 solution with PB1 solution even, again shakes a period of time;
(3) part for the liquid in step 2 being injected in purification tubing string, this part includes adsorption column and two parts of waste collection pipe are socketed to form, and solution joins the adsorption column inner chamber being set in waste collection pipe;
(4) it is placed on centrifuge by this purification tubing string to carry out the centrifugal treating time of about 1 minute, the adsorption column within taking out, and cleans out the waste liquid in waste collection pipe;
(5) adding PB3 solution in solution one step up, recentrifuge processes, and cleans out the waste liquid in waste collection pipe;
(6) adding PB4 solution in solution one step up, recentrifuge processes, and cleans out the waste liquid in waste collection pipe;
(7) material centrifugal treating that above-mentioned steps is obtained, that be stored in purification tubing string about 3 minutes, and adsorption column is taken out;
(8) after storage being had centrifugal treating, the adsorption column of material is mounted in another fluid collection tube, a small amount of PB5 solution is added in adsorption column, centrifugal treating about 1 minute, the liquid being now stored in fluid collection tube is nucleic acid extraction liquid, is the experimental subject of subsequent experimental process.
In said process, step 4 needs repeatedly to be centrifuged purification tubing string processing to step 7, there are two problems in this process, and one is that adsorption column easily departs from waste collection pipe under the influence of centrifugal force when centrifugal treating; Two is that adsorption column is smaller, and the amount of internal liquid can cause the amount that can affect again final nucleic acid extract centrifugal insufficient, few at most. The applying date is 2009.6.16, application number be 200920076350.0 utility model patent (hereinafter referred to as documents 1) disclosed in a kind of separate nucleic acid adsorption column, including adsorption column, waste collection pipe, adsorption column is enclosed within waste collection pipe, adsorption layer is placed in bottom adsorption column, is provided with the buckle fixing adsorption layer in adsorption column. When purification tubing string is repeatedly centrifuged processing by step 4 that what it mainly solved is to step 7, it is easy to the problem of the waste collection pipe that the adsorption column occurred departs from.
In documents 1, yet unresolved issue is the quantitative problem of liquid in adsorption column, say, that the transfer process from test kit to purification tubing string needs to introduce other quantitative instruments, to control to enter the amount of the solution in adsorption column, to ensure the effect of centrifugal treating. Not only process comparatively bothers, and the cleaning requirement of instrument is also comparatively strict, once less cleaning position can affect the effect of final nucleic acid extraction liquid.
Utility model content
For the deficiency that prior art exists, the purpose of this utility model is in that to provide one without introducing quantitative instrument, can to depend merely on self structure and realize quantitative transfer liquid to the separate nucleic acid purified reagent case assembly in adsorption column.
For achieving the above object, this utility model provides following technical scheme: a kind of separate nucleic acid purified reagent case assembly, include the circular reagent box of upper and lower opening, waste collection pipe and the unsettled adsorption column being arranged within waste collection pipe, it is characterized in that: the bottom of described circular reagent box and the upper shed being connected in adsorption column, the upper shed of circular reagent box is provided with the propulsion plant for quantitatively extruding and seal described test kit inner space.
By adopting technique scheme, by circular reagent box and adsorption column clamping, adsorption column is connected in again in waste collection pipe, so, then in the process of nucleic acid purification, instrument without introducing any outside can realize the transfer of liquid, particularly in the propulsion plant arranged in the open top of circular reagent box, by the movement of propulsion plant, thus the inner space of quantitative extruding circular reagent box, thus utilizing the gas pressure of propelling that the liquid quantitative within circular reagent box is clamp-oned adsorption column inner chamber, complete the content of the first seven step in background technology, whole process is without measuring equipment, depend merely on self structure and can realize the purpose of quantitative transfer liquid.
This utility model is further arranged to: being provided with draw-in groove on the outer round surface in described circular reagent cassette bottom portion, on described adsorption column, corresponding position is provided with and the fin of draw-in groove size fit.
By adopting technique scheme, owing to needing repeatedly to be centrifuged processing in the process of extracting nucleic acid, therefore, what adsorption column and circular reagent cassette bottom portion utilized draw-in groove and fin structure cooperatively forms more close clamping relation, is conducive to raising clamping intensity between the two in centrifugal treating process.
This utility model is further arranged to: described circular reagent box lower end is that inverted cone is arranged, and described draw-in groove is arranged on inverted cone face size and the position of fin size fit.
By adopting technique scheme, due in the configuration process of nucleic acid extraction liquid, the interior amount of liquid of generally rounded test kit is significantly larger than the amount of the liquid being squeezed in adsorption column, and, bottom opening due to circular reagent box, in order to prevent internal liquid from flowing into when not compact in adsorption column, less, bigger to give internal liquid one the small damping power that therefore bottom opening of circular reagent box to do. Therefore, circular reagent cassette bottom portion is arranged to the structure of inverted cone when being conducive to propulsion plant extruding circular reagent box inner chamber, internal liquid is extruded, it is prevented that the liquid in circular reagent cassette bottom portion difficultly flows in adsorption column.
This utility model is further arranged to: described propulsion plant is threadeded with the upper shed of circular reagent box.
By adopting technique scheme, the pitch of helicitic texture itself is certain value, therefore, threaded pitch has been known at the beginning of design, this value can directly as the quantitative criterion of propulsion plant, namely the relation of rotating cycle and the change of circular reagent box cavity volume is directly calculated by the nominal diameter of pitch, screw thread, thus realizing the quantitative of propulsion plant simply, accurately.
This utility model is further arranged to: the position connect with waste collection pipe clamp on described adsorption column is provided with snap ring, described waste collection tube cavity correspondence position is joined and is provided with groove, when described adsorption column is connected in waste collection pipe, interference fit between described snap ring and groove.
By adopting technique scheme, owing to adsorption column is placed on centrifugation apparatus when waste collection Guan Li newly processes together with circular reagent box, therefore, clamping relation between the two also wants reliable, the snap ring form that coordinate really up to the mark with groove, makes not only to possess between the two card relay, also has and is extruded, by snap ring, the pressure that groove brings, therefore, both engaging forces are more reliable.
This utility model is further arranged to: be provided with the graduation mark for labelling circle test kit internal liquid volume on the outer wall of described circular reagent box.
By adopting technique scheme, owing to the liquid in adsorption column is too much or the very few quality that all can affect last nucleic acid extraction liquid, therefore, being provided with of graduation mark is beneficial to the volume being accurately controlled the liquid being squeezed in adsorption column.
Accompanying drawing explanation
Fig. 1 is overall structure explosive view of the present utility model;
Fig. 2 is the structural representation of circular reagent box;
Fig. 3 is the assembly relation schematic diagram of waste collection pipe and adsorption column;
Fig. 4 is integrally-built assembling schematic diagram.
Accompanying drawing marks: 1, circular reagent box; 2, waste collection pipe; 3, adsorption column; 4, propulsion plant; 11, draw-in groove; 21, groove; 31, fin; 32, snap ring; 33, filter screen; 41, screw thread is connected.
Detailed description of the invention
Referring to figs. 1 through Fig. 4, this utility model embodiment is described further.
A kind of separate nucleic acid purified reagent case assembly, as it is shown in figure 1, include circular reagent box 1, waste collection pipe 2, adsorption column 3 and propulsion plant 4. wherein, the circular reagent box 1 column type in both ends open is arranged, its underpart is fixed on adsorption column 3, there is the upper end open place being clamped in waste collection pipe 2 lower end that adsorption column 3 has, three's clamping forms an entirety, when whole assembly prevents carrying out centrifugal treating on centrifuge, maintain the connection between three constant. propulsion plant 4 is arranged on the upper end open place of circular reagent box 1, when propulsion plant 4 moves down a segment distance, and the inner space of compression circular reagent box 1 that can be quantitative. describe known in background technology, liquid in circular reagent box 1 is the liquid of first stage prepared by nucleic acid extraction liquid, after the PB solution of centrifugal treating and addition different PH, need to be transferred to the adsorption treatment carrying out nucleic acid in adsorption column 3, and adsorption column 3 is being also required to through repeatedly centrifugal treating, therefore, should be within the specific limits by the amount of the liquid being transferred in adsorption column 3 in circular reagent box 1, too much can cause centrifugal treating difficulty, the very few amount that can affect again last nucleic acid extraction liquid, therefore, namely the function of the propulsion plant 4 in the present embodiment is for being pushed in adsorption column 3 by the liquid in circular reagent box 1 quantitatively.
Specifically, as in figure 2 it is shown, concrete structure and annexation to circular reagent box 1 and propulsion plant 4 do detailed narration. owing to the amount of the liquid in circular reagent box 1 is significantly larger than the amount of the liquid entered in adsorption column 3, therefore, cylinder is made on the top of circular reagent box 1, the diameter of upper shed is also greater than the diameter of adsorption column 3 at that time, its lower end to carry out clamping with adsorption column 3, therefore, in the connecting tube being provided with one section of inverted cone between the upper and lower ends, adaptive with adsorption column 3 in order to be contracted to by lower ending opening diameter first, another is then also for the liquid in convenient extrusion circular reagent box 1. the upper end open of circular reagent box 1 is in propulsion plant 4 and is connected by one section of connection screw thread 41, advantage of this is that: screw thread can manufacture according to standard value, now, the pitch connecting screw thread 41 manufactured, nominal diameter equivalence is fixing and known, therefore, when quantitatively mobile propulsion plant 4, can according to the number of turns of value rotary propelling device 4 correspondence set in advance, to complete the quantitative transfer of liquid in circular reagent box 1, in addition, owing to assembly to pass through this centrifugal treating, therefore, connect the type of attachment of screw thread 41 also to possess and certain put mold release function.
Further, with reference to Fig. 3, the assembly relation of adsorption column 3 with waste collection pipe 2 is specifically addressed. a filter screen 33 is included in adsorption column 3, flow in waste collection pipe 2 for the waste liquid when adsorption column 3 is centrifuged processing, the inside of filter screen 33 clamping and adsorption column 3 is near the position of bottom, waste collection pipe 2 is socketed on outside adsorption column 3, in order to strengthen both annexations, the outer wall of adsorption column 3 is arranged with a snap ring 32, accordingly, correspondence position in waste collection pipe 2 is provided with a groove 21, when both clampings, snap ring 32 is pressed in groove 21, interference annexation is formed between the two by the elastic deformation of snap ring 32, ensure will not get loose both when vertical new process. additionally, in order to ensure the clamping relation between circular reagent box 1 and adsorption column 3, be provided with fin 31 on adsorption column 3, when circular reagent box 1 snaps in adsorption column 3, fin 31 is conducive to the card relay of both enhancings.
Closer, as shown in Figure 4, being provided with the draw-in groove 11 corresponding to fin 31 on the taper connection face of circular reagent box 1, both form the clamping relation of circular reagent box 1 and adsorption column 3. Propulsion plant 4 is connected with circular reagent box 1 by connecting screw thread 41. It is to be noted, the type of attachment of propulsion plant 4 and circular reagent box 1 has multiple, as long as what can realize both knits the upper shed that line slides and can seal circular test kit 1 relatively, in order to control the amount promoted easily, graduation mark can be set on the outer wall of circular reagent box, to ensure the amount of movement of propulsion plant 4.
The above is only preferred implementation of the present utility model, and protection domain of the present utility model is not limited merely to above-described embodiment, and all technical schemes belonged under this utility model thinking belong to protection domain of the present utility model. It should be pointed out that, for those skilled in the art, without departing from the some improvements and modifications under this utility model principle premise, these improvements and modifications also should be regarded as protection domain of the present utility model.
Claims (6)
1. a separate nucleic acid purified reagent case assembly, include the circular reagent box of upper and lower opening, waste collection pipe and the unsettled adsorption column being arranged within waste collection pipe, it is characterized in that: the bottom of described circular reagent box and the upper shed being connected in adsorption column, the upper shed of circular reagent box is provided with the propulsion plant for quantitatively extruding and seal described test kit inner space.
2. separate nucleic acid purified reagent case assembly according to claim 1, is characterized in that: being provided with draw-in groove on the outer round surface in described circular reagent cassette bottom portion, on described adsorption column, corresponding position is provided with and the fin of draw-in groove size fit.
3. separate nucleic acid purified reagent case assembly according to claim 2, is characterized in that: described circular reagent box lower end is that inverted cone is arranged, and described draw-in groove is arranged on inverted cone face size and the position of fin size fit.
4. the separate nucleic acid purified reagent case assembly according to claim 1 or 2 or 3, is characterized in that: described propulsion plant is threadeded with the upper shed of circular reagent box.
5. separate nucleic acid purified reagent case assembly according to claim 1, it is characterized in that: the position connect with waste collection pipe clamp on described adsorption column is provided with snap ring, described waste collection tube cavity correspondence position is joined and is provided with groove, when described adsorption column is connected in waste collection pipe, interference fit between described snap ring and groove.
6. separate nucleic acid purified reagent case assembly according to claim 1, is characterized in that: be provided with the graduation mark for labelling circle test kit internal liquid volume on the outer wall of described circular reagent box.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521111969.2U CN205275579U (en) | 2015-12-29 | 2015-12-29 | Nucleic acid isolation purification kit subassembly |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521111969.2U CN205275579U (en) | 2015-12-29 | 2015-12-29 | Nucleic acid isolation purification kit subassembly |
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| CN205275579U true CN205275579U (en) | 2016-06-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110437980A (en) * | 2019-08-28 | 2019-11-12 | 湖北微伞医疗科技有限公司 | A kind of nucleic acid extraction adsorption column |
| CN111286449A (en) * | 2020-04-01 | 2020-06-16 | 宁波艾捷康宁生物科技有限公司 | Integrated adding method and device for pretreatment reagent of biological sample |
-
2015
- 2015-12-29 CN CN201521111969.2U patent/CN205275579U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110437980A (en) * | 2019-08-28 | 2019-11-12 | 湖北微伞医疗科技有限公司 | A kind of nucleic acid extraction adsorption column |
| CN110437980B (en) * | 2019-08-28 | 2023-12-22 | 湖北微伞医疗科技有限公司 | Nucleic acid extraction adsorption column |
| CN111286449A (en) * | 2020-04-01 | 2020-06-16 | 宁波艾捷康宁生物科技有限公司 | Integrated adding method and device for pretreatment reagent of biological sample |
| CN111286449B (en) * | 2020-04-01 | 2023-10-03 | 宁波艾捷康宁生物科技有限公司 | Biological sample pretreatment reagent integrated adding method and device |
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