CN207575856U - Column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links - Google Patents
Column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links Download PDFInfo
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- CN207575856U CN207575856U CN201721670516.2U CN201721670516U CN207575856U CN 207575856 U CN207575856 U CN 207575856U CN 201721670516 U CN201721670516 U CN 201721670516U CN 207575856 U CN207575856 U CN 207575856U
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Abstract
The utility model discloses a kind of 24 holes for PAGE purifying C18 desalination links to cross column system, including system ontology, the system ontology sets 24 hole solid-phase extracting instruments, the system ontology sets 24 hole sample filtering shelfs, system ontology is equipped with reagent storage device, it is connected between the reagent storage device and 24 hole solid-phase extracting instruments by pipeline, the system ontology also sets up 24 hole sample loading shelfs and 24 hole sample collection shelfs, and the 24 hole sample loading shelf and 24 hole sample collection shelfs are arranged on the right side of 24 hole solid-phase extracting instruments；The utility model is by combining 24 hole Solid Phase Extraction machines, water pump, the filtering shelf of customization, customization waste liquid bottle, the reagent device customized and vacuum line system, establish a set of automanual column system unit for desalting excessively, pass through the speed of C18 columns using the negative pressure of vacuum principle of water pump come liquid with precise control, sample is adsorbed and is eluted more efficient, 24 holes can simultaneously 24 DNA samples of desalination, flux higher.
Description
Technical field
The utility model is related to DNA to synthesize field, and column is crossed in specifically a kind of 24 holes for PAGE purifying C18 desalination links
System.
Background technology
DNA synthesis generally includes three big steps：
1）The synthesis of target DNA sequence is completed on solid phase carrier, obtains crude product；
2）Crude product DNA is cut down from solid phase carrier, and take off the blocking group on DNA by ammonolysis reaction；
3）Purifying removes impurity and the DNA fragmentation of synthesis failure.
Wherein, it is a kind of more commonly used purification process that the poly- propionamides of PAGE are gel purified, utilizes different length
The charge of DNA institutes band is different, and migration velocity is different under the function of current, reaches long and short sequence separation, finally obtains complete object
Segment realizes the purpose of purifying.Basic process is as follows：
DNA crude products are loaded in poly- propionamide gel first, are separated by electrophoresis, then in the UV lamp target fragment
DNA taps rubber, and is put into after being impregnated in salting liquid, makes DNA separate outs from glue, finally cross column desalination by C18 pillars, obtain
The DNA high to purity.
Traditional desalination process crosses column using hand gun, and reagent is made to flow through C18 pillars, is pushed in a manner of positive pressure, no
Only flux is small, and efficiency is low, and flow control depends entirely on manual operation, influenced whether the effect of column purification, and caused matter
It measures unstable.
Invention content
The purpose of this utility model is to provide a kind of 24 holes for PAGE purifying C18 desalination links to cross column system, with
Solve the problems mentioned above in the background art.
To achieve the above object, the utility model provides following technical solution：
Column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links, and including system ontology, the system ontology is set
24 hole solid-phase extracting instruments are put, the system ontology sets 24 hole sample filtering shelfs, and system ontology is equipped with reagent storage device, institute
It states and is connected between reagent storage device and 24 hole solid-phase extracting instruments by pipeline, the system ontology also sets up 24 hole sample loadings
Shelf and 24 hole sample collection shelfs, the 24 hole sample loading shelf and 24 hole sample collection shelfs are arranged on 24 hole solid phase extractions
Take the right side of instrument.
As further program of the utility model：The left end setting vacuum waste liquid bottle of 24 hole solid-phase extracting instrument, institute
It states and vacuum line is equipped between 24 hole solid-phase extracting instruments and vacuum waste liquid bottle, lead between 24 hole solid-phase extracting instruments and vacuum waste liquid bottle
Cross vacuum line connection.
As further program of the utility model：The system ontology sets water pump, the water pump and vacuum waste liquid bottle
Inside connected by vacuum line.
As further program of the utility model：The 24 hole sample filtering shelf is arranged on 24 hole solid-phase extracting instruments
On, the C18 pillars on the 24 hole sample filtering shelf and 24 hole solid-phase extracting instruments correspond.
As the utility model further scheme：The reagent storage device be divided into 80% methanol solution device,
0.05M NaCl solutions device, pure water solution device and 60% methanol solution device.
The storage prior art is compared, and the beneficial effects of the utility model are：The utility model is extracted by 24 hole solid phase of combination
Machine, water pump, the filtering shelf of customization, customization waste liquid bottle, the reagent device customized and vacuum line system are taken, establishes a set of half
Column system unit for desalting is automatically crossed, passes through the speed of C18 columns, sample using the negative pressure of vacuum principle of water pump come liquid with precise control
Product adsorb and elution it is more efficient, 24 holes can simultaneously 24 DNA samples of desalination, flux higher.
Description of the drawings
Fig. 1 crosses the structure diagram of column system to purify 24 holes of C18 desalination links for PAGE.
In figure：1- water pumps, 2- vacuum waste liquid bottles, 3-24 holes solid-phase extracting instrument, 4-24 holes sample loading shelf, 5-24 holes sample
Product collect shelf, 6-24 holes sample filtering shelf, 7- reagent storage devices, 8- vacuum lines.
Specific embodiment
The technical solution of this patent is described in more detail With reference to embodiment.
Referring to Fig. 1, column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links, it is described including system ontology
System ontology sets 24 hole solid-phase extracting instruments 3, the left end setting vacuum waste liquid bottle 2 of 24 hole solid-phase extracting instrument 3,24 hole
It is equipped between 8,24 hole solid-phase extracting instrument 3 of vacuum line and vacuum waste liquid bottle 2 and leads between solid-phase extracting instrument 3 and vacuum waste liquid bottle 2
It crosses vacuum line 8 to connect, the system ontology sets water pump 1, and the inside of the water pump 1 and vacuum waste liquid bottle 2 passes through vacuum tube
Road 8 connects, and 24 hole solid-phase extracting instrument, 3 vacuum pressure, the system ontology setting are supplied to by vacuum line 8 by water pump 1
24 hole sample filtering shelfs 6, the 24 hole sample filtering shelf 6 are arranged on 24 hole solid-phase extracting instruments 3, the 24 hole sample mistake
C18 pillars on filter stand 6 and 24 hole solid-phase extracting instruments 3 of son correspond, and system ontology is equipped with reagent storage device 7, the examination
It is connected between 7 and 24 hole solid-phase extracting instrument 3 of agent storage device by pipeline, the reagent storage device 7 divides for 80% methanol solution
Device, 0.05M NaCl solutions device, pure water solution device and 60% methanol solution device, the system ontology also set up 24 holes
Sample loading shelf 4 and 24 hole sample collection shelfs 5, the 24 hole sample loading shelf 4 and 24 hole sample collection shelfs 5 are set
On the right side of 24 hole solid-phase extracting instruments 3.
The operation principle of the utility model：
Water pump 1 is supplied to 24 hole solid-phase extracting instrument, 3 vacuum pressure by vacuum line 8, in 24 hole sample loading shelfs 4
Sample removes blob of viscose by 24 hole sample filtering shelfs 6 and flows on 24 hole solid-phase extracting instrument, 3 corresponding C18 pillars, corresponding examination
Agent is added to by each reagent storage device 7 in C18 pillars, and 24 hole sample collection shelfs 5 are put into 24 hole solid-phase extracting instruments 3
Collect last sterling.
1st, the primer for preparing column is placed on 24 hole sample loading shelfs 4；
2nd, the reagent that 80% methanol of certain volume is opened in the columns such as boost valve is added to drip at a slow speed entirely in each C18 pillars
It drains in portion；
3rd, in each C18 pillars plus the 0.5M sodium chloride of certain volume, reagent in the columns such as boost valve is opened at a slow speed
Drop is all drained；
4th, 24 hole sample filtering shelfs 6 are put on a cl 8 column, and primer salting liquid one-to-one correspondence is transferred on C18 columns；
5th, 24 hole sample loading shelfs 4 were placed in column machine, open the reagent in the columns such as boost valve drip at a slow speed until
All drain；
6th, the reagent in each pillar plus in the columns such as the pure water of certain volume, opening boost valve is quickly dripped until whole
It drains；
7th, clean plastics recovery pipe one-to-one correspondence is placed on 5 on 24 sample well collecting frames, be put into column machine；
8th, add 60% methanol of certain volume in C18 columns, open the reagents such as boost pressure valve and drip at a slow speed until whole
It drains, primer is eluted in 5ml centrifuge tubes at this time；
9th, 24 hole sample collection shelfs 5 are taken out.
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the utility model can
To be combined with each other.
In the description of the present invention, it is to be appreciated that term " " center ", " longitudinal direction ", " transverse direction ", " on ", " under ",
The orientation or position relationship of the instructions such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the utility model and simplifies description rather than instruction
Or imply that signified device or element there must be specific orientation, with specific azimuth configuration and operation, therefore be not understood that
For the limitation to the utility model.In addition, term " first ", " second " etc. are only used for description purpose, and it is not intended that instruction
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic." first ", " second " etc. are defined as a result,
Feature can express or implicitly include one or more this feature.It is in the description of the present invention, unless another
It is described, " multiple " are meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or integrally
Connection；Can be mechanical connection or electrical connection；It can be directly connected, can also be indirectly connected by intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, on being understood by concrete condition
State concrete meaning of the term in the utility model.
The better embodiment of this patent is explained in detail above, but this patent is not limited to above-mentioned embodiment party
Formula, can also be under the premise of this patent objective not be departed from the knowledge that one skilled in the relevant art has
It makes a variety of changes.
Claims (5)
1. column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links, including system ontology, which is characterized in that described
System ontology sets 24 hole solid-phase extracting instruments（3）, 24 hole sample filtering shelfs of the system ontology setting（6）, system ontology sets
There is reagent storage device（7）, the reagent storage device（7）With 24 hole solid-phase extracting instruments（3）Between connected by pipeline, it is described
System ontology also sets up 24 hole sample loading shelfs（4）With 24 hole sample collection shelfs（5）, the 24 hole sample loading shelf
（4）With 24 hole sample collection shelfs（5）It is arranged on 24 hole solid-phase extracting instruments（3）Right side.
2. column system is crossed in 24 holes according to claim 1 for PAGE purifying C18 desalination links, which is characterized in that institute
State 24 hole solid-phase extracting instruments（3）Left end setting vacuum waste liquid bottle（2）, 24 hole solid-phase extracting instrument（3）With vacuum waste liquid bottle
（2）Between be equipped with vacuum line（8）, 24 hole solid-phase extracting instruments（3）With vacuum waste liquid bottle（2）Between pass through vacuum line（8）Even
It connects.
3. column system is crossed in 24 holes according to claim 1 for PAGE purifying C18 desalination links, which is characterized in that institute
State system ontology setting water pump（1）, the water pump（1）With vacuum waste liquid bottle（2）Inside pass through vacuum line（8）Connection.
4. column system is crossed in 24 holes according to claim 1 for PAGE purifying C18 desalination links, which is characterized in that institute
State 24 hole sample filtering shelfs（6）It is arranged on 24 hole solid-phase extracting instruments（3）On, the 24 hole sample filtering shelf（6）With 24 holes
Solid-phase extracting instrument（3）On C18 pillars correspond.
5. column system is crossed in 24 holes according to claim 1 for PAGE purifying C18 desalination links, which is characterized in that institute
State reagent storage device（7）It is divided into 80% methanol solution device, 0.05M NaCl solutions device, pure water solution device and 60% methanol
Solution containment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721670516.2U CN207575856U (en) | 2017-12-05 | 2017-12-05 | Column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721670516.2U CN207575856U (en) | 2017-12-05 | 2017-12-05 | Column system is crossed in a kind of 24 holes for PAGE purifying C18 desalination links |
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| Publication Number | Publication Date |
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| CN207575856U true CN207575856U (en) | 2018-07-06 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112378726A (en) * | 2021-01-18 | 2021-02-19 | 南京仪汇仪器设备有限公司 | A solid-phase extraction device for food detects |
-
2017
- 2017-12-05 CN CN201721670516.2U patent/CN207575856U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112378726A (en) * | 2021-01-18 | 2021-02-19 | 南京仪汇仪器设备有限公司 | A solid-phase extraction device for food detects |
| CN112378726B (en) * | 2021-01-18 | 2021-04-23 | 南京仪汇仪器设备有限公司 | A solid-phase extraction device for food detects |
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| CP03 | Change of name, title or address |
Address after: Building 23, No. 99, Shuhai Road, Songjiang District, Shanghai, 201611 Patentee after: Briggs Biotechnology (Shanghai) Co.,Ltd. Address before: Room 6045, Building 1, No. 5500, Yuanjiang Road, Minhang District, Shanghai, 201111 Patentee before: SHANGHAI BIOLIGO BIOTECHNOLOGY CO.,LTD. |