CN212246988U

CN212246988U - Anionic polymer solid phase extraction column

Info

Publication number: CN212246988U
Application number: CN202020428179.1U
Authority: CN
Inventors: 孙国威; 阮必武; 穆宁
Original assignee: Wuxi Galak Chromatographic Technology Co ltd
Current assignee: Wuxi Galak Chromatographic Technology Co ltd
Priority date: 2020-03-30
Filing date: 2020-03-30
Publication date: 2020-12-29
Anticipated expiration: 2030-03-30

Abstract

The utility model relates to a plasmid DNA rapid extraction device, anion polymer solid phase extraction post specifically says so. The device is characterized by comprising a vertically arranged cavity column tube, wherein a filter cartridge is arranged in the cavity column tube, the outer diameter of the filter cartridge is smaller than the inner diameter of the cavity column tube, and filter holes are uniformly distributed on the filter cartridge. An upper sieve plate and a lower sieve plate which are horizontally arranged are sequentially arranged in the cavity column tube below the filter cartridge, sieve pores are uniformly distributed on the upper sieve plate and the lower sieve plate, the peripheries of the upper sieve plate and the lower sieve plate are in sealing fit with the inner wall of the cavity column tube, and an anion polymerization filler is filled between the upper sieve plate and the lower sieve plate. The solid phase extraction column has good stability and high adsorption capacity under alkaline conditions.

Description

Anionic polymer solid phase extraction column

Technical Field

The utility model relates to a plasmid DNA rapid extraction device, anion polymer solid phase extraction post specifically says so.

Background

Plasmid DNA is an important object in modern biological and medical research, and the isolation and purification of plasmid DNA are required for the research of nucleic acid structure and function, genetic engineering, protein engineering, etc. The solid phase extraction is a technology for extracting and separating analytes in a liquid sample by taking a solid material as an adsorbent, has the advantages of high enrichment factor, low solvent consumption, simplicity in operation and the like, and is very suitable for enrichment and detection of trace analytes in a complex sample. Solid phase extraction is generally achieved by means of a solid phase extraction column.

At present, most of the traditional solid phase extraction columns adopt porous silica gel materials as adsorbents. However, the silica gel material has poor stability under alkaline conditions, and the silica gel material has generally small pore diameter (6-30 nm), so that the adsorption amount is low when the silica gel material is used for purifying biomacromolecules such as DNA and the like.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to provide an anionic polymer solid phase extraction post, this solid phase extraction post is stable good under alkaline condition, and the adsorption capacity is higher.

In order to solve the problems, the following technical scheme is provided:

the utility model discloses an anionic polymer solid phase extraction post's characteristics are including the vertical cavity column tube of arranging, have the cartridge filter in the cavity column tube, and the external diameter of cartridge filter is less than the internal diameter of cavity column tube, and the equipartition has the filtration pore on the cartridge filter. An upper sieve plate and a lower sieve plate which are horizontally arranged are sequentially arranged in the cavity column tube below the filter cartridge, sieve pores are uniformly distributed on the upper sieve plate and the lower sieve plate, the peripheries of the upper sieve plate and the lower sieve plate are in sealing fit with the inner wall of the cavity column tube, and an anion polymerization filler is filled between the upper sieve plate and the lower sieve plate.

Wherein the anionic polymerization filler is formed by polymerizing tertiary amine and polystyrene-divinylbenzene filler.

The filter cartridge is concentrically arranged with the cavity column pipe, and the cylinder opening of the filter cartridge extends out of the upper end of the cavity column pipe.

The lower end of the cavity column tube is concentrically connected with a conical tube, the outer diameter of the upper end of the conical tube is the same as that of the cavity column tube, and the lower end of the conical tube is concentrically connected with a liquid outlet tube.

By adopting the scheme, the method has the following advantages:

because the utility model discloses an anionic polymer solid phase extraction post has the cartridge filter in the cavity column tube, has upper screen plate and lower screen plate in the cavity column tube of cartridge filter below, and the packing has anionic polymerization to pack between upper screen plate and the lower screen plate. The anionic polymeric filler acts as an adsorbent and has excellent chemical stability at a pH of 1-14. Moreover, the anion polymerization filler can obtain over 400nm of super-macropores by controlling the synthesis conditions, and the adsorbing capacity of the adsorbent with the super-macropores to DNA and other biological macromolecules is higher.

Drawings

FIG. 1 is a schematic structural diagram of an anionic polymer solid phase extraction column of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

As shown in figure 1, the utility model discloses an anionic polymer solid phase extraction column includes vertical cavity column pipe 1 of arranging, has cartridge filter 2 in the cavity column pipe 1, and the external diameter of cartridge filter 2 is less than the internal diameter of cavity column pipe 1, and the equipartition has the filtration pore on the cartridge filter 2. An upper sieve plate 3 and a lower sieve plate 5 which are horizontally arranged are sequentially arranged in a cavity column tube 1 below the filter cartridge 2, sieve holes are uniformly distributed on the upper sieve plate 3 and the lower sieve plate 5, the peripheries of the upper sieve plate 3 and the lower sieve plate 5 are in sealing fit with the inner wall of the cavity column tube 1, and an anion polymerization filler 4 is filled between the upper sieve plate 3 and the lower sieve plate 5.

The anionic polymerization filler 4 is formed by polymerizing tertiary amine and polystyrene-divinylbenzene filler.

The filter cartridge 2 and the cavity column tube 1 are concentrically arranged, and the opening of the filter cartridge 2 extends out of the upper end of the cavity column tube 1.

The lower extreme of cavity column pipe 1 is connected with conical duct 6 with one heart, and the upper end external diameter of conical duct 6 is the same with the external diameter of cavity column pipe 1, and the lower extreme of conical duct 6 is connected with drain pipe 7 with one heart.

When plasmid DNA was extracted, overnight cultured cells were collected, centrifuged, and the supernatant was discarded. Then, adding lysis solution into cells, lysing the cells by mild shaking, adding neutralizing solution, and uniformly mixing for neutralization. Then, the equilibrium liquid is added into a filter cylinder 2 in the solid phase extraction column, and is infiltrated and balanced, and the equilibrium liquid is drained by the action of gravity. And then, uniformly mixing the neutralized sample, adding the uniformly mixed sample into a filter cylinder 2 of the solid-phase extraction column, allowing the sample filtered by the filter cylinder 2 to enter a filler of the solid-phase extraction column, adsorbing, and discarding the filtrate. Thereafter, the filtration tube and the packing were rinsed with the equilibration solution, and the filtrate was discarded. After that, the filter cartridge 2 is removed, the extraction cartridge is rinsed with washing liquid, and the filtrate is discarded. And finally, adding the eluent into the extraction column, standing, and collecting filtrate, namely plasmid DNA.

Claims

1. An anion polymer solid phase extraction column is characterized by comprising a cavity column tube (1) which is vertically arranged, wherein a filter cartridge (2) is arranged in the cavity column tube (1), the outer diameter of the filter cartridge (2) is smaller than the inner diameter of the cavity column tube (1), and filter holes are uniformly distributed on the filter cartridge (2); the filter cartridge is characterized in that an upper sieve plate (3) and a lower sieve plate (5) which are horizontally arranged are sequentially arranged in a cavity column tube (1) below the filter cartridge (2), sieve meshes are uniformly distributed on the upper sieve plate (3) and the lower sieve plate (5), the periphery of the upper sieve plate (3) and the periphery of the lower sieve plate (5) are in sealed fit with the inner wall of the cavity column tube (1), and an anion polymerization filler (4) is filled between the upper sieve plate (3) and the lower sieve plate (5).

2. The solid phase extraction column of claim 1, wherein said anionic polymeric filler (4) is polymerized from a tertiary amine and a polystyrene-divinylbenzene filler.

3. The solid phase extraction column of anionic polymers as claimed in claim 1, characterized in that the filter cartridge (2) is arranged concentrically with the hollow column (1), and the mouth of the filter cartridge (2) protrudes from the upper end of the hollow column (1).

4. The solid-phase extraction column of anionic polymers as claimed in any one of claims 1 to 3, characterized in that the lower end of the hollow column tube (1) is concentrically connected with a conical tube (6), the outer diameter of the upper end of the conical tube (6) is the same as that of the hollow column tube (1), and the lower end of the conical tube (6) is concentrically connected with a liquid outlet tube (7).