CN210834377U - Automatic QuECHERS purifier - Google Patents
Automatic QuECHERS purifier Download PDFInfo
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- CN210834377U CN210834377U CN201921078068.6U CN201921078068U CN210834377U CN 210834377 U CN210834377 U CN 210834377U CN 201921078068 U CN201921078068 U CN 201921078068U CN 210834377 U CN210834377 U CN 210834377U
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Abstract
The utility model discloses an automatic QuECHERS purifier, include as follows: sample extraction pipe, detachable joint, needle type SPE purifying column, SPE purifying column filler, power pump, filter, the purifying tube, No. 1 silica gel pipe fitting, No. 2 silica gel pipe fittings, No. 3 silica gel pipe fittings, No. 4 silica gel pipe fittings, the sample extraction pipe passes through No. 1 silica gel union coupling is in needle type SPE purifying column front end on the detachable joint, the power pump passes through No. 2 silica gel pipe fittings and connects needle type SPE purifying column rear end on the detachable joint, the filter passes through No. 3 silica gel pipe fittings and connects after the power pump, the purifying tube pass through No. 4 silica gel pipe fittings with the filter is connected. The QuECHERS purification device is simple, convenient and quick to operate, and greatly improves the efficiency of detection work.
Description
Technical Field
The utility model relates to pretreatment technical field, in particular to automatic QuECHERS purifier.
Background
QuEChERS is developed by the American academy of agriculture for agriculture in 2003, is a rapid, simple and convenient pretreatment technology which is newly developed internationally in recent years and can provide high-quality agricultural product detection, and is widely applied to detection of pesticide residues in fruit, vegetable and grain samples. Currently, the QuEChERS method essentially comprises two procedures, extraction and purification. The operation flow is as follows: adding an extracting agent and extraction salt into a centrifugal tube, uniformly mixing, adding a crushed sample, vibrating and uniformly mixing, putting the centrifugal tube into a centrifugal machine, separating an upper organic phase after centrifugation into an equal part, adding a solid phase adsorbent for purification, adding magnesium sulfate to remove residual moisture in an extract, centrifuging, and taking a supernatant for analysis. Although the QuEChERS method is generally applied, the method has the defects that a centrifugal device is required to be used for the second time in the sample extraction and purification so as to separate the supernatant from the sample and the purifying agent, and the operation is complicated; before the sample is injected into the kind and detected at liquid matter, need filter once more for avoiding the influence of large granule to testing result and mass spectrum life.
Aiming at the defects of the prior QuEChERS pretreatment technology, it is necessary to develop a QuEChERS pretreatment method which is simple and convenient to operate and rapid.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an easy and simple to handle, quick, automatic QuEChERS pretreatment method.
Therefore, the utility model adopts the following technical proposal,
an automated queechers purification device comprising the following: the device comprises a sample extraction tube, a detachable connector, a needle type SPE purification column, SPE purification column filler, a power pump, a filter, a purification tube, a No. 1 silica gel tube, a No. 2 silica gel tube, a No. 3 silica gel tube and a No. 4 silica gel tube; SPE purifying column both ends by detachable articulate, the sample extraction pipe passes through No. 1 silicone tube is connected needle type SPE purifying column front end on the detachable joint, the power pump is connected through No. 2 silica gel pipe fittings needle type SPE purifying column rear end on the detachable joint, the filter is connected through No. 3 silica gel pipe fittings after the power pump, the purifying tube through No. 4 silica gel pipe fittings with the filter is connected.
Preferably, be provided with the sieve in the needle type SPE purification column, the number of sieve is 2, SPE purification column packs and fills in the needle type SPE purification column between the sieve.
Preferably, the SPE purification column packing is one or more of anhydrous MgSO4, PSA, GCB or C18.
Preferably, the power pump is a syringe pump or a peristaltic pump.
Preferably, a filter membrane is arranged in the filter, the filter membrane is made of polyether sulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the pore diameter of the filter membrane is 0.22 μm or 0.45 μm.
Preferably, the structure of the purification tube is a pointed-bottom column or a round-bottom column, the volume of the purification tube is 10mL, 15mL or 20mL, and the purification tube is made of polypropylene, polyethylene, polycarbonate or glass.
Preferably, the number of the needle type SPE purification columns is 1 or 2-3, and the needle type SPE purification columns are connected in series.
The utility model provides an automatic QuECHERS purifier, through the power pump with the sample extract from sample extraction tube to needle type SPE purification column to the power pump can aspirate repeatedly, make the extract purified repeatedly many times in SPE purification column, improved purification efficiency and saved the depurative quantity; the centrifugal step is not needed, the purification step is combined in multiple steps, and time and labor are saved. Especially, when GC/LC-MS detection is carried out, the filtered purified liquid can be directly put on a computer for analysis, and the detection working efficiency is greatly improved.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Detailed Description
In order to make the objects, features and advantages of the present invention clearer, embodiments of the present invention are described in more detail below with reference to the accompanying drawings and examples, and in the following description, many specific details are set forth to provide a thorough understanding of the present invention, but the present invention can be implemented in many other ways than those described. Therefore, the present invention is not limited by the specific embodiments disclosed below.
Example 1
An automated queechers purification device comprising the following: the device comprises a sample extraction tube 1, a detachable connector 2, a needle type SPE purification column 3, SPE purification column filler 4, a power pump 5, a filter 6, a purification tube 7, a No. 1 silica gel pipe fitting 10, a No. 2 silica gel pipe fitting 20, a No. 3 silica gel pipe fitting 30 and a No. 4 silica gel pipe fitting 40; needle type SPE purifying column both ends by detachable 2 connect, sample extraction tube 1 passes through No. 1 silicone tube 10 is connected needle type SPE purifying column 3 front end detachable 2 is last, power pump 5 is connected through No. 2 silica gel pipe fittings 20 needle type SPE purifying column 3 rear end detachable 2 is last, filter 6 is connected through No. 3 silica gel pipe fittings 30 power pump 5 is later, purifying tube 7 through No. 4 silica gel pipe fittings 40 with filter 6 connects.
Wherein, be provided with sieve 50 in the needle type SPE purification column 3, the number of sieve 50 is 2, needle type SPE purification column filler 4 is filled in the needle type SPE purification column 3 between the sieve 50.
Wherein, the needle type SPE purification column packing 4 is one or more of anhydrous MgSO4, PSA, GCB or C18.
Wherein, the power pump 5 is an injection pump or a peristaltic pump.
The filter 6 is internally provided with a filter membrane, the filter membrane is made of polyether sulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the pore diameter of the filter membrane is 0.22 μm or 0.45 μm.
The structure of the purification tube 7 is a pointed-bottom column or a round-bottom column, the volume of the purification tube 7 is 10mL, 15mL or 20mL, and the purification tube 7 is made of polypropylene, polyethylene, polycarbonate or glass.
Wherein, the number of the needle type SPE purification columns 3 is 1.
Example 2
An automated queechers purification device comprising the following: the device comprises a sample extraction tube 1, a detachable connector 2, a needle type SPE purification column 3, SPE purification column filler 4, a power pump 5, a filter 6, a purification tube 7, a No. 1 silica gel pipe fitting 10, a No. 2 silica gel pipe fitting 20, a No. 3 silica gel pipe fitting 30 and a No. 4 silica gel pipe fitting 40; needle type SPE purifying column both ends by detachable 2 connect, sample extraction tube 1 passes through No. 1 silicone tube 10 is connected needle type SPE purifying column 3 front end detachable 2 is last, power pump 5 is connected through No. 2 silica gel pipe fittings 20 needle type SPE purifying column 3 rear end detachable 2 is last, filter 6 is connected through No. 3 silica gel pipe fittings 30 power pump 5 is later, purifying tube 7 through No. 4 silica gel pipe fittings 40 with filter 6 connects.
Wherein, be provided with sieve 50 in the needle type SPE purification column 3, the number of sieve 50 is 2, needle type SPE purification column filler 4 is filled in the needle type SPE purification column 3 between the sieve 50.
Wherein, the needle type SPE purification column packing 4 is one or more of anhydrous MgSO4, PSA, GCB or C18.
Wherein, the power pump 5 is an injection pump or a peristaltic pump.
The filter 6 is internally provided with a filter membrane, the filter membrane is made of polyether sulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the pore diameter of the filter membrane is 0.22 μm or 0.45 μm.
The structure of the purification tube 7 is a pointed-bottom column or a round-bottom column, the volume of the purification tube 7 is 10mL, 15mL or 20mL, and the purification tube 7 is made of polypropylene, polyethylene, polycarbonate or glass.
Wherein, the number of needle type SPE purification column 3 is 2, 3 connected modes of needle type SPE purification column are series connection.
Example 3
An automated queechers purification device comprising the following: the device comprises a sample extraction tube 1, a detachable connector 2, a needle type SPE purification column 3, SPE purification column filler 4, a power pump 5, a filter 6, a purification tube 7, a No. 1 silica gel pipe fitting 10, a No. 2 silica gel pipe fitting 20, a No. 3 silica gel pipe fitting 30 and a No. 4 silica gel pipe fitting 40; needle type SPE purifying column both ends by detachable 2 connect, sample extraction tube 1 passes through No. 1 silicone tube 10 is connected needle type SPE purifying column 3 front end detachable 2 is last, power pump 5 is connected through No. 2 silica gel pipe fittings 20 needle type SPE purifying column 3 rear end detachable 2 is last, filter 6 is connected through No. 3 silica gel pipe fittings 30 power pump 5 is later, purifying tube 7 through No. 4 silica gel pipe fittings 40 with filter 6 connects.
Wherein, be provided with sieve 50 in the needle type SPE purification column 3, the number of sieve 50 is 2, needle type SPE purification column filler 4 is filled in the needle type SPE purification column 3 between the sieve 50.
Wherein, the needle type SPE purification column packing 4 is one or more of anhydrous MgSO4, PSA, GCB or C18.
Wherein, the power pump 5 is an injection pump or a peristaltic pump.
The filter 6 is internally provided with a filter membrane, the filter membrane is made of polyether sulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the pore diameter of the filter membrane is 0.22 μm or 0.45 μm.
The structure of the purification tube 7 is a pointed-bottom column or a round-bottom column, the volume of the purification tube 7 is 10mL, 15mL or 20mL, and the purification tube 7 is made of polypropylene, polyethylene, polycarbonate or glass.
Wherein, the number of needle type SPE purification column 3 is 3, 3 connection modes of needle type SPE purification column are series connection.
During operation, power pump 5 pumps the sample to needle type SPE purifying column 3, through the mode of pumping repeatedly, makes the sample in needle type SPE purifying column 3 is repeated many times and is purified, and the sample after purifying passes through filter 6 filters and enters into in purifying tube 7. Meanwhile, by adjusting the flow speed and the direction of the power pump 5, the sample is always kept in a certain section of the position range of the silicone tube 10, so that the sample does not enter the sample extraction tube 1, and the purification efficiency of the sample is improved. The whole pretreatment process does not need to additionally use centrifugal equipment, is quick, is convenient to operate, and improves the efficiency of detection work.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (7)
1. An automatic QuECHERS purification device, characterized by comprising the following: the device comprises a sample extraction tube, a detachable connector, a needle type SPE purification column, SPE purification column filler, a power pump, a filter, a purification tube, a No. 1 silica gel tube, a No. 2 silica gel tube, a No. 3 silica gel tube and a No. 4 silica gel tube; needle type SPE purifying column both ends by detachable articulate, the sample extraction pipe passes through No. 1 silicone tube is connected needle type SPE purifying column front end on the detachable articulate, the power pump is connected through No. 2 silica gel pipe fittings needle type SPE purifying column rear end on the detachable articulate, the filter passes through No. 3 silica gel pipe fittings and connects after the power pump, the purifying tube pass through No. 4 silica gel pipe fittings with the filter is connected.
2. The automatic QuECHERS purifying device according to claim 1, wherein sieve plates are arranged in the needle-type SPE purifying column, the number of the sieve plates is 2, and the SPE purifying column packing is filled between the sieve plates in the needle-type SPE purifying column.
3. The automated QuECHERS purification device according to claim 1, wherein the SPE purification cartridge packing is one or more of anhydrous MgSO4, PSA, GCB or C18.
4. The automated QuECHERS purification device according to claim 1, wherein the powered pump is a syringe pump or a peristaltic pump.
5. The automatic QuECHERS purification device according to claim 1, wherein a filter membrane is arranged in the filter, the filter membrane is made of polyethersulfone, polytetrafluoroethylene or polyvinylidene fluoride, and the pore size of the filter membrane is 0.22 μm or 0.45 μm.
6. The automated QuECHERS purification device according to claim 1, wherein the purification tube has a structure of a pointed-bottom column or a round-bottom column, the volume of the purification tube is 10mL, 15mL or 20mL, and the purification tube is made of polypropylene, polyethylene, polycarbonate or glass.
7. The automatic QuECHERS purifying device according to claim 1, wherein the number of the needle-type SPE purifying columns is 1 or 2-3, and the needle-type SPE purifying columns are connected in series.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921078068.6U CN210834377U (en) | 2019-07-10 | 2019-07-10 | Automatic QuECHERS purifier |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921078068.6U CN210834377U (en) | 2019-07-10 | 2019-07-10 | Automatic QuECHERS purifier |
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| CN210834377U true CN210834377U (en) | 2020-06-23 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112630087A (en) * | 2020-12-14 | 2021-04-09 | 锐德检测技术(天津)有限公司 | Method for determining fat in food |
| US20220120721A1 (en) * | 2020-10-16 | 2022-04-21 | Ningbo Municipal Center for Disease Control and Prevention | Kit and method for determination of fentanyl drugs in biological samples |
-
2019
- 2019-07-10 CN CN201921078068.6U patent/CN210834377U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220120721A1 (en) * | 2020-10-16 | 2022-04-21 | Ningbo Municipal Center for Disease Control and Prevention | Kit and method for determination of fentanyl drugs in biological samples |
| CN112630087A (en) * | 2020-12-14 | 2021-04-09 | 锐德检测技术(天津)有限公司 | Method for determining fat in food |
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