CN214991469U - Nucleic acid extraction device for manual magnetic bead adsorption - Google Patents
Nucleic acid extraction device for manual magnetic bead adsorption Download PDFInfo
- Publication number
- CN214991469U CN214991469U CN202023257107.4U CN202023257107U CN214991469U CN 214991469 U CN214991469 U CN 214991469U CN 202023257107 U CN202023257107 U CN 202023257107U CN 214991469 U CN214991469 U CN 214991469U
- Authority
- CN
- China
- Prior art keywords
- nucleic acid
- acid extraction
- hollow pipe
- stick
- magnetic bead
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 55
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 55
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 55
- 238000000605 extraction Methods 0.000 title claims abstract description 42
- 239000011324 bead Substances 0.000 title claims abstract description 39
- 238000001179 sorption measurement Methods 0.000 title claims description 32
- 230000002745 absorbent Effects 0.000 claims abstract description 5
- 239000002250 absorbent Substances 0.000 claims abstract description 5
- 229920003023 plastic Polymers 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 15
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000000034 method Methods 0.000 description 13
- 230000009089 cytolysis Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000005406 washing Methods 0.000 description 4
- 238000010828 elution Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000007885 magnetic separation Methods 0.000 description 2
- 238000009739 binding Methods 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000001821 nucleic acid purification Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Abstract
The utility model relates to a be used for absorbent nucleic acid extraction element of manual magnetic bead, including centrifuging tube, tube cap and absorption stick, the tube cap center runs through and is fixed with hollow pipe box, hollow pipe box upper end opening, and the lower extreme seals, and inside the hollow pipe box is inserted to absorption stick detachable, the absorption stick includes handle, connecting rod and magnetic force stick, and absorption stick upper end is equipped with the handle, and the handle below is equipped with the connecting rod, and the connecting rod end is equipped with the magnetic force stick. The utility model provides a be used for absorbent nucleic acid extraction element of manual magnetic bead, the operation need not use experimental facilities such as automatic nucleic acid extraction appearance or magnetic force frame, has simplified the nucleic acid and has drawed the flow, greatly reduced experiment cost, and experimenter convenient operation is swift, is worth using widely.
Description
Technical Field
The utility model relates to a nucleic acid extraction technical field, concretely relates to be used for absorbent nucleic acid extraction element of manual magnetic bead.
Background
In the fields of modern molecular biology research, clinical disease diagnosis, blood transfusion, food safety detection and the like, the extraction of bioactive substances such as cells, proteins, nucleic acids and the like plays an important role. Among them, a bioactive substance compound in which nucleic acid is polymerized from many nucleotides is one of the most basic substances of life. The purification method of nucleic acid in nucleic acid extraction is mainly divided into two types, one is a column adsorption method, and the other is a magnetic bead adsorption method. The principle of the magnetic bead adsorption method is that magnetic beads are specifically identified and efficiently combined with nucleic acid molecules on a micro interface, and compared with an over-column adsorption method, the magnetic bead adsorption method is simpler and easier to implement in an experimental process, so that the magnetic bead adsorption method is widely applied. The existing implementation forms of nucleic acid extraction by a magnetic bead adsorption method are divided into two types of manual extraction and automatic instrument extraction, wherein the automatic instrument extraction needs to purchase the automatic nucleic acid extractor, and the instrument cost is tens of thousands yuan; manual extraction generally requires the procurement of magnetic racks, which are also thousands of dollars in cost, both operations are relatively costly to implement, and the process is relatively complex.
Therefore, how to design a nucleic acid extraction device that can extract nucleic acid at low cost, conveniently and quickly is a problem that needs to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to prior art's defect and not enough, provide a be used for the adsorbed nucleic acid extraction element of manual magnetic bead, can accomplish the nucleic acid extraction operation on the basis that does not rely on experimental facilities such as automatic nucleic acid extraction appearance or magnetic force frame, convenient and fast reduces nucleic acid purification cost.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a nucleic acid extraction element for manual magnetic bead is absorbent, including centrifuging tube, tube cap and absorption stick, the tube cap center runs through and is fixed with hollow pipe box, hollow pipe box upper end opening, the lower extreme seals, it is liftoff insertible to adsorb stick detachable inside the hollow pipe box, it includes handle, connecting rod and magnetic force stick to adsorb the stick, it is equipped with the handle to adsorb the stick upper end, the handle below is equipped with the connecting rod, the connecting rod end is equipped with the magnetic force stick.
Further, the outer wall of the upper end of the centrifugal tube is provided with an external thread.
Further, the inner wall of the pipe cover is provided with internal threads.
Can increase the sealing performance of the extraction device and avoid the overflow of nucleic acid, etc.
Furthermore, the hollow pipe sleeve is made of medical plastics.
The medical plastic has good chemical inertness and biological safety.
Further, the length of the hollow pipe sleeve below the pipe cover is greater than or equal to two thirds of the length of the centrifugal pipe.
Further, the length of the adsorption rod is greater than or equal to that of the hollow pipe sleeve.
The combination of the adsorption rod and the hollow pipe sleeve can fully adsorb the magnetic beads.
Compared with the prior art, the utility model discloses following superiority has:
insert the hollow pipe box with the absorption stick, can make the magnetic bead adsorb in hollow pipe box bottom, the magnetic bead adsorbs can also follow the combination of hollow pipe box and absorption stick and shift after accomplishing, it takes out from the hollow pipe box to adsorb the stick, the magnetic bead can drop in the centrifuge tube, it need not use experimental facilities such as automatic nucleic acid extraction appearance or magnetic frame to operate like this, nucleic acid extraction process has been simplified, this a nucleic acid extraction device for manual magnetic bead is adsorbed simple structure, and reasonable design, greatly reduced experiment cost, experimenter convenient operation is swift, be worth using widely.
Drawings
FIG. 1 is a schematic structural diagram of the nucleic acid extraction apparatus of the present invention.
FIG. 2 is a schematic diagram of the nucleic acid extracting apparatus according to the present invention, when it is used for manual magnetic bead adsorption.
In the figure: 1. a tube cover; 2. centrifuging the tube; 11. a hollow pipe sleeve; 3. an adsorption rod; 31. a handle; 32. a connecting rod; 33. a magnetic bar; 4. a common centrifuge tube cap; 5. a nucleic acid; 6. magnetic beads.
Detailed Description
The technical solutions in the embodiments of the present invention are described below clearly and completely, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example 1
A nucleic acid extraction element for manual magnetic bead is adsorbed, including centrifuging tube 2, tube cap 1 and absorption stick 3, 1 center of tube cap is run through and is fixed with hollow pipe box 11, 11 upper end openings of hollow pipe box, and the lower extreme seals, and 3 detachable inserts hollow pipe box 11 insidely of absorption stick, and absorption stick 3 includes handle 31, connecting rod 32 and magnetic force stick 33, and 3 upper ends of absorption stick are equipped with handle 31, and the handle 31 below is equipped with connecting rod 32, and the connecting rod 32 end is equipped with magnetic force stick 33.
This embodiment can insert the hollow pipe box with adsorbing the stick, make the magnetic bead adsorb in hollow pipe box bottom, the magnetic bead adsorbs can also follow the combination of hollow pipe box and adsorption stick and shift after accomplishing, take out from the hollow pipe box the adsorption stick, the magnetic bead can drop in the centrifuge tube, the operation need not use experimental facilities such as automatic nucleic acid extraction appearance or magnetic frame like this, the nucleic acid extraction procedure has been simplified, nucleic acid extraction device simple structure, reasonable in design, greatly reduced experiment cost, the experimenter convenient operation is swift, be worth using widely.
Example 2
A nucleic acid extraction element for manual magnetic bead is adsorbed, including centrifuging tube 2, tube cap 1 and absorption stick 3, 1 center of tube cap is run through and is fixed with hollow pipe box 11, 11 upper end openings of hollow pipe box, and the lower extreme seals, and 3 detachable inserts hollow pipe box 11 insidely of absorption stick, and absorption stick 3 includes handle 31, connecting rod 32 and magnetic force stick 33, and 3 upper ends of absorption stick are equipped with handle 31, and the handle 31 below is equipped with connecting rod 32, and the connecting rod 32 end is equipped with magnetic force stick 33.
Further, the outer wall of the upper end of the centrifugal tube 2 is provided with an external thread.
Further, the inner wall of the tube cover 1 is provided with internal threads.
Can increase the sealing performance of the extraction device and avoid the overflow of nucleic acid, etc.
Further, the hollow pipe sleeve 11 is made of medical plastics, specifically PP, PE or PVC.
The medical plastic has good chemical inertness and biological safety.
Further, the length of the hollow pipe sleeve 11 below the pipe cover 1 is greater than or equal to two thirds of the length of the centrifugal pipe 2.
Further, the length of the adsorption rod 3 is greater than or equal to that of the hollow pipe sleeve 11.
The combination of the adsorption rod and the hollow pipe sleeve can fully adsorb the magnetic beads.
This embodiment is through the further design to nucleic acid extraction element structure, material, size, makes nucleic acid extraction element's leakproofness better, and the magnetic bead adsorption effect obtains improving.
Application method
As shown in FIG. 2, the manual magnetic bead adsorption nucleic acid extraction process comprises lysis, binding, washing and elution. When the nucleic acid extraction device of the embodiment performs manual magnetic bead adsorption operation, a biological sample is firstly placed in a centrifuge tube 2, lysis solution is added to enter a lysis link, a common centrifuge tube cover 4 is arranged on the centrifuge tube to ensure complete sealing of lysis, the common centrifuge tube cover 4 is the prior art, nucleic acid 5 is released after the lysis link is completed, magnetic beads 6 are added at the moment, the common centrifuge tube cover 4 is replaced by the tube cover 1 of the embodiment to enter a combination link, an adsorption rod 3 is inserted into a hollow tube sleeve 11 after the combination link is completed, so that the tail end of the hollow tube sleeve 11 adsorbs a combination of the nucleic acid 5 and the magnetic beads 6, the combination of the hollow tube sleeve 11 and the adsorption rod 3 is taken out together after the adsorption is completed, waste liquid is removed, the combination is transferred into a cleaned centrifuge tube 2, the combination of the nucleic acid 5 and the magnetic beads 6 is transferred together due to adsorption at the tail end of the hollow tube sleeve 11, and then the combination enters a washing I, II, taking out the adsorption rod 3 to enable the combination of the nucleic acid 5 and the magnetic bead 6 to fall into the centrifuge tube 2, adding washing liquid I, point-vibrating for a plurality of times, then repeating the magnetic separation operation of the combination link, taking out the adsorption rod 3 to enable the combination of the nucleic acid 5 and the magnetic bead 6 to fall into the centrifuge tube 2, then adding washing liquid II, point-vibrating for a plurality of times, then repeating the magnetic separation operation of the combination link, entering an elution link, taking out the adsorption rod 3 to enable the combination of the nucleic acid 5 and the magnetic bead 6 to fall into the centrifuge tube 2, adding eluent to enable the nucleic acid 5 to be separated from the combination, inserting the adsorption rod 3 into the hollow tube sleeve 11 after the elution link is completed, enabling the tail end of the hollow tube sleeve 11 to adsorb the magnetic bead 6, taking out the combination of the hollow tube sleeve 11 and the adsorption rod 3 after the adsorption is completed, taking out the magnetic bead 6 together due to be adsorbed at the tail end of the hollow tube sleeve 11, putting the common centrifugal tube cover 4 back to the centrifuge tube 2, finally, purified nucleic acid 5 is obtained.
In conclusion, this a nucleic acid extraction device for manual magnetic bead is adsorbed simple structure, reasonable in design need not use experimental facilities such as automatic nucleic acid extraction appearance or magnetic frame, greatly reduced experiment cost has simplified the nucleic acid and has drawed the process, and experimenter convenient operation is swift, is worth using widely.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is familiar with the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered in the protection scope of the present invention.
Claims (6)
1. A nucleic acid extraction element for manual magnetic bead is absorbent, including centrifuging tube, tube cap and adsorption rod, its characterized in that: the pipe cap center runs through and is fixed with hollow pipe box, hollow pipe box upper end opening, the lower extreme is sealed, adsorb the inseparable insertible inside the hollow pipe box, it includes handle, connecting rod and magnetic rod to adsorb the stick, it is equipped with the handle to adsorb the stick upper end, the handle below is equipped with the connecting rod, the connecting rod end is equipped with the magnetic rod.
2. The nucleic acid extraction apparatus according to claim 1, characterized in that: the outer wall of the upper end of the centrifugal tube is provided with external threads.
3. The nucleic acid extraction apparatus according to claim 1, characterized in that: the inner wall of the pipe cover is provided with internal threads.
4. The nucleic acid extraction apparatus according to claim 1, characterized in that: the hollow pipe sleeve is made of medical plastics.
5. The nucleic acid extraction apparatus according to claim 1, characterized in that: the length of the hollow pipe sleeve below the pipe cover is more than or equal to two thirds of the length of the centrifugal pipe.
6. The nucleic acid extraction apparatus according to claim 1, characterized in that: the length of the adsorption rod is greater than or equal to that of the hollow pipe sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023257107.4U CN214991469U (en) | 2020-12-29 | 2020-12-29 | Nucleic acid extraction device for manual magnetic bead adsorption |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023257107.4U CN214991469U (en) | 2020-12-29 | 2020-12-29 | Nucleic acid extraction device for manual magnetic bead adsorption |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214991469U true CN214991469U (en) | 2021-12-03 |
Family
ID=79142161
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023257107.4U Expired - Fee Related CN214991469U (en) | 2020-12-29 | 2020-12-29 | Nucleic acid extraction device for manual magnetic bead adsorption |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214991469U (en) |
-
2020
- 2020-12-29 CN CN202023257107.4U patent/CN214991469U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211203 |