CN200948422Y

CN200948422Y - Test tube for lymphocyte seperation

Info

Publication number: CN200948422Y
Application number: CN 200620014468
Authority: CN
Inventors: 刘志强; 谷学英; 刘翠云; 牛向兰; 何欢; 曾序春
Original assignee: Inst Of Population & Family Planning Sciences Shenzhen City
Current assignee: Inst Of Population & Family Planning Sciences Shenzhen City
Priority date: 2006-08-31
Filing date: 2006-08-31
Publication date: 2007-09-19
Anticipated expiration: 2016-08-31

Abstract

The utility model relates to a test tube for lymphocyte separation, comprising a test tube body. The inner peripheral wall at the middle part of the tube body is protruded inwards to form a bulge; the inner caliber of the bulge is less than the inner caliber of the upper and the lower part of the tube body. When the test tube of the utility model is used for lymphocyte separation, the lymphocyte is focused on the middle small layer surface; 30ml peripheral blood can be centrifuged in one time with two test tubes. The utility model has the advantages of high separation efficiency and convenient operation.

Description

Lymphocyte separates uses test tube

Technical field

The utility model relates to a kind of test tube, relates in particular to a kind of lymphocyte separation test tube.

Background technology

Agnogenic habitual abortion accounts for the 56%-72% of miscarriage, separation and purification patient husband or irrelevant individual lymphocyte, and it is efficient up to 70-86% to carry out active immunity treatment to the patient, is proved to be efficient ways.

The method of using active immunity treatment habitual abortion is also uncomplicated, meets difficulty sometimes but prepare husband's lymphocyte suspension, because of lymphocytic extraction is to realize according to the different principle of the proportion of various cells in the blood.Separating PBLC in the past, mainly is to be used for experiment to detect use, so the lymphocyte number that needs is little, uses the peripheral blood of 1-2ml to get final product, and generally uses the common sterilized test tube of common 10ml, and orifice area is less, can not feel difficulty during separation.And treatment habitual abortion needs number to reach 10 ⁷/ ml need separate from about 30ml blood, as use common 10ml test tube, once needs to use this kind test tube 8-15 to prop up, the experimental implementation effort, time-consuming that seems very, and separative efficiency is lower.

As use the test tube of large volume, as use the test tube of 50ml, there is a problem again, be exactly that the test tube sectional area is too big, because when operation experimenter need use glass pipette to draw lymphocyte from buffy coat, and the lymphocyte aspect is constantly rocked along with the stirring of suction pipe, so the lymphocyte sucking-off relatively will be required great effort, time-consuming, and can a large amount of unwanted other liquid, the operating procedures of increase subsequent experimental of testing of sucking-off.

The utility model content

Shortcomings such as technical problem to be solved in the utility model is to overcome the prior art medium size lymphocyte when separating, operating cost low with the test tube separative efficiency, effort provide a kind of separative efficiency height, lymphocyte easy and simple to handle to separate and use test tube.

For solving the problems of the technologies described above, the technical solution of the utility model is: provide a kind of lymphocyte to separate and use test tube, this test tube comprises body, and the internal perisporium at described body middle part is to projecting inward and form projection, and the interior bore of described projection is less than the interior bore of the upper and lower of body.

More specifically, described projection is arranged at inboard wall of test tube.

More specifically, described projection in the form of a ring, its longitudinal section is trapezoidal or arc.

More specifically, described projection is to be arranged at inboard wall of test tube by mode one-body molded or that paste.

More specifically, described projection is curved inwardly or is bent by the tube wall in the middle part of the test tube and forms.

More specifically, described projection is convexly curved or is trapezoidal bending.

Compared with prior art, the utlity model has following beneficial effect: designed a kind of special test tube and be specifically designed to the lymphocytic separation of larger volume, the upper and lower of relative aperture body is little in the medium position of this test tube, when using such test tube to carry out the lymphocyte zonal centrifugation, lymphocyte just is enriched on the less aspect in middle part, the 30ml peripheral blood is divided into two disposable centrifugal getting final product of test tube, uses the benefit of this test tube isolated lymphocytes to be embodied in the following aspects:

1, the minimizing number of operations is convenient and swift, and the separative efficiency height uses common test tube need be divided into 8-15 and props up, and uses test tube described in the utility model only to need 1-2 to prop up.

2, reduce opportunities for contamination, the test tube of use multioperation number of times more is many more, and it is big more to increase opportunities for contamination.

3, separative efficiency height uses test tube described in the utility model, because lymphocyte is enriched in the test tube middle part, and the middle part bore is less, just is easy to so draw buffy coat, and can not draw a large amount of other liquid.

4, use this test tube speed fast, reduce patient's stand-by period, increase the lymphocyte survival rate, because lymphocyte is long more in the external time, survival rate is low more, uses this test tube because convenient separation is quick, so reduced a large amount of experimental implementation time, thereby reduced lymphocyte in the external time, also reduced patient's stand-by period simultaneously.

Description of drawings

Fig. 1 shows schematic diagram for cuing open of the utility model first embodiment;

Fig. 2 shows schematic diagram for cuing open of the utility model second embodiment;

Fig. 3 shows schematic diagram for cuing open of the utility model the 3rd embodiment;

Fig. 4 shows schematic diagram for cuing open of the utility model the 4th embodiment.

The specific embodiment

With reference to shown in Figure 1, the utility model relates to a kind of lymphocyte separation test tube, it comprises body A, the internal perisporium at the middle part 2 of described body A is to projecting inward, and form projection 2, and described protruding 2 interior bore is less than the interior bore of top 3 and the bottom 1 of body A, and described projection can or be integrally formed on the inwall of test tube by the mode of pasting, its in the form of a ring, and the longitudinal section is trapezoidal.The bottom of test tube can be circular or square.When isolated lymphocytes, add lymphocyte separation medium, carry out density gradient centrifugation, lymphocyte is enriched in the middle part of test tube, the separation test operating personnel can be easily from the lymphocyte of the required analysis of middle part sucking-off.

The material of test tube of the present utility model can use transparent materials such as polystyrene or glass, makes test tube and projection with polystyrene material, can pack the back and adopt Co ⁶⁰Irradiation sterilization is done disposable use; As use glass material, the use of can sterilizing repeatedly.

Present embodiment is to be example to separate the 30ml peripheral blood, can design the serial test tube of a cover according to this mentality of designing, is used to separate the PBLC of different volumes from small to large, such as: 1ml, 2ml, 3ml, 4ml, 5ml ... until 50ml etc.

As shown in Figure 2, be second embodiment of the present utility model, only will be illustrated with the different piece of first embodiment, described lymphocyte separates with the longitudinal section of the projection 21 at test tube middle part curved.

As shown in Figure 3, be the 3rd embodiment of the present utility model, only will be illustrated with the different piece of first embodiment, the projection 22 that described lymphocyte separates with the test tube middle part is directly inwardly to be convexly curved to be out of shape by the tube wall in the middle part of the test tube to form.

As shown in Figure 4, be the 4th embodiment of the present utility model, only will be illustrated with the different piece of first embodiment, the projection 23 that described lymphocyte separates with the test tube middle part is directly inwardly to be trapezoidal bending distortion by the tube wall in the middle part of the test tube to form.

Claims

1, a kind of lymphocyte separates and use test tube, comprises the test tube body, it is characterized in that: the internal perisporium at described body middle part is to projecting inward and form protrudingly, and the interior bore of described projection is less than the interior bore of the upper and lower of body.

2, test tube is used in lymphocyte separation according to claim 1, and it is characterized in that: described projection is arranged at inboard wall of test tube.

3, lymphocyte according to claim 2 separates and uses test tube, it is characterized in that: described projection in the form of a ring, its longitudinal section is trapezoidal or arc.

4, use test tube according to claim 2 or 3 described lymphocytes separation, it is characterized in that: described projection is to be arranged at inboard wall of test tube by mode one-body molded or that paste.

5, test tube is used in lymphocyte separation according to claim 1, it is characterized in that: described projection is curved inwardly or is bent by the tube wall in the middle part of the test tube and forms.

6, test tube is used in lymphocyte separation according to claim 5, and it is characterized in that: described projection is convexly curved or is trapezoidal bending.