CN204710574U - A kind of fluid separation applications centrifuge tube - Google Patents

A kind of fluid separation applications centrifuge tube Download PDF

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Publication number
CN204710574U
CN204710574U CN201520243842.XU CN201520243842U CN204710574U CN 204710574 U CN204710574 U CN 204710574U CN 201520243842 U CN201520243842 U CN 201520243842U CN 204710574 U CN204710574 U CN 204710574U
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China
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pipe
centrifuge tube
lid
cylinder
fluid separation
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CN201520243842.XU
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Chinese (zh)
Inventor
侯莉莉
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Beijing Co Ltd Of Sinomenium Acutum Paddy Auspiciousness Stem Cell Technological Research Institute
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Beijing Co Ltd Of Sinomenium Acutum Paddy Auspiciousness Stem Cell Technological Research Institute
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Abstract

The utility model discloses a kind of fluid separation applications centrifuge tube, comprise body, be arranged on the pipe lid that body covers tightly by body upper end, and at the bottom of the pipe being movably connected on body lower end, described body lower end is provided with Drainage pipe, when described centrifuge tube is centrifugal, described Drainage pipe is placed at the bottom of pipe, and seals by the bottom of pipe; Described pipe covers the cylinder being provided with and connecting liquid-transfering gun, and described cylinder is hollow tubular structure, and is connected with body, described cylinder is stamped cap lid.The utility model is by arranging the syndeton at the bottom of pipe lid, body, pipe, to be fixedly connected on body at the bottom of pipe, the centrifugal work of liquid can be carried out, when needing the liquid needed for extraction, can separate at the bottom of pipe with body, discharging unwanted liquid from the Drainage pipe of body afterwards, when continuing the liquid needed for discharge, collecting.The function of pipette and centrifuge tube integrates by the utility model, can carry out the separation and Extraction of required cell fast, improve operating efficiency.

Description

A kind of fluid separation applications centrifuge tube
Technical field
The utility model relates to a kind of cell separation set, particularly relates to a kind of fluid separation applications centrifuge tube.
Background technology
In Bioexperiment, want the fluid separation applications after centrifugal to open, liquid sample loading gun or Pasteur pipe generally being selected to take out experiment need, abandons the unwanted liquid of experiment.But often occur that a phenomenon is exactly test the liquid needed to inhale when not coming up or drink up with the unwanted liquid of a lot of experiment in an experiment.Such as when doing PMNC and being separated, as shown in Figure 1, mononuclearcell is positioned at the position of intermediate layer b, and there are blood plasma and blood platelet in a place, upper strata, and there is parting liquid at c place of lower floor.When getting with liquid-transfering gun or Pasteur pipe, draw while intermediate layer and be often drawn to much blood plasma, need again centrifugal be separated, sometimes also can draw part parting liquid, because parting liquid is always in bottom, very large inconvenience can be caused to centrifugation below.
Operation also there will be a lot of problem so in addition, first sample loading gun and Pasteur pipe uptake once limited, often need repeatedly to draw the liquid that we want during experiment, not only waste time and energy, and if the interface of separating layer also can be destroyed when drawing the liquid of below separating layer.Secondly, repeatedly absorption can improve the pollution probability of some aseptic experiments, and the liquid drunk up is impure can affect subsequent experimental, increases subsequent experimental purification step.
Utility model content
The purpose of this utility model is to provide a kind of fluid separation applications centrifuge tube, the function of centrifuge tube and pipette is integrated, and by discharge orderly for unwanted liquid, can access the liquid of needs afterwards, substantially increase operating efficiency after centrifugation.
For reaching this object, the utility model is by the following technical solutions: a kind of fluid separation applications centrifuge tube, comprise body, be arranged on the pipe lid that body covers tightly by body upper end, and at the bottom of the pipe being movably connected on body lower end, described body lower end is provided with Drainage pipe, when described centrifuge tube is centrifugal, described Drainage pipe is placed at the bottom of pipe, and seals by the bottom of pipe; Described pipe covers the cylinder being provided with and connecting liquid-transfering gun, and described cylinder is hollow tubular structure, and is connected with body, described cylinder is stamped cap lid.
As preferably, described body lower end is provided with the taper tube wall extending to Drainage pipe, and upper end is provided with boss, being set on boss of described pipe lid sealing.
As preferably, described outer wall of boss and cap wall are provided with the screw thread of working in coordination, and screw tight pipe lid, and boss sealing covers tightly by described pipe lid.
As preferably, upper end at the bottom of described pipe is provided with the conical depression with taper tube wall mating shapes, described conical depression is provided with the screw thread of working in coordination with taper tube wall, screws at the bottom of pipe, at the bottom of described pipe, Drainage pipe is sealed while the coated described taper tube wall of conical depression.
As preferably, at the bottom of described pipe, inside is provided with sealing column, and described sealing column is fixedly installed on the bottom at the bottom of pipe, and when sealing Drainage pipe at the bottom of described pipe, described sealing column is inserted described Drainage pipe and Drainage pipe sealing filled.
As preferably, described Drainage pipe is circular tube structure, and along discharge direction diameter from large to small, and minimum diameter is identical with the diameter of sealing column.
As preferably, described cylinder and cap lid inwall are provided with the screw thread of working in coordination, and cylinder is covered tightly by threaded engagement by described cap lid.
As preferably, described body is provided with scale.
As preferably, the interface place of described cylinder bottom surface and pipe lid is provided with cruciform baffles agitated vessel, and described cruciform baffles agitated vessel is connected to cylindrical inwall and one-body molded with it; Described cruciform baffles agitated vessel is placed with sterile cotton, and described sterile cotton is full of cylindrical inside.
The beneficial effects of the utility model:
1, by arranging the syndeton at the bottom of pipe lid, body, pipe, to be fixedly connected on body at the bottom of pipe, the centrifugal work of liquid can be carried out, when needing the liquid needed for extraction, can separate at the bottom of pipe with body, discharging unwanted liquid from the Drainage pipe of body afterwards, when continuing the liquid needed for discharge, collecting.The function of pipette and centrifuge tube integrates by the utility model, can carry out the separation and Extraction of required cell fast, improve operating efficiency.
2, by arranging taper tube wall in body lower end, liquid can be allowed slowly to concentrate, being convenient to discharge and extract.
Accompanying drawing explanation
Fig. 1 is the liquid layered schematic diagram in the utility model background technology;
Fig. 2 is overall structure schematic diagram of the present utility model;
Fig. 3 is the structural representation of body of the present utility model;
Fig. 4 is the structural representation of pipe lid of the present utility model and cap lid;
Fig. 5 is the structural representation at the bottom of pipe of the present utility model;
In figure:
1, body; 2, Guan Gai; 3, at the bottom of pipe; 4, cap lid; 11, Drainage pipe; 12, taper tube wall; 13, boss; 21, cylinder; 22, cruciform baffles agitated vessel; 31, conical depression; 32, sealing column.
Detailed description of the invention
The technical solution of the utility model is further illustrated by detailed description of the invention below in conjunction with accompanying drawing.
In order to required liquid can be extracted fast after centrifugal, the utility model provides a kind of fluid separation applications centrifuge tube, as shown in Figure 2-5, it comprises body 1, be provided with the pipe lid 2 covered tightly by body 1 in the upper end of body 1, be connected with at the bottom of pipe 3 in the lower end of body 1, being meant to of described flexible connection: at the bottom of pipe, 3 are fixedly connected with body 1 when centrifuge tube of the present utility model carries out centrifugal separation, and when carrying out after being separated moving liquid extraction, be then separated with body 1.Drainage pipe 11 is provided with, for the discharge of liquid in body 1 lower end.When centrifuge tube of the present utility model carries out centrifugal, at the bottom of pipe, 3 are fixedly connected with body 1, and now Drainage pipe 11 to be placed at the bottom of pipe in 3, and by 3 sealing up at the bottom of pipe, so as centrifugal time there will not be the leakage of liquid; With reference to Fig. 4, pipe lid 2 is provided with the cylinder 21 connecting liquid-transfering gun, this cylinder 21 in hollow tubular structure, and is connected with body 1; The interface place of cylinder 21 bottom surface and pipe lid 2 is provided with cruciform baffles agitated vessel 22, this cruciform baffles agitated vessel 22 is placed with sterile cotton (not shown) and sterile cotton is full of the inside of whole cylinder 21.Concrete, this cruciform baffles agitated vessel 22 is connected to cylinder 21 inwall and one-body molded with it, thus can prevent from experiment from carrying out middle sterile cotton falling in body 1.And by the inside of sterile cotton filling disks cylinder 21, the air that liquid-transfering gun can be made to carry effectively is filtered, preventing pollution.Cylinder 21 is stamped cap lid 4, when carry out centrifugal or do not use liquid-transfering gun to move liquid time, cylinder 21 on pipe lid 2 tightly covers (being in sealing state) by cap lid 4, when carrying out when using liquid-transfering gun moving liquid, then takes off cap lid 4, liquid-transfering gun is connected with cylinder 21, after connecting liquid-transfering gun, just can disposable discharge liquid, for collection, eliminate the time of repeatedly drawing waste, improve the efficiency of extraction.
As shown in Figure 3, be also provided with the taper tube wall 12 extending to Drainage pipe 11 one end in the lower end of body 1, body 1 is connected with Drainage pipe 11 transition by this taper tube wall 12, and its pyramidal structure can make liquid slowly concentrate on the one hand, finally flows to Drainage pipe 11; Also to be convenient on the other hand at the bottom of pipe 3 with the connection of body 1.Boss 13 is provided with, for tube connector lid 2 in the upper end of body 1.
In the present embodiment, pipe lid 2 is being set on boss 13 of sealing.Concrete, be have the screw thread of working in coordination on the outer wall of boss 13 and the inwall of pipe lid 2, by screwing pipe lid 2, boss 13 sealing can slowly cover tightly by pipe lid 2.
As shown in Figure 5, at the bottom of pipe, the upper end of 3 is provided with the conical depression 31 with taper tube wall 12 mating shapes, and then 3 can be made at the bottom of pipe to coordinate closely with body 1.Same, in this conical depression 31 He on taper tube wall 12, be provided with the screw thread of working in coordination, be sealedly attached to taper tube wall 12 to make conical depression 31.By screwing at the bottom of pipe 3, the conical depression 31 on it is coated taper tube wall 12 gradually, meanwhile 3 to be sealed by Drainage pipe 11 at the bottom of pipe.
As preferred technical scheme, the structure sealed by Drainage pipe 11 is sealing column 32, to be arranged at the bottom of pipe 3 inner and be fixedly installed at the bottom of pipe 3 bottom, at the bottom of pipe, 3 when sealing Drainage pipe 11, and sealing column 32 is inserted Drainage pipe 11 and Drainage pipe 11 is sealed filling.Sealing column 32 fills Drainage pipe 11, can not affect the separation of parting liquid to cell on the one hand; Then by liquid closed in centrifuge tube on the other hand, preventing pollution.
Concrete, the Drainage pipe 11 of the present embodiment is circular tube structure, and arranges from large to small along discharge direction diameter, and minimum diameter is identical with the diameter of sealing column 32.Drainage pipe 11 is arranged to narrow circular tube structure, liquid can be made to discharge in order, greatly improve the purity of collecting liquid.And the diameter of sealing column 32 is identical with the minimum diameter of Drainage pipe 11, when sealing column 32 can be made to insert Drainage pipe 11, whole connection sealing No leakage, centrifugal effect when ensure that centrifugal.
In the present embodiment, cylinder 21 and the inwall of cap lid 4 are also provided with the screw thread of working in coordination, and cylinder 21 is covered tightly by threaded engagement by cap lid 4, plays the effect of sealing equally.
In the present embodiment, body 1 is also provided with scale, and this scale is arranged on the top of at the bottom of pipe 3, for the measurement of liquid volume.
Below use procedure of the present utility model is described:
First, open pipe lid 2, adding liquid in body 1, then lid upper tube cap 2 (now at the bottom of pipe 3 be fixedly connected with body 1), carries out centrifugal work; After centrifugal end, open the cap lid 4 above pipe lid 2, liquid-transfering gun is connected on the cylinder 21 of pipe lid 2, to open at the bottom of pipe 3 afterwards, start liquid-transfering gun and discharge opeing is carried out to the liquid in body 1, the liquid of separation is discharged in order from Drainage pipe 11, finally, until the liquid of required extraction is discharged, collect, complete extraction work.
Obviously, above-described embodiment of the present utility model is only used to clearly demonstrate the utility model example, and is not the restriction to embodiment of the present utility model.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All do within spirit of the present utility model and principle any amendment, equivalent to replace and improvement etc., within the protection domain that all should be included in the utility model claim.

Claims (9)

1. a fluid separation applications centrifuge tube, it is characterized in that, comprise body (1), be arranged on the pipe lid (2) that body (1) covers tightly by body (1) upper end, and at the bottom of the pipe being movably connected on body (1) lower end (3), described body (1) lower end is provided with Drainage pipe (11), when described centrifuge tube is centrifugal, described Drainage pipe (11) is placed in (3) at the bottom of pipe, and is sealed by (3) at the bottom of pipe; Described pipe lid (2) is provided with the cylinder (21) connecting liquid-transfering gun, described cylinder (21) is in hollow tubular structure, and be connected with body (1), described cylinder (21) is stamped cap lid (4).
2. fluid separation applications centrifuge tube according to claim 1, it is characterized in that, described body (1) lower end is provided with the taper tube wall (12) extending to Drainage pipe (11), upper end is provided with boss (13), and what described pipe lid (2) sealed is set on boss (13).
3. fluid separation applications centrifuge tube according to claim 2, it is characterized in that, described boss (13) outer wall and pipe lid (2) inwall are provided with the screw thread of working in coordination, screw tight pipe lid (2), boss (13) sealing covers tightly by described pipe lid (2).
4. fluid separation applications centrifuge tube according to claim 2, it is characterized in that, (3) upper end at the bottom of described pipe is provided with the conical depression (31) with taper tube wall (12) mating shapes, described conical depression (31) goes up and taper tube wall (12) is provided with the screw thread of working in coordination, screw (3) at the bottom of pipe, while the coated described taper tube wall (12) of conical depression (31), at the bottom of described pipe, Drainage pipe (11) seals by (3).
5. fluid separation applications centrifuge tube according to claim 4, it is characterized in that, at the bottom of described pipe, (3) inside is provided with sealing column (32), described sealing column (32) is fixedly installed on the bottom of (3) at the bottom of pipe, at the bottom of described pipe time (3) sealing Drainage pipe (11), described sealing column (32) is inserted described Drainage pipe (11) and Drainage pipe (11) sealing is filled.
6. fluid separation applications centrifuge tube according to claim 5, is characterized in that, described Drainage pipe (11) is circular tube structure, and along discharge direction diameter from large to small, and minimum diameter is identical with the diameter of sealing column (32).
7. fluid separation applications centrifuge tube according to claim 1, it is characterized in that, described cylinder (21) and cap lid (4) inwall are provided with the screw thread of working in coordination, and cylinder (21) is covered tightly by threaded engagement by described cap lid (4).
8. fluid separation applications centrifuge tube according to claim 1, is characterized in that, described body (1) is provided with scale.
9. according to the arbitrary described fluid separation applications centrifuge tube of claim 1-8, it is characterized in that, the interface place of described cylinder (21) bottom surface and Guan Gai (2) is provided with cruciform baffles agitated vessel (22), and described cruciform baffles agitated vessel (22) is connected to the inwall of cylinder (21) and one-body molded with it; (22) are placed with sterile cotton to described cruciform baffles agitated vessel, the inside of described sterile cotton filling disks cylinder (21).
CN201520243842.XU 2015-04-21 2015-04-21 A kind of fluid separation applications centrifuge tube Active CN204710574U (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106179552A (en) * 2016-07-06 2016-12-07 江苏科华医疗器械科技有限公司 A kind of integral type ultra-filtration centrifuge tube
CN106442091A (en) * 2016-10-31 2017-02-22 上海化工研究院 Device for enriching trace volatile organic matters in water-containing solid on basis of hot-gas blow-off methods
CN108485935A (en) * 2018-04-04 2018-09-04 柳州市妇幼保健院 A kind of sperm preferred embodiment and method
CN109762726A (en) * 2018-12-27 2019-05-17 临海市科晶生物科技有限公司 A kind of cell separation apparatus and its application method of simple and efficient

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106179552A (en) * 2016-07-06 2016-12-07 江苏科华医疗器械科技有限公司 A kind of integral type ultra-filtration centrifuge tube
CN106442091A (en) * 2016-10-31 2017-02-22 上海化工研究院 Device for enriching trace volatile organic matters in water-containing solid on basis of hot-gas blow-off methods
CN108485935A (en) * 2018-04-04 2018-09-04 柳州市妇幼保健院 A kind of sperm preferred embodiment and method
CN108485935B (en) * 2018-04-04 2023-07-04 柳州市妇幼保健院 Sperm optimizing device and method
CN109762726A (en) * 2018-12-27 2019-05-17 临海市科晶生物科技有限公司 A kind of cell separation apparatus and its application method of simple and efficient

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