CN205313537U - Circulation tumor cells collection device - Google Patents
Circulation tumor cells collection device Download PDFInfo
- Publication number
- CN205313537U CN205313537U CN201521004810.0U CN201521004810U CN205313537U CN 205313537 U CN205313537 U CN 205313537U CN 201521004810 U CN201521004810 U CN 201521004810U CN 205313537 U CN205313537 U CN 205313537U
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- filter tube
- filter
- connecting portion
- collection device
- chassis
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Abstract
The utility model belongs to the technical field of it is biological, concretely relates to circulation tumor cells collection device, including supplementary chimney filter, filter tube and collet, supplementary chimney filter includes the assisted parts that is cylindrical connecting portion and is connected with the one end of connecting portion, and the diameter of assisted parts is greater than the diameter of connecting portion, the filter tube is hollow column, and connecting portion can insert the one end of filter tube, and the inside holding of filter tube has a filter membrane, the collet asks the post to locate the one side on chassis including being cylindric chassis and cylindric support post, and the diameter on chassis is greater than the diameter that holds in the palm the post, and the support post can insert the other end of filter tube, is equipped with the hole of the fluid passage of being convenient for on the chassis. The utility model discloses a circulation tumor cells collection device can be big or small according to the cell, and through filtering direct enrichment epithelium nature tumor cells, this kind installs easy operation, and the universality is strong.
Description
Technical field
This utility model belongs to biological technical field, is specifically related to a kind of circulating tumor cell collection device.
Background technology
Approach according to Nasopharyngeal neoplasms, in blood or lymphatic vessel, the tumor cell of circulation is defined as circulating tumor cell (circulatingtumorcell, CTC), owing to circulating tumor cell content is considerably less and lacks effective Specific marker, the progress about CTC is slower. The method of existing enrichment CTC mainly has Percoll gradient centrifugation, immunomagnetic isolation method etc., and all kinds of corrupt practices creep in for it.
Utility model content
The purpose of this utility model is to provide a kind of circulating tumor cell collection device, it is possible to according to cell size, and by filtering directly enrichment epithelial tumour cell, this kind of device is simple to operate, and universality is strong.
For achieving the above object, this utility model be the technical scheme is that to include auxiliary chimney filter, filter tube and collet, and auxiliary chimney filter includes the connecting portion in column and the assisted parts being connected with one end of connecting portion, and the diameter of assisted parts is more than the diameter of connecting portion; Filter tube is the column of hollow, and connecting portion can be inserted into one end of filter tube, and the accommodated inside of filter tube has a filter membrane; Collet includes cylindrical chassis and columned holder post, and the side on chassis is located at by holder post, and the diameter on chassis is more than the diameter of holder post, and holder post can be inserted into the other end of filter tube, and chassis is provided with is easy to the hole that liquid passes through.
Preferably, the outer wall of described connecting portion is provided with external screw thread, and the inwall of filter tube one end is provided with female thread, and connecting portion is spirally connected by external screw thread and cooperating of female thread with filter tube.
Preferably, the inwall of the other end of described filter tube is provided with female thread, and the outer wall of holder post is provided with external screw thread, and filter tube is spirally connected by external screw thread and cooperating of female thread with holder post.
Preferably, the internal diameter of one end of described filter tube more than the internal diameter of the other end, filter tube be internally formed a ladder port, described filter membrane is placed in ladder port.
Preferably, described auxiliary chimney filter is funnel-form.
Preferably, one end extension of the opening of described assisted parts is provided with a lid, the opening part of the turnover sealing assisted parts of lid.
Preferably, the port away from the one end on chassis of described holder post is provided with crossbeam, and crossbeam is by the radially uniform setting of the port of three rod members edge holder posts.
Preferably, the aperture on described filter membrane is 5-10 μm, and the material of filter membrane is nylon or PC.
After adopting technique scheme, this utility model has the positive effect that:
After filter tube in this utility model and collet coordinate, holder post can hold the filter membrane in filter tube, prevent filter membrane from breakage occurring in centrifugal process, the circulating tumor cell needed is trapped within filter membrane, other cells are flowed out via the space between filter tube and holder post is centrifugal from the bottom on chassis by filter membrane, enrichment is convenient, and screening efficiency is high.
Accompanying drawing explanation
Fig. 1 is the sectional view of circulating tumor cell collection device of the present utility model;
Fig. 2 is the filter tube structural representation in this utility model;
Fig. 3 is the collet configuration schematic diagram in this utility model.
Wherein: 1, auxiliary chimney filter, 11, connecting portion, 12, assisted parts, 13, lid, 2, filter tube, 21, ladder port, 3, collet, 31, chassis, 311, hole, 32, holder post, 321, crossbeam.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, this utility model is described in further detail.
As Figure 1-3, for circulating tumor cell collection device of the present utility model, including auxiliary chimney filter 1, filter tube 2 and collet 3.
Auxiliary chimney filter 1 includes connecting portion 11 and assisted parts 12, connecting portion 11 and assisted parts 12 are all cylindrical, one end of connecting portion 11 is connected with one end of assisted parts 12, and the diameter of assisted parts 12 is more than the diameter of connecting portion 11, and the entirety of connecting portion 11 and assisted parts 12 is funnel-form. One end extension of assisted parts 12 opening is provided with a lid 13, the turnover opening part sealing up assisted parts 12 of lid 13. The outer wall of the other end of connecting portion 11 is provided with external screw thread.
The outside of filter tube 2 is column, inside is hollow, and the internal diameter of one end of filter tube 2 is more than the internal diameter of the other end, thus it is internally formed a ladder port 21, at ladder port 21 place's holding one filter membrane, aperture on filter membrane is 5-10 μm, and the material of filter membrane is nylon or PC (Merlon). The other end of connecting portion 11 can insert the inside of filter tube 2 from one end that the internal diameter of filter tube 2 is bigger, and arrive the top of filter membrane present position, the internal diameter of bigger one end of internal diameter of filter tube 2 is slightly larger than the external diameter of connecting portion 11, and the inwall of bigger one end of internal diameter of filter tube 2 is provided with female thread, by coordinating of the external screw thread on connecting portion 11 and described female thread, connecting portion 11 and filter tube 2 is made to be spirally connected.
Collet 3 includes chassis 31 and holder post 32, chassis 31 and holder post 32 are all cylindrical, and the diameter on chassis 31 is more than the diameter of holder post 32, the side on chassis 31 is located at by holder post 32, the upper and lower surface on chassis 31 is all provided with porose 311, it is simple to Cell sap passes through, and the entirety of chassis 31 and holder post 32 is T-shaped. The port away from the one end on chassis 31 of holder post 32 is provided with crossbeam 321, and crossbeam 321 is by the radially uniform setting of the port of three rod members edge holder posts 32, and crossbeam 321 plays the effect supporting filter membrane. Holder post 32 in place's can insert the inside of filter tube 2 from the less one end of the internal diameter of filter tube 2, and arrives the lower section of filter membrane location, and thus holder post 32 can hold filter membrane.The inwall of less one end of internal diameter of filter tube 2 is provided with female thread, and holder post 32 is provided with the external screw thread with screw-internal thread fit, is coordinated so that holder post 32 is spirally connected with filter tube 2 with externally threaded by female thread.
Circulating tumor cell collection device of the present utility model is to cooperate with what 50ml centrifuge tube used, and it can just be put into inside 50ml centrifuge tube, and the external diameter of circulating tumor cell collection device is only smaller than the internal diameter of 50ml centrifuge tube. First injecting blood from auxiliary chimney filter 1 during use, tumor cell to be enriched with is by membrane retention above filter membrane, and other cells pass through from filter membrane and flow out from the base apertures 311 on chassis 31 via the space between filter tube 2 and holder post 32. After centrifugal filtration, auxiliary chimney filter 1 is backed out, filter tube 2 is tipped upside down on the mouth of pipe of 5ml centrifuge tube, back out collet 3 again, with the liquid transfer gun head top of 1ml by bottom the filter membrane top in filter tube 2 to centrifuge tube, being taken off from centrifuge tube by filter tube 2, the filter membrane stayed can carry out follow-up operation again, carries out DNA extraction, cell analysis etc. as added extracting solution.
It will be apparent to those skilled in the art that can technical scheme as described above and design, make other various corresponding changes and deformation, and all these change and deformation all should belong within the protection domain of this utility model requirement.
Claims (8)
1. a circulating tumor cell collection device, it is characterized in that: include auxiliary chimney filter (1), filter tube (2) and collet (3), auxiliary chimney filter (1) includes the connecting portion (11) in column and the assisted parts (12) being connected with one end of connecting portion (11), and the diameter of assisted parts (12) is more than the diameter of connecting portion (11); The filter tube (2) column in hollow, connecting portion (11) can be inserted into one end of filter tube (2), and the accommodated inside of filter tube (2) has a filter membrane; Collet (3) includes cylindrical chassis (31) and columned holder post (32), the side of chassis (31) is located in holder post (32), the diameter on chassis (31) is more than the diameter of holder post (32), holder post (32) can be inserted into the other end of filter tube (2), and chassis (31) are provided with the hole (311) be easy to liquid and pass through.
2. circulating tumor cell collection device according to claim 1, it is characterized in that: the outer wall of connecting portion (11) is provided with external screw thread, the inwall of filter tube (2) one end is provided with female thread, and connecting portion (11) is spirally connected by external screw thread and cooperating of female thread with filter tube (2).
3. circulating tumor cell collection device according to claim 2, it is characterized in that: the inwall of the other end of filter tube (2) is provided with female thread, the outer wall of holder post (32) is provided with external screw thread, and filter tube (2) is spirally connected by external screw thread and cooperating of female thread with holder post (32).
4. circulating tumor cell collection device according to claim 3, it is characterized in that: the internal diameter of one end of filter tube (2) is more than the internal diameter of the other end, filter tube (2) be internally formed a ladder port (21), described filter membrane is placed in ladder port (21) place.
5. the circulating tumor cell collection device according to any one of claim 1-4, it is characterised in that: auxiliary chimney filter (1) is in funnel-form.
6. circulating tumor cell collection device according to claim 5, it is characterized in that: one end extension of the opening of assisted parts (12) is provided with a lid (13), the opening part of the turnover sealing assisted parts (12) of lid (13).
7. circulating tumor cell collection device according to claim 3, it is characterized in that: the port away from the one end of chassis (31) of holder post (32) is provided with crossbeam (321), crossbeam (321) is by the radially uniform setting of the port of three rod members edge holder post (32).
8. circulating tumor cell collection device according to claim 1, it is characterised in that: the aperture on described filter membrane is 5-10 μm, and the material of filter membrane is nylon or PC.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521004810.0U CN205313537U (en) | 2015-12-07 | 2015-12-07 | Circulation tumor cells collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521004810.0U CN205313537U (en) | 2015-12-07 | 2015-12-07 | Circulation tumor cells collection device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205313537U true CN205313537U (en) | 2016-06-15 |
Family
ID=56315048
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201521004810.0U Active CN205313537U (en) | 2015-12-07 | 2015-12-07 | Circulation tumor cells collection device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205313537U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112577793A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Double-layer membrane filter cup and application thereof |
-
2015
- 2015-12-07 CN CN201521004810.0U patent/CN205313537U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112577793A (en) * | 2019-09-29 | 2021-03-30 | 广东体必康生物科技有限公司 | Double-layer membrane filter cup and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200715 Address after: 213000 Jiangsu city of Changzhou province Wujin Jincheng Road Economic Development Zone No. 18 Patentee after: Fulong Medical Devices Group Co.,Ltd. Address before: 213149 Jiangsu province Changzhou Jincheng Economic Development Zone Wujin Road No. 18 Patentee before: CHANGZHOU FULONG BIOLOGICAL Co.,Ltd. |
|
| TR01 | Transfer of patent right |