CN202359118U - Gradient separation device of cast-off cells - Google Patents

Gradient separation device of cast-off cells Download PDF

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Publication number
CN202359118U
CN202359118U CN 201120491450 CN201120491450U CN202359118U CN 202359118 U CN202359118 U CN 202359118U CN 201120491450 CN201120491450 CN 201120491450 CN 201120491450 U CN201120491450 U CN 201120491450U CN 202359118 U CN202359118 U CN 202359118U
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China
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dropping
minim pipette
cells
cast
pipette
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Expired - Fee Related
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CN 201120491450
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Chinese (zh)
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石国清
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/10—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by centrifugation ; Cyclones

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Centrifugal Separators (AREA)

Abstract

The utility model relates to a gradient separation device of cast-off cells, comprising a centrifugal pipe (1) and a dropping pipette (2). The gradient separation device is characterized in that the dropping pipette (2) is erected in the centrifugal pipe (1), and the side wall of the dropping pipette (2) is provided with a dropping hole (3) for a dropping agent to flow out and a bracket (5) capable of being erected in the centrifugal pipe (1); and a gap (4) for the dropping agent to flow down along the inner wall of the centrifugal pipe (1) is arranged between the outer side wall of the dropping pipette (2) and an end part crossed with the bottom part of the dropping pipette as well as between the outer side wall of the dropping pipette (2) and the inner wall of the centrifugal pipe (1). The gradient separation device of the cast-off cells has the beneficial effects that the dropping pipette is erected in the centrifugal pipe, the side wall of the dropping pipette is provided with the dropping hole for the dropping agent to flow out, and the gap for the dropping agent to flow down along the inner wall of the centrifugal pipe is arranged among the outer side wall of the dropping pipette and the end part crossed with the bottom part of the dropping pipette as well as between the outer side wall of the dropping pipette (2) and the inner wall of the centrifugal pipe, so that the liquid transferring work can be simply and conveniently finished; and the gradient separation device can be used in a large-batched way so that the working efficiency is improved and the cost is reduced.

Description

A kind of cast-off cells gradient separations device
Technical field
The utility model relates to a kind of device that medical profession and pathocytology are used to separate various cells that is used for.
Background technology
At present, in medical treatment and pathocytology industry,, when cast-off cells are detected, often need liquid to be checked be carried out spinning earlier for extracting required cell.Earlier add gradient centrifugation liquid in the conical centrifuge tube in advance; Draw the cell suspension of aequum then with disposable pipet; Suction pipe is placed the conical centrifuge tube tube wall, slowly add cell suspension, can not make cell suspension and gradient centrifugation liquid-phase mixing along tube wall.Caused under loaded down with trivial details, the working efficiency of operation like this and be difficult for detecting in enormous quantities.
The utility model content
The purpose of the utility model provides a kind of high efficiency, simple to operate and a kind of cast-off cells gradient separations device that can detect in enormous quantities.
In order to accomplish above-mentioned purpose, the technical scheme that the utility model adopts is:
A kind of cast-off cells gradient separations device; Comprise centrifuge tube and minim pipette; Described minim pipette is erected in the centrifuge tube; On the minim pipette sidewall, be provided with and supply detect the effusive dripping hole of test solution, leave between the end that the outer side wall of described minim pipette and its bottom cross and the inwall of centrifuge tube and supply the detection test solution along gap that the centrifuge tube inwall flows down.
Described dripping hole is arranged on the sidewall of minim pipette and the end that its bottom crosses, and protruding arch is formed on the bottom of minim pipette.
The outer side wall lower end of described minim pipette and the section that forms that crosses bottom it are isosceles trapezoid.
Described gap is 0.1-5mm.
Described gap is 1mm.
The beneficial effect of the utility model is: be employed in and set up minim pipette in the centrifuge tube; Sidewall setting at minim pipette makes the effusive dripping hole of detection test solution; Leave between end that the outer side wall of minim pipette and bottom cross and the centrifuge tube inwall and supply to detect test solution along gap that the centrifuge tube inwall flows down; Can accomplish the liquid work that moves simply and easily, and can use in enormous quantities, improve working efficiency.
Description of drawings
Fig. 1 is the diagrammatic cross-section of the utility model.
Fig. 2 is the diagrammatic cross-section of minim pipette among Fig. 1.
Fig. 3 is the diagrammatic cross-section of second kind of structure of minim pipette among Fig. 1.
Fig. 4 is the diagrammatic cross-section of the third structure of minim pipette among Fig. 1.
Among the figure, 1, centrifuge tube, 2, minim pipette, 3, dripping hole, 4, the gap, 5, carriage.
Embodiment
The utility model is a kind of cast-off cells gradient separations device; Be employed in and set up minim pipette in the centrifuge tube; Sidewall setting at minim pipette makes the effusive dripping hole of detection test solution, leaves between end that the outer side wall of minim pipette and bottom cross and the centrifuge tube inwall to supply to detect test solution along the gap that the centrifuge tube inwall flows down, and can accomplish simply and easily and detect test; And can use in enormous quantities, improve working efficiency.
Below in conjunction with accompanying drawing the utility model is further specified.
Specific embodiment 1; Like Fig. 1, shown in Figure 2; A kind of cast-off cells gradient separations device comprises centrifuge tube 1 and the minim pipette 2 that is erected in the centrifuge tube 1, on minim pipette 2 sidewalls, is provided with to supply to detect the effusive dripping hole 3 of test solution and can be erected at the carriage 5 in the centrifuge tube 1; Leave between the end that the outer side wall of minim pipette 2 and its bottom cross and the inwall of centrifuge tube 1 and supply to detect test solution along the gap 4 that centrifuge tube 1 inwall flows down, gap 4 is 0.1mm.
In the time need carrying out the dropping liquid detection to the liquid to be checked after separating; Elder generation is erected at carriage 5 on the centrifuge tube 1 and makes minim pipette 2 be positioned at centrifuge tube 1; To detect test solution and inject in the minim pipette 2, the detection test solution is from dripping hole 3 outflows and in gap 4, form water droplet, because gap 4 is 0.1mm; Detect test solution and can flow down entering liquid to be checked, accomplish and detect test along centrifuge tube 1 inwall.
Specific embodiment 2, as shown in Figure 3, as improvement to the utility model; Gap 4 is 1mm, and protruding arch is formed on the bottom of minim pipette 2, and dripping hole 3 is arranged on the sidewall of minim pipette 2 and the end that its bottom crosses; Detect the end formation water droplet that test solution flows out and crosses in sidewall and its bottom of minim pipette 2 from dripping hole 3 like this; Because gap 4 is 1mm, detects test solution and can flow down entering liquid completion to be checked detection test, because the arch of convexity is formed on the bottom of minim pipette 2 along the inwall of centrifuge tube 1; Detecting test solution can flow out from dripping hole 3 fully, can not flow down residual liquid in the minim pipette 2.
Specific embodiment 3, as shown in Figure 4, as improvement to the utility model; Gap 4 is 5mm, and the outer side wall lower end of minim pipette 2 and its bottom section that forms that crosses is an isosceles trapezoid, detects test solution like this and flows out and flow down along the outer side wall of minim pipette 2 from dripping hole 3;, the detection test solution in gap 4, forms water droplet when flowing to the bottom end of minim pipette 2; Because gap 4 is 5mm, detect test solution and can flow down entering liquid to be checked along the inwall of centrifuge tube 1, accomplish and detect test.The utility model is employed in and sets up minim pipette in the centrifuge tube; Sidewall setting at minim pipette makes the effusive dripping hole of detection drops; Leave between end that the outer side wall of minim pipette and bottom cross and the centrifuge tube inwall and supply drops along gap that the centrifuge tube inwall flows down; Can accomplish simply and easily and detect test, and can use in enormous quantities, improve working efficiency.

Claims (6)

1. cast-off cells gradient separations device; Comprise centrifuge tube (1) and minim pipette (2); It is characterized in that: described minim pipette (2) is erected in the centrifuge tube (1); On minim pipette (2) sidewall, be provided with and supply detect the effusive dripping hole of test solution (3), leave confession detection test solution between the end that the outer side wall of described minim pipette (2) and its bottom cross and the inwall of centrifuge tube (1) along gap (4) that centrifuge tube (1) inwall flows down.
2. a kind of cast-off cells gradient separations device according to claim 1 is characterized in that: described dripping hole (3) is arranged on the sidewall of minim pipette (2) and the end that its bottom crosses, and protruding arch is formed on the bottom of minim pipette (2).
3. a kind of cast-off cells gradient separations device according to claim 1 is characterized in that: the outer side wall lower end of described minim pipette (2) and the section that forms that crosses bottom it are isosceles trapezoid.
4. a kind of cast-off cells gradient separations device according to claim 1 is characterized in that: described minim pipette (2) is provided with the carriage (5) that can be erected in the centrifuge tube (1).
5. a kind of cast-off cells gradient separations device according to claim 1 is characterized in that: described gap (4) are 0.1-5mm.
6. a kind of cast-off cells gradient separations device according to claim 1 is characterized in that: described gap (4) are 1mm.
CN 201120491450 2011-12-01 2011-12-01 Gradient separation device of cast-off cells Expired - Fee Related CN202359118U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201120491450 CN202359118U (en) 2011-12-01 2011-12-01 Gradient separation device of cast-off cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201120491450 CN202359118U (en) 2011-12-01 2011-12-01 Gradient separation device of cast-off cells

Publications (1)

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CN202359118U true CN202359118U (en) 2012-08-01

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103849562A (en) * 2012-12-05 2014-06-11 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN105132277A (en) * 2015-09-29 2015-12-09 杭州科鼎生物科技有限公司 Novel lymphocyte separation tube

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103849562A (en) * 2012-12-05 2014-06-11 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN103849562B (en) * 2012-12-05 2015-06-10 北京东方华辉生物医药科技有限公司 Sleeve for biological sample in-vitro purification treatment
CN105132277A (en) * 2015-09-29 2015-12-09 杭州科鼎生物科技有限公司 Novel lymphocyte separation tube
CN105132277B (en) * 2015-09-29 2018-03-09 杭州科鼎生物科技有限公司 A kind of new separation of lymphocytes pipe

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20120801

Termination date: 20141201

EXPY Termination of patent right or utility model