CN207699577U - Filter absorption type nucleic acid rapid extraction device - Google Patents
Filter absorption type nucleic acid rapid extraction device Download PDFInfo
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- CN207699577U CN207699577U CN201721649121.4U CN201721649121U CN207699577U CN 207699577 U CN207699577 U CN 207699577U CN 201721649121 U CN201721649121 U CN 201721649121U CN 207699577 U CN207699577 U CN 207699577U
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- adsorption column
- nucleic acid
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- extraction device
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Abstract
The utility model discloses filtering absorption type nucleic acid rapid extraction devices, including syringe, filter, filter membrane, adsorption column upper part, adsorbed film, filter plate, adsorption column lower part and suction nozzle, wherein, adsorption column upper part includes upper portion pipe body and lower pipe body, and lower pipe body outer layer is threaded；Filter is divided into circular two parts, upper part up and down and is connected with injector head, and lower part is connected with adsorption column upper part, and filter membrane is placed between top and the bottom and is sealed with ultrasonic fusing.The utility model is intended to provide a kind of filtering absorption type nucleic acid rapid extraction device, the nucleic acid in sample can directly and quickly be extracted, its extraction process is without using instrument and equipments such as centrifuges, the plenty of time is saved, many operating procedures are simplified, are suitable for scene and some application of special occasions, while eliminating plastic chuck ring, residual liquid can be effectively avoided to improve the accuracy of experiment to the influence of amplification environment.
Description
Technical field
The utility model is related to technical field of molecular biology, and in particular to filtering absorption type nucleic acid rapid extraction device.
Background technology
Nucleic acid is extracted from various biological samples, is had become in technical field of molecular biology most basic and the most frequently used
Technology.The method of extraction nucleic acid is mainly the following at present：
1. organic extraction method
Basic principle：Phenol makes albuminous degeneration, SDS lytic cell films, Proteinase K and EDTA digestible proteins or polypeptide or small
Peptide, nucleoprotein denaturation degradation make DNA separate outs from nucleoprotein.It is soluble easily in water using DNA, the characteristic insoluble in organic solvent
Extract DNA.
2. chelating resin method
Basic principle：By taking master chelating resin chelex-100 to be used as an example, contain pairs of iminodiacetic acid
Salt ion has very high affinity and chelation to high volence metal ion.It can make in low ionic strength, alkalinity and under boiling
Cell membrane lysis, and make albuminous degeneration, DNA is free.
3. inorganic extraction method
Basic principle：Using NaCI or sodium acetate precipitation albumen, through centrifugation, extraction DNA is precipitated with absolute ethyl alcohol.
4. differential lysis extraction method
Basic principle：Cell membrane using certain biological samples is easily broken, and the cell of other biological samples is not easy
Broken feature is first removed easily broken cell using conventional method, then is not easy smudge cells with special reagent cracking, then is carried
Take DNA therein.
5. phosphate buffer method
Basic principle：After cell digests in the solution containing non-ionic octoxynol detergent and Proteinase K, high-temperature heating makes protein
And Proteinase K inactivation, centrifugation protein isolate matter extract DNA from centrifuged supernatant.
6. formamide depolymerization
Basic principle：The compound of formamide cleavable DNA and protein, and the protein of changeability precipitation release, but
It does not make significant difference to the activity of Proteinase K.DNA and protein in chromatin are cracked with highly difficult formamide, then uses collodion
Bag carries out fully dialysis removal Proteinase K and organic solvent.This method be suitable for build high power capacity carrier genome dna library and
Carry out the pulsed-field gel electrophoresis analysis of large fragment.
7. glass bar winding method
Basic principle：DNA can be deposited in the interface of cell pyrolysis liquid and ethyl alcohol, with the glass bar of buckle by large fragment
DNA in absolute ethyl alcohol from being transferred in the TE liquid of PH8.0.This method is suitable for structure gene library, and PCR amplification is suitable for simultaneously
DNA is extracted from different cell or tissue samples.
8. the method based on silica adsorbing and extracting nucleic acid
Basic principle：The characteristic of nucleic acid is adsorbed using silica, organism through guanidinium isothiocyanate, roton X-100's
Nucleic acid is combined with silica after Tris-HCl-EDTA processing, then passes through the Tris-HCl buffer solutions and 70% containing guanidinium isothiocyanate
Ethyl alcohol and acetone wash respectively, are finally eluted with TE, obtain nucleic acid.
9. glass fibre membrane absorption method
Basic principle：Glass fibre membrane has the characteristics that be combined with nucleic acid invertibity, and the DNA in organism lysate is promoting
It is combined with glass fibre membrane under the action of adsorption liquid, it is miscellaneous with the albumen and other adsorbed on protein liquid removal removal glass fibre membrane
Matter, then clean the albumen adsorbed on glass fibre membrane and other impurities again with cleaning solution, finally with eluent by glass fibre
The Nucleic Acid Elution adsorbed on film gets off.
10. paramagnetic particle method
Basic principle：Using the strong protein denaturant such as guanidine thiocyanate, destruction cell membrane and nuclear membrane albumen, released dna, and
Make nuclease-dead；Then magnetic bead is added by the chemical groups on surface and DNA specific adsorptions, and the impurity such as protein not by
It adsorbs and stays in appearance at night；Then under the influence of a magnetic field, magnetic-particle and liquid separate, and recycle particle；Finally pure water is used again
Or the DNA of TE elution absorption, the dissociation of DNA and magnetic bead are carried out in lysate, and DNA is dissolved out again.
11. pellosil adsorption column extraction method
Basic principle：Silicon moulds adsorption column method is released come selective absorption lytic cell using special Silicon moulds adsorption column
The DNA put, using the simple programs such as washing and elution, so that it may to obtain high-purity DNA.
At present in the world in terms of nucleic acid extraction, some methods complicated for operation and cumbersome are gradually eliminated.Glass fibre
Film absorption method and pellosil adsorption column extraction method have it is easy to operate, be easy to get high-purity DNA and RNA, adjusting can be passed through
The thickness of adsorbed film come adapt to extract different amounts of nucleic acid requirement and it is of low cost the advantages that, therefore be widely used to various
In nucleic acid detection kit.
As adsorption column or centrifugal column be all to be packed into adsorbed film from the top of column, then plastic chuck ring is used to compress suction
Membrane makes the liquid flowed through not leak.Since plastic chuck ring is there are dead angle, it is easy to remain the liquid of column, gene is caused to expand
The change for increasing environment, influences experimental result.
Utility model content
The purpose of this utility model is to provide a kind of filtering absorption type nucleic acid rapid extraction devices, based on most normal in the world
The glass fibre membrane absorption method and pellosil adsorption column extraction method used, the nucleic acid in lysed sample pass through the present apparatus, Ke Yizhi
The nucleic acid rapidly extracted in sample is connect, extraction process has been saved the plenty of time without using instrument and equipments such as centrifuges, letter
Many operating procedures are changed, have been suitable for scene and some application of special occasions, while eliminating plastic chuck ring, can effectively avoid
Influence of the residual liquid to amplification environment, improves the accuracy of experiment.
To achieve the goals above, the utility model uses following technical scheme：
Filter absorption type nucleic acid rapid extraction device, including syringe, filter, filter membrane, adsorption column upper part, suction
Membrane, filter plate, adsorption column lower part and suction nozzle, wherein
The adsorption column upper part includes upper portion pipe body and lower pipe body, and lower pipe body outer layer is threaded；
The filter is divided into circular two parts, upper part up and down and is connected with injector head, lower part and adsorption column
Upper part is connected, and filter membrane is placed between top and the bottom and is sealed with ultrasonic fusing；
The lower pipe body of the adsorption column lower part is threadedly coupled with adsorption column upper part；
Adsorbed film and filter plate are placed between the adsorption column upper part and adsorption column lower part, adsorbed film is placed in filter
Above plate.
Further, the capacity of the syringe is 1-100ml, and material is plastics or glass.
Further, the filter material is plastics, a diameter of 10-50mm.
Further, the material of the filter membrane is composite fibre film, cellulose acetate film, nitrocellulose membrane, nylon membrane, gathers
One kind in ether sulfone film or glass fibre membrane, thickness 0.1mm-10mm, aperture 0.1um-10um.
Further, the upper portion pipe body internal diameter of the adsorption column upper part is 5mm, and lower pipe body internal diameter is 7mm;
Further, the top end diameter of the suction nozzle is 5mm.
Further, the material of the adsorbed film is selected as glass fibre membrane or pellosil, thickness 0.1mm-10mm.
Further, the material of the filter plate is in plastic multi hole plate, nylon wire, metal mesh or Multiple Shape hollow plastic rack
One kind.
Further, the adsorption column upper part and adsorption column lower part inner wall are coated with silicone oil.
Further, the tube body of the adsorption column lower part is divided into two layers inside and outside, and tube body internal layer center is equipped with one
Funnel-shaped hole extends to suction nozzle, and internal layer pipe diameter is less than the lower pipe body internal diameter of adsorption column upper part.
Compared with prior art, the utility model has the advantage that：
Utility model device can directly and quickly extract the nucleic acid in sample, and extraction process is without using centrifuge
Equal instrument and equipments, have saved the plenty of time, have simplified many operating procedures, are suitable for scene and some application of special occasions, together
When eliminate plastic chuck ring, effectively prevent residual liquid to expand environment influence, improve the accuracy of experiment.
Description of the drawings
Fig. 1 is the schematic diagram of the utility model.
In figure：1, syringe；2, filter；3, filter membrane；4, adsorption column upper part；5, adsorbed film；6, filter plate；7, it inhales
Attached column lower part；8, suction nozzle；401, upper portion pipe body；402, lower pipe body.
Specific implementation mode
The utility model is further described with reference to specific embodiment, but examples are merely exemplary, it is not right
The scope of the utility model constitutes any restrictions.It will be understood by those skilled in the art that without departing from the utility model
Can the details and form of technical solutions of the utility model be modified or be replaced under spirit and scope, but these are changed and replace
It changes and each falls in the scope of protection of the utility model.
As shown in Figure 1, filtering absorption type nucleic acid rapid extraction device, including syringe 1, filter 2, filter membrane 3, absorption
Column upper part 4, adsorbed film 5, filter plate 6, adsorption column lower part 7 and suction nozzle 8, wherein the adsorption column upper part includes
Upper portion pipe body 401 and lower pipe body 402,402 outer layer of lower pipe body are threaded；The filter 2 is divided into two above and below circular
Part, upper part are connected with 1 head of syringe, and lower part is connected with adsorption column upper part 4, is placed between top and the bottom
Filter membrane 3 is simultaneously sealed with ultrasonic fusing；402 screw thread of lower pipe body of the adsorption column lower part 7 and adsorption column upper part 4
Connection specifically uses anti-retrogression screw thread, so that the adsorbed film of fastening is not loosened caused by the reasons such as vibrations, ensured column liquid not
It can leak；Adsorbed film 5 and filter plate 6 are placed between the adsorption column upper part 4 and adsorption column lower part 7, adsorbed film 5 is set
Above filter plate 6, filter plate 6 plays the role of supporting adsorbed film 5；The capacity of the syringe 1 is 1-100ml, and material is plastics
Or glass；2 material of the filter is plastics, a diameter of 10-50mm；The material of the filter membrane 3 is composite fibre film, acetic acid
One kind in tunica fibrosa, nitrocellulose membrane, nylon membrane, poly (ether sulfone) film or glass fibre membrane, thickness 0.1mm-10mm, aperture is
0.1um-10um；401 internal diameter of upper portion pipe body of the adsorption column upper part 4 is 5mm, and the top end diameter of suction nozzle 8 is 5mm, is made
It is flowed out under conditions of uniform pressure by the liquid of adsorption column upper part 4 and adsorption column lower part 7, effectively avoids liquid
Residual in cylinder, 402 internal diameter of lower pipe body are 7mm；The material of the adsorbed film 5 is glass fibre membrane or pellosil, thickness
Degree is 0.1mm-10mm；The material of the filter plate 6 is in plastic multi hole plate, nylon wire, metal mesh or Multiple Shape hollow plastic rack
One kind；The adsorption column upper part 4 and 7 inner wall of adsorption column lower part are coated with silicone oil, reduce the residual of column liquid；Institute
The tube body for stating the adsorption column lower part 7 is divided into inside and outside two layers, and tube body internal layer center extends to suction nozzle equipped with a funnel-shaped hole
8, internal layer pipe diameter is less than the internal diameter of the lower pipe body 401 of adsorption column upper part 4.
Specific operation process：The sample handled well has been cracked with the sucking of syringe 1, sample in syringe 1 has slowly been pushed away
Enter filter 2, the sample filtered out from filter 2 syringe 1 pushing under continue to flow into adsorption column upper part 4, adsorbed film 5,
Filter plate 6 and adsorption column lower part 7 are flowed out through suction nozzle 8, and the liquid of outflow can discard, and the nucleic acid in sample is adsorbed at this time
On adsorbed film 5.
It takes a new syringe to be closely coupled to adsorption column upper part 4, draws cleaning solution with syringe, then release
Cleaning solution discards cleaning solution.Eluent is drawn with syringe again, syringe is aspirated back and forth makes eluent iterate through adsorbed film
5 for several times, finally releases eluent from suction nozzle 8, collects eluent for nucleic acid amplification.
Claims (10)
1. filtering absorption type nucleic acid rapid extraction device, which is characterized in that including on syringe, filter, filter membrane, adsorption column
Square component, adsorbed film, filter plate, adsorption column lower part and suction nozzle, wherein
The adsorption column upper part includes upper portion pipe body and lower pipe body, and lower pipe body outer layer is threaded；
The filter is divided into circular two parts, upper part up and down and is connected with injector head, lower part and adsorption column top
Component is connected, and filter membrane is placed between top and the bottom and is sealed with ultrasonic fusing；
The lower pipe body of the adsorption column lower part is threadedly coupled with adsorption column upper part；
It is placed with adsorbed film and filter plate between the adsorption column upper part and adsorption column lower part, adsorbed film is placed on filter plate
Face.
2. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the appearance of the syringe
Amount is 1-100ml, and material is plastics or glass.
3. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the filter material
For plastics, a diameter of 10-50mm.
4. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the material of the filter membrane
Material is one kind in composite fibre film, cellulose acetate film, nitrocellulose membrane, nylon membrane, poly (ether sulfone) film or glass fibre membrane, thickness
For 0.1mm-10mm, aperture 0.1um-10um.
5. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that above the adsorption column
The upper portion pipe body internal diameter of component is 5mm, and lower pipe body internal diameter is 7mm.
6. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the top of the suction nozzle
A diameter of 5mm.
7. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the material of the adsorbed film
Material is selected as glass fibre membrane or pellosil, thickness 0.1mm-10mm.
8. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the material of the filter plate
For one kind in plastic multi hole plate, nylon wire, metal mesh or Multiple Shape hollow plastic rack.
9. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that above the adsorption column
Component and adsorption column lower part inner wall are coated with silicone oil.
10. filtering absorption type nucleic acid rapid extraction device according to claim 1, which is characterized in that the absorption
The tube body of column lower part is divided into two layers inside and outside, and tube body internal layer center extends to suction nozzle equipped with a funnel-shaped hole, and internal layer tube body is straight
Diameter is less than the lower pipe body internal diameter of adsorption column upper part.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721649121.4U CN207699577U (en) | 2017-12-01 | 2017-12-01 | Filter absorption type nucleic acid rapid extraction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721649121.4U CN207699577U (en) | 2017-12-01 | 2017-12-01 | Filter absorption type nucleic acid rapid extraction device |
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| Publication Number | Publication Date |
|---|---|
| CN207699577U true CN207699577U (en) | 2018-08-07 |
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ID=63024563
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721649121.4U Expired - Fee Related CN207699577U (en) | 2017-12-01 | 2017-12-01 | Filter absorption type nucleic acid rapid extraction device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111944919A (en) * | 2020-07-31 | 2020-11-17 | 广东省农业科学院果树研究所 | Visual detection technology system for banana vascular wilt germ tropical No.4 microspecies capable of being operated in field at normal temperature |
| WO2021196508A1 (en) * | 2020-04-01 | 2021-10-07 | 宁波艾捷康宁生物科技有限公司 | Biological sample pretreatment reagent integrated adding method and device |
-
2017
- 2017-12-01 CN CN201721649121.4U patent/CN207699577U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021196508A1 (en) * | 2020-04-01 | 2021-10-07 | 宁波艾捷康宁生物科技有限公司 | Biological sample pretreatment reagent integrated adding method and device |
| CN111944919A (en) * | 2020-07-31 | 2020-11-17 | 广东省农业科学院果树研究所 | Visual detection technology system for banana vascular wilt germ tropical No.4 microspecies capable of being operated in field at normal temperature |
| CN111944919B (en) * | 2020-07-31 | 2023-05-26 | 广东省农业科学院果树研究所 | Banana fusarium wilt tropical No.4 small species visual detection technology system capable of being operated in field and at normal temperature |
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180807 Termination date: 20181201 |