CN206308344U - A kind of purification system for nucleic acid extraction - Google Patents
A kind of purification system for nucleic acid extraction Download PDFInfo
- Publication number
- CN206308344U CN206308344U CN201621423079.XU CN201621423079U CN206308344U CN 206308344 U CN206308344 U CN 206308344U CN 201621423079 U CN201621423079 U CN 201621423079U CN 206308344 U CN206308344 U CN 206308344U
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- China
- Prior art keywords
- negative pressure
- pressure box
- purification system
- valve
- nucleic acid
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- 238000000605 extraction Methods 0.000 title claims abstract description 41
- 238000000746 purification Methods 0.000 title claims abstract description 23
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 21
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 21
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 21
- 238000003828 vacuum filtration Methods 0.000 claims abstract description 18
- 238000010079 rubber tapping Methods 0.000 claims abstract description 15
- 229920001903 high density polyethylene Polymers 0.000 claims description 6
- 239000004700 high-density polyethylene Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- -1 polypropylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 239000007788 liquid Substances 0.000 abstract description 10
- 230000004907 flux Effects 0.000 abstract description 4
- 238000011160 research Methods 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 15
- 229920000297 Rayon Polymers 0.000 description 6
- 239000012528 membrane Substances 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 229960000583 acetic acid Drugs 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 235000011056 potassium acetate Nutrition 0.000 description 1
- 238000009609 prenatal screening Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Saccharide Compounds (AREA)
Abstract
The utility model is related to a kind of purification system for nucleic acid extraction, including vacuum filtration pump and negative pressure device, the negative pressure device includes negative pressure box, tapping valve, extraction valve and female Luer, the vacuum filtration pump connects extraction valve, the tapping valve, extraction valve are arranged on negative pressure box both sides, and the female Luer is arranged on the upper surface of negative pressure box；The vacuum filtration pump is used to coordinate tapping valve and extraction valve to extract negative pressure box internal gas；The flux of negative pressure box of the present utility model is big, conveniently topples over waste liquid, be placed on it is highly stable on experimental bench or workbench, it is simple to operate, it is not necessary to which that bottle,suction can reach and be directly connected to vacuum filtration pump, install and its simple, can be received by vast scientific research clients；In addition, the apparatus cost is low, other negative pressure device prices than commercial type are low, there is preferable market prospects.
Description
Technical field
The utility model is related to the extraction of biological sample and purification art, and in particular to a kind of purifying for nucleic acid extraction
System.
Background technology
With the development of biotechnology, the detection based on gene aspect is more and more universal, such as：Prenatal Screening, medical diagnosis on disease,
The aspects such as personalized medicine, major medical institutions, school and R＆D institution all tend to high degree of automation, the sample that flux is high
Processing scheme, traditional DNA extraction method fewer and feweri people use, and now widely used method has：Paramagnetic particle method, centrifugal column
The methods such as method, phenol chloroform method, boiling method, alkaline lysis, wherein with paramagnetic particle method and centrifugal column method use the most frequently, its
It is more that middle centrifugal column method is applied to scientific research clients.
It is conventional to have two methods for the technology that centrifugal column method extracts nucleic acid：Centrifuge method, negative pressure ventilation are separated
Method.Centrifugal process is based on based on the centrifuge of high speed, and sleeve pipe is coordinated by using centrifugal column, is repeatedly rushed from centrifuge and taken out
In the rotor of insertion centrifuge, the nucleic acid that collection is afforded.
In centrifugal method, the slightly many users of sample will feel that workload is big, centrifuge from 0~12000 rpm,
The slow centrifuge of lifting speed is more time-consuming.
Utility model content
In view of this, it is necessary to for problems of the prior art, there is provided a kind of purifying system for nucleic acid extraction
System, centrifuge is replaced using negative pressure device, saves more time and tedious work, can continuously isolate and purify multiple samples,
Quantization operation, and simple structure, it is easy to operate.
To achieve these goals, the technical solution of the utility model is as follows：
A kind of purification system for nucleic acid extraction, including vacuum filtration pump and negative pressure device, the negative pressure device include
Negative pressure box, tapping valve, extraction valve and female Luer, the vacuum filtration pump connect extraction valve, and the tapping valve, extraction valve are set
In negative pressure box both sides, the female Luer is arranged on the upper surface of negative pressure box；The vacuum filtration pump be used for coordinate tapping valve and
Extraction valve extracts negative pressure box internal gas.
Further, the negative pressure box is by one or more in high density polyethylene (HDPE) material, polypropylene, polystyrene
Combine the negative pressure box for constituting.
Further, the sheet metal thickness of the negative pressure box is 1.0cm~2.0cm.
Further, a length of 25cm~35cm of the negative pressure box, a width of 12.5cm~14.5cm, a height of 9.5cm~
10.5cm。
Further, spacing of the female Luer in negative pressure box upper surface is 1.5cm~3.0cm, and quantity is 12~48
It is individual.
Further, the position of the extraction valve is located at the upper end 0.5cm~1cm of negative pressure box side, the tapping valve
Position be located at extraction valve opposite side lower end 0.5cm~1cm at.
Further, the purification system also includes centrifugal column, and centrifugal column lower end can match female Luer, and volume is
1.5ml~50ml, the thickness of Silicon moulds is 1mm.
Further, the purification system includes three negative pressure devices, and three negative pressure devices are connected with vacuum filtration pump.
Compared with prior art, the flux of negative pressure box of the present utility model is big, conveniently topples over waste liquid, be placed on experimental bench or
It is highly stable on workbench, it is simple to operate, it is not necessary to which that bottle,suction can reach and be directly connected to vacuum filtration pump, install and its letter
It is single, can be received by vast scientific research clients；In addition, the apparatus cost is low, other negative pressure device prices than commercial type are low,
There are preferable market prospects.
Brief description of the drawings
Fig. 1 is a kind of structural representation of purification system for nucleic acid extraction of the present utility model.
Fig. 2 is the top view of the negative pressure device of purification system of the present utility model.
Specific embodiment
A kind of purification system for nucleic acid extraction described in the utility model is made with reference to the accompanying drawings and examples into
One step explanation.
The following is a kind of most preferred embodiment of purification system for nucleic acid extraction described in the utility model, not therefore
Limit protection domain of the present utility model.
Fig. 1 illustrates a kind of purification system for nucleic acid extraction, including vacuum filtration pump 1 and negative pressure device, described negative
Pressure device includes negative pressure box 5, tapping valve 3, extraction valve 2 and female Luer 4, and the vacuum filtration pump 1 connects extraction valve 2, described
Tapping valve 3, extraction valve 2 are arranged on the both sides of negative pressure box 5, and the female Luer 4 is arranged on the upper surface of negative pressure box.
Fig. 2 for negative pressure device top view, negative pressure device it is a length of：25cm~35cm, a width of 12.5cm~14.5cm are high
It is 9.5cm~10.5cm, the material for being used is one or more in high density polyethylene (HDPE) material, polypropylene, polystyrene
Combination, the thickness 1.0cm~2.0cm of the sheet material for being used.
The spacing of the female Luer 4 on negative pressure device surface is 1.5cm~3.0cm, the quantity of surface female Luer 4 for 12~
48.
The position of the extraction valve 2 of negative pressure device is located at the upper end 0.5cm~1cm of the side of negative pressure box 5, and tapping valve 3 is located at
Lower end 0.5cm~the 1cm of the relative side of extraction valve 2.
The characteristics of centrifugal column is that lower end can match female Luer 4, and volume is adapted to the centrifugal column of 1.5ml~50ml centrifuge tubes,
The thickness of pellosil is 1mm.
External vacuum filtration pump 1, three main bodys constitute the purification system for nucleic acid extraction.
The DNA glue reclaims experiment of Multi-example
Prepare：Sol solutionses：Guanidinium isothiocyanate 4M, potassium acetate 1M, glacial acetic acid adjust pH value to 5.0.
Rinsing liquid：10mM Tris-HCL, 80% alcoholic solution.
Eluent：10mM Tris-HCL, 1mM EDTA pH=8.0~8.5.
Experimental procedure：1st, single target DNA band is cut (cut off redundance as far as possible) from Ago-Gel and is put
Enter in clean centrifuge tube, weigh weight.
2nd, to 3 times of volume sol solutionses GSB are added in blob of viscose, (if gel weight is 0.1g, its volume can be considered 100 μ l, then
Add 300 μ lGSB solution), 65 DEG C of water-baths are placed, and constantly centrifuge tube are leniently spun upside down therebetween, to ensure that blob of viscose is fully molten
Solution.If there is not molten blob of viscose, can continue to place a few minutes or add some sol solutionses again, until blob of viscose be completely dissolved (if
The volume of blob of viscose is excessive, blob of viscose can be cut into fragment in advance)
3rd, centrifugal column is inserted into female Luer, opens extraction valve and simultaneously close off tapping valve, open vacuum filtration pump, will be solvent
Sol solution be all added in centrifugal column；
4th, until all of liquid is all by after the filter membrane of centrifugal column, adding 600 μ l rinsing liquids；
5th, until all of liquid is all by after the filter membrane of centrifugal column, adding 600 μ l rinsing liquids；
6th, until all of liquid is all by after the filter membrane of centrifugal column, evacuating 2~3min；By centrifugal column from female Luer
Remove, centrifugal column is placed in centrifuge tubes of the 1.5ml without RNase and DNase, to the wash-out that 35 μ l are added in the filter membrane of centrifugal column
Liquid, room temperature places 2min, 12000rpm centrifugation 1min, collects DNA, is stored in -20 DEG C or uses immediately.
The flux of negative pressure box of the present utility model is big in sum, conveniently topples over waste liquid, is placed on experimental bench or workbench
It is upper highly stable, it is simple to operate, it is not necessary to which that bottle,suction can reach and be directly connected to vacuum filtration pump, install and its simple, can be with
Received by vast scientific research clients；In addition, the apparatus cost is low, other negative pressure device prices than commercial type are low, have preferably
Market prospects.
Embodiment described above only expresses several embodiments of the present utility model, and its description is more specific and detailed,
But therefore can not be interpreted as the limitation to the utility model the scope of the claims.It should be pointed out that common for this area
For technical staff, without departing from the concept of the premise utility, various modifications and improvements can be made, these all belong to
In protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be determined by the appended claims.
Claims (8)
1. a kind of purification system for nucleic acid extraction, it is characterised in that：Including vacuum filtration pump and negative pressure device, the negative pressure
Device includes negative pressure box, tapping valve, extraction valve and female Luer, and the vacuum filtration pump connects extraction valve, the tapping valve, takes out
Air valve is arranged on negative pressure box both sides, and the female Luer is arranged on the upper surface of negative pressure box；The vacuum filtration pump is used to coordinate
Tapping valve and extraction valve extract negative pressure box internal gas.
2. a kind of purification system for nucleic acid extraction according to claim 1, it is characterised in that：The negative pressure box be by
The negative pressure box that one or more combination in high density polyethylene (HDPE) material, polypropylene, polystyrene is constituted.
3. a kind of purification system for nucleic acid extraction according to claim 2, it is characterised in that：The plate of the negative pressure box
Material thickness is 1.0cm~2.0cm.
4. a kind of purification system for nucleic acid extraction according to claim 2, it is characterised in that：The length of the negative pressure box
It is 25cm~35cm, a width of 12.5cm~14.5cm, a height of 9.5cm~10.5cm.
5. a kind of purification system for nucleic acid extraction according to claim 1, it is characterised in that：The female Luer exists
The spacing of negative pressure box upper surface is 1.5cm~3.0cm, and quantity is 12~48.
6. a kind of purification system for nucleic acid extraction according to claim 1, it is characterised in that：The position of the extraction valve
At the upper end 0.5cm~1cm of negative pressure box side, the position of the tapping valve is located at the lower end of extraction valve opposite side
At 0.5cm~1cm.
7. a kind of purification system for nucleic acid extraction according to claim 1, it is characterised in that：The purification system is also
Including centrifugal column, centrifugal column lower end can match female Luer, and volume is 1.5ml~50ml, and the thickness of Silicon moulds is 1mm.
8. a kind of purification system for nucleic acid extraction according to claim 1, it is characterised in that：The purification system bag
Three negative pressure devices are included, three negative pressure devices are connected with vacuum filtration pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621423079.XU CN206308344U (en) | 2016-12-22 | 2016-12-22 | A kind of purification system for nucleic acid extraction |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621423079.XU CN206308344U (en) | 2016-12-22 | 2016-12-22 | A kind of purification system for nucleic acid extraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206308344U true CN206308344U (en) | 2017-07-07 |
Family
ID=59245869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621423079.XU Active CN206308344U (en) | 2016-12-22 | 2016-12-22 | A kind of purification system for nucleic acid extraction |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206308344U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004233A (en) * | 2017-12-07 | 2018-05-08 | 上海睿玻生物科技有限公司 | A kind of negative pressure device available for automatic instrument for extracting nucleic acid |
| CN114854566A (en) * | 2022-06-01 | 2022-08-05 | 广东国盛医学科技有限公司 | Extraction and purification instrument and extraction and purification method |
-
2016
- 2016-12-22 CN CN201621423079.XU patent/CN206308344U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108004233A (en) * | 2017-12-07 | 2018-05-08 | 上海睿玻生物科技有限公司 | A kind of negative pressure device available for automatic instrument for extracting nucleic acid |
| CN114854566A (en) * | 2022-06-01 | 2022-08-05 | 广东国盛医学科技有限公司 | Extraction and purification instrument and extraction and purification method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A Purification System for Nucleic Acid Extraction Effective date of registration: 20230615 Granted publication date: 20170707 Pledgee: Industrial and Commercial Bank of China Limited Guangzhou Avenue sub branch Pledgor: GUANGZHOU IGE BIOTECHNOLOGY LTD. Registration number: Y2023980043872 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20170707 Pledgee: Industrial and Commercial Bank of China Limited Guangzhou Avenue sub branch Pledgor: GUANGZHOU IGE BIOTECHNOLOGY LTD. Registration number: Y2023980043872 |