CN107142202A - A kind of multifunction automatic biological cell extracts separation control system and the cell extraction separation method realized using the system - Google Patents
A kind of multifunction automatic biological cell extracts separation control system and the cell extraction separation method realized using the system Download PDFInfo
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Abstract
A kind of multifunction automatic biological cell extracts separation control system and the cell extraction separation method realized using the system, is related to blood product technical field.The present invention be in order to solve easily to be contaminated when extracting sample at present, the cell such as blood platelet be easily activated on unsuitable opportunity and influence therapeutic effect, and the existing mode efficiency for extracting sample it is low the problem of.Control signal output connects micro machine control signal input to double ECU chips all the way, the driving signal input of micro machine drive signal output end connecting eccentric wheel, eccentric wheel, which is rotated clockwise, is clipped in flow-stopping clip on woven hose, eccentric wheel, which is rotated counterclockwise, opens flow-stopping clip, normal saline bag respectively imports drop kettle one end with biological cell storage bag by 1 woven hose, drip the kettle other end and cell collecting bag and cell filter one end are connected by woven hose simultaneously, the cell filter other end connects syringe injection end and devil liquor recovery bag by woven hose simultaneously.It is separated for biological cells.
Description
Technical field
Separation control system and control method are extracted the present invention relates to a kind of biological cell, belongs to blood product technology neck
Domain.
Background technology
With the development of laboratory medicine and biotechnology, lymphocyte and platelet rich plasma in monocyte (MNC)
(PRP), the various factors in platelet lysates liquid (PL), are widely used for the multiple fields of scientific research, regenerative therapy.
Mononuclearcell can be with by induction, activation, quantity amplification or the step such as genetic modification under ultra clean environment
Particular kind of immunocyte is obtained, available for the adoptive treatment of tumour and targeted therapy.General method is by lymphocyte
Or antigen presenting cell is extracted, by processes such as in vitro culture, modification, activation, differentiation, its quantity and activity is set to obtain greatly
Width is improved, and is then fed back to again in patient's body, plays antiviral and antitumor action.
Rich in blood platelet technology for treatment of being festered caused by bone tissue reparation, burn treating, chronic ulcer and diabetes
Deng playing highly effective facilitation；In terms of beauty, PRP and PL carry color, removal pouch for wrinkle reduction, Firm, subtracted
Light scar, change pigmentation etc., also play an important role.
So mononuclearcell and the extraction quality rich in platelet products, be influence its function and effect it is crucial because
Element.
At present, filtration method also has been used to remove cell from blood, while retaining other blood constituents is used to then make
With.This method generally with expendable form by cell retention in filter, while allowing other blood constituents to pass through
Filter simultaneously enters collection vessel.For example, filter can be for removing leucocyte-removing from blood, therefore make of the same race after blood transfusion exempt from
The incidence of epidemic disease reaction is minimized.The typical way that leucocyte is removed is to use the filter being made up of plastic fiber net to carry out
's.Generally arrange the net that leucocyte is retained in the pseudostructure with adequate thickness so that cell retention is throughout filter
Thickness, therefore keep filter do not block, because if leucocyte is retained in plane surface and will blocked.
In addition to the Physical entrapment of cell, the material and high surface area of filter allow leucocyte irreversibly to adhere to
In surface.The cell thirsted in many exactly some medical care precesses in these adherent cells.Produced retention and adhere to
The combination of filter establishes the highly efficient process for removing leucocyte-removing before treatment of blood transfusion.However, when leucocyte is the phase
During the cell of prestige, this filter method is no advantage.
The content of the invention
The present invention is that when extracting sample, easily the cell such as contaminated, blood platelet is easy unsuitable in order to solve at present
Opportunity is activated and influences therapeutic effect, and it is existing extract sample mode efficiency it is low the problem of.A kind of many work(are now provided
Can automatic biological cell extraction separation control system and the cell extraction separation method using system realization.
A kind of multifunction automatic biological cell extracts separation control system, and it includes double ECU chips, micro machine, negative pressure
Isolating device 1, normal saline bag 2, biological cell storage bag 3 and disposable biological cell separation apparatus,
Disposable biological cell separation apparatus, which includes 6 current limliting folders, drop kettle 5, cell filter 6, cell filter, to be fixed
Folder 7, devil liquor recovery bag 8,10,6 current limliting folders of cell collecting bag 9 and syringe are respectively defined as current limliting folder 4-1, No. two limits
Stream folder 4-2, No. three current limliting folder 4-3, No. four current limliting folder 4-4, No. five current limlitings press from both sides 4-5 and No. six current limliting folder 4-6,
Negative-pressure isolation device 1 is the negative-pressure cofferdam 1-1 that rectangle is provided with body structure, casing,
Disposable biological cell separation apparatus is arranged in negative-pressure cofferdam 1-1 and on postnotum,
Each current limliting folder includes eccentric wheel 4-1-1, flow-stopping clip 4-1-2 and current limliting folder switch gate 4-1-3,
Current limliting folder switch gate 4-1-3 is rectangle cover structure, and micro machine is fixed on the postnotum inside negative-pressure cofferdam 1-1
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel 4-1-1 and flow-stopping clip 4-1-2 are arranged at square
In shape cover,
Double ECU chips are interconnected, the control signal input of double ECU chips control signal output connection micro machine all the way
End, the drive signal output end connecting eccentric wheel 4-1-1 of micro machine driving signal input, eccentric wheel 4-1-1's is clockwise
Flow-stopping clip 4-1-2 is clipped on woven hose by rotation, and flow-stopping clip 4-1-2 is opened in rotating counterclockwise for eccentric wheel 4-1-1,
Normal saline bag 2 respectively imports the one end for dripping kettle 5 with biological cell storage bag 3 by 1 woven hose, drips the another of kettle 5
One end connects one end of cell collecting bag 9 and cell filter 6 by woven hose simultaneously, and the other end of cell filter 6 passes through
Woven hose connects the injection end and devil liquor recovery bag 8 of syringe 10 simultaneously, and another road control signal output of double ECU chips connects
The push-in end of syringe 10 is connect,
Drop kettle 5 is used to observe decoction flow velocity,
A number current limliting folder 4-1 is arranged on the woven hose between normal saline bag 2 and drop kettle 5, and No. two current limliting folder 4-2 are set
On woven hose between biological cell storage bag 3 and drop kettle 5, No. three current limliting folder 4-3 are arranged on drop kettle 5 and cell filter 6
Between woven hose on, No. four current limlitings folder 4-4 are arranged on the woven hose between cell filter 6 and devil liquor recovery bag 8, No. five
Current limliting folder 4-5 is arranged on the woven hose between cell filter 6 and cell collecting bag 9, and No. six current limliting folder 4-6 are arranged on cell
On woven hose between filter 6 and syringe 10,
Cell filter 6 is fixed on by cell filter geometrical clamp 7 on negative-pressure cofferdam 1-1 backboard.
Beneficial effects of the present invention are：
The switch control pressed from both sides using disposable biological cell separation apparatus and the double ECU chips of combination to current limliting, in time, is had
Effect, neatly change filtration mode and filtration flow direction, are truly realized multifunction automatic, and have been greatly saved physical material resources, from
And add cell filtration efficiency, improve operation accuracy and reduce medical biologic contamination risk, different biologies can be directed to
The advantage that cell characteristics are efficiently filtered.Cell filtration efficiency improves more than 6 times compared with prior art.
The application can be with different into the presence of different cell filtering devices, realizing blood together with sleeving plastic multiple-bag
Composition separation, cell filtration, blood constituent preserve integration, prevent blood because opening causes germ contamination risk, it is to avoid because
The loss of biological cell caused by maloperation.
The application is provided with negative-pressure isolation device, in closed environment, is realized by air intake interface and air draft interface empty
Gas secure exchange, reaches the lasting controllable of particulate and microorganism in internal environment, and using express passway carry out biological cell and
The sterile transfer of medical disposable material, transfer, it is ensured that the protection of operator and biological cell.
Brief description of the drawings
Fig. 1 is the principle schematic of disposable biological cell separation apparatus；
Fig. 2 is the structural representation that current limliting is pressed from both sides；
Fig. 3 is the structural representation that drop kettle, infrared drop kettle folder and infrared drop kettle press from both sides sensor；
Fig. 4 is the structural representation of negative-pressure isolation device.
Embodiment
Embodiment one：Reference picture 1, Fig. 2 and Fig. 4 illustrate present embodiment, one described in present embodiment
Kind of multifunction automatic biological cell extracts separation control system, it include double ECU chips, micro machine, negative-pressure isolation device 1,
Normal saline bag 2, biological cell storage bag 3 and disposable biological cell separation apparatus,
Disposable biological cell separation apparatus, which includes 6 current limliting folders, drop kettle 5, cell filter 6, cell filter, to be fixed
Folder 7, devil liquor recovery bag 8,10,6 current limliting folders of cell collecting bag 9 and syringe are respectively defined as current limliting folder 4-1, No. two limits
Stream folder 4-2, No. three current limliting folder 4-3, No. four current limliting folder 4-4, No. five current limlitings press from both sides 4-5 and No. six current limliting folder 4-6,
Negative-pressure isolation device 1 is the negative-pressure cofferdam 1-1 that rectangle is provided with body structure, casing,
Disposable biological cell separation apparatus is arranged in negative-pressure cofferdam 1-1 and on postnotum,
Each current limliting folder includes eccentric wheel 4-1-1, flow-stopping clip 4-1-2 and current limliting folder switch gate 4-1-3,
Current limliting folder switch gate 4-1-3 is rectangle cover structure, and micro machine is fixed on the postnotum inside negative-pressure cofferdam 1-1
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel 4-1-1 and flow-stopping clip 4-1-2 are arranged at square
In shape cover,
Double ECU chips are interconnected, the control signal input of double ECU chips control signal output connection micro machine all the way
End, the drive signal output end connecting eccentric wheel 4-1-1 of micro machine driving signal input, eccentric wheel 4-1-1's is clockwise
Flow-stopping clip 4-1-2 is clipped on woven hose by rotation, and flow-stopping clip 4-1-2 is opened in rotating counterclockwise for eccentric wheel 4-1-1,
Normal saline bag 2 respectively imports the one end for dripping kettle 5 with biological cell storage bag 3 by 1 woven hose, drips the another of kettle 5
One end connects one end of cell collecting bag 9 and cell filter 6 by woven hose simultaneously, and the other end of cell filter 6 passes through
Woven hose connects the injection end and devil liquor recovery bag 8 of syringe 10 simultaneously, and another road control signal output of double ECU chips connects
The push-in end of syringe 10 is connect,
Drop kettle 5 is used to observe decoction flow velocity,
A number current limliting folder 4-1 is arranged on the woven hose between normal saline bag 2 and drop kettle 5, and No. two current limliting folder 4-2 are set
On woven hose between biological cell storage bag 3 and drop kettle 5, No. three current limliting folder 4-3 are arranged on drop kettle 5 and cell filter 6
Between woven hose on, No. four current limlitings folder 4-4 are arranged on the woven hose between cell filter 6 and devil liquor recovery bag 8, No. five
Current limliting folder 4-5 is arranged on the woven hose between cell filter 6 and cell collecting bag 9, and No. six current limliting folder 4-6 are arranged on cell
On woven hose between filter 6 and syringe 10,
Cell filter 6 is fixed on by cell filter geometrical clamp 7 on negative-pressure cofferdam 1-1 backboard.
In present embodiment, disposable biological cell separation apparatus is placed in inside negative-pressure cofferdam 1-1, negative-pressure cofferdam 1-
1 is completely enclosed aseptic processing environment.
Embodiment two：Present embodiment is biological to a kind of multifunction automatic described in embodiment one
Cell extraction separation control system is described further, in present embodiment, and each current limliting folder also includes detection sensor 4-1-
4,
Detection sensor 4-1-4 includes pressure sensor or ultrasonic bubble sensor,
Pressure sensor is used for the pressure for detecting transfusion liquid in pipe；
Ultrasonic bubble sensor is used for the bubbles volume and Air Bubble Size for detecting transfusion liquid in pipe.
Embodiment three：Reference picture 3 illustrates present embodiment, and present embodiment is to embodiment one
A kind of described multifunction automatic biological cell extracts separation control system and is described further, in present embodiment, and it is also
Sensor 11 and infrared drop kettle folder 12 are pressed from both sides including infrared drop kettle,
Infrared drop kettle folder 12 is used to be clipped in drop kettle 5, and infrared drop kettle presss from both sides 12 tops and is provided with infrared drop kettle folder sensor 11,
Infrared drop kettle folder sensor 11 is used to detect in drop kettle 5 whether have decoction instillation.
In this real-time mode, whether infrared drop kettle folder sensor 11 is used to detect has in drop kettle 5 in decoction instillation, drop kettle 5
Often drip down a drop of liquid, and infrared drop kettle folder sensor 11 will detect a signal, and every 20 drop is exactly that 20 signals are
1ml, is set according to inner parameter and does not detect signal when continuous 2-3 times, can alarm, and liquid may not be had in woven hose by informing
.
Infrared drop kettle folder sensor 11 includes infrared drop kettle and presss from both sides transmitting terminal and infrared drop kettle clamping connection receiving end, passes through infrared drop kettle
Transmitting terminal and infrared drop kettle clamping connection receiving end is pressed from both sides to detect the instillation of the drop inner liquid medicine of kettle 5.
Embodiment four：Present embodiment is biological to a kind of multifunction automatic described in embodiment one
Cell extraction separation control system is described further, in present embodiment, and the top of negative-pressure isolation device 1 is provided with air intake interface
13 and air draft interface 14, LED illumination lamp and ultraviolet sterilizing lamp built in negative-pressure isolation device 1,
Touching display screen 15 is installed on the front panel of negative-pressure isolation device 1, the push button signalling input of touching display screen 15/
The push button signalling input/output terminal of the double ECU chips of output end connection, negative-pressure cofferdam 1-1 front panel is clear glass, transparent
Two holes are provided with glass, two holes are tightly connected with a protection oversleeve gloves 16 respectively, protection oversleeve gloves 16
For being operated to the inside of negative-pressure isolation device 1.
In present embodiment, manhole is provided with the postnotum outside negative-pressure isolation device 1, for overhauling negative pressure isolation
Device 1.
Embodiment five：Present embodiment is biological to a kind of multifunction automatic described in embodiment four
Cell extraction separation control system is described further, in present embodiment, and it is sterile to be additionally provided with negative pressure in negative-pressure isolation device 1
Transferring cabin 1-2, is provided with a transmission channels door 17 between negative pressure sterile transfer cabin 1-2 and negative-pressure cofferdam 1-1.
In present embodiment, protection oversleeve gloves 16 are taken, in an aseptic environment, by normal saline bag 2, biological cell
Storage bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2.
Embodiment six：Present embodiment is biological to a kind of multifunction automatic described in embodiment five
Cell extraction separation control system is described further, in present embodiment, and it also includes warning device,
The induced signal output end of infrared drop kettle folder sensor 11, detection sensor 4-1-4 induced signal output end point
Not Lian Jie double ECU chips control signal input, the control signal output of double ECU chips connects the report of warning device respectively
Alert signal input part.
In present embodiment, it is to prevent an ECU wafer damage using the purpose of double ECU chips, can be entered with another
Row operation, normal operation is not influenceed.The value that sensor is collected is exported to double ECU chips, the setting with double ECU chip internals
Value is compared, if it is not the same, then being alarmed, and alarm signal is included in touch display screen.
Embodiment seven：Present embodiment is a kind of multifunction automatic life according to embodiment five
The biological cell that thing cell extraction separation control system is realized is extracted in separation control method, present embodiment, methods described bag
Include following steps：
Step 1: opening biological cell extracts piece-rate system, open ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed in from device after surface sterilizing inside negative pressure sterile transfer cabin 1-2；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be raw
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the negative-pressure cofferdam 1-1 inside back of the body
On plate, and fully exclude the liquid level in drop kettle 5；
Step 4: carrying out flushing of pipeline：Number current limliting folder 4-1 of control, 4-3 and No. four current limliting folder 4-4 of No. three current limlitings folders are opened
Open, other current limliting clip closures, the salt solution in normal saline bag 2 flows through drop kettle 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter：No. two current limliting folder 4-2 of control, No. three current limlitings press from both sides 4-3 and No. four current limliting folder 4-
4 open, other current limliting clip closures, and the blood in biological cell storage bag 3 flows through drop kettle 5 and cell filter 6, is returned into waste liquid
Closing bag 8；
Reversely collected Step 6: carrying out cell：No. five current limlitings of control press from both sides 4-5 and No. six current limliting folder 4-6 unlatching, other current limlitings
Clip closure, the control inverse injection reagent of syringe 10 flows through cell filter 6, into cell collecting bag 8, realizes cell extraction point
From.
Embodiment 1：
The biological cell extraction separation control that separation control system is realized is extracted according to a kind of multifunction automatic biological cell
Method processed, changes syringe 10 into cell collecting bag, the described method comprises the following steps：
Step 1: opening biological cell extracts piece-rate system, open ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed in from device after surface sterilizing inside negative pressure sterile transfer cabin 1-2；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be raw
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the negative-pressure cofferdam 1-1 inside back of the body
On plate, and fully exclude the liquid level in drop kettle 5；
Step 4: carrying out flushing of pipeline：Number current limliting folder 4-1 of control, 4-3 and No. four current limliting folder 4-4 of No. three current limlitings folders are opened
Open, other current limliting clip closures, the salt solution in normal saline bag 2 flows through drop kettle 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter：No. two current limliting folder 4-2 of control, No. three current limlitings press from both sides 4-3 and No. six current limliting folder 4-
6 open, other current limliting clip closures, and the blood in biological cell storage bag 3 flows through drop kettle 5 and cell filter 6, is received into cell
Collect bag, realize that cell is collected.
Embodiment 2：
The biological cell extraction separation control that separation control system is realized is extracted according to a kind of multifunction automatic biological cell
Method processed, the described method comprises the following steps：
Step 1: opening biological cell extracts piece-rate system, open ultraviolet sterilizing lamp and carry out ultraviolet sterilization function
30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag 2, biological cell storage bag 3 and disposable biological cell point
It is placed in from device after surface sterilizing inside negative pressure sterile transfer cabin 1-2；
Step 3: normal saline bag 2, biological cell are stored by transmission channels door 17 with oversleeve gloves 16 using protection
Bag 3 and disposable biological cell separation apparatus are moved in negative-pressure cofferdam 1-1 from negative pressure sterile transfer cabin 1-2, then will be raw
Reason bag of saline 2, biological cell storage bag 3 and disposable biological cell separation apparatus are installed to the negative-pressure cofferdam 1-1 inside back of the body
On plate, and fully exclude the liquid level in drop kettle 5；
Step 4: carrying out flushing of pipeline：Number current limliting folder 4-1 of control, 4-3 and No. four current limliting folder 4-4 of No. three current limlitings folders are opened
Open, other current limliting clip closures, the salt solution in normal saline bag 2 flows through drop kettle 5 and cell filter 6, into devil liquor recovery bag 8；
Step 5: carrying out cell forward filter：No. five current limlitings of control press from both sides 4-5 and No. six current limliting folder 4-6 unlatching, other current limlitings
Folder 4 is closed, and the control injection reagent of syringe 10 flows through cell filter 6, into cell collecting bag 9, then extracts as needed
Sample is detected in cell collecting bag 9.
Claims (7)
1. a kind of multifunction automatic biological cell extracts separation control system, it is characterised in that it includes double ECU chips, micro-
Motor, negative-pressure isolation device (1), normal saline bag (2), biological cell storage bag (3) and disposable biological cell separation apparatus,
Disposable biological cell separation apparatus, which includes 6 current limliting folders, drop kettle (5), cell filter (6), cell filters, to be fixed
(7), devil liquor recovery bag (8), cell collecting bag (9) and syringe (10) are pressed from both sides, 6 current limliting folders are respectively defined as a current limliting folder
(4-1), No. two current limliting folders (4-2), No. three current limliting folders (4-3), No. four current limliting folders (4-4), No. five current limliting folders (4-5) and No. six limits
Stream folder (4-6),
Negative-pressure isolation device (1) is the negative-pressure cofferdam (1-1) that rectangle is provided with body structure, casing,
Disposable biological cell separation apparatus is arranged in negative-pressure cofferdam (1-1) and on postnotum,
Each current limliting folder includes eccentric wheel (4-1-1), flow-stopping clip (4-1-2) and current limliting folder switch gate (4-1-3),
Current limliting folder switch gate (4-1-3) is rectangle cover structure, and micro machine is fixed on the internal postnotum of negative-pressure cofferdam (1-1)
On, rectangular cover is fixed on the postnotum of box house, and micro machine, eccentric wheel (4-1-1) and flow-stopping clip (4-1-2) are respectively provided with
In rectangular cover,
Double ECU chips are interconnected, and control signal output connects the control signal input of micro machine to double ECU chips all the way,
The driving signal input of the drive signal output end connecting eccentric wheel (4-1-1) of micro machine, eccentric wheel (4-1-1) it is clockwise
Flow-stopping clip (4-1-2) is clipped on woven hose by rotation, and flow-stopping clip (4-1-2) is opened in rotating counterclockwise for eccentric wheel (4-1-1),
Normal saline bag (2) respectively imports one end of drop kettle (5), drop kettle (5) with biological cell storage bag (3) by 1 woven hose
The other end by woven hose simultaneously connect cell collecting bag (9) and cell filter (6) one end, cell filter (6)
The other end connects the injection end and devil liquor recovery bag (8) of syringe (10), another road control of double ECU chips by woven hose simultaneously
The push-in end of signal output part connection syringe (10) processed,
Drop kettle (5) is used to observe decoction flow velocity,
A number current limliting folder (4-1) is arranged on the woven hose between normal saline bag (2) and drop kettle (5), and No. two current limlitings press from both sides (4-2)
Be arranged on the woven hose between biological cell storage bag (3) and drop kettle (5), No. three current limlitings folders (4-3) be arranged on drop kettle (5) with
On woven hose between cell filter (6), No. four current limliting folders (4-4) are arranged on cell filter (6) and devil liquor recovery bag (8)
Between woven hose on, No. five current limlitings folder (4-5) is arranged on the woven hose between cell filter (6) and cell collecting bag (9)
On, No. six current limliting folders (4-6) are arranged on the woven hose between cell filter (6) and syringe (10),
Cell filter (6) is fixed on negative-pressure cofferdam (1-1) backboard by cell filter geometrical clamp (7).
2. a kind of multifunction automatic biological cell according to claim 1 extracts separation control system, it is characterised in that
Each current limliting folder also includes detection sensor (4-1-4),
Detection sensor (4-1-4) includes pressure sensor or ultrasonic bubble sensor,
Pressure sensor is used for the pressure for detecting transfusion liquid in pipe；
Ultrasonic bubble sensor is used for the bubbles volume and Air Bubble Size for detecting transfusion liquid in pipe.
3. a kind of multifunction automatic biological cell according to claim 1 extracts separation control system, it is characterised in that
It also includes infrared drop kettle folder sensor (11) and infrared drop kettle folder (12),
Infrared drop kettle folder (12) is used to be clipped in drop kettle (5), and infrared drop kettle folder (12) top is provided with infrared drop kettle folder sensor
(11), infrared drop kettle folder sensor (11) is used to detect in drop kettle (5) whether have decoction instillation.
4. a kind of multifunction automatic biological cell according to claim 1 extracts separation control system, it is characterised in that
The top of negative-pressure isolation device (1) is provided with air intake interface (13) and air draft interface (14), and LED built in negative-pressure isolation device (1) shines
Bright lamp and ultraviolet sterilizing lamp,
Touching display screen (15) is installed, the push button signalling of touching display screen (15) is defeated on the front panel of negative-pressure isolation device (1)
Enter/push button signalling the input/output terminal of the double ECU chips of output end connection, the front panel of negative-pressure cofferdam (1-1) is transparent glass
Two holes are provided with glass, clear glass, two holes are tightly connected with a protection with oversleeve gloves (16) respectively, protection sleeve
Set gloves (16) are used for being operated inside negative-pressure isolation device (1).
5. a kind of multifunction automatic biological cell according to claim 4 extracts separation control system, it is characterised in that
Negative pressure sterile transfer cabin (1-2), negative pressure sterile transfer cabin (1-2) and negative-pressure cofferdam are additionally provided with negative-pressure isolation device (1)
A transmission channels door (17) is provided between (1-1).
6. a kind of multifunction automatic biological cell according to claim 5 extracts separation control system, it is characterised in that
It also includes warning device,
The induced signal output end of infrared drop kettle folder sensor (11), the induced signal output end point of detection sensor (4-1-4)
Not Lian Jie double ECU chips control signal input, the control signal output of double ECU chips connects the report of warning device respectively
Alert signal input part.
7. the biology that a kind of multifunction automatic biological cell according to claim 5 extracts separation control system realization is thin
Born of the same parents extract separation control method, it is characterised in that the described method comprises the following steps：
Step 1: opening biological cell extracts piece-rate system, open ultraviolet sterilizing lamp and carry out ultraviolet sterilization function 30min；
Step 2: ultraviolet sterilization lamp is closed, by normal saline bag (2), biological cell storage bag (3) and disposable biological cell point
Negative pressure sterile transfer cabin (1-2) inside is placed in after surface sterilizing from device；
Step 3: normal saline bag (2), biological cell are deposited by transmission channels door (17) with oversleeve gloves (16) using protection
Bag (3) and disposable biological cell separation apparatus are moved in negative-pressure cofferdam (1-1) from negative pressure sterile transfer cabin (1-2),
Then normal saline bag (2), biological cell storage bag (3) and disposable biological cell separation apparatus are installed to negative-pressure cofferdam
On the inside backboard of (1-1)；
Step 4: carrying out flushing of pipeline：Number current limliting folder (4-1) of control, No. three current limliting folders (4-3) and No. four current limliting folders (4-4)
Open, other current limliting clip closures, the salt solution in normal saline bag (2) flows through drop kettle (5) and cell filter (6), into waste liquid
Collection bag (8)；
Step 5: carrying out cell forward filter：No. two current limliting folders (4-2) of control, No. three current limliting folders (4-3) and No. four current limliting folders
(4-4) is opened, other current limliting clip closures, and the blood in biological cell storage bag (3) flows through drop kettle (5) and cell filter (6),
Into devil liquor recovery bag (8)；
Reversely collected Step 6: carrying out cell：No. five current limliting folders (4-5) of control and No. six current limliting folders (4-6) are opened, other current limlitings
Clip closure, control syringe (10) inverse injection reagent flows through cell filter (6), into cell collecting bag (8), realizes cell
Extract separation.
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| CN113509607A (en) * | 2021-04-23 | 2021-10-19 | 温州医科大学附属第一医院 | Pulmonary artery high-pressure cardiac hybrid radiography auxiliary device |
| WO2023241597A1 (en) * | 2022-06-14 | 2023-12-21 | 南京金斯瑞生物科技有限公司 | Pipeline system for cell sorting, cell sorting device, and control method |
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| CN107142202B (en) | 2019-10-08 |
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Address after: 150001 No. 199, Jubao No. 2 Road, Science and Technology Innovation City, Harbin Hi-tech Industrial Development Zone, Heilongjiang Province Applicant after: Sunny stem cell Limited by Share Ltd Address before: The innovation of science and Technology City giant - Harbin high tech Industrial Development Zone, Heilongjiang province Harbin city two road 150028 No. 199 Applicant before: HEILONGJIANG TIANQING STEM CELL CO., LTD. |
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