CN207877718U - The micro-fluidic sampling device of bubble-free of cell solution - Google Patents
The micro-fluidic sampling device of bubble-free of cell solution Download PDFInfo
- Publication number
- CN207877718U CN207877718U CN201820084593.8U CN201820084593U CN207877718U CN 207877718 U CN207877718 U CN 207877718U CN 201820084593 U CN201820084593 U CN 201820084593U CN 207877718 U CN207877718 U CN 207877718U
- Authority
- CN
- China
- Prior art keywords
- bubble
- solution
- cell
- micro
- buffer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The utility model discloses a kind of micro-fluidic sampling devices of the bubble-free of cell solution, including cell sample injection unit, bubble reflux unit and buffer solution auxiliary unit；The cell sample injection unit includes cell solution pipe, pushes the first force (forcing) pump for being flowed to bubble reflux unit of cell solution and the sterile air filter in pipe；Buffer solution auxiliary unit includes buffer solution bottle, the second force (forcing) pump and solution sterilizing filter for pushing the buffer solution in bottle to be flowed to bubble reflux unit；Bubble reflux unit includes bubble reflux column, and by being connected to cell solution pipe and micro-fluidic chip by the first valve, cylinder top connects buffer solution bottle and pressure relief valve by the second valve for cylinder lower part.The device can easily remove the bubble in cell solution, it avoids micro-fluidic chip from introducing bubble when separation is with capture cell and influences separative efficiency, and automation control can be realized, to provide facility for biology and medical research, biochemical instrument manufacture and clinical application.
Description
Technical field
The utility model is related to a kind of micro-fluidic sampling device more particularly to a kind of bubble-free of cell solution it is micro-fluidic into
Sampling device belongs to biotechnology engineering and medical domain.
Background technology
Cell separation, identification and its molecule diagnosis are biology and one of the substance of medical research and its clinical application.
With the development of modern age micro Process and material technology, microfluidic chip technology has become biological study and medical research and faces
The lossless separation of the most potential cell of bed application and capture technique.Currently, cell is detached and captured using micro-fluidic chip
Representative art principle is that target cell antibody is fixed in micro-fluidic chip, when cell solution flows through micro-fluidic chip, core
The fixed antibody of piece is combined with target cell surface antigentic specificity, to realize separation and capture to target cell.It is clear that
Realize that contact of the target cell with antibody is the key point for influencing micro-fluidic chip and detaching and capturing cell efficiency to the maximum extent,
In this respect, the bubble in cell solution is the biggest impact factor for influencing separation and capture rate, and Air Bubble Size is far longer than
Cell size, if there is bubble in cell solution, when flowing through micro-fluidic chip, then bubble will shield contact of the cell with antibody,
To which separation and the capture rate of target cell be greatly lowered.Therefore, after cell solution needs to exclude bubble therein first
Micro-fluidic chip could be entered.
Since cell is different from compound, cannot micro/nano level duct be directly set in the duct and leads to the bubble in solution
Filter type exclusion is crossed, because this will make cytoclasis simultaneously.In addition, cell is active, bubble in cell solution is excluded
While cell cannot be caused it is any physically or chemically aspect damage.Therefore, it is detached and is captured carefully using micro-fluidic chip
Before born of the same parents, such as how undamaged mode removes bubble therein, that is, bubble-free sample introduction is micro-fluidic chip using urgently to be resolved hurrily
One of which technical problem.
Utility model content
Purpose of utility model：The utility model be intended to provide it is a kind of can realize the cell solution of bubble-free sample introduction it is micro-fluidic into
Sampling device.
Technical solution：The micro-fluidic sampling device of bubble-free of cell solution described in the utility model, including cell sample introduction
Unit, bubble reflux unit and buffer solution auxiliary unit；Wherein, the cell sample injection unit includes cell solution pipe, pushes pipe
The first force (forcing) pump and sterile air filter that interior cell solution is flowed to bubble reflux unit；The buffer solution auxiliary unit
Including buffer solution bottle, the second force (forcing) pump and solution sterilizing filter that push the buffer solution in bottle to be flowed to bubble reflux unit；
The bubble reflux unit includes bubble reflux column, and cylinder lower part is connected by the first valve and cell solution pipe and micro-fluidic chip
Logical, cylinder top connects buffer solution bottle and pressure relief valve by the second valve.
Preferably, the cell solution pipe is equipped with sealing cover, and the first force (forcing) pump and sterile air filter pass through first
Branch pipe is connected to and stretches into the branch pipe in sealing cover；Bubble reflux column lower part is accessed in second branch pipe one end by the first valve, separately
One end is stretched into across sealing cover in cell solution.
Preferably, buffer solution bottle sealing cover is set on the buffer solution bottle, it is close that the second force (forcing) pump by third branch pipe accesses this
In capping；One end of 4th branch pipe is stretched into across buffer solution bottle sealing cover in buffer solution, and the other end connects sterile air filter,
Sterile air filter accesses the second valve by the 5th branch pipe, to be connected to bubble reflux column top.
Further, the sealing cover is diplopore rubber packing lid.Branch pipe is PTFE tube.First valve and the second valve are
Three-way valve.Sterile air filter and solution sterilizing filter are membrane filter (filter sizes are 0.1~0.22 μm).
The cylinder length of bubble reflux column is 5~15cm.
Operation principle：The cell sample injection unit of the utility model, cell enter micro-fluidic chip by filtrated air pressurization,
It not only can guarantee that cell solution is not contaminated, but also air pressurized not exposing cell, thus be not damaged to the cell in solution
's；Buffer solution enters pipeline with pressuring method, identical as cell solution input mode, this is beneficial to Row control, and delays
Fliud flushing is filtered through nano level solution strainer, is avoided the pollution that buffer solution may bring cell, is protected cell sample；
The setting of buffer solution auxiliary unit can also in advance be excluded the bubble in pipeline, to avoid excluding the process of bubble
Middle loss cell sample；Cell solution is connected by bubble reflux column with buffer solution, and cell not only can be made to enter
Can be by the bubble in the advance purging line of buffer solution before micro-fluidic chip, and it can be to the remnants in cell solution
Bubble realizes secondary removal during sample introduction；In addition, pressure relief valve is arranged in bubble reflux column one end, can avoid pressurizeing into
Pressure may be excessive and make cell and equipment damage during sample.
Advantageous effect：Compared with prior art, it is the advantages of the utility model：The device be for micro-fluidic chip separation with
Capture cell design corollary apparatus, can easily remove the bubble in cell solution, avoid micro-fluidic chip separation with
Bubble is introduced when capturing cell and influences separative efficiency, and the device can realize automation control, to be biology and doctor
It learns research, biochemical instrument manufacture and clinical application and facility is provided.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model.
Specific implementation mode
The technical solution of the utility model is described further below in conjunction with the accompanying drawings.
As shown in Figure 1, the micro-fluidic sampling device of the bubble-free of the utility model, including cell sample injection unit 1, bubble reflux
Unit 2 and buffer solution auxiliary unit 3, three units shown in dotted line in figure.
Wherein, cell sample injection unit 1 includes cell solution pipe 101, the first force (forcing) pump 102 and sterile air filter 103,
First force (forcing) pump 102 can push the cell solution in pipe to be flowed to bubble reflux unit 2, and cell solution pipe 101 is equipped with first
Sealing cover 104, preferably sealing cover are diplopore rubber packing lid, and the first force (forcing) pump 102 and sterile air filter 103 pass through
First branch pipe 105 is connected to and stretches into first branch pipe 105 in first sealing cover 104；Second branch pipe, 106 one end passes through first
Valve 203 accesses 201 lower part of bubble reflux column, and the other end is stretched into across the first sealing cover 104 in cell solution, wherein first
Branch pipe, the second branch pipe are preferably PTFE tube, and it is 0.5mm that pipe diameter, which may be selected to be internal diameter, and length can be according to the big minor adjustment of device.
Buffer solution auxiliary unit 3 include buffer solution bottle 301, the second force (forcing) pump 302 and solution sterilizing filter 303, second
Force (forcing) pump 302 can push the buffer solution in bottle to be flowed to bubble reflux unit 2, wherein buffer solution is PBS (pH 7.4), is delayed
The second sealing cover 304 is set on fliud flushing bottle 301, preferably sealing cover is diplopore rubber packing lid, and the second force (forcing) pump 302 passes through third
Branch pipe 305 accesses in the second sealing cover 304；One end of 4th branch pipe 306 is stretched into across the second sealing cover 304 in buffer solution, separately
One end connects solution sterilizing filter 303, the solution sterilizing filter 303 by the 5th branch pipe 307 access the second valve 204 from
And it is connected to 201 top of bubble reflux column, wherein third branch pipe, the 4th branch pipe and the 5th branch pipe are preferably PTFE tube, pipe diameter
May be selected to be internal diameter is 0.5mm, and length can be according to the big minor adjustment of device.
Bubble reflux unit 2 includes a bubble reflux column 201 being vertically arranged, and cylinder lower part connects the first valve 203,
It is connected to cell solution pipe by the second branch pipe 106, while the 6th branch pipe 205 and micro-fluidic core is passed through by the first valve 203
Piece is connected to, and cylinder top connects buffer solution bottle 301 and pressure relief valve 202 by the second valve 204.Wherein, the first valve 203
It is preferably three-way valve with the second valve 204, which is 0.5mm, and has pole changer, can be connected arbitrary
Two channels；Pressure relief valve is uniaxial pressure regulating valve, can be discharged when manifold pressure is more than 1.5 atmospheric pressure more
Overbottom pressure power；The internal diameter of bubble reflux column be 5mm, character be it is cylindric, can be glass or plastic tube, cylinder length be 5~
15cm。
102 and second force (forcing) pump 302 of above-mentioned first force (forcing) pump can be micro force (forcing) pump, and can reach minimum 0.1 μ L/ seconds
Air sample size.Sterile air filter 103 and solution sterilizing filter 303 are membrane filter (filter sizes 0.1
~0.22 μm).
The course of work：
The first step：The advance purging line bubble of buffer solution：
1. controlling the steering of the second valve 204, the 5th branch pipe 307 and bubble reflux column 201 is made to communicate；
2. controlling the steering of the first valve 203, the second branch pipe 106 and bubble reflux column 201 is made to communicate；
3. starting the second force (forcing) pump 302, buffer solution pressurization is made quickly to be sterile filtered followed by the 4th branch pipe 306, solution
Device 303, the 5th branch pipe 307, the second valve 204, bubble reflux column 201, the first valve 203 and the 6th branch pipe 205, and from the 6th
It is flowed out in branch pipe 205, by increasing the bubble in flow velocity exclusion channel.
Second step：Cell solution quantitatively removes bubble removing into bubble reflux column：
1. controlling the steering of the first valve 203, the second branch pipe 106 and bubble reflux column 201 is made to communicate；
2. calculating pipeline cavity volume V according to the length of the second branch pipe 106 and inner diameter size；
3. starting the first force (forcing) pump 102, air is pressurized to after sterilizing filter 103 in cell solution pipe 101, makes cell
Solution rapidly enters the second branch pipe 106, the first valve 203, bubble reflux column 201, according to the flow velocity of setting, until cell solution
After reaching 2V volumes into total amount, the first force (forcing) pump 102 is closed；
4. starting the second force (forcing) pump 302, buffer solution is made to enter bubble reflux column 201, pressure relief valve 202 excludes bubble；
5. closing the second force (forcing) pump 302.
Third walks：The cell solution of bubble-free enters micro-fluidic chip：
1. the 6th branch pipe 205 connects micro-fluidic chip injection port；
2. starting the second force (forcing) pump 302, according to the flow velocity of setting, the cell solution of not bubbles is made to enter micro-fluidic core
Piece；
3. after the completion of cell solution sample introduction, closing the first force (forcing) pump 102；
4. starting the second force (forcing) pump 302, by the promotion of buffer solution, cell solution remaining in pipeline is made to be all introduced into
Micro-fluidic chip.
Embodiment 1：Sample introduction parameter
Inventor has detected lower cell solution different in flow rate from by taking the leucocyte solution of people as an example, according to same step
Bubble when six branch pipes 205 flow out there are situation, the specific steps are：
1, healthy person peripheral blood 1mL is collected using vacuum anti-freezing (EDTA) heparin tube；
2, red blood cell is removed：Using erythrocyte cracked liquid (NH containing 154mM4Cl、10mM KHCO3And 0.1mM EDTA
Ultra-pure water solution) removal red blood cell therein；
3, using PBS buffer solution (pH, 7.4), leucocyte is configured to 1x105The cell solution of/mL；
4,0.5mL leucocyte solution is taken to intervene cell sampling system respectively；
5, the first step of step as described above, passes through the advance purging line bubble of buffer solution first；
6, the second step of step, pressure cell solution 10sec as described above make about 20 μ L cell solutions enter the
Two branch pipes 106 and bubble reflux column 201；
7, the third step of step as described above, respectively with 5,6,7,8,9,10,20,30,40,50,100,200,
500 μ L/min flow velocitys, when test cell solution is flowed out from the 6th branch pipe 205 bubble there are situations, and count the damage of leucocyte
Lose situation.Wherein, method determines by visual observation for bubble presence or absence.
As a result (being shown in Table 1) is shown, when flow velocity is less than 5 μ L/min, with the presence of a small amount of remaining micro-bubble, but second
When the end of the step, 1min is stood by bubble reflux column by is easily excluded completely.When flow velocity is more than 5 μ L/min, the 6th
The outflow port of branch pipe 205 has no that bubble exists.Under all flow velocitys, cell solution can 100% realization bubble removal without
Loss.Since the flow rates tested cover micro-fluidic chip correlation test, thus the above results show that the device can
Quickly, bubble removal that is efficient, lossless and conveniently realizing cell solution, enters miniflow so as to avoid the bubble in solution
Control chip and caused by separative efficiency reduce.
Bubble result in the lower described device removal cell solution different in flow rate of table 1
。
Claims (8)
1. a kind of micro-fluidic sampling device of the bubble-free of cell solution, it is characterised in that：Including cell sample injection unit (1), bubble
Reflux unit (2) and buffer solution auxiliary unit (3)；Wherein, the cell sample injection unit (1) includes cell solution pipe (101), pushes away
The first force (forcing) pump (102) and sterile air filter (103) that cell solution in dynamic pipe is flowed to bubble reflux unit (2)；
The buffer solution auxiliary unit (3) includes buffer solution bottle (301), and the buffer solution in bottle is pushed to be flowed to bubble reflux unit (2)
The second force (forcing) pump (302) and solution sterilizing filter (303)；The bubble reflux unit (2) includes bubble reflux column
(201), cylinder lower part is connected to cell solution pipe and micro-fluidic chip respectively by the first valve (203), and cylinder top passes through
Second valve (204) is separately connected buffer solution bottle and pressure relief valve (202).
2. the micro-fluidic sampling device of bubble-free according to claim 1, it is characterised in that：The cell solution pipe (101)
The first sealing cover (104), the first force (forcing) pump (102) and sterile air filter (103) is equipped with by the first branch pipe (105) to connect
Lead to and stretches into the branch pipe in sealing cover；Bubble reflux column is accessed in second branch pipe (106) one end by the first valve (203)
(201) lower part, the other end are stretched into across sealing cover in cell solution.
3. the micro-fluidic sampling device of bubble-free according to claim 1, it is characterised in that：On the buffer solution bottle (301)
If the second sealing cover (304), the second force (forcing) pump (302) is accessed by third branch pipe (305) in the sealing cover；4th branch pipe
(306) one end is stretched into across buffer solution bottle sealing cover in buffer solution, and the other end connects solution sterilizing filter (303), solution
Sterilizing filter (303) accesses the second valve (204) by the 5th branch pipe (307), to be connected on bubble reflux column (201)
Portion.
4. the micro-fluidic sampling device of bubble-free according to claim 2 or 3, it is characterised in that：The sealing cover is diplopore
Rubber packing lid.
5. the micro-fluidic sampling device of bubble-free according to claim 2 or 3, it is characterised in that：The branch pipe is PTFE tube.
6. the micro-fluidic sampling device of bubble-free according to claim 1, it is characterised in that：First valve (203) and
Second valve (204) is three-way valve.
7. the micro-fluidic sampling device of bubble-free according to claim 1, it is characterised in that：The sterile air filter
(103) and solution sterilizing filter (303) is membrane filter, wherein the aperture of filter is 0.1~0.22 μm.
8. the micro-fluidic sampling device of bubble-free according to claim 1, it is characterised in that：The bubble reflux column (201)
Cylinder length be 5~15cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820084593.8U CN207877718U (en) | 2018-01-18 | 2018-01-18 | The micro-fluidic sampling device of bubble-free of cell solution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820084593.8U CN207877718U (en) | 2018-01-18 | 2018-01-18 | The micro-fluidic sampling device of bubble-free of cell solution |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207877718U true CN207877718U (en) | 2018-09-18 |
Family
ID=63507438
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820084593.8U Active CN207877718U (en) | 2018-01-18 | 2018-01-18 | The micro-fluidic sampling device of bubble-free of cell solution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207877718U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110095624A (en) * | 2019-04-25 | 2019-08-06 | 广州安方生物科技有限公司 | The device and method of bubble detection and removal when a kind of sample introduction |
| CN114441274A (en) * | 2022-01-27 | 2022-05-06 | 武汉生民医疗科技有限公司 | Cell staining system, staining control method and related equipment |
| CN114480072A (en) * | 2022-01-27 | 2022-05-13 | 武汉生民医疗科技有限公司 | Cell capture system, method of controlling capture, and related apparatus |
-
2018
- 2018-01-18 CN CN201820084593.8U patent/CN207877718U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110095624A (en) * | 2019-04-25 | 2019-08-06 | 广州安方生物科技有限公司 | The device and method of bubble detection and removal when a kind of sample introduction |
| CN110095624B (en) * | 2019-04-25 | 2023-03-21 | 广州安方生物科技有限公司 | Device and method for detecting and removing bubbles during sample injection |
| CN114441274A (en) * | 2022-01-27 | 2022-05-06 | 武汉生民医疗科技有限公司 | Cell staining system, staining control method and related equipment |
| CN114480072A (en) * | 2022-01-27 | 2022-05-13 | 武汉生民医疗科技有限公司 | Cell capture system, method of controlling capture, and related apparatus |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN207877718U (en) | The micro-fluidic sampling device of bubble-free of cell solution | |
| TW201329231A (en) | Method and device for isolation of non-fat cells from an adipose tissue | |
| JP2006329728A (en) | Specimen collection liquid container | |
| JP4492396B2 (en) | Sample collection liquid container | |
| JP2013514806A (en) | Apparatus and method for purification and concentration of biological samples | |
| CN107142202B (en) | A kind of cell extraction separation method that multifunction automatic biological cell is extracted separation control device and realized using the device | |
| CN109234152B (en) | Circulating tumor cell separator | |
| JPWO2017154349A1 (en) | Nucleic acid extraction apparatus, nucleic acid extraction unit and nucleic acid extraction method | |
| CN205460062U (en) | Thorax hydrops collection device | |
| TWM627564U (en) | Closed Cell Separation Operation Device | |
| CN109157228A (en) | A kind of heparin tube of instant serum separation | |
| CN113375983A (en) | Sterile sampling device and sampling method thereof | |
| CN212807652U (en) | Fecal sample collection device with one-way valve | |
| CN110411787B (en) | A collection processing apparatus for clinical laboratory's urine sample | |
| CN208350448U (en) | A kind of clinical laboratory quantifies fungi-proofing quick acquisition device | |
| JP2006280318A (en) | Separating and recovering method | |
| CN103452553A (en) | Multifunctional oil-gas well casing gas-liquid separating, filtering and drying device | |
| CN108744988B (en) | Air filter/membrane liquid bacteria interception capability test module for transfusion | |
| CN114276894A (en) | Air pressure driven type rapid nucleic acid separation, purification, concentration and detection device | |
| CN220339752U (en) | Dialysate sampling device | |
| CN215339083U (en) | Sterile sampling device | |
| CN205749024U (en) | Adsorption filtration formula random urine sample harvester | |
| CN212964299U (en) | Pretreatment device for solid tissue sample | |
| CN112774742B (en) | Blood cell filters washing unit | |
| CN218879904U (en) | Antigen biological sample sampling tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |