CN208121116U - A kind of device of paramagnetic particle method rapidly extracting nucleic acid - Google Patents
A kind of device of paramagnetic particle method rapidly extracting nucleic acid Download PDFInfo
- Publication number
- CN208121116U CN208121116U CN201721793201.7U CN201721793201U CN208121116U CN 208121116 U CN208121116 U CN 208121116U CN 201721793201 U CN201721793201 U CN 201721793201U CN 208121116 U CN208121116 U CN 208121116U
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- lid
- nucleic acid
- suction nozzle
- annular
- particle method
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of devices of paramagnetic particle method rapidly extracting nucleic acid, syringe-like main body includes tubular body, push rod, suction nozzle is sealed by a matched suction nozzle cap, suction nozzle is equipped with an annular slot, one end of annular slot is equipped with the scarf for hindering the piston of push rod to push away to the lower part, annular magnet includes half-ring-shaped magnets and semi-circular non-magnetic material, half-ring-shaped magnets are connected with semi-circular non-magnetic material one end by shaft, the other end can be connected or be split, closed sample filter cartridge includes cartridge body, lid, screw thread on lid can be screwed sealing with cartridge body inner wall, there is the sieve of an entire inner section of covering lid in the inside of lid, cap top has the liquid outlet of a protrusion, there are a frosted or label paper paste area in cartridge body and lid.The utility model has the beneficial effects that paramagnetic particle method rapidly extracting nucleic acid equipment is simple, price is low.
Description
Technical field
The utility model belongs to experiment equipment and manufacture technology field of medical appliance, is related to a kind of paramagnetic particle method rapidly extracting core
The device of acid.
Background technique
Scientific research personnel or medical personnel usually need to extract the nucleic acid of various samples, then carry out nucleic acid amplification and identify it
In microorganism, and then detect microorganism distribution or diagnosis is made to disease.It is various due to containing in various samples
Organic matter and inorganic matter, these substances can inhibit the enzyme in nucleic acid amplification reaction, carry out its reaction can not, therefore in nucleic acid amplification
It is preceding to need to purify the nucleic acid in sample.Common sample type has body fluid, cotton swab, tissue, excrement, soil etc..?
It before carrying out nucleic acid extraction, needs to pre-process sample, different sample processing methods is different.Scientific research personnel and doctor at present
The author that works carries out the nucleic acid extraction of sample, relies primarily on complicated kit and instrument and equipment expensive in laboratory,
Basic medical unit is often difficult to bear these costs, and cannot achieve the quick diagnosis at scene.One is still needed to currently on the market
Kind structure is simple and convenient to operate and is suitable for the high solubility of various samples, purity nucleic acid-extracting apparatus.
Utility model content
The purpose of this utility model is to provide a kind of device of paramagnetic particle method rapidly extracting nucleic acid, solves and carry out core at present
Structure is complicated for the laboratory equipment that acid extraction uses, expensive problem.
Technical solution used by the utility model be include syringe-like main body, annular magnet, sample filtering box；Injection
Device sample main body includes tubular body, push rod, and suction nozzle is sealed by a matched suction nozzle cap, and suction nozzle is equipped with an annular slot, annular snap
One end of slot is equipped with the scarf for hindering the piston of push rod to push away to the lower part, and annular magnet includes that half-ring-shaped magnets and semi-circular are non-magnetic
Property material, half-ring-shaped magnets are connected with semi-circular non-magnetic material one end by shaft, the other end can be connected or be split, connection
When by snapping connection, annular diameters are identical as annular slot outer diameter upon connection for annular magnet, closed sample filter cartridge
Including cartridge body, lid, the screw thread on lid can be with the sealing that is screwed of cartridge body inner wall, and the inside of lid has one
The sieve of the entire inner section of lid is covered, cap top has the liquid outlet of a protrusion, has a frosted in cartridge body and lid
Or label paper paste area.
Further, the suction nozzle of tubular body is an integral structure with tubular body, and material is hard transparent plastic.
The utility model has the beneficial effects that paramagnetic particle method rapidly extracting nucleic acid equipment is simple, easy to operate, price is low.
Detailed description of the invention
Fig. 1 is the syringe-like main body schematic diagram described in the utility model for paramagnetic particle method rapidly extracting nucleic acid；
Fig. 2 is the annular magnet schematic diagram described in the utility model for paramagnetic particle method rapidly extracting nucleic acid.
Fig. 3 is the closed sample filter cartridge schematic diagram described in the utility model for paramagnetic particle method rapidly extracting nucleic acid.
In figure, 1. syringe-like main bodys, 2. annular magnets, 3. sample filtering boxes, 11. tubular bodies, 12. push rods, 13. inhale
Head, 14. suction nozzle caps, 15. annular slots, 16. scarves, 21. half-ring-shaped magnets, 22. semi-circular non-magnetic materials, 23. shafts,
24. buckle, 31. cartridge bodies, 32. lids, 33. sieves, 34. liquid outlets, 35. frosteds or label paper paste area.
Specific embodiment
The utility model is described in detail With reference to embodiment.
The utility model includes syringe-like main body 1, annular magnet 2, sample filtering box 3 as shown in Figures 1 to 3.
Syringe-like main body 1 includes tubular body 11, push rod 12, and the suction nozzle 13 and tubular body of tubular body 11 are one
Body formula structure, material are hard transparent plastic, and suction nozzle 13 is sealed by a matched suction nozzle cap 14, and the bottom end of suction nozzle cap 14 is rubber
Glue material matter plug is for sealing suction nozzle 13.Suction nozzle 13 is equipped with an annular slot 15, and one end of annular slot 15, which is equipped with, hinders push rod
The scarf 16 that 12 piston pushes away to the lower part.
Annular magnet 2 includes half-ring-shaped magnets 21 and semi-circular non-magnetic material 22, and half-ring-shaped magnets 21 and semi-circular are non-
22 one end of magnetic material is connected by shaft 23, the other end can be connected or be split, by 24 connection of buckle when connection.Annular magnet
2 upon connection annular diameters it is identical as 15 outer diameter of annular slot.Annular magnet 2 can just be buckled in syringe-like main body 1
Annular slot 15 in.
Closed sample filter cartridge 3 includes cartridge body 31, lid 32, and the screw thread on lid 32 can be with cartridge body 31
Inner wall is screwed sealing, and there is the sieve 33 of an entire inner section of covering lid in the inside of lid 32, has one at the top of lid 32
The liquid outlet 34 of protrusion, the material for constituting liquid outlet 34 is the rubber material that can be injected the suction nozzle 13 of device sample main body 1 and penetrate.
There are a frosted or label paper paste area 35 in cartridge body 31 and lid 32.
It is operated in this way when extracting sample nucleic acid using the utility model：
1, the lid 32 for unscrewing closed sample filter cartridge 3, a few samples are placed in cartridge body 31, syringe-like is used
Pre-configured nucleic acid releasing agents are transferred in cartridge body 31 by main body 1, tighten lid 32.
2, it by after 3 shake vigorously 1min of sample filtering box, is placed in boiling water and is incubated for 5min.
3, the suction nozzle 13 of syringe-like main body 1 is inserted into liquid outlet 34, and suction nozzle 13 not break sieve 33.?
Sample filtering box 3 is set, the liquid sample after being incubated in box is drawn onto tubular body 11.
4, preprepared magnetic bead liquid is drawn onto tubular body 11 again, air is discharged, covers suction nozzle cap 14, fiercely shakes
It shakes and repeatedly mixes, stand 2min.
5, annular magnet 2 is buckled in the annular slot 15 of syringe, after standing magnetic 2min, lightly by syringe
Interior liquid is all released and is discarded.
6, preprepared rinsing liquid is drawn, suction nozzle cap 14 is covered, jiggles and stand 1min afterwards several times, by syringe
Interior liquid is gently all released and is discarded.
7, push rod 12 is pushed and pulled repeatedly, until syringe-like main body 1 is without droplet.
8, annular magnet 2 is removed, and by the distilled water inhalation syringe of 0.1mL, piston is shifted at scarf 16,
Suction nozzle cap 14 is covered, shake vigorously is multiple, and at this moment the DNA of extraction purification is just dissolved in distilled water, finally by all DNA solution
It is transferred in the 1mL centrifuge tube of sterilizing.
The utility model is the principle that paramagnetic particle method is utilized and extracts sample nucleic acid.Nucleic acid releasing agents are incubated for energy in boiling water
The cell membrane of cell and pathogen is capable of the magnetic bead of specific adsorption nucleic acid in annular to discharge nucleic acid in enough lysed samples
It is adsorbed under the action of magnet in syringe-like main body, and other impurities abandoned, it is further purified by rinsing liquid rinsing
Nucleic acid finally removes magnet, and the nucleic acid of purifying is dissolved in distilled water.In the nucleic acid extraction for encountering excrement and soil equal samples
When, the sieve 33 in closed sample filter cartridge 3 is difficult to the impurity digested for filtering in excrement and soil equal samples, in this way with
Magnetic bead is improved to the adsorption efficiency of nucleic acid.The device for allowing for the paramagnetic particle method rapidly extracting nucleic acid of the utility model in this way can answer
The nucleic acid extraction of nearly all sample is used, the laboratory equipments such as centrifuge have truly been completely disengaged.
The device of the paramagnetic particle method rapidly extracting nucleic acid of the utility model is easy to operate, and whole process only about needs 20 points
Clock, thus the utility model can be widely used in the relatively poor basic hospital and economics of underdevelopment of some experiment conditions
Area or country；It can also be applied to the tentative diagnosis that ordinary populace carries out disease to itself in conjunction with nucleic acid isothermal amplification technique,
Greatly protect the privacy of patient；It is also possible to meet the needs of high-level hospital or large-scale plant that raises well, especially
It is that emergency ward and extensive epidemic disease nucleic acid quickly detect.
The advantages of the utility model, also resides in without any instrument and equipment, it will be able to the core of rapidly extracting multiple types sample
Acid, it is easy to operate, it is cheap, public or basic hospital can be widely used in and carry out nucleic acid quick diagnosis disease, use is close
The design of envelope formula, structure is simple, operation is quick, practical, can not only avoid the Aerosol Pollution in environment, in order to avoid cause core
The false positive of acid detection, and can be avoided pathogen contamination environment and operator in sample, avoid the propagation of disease.
The above is only the better embodiment to the utility model, not makees any form to the utility model
On limitation, all according to the technical essence of the utility model any simple modification made to the above embodiment, equivalent variations
With modification, it is all within the scope of the technical scheme of the utility model.
Claims (2)
1. a kind of device of paramagnetic particle method rapidly extracting nucleic acid, it is characterised in that：Including syringe-like main body (1), annular magnet
(2), sample filtering box (3)；Syringe-like main body (1) includes tubular body (11), push rod (12), and suction nozzle (13) is matched by one
Suction nozzle cap (14) sealing, suction nozzle (13) are equipped with an annular slot (15), and one end of annular slot (15), which is equipped with, hinders push rod (12)
The scarf (16) that pushes away to the lower part of piston, annular magnet (2) includes half-ring-shaped magnets (21) and semi-circular non-magnetic material
(22), half-ring-shaped magnets (21) and semi-circular non-magnetic material (22) one end are connected by shaft (23), the other end can connect or
Person splits, by buckling (24) connection when connection, annular magnet (2) annular diameters and annular slot (15) outer diameter upon connection
Identical, closed sample filtering box (3) includes cartridge body (31), lid (32), and the screw thread on lid (32) can be with box
Main body (31) inner wall is screwed sealing, and there is the sieve (33) of an entire inner section of covering lid in the inside of lid (32), lid
There is the liquid outlet (34) of a protrusion at the top of sub (32), there is a frosted or label paper to paste in cartridge body (31) and lid (32)
Area (35).
2. according to a kind of device of paramagnetic particle method rapidly extracting nucleic acid described in claim 1, it is characterised in that：The tubular body
(11) suction nozzle (13) is an integral structure with tubular body, and material is hard transparent plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721793201.7U CN208121116U (en) | 2017-12-20 | 2017-12-20 | A kind of device of paramagnetic particle method rapidly extracting nucleic acid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721793201.7U CN208121116U (en) | 2017-12-20 | 2017-12-20 | A kind of device of paramagnetic particle method rapidly extracting nucleic acid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208121116U true CN208121116U (en) | 2018-11-20 |
Family
ID=64209332
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721793201.7U Expired - Fee Related CN208121116U (en) | 2017-12-20 | 2017-12-20 | A kind of device of paramagnetic particle method rapidly extracting nucleic acid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208121116U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760194A (en) * | 2021-01-06 | 2021-05-07 | 常州市疾病预防控制中心 | Closed simple RNA nucleic acid extraction device and preparation method and use method thereof |
-
2017
- 2017-12-20 CN CN201721793201.7U patent/CN208121116U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112760194A (en) * | 2021-01-06 | 2021-05-07 | 常州市疾病预防控制中心 | Closed simple RNA nucleic acid extraction device and preparation method and use method thereof |
| CN112760194B (en) * | 2021-01-06 | 2022-11-25 | 常州市疾病预防控制中心 | Closed simple RNA nucleic acid extraction device and preparation method and use method thereof |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181120 Termination date: 20211220 |
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| CF01 | Termination of patent right due to non-payment of annual fee |