CN212955136U - Device for quickly separating exosomes - Google Patents
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- CN212955136U CN212955136U CN202020547718.3U CN202020547718U CN212955136U CN 212955136 U CN212955136 U CN 212955136U CN 202020547718 U CN202020547718 U CN 202020547718U CN 212955136 U CN212955136 U CN 212955136U
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Abstract
A device for quickly separating exosome belongs to the field of biological detection equipment and comprises a separation device body, a screw, a piston, a liquid inlet, a liquid outlet, a liquid inlet pipe, a liquid outlet pipe, a filter membrane component, an ultrafiltration membrane component, a liquid inlet pipe one-way valve and a liquid outlet pipe one-way valve; the separating device main body is a cylindrical pipe body, and the cylindrical pipe body comprises a pipe body upper part and a pipe body lower part; the screw is arranged in the middle of the separating device main body, between the upper part of the tube body and the lower part of the tube body, and the upper part of the tube body and the lower part of the tube body are tightly connected with each other by screwing the screw; the piston is arranged at the upper part of the tube body, the liquid inlet is arranged at one side below the lower part of the tube body, and the liquid outlet is arranged at the other side below the lower part of the tube body; the liquid inlet pipe is arranged below the liquid inlet; the liquid outlet pipe is arranged below the liquid outlet; the filter membrane component is arranged in the liquid inlet, and the ultrafiltration membrane component is arranged in the liquid outlet; the liquid inlet pipe one-way valve is arranged above the ultrafiltration membrane component, and the liquid outlet pipe one-way valve is arranged below the ultrafiltration membrane component. The utility model has the advantages that: the method is fast and efficient; simple structure, scientific and reasonable, easy operation is convenient.
Description
Technical Field
The utility model relates to a device for separating exosomes, in particular to a device for rapidly separating exosomes, which belongs to the field of biological detection equipment.
Background
Exosomes (exosomes) are bilayer vesicular bodies with a diameter of about 30-100nm, and can be actively secreted by various cells in the body, such as immune cells, stem cells, cardiovascular cells, reticulocytes, platelets, nerve cells, tumor cells and the like, and are widely distributed in body fluids such as peripheral blood, urine, saliva, milk, ascites, amniotic fluid and the like. Exosomes carry a large number of specific proteins, such as cytokines, growth factors, functional mRNAs, miRNAs and other bioactive substances, and participate in physiological processes such as cell communication, cell migration, angiogenesis promotion, anti-tumor immunity and the like in vivo. Since exosomes can be used as biological indicators for diagnosing various diseases on one hand and can also be used as therapeutic means on the other hand, exosomes are likely to be used as natural carriers of drugs for clinical treatment in the future.
The separation and purification of exosomes are always the concerns of researchers, and the acquisition of high-purity exosomes is of great importance to the subsequent research. It is known that at present, people mostly adopt methods such as ultracentrifugation, immunomagnetic beads, ultrafiltration, precipitation or kits to realize the extraction and separation of exosomes. However, the methods in the prior art are often very complicated, either special complicated instruments and equipment are needed, or a large amount of time is needed, and a rapid exosome separation device which has a simple structure and can separate exosomes with high efficiency is not provided in the prior art.
SUMMERY OF THE UTILITY MODEL
In order to solve the separation of exosome in the prior art and need complicated instrument and equipment, defects such as inefficiency, the utility model provides an efficient, simple structure's quick separation exosome device can reach high efficiency, carries out exosome isolated purpose.
In order to realize the purpose, the utility model adopts the technical scheme that: a device for quickly separating exosome can quickly separate exosome from a liquid sample, and comprises a separation device main body, a screw, a piston, a liquid inlet, a liquid outlet, a liquid inlet pipe, a liquid outlet pipe, a filter membrane assembly, an ultrafiltration membrane assembly, a liquid inlet pipe one-way valve and a liquid outlet pipe one-way valve; the separation device main body is a cylindrical pipe body, and the cylindrical pipe body comprises a pipe body upper part and a pipe body lower part; the screw is arranged in the middle of the separating device main body, between the upper part of the tube body and the lower part of the tube body, the upper part of the tube body and the lower part of the tube body are tightly connected with each other by screwing the screw, and the upper part of the tube body and the lower part of the tube body are separated by loosening the screw; the filter membrane component comprises a filter membrane, and the filter membrane can be replaced; the ultrafiltration membrane component comprises an ultrafiltration membrane, and the ultrafiltration membrane can be replaced;
the piston is arranged at the upper part of the pipe body, the liquid inlet is arranged at one side below the lower part of the pipe body, and the liquid outlet is arranged at the other side below the lower part of the pipe body; the liquid inlet pipe is arranged below the liquid inlet, and the upper end of the liquid inlet pipe is fixedly and hermetically connected with the liquid inlet; the liquid outlet pipe is arranged below the liquid outlet, and the upper end of the liquid outlet pipe is fixedly and hermetically connected with the liquid outlet; the filter membrane component is arranged in the liquid inlet, and the ultrafiltration membrane component is arranged in the liquid outlet; the liquid inlet pipe one-way valve is arranged above the filter membrane component and can only be opened upwards, the liquid outlet pipe one-way valve is arranged below the ultrafiltration membrane component and can only be opened downwards;
the utility model takes the thrust of the piston as the driving force, when the piston is pulled upwards, the negative pressure is generated in the main body of the separating device, so that the check valve of the liquid inlet pipe is opened upwards, and the liquid sample firstly passes through the filter membrane component from the liquid inlet pipe to filter large granular substances, such as bacteria, cells and other components; at the moment, the one-way valve of the liquid outlet pipe can be closed under the action of negative pressure in the separation device main body; then, when the piston pushes downwards, the liquid outlet pipe one-way valve is opened, and the liquid inlet pipe one-way valve is closed, at the same time, micromolecules such as sugar, inorganic salt and the like, oligonucleotides and proteins with small molecular weight can be discharged from the liquid outlet pipe through the ultrafiltration membrane component, and the exosome particles can not pass through the ultrafiltration membrane component and are remained in the separation device main body; after all the exosomes are separated, the exosomes are kept at the lower part of the tube body; unscrewing the screw port, and removing the upper part of the tube body, so that the separated exosome can be removed from the lower part of the tube body by using a liquid-moving machine (not shown in the figure);
the diameter of the liquid inlet pipe one-way valve is larger than that of the liquid inlet; the diameter of the liquid outlet pipe one-way valve is larger than that of the liquid outlet.
The time required for separating the exosome each time is 10min, the exosome separated each time is 5-10ml, and the number of the separated exosomes corresponds to the size of the separation device main body.
The liquid sample is serum, urine or cell culture fluid.
The filter membrane is a 2.2 μm filter membrane.
The ultrafiltration membrane is a 100KDa ultrafiltration membrane.
Compared with the prior art, the beneficial effects of the utility model are that:
(1) is quick and efficient, the method can quickly (10min) separate exosomes from a large number of samples (5-10ml according to the size of a separation device main body), and the existing method usually needs 30min or more and can only separate samples below 1ml at a time;
and (2) the structure is simple, scientific and reasonable, the operation is simple and convenient, and the popularization and the application are suitable.
Drawings
FIG. 1 is a schematic diagram of: the utility model discloses main view structure picture.
Description of reference numerals: the device comprises a pipe body upper part 1, a pipe body lower part 2, a screw port 3, a piston 4, a liquid inlet 5, a liquid outlet 6, a liquid inlet pipe 7, a liquid outlet pipe 8, a filter membrane component 9, an ultrafiltration membrane component 10, a liquid inlet pipe one-way valve 11 and a liquid outlet pipe one-way valve 12.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific examples, which are not intended to limit the present invention.
As shown in fig. 1, a device for rapidly separating exosome can rapidly separate exosome from a liquid sample, and comprises a separation device body, a screw port 3, a piston 4, a liquid inlet 5, a liquid outlet 6, a liquid inlet pipe 7, a liquid outlet pipe 8, a filter membrane assembly 9, an ultrafiltration membrane assembly 10, a liquid inlet pipe one-way valve 11 and a liquid outlet pipe one-way valve 12; the separation device main body is a cylindrical pipe body, and the cylindrical pipe body comprises a pipe body upper part 1 and a pipe body lower part 2; the screw port 3 is arranged in the middle of the separating device main body and between the upper pipe body 1 and the lower pipe body 2, the upper pipe body 1 and the lower pipe body 2 are tightly connected with each other through screwing the screw port 3, and the screw port 3 is loosened, so that the upper pipe body 1 and the lower pipe body 2 are separated; the filter membrane module 9 comprises a filter membrane (not shown), which can be replaced; the ultrafiltration membrane module 10 comprises an ultrafiltration membrane (not shown), and an ultrafiltration net can be replaced;
the piston 4 is arranged at the upper part 1 of the pipe body, the liquid inlet 5 is arranged at one side below the lower part 2 of the pipe body, and the liquid outlet 6 is arranged at the other side below the lower part 2 of the pipe body; the liquid inlet pipe 7 is arranged below the liquid inlet 5, and the upper end of the liquid inlet pipe 7 is fixedly and hermetically connected with the liquid inlet 5; the liquid outlet pipe 8 is arranged below the liquid outlet 6, and the upper end of the liquid outlet pipe 8 is fixedly and hermetically connected with the liquid outlet 6; the filter membrane component 9 is arranged in the liquid inlet 5, and the ultrafiltration membrane component 10 is arranged in the liquid outlet 6; the liquid inlet pipe one-way valve 11 is arranged above the filter membrane component 9, the liquid inlet pipe one-way valve 11 can only be opened upwards, the liquid outlet pipe one-way valve 12 is arranged below the ultrafiltration membrane component 10, and the liquid outlet pipe one-way valve 12 can only be opened downwards;
the utility model takes the thrust of the piston 4 as the driving force, when the piston 4 is pulled upwards, the negative pressure is generated in the main body of the separation device, so that the check valve 11 of the liquid inlet pipe is opened upwards, and the liquid sample firstly passes through the filter membrane component 9 from the liquid inlet pipe 7 to filter large granular substances, such as bacteria, cells and other components; at the moment, the one-way valve 12 of the liquid outlet pipe is closed under the action of negative pressure in the separation device body; then, when the piston 4 pushes downwards, the liquid outlet pipe one-way valve 12 is opened, and the liquid inlet pipe one-way valve 11 is closed, at the same time, small molecules such as sugar, inorganic salt and the like, oligonucleotides and proteins with small molecular weight are discharged from the liquid outlet pipe 8 through the ultrafiltration membrane component 10, and the exosome particles cannot pass through the ultrafiltration membrane component 10 and are remained in the separation device body; after all the exosomes are separated, the exosomes are kept at the lower part 2 of the tube body; the screw opening 3 is unscrewed, and the upper part 1 of the tube body is removed, so that the separated exosome can be removed from the lower part 2 of the tube body by a liquid transfer device (not shown in the figure);
the diameter of the liquid inlet pipe one-way valve 11 is larger than that of the liquid inlet 5; the diameter of the liquid outlet pipe one-way valve 12 is larger than that of the liquid outlet 6.
The time required for separating the exosome each time is 10min, the exosome separated each time is 5-10ml, and the number of the separated exosomes corresponds to the size of the separation device main body.
The liquid sample is serum, urine or cell culture fluid.
The filter membrane is a 2.2 μm filter membrane.
The ultrafiltration membrane is a 100KDa ultrafiltration membrane.
The above-mentioned embodiments are only one of the preferred embodiments of the present invention, and the general changes and substitutions performed by those skilled in the art within the technical scope of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. A device for rapidly separating exosomes, which can rapidly separate exosomes from a liquid sample, is characterized in that:
comprises a separation device body, a screw, a piston, a liquid inlet, a liquid outlet, a liquid inlet pipe, a liquid outlet pipe, a filter membrane component, an ultrafiltration membrane component, a liquid inlet pipe one-way valve and a liquid outlet pipe one-way valve; the separation device main body is a cylindrical pipe body, and the cylindrical pipe body comprises a pipe body upper part and a pipe body lower part; the screw is arranged in the middle of the separating device main body, between the upper part of the tube body and the lower part of the tube body, and the upper part of the tube body and the lower part of the tube body are tightly connected with each other through screwing the screw; the filter membrane assembly comprises a filter membrane; the ultrafiltration membrane component comprises an ultrafiltration membrane;
the piston is arranged at the upper part of the pipe body, the liquid inlet is arranged at one side below the lower part of the pipe body, and the liquid outlet is arranged at the other side below the lower part of the pipe body; the liquid inlet pipe is arranged below the liquid inlet, and the upper end of the liquid inlet pipe is fixedly and hermetically connected with the liquid inlet; the liquid outlet pipe is arranged below the liquid outlet, and the upper end of the liquid outlet pipe is fixedly and hermetically connected with the liquid outlet; the filter membrane component is arranged in the liquid inlet, and the ultrafiltration membrane component is arranged in the liquid outlet; the liquid inlet pipe one-way valve is arranged above the ultrafiltration membrane component, and the liquid outlet pipe one-way valve is arranged below the ultrafiltration membrane component.
2. A device for rapid separation of exosomes according to claim 1, characterized by: the diameter of the liquid inlet pipe one-way valve is larger than that of the liquid inlet; the diameter of the liquid outlet pipe one-way valve is larger than that of the liquid outlet.
3. A device for rapid separation of exosomes according to claim 1, characterized by: the liquid sample is serum, urine or cell culture fluid.
4. A device for rapid separation of exosomes according to claim 1, characterized by: the filter membrane component is a 2.2 mu m filter membrane component.
5. A device for rapid separation of exosomes according to claim 1, characterized by: the ultrafiltration membrane component is a 100KDa ultrafiltration membrane component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020547718.3U CN212955136U (en) | 2020-04-14 | 2020-04-14 | Device for quickly separating exosomes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202020547718.3U CN212955136U (en) | 2020-04-14 | 2020-04-14 | Device for quickly separating exosomes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN212955136U true CN212955136U (en) | 2021-04-13 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202020547718.3U Expired - Fee Related CN212955136U (en) | 2020-04-14 | 2020-04-14 | Device for quickly separating exosomes |
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| Country | Link |
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| CN (1) | CN212955136U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
-
2020
- 2020-04-14 CN CN202020547718.3U patent/CN212955136U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210413 |